CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Prophage number
NZ_AP019636	Enterobacter sp. 18A13 plasmid pECC18A13-2, complete sequence	0 crisprs	NA	0
NZ_AP019635	Enterobacter sp. 18A13 plasmid pECC18A13-1, complete sequence	0 crisprs	RT	2
NZ_AP019634	Enterobacter sp. 18A13	0 crisprs	cas3,DEDDh,csa3,WYL,DinG	5

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

20024 : 32957

Stx2-converting_phage(37.5%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_172621944.1\|20024_21252_+\|transposase	IS3 family transposase	Q9ZXG3	Shigella_phage	1.7e-145	81.7
WP_172621907.1\|22188_23529_-\|transposase	IS110 family transposase	Q9JMN8	Wolbachia_phage	1.8e-116	47.7
WP_143345840.1\|25198_25477_+	hypothetical protein	A0A0U2QV73	Escherichia_phage	3.9e-13	43.3
WP_143345773.1\|25842_26337_+	conjugal transfer protein	NA	NA	NA	NA
WP_143345774.1\|26543_27173_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_143345775.1\|28985_30557_-\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	6.0e-175	60.4
WP_143345776.1\|30576_30924_-	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	2.4e-44	72.0
WP_143345777.1\|30920_31613_-\|transposase	transposase	A0A0P0ZBP6	Stx2-converting_phage	1.5e-16	43.6
WP_164472294.1\|31611_32118_+\|transposase	transposase	A0A1B1P776	Bacillus_phage	2.1e-12	32.5
WP_164472293.1\|32093_32957_+\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	1.6e-76	51.7

DBSCAN-SWA_2

71357 : 138923

Stx2-converting_phage(33.33%)

integrase,transposase

attL 61801:61860|attR 146625:147441

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
61801:61860	attL	NA	NA	NA	NA
WP_172621949.1\|71357_71816_+\|transposase	IS3 family transposase	Q9ZXG3	Shigella_phage	3.9e-26	47.7
WP_123061052.1\|71815_72154_+\|integrase,transposase	DDE-type integrase/transposase/recombinase	A0A1B0Z042	Pseudomonas_phage	9.0e-28	58.6
WP_123061053.1\|72202_73105_+	LysR family transcriptional regulator	NA	NA	NA	NA
WP_123061085.1\|73197_73356_+	hypothetical protein	NA	NA	NA	NA
WP_143345799.1\|73593_74709_+\|transposase	IS110 family transposase	NA	NA	NA	NA
WP_143345844.1\|75435_76872_+	group II intron reverse transcriptase/maturase	A0A0N7AE80	Bacillus_phage	4.7e-09	23.2
WP_172621950.1\|76947_77175_-	hypothetical protein	NA	NA	NA	NA
WP_143345802.1\|77937_78702_-	SDR family oxidoreductase	Q06VL0	Trichoplusia_ni_ascovirus	2.6e-14	31.1
WP_143345803.1\|81606_83286_+	ShlB/FhaC/HecB family hemolysin secretion/activation protein	NA	NA	NA	NA
WP_143345804.1\|83296_83848_+	toxin-activating lysine-acyltransferase	NA	NA	NA	NA
WP_143345805.1\|83900_84476_+	Ail/Lom family outer membrane beta-barrel protein	NA	NA	NA	NA
WP_143345806.1\|84605_93572_+	hemagglutinin repeat-containing protein	A0A0R6PJK4	Moraxella_phage	3.3e-52	39.0
WP_143345807.1\|93568_93817_+	hypothetical protein	NA	NA	NA	NA
WP_143345808.1\|93902_94397_+	DUF2569 domain-containing protein	NA	NA	NA	NA
WP_172621957.1\|94406_95072_+	hypothetical protein	NA	NA	NA	NA
WP_172621958.1\|95383_95998_+	hypothetical protein	NA	NA	NA	NA
WP_143345809.1\|95994_96252_+	hypothetical protein	NA	NA	NA	NA
WP_143345810.1\|96350_96935_+	adhesin	NA	NA	NA	NA
WP_143345811.1\|96931_97183_+	hypothetical protein	NA	NA	NA	NA
WP_123061013.1\|97619_98000_+	relaxosome protein TraM	NA	NA	NA	NA
WP_172621951.1\|98052_98574_-	response regulator	A0A1V0SGX0	Hokovirus	8.7e-06	29.7
WP_143345813.1\|98728_99091_-	nuclear transport factor 2 family protein	NA	NA	NA	NA
WP_143345814.1\|99198_100110_+	LysR family transcriptional regulator	NA	NA	NA	NA
WP_143345815.1\|100017_100887_-	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	8.2e-49	95.8
WP_143345847.1\|100926_101289_+	hypothetical protein	NA	NA	NA	NA
WP_172621952.1\|101967_103186_+\|transposase	IS3 family transposase	Q6H9S6	Enterobacteria_phage	2.9e-137	84.1
WP_123061055.1\|103783_104356_+	cytochrome b/b6 domain-containing protein	NA	NA	NA	NA
WP_123061056.1\|104407_104989_+	DUF2913 family protein	NA	NA	NA	NA
WP_143345818.1\|105126_105366_+	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	5.4e-19	66.1
WP_123061058.1\|105385_105649_+	hypothetical protein	A0A0P0ZEB3	Stx2-converting_phage	2.2e-05	45.5
WP_123061086.1\|105645_105843_+	hypothetical protein	NA	NA	NA	NA
WP_143345848.1\|105847_106216_+\|transposase	transposase	A0A0P0ZEB3	Stx2-converting_phage	1.1e-23	46.5
WP_172621953.1\|106938_107913_-	ParB/RepB/Spo0J family partition protein	A0A1B0V750	Salmonella_phage	1.5e-83	53.2
WP_123061061.1\|107912_109118_-	AAA family ATPase	A0A077SL49	Escherichia_phage	1.8e-163	69.6
WP_123061062.1\|109357_109822_+	GyrI-like domain-containing protein	NA	NA	NA	NA
WP_123060990.1\|110527_110800_+	hypothetical protein	NA	NA	NA	NA
WP_143345820.1\|110826_113943_+	inverse autotransporter beta domain-containing protein	NA	NA	NA	NA
WP_172621954.1\|114037_114205_+	Ig-like domain-containing protein	NA	NA	NA	NA
WP_143345822.1\|114201_115221_+	Ig-like domain-containing protein	NA	NA	NA	NA
WP_143345823.1\|115908_116331_+	hypothetical protein	K7P834	Enterobacteria_phage	8.0e-26	47.2
WP_143345824.1\|116469_117120_+	YceH family protein	NA	NA	NA	NA
WP_123061088.1\|117122_118046_+	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_123061066.1\|118158_118449_-	Ail/Lom family outer membrane beta-barrel protein	NA	NA	NA	NA
WP_143345825.1\|119115_119448_+	IS66 family insertion sequence element accessory protein TnpB	NA	NA	NA	NA
WP_143345826.1\|119434_119815_+	IS66 family insertion sequence element accessory protein TnpB	NA	NA	NA	NA
WP_143345827.1\|120750_121239_-	hypothetical protein	NA	NA	NA	NA
WP_143345828.1\|123856_124843_-\|integrase	tyrosine-type recombinase/integrase	A0A1P8DJ76	Virus_Rctr85	1.0e-47	39.4
WP_123061072.1\|126302_127133_+	glycosyltransferase	NA	NA	NA	NA
WP_143345829.1\|127144_128569_+	oligosaccharide flippase family protein	NA	NA	NA	NA
WP_143345774.1\|130790_131420_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_164472294.1\|131720_132227_+\|transposase	transposase	A0A1B1P776	Bacillus_phage	2.1e-12	32.5
WP_164472293.1\|132202_133066_+\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	1.6e-76	51.7
WP_143345831.1\|133113_133737_+\|transposase	transposase	A0A0P0ZBP6	Stx2-converting_phage	1.3e-16	43.6
WP_143345776.1\|133733_134081_+	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	2.4e-44	72.0
WP_143345775.1\|134100_135672_+\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	6.0e-175	60.4
WP_143345832.1\|135882_136455_-	NAD(P)H-dependent oxidoreductase	NA	NA	NA	NA
WP_143345833.1\|137204_137843_-	glutathione S-transferase family protein	NA	NA	NA	NA
WP_143345834.1\|138191_138923_-	3-oxoacyl-ACP reductase FabG	Q06VL0	Trichoplusia_ni_ascovirus	3.2e-22	33.5
146625:147441	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_AP019634

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

89920 : 99132

Enterobacteria_phage(100.0%)

integrase,capsid

attL 87586:87608|attR 99293:99315

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
87586:87608	attL	NA	NA	NA	NA
WP_143344473.1\|89920_92254_-	toprim domain-containing protein	Q7M2A8	Enterobacteria_phage	0.0e+00	85.3
WP_143344474.1\|92268_92589_-	DUF5375 domain-containing protein	NA	NA	NA	NA
WP_032983269.1\|92585_92813_-	hypothetical protein	NA	NA	NA	NA
WP_143344475.1\|92809_93367_-	host cell division inhibitor Icd-like protein	Q7M2A7	Enterobacteria_phage	3.5e-29	64.8
WP_172621897.1\|93363_93630_-	AlpA family transcriptional regulator	Q7M299	Enterobacteria_phage	2.9e-29	70.5
WP_143344476.1\|94171_94915_+\|capsid	capsid protein	NA	NA	NA	NA
WP_014830132.1\|94917_95136_+	ogr/Delta-like zinc finger family protein	Q7M294	Enterobacteria_phage	1.9e-15	62.1
WP_062676834.1\|95164_95728_+	Polarity suppression protein	Q7M2A1	Enterobacteria_phage	6.0e-61	65.8
WP_143344477.1\|96576_96810_-	hypothetical protein	NA	NA	NA	NA
WP_143344478.1\|96954_97953_-	GAF domain-containing protein	NA	NA	NA	NA
WP_143344479.1\|97962_99132_-\|integrase	tyrosine-type recombinase/integrase	Q7M297	Enterobacteria_phage	2.5e-210	92.5
99293:99315	attR	NA	NA	NA	NA

