CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP053795	Vibrio cholerae strain W10G chromosome 2, complete sequence	0 crisprs	csa3,cas3	0	0	1	0
NZ_CP053794	Vibrio cholerae strain W10G chromosome 1, complete sequence	1 crisprs	cas3,DEDDh,DinG,csx1,csa3	0	1	4	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

455127 : 467254

Vibrio_phage(77.78%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000815041.1\|455127_455784_+	response regulator transcription factor	W8CYM9	Bacillus_phage	4.2e-29	34.2
WP_000240569.1\|455786_456671_+	DUF2861 family protein	NA	NA	NA	NA
WP_057568909.1\|456731_457898_+	conjugal transfer protein TraF	NA	NA	NA	NA
WP_000459081.1\|457938_458100_+	YoaH family protein	NA	NA	NA	NA
WP_000503164.1\|458232_458670_+\|transposase	IS200/IS605-like element IS1004 family transposase	Q331U4	Clostridium_botulinum_C_phage	1.9e-22	40.8
WP_057569108.1\|459074_459407_-	hypothetical protein	Q9MCC3	Vibrio_phage	1.3e-26	57.1
WP_001896626.1\|460961_461324_+	DUF1293 family protein	Q783U4	Vibrio_phage	1.6e-35	61.0
WP_175234260.1\|461326_461557_+	hypothetical protein	G8IRU7	Vibrio_phage	9.4e-29	77.3
WP_057569106.1\|461590_461800_+	hypothetical protein	G8IRU8	Vibrio_phage	8.6e-13	58.0
WP_057568659.1\|463960_464146_+	hypothetical protein	Q64EU9	Vibrio_phage	1.4e-11	72.7
WP_001893855.1\|464490_464880_+	hypothetical protein	NA	NA	NA	NA
WP_001896595.1\|465482_466862_+	KAP P-loop	R9TRQ8	Vibrio_phage	2.4e-26	26.4
WP_175234261.1\|466882_467254_-	phage protein	G8IRV3	Vibrio_phage	5.5e-63	94.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP053794

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP053794_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP053794_1

2649224-2649467

Orphan

Consensus_repeat	Method
AAAATGTGCCTCCTTAGCTCAGTCGGTAGAGCAACGGACTGATAATCCG	PILER-CR

2 spacers

The CRISPR arrays of NZ_CP053794_1

>merge|NZ_CP053794|1|2649224-2649467|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAATTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTGTCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAATTGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

>NZ_CP053794|1|1|2649224-2649467|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGG	TCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAA
TTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTG	TCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAAT
TGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP053794.1\|WP_000780522.1\|2655847_2657143_+\|peptidylprolyl-isomerase-SurA	unknown	unknown	gnl\|CDD\|236758
NZ_CP053794.1\|WP_000805054.1\|2644426_2645347_-\|glutaminase-B	unknown	unknown	gnl\|CDD\|234880
NZ_CP053794.1\|WP_001888163.1\|2646416_2647478_+\|A/G-specific-adenine-glycosylase	unknown	unknown	gnl\|CDD\|182805
NZ_CP053794.1\|WP_000954087.1\|2660884_2661358_-\|threonine/serine-exporter-family-protein	unknown	unknown	gnl\|CDD\|182143
NZ_CP053794.1\|WP_000546924.1\|2652430_2653285_-\|co-chaperone-DjlA	unknown	unknown	gnl\|CDD\|236512
NZ_CP053794.1\|WP_057567704.1\|2649758_2651384_+\|methyl-accepting-chemotaxis-protein	unknown	unknown	gnl\|CDD\|223910
NZ_CP053794.1\|WP_053034664.1\|2641573_2641864_+\|YggU-family-protein	unknown	unknown	gnl\|CDD\|179931
NZ_CP053794.1\|WP_057567701.1\|2642410_2643013_+\|XTP/dITP-diphosphatase	unknown	unknown	gnl\|CDD\|234648
NZ_CP053794.1\|WP_001087261.1\|2641016_2641574_+\|YggT-family-protein	unknown	unknown	gnl\|CDD\|223833
NZ_CP053794.1\|WP_002046367.1\|2641909_2642341_+\|DUF4426-domain-containing-protein	unknown	unknown	gnl\|CDD\|379616
NZ_CP053794.1\|WP_000005581.1\|2645457_2646177_-\|tRNA-(guanosine(46)-N7)-methyltransferase-TrmB	unknown	unknown	gnl\|CDD\|234649
NZ_CP053794.1\|WP_001277598.1\|2653443_2655795_+\|LPS-assembly-protein-LptD	unknown	unknown	gnl\|CDD\|235158
NZ_CP053794.1\|WP_000095775.1\|2657132_2658125_+\|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA	unknown	unknown	gnl\|CDD\|234696
NZ_CP053794.1\|WP_000124738.1\|2647491_2647764_+\|oxidative-damage-protection-protein	unknown	unknown	gnl\|CDD\|180059
NZ_CP053794.1\|WP_000035022.1\|2659416_2660226_+\|symmetrical-bis(5'-nucleosyl)-tetraphosphatase	unknown	unknown	gnl\|CDD\|234673
NZ_CP053794.1\|WP_000973535.1\|2660374_2660872_-\|type-3-dihydrofolate-reductase	unknown	unknown	gnl\|CDD\|365932
NZ_CP053794.1\|WP_001243262.1\|2658138_2658954_+\|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	unknown	unknown	gnl\|CDD\|234708
NZ_CP053794.1\|WP_057567705.1\|2659025_2659406_+\|Co2+/Mg2+-efflux-protein-ApaG	unknown	unknown	gnl\|CDD\|180098
NZ_CP053794.1\|WP_057567702.1\|2643006_2644182_+\|radical-SAM-family-heme-chaperone-HemW	unknown	unknown	gnl\|CDD\|235550
NZ_CP053794.1\|WP_057567703.1\|2647865_2648993_+\|membrane-bound-lytic-murein-transglycosylase-MltC	unknown	unknown	gnl\|CDD\|183271

Protein	Function_ID	Function_description	E-value
NZ_CP053794.1\|WP_000780522.1\|2655847_2657143_+\|peptidylprolyl-isomerase-SurA	gnl\|CDD\|236758	PRK10770, PRK10770, peptidyl-prolyl cis-trans isomerase SurA; Provisional.	1.14348e-156
NZ_CP053794.1\|WP_000805054.1\|2644426_2645347_-\|glutaminase-B	gnl\|CDD\|234880	PRK00971, PRK00971, glutaminase; Provisional.	0
NZ_CP053794.1\|WP_001888163.1\|2646416_2647478_+\|A/G-specific-adenine-glycosylase	gnl\|CDD\|182805	PRK10880, PRK10880, adenine DNA glycosylase.	0
NZ_CP053794.1\|WP_000954087.1\|2660884_2661358_-\|threonine/serine-exporter-family-protein	gnl\|CDD\|182143	PRK09917, PRK09917, threonine/serine exporter.	7.45224e-67
NZ_CP053794.1\|WP_000546924.1\|2652430_2653285_-\|co-chaperone-DjlA	gnl\|CDD\|236512	PRK09430, djlA, co-chaperone DjlA.	1.60415e-153
NZ_CP053794.1\|WP_057567704.1\|2649758_2651384_+\|methyl-accepting-chemotaxis-protein	gnl\|CDD\|223910	COG0840, Tar, Methyl-accepting chemotaxis protein [Cell motility and secretion / Signal transduction mechanisms].	2.21461e-57
NZ_CP053794.1\|WP_053034664.1\|2641573_2641864_+\|YggU-family-protein	gnl\|CDD\|179931	PRK05090, PRK05090, hypothetical protein; Validated.	1.28694e-61
NZ_CP053794.1\|WP_057567701.1\|2642410_2643013_+\|XTP/dITP-diphosphatase	gnl\|CDD\|234648	PRK00120, PRK00120, dITP/XTP pyrophosphatase; Reviewed.	1.3568e-127
NZ_CP053794.1\|WP_001087261.1\|2641016_2641574_+\|YggT-family-protein	gnl\|CDD\|223833	COG0762, COG0762, Predicted integral membrane protein [Function unknown].	2.77868e-12
NZ_CP053794.1\|WP_002046367.1\|2641909_2642341_+\|DUF4426-domain-containing-protein	gnl\|CDD\|379616	pfam14467, DUF4426, Domain of unknown function (DUF4426). Members of this entry are found mostly in g-proteobacteria, especially in Vibrio. Strangely enough, there seems to be one eukaryotic homolog in Nematostella vectensis (NEMVEDRAFT_v1g226006), where the PA0388-like domain is fused with a domain homogous to the Methionine biosynthesis protein MetW (see below). In several Pseudomonas species, but also in Vibrio vulnificus and Azotobacter vinelandii PA0388 homologs are genomic neighbors of Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15) and Methionine biosynthesis protein MetW. On the other hand, in most Vibrio species it appears as a part of a conserved operon involved in possible response to stress.	3.90922e-67
NZ_CP053794.1\|WP_000005581.1\|2645457_2646177_-\|tRNA-(guanosine(46)-N7)-methyltransferase-TrmB	gnl\|CDD\|234649	PRK00121, trmB, tRNA (guanine-N(7)-)-methyltransferase; Reviewed.	4.57395e-111
NZ_CP053794.1\|WP_001277598.1\|2653443_2655795_+\|LPS-assembly-protein-LptD	gnl\|CDD\|235158	PRK03761, PRK03761, LPS assembly outer membrane complex protein LptD; Provisional.	0
NZ_CP053794.1\|WP_000095775.1\|2657132_2658125_+\|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA	gnl\|CDD\|234696	PRK00232, pdxA, 4-hydroxythreonine-4-phosphate dehydrogenase; Reviewed.	0
NZ_CP053794.1\|WP_000124738.1\|2647491_2647764_+\|oxidative-damage-protection-protein	gnl\|CDD\|180059	PRK05408, PRK05408, oxidative damage protection protein; Provisional.	5.47988e-59
NZ_CP053794.1\|WP_000035022.1\|2659416_2660226_+\|symmetrical-bis(5'-nucleosyl)-tetraphosphatase	gnl\|CDD\|234673	PRK00166, apaH, symmetrical bis(5'-nucleosyl)-tetraphosphatase.	5.07117e-169
NZ_CP053794.1\|WP_000973535.1\|2660374_2660872_-\|type-3-dihydrofolate-reductase	gnl\|CDD\|365932	pfam00186, DHFR_1, Dihydrofolate reductase.	1.40644e-92
NZ_CP053794.1\|WP_001243262.1\|2658138_2658954_+\|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	gnl\|CDD\|234708	PRK00274, ksgA, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA.	3.27746e-146
NZ_CP053794.1\|WP_057567705.1\|2659025_2659406_+\|Co2+/Mg2+-efflux-protein-ApaG	gnl\|CDD\|180098	PRK05461, apaG, CO2+/MG2+ efflux protein ApaG; Reviewed.	3.28286e-73
NZ_CP053794.1\|WP_057567702.1\|2643006_2644182_+\|radical-SAM-family-heme-chaperone-HemW	gnl\|CDD\|235550	PRK05660, PRK05660, radical SAM family heme chaperone HemW.	0
NZ_CP053794.1\|WP_057567703.1\|2647865_2648993_+\|membrane-bound-lytic-murein-transglycosylase-MltC	gnl\|CDD\|183271	PRK11671, mltC, membrane-bound lytic murein transglycosylase MltC.	8.82303e-157

>NZ_CP053794.1|WP_057567703.1|2647865_2648993_+|membrane-bound-lytic-murein-transglycosylase-MltC
MRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINSFSGNIEKRWGRSELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDIELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYDIPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLKEVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
>NZ_CP053794.1|WP_000124738.1|2647491_2647764_+|oxidative-damage-protection-protein
MARTVFCTRLQKEADGLDFQLYPGELGKRIFDNICKEAWAQWQTKQTMLINEKKLNMMDPEHRKLLEQEMVNFLFEGKEVHIEGYTPPAK
>NZ_CP053794.1|WP_001888163.1|2646416_2647478_+|A/G-specific-adenine-glycosylase
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVQALAAAPQDEVLHFWTGLGYYARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQLWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTEKPVKATWFVMLYHDNAVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSKGLWYNLSQPDEIGLAAPVKQLLHSLPFDIDSHI
>NZ_CP053794.1|WP_000005581.1|2645457_2646177_-|tRNA-(guanosine(46)-N7)-methyltransferase-TrmB
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLIIGSGVFHMATDWENYAEHMIEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
>NZ_CP053794.1|WP_000805054.1|2644426_2645347_-|glutaminase-B
MKPTAEILASIIEEVRPLTGQGKVADYIPALAKVPSEKLGIAVFTNQGEVISAGDAQEGFSIQSISKVLSLTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPLIAYDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSKQTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF
>NZ_CP053794.1|WP_057567702.1|2643006_2644182_+|radical-SAM-family-heme-chaperone-HemW
MLTPPPLSLYIHIPWCVQKCPYCDFNSHAQKGEIPEQEYLDALLEDLDRDIERYLLKGDPRLLHSIFIGGGTPSLISAEGIARLLQGVAERIVFKPDIEITMEANPGTIEAQRFAGYRTAGVTRISIGVQSFEPEKLARLGRIHGQQEAVRAAQLAHQIGLNSFNLDLMHGLPDQTPEQALADLDQAIALNPPHLSWYQLTIEPNTLFYSKPPKLPDEDALWDIFELGHQKLSDAGYVQYEISGYSKPGYQCQHNLNYWRFGDYLGIGCGAHGKLSFADGRIVRTTKTKHPRGYLAALNNLAKAYLDSEQLVADQDKPFEFFMNRFRLIEPCPKADFTATTGLTIDVIRPTLDWALSEGYLSEDDQHWQITEKGKLFLNDLLEAFMADEEE
>NZ_CP053794.1|WP_057567701.1|2642410_2643013_+|XTP/dITP-diphosphatase
MKKIVLATGNQGKVREMADLLSDFGFDVVAQSEFNVPEVAETGTTFIENAIIKARHASQITGLPAIADDSGLEVDYLNGAPGIYSARYAGEHASDGDNLNKLLVAMQDVPDDQRSARFHCVLVLMRHADDPTPIVCHGKWEGKILTAPHGSNGFGYDPIFWVPEENCASAELEPVRKKQLSHRGKALQKLFKAIEEQRTC
>NZ_CP053794.1|WP_002046367.1|2641909_2642341_+|DUF4426-domain-containing-protein
MRKWMMSLLLTLLALPASAEQLKTIKDIEVHYSAFNSTFLTPKVASSYQLTRNGYTAILNISVLDRASLGKPATEAKLTGHAKNLIGNLRELSFKQVKEGNAIYYLAEVPISNEEMLTFDIDVDAGLKGAGKLTFSQKFYTEQ
>NZ_CP053794.1|WP_053034664.1|2641573_2641864_+|YggU-family-protein
MAAVWREGDDLLLRLYIQPKASRDSIVGLHGEELKVAITAPPIDGKANAHLSKFLAKQCKVAKGSVVIEKGELGRHKQVRILQPSQIPPEIAALIE
>NZ_CP053794.1|WP_001087261.1|2641016_2641574_+|YggT-family-protein
MNSLSFLINTLFDLYIMVVILRIWLQAARADFYNPFSQFIVKATQPVVGPLRRVIPSIGSIDLATIVFAYVLCVLKFMALVLIASSGSVSFSADFLFLGLLSLIKAAGGLLFWVLLIRAILSWVSQGRSPIEYVFHQLTEPMLAPIRRIIPVMGGFDLSILVLFIVLQFANFLMGDVIGPIWYQL
>NZ_CP053794.1|WP_057567704.1|2649758_2651384_+|methyl-accepting-chemotaxis-protein
MKLKTQAYLLSAIILAALLALTATGLWTLRVASNLDNKARVTELFNSAYSILTEVEKLAQEGKMSEPEAKALATRLMRNNLYKDNEYVYVADENMTFVATPLDPQLHDTSFHDFKDGKGNSVGRLIQDVLRHQSGKLVEYTWTQKQADGSIEEKLSIARKTPHWGWVVGTGIGFNEVNERFWSTAQWQLSLCVVIAVAILSLLLVAIRKILLIIGGEPNEVRSAVQAVAQGRIRREFAIQAPKESIYGAVQQMSSSLADLVAKLEQSMVALRSELAGAGTRAKSIAELTDSQQQSTAMIATAMTEMASSASQVADSARDTAFNTDQADQQSQHTQKLIHNTVSNIQGLATQLQTASTAVADLDLDVKNIAKVLDVIGDIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRNLAGRTQTSTKEIQQMIHNLQEGSRNAIQTIQICGQTSQSSVQESENAASALALIVSALESVSSMSHQIATAAAEQTQVSDDIARRINMIEESGSKLSRVVIESHNSTQTLTKLARELEQWAAHFEVTR
>NZ_CP053794.1|WP_000546924.1|2652430_2653285_-|co-chaperone-DjlA
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVTKEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQVLHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRKLMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK
>NZ_CP053794.1|WP_001277598.1|2653443_2655795_+|LPS-assembly-protein-LptD
MSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGNVVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKAVYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGYSFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFEIDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPEAMKYLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSVAGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDSNYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASGYIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVGFTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMAGVDSAGNSLGYGRPFFLNN
>NZ_CP053794.1|WP_000780522.1|2655847_2657143_+|peptidylprolyl-isomerase-SurA
MKLWKPTLISVLSALTLFNAHAEPKQLDSVAVIVNSGVILQSDVDSALKTIKANAKQNKQPLPQETVLREQVLEKLIIDTLQQQEADRIGVKIDDNRLNEAIKEIAKNNQQTQEQLIASVAQEGLTYPEFREQVRKEMAASDARNALVRRRINILPAEVDTLAELLAQETDATVQYKISHIQLRVDDGQDKSAAETLANKLVNDLRNGADFAQMAYAYSKGPKALQGGDWGWMRKEEMPTIFADQIKMQNKGSIIGPFRSGVGFHILKIDDVKGLETVAVTEVNARHILIKPTIILSDEGAQKQLNEFVQRIKNGEVTFAELAQQYSQDPGSAAQKGELGYQTPDLYVPEFKHQIETLPVGQISEPFKTVHGWHIVEVLDRREVDRTDSALKNKAYRILFNRKFNEEASAWLQELRASAFVEVLKDEKDEQ
>NZ_CP053794.1|WP_000095775.1|2657132_2658125_+|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA
MSSKRIIVTAGEPAGIGPDLVLALSAQDWPHQLVVCADKALLAQRATQLGIQVKLLDYQRDNPVQAQQAGTLLVEHISLAEPVVAGQLNPANGHYVLKTLERAAKGCMNGEFDAIVTGPVHKGVINRAGVAFSGHTEFFAEQSKTPLVVMMLATEGLRTALVTTHLPLAEVPQAITCERLEQIVHILHKDLVEKFAIAEPKIYVCGLNPHAGEDGVLGMEEIETITPTLQRLREQYGMQLVGPLPADTIFSEKYLQQADAVLGMYHDQVLPVLKYKGFGRSVNITLGLPFIRTSVDHGTALDLAGTGQADAGSFWTALAYAIELVDKKAQ
>NZ_CP053794.1|WP_001243262.1|2658138_2658954_+|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
>NZ_CP053794.1|WP_057567705.1|2659025_2659406_+|Co2+/Mg2+-efflux-protein-ApaG
MDVSLPCIKIQVQTRYIEEQSNPEYQRFVFAYLITIKNLSSQTVQLMSRRWLITDAYGKQTVVEGDGVVGEQPRIKANDEYTYSSGTALDTPVGVMQGQYLMIDEQGESFTVEIEPFRLAVPHVLN
>NZ_CP053794.1|WP_000035022.1|2659416_2660226_+|symmetrical-bis(5'-nucleosyl)-tetraphosphatase
MANYIVGDIQGCFDELQQLLKQAEFNSQLDTLWFAGDLVARGPKSLETLRFAYQLGDAARVVLGNHDLHLLSVALGHHSAKRRDQTQAILDAPDAAPLLDWLRQQPLLAEHQEFVLCHAGISPQWDLATARQAAQEVESVLRSPEWSTLIEQMYSDQPDAWHPTLQGIDRLRYIVNAFTRMRFCFPDGRLDMQCKLPPKEVTDGSLLPWFQLPQRIALEKTVIFGHWAALEGYVSETVIGLDTGCVWGGTLTMLRWEDKHYFSQAALPA
>NZ_CP053794.1|WP_000973535.1|2660374_2660872_-|type-3-dihydrofolate-reductase
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSIEAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQYPLPT
>NZ_CP053794.1|WP_000954087.1|2660884_2661358_-|threonine/serine-exporter-family-protein
MLSWDLFFGLLNDMFFAAIPAVGFALVFNVPVPALKYCALGGALGHGSRYLMMHFGVPIEWASFFAATLVGMVGVYWSRRFLAHPKVFTVAALIPMVPGVFAFKAMIALVEINHVGFSPELMEALLENFLKAMFIIAGLAVGLAVPGLLFYRRKPII

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP053794_1	1.1\|2649273\|37\|NZ_CP053794\|PILER-CR	2649273-2649309	37	NC_049942	Escherichia phage JLK-2012, complete sequence	23524-23560	4	0.892
NZ_CP053794_1	1.1\|2649273\|37\|NZ_CP053794\|PILER-CR	2649273-2649309	37	NC_021742	Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence	35428-35464	4	0.892

1. spacer 1.1|2649273|37|NZ_CP053794|PILER-CR matches to NC_049942 (Escherichia phage JLK-2012, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
caggtcgccagttcgattccggtagccggcaccatat	Protospacer
.*********************.***********. *

2. spacer 1.1|2649273|37|NZ_CP053794|PILER-CR matches to NC_021742 (Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
taggtcaccagttcgattccggtagccggcaccaatc	Protospacer
******.***************.*********** *.

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

651941 : 658558

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000210573.1\|651941_653075_+	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	1.4e-64	40.4
WP_057567590.1\|653086_654337_+	glutamate-5-semialdehyde dehydrogenase	A0A1X9I5D4	Streptococcus_phage	8.6e-100	49.5
WP_000543544.1\|654436_654886_+	transcriptional regulator NrdR	NA	NA	NA	NA
WP_057567589.1\|654911_656015_+	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	5.9e-44	33.3
WP_000493874.1\|656019_656673_+	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	1.2e-31	38.2
WP_001122865.1\|656713_657823_+	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	6.7e-64	39.8
WP_000864130.1\|658087_658558_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	6.8e-34	48.7

DBSCAN-SWA_2

1542710 : 1569436

Vibrio_phage(71.43%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000503164.1\|1542710_1543148_+\|transposase	IS200/IS605-like element IS1004 family transposase	Q331U4	Clostridium_botulinum_C_phage	1.9e-22	40.8
WP_057569112.1\|1543217_1543733_-	hypothetical protein	NA	NA	NA	NA
WP_057569114.1\|1543729_1544155_-	TIR domain-containing protein	NA	NA	NA	NA
WP_032473312.1\|1544996_1545374_-	hypothetical protein	NA	NA	NA	NA
WP_000371263.1\|1545551_1545719_-	hypothetical protein	NA	NA	NA	NA
WP_000435396.1\|1545718_1545904_-	hypothetical protein	Q64EU9	Vibrio_phage	6.4e-12	72.7
WP_175234178.1\|1545914_1547285_-	toxin	Q64EV0	Vibrio_phage	2.0e-203	79.0
WP_001160099.1\|1547287_1547632_-	DUF2523 domain-containing protein	Q64EV1	Vibrio_phage	2.3e-55	89.5
WP_057569010.1\|1547631_1549050_-	hypothetical protein	G8IRU9	Vibrio_phage	1.8e-141	69.8
WP_000672168.1\|1549183_1549393_-	hypothetical protein	G8IRU8	Vibrio_phage	2.3e-13	59.4
WP_000063788.1\|1549426_1549657_-	hypothetical protein	G8IRU7	Vibrio_phage	4.7e-28	76.0
WP_001284208.1\|1549666_1550005_-	DUF1293 domain-containing protein	Q858R2	Vibrio_phage	4.6e-56	97.3
WP_057569012.1\|1550004_1551114_-	replication initiation factor domain-containing protein	Q8W6E3	Vibrio_phage	8.4e-208	94.0
WP_000502183.1\|1551103_1551307_-	hypothetical protein	E1CJD3	Vibrio_phage	2.7e-35	100.0
WP_001018566.1\|1551317_1551566_-	hypothetical protein	O56843	Vibrio_virus	6.6e-20	61.0
WP_000271218.1\|1551735_1552104_+	hypothetical protein	Q858Q3	Vibrio_phage	3.8e-56	91.7
WP_057569014.1\|1552425_1566048_-	MARTX multifunctional-autoprocessing repeats-in-toxin holotoxin RtxA	B3Y8K3	Vibrio_virus	8.4e-07	100.0
WP_001881196.1\|1566071_1566533_-	RTX toxin-activating lysine-acyltransferase RtxC	NA	NA	NA	NA
WP_057569033.1\|1566558_1566906_-	hypothetical protein	NA	NA	NA	NA
WP_154591785.1\|1567330_1569436_+	RTX toxin T1SS ABC transporter subunit RtxB	W8CYL7	Bacillus_phage	4.9e-39	27.1

DBSCAN-SWA_3

1809074 : 1858357

Vibrio_phage(52.78%)

integrase,plate,portal,tail,tRNA,capsid,terminase

attL 1841658:1841674|attR 1858107:1858123

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_175234184.1\|1809074_1810331_-	Y-family DNA polymerase	I6RSM4	Salmonella_phage	7.3e-91	43.7
WP_175234239.1\|1810330_1810738_-	translesion error-prone DNA polymerase V autoproteolytic subunit	A0A1B1ITQ0	uncultured_Mediterranean_phage	4.7e-23	46.6
WP_002051960.1\|1810810_1811026_-	hypothetical protein	NA	NA	NA	NA
WP_002051958.1\|1811270_1811543_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_148522416.1\|1811643_1812186_+	hypothetical protein	NA	NA	NA	NA
WP_175234186.1\|1812174_1813185_-\|tail	phage tail protein	R9TNM7	Vibrio_phage	6.3e-61	39.8
WP_113598373.1\|1813175_1813382_-\|tail	tail protein X	R9TR63	Vibrio_phage	7.6e-14	52.2
WP_000179074.1\|1813365_1813761_-\|tail	phage tail protein	R9TMP6	Vibrio_phage	4.7e-28	48.1
WP_175234188.1\|1813760_1815542_-\|tail	phage tail protein	R9TRP8	Vibrio_phage	6.9e-111	42.4
WP_114774472.1\|1815645_1815948_-\|tail	phage tail assembly protein	Q75QK8	Wolbachia_phage	9.2e-08	37.8
WP_175234190.1\|1815999_1816515_-\|tail	phage major tail tube protein	R9TR65	Vibrio_phage	1.3e-17	34.9
WP_175234192.1\|1816514_1817981_-\|tail	phage tail sheath subtilisin-like domain-containing protein	R9TMQ0	Vibrio_phage	2.0e-119	47.8
WP_175234194.1\|1818068_1818593_-\|tail	phage tail protein	A9ZT45	Vibrio_virus	8.3e-89	92.0
WP_175234196.1\|1818592_1819864_-	hypothetical protein	Q8HA60	Vibrio_phage	6.8e-129	70.1
WP_175234197.1\|1819835_1820393_-\|tail	phage tail protein I	R9TR33	Vibrio_phage	1.4e-62	63.2
WP_053033977.1\|1820392_1821271_-\|plate	baseplate J/gp47 family protein	R9TML5	Vibrio_phage	1.4e-67	46.2
WP_175234199.1\|1821267_1821609_-	GPW/gp25 family protein	A0A088FV58	Escherichia_phage	4.6e-32	59.5
WP_172781732.1\|1821610_1822258_-\|plate	phage baseplate assembly protein V	R4JMH2	Burkholderia_phage	2.0e-12	37.7
WP_033935876.1\|1822254_1822779_-	hypothetical protein	R9TNK0	Vibrio_phage	1.8e-22	37.0
WP_053033973.1\|1822771_1823377_-\|tail	phage tail protein	R9TR34	Vibrio_phage	7.9e-59	54.2
WP_088127476.1\|1823339_1823690_-	hypothetical protein	R9TMM0	Vibrio_phage	4.9e-29	53.4
WP_175234201.1\|1823682_1823979_-	hypothetical protein	NA	NA	NA	NA
WP_002051907.1\|1824031_1826011_-\|capsid	phage major capsid protein	A0A1W6JT82	Escherichia_phage	1.4e-157	46.6
WP_175234203.1\|1825967_1827536_-\|portal	phage portal protein	A0A088FVG5	Escherichia_phage	4.3e-117	42.5
WP_000236573.1\|1827535_1828063_-	hypothetical protein	NA	NA	NA	NA
WP_175234241.1\|1828056_1830003_-\|terminase	phage terminase large subunit family protein	A2I2W6	Vibrio_virus	1.5e-215	59.5
WP_113609152.1\|1830013_1830583_-\|terminase	terminase small subunit	B0ZSE9	Halomonas_phage	2.2e-26	38.8
WP_095467472.1\|1831027_1832257_-\|integrase	tyrosine-type recombinase/integrase	A0A067ZJC5	Vibrio_phage	6.3e-55	33.4
WP_175234204.1\|1832716_1832965_-	hypothetical protein	NA	NA	NA	NA
WP_057568767.1\|1833070_1833385_-	hypothetical protein	NA	NA	NA	NA
WP_175234206.1\|1833423_1833912_-	hypothetical protein	A0A2I7R3H9	Vibrio_phage	1.1e-18	37.6
WP_002051883.1\|1833889_1834180_-	hypothetical protein	NA	NA	NA	NA
WP_053033962.1\|1834364_1834946_-	hypothetical protein	NA	NA	NA	NA
WP_053033960.1\|1834947_1835580_-	hypothetical protein	A0A2I7RNS2	Vibrio_phage	1.7e-64	59.3
WP_114774490.1\|1835579_1836296_-	ATP-binding protein	A0A1W6JP39	Morganella_phage	3.0e-41	38.1
WP_175234242.1\|1836307_1837078_-	helix-turn-helix domain-containing protein	A0A1W6JP36	Morganella_phage	2.8e-08	25.2
WP_053033958.1\|1837281_1837827_-	hypothetical protein	NA	NA	NA	NA
WP_032468855.1\|1837866_1838121_-	hypothetical protein	NA	NA	NA	NA
WP_002051862.1\|1838222_1838921_+	transcriptional repressor, CI/C2 family	D4HTY2	Vibrio_phage	1.1e-19	47.6
WP_053033957.1\|1839565_1839943_+	hypothetical protein	NA	NA	NA	NA
WP_053033956.1\|1840006_1840240_+	hypothetical protein	K4I1E3	Acidithiobacillus_phage	1.1e-08	50.8
WP_175234208.1\|1840243_1840978_+	hypothetical protein	A0A067ZJD1	Vibrio_phage	4.1e-102	75.8
WP_175234210.1\|1841015_1842017_+	nucleoid-associated protein YejK	A0A1V0E8C0	Vibrio_phage	1.3e-77	44.4
1841658:1841674	attL	NA	NA	NA	NA
WP_175234212.1\|1842026_1842413_+	hypothetical protein	NA	NA	NA	NA
WP_175234214.1\|1842466_1844086_+	DGQHR domain-containing protein	NA	NA	NA	NA
WP_172896263.1\|1844120_1844381_+	hypothetical protein	R9TNM3	Vibrio_phage	3.5e-16	55.3
WP_002051850.1\|1844515_1844812_+	hypothetical protein	NA	NA	NA	NA
WP_175234216.1\|1844808_1846002_-\|integrase	tyrosine-type recombinase/integrase	K7P7J2	Enterobacteria_phage	1.2e-103	47.6
WP_057568755.1\|1846183_1847608_+	exodeoxyribonuclease I	NA	NA	NA	NA
WP_001881007.1\|1847639_1848014_+	CidA/LrgA family protein	NA	NA	NA	NA
WP_000266242.1\|1848004_1848679_+	LrgB family protein	NA	NA	NA	NA
WP_001245905.1\|1848848_1849736_+	cytidine deaminase	NA	NA	NA	NA
WP_000462871.1\|1849894_1850770_+	AEC family transporter	NA	NA	NA	NA
WP_057568754.1\|1850923_1852099_+	phosphoribosylglycinamide formyltransferase 2	NA	NA	NA	NA
WP_001205535.1\|1852191_1852848_-	thiopurine S-methyltransferase	NA	NA	NA	NA
WP_000054941.1\|1853316_1854096_+	(p)ppGpp synthetase RelV	NA	NA	NA	NA
WP_001229260.1\|1854224_1854521_-	integration host factor subunit alpha	A0A0H3UZA0	Geobacillus_virus	2.4e-13	41.1
WP_057568753.1\|1854959_1857347_-\|tRNA	phenylalanine--tRNA ligase subunit beta	A0A1L3IZU3	BeAn_58058_virus	1.0e-08	25.6
WP_057568752.1\|1857373_1858357_-\|tRNA	phenylalanine--tRNA ligase subunit alpha	A0A2H4UW22	Bodo_saltans_virus	9.0e-36	38.7
1858107:1858123	attR	NA	NA	NA	NA

DBSCAN-SWA_4

2348422 : 2355615

Anguillid_herpesvirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001162850.1\|2348422_2348851_-	nucleoside-diphosphate kinase	D2E8E1	Anguillid_herpesvirus	1.0e-20	38.4
WP_000107234.1\|2349054_2350344_-	aminopeptidase PepB	Q6GYZ8	Mycoplasma_phage	6.5e-34	34.0
WP_000872176.1\|2350563_2350758_-	Fe-S cluster assembly protein IscX	NA	NA	NA	NA
WP_001124187.1\|2350806_2351145_-	ISC system 2Fe-2S type ferredoxin	NA	NA	NA	NA
WP_001959107.1\|2351158_2353009_-	Fe-S protein assembly chaperone HscA	A0A0N9QXN8	Chrysochromulina_ericina_virus	1.5e-105	40.9
WP_057568960.1\|2353032_2353548_-	co-chaperone HscB	NA	NA	NA	NA
WP_000301571.1\|2353596_2353920_-	iron-sulfur cluster assembly protein IscA	A0A2H4N7N5	Lake_Baikal_phage	3.0e-25	50.5
WP_000331703.1\|2353980_2354364_-	Fe-S cluster assembly scaffold IscU	A0A218MKD1	uncultured_virus	5.4e-53	78.2
WP_000775249.1\|2354400_2355615_-	IscS subfamily cysteine desulfurase	A0A0H3TPH2	Faustovirus	8.8e-33	30.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP053795

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP053794

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection