CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP024950	Helicobacter pylori strain B130A chromosome, complete genome	2 crisprs	cas14j,cas3,c2c9_V-U4	0	2	0	0

1. NZ_CP024950

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP024950_1

• CRISPR_ID: NZ_CP024950_1
• CRISPR_location: 577924-578120
• CRISPR_type: Orphan
• Repeat_type: I-B
• Spacer_info: 3 spacers

Consensus repeat of NZ_CP024950_1

Consensus_repeat	Method
TAAAGTGATAAGTCCCTTGATTGAAG	CRISPRCasFinder

spacers of NZ_CP024950_1

>1.1|577950|28|NZ_CP024950|CRISPRCasFinder
GTGTCGTTATTAAAGCTAGTATTGTCAT
>1.2|578004|31|NZ_CP024950|CRISPRCasFinder
TTGCTGTTTTCAAAAGCAGATTGGGTGCTAT
>1.3|578061|34|NZ_CP024950|CRISPRCasFinder
TTACTATTATCAAAGCTTGAACTTGCGCTGTTAC

CRISPR arrays and Neighbor proteins around NZ_CP024950_1

CRISPR arrays

The CRISPR arrays of NZ_CP024950_1

>merge|NZ_CP024950|1|577924-578120|CRISPRCasFinder
TAAAGCTATAAGTCCCTTGATTGAAGGTGTCGTTATTAAAGCTAGTATTGTCATTAAAATTATAAGTGCCTTGATTGAAATTGCTGTTTTCAAAAGCAGATTGGGTGCTATTAAAGTGATAAGTCCCTTGATTGAAGTTACTATTATCAAAGCTTGAACTTGCGCTGTTACTAAAGTGATAAGTGCCTTGATTGAAG

>NZ_CP024950|1|1|577924-578120|CRISPRCasFinder
TAAAGCTATAAGTCCCTTGATTGAAG	GTGTCGTTATTAAAGCTAGTATTGTCAT
TAAAATTATAAGTGCCTTGATTGAAA	TTGCTGTTTTCAAAAGCAGATTGGGTGCTAT
TAAAGTGATAAGTCCCTTGATTGAAG	TTACTATTATCAAAGCTTGAACTTGCGCTGTTAC
TAAAGTGATAAGTGCCTTGATTGAAG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP024950.1|WP_001115888.1|569900_570107_+|4-oxalocrotonate-tautomerase-family-protein	unknown	unknown	gnl|CDD|129125
NZ_CP024950.1|WP_145940141.1|569156_569738_-|recombination-protein-RecR	unknown	unknown	gnl|CDD|273176
NZ_CP024950.1|WP_145940139.1|567095_568076_-|zinc-metalloprotease-HtpX	unknown	unknown	gnl|CDD|235081
NZ_CP024950.1|WP_145940149.1|589822_591412_-|Hop-family-adhesin-AlpB	unknown	unknown	gnl|CDD|280099
NZ_CP024950.1|WP_145940147.1|584176_584608_+|Sua5/YciO/YrdC/YwlC-family-protein	unknown	unknown	gnl|CDD|376519
NZ_CP024950.1|WP_145940138.1|566552_567095_-|GTP-cyclohydrolase-I-FolE	unknown	unknown	gnl|CDD|129173
NZ_CP024950.1|WP_128041456.1|564825_565629_-|5'/3'-nucleotidase-SurE	unknown	unknown	gnl|CDD|234732
NZ_CP024950.1|WP_145940144.1|578979_579978_-|type-I-glyceraldehyde-3-phosphate-dehydrogenase	unknown	unknown	gnl|CDD|273675
NZ_CP024950.1|WP_145940145.1|580121_580814_+|Bax-inhibitor-1/YccA-family-protein	unknown	unknown	gnl|CDD|198414
NZ_CP024950.1|WP_078299248.1|587473_589018_+|outer-membrane-beta-barrel-protein-HofG	unknown	unknown	gnl|CDD|334957
NZ_CP024950.1|WP_145940148.1|584784_587226_+|TonB-dependent-receptor	unknown	unknown	gnl|CDD|224544
NZ_CP024950.1|WP_145940136.1|564169_564829_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_145940140.1|568014_569160_-|tRNA-pseudouridine(13)-synthase-TruD	unknown	unknown	gnl|CDD|272903
NZ_CP024950.1|WP_145940142.1|570166_571270_-|Hop-family-outer-membrane-protein-HopJ/HopK	unknown	unknown	gnl|CDD|280099
NZ_CP024950.1|WP_145940137.1|565625_566537_-|polyprenyl-synthetase-family-protein	unknown	unknown	gnl|CDD|223220
NZ_CP024950.1|WP_145940151.1|593574_595602_-|UvrD-helicase-domain-containing-protein	unknown	unknown	gnl|CDD|223288
NZ_CP024950.1|WP_161593999.1|589089_589329_+|SsrA-binding-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_145940135.1|563565_564168_-|6-pyruvoyl-tetrahydropterin-synthase-family-protein	unknown	unknown	gnl|CDD|223792
NZ_CP024950.1|WP_145940150.1|591433_592990_-|Hop-family-adhesin-AlpA	unknown	unknown	gnl|CDD|280099
NZ_CP024950.1|WP_145940146.1|580914_584172_+|carbamoyl-phosphate-synthase-large-subunit	unknown	unknown	gnl|CDD|235393

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP024950.1|WP_001115888.1|569900_570107_+|4-oxalocrotonate-tautomerase-family-protein	gnl|CDD|129125	TIGR00013, Probable_tautomerase_K2, 4-oxalocrotonate tautomerase family enzyme. 4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function. [Energy metabolism, Other].	4.5194e-26
NZ_CP024950.1|WP_145940141.1|569156_569738_-|recombination-protein-RecR	gnl|CDD|273176	TIGR00615, Recombination_protein_RecR, recombination protein RecR. All proteins in this family for which functions are known are involved in the initiation of recombination and recombinational repair. RecF is also required. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair].	5.24832e-97
NZ_CP024950.1|WP_145940139.1|567095_568076_-|zinc-metalloprotease-HtpX	gnl|CDD|235081	PRK02870, PRK02870, heat shock protein HtpX; Provisional.	0
NZ_CP024950.1|WP_145940149.1|589822_591412_-|Hop-family-adhesin-AlpB	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	1.41313e-53
NZ_CP024950.1|WP_145940147.1|584176_584608_+|Sua5/YciO/YrdC/YwlC-family-protein	gnl|CDD|376519	pfam01300, Sua5_yciO_yrdC, Telomere recombination. This domain has been shown to bind preferentially to dsRNA. The domain is found in SUA5 as well as HypF and YrdC. It has also been shown to be required for telomere recombniation in yeast.	0.000224161
NZ_CP024950.1|WP_145940138.1|566552_567095_-|GTP-cyclohydrolase-I-FolE	gnl|CDD|129173	TIGR00063, GTP_cyclohydrolase_1, GTP cyclohydrolase I. alternate names: Punch (Drosophila),GTP cyclohydrolase I (EC 3.5.4.16) catalyzes the biosynthesis of formic acid and dihydroneopterin triphosphate from GTP. This reaction is the first step in the biosynthesis of tetrahydrofolate in prokaryotes, of tetrahydrobiopterin in vertebrates, and of pteridine-containing pigments in insects. [Biosynthesis of cofactors, prosthetic groups, and carriers, Folic acid].	1.03932e-106
NZ_CP024950.1|WP_128041456.1|564825_565629_-|5'/3'-nucleotidase-SurE	gnl|CDD|234732	PRK00346, surE, 5'(3')-nucleotidase/polyphosphatase; Provisional.	4.77874e-125
NZ_CP024950.1|WP_145940144.1|578979_579978_-|type-I-glyceraldehyde-3-phosphate-dehydrogenase	gnl|CDD|273675	TIGR01534, Glyceraldehyde-3-phosphate_dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, type I. This model represents glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the enzyme responsible for the interconversion of 1,3-diphosphoglycerate and glyceraldehyde-3-phosphate, a central step in glycolysis and gluconeogenesis. Forms exist which utilize NAD (EC 1.2.1.12), NADP (EC 1.2.1.13) or either (1.2.1.59). In some species, NAD- and NADP- utilizing forms exist, generally being responsible for reactions in the anabolic and catabolic directions respectively. Two Pfam models cover the two functional domains of this protein; pfam00044 represents the N-terminal NAD(P)-binding domain and pfam02800 represents the C-terminal catalytic domain. An additional form of gap gene is found in gamma proteobacteria and is responsible for the conversion of erythrose-4-phosphate (E4P) to 4-phospho-erythronate in the biosynthesis of pyridoxine. This pathway of pyridoxine biosynthesis appears to be limited, however, to a relatively small number of bacterial species although it is prevalent among the gamma-proteobacteria. This enzyme is described by TIGR001532. These sequences generally score between trusted and noise to this GAPDH model due to the close evolutionary relationship. There exists the possiblity that some forms of GAPDH may be bifunctional and act on E4P in species which make pyridoxine and via hydroxythreonine and lack a separate E4PDH enzyme (for instance, the GAPDH from Bacillus stearothermophilus has been shown to posess a limited E4PD activity as well as a robust GAPDH activity). There are a great number of sequences in the databases which score between trusted and noise to this model, nearly all of them due to fragmentary sequences. It seems that study of this gene has been carried out in many species utilizing PCR probes which exclude the extreme ends of the consenses used to define this model. The noise level is set relative not to E4PD, but the next closest outliers, the class II GAPDH's (found in archaea, TIGR01546) and aspartate semialdehyde dehydrogenase (ASADH, TIGR01296) both of which have highest-scoring hits around -225 to the prior model. [Energy metabolism, Glycolysis/gluconeogenesis].	3.23316e-160
NZ_CP024950.1|WP_145940145.1|580121_580814_+|Bax-inhibitor-1/YccA-family-protein	gnl|CDD|198414	cd10432, BI-1-like_bacterial, Bacterial BAX inhibitor (BI)-1/YccA-like proteins. This family is comprised of bacterial relatives of the mammalian members of the BAX inhibitor (BI)-1 like family of small transmembrane proteins, which have been shown to have an antiapoptotic effect either by stimulating the antiapoptotic function of Bcl-2, a well-characterized oncogene, or by inhibiting the proapoptotic effect of Bax, another member of the Bcl-2 family. In plants, BI-1 like proteins play a role in pathogen resistance. A characterized prokaryotic member, Escherichia coli YccA, has been shown to interact with ATP-dependent protease FtsH, which degrades abnormal membrane proteins as part of a quality control mechanism to keep the integrity of biological membranes.	1.83743e-52
NZ_CP024950.1|WP_078299248.1|587473_589018_+|outer-membrane-beta-barrel-protein-HofG	gnl|CDD|334957	pfam02521, HP_OMP_2, Putative outer membrane protein. This family consists of putative outer membrane proteins from Helicobacter pylori (campylobacter pylori).	1.40925e-178
NZ_CP024950.1|WP_145940148.1|584784_587226_+|TonB-dependent-receptor	gnl|CDD|224544	COG1629, CirA, Outer membrane receptor proteins, mostly Fe transport [Inorganic ion transport and metabolism].	4.10665e-35
NZ_CP024950.1|WP_145940140.1|568014_569160_-|tRNA-pseudouridine(13)-synthase-TruD	gnl|CDD|272903	TIGR00094, tRNA_pseudouridine_synthase_D, tRNA pseudouridine synthase, TruD family. an EGAD loading error caused one member to be called surE, but that's an adjacent gene. MJ11364 is a strong partial match from 50 to 230 aa. [Protein synthesis, tRNA and rRNA base modification].	5.67241e-141
NZ_CP024950.1|WP_145940142.1|570166_571270_-|Hop-family-outer-membrane-protein-HopJ/HopK	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	1.30483e-47
NZ_CP024950.1|WP_145940137.1|565625_566537_-|polyprenyl-synthetase-family-protein	gnl|CDD|223220	COG0142, IspA, Geranylgeranyl pyrophosphate synthase [Coenzyme metabolism].	3.50874e-77
NZ_CP024950.1|WP_145940151.1|593574_595602_-|UvrD-helicase-domain-containing-protein	gnl|CDD|223288	COG0210, UvrD, Superfamily I DNA and RNA helicases [DNA replication, recombination, and repair].	1.36733e-136
NZ_CP024950.1|WP_145940135.1|563565_564168_-|6-pyruvoyl-tetrahydropterin-synthase-family-protein	gnl|CDD|223792	COG0720, COG0720, 6-pyruvoyl-tetrahydropterin synthase [Coenzyme metabolism].	5.76282e-22
NZ_CP024950.1|WP_145940150.1|591433_592990_-|Hop-family-adhesin-AlpA	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	3.40148e-54
NZ_CP024950.1|WP_145940146.1|580914_584172_+|carbamoyl-phosphate-synthase-large-subunit	gnl|CDD|235393	PRK05294, carB, carbamoyl-phosphate synthase large subunit.	0

Protein sequences

>NZ_CP024950.1|WP_145940142.1|570166_571270_-|Hop-family-outer-membrane-protein-HopJ/HopK
MQKTLLHSSFFLPLLLSYCIAEENGAYASVGFEYSISHAVEHNNPFLNQERIQIISNAQNKIYKLNQVKNEITSMQNTFNYINNALKNNSKLTPTEIQAEKYYLQSTFQNIEKIVVLSGGVASNPQLAQALEKIQEPITNPLELAENLRNLEVQFAQSQNRMLSSLSSQIAQISNSLNALDPNSYSKNISSMYGVSLSVGYKHFFTKKKNQGLRYYLFYDYGYTNFGFVGNGFDGLGKMNNHLYGLGIDYLYNFIDNAQKHSSVGFYVGFALAGSSWVGSGLGMWVSQRDFINNYLINYQAKMHTSFFQIPLNFGVRVNVNRHNGFEMGLKIPLAVNSFYETHGKGLNTSLFFKRLVAFNVSYVYSF
>NZ_CP024950.1|WP_001115888.1|569900_570107_+|4-oxalocrotonate-tautomerase-family-protein
MPFINIKLVPENGGPTNEQKQQLIEGVSDLMVKVLNKNKASVVVIIDEVDSNNYGLGGESVHHLRQKN
>NZ_CP024950.1|WP_145940141.1|569156_569738_-|recombination-protein-RecR
MNTYKNSLNHFLNLVDCLEKIPNVGKKSAFKMAYHLGLENPYLALKITHALENALENLKTCASCNALSESEVCEICSDESRQNSQLCMVLHPRDVFILEDLKDFLGRYYVLNSIGEVDFNALEKRLIEENIKEIIFAFPPTLANDSLMLYIEDKLQHFQLTFTKIAQGVPTGVNFENIDSVSLSRAFNSRIKA
>NZ_CP024950.1|WP_145940140.1|568014_569160_-|tRNA-pseudouridine(13)-synthase-TruD
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEMLQIFSQILGVKIAELGYAGLKDKNALTTQFISLPKKYAPLLEKNTSNFQERNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAQKTKQVLEQIVQFGMPNYFGSQRFGKFNDNHQEGLKILQNKTKFAHQKLNAFLISSYQSYLFNSLLSKRLEISKIISDFSIKENLEFFKQNNLSVDSNTLKALKNQAHPFKILEGDVMCHYPYGKFFDALELEKEGERFLKKEAAPTGLLDGKKALYAKNLSLEIEKEFQHNLLNSHAKTLGSRRFFWVFAENVTSQYIKEKVQFELGFYLPKGSYASALLKEIKHEKGENNDEF
>NZ_CP024950.1|WP_145940139.1|567095_568076_-|zinc-metalloprotease-HtpX
MRARCSKKSSMRKEKIMTNFEKIIAQNRIKTNAVLTTYCAIFAFIGLLVDAIRINANDLGIALFKLITFQIFPAITIIMFLAAFVVIVVCIQNFSSIMLSGDEYKLIDKSKVLSSKENQIHRLLLELLEEAKLHFEPKLYIINAPYMNAFASGWNESNSLIALTSALIERLDKDELKAVIAHELSHIRHNDIRLTMCVGILSNIMLLVANFSVYFFMGNRKNSGANLARMILWALQIVLPFLTLILQMYLSRTREYMADSGAAFLMHDNKPMIRALQKISNDYANNDYKGVNQNSTRSAAYLFNAEMFSTHPSIKNRIQSLRKRVI
>NZ_CP024950.1|WP_145940138.1|566552_567095_-|GTP-cyclohydrolase-I-FolE
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMIVARNIEFYSTCEHHLLPFLGNISVGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIAETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVLRGLFKKDPKTRAEFMQLLKS
>NZ_CP024950.1|WP_145940137.1|565625_566537_-|polyprenyl-synthetase-family-protein
MSNPNLSFYYNECERFESFLKNHHLHLESFHPYLEKAFFEMVLNGGKRFRPKLFLAVLCALVGQKDYSNQQTEYFKIALSIECLHTYSLIHDDLPCMDNAALRRNHPTLHAKYDETTAVLIGDALNTYSFELLSNSLLESHIIVELIKILSVNGGIKGMILGQALDCYFENTPLNLEQLTFLHEHKTAKLISASLMMGLVASGIKDEELFKWLQAFGLKMGLCFQVLDDIVDVTQDEKESGKTTHLDSAKNSFVNLLGLEKANDYAQTLKTEVLNDLNLLKPAYPLLQENLNALLNTLFKGKT
>NZ_CP024950.1|WP_128041456.1|564825_565629_-|5'/3'-nucleotidase-SurE
MKKILLTNDDGYHAKGIKALEQALENMAEIYVVAPKHEKSACSQCITITAPLRAEKIKGKEGRHYRIDDGTPSDCVYLAINELFKHVCFDLVISGINLGSNMGEDTIYSGTVAGAIEGTIQGVPSIAISQILSNKNKNTPLSFDLAQKIIQDLVQNIFTNGYPLKGRKLLNVNVPNCSLQEYKGERITPKGYRLYKKEVHKRTDPKNESYFWLGLHPLEWQKRENEDRLSDFDAIASNHASITPLNLDLTSYDDLKSLESWHEGMLK
>NZ_CP024950.1|WP_145940136.1|564169_564829_-|hypothetical-protein
MSKKHRLAFLGLIVGVFFFFNACQHRLHMGYYSEVTGDYLFNYNSTIVVAYDRSDAMTSYYIDVIVYELQKLGFYNVFTQAEFPLDKAKNVIYARIVRNISAVPFYQYNYQLIDQVNKPCYFLGGQFYCSQTPTDYYAINGFSEQILMSANSHFILDWYDVVLQKRVLYVDGSVSGRTCGYQMLYRDLIKSTIKRIDFNRPERYYYNLRLPLYQPCYRE
>NZ_CP024950.1|WP_145940135.1|563565_564168_-|6-pyruvoyl-tetrahydropterin-synthase-family-protein
MVIRRLYRFCASHVVRNCSSLKCAQNIHGHNYEVEVFIETNRLDSANMALDFGLMQQEMQVFIESFDHAHHFWDKESDEFQRFIENHCARYVKCSFNLSAESYALMFLYYLTRILQKSVFSNDEGELKVSSVRVHETKNGYAESFLKDLENPHFKSLVHNHCVSFSQGIQNLWHDKDFFNKIISDEKQCFFHTKPLHQIP
>NZ_CP024950.1|WP_145940144.1|578979_579978_-|type-I-glyceraldehyde-3-phosphate-dehydrogenase
MKIFINGFGRIGRCVLRAILERSDTNSHLEVIGINDPANWEILAYLLEHDSVHGLLFKEARYSNYKLIIGSLEIPVFNSIKDLKGVDVILECSGKFLEPKTLENYLLLGAKKVLLSAPFMGEYDEKQYPTLVYGVNHFLYQNQAIVSNASCTTNAIAPICAILDKAFGIKEGMLTTIHSYTSDQKLIDLAHPLDKRRSRAAASNIIPTTTKAALALHKVLPNLKNKMHGHSVRVPSLDVSMIDLSLFLEKKALKDPINDLLIEASKGVLKGVLEIDLKERVSSDFISNPHSVIIAPDLTFTLENMVKIMGWYDNEWGYSNRLVDMAQFVYHY
>NZ_CP024950.1|WP_145940145.1|580121_580814_+|Bax-inhibitor-1/YccA-family-protein
MALYDRADSRNAYAENSLLQESELVSFVKTTYKFFAGSLLLATIGALLGLMNFQAVVQYKWVFFIAEIVALFGLMFSKSKPGLNLFMLFAFTSLSGVTLVPLLGMVIAKAGLGAVWQALGMTTIVFGLMSVYALKTKNDLANMGKMLFIALIVVLVCSLINLFLGSPMFQVVIAGASAILFSLYIAYDTQNIVKGMYDSPIDAAVDLYLDFLNVFISILQIIGIFSDRDK
>NZ_CP024950.1|WP_145940146.1|580914_584172_+|carbamoyl-phosphate-synthase-large-subunit
MPKRTDISNILLIGSGPIVIGQACEFDYSGTQSCKTLKSLGYRVILINSNPATVMTDPEFSHQTYIQPITPENIATIIKKEKIDAILPTMGGQTALNAVMQMHQKGMLEGVELLGAKIEAIKKGEDRQAFKEAMLKIGMDLPKGRYAYNELEALEAISEIGFPAIIRASFTLAGGGSGVAYNIEEFQELAKNALDASPINEILIEESLLGWKEYEMEVIRDSKDNCIIVCCIENIDPMGVHTGDSITIAPSLTLTDKEYQRMRDASFAILREIGVDTGGSNVQFAIHPETLRMVVIEMNPRVSRSSALASKATGFPIAKVATMLAVGFSLDEIKNDITNTPASFEPSLDYIVVKIPRFAFEKFAGVSSTLGTSMKSIGEVMAIGGNFLEALQKALCSLENNWLGFESLSKDLEMIKKEIRRPNPKRLLYIADAFRLGVCVDEVFELCQIDRWFLSQIQKLVKAEGSINSSVLTDAKKLRGLKNLGFSDARIAAKIKENDNLEVSPFEVELARMHLQIAPHFEEVDTCAAEFLSLTPYLYSTYAPNPLPPIENKQEKKEKKILIIGSGPNRIGQGIEFDYCCVHASFALKDLNIKSVMLNCNPETVSTDYDTSDTLYFEPIHFECVKSIIQRERVDGIIVHFGGQTPLKLAKDLAKMQAPIIGTPFKVIDIAEDREKFSLFLKELDIKQPENGMAKSVDEAYSIANVIGFPIIVRPSYVLGGQHMQILENIEELRHYLESVTHALEISPKNPLLIDKFLEKAVELDVDAICDKKEVYIAGILQHIEEAGIHSGDSACFIPSNLNPEILDEIERVSAKIALHLGVVGLLNIQFAVYENSLYLIEVNPRASRTVPFLSKALGVPLAKVATRVMVLEDLKEALKFYDKKNIVEYSNGVYKPKMPHFVALKEAVFPFNKLYGSDLILGPEMKSTGEVMGIARSLGLAFFKAQTACFNPIKNKGLIFVSIKDKDKEEACVLMKRLVQLGFELCATEGTHKALEKAGVKSLKVLKISEGRPNIMDLMMNGEISMAINTSDHKSQDDAKLIRASVLKNHVSYFTTLSAIEVLLLALEESSQEDELLALQDYLK
>NZ_CP024950.1|WP_145940147.1|584176_584608_+|Sua5/YciO/YrdC/YwlC-family-protein
MALVYLAQSDTTIGLLSKDSEKLNALKNRPKNQSVLIESADFTTLKSLVRAPNAFKNLIRRSVKTTFIYPNSKAVRVVRGRHGDFLKRFKTLYSTSANLTQCAYDKEIASKLADVIVSDERGLFESSSSKIFKLYKDKKVRVR
>NZ_CP024950.1|WP_145940148.1|584784_587226_+|TonB-dependent-receptor
MNDKRFRKYWSFSIFLSLLGMFELEAKEEEKEERKTERKKDKKKNAQHTLGKVTTQAAKIFNYNNQTTISSKELERRQANQISDMFRRNPNINVGGGAVIAQKIYVRGIEDRLARVTVDGAAQMGASYGHQGNTIIDPGMLKSVVVTKGAAQASAGPMALIGAIKMETKSASDFIPKGKDYAISGAATFLTNFGDRETVMGAYRNHHFDALLYYTHQNIFYYRDGDNAMKNLFDPKAENKVTGSPSEQNNVMAKINGYLSERDTLTLSYNMTRDNANRPLRANFTGTFLPYSCGDFNAFPNEKNPSDCLFENNASLFKTYSVNLVHNVSLNYEREGGSRFGDPKLKINGYTSIRNVQIDPLFRPSDIAAIIPFTPNPKLGEENECVAQGGIYDATKQTCSITFKSLGGGSVVANKNLFIINSGFNANVIHTIDHKNDNLLEYGLNYQNLTTFDKAIPNSELVRPGDAPDACLRVTSPNDPNMNGRCQRNGATANVVGVYAQANYTLHPMVTLGAGTRYDVYTLVDKDWQLHITQGFSPSAALNVSPLENLNFRLSYAYVTRGPMPGGLVWMRQDNLRYNRNLKPEIGQNVEFNTEYSSEYFDFRAAGFVQLISNYINQFSSTLFVTNLPAQDIIYVPGYEVSGTAKYKGFSLGLSVARSWPSLRGRLIADVYELAATTGNVFILTASYIIPRTGLSITWLSRFVTDLSYCSYSPYRNGPTDIDRRPSDCPKTPGIFHVHKPGYGVSSFFITYKPTYKKLKGLSLNAVFNNVFNQQYIDQASPVMSPDEPNQDKYARGMAEPGFNARFEISYKF
>NZ_CP024950.1|WP_078299248.1|587473_589018_+|outer-membrane-beta-barrel-protein-HofG
MRQEKYFLTSSLSLLSFLLCPVEAFDYRFSGRVENFSKIGFNNSQINTKKGIYPTESFIDIVTLAQVKVNLLPKGTENHRLSVSLGGAIAAIPYDKTKYYINQANGKVFGSIVENFIGGYHGYFFNKYLGPAYAGTSQSASYHARPYVVDTAFLRYDYKDVFGFKAGRYEANIDFMSGSNQGWEVYYQPYKTETQRLRFWWWSSFGRGLAFNSWIYEFFATVPYLKKGGNPSNSNDFINYGWHGITTTYSYKGLDAQFFYYFAPKTYNAPGFKLVYDTNRNFENVGFRSQSMIMTTFPLYYRGWYNPETNTYSLEDSTPHGSLLGRNGVTLNIRQVFWWDNFNWSIGFYNTFGNSDAFLGSHTMPRGNNTSYIGSEISITTRHAGMIGYDFWDNTAYDGLADAITNANTFTFYTSVGGIHKRFAWHVFGRVSHANKNALGQVGRANEYSVQFNASYAFTESVLLNFRITYYGARINKGYQAGYFGAPKFNNPDGDFSANYQDRSYMMTNLTLKF
>NZ_CP024950.1|WP_161593999.1|589089_589329_+|SsrA-binding-protein
MKCSTALAIGLPDVGGEFGFCSVCLIIGILQSLSSNAFLTALTHHIYKGVTLRASYARCKCMGVLYCKLAAFTPNTKDV
>NZ_CP024950.1|WP_145940149.1|589822_591412_-|Hop-family-adhesin-AlpB
MKQNLKPFKMIKENLMTQSQKVRFLAPLSLALSLSFNPVGAEEDGGFMTFGYELGQVVQQVKNPGKIKAEELAGLLNSNTTNNTNINIAGTGGNVAGTLGNLFMNQLGNLIDLYPTLNTSSIHQCGATNSGGSGATTAAATTSNSSCFQGNLSLYKEMVGSIKTLSQNISKNIFQGNNNTTSANLSNQLSELNTASVYLTYMNSFLNANNQAGGIFQNNTDKIYGSGVTAQQIAYVLKQASITMGPSGDSGAAGAFLDAALAQHVFNSANAGNDLSAKEFTSLVQNIVNNSQNALTLANNANISNSTGYQVSYGGNIDQARSTQLLNNTTNTLAKVTALNNELKANPWLGNFAAGNSSQVNAFNGFITKIGYKQFFGENKNVGLRYYGFFSYNGAGVGNGPTYNQVNLLTYGVGTDVLYNVFSRSFGSRSLNAGFFGGIQLAGDTYISTLRNSPQLASRPTATKFQFLFDVGLRMNFGILKKDLKSHNQHSIEIGVQIPTIYNTYYKAGGAEVKYFRPYSVYWVYGYAF
>NZ_CP024950.1|WP_145940150.1|591433_592990_-|Hop-family-adhesin-AlpA
MIKKNRTLFLSLALCASISYAEDDGGFFTVGYQLGQVMQDVQNPGGAKSDELARELNADVTNNILNNNTGGNVAGALSNAFSQYLYSLLGAYPTKLNSNDVSANALLSGAVGSGTCAAAGTAGSTTLNTQSACTAAGYYWLPSLTDRILSTIGSQTNYGTNTNFPNMQQQLTYLNAGNVFFNAMNKALEKNGTANGNSGTSGATGSNGQTYSQQAIQYLQGQQNILNNAANLLKQDELLLEAFNSAVAANIGNKEFNSAAFTGLVQGIIDQSQLVYNELTKNTISGSAVNNAWISSNQANAVQGRASQLPNALYNAQVTLNKINALNNQVRSMPYLPQFRAGNSRATNILNGFYTKIGYKQFFGKKRNIGLRYYGFFSYNGASVGFRSTQNNVGLYTYGVGTDVLYNIFSRSYQNRSVDMGFFSGIQLAGETFQSTLRDDPNVKLHGKINNTHFQFLFDFGMRMNFGKLDGKSNRHNQHTVEFGVVVPTIYNTYYKSAGTTVKYFRPYSVYWSYGYSF
>NZ_CP024950.1|WP_145940151.1|593574_595602_-|UvrD-helicase-domain-containing-protein
MLETLQLNPEQLKAAKALQGHNLVIASAGTGKTSTIVGRILHLLDNGIKPEEILLLTFTNKASNEMIARVAKYFKSSSKIEAGTFHAVAYRYLKEHYPNLSLKQPKELRKLLESIVDTKNAIDDDKKPYTSQHLYALYSLYTNALKQEDFSAWLSSKNPEHTPYAALYENILEEFENTKKKHNYIDYNDLLLLFKKAMLERPSPYKEVLCDEFQDTNPLQESILDAINPPSLFCVGDYDQSIYAFNGADISIISNFTQKYKNAQVFTLTKNYRSSKEILDLANQVIQRNERIYPKNLEVVKSGKFNKPTLLNYNDNIAQCQDIAKRIVMRKNFKEVAVIFRNNASADQLEAALRSHNVPSKRKGSASFFESKEVALALDICALIFNPKDIMAAIHVLSYISDIGSNTAKDIHEALMLLGNGDLKLALTHPNKEAKIYTKKKEITSMGLFEEIFALENSSRFNSVINKAFHSHPVLMHPKISLNGAKTLSDFFTLYTKAPTHSPSALIKHILESAFFQTFKTRLLKERSKNKDGSYNEFKKLQAQKRFNEKMDLLSSLAKNYQNLGRFLNGTLIGSSEATQGEGVNLLSVHASKGLEFKDVYIIDLMEGRFPNHKLMNTGGGIEEERRLFYVAITRAKENLWLSYAKNELRENAKPKEHKPSVFLYEAGLLKPDSK

Crispr_ID: NZ_CP024950_2

• CRISPR_ID: NZ_CP024950_2
• CRISPR_location: 1493959-1494044
• CRISPR_type: TypeV-U4
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: c2c9_V-U4

Consensus repeat of NZ_CP024950_2

Consensus_repeat	Method
AGTCAAGCTTTCGTTATAAGAAAGC	CRISPRCasFinder

spacers of NZ_CP024950_2

>2.1|1493984|36|NZ_CP024950|CRISPRCasFinder
TTTCTTACAATTTTTAACGCAACGATTTTTGGCTAA

CRISPR arrays and Neighbor proteins around NZ_CP024950_2

CRISPR arrays

The CRISPR arrays of NZ_CP024950_2

>merge|NZ_CP024950|2|1493959-1494044|CRISPRCasFinder
AGTCAAGCTTTCGTTATAAGAAAGCTTTCTTACAATTTTTAACGCAACGATTTTTGGCTAAAGTCAAGCTTTCGTTATAAGAAGGC

>NZ_CP024950|2|2|1493959-1494044|CRISPRCasFinder
AGTCAAGCTTTCGTTATAAGAAAGC	TTTCTTACAATTTTTAACGCAACGATTTTTGGCTAA
AGTCAAGCTTTCGTTATAAGAAGGC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP024950.1|WP_161594004.1|1501841_1502033_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_000238762.1|1488753_1489353_+|urease-accessory-protein-UreG	unknown	unknown	gnl|CDD|129208
NZ_CP024950.1|WP_145940661.1|1492626_1492974_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_145940663.1|1494925_1495186_-|type-VII-secretion-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_039093380.1|1483409_1483883_+|lipoprotein-signal-peptidase	unknown	unknown	gnl|CDD|234739
NZ_CP024950.1|WP_145940666.1|1503969_1505214_+|DHH-family-phosphoesterase	unknown	unknown	gnl|CDD|223681
NZ_CP024950.1|WP_145940659.1|1487960_1488725_+|urease-accessory-protein-UreF	unknown	unknown	gnl|CDD|223900
NZ_CP024950.1|WP_000779223.1|1484226_1484943_+|urease-subunit-alpha	unknown	unknown	gnl|CDD|184439
NZ_CP024950.1|WP_145940101.1|1507239_1508130_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_145940660.1|1489352_1490150_+|urease-accessory-protein-UreD	unknown	unknown	gnl|CDD|223899
NZ_CP024950.1|WP_001158316.1|1511299_1511728_-|PaaI-family-thioesterase	unknown	unknown	gnl|CDD|224961
NZ_CP024950.1|WP_000724295.1|1484946_1486656_+|urease-subunit-beta	unknown	unknown	gnl|CDD|184438
NZ_CP024950.1|WP_053577752.1|1508263_1510111_-|arginine-decarboxylase	unknown	unknown	gnl|CDD|224088
NZ_CP024950.1|WP_145940665.1|1502180_1503890_+|DUF262-domain-containing-protein	unknown	unknown	gnl|CDD|224396
NZ_CP024950.1|WP_000901271.1|1486847_1487435_+|acid-activated-urea-channel	unknown	unknown	gnl|CDD|259831
NZ_CP024950.1|WP_145940658.1|1487436_1487949_+|urease-accessory-protein-UreE	unknown	unknown	gnl|CDD|237322
NZ_CP024950.1|WP_145940667.1|1510125_1511295_-|glycosyltransferase	unknown	unknown	gnl|CDD|340844
NZ_CP024950.1|WP_079338282.1|1497039_1497354_-|transposase	c2c9_V-U4	-	gnl|CDD|284650
NZ_CP024950.1|WP_145940664.1|1495201_1495771_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP024950.1|WP_145940662.1|1492975_1493395_-|SMI1/KNR4-family-protein	unknown	unknown	gnl|CDD|378155

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_CP024950.1|WP_079338282.1|1497039_1497354_-|transposase	c2c9_V-U4	-	1.2e-21	71.7	0.1

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP024950.1|WP_000238762.1|1488753_1489353_+|urease-accessory-protein-UreG	gnl|CDD|129208	TIGR00101, Urease_accessory_protein_UreG, urease accessory protein UreG. This model represents UreG, a GTP hydrolase that acts in the assembly of the nickel metallocenter of urease. It is found only in urease-positive species, although some urease-positive species (e.g. Bacillus subtilis) lack this protein. A similar protein, hypB, is an accessory protein for expression of hydrogenase, which also uses nickel. [Central intermediary metabolism, Nitrogen metabolism].	1.6612e-140
NZ_CP024950.1|WP_079338282.1|1497039_1497354_-|transposase	gnl|CDD|284650	pfam07282, OrfB_Zn_ribbon, Putative transposase DNA-binding domain. This putative domain is found at the C-terminus of a large number of transposase proteins. This domain contains four conserved cysteines suggestive of a zinc binding domain. Given the need for transposases to bind DNA as well as the large number of DNA-binding zinc fingers we hypothesize this domain is DNA-binding.	1.80826e-15
NZ_CP024950.1|WP_039093380.1|1483409_1483883_+|lipoprotein-signal-peptidase	gnl|CDD|234739	PRK00376, lspA, lipoprotein signal peptidase.	4.02036e-42
NZ_CP024950.1|WP_145940659.1|1487960_1488725_+|urease-accessory-protein-UreF	gnl|CDD|223900	COG0830, UreF, Urease accessory protein UreF [Posttranslational modification, protein turnover, chaperones].	7.93692e-91
NZ_CP024950.1|WP_000779223.1|1484226_1484943_+|urease-subunit-alpha	gnl|CDD|184439	PRK13986, PRK13986, urease subunit beta.	9.94747e-143
NZ_CP024950.1|WP_145940660.1|1489352_1490150_+|urease-accessory-protein-UreD	gnl|CDD|223899	COG0829, UreH, Urease accessory protein UreH [Posttranslational modification, protein turnover, chaperones].	1.66033e-107
NZ_CP024950.1|WP_001158316.1|1511299_1511728_-|PaaI-family-thioesterase	gnl|CDD|224961	COG2050, PaaI, HGG motif-containing thioesterase, possibly involved in aromatic compounds catabolism [Secondary metabolites biosynthesis, transport, and catabolism].	1.74319e-12
NZ_CP024950.1|WP_000724295.1|1484946_1486656_+|urease-subunit-beta	gnl|CDD|184438	PRK13985, ureB, urease subunit alpha.	0
NZ_CP024950.1|WP_053577752.1|1508263_1510111_-|arginine-decarboxylase	gnl|CDD|224088	COG1166, SpeA, Arginine decarboxylase (spermidine biosynthesis) [Amino acid transport and metabolism].	0
NZ_CP024950.1|WP_145940665.1|1502180_1503890_+|DUF262-domain-containing-protein	gnl|CDD|224396	COG1479, COG1479, Uncharacterized conserved protein [Function unknown].	8.38257e-44
NZ_CP024950.1|WP_000901271.1|1486847_1487435_+|acid-activated-urea-channel	gnl|CDD|259831	cd13404, UreI_AmiS_like, UreI/AmiS family, proton-gated urea channel and putative amide transporters. This family includes UreI proton-gated urea channels as well as putative amide transporters (AmiS of the amidase gene cluster). Helicobacter pylori UreI (HpUreI), a proton-gated inner membrane urea channel opens in acidic pH to allow urea influx to the cytoplasm. There urea is metabolized, producing NH3 and CO2, leading to buffering of the periplasm. This action is essential for the survival of H. pylori in the stomach, and has been identified as a mechanism that could be clinically targeted to prevent various illnesses associated with infection by H. pylori. UreI and the related amide channels (AmiS) appear to function as hexamers, and have 6 predicted transmembrane segments. UreI has also been shown have a lipid "plug" in the center of the hexamer. Urea enters at the periplasmic opening of UreI and must pass 2 constriction sites, one on each side of a conserved Glu (Glu 177, H. pylori numbering), to reach the cytoplasm. Urea/thiourea selectivity is diminished by mutation of a conserved Trp to Ala or Phe in constriction site 2 (cytoplasmic). Channel functionality is greatly diminished by mutation of a conserved Trp in constriction site 1 (periplasmic) and a conserved Tyr in constriction site 2, and to a lesser extent a conserved Phe in site 1. In the cytoplasm, urease hydrolyzes urea to form ammonia and carbamate, which decomposes to carbonic acid. UreI is fully open at pH 5.0 to facilitate urea influx, but closes at neutral pH, preventing over-alkalization. Glu 177 (H. pylori numbering) is present in urea channel proteins, but absent in the related amide channels, suggesting that it plays a role in urea specificity.	4.82187e-75
NZ_CP024950.1|WP_145940658.1|1487436_1487949_+|urease-accessory-protein-UreE	gnl|CDD|237322	PRK13261, ureE, urease accessory protein UreE; Provisional.	5.06093e-47
NZ_CP024950.1|WP_145940667.1|1510125_1511295_-|glycosyltransferase	gnl|CDD|340844	cd03817, GT4_UGDG-like, UDP-Glc:1,2-diacylglycerol 3-a-glucosyltransferase and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. UDP-glucose-diacylglycerol glucosyltransferase (EC 2.4.1.337, UGDG; also known as 1,2-diacylglycerol 3-glucosyltransferase) catalyzes the transfer of glucose from UDP-glucose to 1,2-diacylglycerol forming 3-D-glucosyl-1,2-diacylglycerol.	5.68455e-121
NZ_CP024950.1|WP_145940666.1|1503969_1505214_+|DHH-family-phosphoesterase	gnl|CDD|223681	COG0608, RecJ, Single-stranded DNA-specific exonuclease [DNA replication, recombination, and repair].	3.94715e-37
NZ_CP024950.1|WP_145940662.1|1492975_1493395_-|SMI1/KNR4-family-protein	gnl|CDD|378155	pfam09346, SMI1_KNR4, SMI1 / KNR4 family (SUKH-1). Proteins in this family are involved in the regulation of 1,3-beta-glucan synthase activity and cell-wall formation. Genome contextual information showed that SMI1 are primary immunity proteins in bacterial toxin systems.	3.0762e-08

Protein sequences

>NZ_CP024950.1|WP_145940662.1|1492975_1493395_-|SMI1/KNR4-family-protein
MDNIPTNTELNWEFVEPLNENALSDLEERLEIGFSDAFKDFINRSNYGFSQWRYFMVGNESYTFKHVLNFNLEGLFVDFMQSLKEWLEPEEIVFANDGYGGYYLLNTASDVVLFLDTDNGSKKALLNLNMFLKKLESRG
>NZ_CP024950.1|WP_145940661.1|1492626_1492974_-|hypothetical-protein
MFSHKVFLEGCTNELRRICDYFVENAMQDDLGQKLKSEALEEMLKIAHDLENLEQETQYEMRKINEQIEEAKSLMRQIDMNFHSPSEIDRLMREAKEHEREAKRRYDEYLKDKND
>NZ_CP024950.1|WP_145940660.1|1489352_1490150_+|urease-accessory-protein-UreD
MNTYAQESKLRLKTKIGADGRCVIEDNFFTPPFKLMAPFYPKDDLAEIMLLAVSPGLMKGDAQDMQLNIGPNCKLRITSQSFEKIHNTEDGFASRDMHIVVGENAFLDFAPFPLIPFENAHFKGNTTISLHSGSQLLYSEIIVAGRVARNELFKFNRLHTKISILQDEKPIYYDNTILDPKTTDLNNMCMFDGYTHYLNLVLVNCPIELSFVRECIEESEGVDGAVSETASSHLCVKALAKGSEPLLHLREKIARLTTQIITQKV
>NZ_CP024950.1|WP_000238762.1|1488753_1489353_+|urease-accessory-protein-UreG
MVKIGVCGPVGSGKTALIEALTRHMSKDYDMAVITNDIYTKEDAEFMCKNSVMPRERIIGVETGGCPHTAIREDASMNLEAVEEMHGRFPNLELLLIESGGDNLSATFNPELADFTIFVIDVAEGDKIPRKGGPGITRSDLLVINKIDLAPYVGADLKVMERDSKKMRGEKPFIFTNIRAKEGLDDVIAWIKRNALLED
>NZ_CP024950.1|WP_145940659.1|1487960_1488725_+|urease-accessory-protein-UreF
MDKGKSVKSTKKSVGMPLKTPKTDNNAHVDNEFLILQVNDAVFPIGSYTHSFGLETYIQQKKVTHKESALEYLKANLSSQFLYTEMLSLKLTYESTLQQDLKKILGVEEIITLSTSPMELRLANQKLGNRFIKTLQAMNELDMGTFFNAYAQQTKDPTHATSYGVFAASLGIELKKALRHYLYAQTSNMVINCVKSVPLSQNDGQKILLSLQNPFNQLIEKTLELDESHLCAASVQNDIKAMQHESLYSRLYMS
>NZ_CP024950.1|WP_145940658.1|1487436_1487949_+|urease-accessory-protein-UreE
MIIERLIGNLRDLNPLDFSVDYVDLEWFETRKKIARFKTRQGKDIAIRLKDAPKLGLSQGDILFKEEKEIIAVNILDSEVIHIQAKSVAEVAKICYEIGNRHAALYYGESQFEFKTPFEKPTLALLEKLGVQNRVLSSKLNSRERLTVSMPHSEPNFKVSLASDFKVVMK
>NZ_CP024950.1|WP_000901271.1|1486847_1487435_+|acid-activated-urea-channel
MLGLVLLYVGIVLISNGICGLTKVDPKSTAVMNFFVGGLSIVCNVVVITYSALHPTAPVEGAEDIVQVSHHLTSFYGPATGLLFGFTYLYAAINHTFGLDWRPYSWYSLFVAINAVPAAILSHYSDMLDDHKVLGITEGDWWAIIWLAWGVLWLTAFIENILKIPLGKFTPWLAIIEGILTAWIPAWLLFIQHWV
>NZ_CP024950.1|WP_000724295.1|1484946_1486656_+|urease-subunit-beta
MKKISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSNNPSKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEALAGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKWMLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKYDVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTNPTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSSDSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISEYVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAHHGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCRNITKKDMQFNDTTAHIEVNPETYHVFVDGKEVTSKPANKVSLAQLFSIF
>NZ_CP024950.1|WP_000779223.1|1484226_1484943_+|urease-subunit-alpha
MKLTPKELDKLMLHYAGELAKKRKEKGIKLNYVEAVALISAHIMEEARAGKKTAAELMQEGRTLLKPDDVMDGVASMIHEVGIEAMFPDGTKLVTVHTPIEANGKLVPGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRCLDFDREKTFGKRLDIASGTAVRFEPGEEKSVELIDIGGNRRIFGFNALVDRQADNESKKIALHRAKERGFHGAKSDDNYVKTIKE
>NZ_CP024950.1|WP_039093380.1|1483409_1483883_+|lipoprotein-signal-peptidase
MLNTTQQSLLVFIGVFFLIFGVDQAIKYAILEGFRYESLVIDIVLVFNKGVAFSLLSFLEGGLKYLQILLILGLFIFLMRQKELFKNHAIEFGMVFGAGVSNVLDRFVHGGVVDYVYYHYGFDFAIFNFADVMIDVGVGVLLLRQFFFKQKQNKIKA
>NZ_CP024950.1|WP_145940663.1|1494925_1495186_-|type-VII-secretion-protein
MSKVQMDTEEVREFVGHLERFRELLREEVNSLSNHFHNLESWQDAKRERFSEVVDNLKGTFNEFDEATQEQIAWLKERIRVLEQDY
>NZ_CP024950.1|WP_145940664.1|1495201_1495771_-|hypothetical-protein
MMTKNAYAFVVIEKSIMVFKCAKDKGLIPIFEGFVPLKEGFLRSFKERCNLEFLENLDFLFLYDYQFSSEVFSLCKDLKNSIWDRKLVVALVEALEGFKGLNLSLKIEDRYSNSLGNGIQNLLTNADLGSTHKAIVIDSMKTYHQSQQEKYKRERGETLEVLPTTPPSYGGGSIRISGDKKPDSNEENF
>NZ_CP024950.1|WP_079338282.1|1497039_1497354_-|transposase
MKSMQKLWGRKVSDIAFSEFVKILENKAHVVKIDRFYPSSKTCSNCLSVDENFNKDIKKLGKTDKEREYHCKYCGLELDRDLNASINIHRVGASTLGVEFVRPT
>NZ_CP024950.1|WP_161594004.1|1501841_1502033_+|hypothetical-protein
MAFYHSIILSFYHSIILSFYHSIILSFYHSIILSFYHSIILSFYHSSNQSNATILLSLLQKSA
>NZ_CP024950.1|WP_145940665.1|1502180_1503890_+|DUF262-domain-containing-protein
MMAKVEVELKKLYQILVDAEYFYQVPDYQRPYVWDKDHLGALIDDLVGSYTNNREDEYFCGSIVIAENPKDKRWDVVDGQQRLTSFIILACTILRLYKHRLGQKSADFIKESIYDKYDKEKERLKFLTAQNYNSIFENTVLNNLEFEDNIKKSELNKKFEENTYLRNAYYFKELLNESIKNGSISDMDDFVKWFYEHIALTRIVCFEQDSAMQIFQVLNDRGQPLSPIDILKSSLMQEIKQDSEKRKDFITTWDKLVEACKSVEGVDIDLEDFFNMYLEYADPSASKKRADKGLKKVFKDSKKDACEFIYGISEFMKSYTDLLKKQDRYIYLLRYLPSRYWASILTTALYVKYPDFDALKKLLVSYYYQTWIAGGTITRIKQTSINIIKNVKSNKDIETIKELILDNIESYNTFNQYHYNLWKSSSVYPSKWVRPVLALANYFMADEEKPYFIVMDAETQVEHILPQKPKRGSQWNADFDKEKREEWVNNIANLTLLKRKKNAQALNGDFDEKRKIYGGKDTSKVISCYDITKELYSSYRKWNEKSLQERHDFLYNKITPVLHIEGQEE
>NZ_CP024950.1|WP_145940666.1|1503969_1505214_+|DHH-family-phosphoesterase
MPKKELLKMSKKRIFKDFLEEVKHHRPIVFYTDNDCDGMLAGSVLMSMCYRLGIKDFFFFSPLRNAHGYGFTNLAINDLLSKPCIFNPKTNQLVRLDCIKNQFQKNPLLFSADLGADLATNTELQEILLEHFEQCIITDHHKSFEVDWIDKNKIAYINLNDEKDANYYSGAFTSALVFSQIFQIQTTPLEEELIAITLLSDRIDLDNGDNLDMILNLVQPKHDRIQCFFKDKDLSLAQDDSDEISNLYGFNCINYINALSRLSGAREFKGCYDSYLHYLVLKHFNPISDPRLSVFNVKEFKRYNDIKKKMVKESEENAQIFSCNKILVAILDESCSIKVGVSGLVANNFLKKYPSNRSHRDNKNGYSGSARGGGNFLSQIKTIPLIQAGGHEEAFGLSFAKEDFKKVIKSLQAL
>NZ_CP024950.1|WP_145940101.1|1507239_1508130_+|hypothetical-protein
MGFQNENKLKVGASVKATINDKVVEAKVISIGFNRVTLRSEKGNEATYAFNSDKFLKWFKEVPLNEVSHNHTETSADDLLKGLKIVTSGPSVKERTSTPKEPKESRFKLDFEFFGKDEETSGFLSVASLYANTERKRRLSSLFVPMVFEGQGFQASMIIIHALTCASKLKRHSDAEWYQMIKTRNADECYWDTFDDMDREDIRLFCNLIVAYANDEKGEITLNDPFAKVLPKDEKEAMFVAQLLCDGGINKYDLSCGGLTASLLDDNLGYYGLLTNSDLTNENEGEAENTTFKEEE
>NZ_CP024950.1|WP_053577752.1|1508263_1510111_-|arginine-decarboxylase
MQEVHDYGINFWSNNEFKIEKGLVKVCHGKNPSLLEIVQSVRDKGYRGPLLVRFPHLVQKQIKSLFDAFSSAIKEYQYSGAFKAVFPLKVNQMPSFVFPLVQGAKGLDYGLEAGSKSELIIAMSYTNPKAPITVNGFKDKEMIELGFIAKSMQHEITLTIEGLNELKTIIAVAKQNDFVACPKIGIRIRLHSAGTGVWAKSGGINSKFGLSSTEVLEAIRLLEENDLLEHFHMIHFHIGSQISDISPLKKALREAGNLYAELRKMGAKNLNSVNIGGGLAVEYTQHKHHQDKNYTLEEFSADVVFLLREIVKNKQEIEPDIFIESGRYISANHAVLVAPVLELFSHEYNEKSLKIKENNNPPLIDEMLDLLANINEKNAIEYLHDSFDHTESLFTLFDLGYIDLIDRSNTEVLAHLIVKKAVQLLYVKDHNDILRIQEQVQERYLLNCSFFQSLPDYWGLRQNFPVMPLNKLDEKPTRSASLWDITCDSDGEIAFDSTKPLFLHDIDIDEEEYFLAFFLVGAYQEVLGMKHNLFTHPTEFSVVFDEKGDYEVEDICEAQTILDVLDDLDYDTKEIERLLKQKIEDNNQLDMEEKKEIMGRLYVMLSENGYLRTIS
>NZ_CP024950.1|WP_145940667.1|1510125_1511295_-|glycosyltransferase
MVIVLVVDSFKDTSNGTSMTAFRFFEALKKRGHVMRVVAPHVDNLGSEEEGYYNLKEHYIPLVTEISHKQHILFAKPDEKILRKAFKGADMIHTYLPFLLEKTAVKIAREMQVPYIGSFHLQPEHISYNMKLGWFSWFNMMLFSWFKSSHYRYIHHIHCPSKFIVEELEKYNYGGKKYAISNGFDPMFRFEHPQKSLFDTTPFKIAMVGRYSNEKNQSVLIKAVALSRYKQDIVLLLKGKGPDEKKIKLLAQKLGVKTEFGFVNSNELLEILKTCTLYVHTANVESEAIACLEAISVGVVPVIANSPLSATRQFALDERSLFEPNNAKDLSAKIDWWLENKLERERMQNEYAKSALNYTLENSVIQIEKVYEEAIRDFKNNPHLFKTLS
>NZ_CP024950.1|WP_001158316.1|1511299_1511728_-|PaaI-family-thioesterase
MQESVVRVDYDSLETCKNFKPSVGTELVVLEKDIAHARFKGNESMVYEENFVHAGFVLIACNYAALCALNKRHSVVVSNNINFYAPLELNQEALIKAQVIQDGVKKAEIKIEAFVLDIQVLEGMIEIVVFDKKPFKFNFKEE

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP024950_1	1.1|577950|28|NZ_CP024950|CRISPRCasFinder	577950-577977	28	NZ_CP045351	Vibrio sp. THAF100 plasmid pTHAF100_a, complete sequence	1090166-1090193	5	0.821
NZ_CP024950_1	1.1|577950|28|NZ_CP024950|CRISPRCasFinder	577950-577977	28	KJ101592	Enterobacter phage PG7, complete genome	168930-168957	6	0.786
NZ_CP024950_1	1.1|577950|28|NZ_CP024950|CRISPRCasFinder	577950-577977	28	GU323318	Enterobacteria phage CC31, complete genome	161397-161424	6	0.786
NZ_CP024950_1	1.1|577950|28|NZ_CP024950|CRISPRCasFinder	577950-577977	28	LT614807	Cronobacter phage Pet-CM3-4 genome assembly, chromosome: I	167672-167699	6	0.786
NZ_CP024950_1	1.2|578004|31|NZ_CP024950|CRISPRCasFinder	578004-578034	31	MK448589	Streptococcus satellite phage Javan632, complete genome	4591-4621	6	0.806
NZ_CP024950_1	1.2|578004|31|NZ_CP024950|CRISPRCasFinder	578004-578034	31	NZ_CP022523	Pseudoalteromonas sp. NC201 plasmid pNC201, complete sequence	752262-752292	8	0.742

1. spacer 1.1|577950|28|NZ_CP024950|CRISPRCasFinder matches to NZ_CP045351 (Vibrio sp. THAF100 plasmid pTHAF100_a, complete sequence) position: , mismatch: 5, identity: 0.821

gtgtcgttattaaagctagtattgtcat	CRISPR spacer
gtgtcgttactaaagctaatattcttac	Protospacer
*********.********.**** *.*.

2. spacer 1.1|577950|28|NZ_CP024950|CRISPRCasFinder matches to KJ101592 (Enterobacter phage PG7, complete genome) position: , mismatch: 6, identity: 0.786

gtgtcgttattaaagctagtattgtcat	CRISPR spacer
aagaagttattgaaggtagtattgtcat	Protospacer
. *  ******.*** ************

3. spacer 1.1|577950|28|NZ_CP024950|CRISPRCasFinder matches to GU323318 (Enterobacteria phage CC31, complete genome) position: , mismatch: 6, identity: 0.786

gtgtcgttattaaagctagtattgtcat	CRISPR spacer
aagaagttattgaaggtagtattgtcat	Protospacer
. *  ******.*** ************

4. spacer 1.1|577950|28|NZ_CP024950|CRISPRCasFinder matches to LT614807 (Cronobacter phage Pet-CM3-4 genome assembly, chromosome: I) position: , mismatch: 6, identity: 0.786

gtgtcgttattaaagctagtattgtcat	CRISPR spacer
aagaagttattgaaggtagtattgtcat	Protospacer
. *  ******.*** ************

5. spacer 1.2|578004|31|NZ_CP024950|CRISPRCasFinder matches to MK448589 (Streptococcus satellite phage Javan632, complete genome) position: , mismatch: 6, identity: 0.806

ttgctgttttcaaaagcagattgggtgctat-	CRISPR spacer
ttgctgttttcaaaaggcgatt-ggtacggtt	Protospacer
****************  **** ***.* .* 

6. spacer 1.2|578004|31|NZ_CP024950|CRISPRCasFinder matches to NZ_CP022523 (Pseudoalteromonas sp. NC201 plasmid pNC201, complete sequence) position: , mismatch: 8, identity: 0.742

ttgctgttttcaaaagcagattgggtgctat	CRISPR spacer
ttgctgttttcaaaaggagattgccttgatc	Protospacer
**************** ******  *    .