DBSCAN-SWA_2

917497 : 971688

Salmonella_phage(30.3%)

terminase,holin,coat,integrase,capsid

attL 921365:921410|attR 970985:971030

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_111965853.1\|917497_918550_-	phosphoporin PhoE	Q1MVN1	Enterobacteria_phage	1.3e-117	60.1
WP_021242883.1\|918854_919958_+	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	1.7e-59	39.7
WP_127729690.1\|919969_921223_+	glutamate-5-semialdehyde dehydrogenase	A0A1X9I5D4	Streptococcus_phage	2.3e-97	46.6
921365:921410	attL	NA	NA	NA	NA
WP_143344669.1\|921424_922588_-\|integrase	site-specific integrase	A0A220NQU7	Salmonella_phage	5.3e-221	95.6
WP_143344671.1\|922818_923010_-	hypothetical protein	A0A1B0V7L5	Salmonella_phage	3.8e-07	62.5
WP_143344672.1\|923045_923420_-	hypothetical protein	R9TNF9	Aeromonas_phage	7.1e-10	36.4
WP_143344673.1\|923598_923799_-	hypothetical protein	G8C7S1	Escherichia_phage	1.2e-19	66.7
WP_143344674.1\|923945_924308_-	hypothetical protein	A0A2K9VAS2	Klebsiella_virus	6.2e-43	69.7
WP_143344675.1\|924309_924903_-	DUF551 domain-containing protein	A0A2I6PID9	Escherichia_phage	7.4e-09	43.9
WP_143344676.1\|924899_925322_-	hypothetical protein	A0A1B0V865	Salmonella_phage	2.3e-25	55.8
WP_143344677.1\|925593_925941_-	DUF551 domain-containing protein	K7PH61	Enterobacteria_phage	2.2e-21	61.8
WP_143344678.1\|926419_926602_-	DUF2737 family protein	A0A220NQV6	Salmonella_phage	2.4e-11	64.8
WP_143344680.1\|926910_927393_-	siphovirus Gp157 family protein	K7P6T5	Enterobacteria_phage	2.9e-64	78.8
WP_143344681.1\|927376_928279_-	recombinase RecT	K7P7A0	Enterobacteria_phage	6.3e-137	81.5
WP_143344682.1\|928275_928584_-	hypothetical protein	K7PJM4	Enterobacteria_phage	5.0e-25	52.9
WP_143345704.1\|928665_928890_-	hypothetical protein	NA	NA	NA	NA
WP_172621903.1\|929105_929276_-	hypothetical protein	A0A192Y6R1	Salmonella_phage	2.1e-17	78.6
WP_172621904.1\|929479_929656_-	hypothetical protein	A0A1D7XFI0	Escherichia_phage	4.2e-13	58.9
WP_143344683.1\|929655_930006_-	hypothetical protein	A0A0H5AWA3	Pseudomonas_phage	1.4e-15	53.1
WP_143345705.1\|930839_931532_-	helix-turn-helix transcriptional regulator	A0A2D1GM27	Escherichia_phage	4.7e-124	92.2
WP_143344685.1\|931678_931909_+	helix-turn-helix domain-containing protein	A0A2D1GLN0	Escherichia_phage	5.7e-34	92.1
WP_063428028.1\|932019_932310_+	hypothetical protein	I6RSP4	Salmonella_phage	1.8e-37	84.4
WP_045408365.1\|932354_932513_+	hypothetical protein	Q76H53	Enterobacteria_phage	1.9e-17	88.2
WP_172621929.1\|932532_933666_+	replication protein	E5AGE9	Erwinia_phage	2.6e-63	54.1
WP_143344686.1\|933662_935030_+	replicative DNA helicase	E5AGF0	Erwinia_phage	2.7e-171	68.0
WP_143344687.1\|935332_935524_+	hypothetical protein	Q716C9	Shigella_phage	8.1e-18	66.7
WP_143345706.1\|935754_936072_+	XRE family transcriptional regulator	A0A0D5BHH6	Escherichia_phage	3.0e-25	57.9
WP_063957882.1\|936092_936287_+	hypothetical protein	NA	NA	NA	NA
WP_143344688.1\|936298_936592_+	hypothetical protein	A0A220NQX3	Salmonella_phage	5.4e-05	36.1
WP_143344689.1\|936594_936795_+	hypothetical protein	A0A2H4FUS3	Salmonella_phage	3.0e-23	85.7
WP_143344690.1\|936797_937220_+	recombination protein NinB	A0A2H4FNF5	Salmonella_phage	1.7e-71	90.0
WP_143344692.1\|937419_937647_+	hypothetical protein	NA	NA	NA	NA
WP_143344693.1\|937648_938023_+	DUF2591 family protein	A0A2D1GLI3	Escherichia_phage	1.6e-14	39.7
WP_143344694.1\|938015_938204_+	NinF family protein	NA	NA	NA	NA
WP_143344695.1\|938196_938793_+	recombination protein NinG	A0A0P0ZG59	Escherichia_phage	7.1e-36	41.8
WP_143344696.1\|938789_939344_+	phage N-6-adenine-methyltransferase	E5AGF8	Erwinia_phage	6.5e-60	59.8
WP_143344697.1\|939340_939607_+	hypothetical protein	G0ZNC5	Cronobacter_phage	7.8e-27	70.2
WP_143344698.1\|939594_939780_+	hypothetical protein	C6ZR61	Salmonella_phage	9.6e-08	51.9
WP_143344699.1\|939776_940388_+	hypothetical protein	A0A1V0E5R2	Salmonella_phage	1.8e-111	96.6
WP_143344700.1\|940966_941293_+	hypothetical protein	NA	NA	NA	NA
WP_143344701.1\|941274_941619_+\|holin	phage holin, lambda family	A0A0M3ULK9	Salmonella_phage	1.5e-25	51.4
WP_143344702.1\|941611_942001_+	M15 family metallopeptidase	A0A060DAL8	Salmonella_phage	1.4e-56	79.8
WP_143344703.1\|942170_942668_+	KilA-N domain-containing protein	A0A220NRM9	Escherichia_phage	1.3e-91	96.4
WP_143344704.1\|942835_943477_+	hypothetical protein	C4MZS1	Enterobacteria_phage	2.0e-07	52.0
WP_143344705.1\|943478_943784_+	hypothetical protein	NA	NA	NA	NA
WP_143344706.1\|943786_947869_+	SGNH/GDSL hydrolase family protein	A0A223LJ40	Erwinia_phage	6.4e-120	51.1
WP_143344707.1\|947909_948116_+	hypothetical protein	M4Q0Z5	Dunaliella_viridis_virus	3.1e-07	37.5
WP_143344708.1\|948293_948839_+	KilA-N domain-containing protein	B8K1H1	Salmonella_phage	3.6e-63	70.8
WP_143344709.1\|949017_949248_+	hypothetical protein	NA	NA	NA	NA
WP_143344710.1\|949270_949477_+	hypothetical protein	NA	NA	NA	NA
WP_143344711.1\|949480_949999_+	hypothetical protein	A0A2H4J2I6	uncultured_Caudovirales_phage	5.0e-46	58.2
WP_143344712.1\|949998_951471_+\|terminase	terminase	A0A1W6JNY3	Morganella_phage	4.3e-252	83.1
WP_143344713.1\|951483_952953_+	DUF1073 domain-containing protein	F1C5D7	Cronobacter_phage	1.7e-147	56.8
WP_143344714.1\|952879_953881_+\|capsid	minor capsid protein	F1C5D8	Cronobacter_phage	2.1e-112	67.8
WP_143344715.1\|953898_955269_+	DUF2213 domain-containing protein	F1C5D9	Cronobacter_phage	2.1e-123	52.3
WP_143344716.1\|955268_955730_+	hypothetical protein	B1GS72	Salmonella_phage	3.4e-30	51.7
WP_143344717.1\|955726_956782_+\|coat	phage coat protein	A0A1W6DYD5	Salmonella_phage	1.3e-101	53.5
WP_143344718.1\|956814_957120_+	hypothetical protein	NA	NA	NA	NA
WP_143344719.1\|957122_957503_+	hypothetical protein	F1C5E2	Cronobacter_phage	4.8e-30	56.1
WP_014883997.1\|957502_957676_+	hypothetical protein	I6R0P9	Salmonella_phage	5.1e-11	48.2
WP_006808949.1\|957675_958026_+	hypothetical protein	G0ZNE2	Cronobacter_phage	8.9e-39	67.0
WP_014883994.1\|958028_958397_+	hypothetical protein	F1C5E3	Cronobacter_phage	3.0e-45	74.6
WP_016245419.1\|958393_958777_+	hypothetical protein	F1C5E4	Cronobacter_phage	1.3e-38	58.3
WP_143344720.1\|958835_959591_+	Ig domain-containing protein	G0ZNE6	Cronobacter_phage	2.6e-51	48.2
WP_143344721.1\|959641_960385_+	hypothetical protein	G0ZNE7	Cronobacter_phage	2.2e-63	52.2
WP_143344722.1\|960457_960832_+	hypothetical protein	A0A0M4QWT0	Salmonella_phage	1.3e-24	52.4
WP_143344723.1\|960888_963228_+	tape measure protein	A0A1B1W284	Salmonella_phage	1.7e-133	49.8
WP_143344724.1\|963229_963724_+	hypothetical protein	F1C5F0	Cronobacter_phage	2.1e-57	64.6
WP_143344725.1\|963723_964194_+	hypothetical protein	F1C5F1	Cronobacter_phage	1.3e-45	56.5
WP_143344726.1\|964186_964624_+	C40 family peptidase	F1C5F2	Cronobacter_phage	3.6e-45	62.6
WP_143344727.1\|964565_967049_+	MoaD/ThiS family protein	F1C5A7	Cronobacter_phage	4.3e-284	59.6
WP_143344728.1\|967105_969079_+	SGNH/GDSL hydrolase family protein	B1GS50	Salmonella_phage	2.1e-60	77.9
WP_143344729.1\|969140_970358_+	SGNH/GDSL hydrolase family protein	A0A1P8L663	Pectobacterium_phage	3.1e-54	33.5
WP_143344730.1\|970475_970862_+	LexA family transcriptional regulator	O64339	Escherichia_phage	6.8e-56	82.8
WP_172621905.1\|971370_971688_+	hypothetical protein	Q7M297	Enterobacteria_phage	1.3e-12	39.6
970985:971030	attR	NA	NA	NA	NA

DBSCAN-SWA_3

1794994 : 1852415

Enterobacterial_phage(42.31%)

terminase,holin,head,tail,integrase,tRNA,protease,portal,capsid

attL 1805102:1805116|attR 1821633:1821647

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_021241033.1\|1794994_1796107_-\|tRNA	tRNA 2-thiouridine(34) synthase MnmA	NA	NA	NA	NA
WP_133295416.1\|1796147_1796621_-	NUDIX hydrolase	NA	NA	NA	NA
WP_143344943.1\|1796630_1797284_-	23S rRNA pseudouridine(2457) synthase RluE	NA	NA	NA	NA
WP_023292761.1\|1797401_1798652_+	NADP-dependent isocitrate dehydrogenase	Q77Z09	Phage_21	7.9e-21	94.4
WP_133294182.1\|1798729_1799077_+	DUF1304 domain-containing protein	NA	NA	NA	NA
WP_111965567.1\|1799151_1799397_+	YmjA family protein	NA	NA	NA	NA
WP_143344944.1\|1799685_1801185_-	cyclic diguanylate phosphodiesterase	NA	NA	NA	NA
WP_143344945.1\|1801420_1802941_+	cyclic diguanylate phosphodiesterase	NA	NA	NA	NA
WP_143345714.1\|1803066_1804539_+	GGDEF domain-containing protein	G3MA91	Bacillus_virus	4.5e-15	30.8
WP_008500746.1\|1804824_1805073_+	GlsB/YeaQ/YmgE family stress response membrane protein	NA	NA	NA	NA
1805102:1805116	attL	NA	NA	NA	NA
WP_029739841.1\|1805204_1805303_-	YoaK family small membrane protein	NA	NA	NA	NA
WP_143345715.1\|1805348_1806377_-	sensor domain-containing diguanylate cyclase	G3MA91	Bacillus_virus	9.1e-15	32.6
WP_143344946.1\|1806686_1806941_+	DUF333 domain-containing protein	NA	NA	NA	NA
WP_045401563.1\|1807021_1807327_+	periplasmic protein	NA	NA	NA	NA
WP_133294186.1\|1807327_1807672_-	DUF488 domain-containing protein	NA	NA	NA	NA
WP_133294187.1\|1807773_1808481_+	CTP synthase	NA	NA	NA	NA
WP_143344947.1\|1808512_1809700_-	CynX/NimT family MFS transporter	NA	NA	NA	NA
WP_172621932.1\|1809796_1810591_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_024908579.1\|1810574_1811021_-	DUF441 domain-containing protein	NA	NA	NA	NA
WP_010430055.1\|1811165_1811270_+	hypothetical protein	NA	NA	NA	NA
WP_133294189.1\|1811290_1811791_-\|tRNA	YbaK/prolyl-tRNA synthetase associated domain-containing protein	NA	NA	NA	NA
WP_143344949.1\|1812053_1813196_-\|integrase	phage integrase Arm DNA-binding domain-containing protein	O21929	Phage_21	5.4e-93	48.1
WP_008500733.1\|1813170_1813434_-	hypothetical protein	NA	NA	NA	NA
WP_046091666.1\|1813725_1814031_-	ASCH domain-containing protein	A0A0F6R7P5	Escherichia_coli_O157_typing_phage	1.7e-33	69.0
WP_143344951.1\|1814023_1814368_-	hypothetical protein	NA	NA	NA	NA
WP_172621911.1\|1814824_1815847_-	chromosome partitioning protein ParB	K7PKM7	Enterobacterial_phage	1.9e-169	91.6
WP_172621912.1\|1815846_1816206_-	hypothetical protein	K7PJS4	Enterobacterial_phage	3.7e-48	85.7
WP_143344954.1\|1816970_1817690_-	LexA family transcriptional regulator	A0A2R2X2B0	Escherichia_phage	3.1e-78	62.9
WP_023305900.1\|1817788_1818007_+	hypothetical protein	A0A2K8HL98	Pseudomonas_phage	4.9e-11	60.3
WP_143344955.1\|1818048_1818519_+	hypothetical protein	K7PJS5	Enterobacterial_phage	3.1e-79	97.4
WP_071993458.1\|1818760_1818973_+	DUF4222 domain-containing protein	S5M7S5	Escherichia_phage	3.3e-12	63.5
WP_143344956.1\|1818929_1819856_+	conserved phage C-terminal domain-containing protein	K7PLZ7	Enterobacterial_phage	1.1e-70	57.8
WP_143344957.1\|1819852_1820347_+	hypothetical protein	NA	NA	NA	NA
WP_143344958.1\|1820346_1821006_+	DNA methyltransferase	I6PDF5	Cronobacter_phage	7.7e-100	79.5
WP_143344959.1\|1821002_1821251_+	hypothetical protein	NA	NA	NA	NA
WP_143344960.1\|1821247_1821568_+	LexA family transcriptional regulator	K7PHB4	Enterobacterial_phage	8.2e-39	74.5
WP_143344961.1\|1821564_1821954_+	RusA family crossover junction endodeoxyribonuclease	K7PKN5	Enterobacterial_phage	7.8e-68	96.9
1821633:1821647	attR	NA	NA	NA	NA
WP_143344962.1\|1821950_1822940_+	DUF968 domain-containing protein	K7PJS6	Enterobacterial_phage	1.1e-185	94.2
WP_143344963.1\|1822954_1823317_+	DUF1133 family protein	K7PGW2	Enterobacterial_phage	2.3e-58	94.2
WP_172621895.1\|1823340_1823766_-	hypothetical protein	A5LH78	Enterobacteria_phage	4.1e-46	70.7
WP_143345716.1\|1823787_1824501_-	hypothetical protein	A5LH79	Enterobacteria_phage	1.9e-120	89.9
WP_022648767.1\|1824978_1825374_+\|holin	phage holin family protein	K7PHB9	Enterobacterial_phage	9.1e-64	99.2
WP_028019551.1\|1825360_1825642_+\|holin	phage holin family protein	K7PKN9	Enterobacterial_phage	8.7e-45	97.8
WP_143344965.1\|1825641_1826268_+	glycoside hydrolase family 19 protein	K7PJS7	Enterobacterial_phage	3.9e-109	92.8
WP_143344966.1\|1826275_1826545_+	hypothetical protein	K7PGW4	Enterobacterial_phage	8.5e-05	83.1
WP_143344968.1\|1826883_1827147_-	hypothetical protein	NA	NA	NA	NA
WP_143344969.1\|1827286_1828744_+	glycosyltransferase family 2 protein	A0A220NRM5	Escherichia_phage	1.4e-266	90.5
WP_143344970.1\|1828725_1829316_+	hypothetical protein	K7P7D2	Enterobacteria_phage	1.9e-110	94.9
WP_143344971.1\|1829312_1829654_+	HNH endonuclease	K7PM05	Enterobacterial_phage	2.3e-63	97.3
WP_047362834.1\|1829653_1829857_+	hypothetical protein	A0A220NRN5	Escherichia_phage	3.6e-24	85.1
WP_143344972.1\|1830036_1830522_+\|terminase	terminase	K7PGU7	Enterobacterial_phage	3.6e-78	95.0
WP_143344973.1\|1830528_1832043_+\|terminase	terminase large subunit	F1C585	Cronobacter_phage	8.3e-283	95.2
WP_137464218.1\|1832042_1833317_+\|portal	phage portal protein	F1C584	Cronobacter_phage	9.6e-248	99.3
WP_016063561.1\|1833334_1834012_+\|head,protease	HK97 family phage prohead protease	K7PKL4	Enterobacterial_phage	2.4e-125	100.0
WP_143344974.1\|1834014_1835172_+\|capsid	phage major capsid protein	Q77WA0	Escherichia_phage	6.1e-209	97.7
WP_143344975.1\|1835204_1835531_+\|head,tail	phage head-tail connector protein	K7PGU9	Enterobacterial_phage	7.5e-56	99.1
WP_129694348.1\|1835530_1835869_+\|head	phage head closure protein	K7P7L2	Enterobacteria_phage	1.9e-57	98.2
WP_143344976.1\|1835865_1836315_+	HK97 gp10 family phage protein	K7PH84	Enterobacterial_phage	1.2e-72	96.0
WP_143344977.1\|1836311_1836659_+	DUF3168 domain-containing protein	K7P7Q9	Enterobacteria_phage	6.5e-58	96.5
WP_000202242.1\|1836713_1837184_+\|tail	phage tail protein	A0A220NRQ0	Escherichia_phage	4.4e-81	98.7
WP_143344978.1\|1837240_1837642_+\|tail	phage tail protein	K7PGV0	Enterobacterial_phage	9.5e-69	98.5
WP_000050396.1\|1837665_1837929_+	DUF4035 domain-containing protein	K7P7L5	Enterobacteria_phage	8.5e-42	98.9
WP_143344979.1\|1837963_1841245_+\|tail	phage tail tape measure protein	A0A220NRN7	Escherichia_phage	0.0e+00	95.2
WP_000963855.1\|1841247_1841586_+\|tail	phage tail protein	K7P7R1	Enterobacteria_phage	6.6e-63	100.0
WP_143344980.1\|1841582_1842341_+\|tail	phage minor tail protein L	K7P6W4	Enterobacteria_phage	1.5e-147	99.2
WP_143344981.1\|1842342_1843053_+	C40 family peptidase	K7PGV2	Enterobacterial_phage	2.7e-143	96.2
WP_143344982.1\|1843046_1843493_+	hypothetical protein	K7PLY8	Enterobacterial_phage	3.1e-76	95.3
WP_143344983.1\|1843550_1844141_+\|tail	tail assembly protein	K7PH91	Enterobacterial_phage	4.3e-102	96.9
WP_143344984.1\|1844193_1847367_+	host specificity protein J	K7P7G9	Enterobacteria_phage	0.0e+00	89.8
WP_143345717.1\|1847366_1847663_+	hypothetical protein	G8C7R5	Escherichia_phage	8.4e-38	74.5
WP_143344985.1\|1847659_1848301_+	hypothetical protein	G8C7R6	Escherichia_phage	5.2e-109	87.3
WP_143344986.1\|1848265_1848634_-	hypothetical protein	NA	NA	NA	NA
WP_143344987.1\|1848765_1849998_+\|tail	tail fiber domain-containing protein	K7P7B1	Enterobacteria_phage	1.4e-57	68.5
WP_023292714.1\|1850092_1850359_-	DinI family protein	K7PKR6	Enterobacteria_phage	2.1e-40	96.6
WP_143344988.1\|1850683_1851004_+	hypothetical protein	K7PGV5	Enterobacterial_phage	3.0e-49	86.8
WP_143344989.1\|1851131_1852415_-	YeaH/YhbH family protein	A0A140HLI1	Bacillus_phage	1.3e-10	36.3

DBSCAN-SWA_4

3239906 : 3304107

Enterobacteria_phage(25.0%)

plate,integrase,tRNA,capsid

attL 3260627:3260641|attR 3271897:3271911

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_111965254.1\|3239906_3240719_-\|tRNA	tRNA pseudouridine(38-40) synthase TruA	NA	NA	NA	NA
WP_032646629.1\|3240718_3241732_-	aspartate-semialdehyde dehydrogenase	NA	NA	NA	NA
WP_111965253.1\|3241799_3242936_-	4-phosphoerythronate dehydrogenase PdxB	A0A2R8FDS8	Brazilian_cedratvirus	5.2e-19	26.9
WP_045402671.1\|3243049_3244054_+	flagella biosynthesis regulator Flk	NA	NA	NA	NA
WP_143345353.1\|3244144_3245323_-	MFS transporter	NA	NA	NA	NA
WP_014832795.1\|3245529_3246747_-	beta-ketoacyl-ACP synthase I	NA	NA	NA	NA
WP_143345354.1\|3246905_3248903_+\|tRNA	bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC	NA	NA	NA	NA
WP_048029667.1\|3248961_3249240_-	YfcL family protein	NA	NA	NA	NA
WP_111965248.1\|3249253_3249799_-	elongation factor P hydroxylase	NA	NA	NA	NA
WP_136195504.1\|3249798_3250608_-	TSUP family transporter	NA	NA	NA	NA
WP_133294868.1\|3250607_3251432_-	penicillin-insensitive murein endopeptidase	NA	NA	NA	NA
WP_143345355.1\|3251434_3252520_-	chorismate synthase	A0A291AU41	Pandoravirus	1.5e-87	47.7
WP_008502612.1\|3252580_3253513_-	50S ribosomal protein L3 N(5)-glutamine methyltransferase	NA	NA	NA	NA
WP_008502613.1\|3253658_3254210_+	endonuclease SmrB	NA	NA	NA	NA
WP_045330962.1\|3254256_3254742_-	phosphohistidine phosphatase SixA	NA	NA	NA	NA
WP_143345356.1\|3254951_3257099_-	fatty acid oxidation complex subunit alpha FadJ	NA	NA	NA	NA
WP_133294870.1\|3257098_3258409_-	acetyl-CoA C-acyltransferase FadI	NA	NA	NA	NA
WP_023294142.1\|3258655_3258940_-	YfcZ/YiiS family protein	NA	NA	NA	NA
WP_115875916.1\|3259312_3260596_+	long-chain fatty acid transporter FadL	NA	NA	NA	NA
3260627:3260641	attL	NA	NA	NA	NA
WP_115875917.1\|3260640_3261393_-	phospholipid-binding lipoprotein MlaA	NA	NA	NA	NA
WP_133294871.1\|3261706_3262636_+	formate/nitrite transporter family protein	E7DYY8	Enterobacteria_phage	3.0e-142	86.1
WP_143345732.1\|3262946_3264113_+\|integrase	tyrosine-type recombinase/integrase	Q7M297	Enterobacteria_phage	1.3e-142	62.1
WP_143345357.1\|3264113_3266249_-	hypothetical protein	NA	NA	NA	NA
WP_143345358.1\|3266835_3267108_-	ogr/Delta-like zinc finger family protein	F1BUM8	Cronobacter_phage	1.0e-26	62.8
WP_143345359.1\|3267385_3270073_-	DUF927 domain-containing protein	A0A1W6JPG0	Morganella_phage	6.8e-110	34.5
WP_143345360.1\|3270069_3270480_-	hypothetical protein	NA	NA	NA	NA
WP_013095753.1\|3270469_3270709_-	hypothetical protein	NA	NA	NA	NA
WP_172621919.1\|3270705_3271296_-	ash family protein	Q7M2A7	Enterobacteria_phage	8.9e-23	58.1
WP_044158865.1\|3271386_3272391_-\|capsid	P2 family phage major capsid protein	F1BUM2	Cronobacter_phage	1.1e-73	45.5
3271897:3271911	attR	NA	NA	NA	NA
WP_143345362.1\|3272405_3273269_-	hypothetical protein	NA	NA	NA	NA
WP_039263275.1\|3273280_3273502_-	AlpA family phage regulatory protein	A0A1V0E8E5	Vibrio_phage	1.2e-12	53.4
WP_143345363.1\|3274111_3275443_-	type VI secretion system ImpA family N-terminal domain-containing protein	NA	NA	NA	NA
WP_143345364.1\|3275546_3276017_-	hypothetical protein	NA	NA	NA	NA
WP_143345365.1\|3276031_3276487_-\|plate	type VI secretion system baseplate subunit TssE	NA	NA	NA	NA
WP_143345366.1\|3276486_3277032_-	type VI secretion system lipoprotein TssJ	NA	NA	NA	NA
WP_143345367.1\|3277009_3278095_-\|plate	type VI secretion system baseplate subunit TssG	NA	NA	NA	NA
WP_143345368.1\|3278058_3279813_-\|plate	type VI secretion system baseplate subunit TssF	NA	NA	NA	NA
WP_143345369.1\|3279900_3280326_-	DUF1240 domain-containing protein	NA	NA	NA	NA
WP_136195513.1\|3280353_3280776_-	DUF1240 domain-containing protein	NA	NA	NA	NA
WP_101701094.1\|3280775_3282044_-	PAAR domain-containing protein	NA	NA	NA	NA
WP_136195515.1\|3282074_3283676_-	type VI secretion system protein TssA	NA	NA	NA	NA
WP_143345370.1\|3283675_3287143_-	type VI secretion protein VasK	NA	NA	NA	NA
WP_143345371.1\|3287139_3288348_-	hypothetical protein	NA	NA	NA	NA
WP_136195518.1\|3288463_3288931_-	hypothetical protein	NA	NA	NA	NA
WP_143345733.1\|3288933_3291045_-	M23 family metallopeptidase	Q5ZGC9	Flavobacterium_phage	1.8e-12	50.0
WP_143345372.1\|3291166_3291394_-	hypothetical protein	NA	NA	NA	NA
WP_143345373.1\|3291486_3291768_-	hypothetical protein	NA	NA	NA	NA
WP_136195520.1\|3291918_3292509_-	hypothetical protein	NA	NA	NA	NA
WP_143345374.1\|3294723_3297075_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	3.7e-19	32.2
WP_136195522.1\|3297071_3299726_-	type VI secretion system ATPase TssH	K4FB40	Cronobacter_phage	4.5e-98	33.7
WP_023325816.1\|3299881_3300373_-	Hcp family type VI secretion system effector	NA	NA	NA	NA
WP_136195523.1\|3300377_3302084_-	OmpA family protein	NA	NA	NA	NA
WP_136195524.1\|3302080_3302770_-	DotU family type IV/VI secretion system protein	NA	NA	NA	NA
WP_136195525.1\|3302766_3304107_-\|plate	type VI secretion system baseplate subunit TssK	NA	NA	NA	NA

DBSCAN-SWA_5

3854386 : 3897980

Bacillus_phage(50.0%)

transposase,integrase,tRNA,protease

attL 3850522:3850536|attR 3886375:3886389

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
3850522:3850536	attL	NA	NA	NA	NA
WP_021242073.1\|3854386_3854884_+\|protease	SprT family zinc-dependent metalloprotease	NA	NA	NA	NA
WP_115876242.1\|3854978_3855686_+	deoxyribonuclease I	NA	NA	NA	NA
WP_143345516.1\|3855737_3856469_+	16S rRNA (uracil(1498)-N(3))-methyltransferase	NA	NA	NA	NA
WP_047173570.1\|3856488_3857436_+	glutathione synthase	NA	NA	NA	NA
WP_020883695.1\|3857523_3858084_+	YqgE/AlgH family protein	NA	NA	NA	NA
WP_021242076.1\|3858083_3858500_+	Holliday junction resolvase RuvX	NA	NA	NA	NA
WP_111963224.1\|3858509_3859490_-	type IV pilus twitching motility protein PilT	NA	NA	NA	NA
WP_115876245.1\|3859507_3860209_+	YggS family pyridoxal phosphate-dependent enzyme	NA	NA	NA	NA
WP_111963226.1\|3860230_3860797_+	YggT family protein	NA	NA	NA	NA
WP_023309131.1\|3860793_3861090_+	YggU family protein	NA	NA	NA	NA
WP_136195092.1\|3861093_3861687_+	XTP/dITP diphosphatase	NA	NA	NA	NA
WP_133295121.1\|3861679_3862822_+	radical SAM family heme chaperone HemW	NA	NA	NA	NA
WP_008499762.1\|3862995_3863712_-	DUF2884 domain-containing protein	NA	NA	NA	NA
WP_003862421.1\|3863768_3864095_-	YggL family protein	NA	NA	NA	NA
WP_127732277.1\|3864094_3864814_-\|tRNA	tRNA (guanosine(46)-N7)-methyltransferase TrmB	NA	NA	NA	NA
WP_168708010.1\|3864958_3866011_+	A/G-specific adenine glycosylase	NA	NA	NA	NA
WP_023309138.1\|3866037_3866310_+	oxidative damage protection protein	NA	NA	NA	NA
WP_143345743.1\|3866366_3867443_+	membrane-bound lytic murein transglycosylase MltC	NA	NA	NA	NA
WP_115876247.1\|3867680_3868937_+	nucleoside permease	NA	NA	NA	NA
WP_133295124.1\|3869016_3869820_-	triphosphoribosyl-dephospho-CoA synthase CitG	NA	NA	NA	NA
WP_136195089.1\|3869797_3870337_-	citrate lyase holo-[acyl-carrier protein] synthase	NA	NA	NA	NA
WP_136195088.1\|3870339_3871857_-	citrate lyase subunit alpha	NA	NA	NA	NA
WP_143345517.1\|3871867_3872743_-	citrate (pro-3S)-lyase subunit beta	NA	NA	NA	NA
WP_008499773.1\|3872739_3873033_-	citrate lyase acyl carrier protein	NA	NA	NA	NA
WP_143345518.1\|3873049_3874072_-	[citrate (pro-3S)-lyase] ligase	NA	NA	NA	NA
WP_047173579.1\|3874085_3874949_-	fumarylacetoacetate hydrolase family protein	NA	NA	NA	NA
WP_047173580.1\|3874966_3876328_-	2-hydroxycarboxylate transporter family protein	NA	NA	NA	NA
WP_164850639.1\|3876673_3878299_+	sensor histidine kinase	NA	NA	NA	NA
WP_133295128.1\|3878288_3878981_+	response regulator	NA	NA	NA	NA
WP_143345520.1\|3879035_3881171_-	ornithine decarboxylase	NA	NA	NA	NA
WP_047173584.1\|3881352_3882066_+	DUF554 domain-containing protein	NA	NA	NA	NA
WP_123060776.1\|3882430_3883684_+\|integrase	integrase arm-type DNA-binding domain-containing protein	Q7M297	Enterobacteria_phage	9.3e-78	38.3
WP_123060777.1\|3884544_3885660_+\|transposase	IS110 family transposase	NA	NA	NA	NA
WP_123060778.1\|3889180_3889528_-	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	3.8e-42	71.0
3886375:3886389	attR	NA	NA	NA	NA
WP_164472293.1\|3890244_3891108_-\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	1.6e-76	51.7
WP_164472294.1\|3891083_3891590_-\|transposase	transposase	A0A1B1P776	Bacillus_phage	2.1e-12	32.5
WP_123060994.1\|3891787_3891922_-	hypothetical protein	NA	NA	NA	NA
WP_123060995.1\|3892082_3892307_-	hypothetical protein	NA	NA	NA	NA
WP_143345522.1\|3893300_3893939_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_172621922.1\|3896035_3896710_-	EAL domain-containing protein	NA	NA	NA	NA
WP_123060784.1\|3897788_3897980_+\|transposase	transposase	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_AP019635

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_AP019634

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection