CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP013842	Clostridium botulinum strain AM1051 plasmid pRSJ13_1, complete sequence	0 crisprs	NA	0	0	0	0
NZ_CP013841	Clostridium botulinum strain AM1051 chromosome, complete genome	1 crisprs	DEDDh,csa3,DinG,c2c9_V-U4,WYL,cas6,cas8b2,cas7,cas5,cas3,cas4,cas1,cas2,RT	0	5	4	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP013841_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP013841_1

933739-934818

Unclear

Consensus_repeat	Method
CTTTTATATTAACTATGTGATATGTAAAT	PILER-CR
CTTTTATATTAACTATGTGATATGTAAAT	CRISPRCasFinder
CTTTTATATTAACTATGTGATATGTAAAT	CRT

16 spacers

cas2,cas1,cas4,cas3,cas5,cas7,cas8b2,cas6,WYL

The CRISPR arrays of NZ_CP013841_1

>merge|NZ_CP013841|1|933739-934818|PILER-CR,CRISPRCasFinder,CRT
CTTTTATATTAACTATGTGATATGTAAATGGAAATATTATGGATTACTCATATGAAATAAATGAAGACTTTTATATTAACTATGTGATATGTAAATTAGTTTCTTTAAGGTCTTCAGGAATATTTTTAATATACTTTTATATTAACTATGTGATATGTAAATTTAACTCCTAAAGATAATCCATTTATATTCATATCTCTTTTATATTAACTATGTGATATGTAAATACTACCTATTGCTATTTTAACGAATGTATGATTTCATCTTTTATATTAACTATGTGATATGTAAATTTAATTTTATAGTTTAAAGCCATAAGCTATAAGGTAGCTTTTATATTAACTATGTGATATGTAAATAAATTATGTTTATAAATGATAAAATAGAAAATAAACCTTTTATATTAACTATGTGATATGTAAATTAGAAATAAGAAATAAGGGTGAACAAATAGTCTACGCTTTTATATTAACTATGTGATATGTAAATGGTTACTGCATATGCGAAGTGTTGCAGGTAAAAATTCTTTTATATTAACTATGTGATATGTAAATACGTAAAACTCAAGCAGGGAAAGAAGTGGATAGTGATGCTTTTATATTAACTATGTGATATGTAAATAATTATTTTACTTTTGCTCCAGGAGATAAAATTGTAACTTTTATATTAACTATGTGATATGTAAATACAAATTTTATATCCTCTGTTTCCAAAGAACATTTAGCTTTTATATTAACTATGTGATATGTAAATTATCAAATCAGAATGGGAAGTAGCTTAGATGAAACATCTTTTATATTAACTATGTGATATGTAAATTTGTATATCTTTTCGCTTAAAGCCAAAGTTTCTTTTACTTTTATATTAACTATGTGATATGTAAATATTTTAAGAGCATGACTAGTAAATATAAAAGTGTGCCTTTTATATTAACTATGTGATATGTAAATTACTTATATACAATTATTGATGTTTGCTGTACCATCTTTTATATTAACTATGTGATATGTAAATGTTTATACAATATTACCTATACTTGCAGAAAACAAAACTTTTATATTAACTATGTGGGATGTAAAT

>NZ_CP013841|1|1|933739-934752|PILER-CR
CTTTTATATTAACTATGTGATATGTAAAT	GGAAATATTATGGATTACTCATATGAAATAAATGAAGA
CTTTTATATTAACTATGTGATATGTAAAT	TAGTTTCTTTAAGGTCTTCAGGAATATTTTTAATATA
CTTTTATATTAACTATGTGATATGTAAAT	TTAACTCCTAAAGATAATCCATTTATATTCATATCT
CTTTTATATTAACTATGTGATATGTAAAT	ACTACCTATTGCTATTTTAACGAATGTATGATTTCAT
CTTTTATATTAACTATGTGATATGTAAAT	TTAATTTTATAGTTTAAAGCCATAAGCTATAAGGTAG
CTTTTATATTAACTATGTGATATGTAAAT	AAATTATGTTTATAAATGATAAAATAGAAAATAAAC
CTTTTATATTAACTATGTGATATGTAAAT	TAGAAATAAGAAATAAGGGTGAACAAATAGTCTACG
CTTTTATATTAACTATGTGATATGTAAAT	GGTTACTGCATATGCGAAGTGTTGCAGGTAAAAATT
CTTTTATATTAACTATGTGATATGTAAAT	ACGTAAAACTCAAGCAGGGAAAGAAGTGGATAGTGATG
CTTTTATATTAACTATGTGATATGTAAAT	AATTATTTTACTTTTGCTCCAGGAGATAAAATTGTAA
CTTTTATATTAACTATGTGATATGTAAAT	ACAAATTTTATATCCTCTGTTTCCAAAGAACATTTAG
CTTTTATATTAACTATGTGATATGTAAAT	TATCAAATCAGAATGGGAAGTAGCTTAGATGAAACAT
CTTTTATATTAACTATGTGATATGTAAAT	TTGTATATCTTTTCGCTTAAAGCCAAAGTTTCTTTTA
CTTTTATATTAACTATGTGATATGTAAAT	ATTTTAAGAGCATGACTAGTAAATATAAAAGTGTGC
CTTTTATATTAACTATGTGATATGTAAAT	TACTTATATACAATTATTGATGTTTGCTGTACCAT
CTTTTATATTAACTATGTGATATGTAAAT

>NZ_CP013841|1|1|933739-934818|CRISPRCasFinder
CTTTTATATTAACTATGTGATATGTAAAT	GGAAATATTATGGATTACTCATATGAAATAAATGAAGA
CTTTTATATTAACTATGTGATATGTAAAT	TAGTTTCTTTAAGGTCTTCAGGAATATTTTTAATATA
CTTTTATATTAACTATGTGATATGTAAAT	TTAACTCCTAAAGATAATCCATTTATATTCATATCT
CTTTTATATTAACTATGTGATATGTAAAT	ACTACCTATTGCTATTTTAACGAATGTATGATTTCAT
CTTTTATATTAACTATGTGATATGTAAAT	TTAATTTTATAGTTTAAAGCCATAAGCTATAAGGTAG
CTTTTATATTAACTATGTGATATGTAAAT	AAATTATGTTTATAAATGATAAAATAGAAAATAAAC
CTTTTATATTAACTATGTGATATGTAAAT	TAGAAATAAGAAATAAGGGTGAACAAATAGTCTACG
CTTTTATATTAACTATGTGATATGTAAAT	GGTTACTGCATATGCGAAGTGTTGCAGGTAAAAATT
CTTTTATATTAACTATGTGATATGTAAAT	ACGTAAAACTCAAGCAGGGAAAGAAGTGGATAGTGATG
CTTTTATATTAACTATGTGATATGTAAAT	AATTATTTTACTTTTGCTCCAGGAGATAAAATTGTAA
CTTTTATATTAACTATGTGATATGTAAAT	ACAAATTTTATATCCTCTGTTTCCAAAGAACATTTAG
CTTTTATATTAACTATGTGATATGTAAAT	TATCAAATCAGAATGGGAAGTAGCTTAGATGAAACAT
CTTTTATATTAACTATGTGATATGTAAAT	TTGTATATCTTTTCGCTTAAAGCCAAAGTTTCTTTTA
CTTTTATATTAACTATGTGATATGTAAAT	ATTTTAAGAGCATGACTAGTAAATATAAAAGTGTGC
CTTTTATATTAACTATGTGATATGTAAAT	TACTTATATACAATTATTGATGTTTGCTGTACCAT
CTTTTATATTAACTATGTGATATGTAAAT	GTTTATACAATATTACCTATACTTGCAGAAAACAAAA
CTTTTATATTAACTATGTGGGATGTAAAT

>NZ_CP013841|1|1|933739-934818|CRT
CTTTTATATTAACTATGTGATATGTAAAT	GGAAATATTATGGATTACTCATATGAAATAAATGAAGA
CTTTTATATTAACTATGTGATATGTAAAT	TAGTTTCTTTAAGGTCTTCAGGAATATTTTTAATATA
CTTTTATATTAACTATGTGATATGTAAAT	TTAACTCCTAAAGATAATCCATTTATATTCATATCT
CTTTTATATTAACTATGTGATATGTAAAT	ACTACCTATTGCTATTTTAACGAATGTATGATTTCAT
CTTTTATATTAACTATGTGATATGTAAAT	TTAATTTTATAGTTTAAAGCCATAAGCTATAAGGTAG
CTTTTATATTAACTATGTGATATGTAAAT	AAATTATGTTTATAAATGATAAAATAGAAAATAAAC
CTTTTATATTAACTATGTGATATGTAAAT	TAGAAATAAGAAATAAGGGTGAACAAATAGTCTACG
CTTTTATATTAACTATGTGATATGTAAAT	GGTTACTGCATATGCGAAGTGTTGCAGGTAAAAATT
CTTTTATATTAACTATGTGATATGTAAAT	ACGTAAAACTCAAGCAGGGAAAGAAGTGGATAGTGATG
CTTTTATATTAACTATGTGATATGTAAAT	AATTATTTTACTTTTGCTCCAGGAGATAAAATTGTAA
CTTTTATATTAACTATGTGATATGTAAAT	ACAAATTTTATATCCTCTGTTTCCAAAGAACATTTAG
CTTTTATATTAACTATGTGATATGTAAAT	TATCAAATCAGAATGGGAAGTAGCTTAGATGAAACAT
CTTTTATATTAACTATGTGATATGTAAAT	TTGTATATCTTTTCGCTTAAAGCCAAAGTTTCTTTTA
CTTTTATATTAACTATGTGATATGTAAAT	ATTTTAAGAGCATGACTAGTAAATATAAAAGTGTGC
CTTTTATATTAACTATGTGATATGTAAAT	TACTTATATACAATTATTGATGTTTGCTGTACCAT
CTTTTATATTAACTATGTGATATGTAAAT	GTTTATACAATATTACCTATACTTGCAGAAAACAAAA
CTTTTATATTAACTATGTGGGATGTAAAT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP013841.1\|WP_101527653.1\|940946_941993_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP013841.1\|WP_012704810.1\|935277_936075_+\|4-hydroxy-tetrahydrodipicolinate-reductase	unknown	unknown	gnl\|CDD\|234595
NZ_CP013841.1\|WP_101527652.1\|929497_931765_+\|CRISPR-associated-helicase/endonuclease-Cas3	cas3	COG1203_cas3_CAS-I	gnl\|CDD\|187770
NZ_CP013841.1\|WP_101527654.1\|946306_946879_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP013841.1\|WP_045905527.1\|947533_947863_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP013841.1\|WP_075143583.1\|928537_929443_+\|CRISPR-associated-protein-Cas5	cas5	cd09658_cas5_CAS-I-B	gnl\|CDD\|187789
NZ_CP013841.1\|WP_075143590.1\|944322_945993_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP013841.1\|WP_012704370.1\|936096_936936_+\|sulfurtransferase	unknown	unknown	gnl\|CDD\|225450
NZ_CP013841.1\|WP_101527649.1\|924713_925154_+\|WYL-domain-containing-protein	WYL	pfam13280_WYL_CAS-I:CAS-III	gnl\|CDD\|379100
NZ_CP013841.1\|WP_075143582.1\|927623_928529_+\|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR	cas7	cd09687_cas7_CAS-I-B	gnl\|CDD\|274215
NZ_CP013841.1\|WP_158644205.1\|946941_947358_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP013841.1\|WP_075143586.1\|932297_933278_+\|type-I-B-CRISPR-associated-endonuclease-Cas1	cas1	cd09722_cas1_CAS-I-B	gnl\|CDD\|274695
NZ_CP013841.1\|WP_101527651.1\|925924_927631_+\|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1	cas8b2	cd09665_cas8b2_CAS-I-B	gnl\|CDD\|187796
NZ_CP013841.1\|WP_049042729.1\|933284_933548_+\|CRISPR-associated-endonuclease-Cas2	cas2	cd09725_cas2_CAS-I:CAS-II:CAS-III:CAS-V	gnl\|CDD\|187856
NZ_CP013841.1\|WP_101527648.1\|924170_924689_+\|HTH-domain-containing-protein	WYL	COG2378_WYL_CAS-I:CAS-III	gnl\|CDD\|225253
NZ_CP013841.1\|WP_101527650.1\|925177_925921_+\|CRISPR-associated-endoribonuclease-Cas6	cas6	COG1583_cas6_CAS-I:CAS-III:CAS-IV	gnl\|CDD\|273848
NZ_CP013841.1\|WP_075143587.1\|937448_939149_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|284995
NZ_CP013841.1\|WP_075143585.1\|931779_932298_+\|CRISPR-associated-protein-Cas4	cas4	pfam01930_cas4_CAS-I	gnl\|CDD\|366863
NZ_CP013841.1\|WP_075143588.1\|939591_940644_+\|DUF4935-domain-containing-protein	unknown	unknown	gnl\|CDD\|374474
NZ_CP013841.1\|WP_075143591.1\|947942_949391_+\|serine-hydrolase	unknown	unknown	gnl\|CDD\|365903

Protein	Function_ID	Function_description	E-value
NZ_CP013841.1\|WP_012704810.1\|935277_936075_+\|4-hydroxy-tetrahydrodipicolinate-reductase	gnl\|CDD\|234595	PRK00048, PRK00048, dihydrodipicolinate reductase; Provisional.	1.16178e-102
NZ_CP013841.1\|WP_101527652.1\|929497_931765_+\|CRISPR-associated-helicase/endonuclease-Cas3	gnl\|CDD\|187770	cd09639, Cas3_I, CRISPR/Cas system-associated protein Cas3. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; DEAD/DEAH box helicase DNA helicase cas3'; Often but not always is fused to HD nuclease domain; signature gene for Type I.	1.27524e-60
NZ_CP013841.1\|WP_075143583.1\|928537_929443_+\|CRISPR-associated-protein-Cas5	gnl\|CDD\|187789	cd09658, Cas5_I-B, CRISPR/Cas system-associated RAMP superfamily protein Cas5. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas5 is a RAMP superfamily protein; Subunit of the Cascade complex.	1.09128e-12
NZ_CP013841.1\|WP_012704370.1\|936096_936936_+\|sulfurtransferase	gnl\|CDD\|225450	COG2897, SseA, Rhodanese-related sulfurtransferase [Inorganic ion transport and metabolism].	3.23525e-72
NZ_CP013841.1\|WP_101527649.1\|924713_925154_+\|WYL-domain-containing-protein	gnl\|CDD\|379100	pfam13280, WYL, WYL domain. WYL is a Sm-like SH3 beta-barrel fold containing domain. It is a member of the WYL-like superfamily, named for three conserved amino acids found in a subset of the superfamily. However, these residues are not strongly conserved throughout the family. Rather, the conservation pattern includes four basic residues and a position often occupied by a cysteine, which are predicted to line a ligand-binding groove typical of the Sm-like SH3 beta-barrels. A WYL domain protein (sll7009) is a negative regulator of the I-D CRISPR-Cas system in Synechocystis sp. It is predicted to be a ligand-sensing domain that could bind negatively charged ligands, such as nucleotides or nucleic acid fragments, to regulate CRISPR-Cas and other defense systems such as the abortive infection AbiG system.	1.12241e-30
NZ_CP013841.1\|WP_075143582.1\|927623_928529_+\|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR	gnl\|CDD\|274215	TIGR02585, conserved_protein, CRISPR-associated protein Cas7/Cst2/DevR, subtype I-B/TNEAP. CRISPR is a term for Clustered Regularly Interspaced Short Palidromic Repeats. A number of protein families appear only in association with these repeats and are designated Cas (CRISPR associated) proteins. This clade is a member of the DevR family (TIGR01875) and includes the DevR protein of Myxococcus xanthus, a protein whose expression appears to be regulated through a number of means, including both location and autorepression; DevR mutants are incapable of fruiting body development.	1.52393e-53
NZ_CP013841.1\|WP_075143586.1\|932297_933278_+\|type-I-B-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274695	TIGR03641, cas1_HMARI, CRISPR-associated endonuclease Cas1, subtype I-B/HMARI/TNEAP. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes Cas1 subgroup that includes Cas1 proteins of the related HMARI and TNEAP subtypes of CRISPR/Cas system.	1.6224e-167
NZ_CP013841.1\|WP_101527651.1\|925924_927631_+\|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1	gnl\|CDD\|187796	cd09665, Cas8a1_I-A, CRISPR/Cas system-associated protein Cas8a1. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Large proteins, some contain Zn-finger domain; signature gene for I-A subtype; also known as CXXC_CXXC family.	1.71901e-15
NZ_CP013841.1\|WP_049042729.1\|933284_933548_+\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|187856	cd09725, Cas2_I_II_III, CRISPR/Cas system-associated protein Cas2. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas2 is present in majority of CRISPR/Cas systems along with Cas1; RNAse specific to U-rich regions; Possesses an RRM/ferredoxin fold.	4.68218e-27
NZ_CP013841.1\|WP_101527648.1\|924170_924689_+\|HTH-domain-containing-protein	gnl\|CDD\|225253	COG2378, COG2378, Predicted transcriptional regulator [Transcription].	3.11965e-16
NZ_CP013841.1\|WP_101527650.1\|925177_925921_+\|CRISPR-associated-endoribonuclease-Cas6	gnl\|CDD\|273848	TIGR01877, CRISPR-associated_endoribonuclease_Cas6_1, CRISPR-associated endoribonuclease Cas6. CRISPR is a term for Clustered, Regularly Interspaced Short Palidromic Repeats. A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-Associated) proteins. This broadly distributed, highly divergent Cas family is now characterized as an endoribonuclease that generates guide RNAs for host defense against phage and other invaders. The family contains a C-terminal motif GXGXXXXXGXG, where the each X between two Gly is hydrophobic and the spacer XXXXX contains (usually) one Arg or Lys. The seed alignment for the current version of this model has gappy columns removed. Members of this protein family are found associated with several different CRISPR/cas system subtypes, and consequently we designate this family Cas6.	2.24653e-21
NZ_CP013841.1\|WP_075143587.1\|937448_939149_+\|hypothetical-protein	gnl\|CDD\|284995	pfam07693, KAP_NTPase, KAP family P-loop domain. The KAP (after Kidins220/ARMS and PifA) family of predicted NTPases are sporadically distributed across a wide phylogenetic range in bacteria and in animals. Many of the prokaryotic KAP NTPases are encoded in plasmids and tend to undergo disruption to form pseudogenes. A unique feature of all eukaryotic and certain bacterial KAP NTPases is the presence of two or four transmembrane helices inserted into the P-loop NTPase domain. These transmembrane helices anchor KAP NTPases in the membrane such that the P-loop domain is located on the intracellular side.	0.000959362
NZ_CP013841.1\|WP_075143585.1\|931779_932298_+\|CRISPR-associated-protein-Cas4	gnl\|CDD\|366863	pfam01930, Cas_Cas4, Domain of unknown function DUF83. This domain has no known function. The domain contains three conserved cysteines at its C-terminus.	1.8906e-54
NZ_CP013841.1\|WP_075143588.1\|939591_940644_+\|DUF4935-domain-containing-protein	gnl\|CDD\|374474	pfam16289, DUF4935, Domain of unknown function (DUF4935). This family consists of uncharacterized proteins around 350 residues in length and is mainly found in various species, such as Prevotella, Pseudomonas, Leptospira and so on. The function of this family is unknown.	4.836e-10
NZ_CP013841.1\|WP_075143591.1\|947942_949391_+\|serine-hydrolase	gnl\|CDD\|365903	pfam00144, Beta-lactamase, Beta-lactamase. This family appears to be distantly related to pfam00905 and PF00768 D-alanyl-D-alanine carboxypeptidase.	3.86867e-60

>NZ_CP013841.1|WP_049042729.1|933284_933548_+|CRISPR-associated-endonuclease-Cas2
MFVILTYDIEEKRINRVRKTLKKYLVWTQNSVFEGEITEGKLHKCLSDVNKLIDKKFDSVYVYRVKNSKNIKKDVVGIEKSFDELFL
>NZ_CP013841.1|WP_075143586.1|932297_933278_+|type-I-B-CRISPR-associated-endonuclease-Cas1
MGRDYYIFSDGRLKRKDNTIYFINSDSVKRSLPIEQVDAIHIYGEVDLNTKLLNYLTQYGLILNFYNYYGYYSGSYYPRKKNVSGFLLVDQALHYHYYDKRMYLAKSFIDSAIHHILRNLRKYDNTEIFIEKIERERNKVLSSNAIDELMGAEGRARKIYYEAFNIILKNGFELEKREKRPPKDPINALISFGNSLMYTTVLGEIYKTQLDPTISYLHEPSTKRFSLSLDLAEIFKPLIIDPIIFSMINNRMIKLEHFNIEEGICFLNNEGKAKFIKQFEKRMGTTIKHRKLNRKVSYRTFIRLECYKLIKHLIGDDIYKPLKAWW
>NZ_CP013841.1|WP_075143585.1|931779_932298_+|CRISPR-associated-protein-Cas4
MKFELEEFKTQGVKINYYYVCKRKLWLFSKGITMEDTSDRVLSGKIIHEDSYKRSKNKEMLIDDILKLDIIDKEYVSEVKITSKMPLPDKMQLLYYIFYLKQMGINKKGKINYVKEKRVEKLELTEEIEEEIKNILIDIKTIEKDNIPPKLIKLPYCKKCSYYEFCYVKEEE
>NZ_CP013841.1|WP_101527652.1|929497_931765_+|CRISPR-associated-helicase/endonuclease-Cas3
MSNLFLAKTNPRETIIEHTNLLLKGLDTIKKIYPNIEFLDWDILEIACVYHDAGKVNTKFQNKLYEKLGETKLKDDLENEKEIPHGYLSPAFLPKKQLKEKYEDDILRVLYQSIYYHHNREKLDNFDPIKLAVKQDLIKYWQDFKYDKIDKSEKLYPSYKKYLGERIDSGDKVYQNYIITKGLLNKLDYAASGHIDVEVENKDLFDKTYSYLRKGGFVPNELQQYMIDNQDKNNIIIASTGIGKTEASLFWIGNNKGFFTLPLRVSINAIYDRVKDNIGFKDVALLHSDTYSEYLKRNIDLETDFYDKTKQLSMPLTICTLDQLIDFIFKYEGYEIKLATLAYSKLIIDEIQMYSPDMIAYLIMALKYITDMGGKFSIVTATMPQIIIDFMKKEEIKFNPPVTYCKKVDNKIQLRHRVTCLDEPINIKNILKNYKNKKVLIIVNTVKKAQEIYDNINSELEDSKVNINLFHSRFIKKHRAEKENDILNFGDKDCKDTGIWITTQVVEASLDIDFDVLYTELSDVSGLFQRMGRAYRSRILDIKDSNVFVYLGGDQPTSGVGSGEHSIIDIEIFKLSKNAIKNFKDKELDEEEKMSIIENVYTKENLQKANYYKKIDETIKWVKDIKEYELEKNEARLRNIFNDSIVPQSVYEDNKEFIEEALINIKTIKKEIECKKHNISSLKAKIQLEKDKIKNLIVDIPTYEFKMVLKANRIINLVKIDKFNEIAVVAYDYSFERGLTRPKNIDDFDENIQIL
>NZ_CP013841.1|WP_075143583.1|928537_929443_+|CRISPR-associated-protein-Cas5
MKAIRLKIYQNLVNYKKPTSFQLKESYPLPPYSTVSGMIHSICGFKDYKDMNISIQGNYYSKVNDLWTRYEYKFNGKILDTYTVCNECGGTQSNKKKACNKCGSTDVNYKIKPRGDFITEKQILGGNIKNNKKLYSKNRLFNEEQKENIGIPITRGVSTAELLVDVNLLIHIRPKDENLLNIIYEGLKNPKEYISLGRREDIARVDEVKIVDIEKMDTEDESKKLEYDAYIPVDMFKNSEFVSKATIYNLNKCYEKVKIKKDVYIRQWKKVKVLHGVMNKDEINEDVEFLQDENSYPVFFA
>NZ_CP013841.1|WP_075143582.1|927623_928529_+|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR
MNKKGLTASFIFEAESANYGEGVGNVTSLKKISRGSGDSFSYISRQAIRYNIINQMEEDKTPLALDGTVVQFASNATIKDYAEIDLFGYMKTEGDIKTRSAVVRLSNAVSLESFNADLDFLTNKGLLDRYNSQGKETKGGGNIAQSEIHKSYYTYTITVDLDKVGIDENEEDDEGNILEISNEEKSQRINALLEAIKFLYRDIKGRRENLSPIFSIGGVYDIKNPFFENKLKVKNNKILVDTINGVFKLDESIEKNTVSGLIEGIFNNDEEMKTQLDCKDMGEFFNVLKEEVDRYYKDETK
>NZ_CP013841.1|WP_101527651.1|925924_927631_+|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1
MKKIANIKLELSDWLYNAGIVGICKIFEANDDYYEKHSNYIEFDESLLENFEEKYFNYFINKYEKFTSWYKIVSIEDKIVNFNKYAVDEKYMDKFNKQIDYVKDKLTSASYESGYEVIENKEMDLLKEERKLKKIKKTKKQSIEDIIPMIEEQNELLQRIIEYLKKEEVKIIIMAKNAIYDVISKFWSDVSFLNSNNNKKNMYKEYKNYFLDYCINYIHEDKEKFKYTCFSCDNKISKLSKPASYNLTWINKMGVDMSRKTSHFWNFYGDTFICPICNLIYSCIPAGFTILKDKGIFINENSNMDTLIKVNNTAIDNNISFEELEEKSYFTITDNMKQSEFERSNKEIDNIQIIKLDSNNQIKPYTFNIMSKNKLKIIFDNRKNLRKLVNISIKVGEKNKKPIYINLYSEVLKRVYNNKNLFDLISQLFYLKLNDKFNGLLYLKNIIKINNSFLGGIMGKKIYYKKIDGIQQMGFCLRKEYEDKKAGNKINGITYRLLNALKTKNSNRFMDTLLNAYMYIKKPIPTEFIEGLKDEAKLQTIGYAFLLGVQGDNFKLESNEKEGVVNNE
>NZ_CP013841.1|WP_101527650.1|925177_925921_+|CRISPR-associated-endoribonuclease-Cas6
MKTLKLSCEYKTEKIPIAYSMMFVSLIKEALKKSDEEYFKSLYMYEDIKKNKKTKDFCFSVYLKDFSKEENVFIIKDKIIFNISSPNYEFMIKLYNGLLKITNFKYKEFYINKVKINLVNDKNIENNNHVFNTLSPICVKNKENKCLNIDDSNFNKELNYITNKTLENFRGYGLLEELKFYPIQMKKKVVKEDISTFRENTKKQYYYVNSYAGIFKLEGNVNDLKDIYRLGVGFKRNQGFGMIEVLS
>NZ_CP013841.1|WP_101527649.1|924713_925154_+|WYL-domain-containing-protein
MYFVGYCELRQKIREFKISRIMSYEILGEKFERTYNFNFKEYMQDCFGIFKDEEIELKLKIKYPMSQIVKEKIWSDNQEIIDIEDKSIIFKAKLRGITEIKSWILSMGRSVEVIAPSSLRRNIENEIEKMIKIYKRVNSDLNMSGL
>NZ_CP013841.1|WP_101527648.1|924170_924689_+|HTH-domain-containing-protein
MSKVGNVFKMLILLKSRGKMKINDIARELEVSERQVRRYKNDLEQAGIYIKYTSGKYGGYEYDEKDYLLELNLTEEEDSVLFMALEQLKYDNFIGYKDFELIVDKINFLKETKTNKSVADVKYFVKNIKVNCNYEREKKNWIDINAAIITRSKIKIKYNSLNTGEKERIVRP
>NZ_CP013841.1|WP_012704810.1|935277_936075_+|4-hydroxy-tetrahydrodipicolinate-reductase
MKIIVIGPRGKMGSLVTKSCYNNENIELVAAVAPKGRDYIGQDLGIVANLGKNIDCKVVDNLDNVIDKCDCIIDFTDPETSMDVFEKALKSKKSVVCGTTGFSEEEINRINEISKEIPVVLAGNTSMVVNLMYKLVELAANTIGNMSDIEIIEMHDRYKKDAPSGTSLEIGEVLAKTFNKELKELAQFGRCGKGEREEGKIAYHSLRAGDISSSHTVMFGLMGERLEITHHAHNWECFANGGCQAALFLRDKKPGIYSMKDVLGL
>NZ_CP013841.1|WP_012704370.1|936096_936936_+|sulfurtransferase
MKNFVSTKWLQDHLEDENIVIVDCRGDLLKDSYGEEIYKKGHIKNAVFADLKEVMSSEEKEHGGRNPLPDVEDFKRSIEKLGINENTLVVAYDELRIAGAARFCWMLRYVGHTNNYVLDGGINKWITENRKLYIEENRSKEKLDIINGDFNISLNEEIKVDVNYIKDNMKKDLILVDSRTNIRYKGIEEPIDKRAGHIPGAKNVYWKDMLKEDGAVDENKVRENFLPLKDYNNIAAYCGSGIDATFNFLLLDEVGIKARVYAGSWSDWITYKDNPIDHK
>NZ_CP013841.1|WP_075143587.1|937448_939149_+|hypothetical-protein
MNTLEATKALDRIFSDWNIRFNNKSCVLIDGSWGIGKTKFIENYFSENEFIYTSVFGKNSVRDIEKAILIHSLPGLKKFNEDSGLAKVTQKIFNDISDKFLGVSIDSYLNSFSIDDIKLDISGNKRKIICFDDIERKSDSIEMKSLLGLIERAKKNFDVIIICNTHELDEKNNKTFNGYKEKVIDYVINISSVHEDTLNLILKNMDVENRNEIIDVYLKDNISFGKAPTGKKLFLKSKVHNLRVFIKYAELIMKLEKYLEPYKADEHILKICKAVIYDCYFSNKDVKKDSMSFDKFNIYKTINKILLNEDIKKDQFKEYFVANSEIRKDINNIYNAYKLTEKEFKNIIQRIESKIEDEDLEYFIKQENVISLVSVLNEFKILNKKMKVKLFKIAIDLYSPEKYITHTKIDYLKWNDFDYYGNEIECNKIVKSFIEKINEKCTEKFQNSIFNELEKSKNNKDYEKMLKLYKFNQIDKIEEFEDIFDYYFNQLVENYSCEISQKINTLISKTNSEIVGNFFTNRIKNETEVTKIKKYEQFDFELERKMQLEAEEEYYINNPPEEIEEC
>NZ_CP013841.1|WP_075143588.1|939591_940644_+|DUF4935-domain-containing-protein
MIYIFVDTNIYIKLLTNLGDESFFEELKVLIKERMIKLLVPEIVLLELDKQNRTVKHNFQNELNKLQVNIKEYSKSLWSEVRDIENKISSLIVKERKDKEEMWDRNYNTIVKYLTSSDVEYIEFTPRIMCNGEKRKISGKLVRTNETSSQDAYNIECLISYLNSIEENHSTEFIICTNDIKDFSKDKKSVKGFYKLHPIFECDFKNTKCVNSLENLMKYINYGFEYIEESSVDFTVIEEFNGTHDDLGGKEYSELYGKAERVCLENLNEIFMKKIIHTQNDLQKYRELVINDIYKLLNDCRESKEWSDRSELKLYSWLENREEDYICMSKLSDLILIRENMKRYLKIHLE
>NZ_CP013841.1|WP_101527653.1|940946_941993_+|hypothetical-protein
MVLVNIYGKIQPIIISKKLGVGICNLNSDWVRGFYDDFDEEILIHKHNMKKFLKDRFDIKMLEGRYNLSQITKVLGWQEEGKSVEQTIAEHLIEIQVIDFENSLLQKNNEHLDSPTCETQYTRKFIKFCSIVQSSESAQKVVSGQIPFVTYDSDNFSEKPRILNYLEVMSKTNTCINTYTNSCINTLQHLGGLIDGFLSADKDFWLLDYIIKAVYDERGTGAYHIFKVMSLIEMLIINPNENGRTVGEMERKLPQFLPITINTSQKELFSTIMRKLRNKIGHGDFMTIQQLLEEYRNSFMQNFWYDEFEYSIENWTYGNICLHLDKALANILWLMIFDKEKLSSIQQN
>NZ_CP013841.1|WP_075143590.1|944322_945993_+|hypothetical-protein
MVKKIKKKLKNNYLLWGIIVLIVPGIAIYLLNLLLKIGCYMGDWLSQYVDLSFIIIDTNSSISIGDYISYYFTIMALEVTGILSWALLKTSRKSNELSEAIKAKEDNRDNEKVKESAMIIYYDLLSNVRILKNLYVTLILNKETKSVKVIDINKDWIKNLSELRILLSKREIDVLFELYENFSSLSKAQVCSDAEAIKCITKGTANKLFINVLLKYLWMDFEGKIDSILHNKYYIIFRKIEAKINEEKECYSIQSNCLQMKQANGNEKIYGEYINGVLKNGTDSYYDENGHLIYSLTYKNYKVVKGKYFNYINDVFEKIFDVKFDENGIEDTGYIIIFYDSCKIKYKGGIKGEKYEGTGVLYQDSEYVSVIFDGSWEQGEKKEGTLKDNRKNSSIVYFKGSFNNDKPYTGEIKLGNLKTFKDVYEYIGTINVGKMENGFGYVKNTSFIDWDYIKKHSNDPSCQGYLMAKEEEEWNYRCGYEYEQEDIPNEVKQEMAEESARLEIQRSREDLQDTCLEVVELIGTRWTDGNPEKLEDDDLNKKYFGHGSKQKETSKK
>NZ_CP013841.1|WP_101527654.1|946306_946879_+|hypothetical-protein
MSFLAMDEEEYNFIWKTICELQLIFHPRYSREGEVDFKALSVLKDHKSIIVMLDRNLLSSLLRLSRDGYLKNEEEMRIIALLMTWLSMNNYPASAGLALQEFGTKINNEIGPKLELQEFNNIFKYYPGMIWLRLAEGAIDKIPVCSLPIEPFITEIKYSEEDDHLLMHIAEMLHVVYLYRNHNLSQLKKC
>NZ_CP013841.1|WP_158644205.1|946941_947358_+|hypothetical-protein
MLFTNQSGIKPPKNSGSNDIEKIFDGCKNQAWDLNYLSNWSSFHYNEKNMDDIFMFATNDILLKNIFINTYADGGVGALINTLFSKAYSQKIFEIINANQGSKRVKSNFGLNPKEYFRQLINQEKQKLQDTLGSYSNG
>NZ_CP013841.1|WP_045905527.1|947533_947863_+|hypothetical-protein
MDKFQSGLLAISSATLLIFIIYTLVNEKKSSKWFKWYNQMDSEEQKKYNPIIAVHKLKRVLFLIVLVGISGFLISFYIPLISMVAFVLTFIILLVSIGYLGPRGCKIEK
>NZ_CP013841.1|WP_075143591.1|947942_949391_+|serine-hydrolase
MKKTIVFVLTILILFSGFPTQSYPLSNAQSASIQALLDYACRISGVPGMSISILADDEVFYFSSGYADREKGLSASENTLYELASVSKAFTGIGILLMEKQGLLSMTDPIQKYLPWFTLKHQGKPIDMESITLNNFLHHTSGLTNGSHSQIIPQGNTPDMLQKTVEMLVDDELSFCPGEQYNYGTVNYDVLGLVIEIVSGERYEDYMKKQVFQPLGLHQTYVYKEDAQATGQLAQGYRSSFFMTTPYDAPDYAGNKPAGYIISCAKDMARWMGIQMGIVHDIPEIFHEIIEKSHHGDMSVPDVNGMFYAAGWSVNSEQTIIEHSGGNPNFATEVEILPKERTAVCLLTNGANTNRNLVLKIKEILDGNLSQSYEISGTQFLDITLSSATIICCLLAVLFFLLGLRRKKMNERQPMTKKRIIVTVILLIATIALCIMCCAFDWSTILIWQTYSVLTALISLALLTGSITWFVYTHRYNTSLRK

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_025146	Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111	192067-192103	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP013710	Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence	96511-96546	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NC_010418	Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence	7237-7272	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP013700	Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence	16949-16984	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NC_012654	Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence	227841-227876	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP014152	Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence	182839-182874	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP006909	Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence	9438-9473	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP013684	Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence	152047-152082	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NC_025146	Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111	130084-130119	0	1.0
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NZ_CP031095	Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence	154306-154341	0	1.0
NZ_CP013841_1	1.2\|933835\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	933835-933871	37	NZ_CP014218	Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence	4152-4188	1	0.973
NZ_CP013841_1	1.2\|933835\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	933835-933871	37	NZ_CP014173	Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence	46911-46947	1	0.973
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP014152	Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence	3429-3465	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP006909	Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence	71455-71491	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP013710	Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence	34844-34880	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_010418	Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence	211087-211123	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_012654	Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence	165930-165966	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP031095	Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence	216217-216253	2	0.946
NZ_CP013841_1	1.12\|934492\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934492-934528	37	NZ_CP013709	Clostridium botulinum strain F634 plasmid pRSJ2_2, complete sequence	115872-115908	2	0.946
NZ_CP013841_1	1.12\|934492\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934492-934528	37	NZ_CP013844	Clostridium botulinum strain A634 plasmid pRSJ19_2, complete sequence	161309-161345	2	0.946
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_025146	Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111	182486-182522	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP014152	Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence	235436-235472	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP006909	Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence	61844-61880	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP013710	Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence	43913-43949	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_010418	Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence	220234-220270	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NC_012654	Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence	175460-175496	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP031095	Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence	206687-206723	6	0.838
NZ_CP013841_1	1.5\|934032\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934032-934068	37	NZ_CP013700	Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence	232004-232040	6	0.838
NZ_CP013841_1	1.4\|933966\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	933966-934002	37	NC_010418	Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence	74306-74342	9	0.757
NZ_CP013841_1	1.6\|934098\|36\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	934098-934133	36	NC_013940	Deferribacter desulfuricans SSM1 megaplasmid pDF308, complete sequence	43415-43450	9	0.75
NZ_CP013841_1	1.2\|933835\|37\|NZ_CP013841\|PILER-CR,CRISPRCasFinder,CRT	933835-933871	37	MF595878	Caldibacillus phage CBP1, complete genome	31153-31189	13	0.649

1. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_025146 (Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111) position: , mismatch: 0, identity: 1.0

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataagctataaggtag	Protospacer
*************************************

2. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013710 (Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

3. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_010418 (Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

4. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013700 (Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

5. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_012654 (Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

6. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014152 (Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

7. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP006909 (Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

8. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013684 (Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

9. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_025146 (Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

10. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP031095 (Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence) position: , mismatch: 0, identity: 1.0

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
aaattatgtttataaatgataaaatagaaaataaac	Protospacer
************************************

11. spacer 1.2|933835|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014218 (Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence) position: , mismatch: 1, identity: 0.973

tagtttctttaaggtcttcaggaatatttttaatata	CRISPR spacer
tagtttctttaagatcttcaggaatatttttaatata	Protospacer
*************.***********************

12. spacer 1.2|933835|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014173 (Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence) position: , mismatch: 1, identity: 0.973

tagtttctttaaggtcttcaggaatatttttaatata	CRISPR spacer
tagtttctttaagatcttcaggaatatttttaatata	Protospacer
*************.***********************

13. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014152 (Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactataaagtag	Protospacer
*************************.******.****

14. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP006909 (Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactaaaaggtag	Protospacer
*************************.*** *******

15. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013710 (Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactataaagtag	Protospacer
*************************.******.****

16. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_010418 (Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactataaagtag	Protospacer
*************************.******.****

17. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_012654 (Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactaaaaggtag	Protospacer
*************************.*** *******

18. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP031095 (Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence) position: , mismatch: 2, identity: 0.946

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttatagtttaaagccataaactaaaaggtag	Protospacer
*************************.*** *******

19. spacer 1.12|934492|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013709 (Clostridium botulinum strain F634 plasmid pRSJ2_2, complete sequence) position: , mismatch: 2, identity: 0.946

tatcaaatcagaatgggaagtagcttagatgaaacat	CRISPR spacer
tatcaaattaggatgggaagtagcttagatgaaacat	Protospacer
********.**.*************************

20. spacer 1.12|934492|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013844 (Clostridium botulinum strain A634 plasmid pRSJ19_2, complete sequence) position: , mismatch: 2, identity: 0.946

tatcaaatcagaatgggaagtagcttagatgaaacat	CRISPR spacer
tatcaaattaggatgggaagtagcttagatgaaacat	Protospacer
********.**.*************************

21. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_025146 (Clostridium botulinum plasmid pCB111 DNA, complete sequence, strain: 111) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

22. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014152 (Clostridium botulinum strain BrDura plasmid pRSJ20_1, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

23. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP006909 (Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

24. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013710 (Clostridium botulinum strain F634 plasmid pRSJ2_3, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

25. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_010418 (Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

26. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_012654 (Clostridium botulinum Ba4 str. 657 plasmid pCLJ, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

27. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP031095 (Clostridium botulinum strain CFSAN034200 plasmid p1_CDC51232, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

28. spacer 1.5|934032|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013700 (Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence) position: , mismatch: 6, identity: 0.838

ttaattttatagtttaaagccataagctataaggtag	CRISPR spacer
ttaattttttagtttaaagccataaactataaaaaat	Protospacer
******** ****************.******.. *

29. spacer 1.4|933966|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_010418 (Clostridium botulinum A3 str. Loch Maree plasmid pCLK, complete sequence) position: , mismatch: 9, identity: 0.757

actacctattgctattttaacgaatgtatgatttcat	CRISPR spacer
tttcctagctactattttaatgaatgtatgatttcat	Protospacer
 .* *. ..*.*********.****************

30. spacer 1.6|934098|36|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to NC_013940 (Deferribacter desulfuricans SSM1 megaplasmid pDF308, complete sequence) position: , mismatch: 9, identity: 0.75

aaattatgtttataaatgataaaatagaaaataaac	CRISPR spacer
ctgtaattattatacatgataaaaaagaaaataaat	Protospacer
  .* **  ***** ********* **********.

31. spacer 1.2|933835|37|NZ_CP013841|PILER-CR,CRISPRCasFinder,CRT matches to MF595878 (Caldibacillus phage CBP1, complete genome) position: , mismatch: 13, identity: 0.649

tagtttctttaaggtcttcaggaatatttttaatata	CRISPR spacer
gcttttctttgagttcttcaggaatatttgcctctct	Protospacer
   *******.** *************** .  . .

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

1727252 : 1735451

uncultured_phage(33.33%)

protease

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_025775320.1\|1727252_1728197_-	YafY family transcriptional regulator	A0A1B0RXM1	Streptococcus_phage	3.2e-14	31.1
WP_101527759.1\|1728810_1729806_+	2-hydroxyacyl-CoA dehydratase	NA	NA	NA	NA
WP_045883004.1\|1729922_1730684_+	2-hydroxyglutaryl-CoA dehydratase	NA	NA	NA	NA
WP_045883006.1\|1730700_1731132_+	6-carboxytetrahydropterin synthase QueD	A0A1U9WRB3	Streptococcus_virus	2.3e-12	33.1
WP_025775316.1\|1731133_1731799_+	putative 7-carboxy-7-deazaguanine synthase QueE	S4TZT1	uncultured_phage	6.5e-38	44.6
WP_003358544.1\|1731802_1732393_+	GTP cyclohydrolase I FolE	S4U0J3	uncultured_phage	9.8e-46	54.5
WP_012704981.1\|1732576_1733236_+	7-cyano-7-deazaguanine synthase QueC	A0A2H4J8Q7	uncultured_Caudovirales_phage	2.3e-59	51.2
WP_003358688.1\|1733529_1734348_+\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
WP_012099663.1\|1734494_1735451_+	chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	7.7e-16	33.5

DBSCAN-SWA_2

2419339 : 2475893

Clostridium_phage(39.58%)

tail,holin,capsid,portal,integrase

attL 2432095:2432113|attR 2475804:2475822

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_075144042.1\|2419339_2419744_-	hypothetical protein	A0A2H4J7M8	uncultured_Caudovirales_phage	4.8e-12	37.3
WP_164071459.1\|2420099_2420267_-	hypothetical protein	A0A2H4J4S7	uncultured_Caudovirales_phage	2.1e-14	71.7
WP_101527857.1\|2420477_2420678_-	helix-turn-helix domain-containing protein	A0A2H4J765	uncultured_Caudovirales_phage	2.5e-17	70.8
WP_012047663.1\|2421705_2421927_-	hypothetical protein	NA	NA	NA	NA
WP_101527859.1\|2422555_2425198_-	hypothetical protein	A0A1V0E026	Clostridioides_phage	3.9e-86	31.1
WP_158644207.1\|2425215_2426610_-	hypothetical protein	A0A1V0E017	Clostridioides_phage	4.8e-43	30.4
WP_101527861.1\|2427217_2428042_-	glycosyl hydrolase	D9ZNF3	Clostridium_phage	1.4e-50	44.6
WP_025775134.1\|2428076_2428493_-\|holin	phage holin family protein	D2XPZ9	Bacillus_virus	6.3e-23	42.2
WP_061311424.1\|2428590_2428773_-	hypothetical protein	A0A2H4JGH3	uncultured_Caudovirales_phage	1.6e-07	65.2
WP_003356147.1\|2429244_2429649_-	hypothetical protein	NA	NA	NA	NA
WP_003356002.1\|2429897_2430059_-	hypothetical protein	J9QDY8	Clostridium_phage	2.3e-10	60.0
WP_101527862.1\|2430058_2430358_-	hypothetical protein	B6SBV1	Clostridium_virus	4.5e-15	51.0
WP_101527863.1\|2430368_2431673_-	methyl-accepting chemotaxis protein	E2ELJ8	Clostridium_phage	6.5e-66	43.3
WP_101527864.1\|2431672_2432767_-	hypothetical protein	E2ELJ7	Clostridium_phage	5.2e-93	47.9
2432095:2432113	attL	NA	NA	NA	NA
WP_053341146.1\|2432769_2433639_-\|tail	phage tail family protein	E2ELJ6	Clostridium_phage	1.0e-83	55.2
WP_101527865.1\|2433643_2439526_-\|tail	phage tail tape measure protein	A0A2H4JDX3	uncultured_Caudovirales_phage	1.7e-41	29.9
WP_061310218.1\|2439586_2440216_-	DUF4878 domain-containing protein	NA	NA	NA	NA
WP_169929191.1\|2440291_2440468_-	hypothetical protein	A0A0A8WHP4	Clostridium_phage	9.4e-05	51.2
WP_101527866.1\|2440457_2440877_-	hypothetical protein	NA	NA	NA	NA
WP_101527867.1\|2440932_2441559_-	hypothetical protein	A0A1U9WR69	Streptococcus_virus	7.2e-39	44.6
WP_101527868.1\|2441558_2441897_-	hypothetical protein	NA	NA	NA	NA
WP_101527869.1\|2441898_2442273_-	hypothetical protein	E7DNB1	Pneumococcus_phage	9.0e-13	31.8
WP_101527870.1\|2442265_2442631_-	hypothetical protein	NA	NA	NA	NA
WP_101527871.1\|2442627_2442936_-	hypothetical protein	A0A2H4J040	uncultured_Caudovirales_phage	4.6e-31	61.2
WP_075143170.1\|2442938_2443145_-	hypothetical protein	NA	NA	NA	NA
WP_024931284.1\|2443201_2444254_-\|capsid	major capsid protein	D9ZND6	Clostridium_phage	4.4e-89	51.4
WP_024931285.1\|2444267_2444660_-	hypothetical protein	NA	NA	NA	NA
WP_024933430.1\|2444680_2445295_-\|capsid	phage capsid scaffolding protein	A0A1Z1LZK8	Bacillus_phage	1.9e-07	26.8
WP_024933429.1\|2445426_2445954_-	hypothetical protein	NA	NA	NA	NA
WP_024933428.1\|2446090_2446387_-	hypothetical protein	NA	NA	NA	NA
WP_101527872.1\|2446396_2447362_-	hypothetical protein	A0A2H4J048	uncultured_Caudovirales_phage	1.1e-86	50.0
WP_101527873.1\|2447361_2448873_-\|portal	phage portal protein	D9ZNC8	Clostridium_phage	1.7e-139	52.0
WP_101527874.1\|2449070_2449805_-	HNH endonuclease	A8YQN4	Lactobacillus_phage	6.3e-18	48.7
WP_101527875.1\|2451232_2451940_-	hypothetical protein	A0A0A8WFS6	Clostridium_phage	2.0e-61	56.0
WP_012705283.1\|2452036_2452276_-	PepSY domain-containing protein	NA	NA	NA	NA
WP_101527876.1\|2452391_2452610_-	hypothetical protein	A0A0A7RTM8	Clostridium_phage	2.4e-10	55.6
WP_101527877.1\|2452701_2453805_-	Ig domain-containing protein	A0A0K2FHC7	Achromobacter_phage	4.0e-08	47.7
WP_045540836.1\|2454132_2454540_-	hypothetical protein	NA	NA	NA	NA
WP_014521162.1\|2454550_2454748_-	hypothetical protein	NA	NA	NA	NA
WP_061310205.1\|2454744_2454936_-	hypothetical protein	NA	NA	NA	NA
WP_101527879.1\|2455023_2455362_-	hypothetical protein	NA	NA	NA	NA
WP_101527880.1\|2455375_2456371_-\|integrase	tyrosine-type recombinase/integrase	S5M872	Bacillus_phage	3.0e-07	22.4
WP_167336337.1\|2456445_2456613_-	hypothetical protein	I1TJZ5	Clostridium_phage	6.6e-16	69.1
WP_101528076.1\|2456766_2457030_-	DUF1599 domain-containing protein	A0A2H4J4N1	uncultured_Caudovirales_phage	1.6e-32	80.5
WP_169929192.1\|2457299_2457437_-	hypothetical protein	A0A0A7RTX2	Clostridium_phage	4.3e-05	46.7
WP_169929193.1\|2457447_2457597_-	hypothetical protein	NA	NA	NA	NA
WP_101527881.1\|2458841_2459189_-	hypothetical protein	A0A0A7RTL9	Clostridium_phage	6.1e-40	73.7
WP_101527882.1\|2459191_2459596_-	DUF1064 domain-containing protein	D0R7I5	Paenibacillus_phage	4.5e-10	38.9
WP_169929194.1\|2459592_2459757_-	hypothetical protein	NA	NA	NA	NA
WP_101527883.1\|2460186_2460900_-	hypothetical protein	A0A0A7RWW3	Clostridium_phage	2.5e-72	62.3
WP_101527884.1\|2460926_2461319_-	replication terminator protein	A0A0A7RUF3	Clostridium_phage	4.2e-37	62.3
WP_075144091.1\|2461412_2461610_-	amino acid transporter	NA	NA	NA	NA
WP_101527885.1\|2461665_2461842_-	DUF3797 domain-containing protein	A0A0H3UYX0	Geobacillus_virus	4.7e-12	54.7
WP_158644208.1\|2461873_2462353_-	hypothetical protein	A0A1L2JY27	Aeribacillus_phage	2.5e-15	40.8
WP_101527887.1\|2462352_2462592_-	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	A0A2I7SC16	Paenibacillus_phage	1.0e-17	56.4
WP_101527888.1\|2462593_2462875_-	hypothetical protein	NA	NA	NA	NA
WP_169929195.1\|2462871_2463195_-	hypothetical protein	NA	NA	NA	NA
WP_169929196.1\|2463224_2464220_-	sigma-70 family RNA polymerase sigma factor	A0A0A0RV91	Bacillus_phage	2.0e-06	23.0
WP_101527890.1\|2464232_2464466_-	hypothetical protein	A0A0A7RTQ4	Clostridium_phage	4.9e-09	37.3
WP_163201000.1\|2464482_2464623_-	hypothetical protein	NA	NA	NA	NA
WP_101527891.1\|2464628_2465942_-	replicative DNA helicase	A0A1P8VVQ6	Streptococcus_phage	2.9e-74	37.9
WP_101527892.1\|2465938_2466685_-	helix-turn-helix domain-containing protein	A0A0A8WFH2	Clostridium_phage	1.4e-25	36.1
WP_047403403.1\|2466699_2466939_-	hypothetical protein	NA	NA	NA	NA
WP_158644209.1\|2467089_2467266_-	hypothetical protein	NA	NA	NA	NA
WP_169929197.1\|2467281_2467428_-	hypothetical protein	NA	NA	NA	NA
WP_101527893.1\|2467430_2468315_-	recombinase RecT	A0A139ZPK0	Marinitoga_camini_virus	2.9e-41	43.3
WP_101527894.1\|2468327_2469377_-	YqaJ viral recombinase family protein	A8ATY5	Listeria_phage	3.9e-21	26.7
WP_169929198.1\|2469624_2469792_-	hypothetical protein	NA	NA	NA	NA
WP_003392444.1\|2469800_2469959_-	hypothetical protein	NA	NA	NA	NA
WP_075144100.1\|2470011_2470164_-	Com family DNA-binding transcriptional regulator	NA	NA	NA	NA
WP_101527895.1\|2470184_2470394_-	hypothetical protein	NA	NA	NA	NA
WP_012704315.1\|2470405_2471194_-	phage antirepressor KilAC domain-containing protein	A0A0E3T9K5	Staphylococcus_phage	2.4e-87	64.1
WP_012703750.1\|2471247_2471469_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_101527896.1\|2471601_2472060_+	helix-turn-helix transcriptional regulator	S6C481	Thermus_phage	7.7e-06	31.9
WP_024933396.1\|2472106_2472541_+	ImmA/IrrE family metallo-endopeptidase	D6R412	Bacillus_phage	3.7e-26	47.9
WP_158000837.1\|2472736_2474176_+	DUF4041 domain-containing protein	X5JAC1	Clostridium_phage	3.3e-55	48.6
WP_101527898.1\|2474270_2475893_+	recombinase family protein	A0A1L2BY67	Clostridium_phage	4.1e-94	37.5
2475804:2475822	attR	NA	NA	NA	NA

DBSCAN-SWA_3

3061912 : 3071334

Synechococcus_phage(42.86%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_101528003.1\|3061912_3063412_-	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	3.2e-69	47.2
WP_012705191.1\|3063625_3064243_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	2.6e-25	40.6
WP_012099415.1\|3064370_3065366_-	phosphoribosylformylglycinamidine cyclo-ligase	M4QRQ6	Synechococcus_phage	9.9e-67	43.7
WP_169929208.1\|3065426_3066869_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.2e-57	34.9
WP_012047943.1\|3066966_3067671_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	6.0e-42	42.1
WP_045905308.1\|3067670_3068150_-	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	2.8e-27	49.4
WP_003357997.1\|3068778_3071334_-	selenium-dependent xanthine dehydrogenase	A0A0P0IVM8	Acinetobacter_phage	1.3e-09	32.4

DBSCAN-SWA_4

3218027 : 3240231

Clostridium_phage(80.95%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_101528024.1\|3218027_3219113_-	signal peptidase II	A0A0A7RU66	Clostridium_phage	4.6e-142	70.9
WP_101528025.1\|3219124_3220123_-	hypothetical protein	A0A0A7RW91	Clostridium_phage	2.1e-141	72.9
WP_025775560.1\|3220134_3220410_-	hypothetical protein	A0A223LE82	Bacillus_phage	5.1e-05	38.8
WP_101528026.1\|3220412_3222386_-	hypothetical protein	A0A0A7RU09	Clostridium_phage	4.1e-56	48.1
WP_075144313.1\|3222397_3223906_-	hypothetical protein	A0A0A7RU28	Clostridium_phage	5.5e-93	37.7
WP_075144314.1\|3223928_3224906_-	hypothetical protein	A0A0A7RU61	Clostridium_phage	2.7e-109	60.8
WP_075144315.1\|3224907_3226248_-	caspase family protein	A0A0A7RW86	Clostridium_phage	2.1e-75	74.6
WP_045905342.1\|3226262_3226604_-	hypothetical protein	A0A0A7S0S4	Clostridium_phage	1.6e-16	55.7
WP_071647550.1\|3226616_3227702_-	hypothetical protein	NA	NA	NA	NA
WP_025774830.1\|3227688_3228123_-	hypothetical protein	NA	NA	NA	NA
WP_025774832.1\|3228135_3229539_-	hypothetical protein	A0A0A7RU22	Clostridium_phage	6.9e-05	42.4
WP_003483832.1\|3229702_3229945_-	hypothetical protein	A0A0A7RW80	Clostridium_phage	4.4e-21	67.6
WP_045881634.1\|3229904_3230321_-	hypothetical protein	A0A0A7S0S0	Clostridium_phage	9.0e-46	71.2
WP_012100776.1\|3230335_3231223_-	hypothetical protein	A0A0A7RTZ9	Clostridium_phage	2.6e-119	74.7
WP_061310725.1\|3231227_3231647_-	hypothetical protein	A0A0A7RU17	Clostridium_phage	1.8e-57	72.7
WP_045881637.1\|3231652_3232000_-	hypothetical protein	A0A0A7RU51	Clostridium_phage	1.9e-33	60.7
WP_012048059.1\|3232004_3232370_-	hypothetical protein	A0A0A7RW73	Clostridium_phage	5.0e-32	56.2
WP_012048060.1\|3232369_3232654_-	hypothetical protein	A0A0A7S0R4	Clostridium_phage	1.0e-24	60.2
WP_045881639.1\|3233339_3233861_-	hypothetical protein	A0A0A7RVS1	Clostridium_phage	1.1e-35	50.0
WP_012704130.1\|3233973_3234294_-	hypothetical protein	NA	NA	NA	NA
WP_052704682.1\|3234350_3235151_-	ATP-binding protein	A0A2K9V3L7	Faecalibacterium_phage	3.7e-32	34.8
WP_012705580.1\|3235140_3236046_-	phage replisome organizer N-terminal domain-containing protein	A8ASN4	Listeria_phage	9.4e-40	39.5
WP_012704458.1\|3236139_3236352_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_012704308.1\|3236413_3236623_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_003360073.1\|3236814_3237225_+	helix-turn-helix domain-containing protein	A0A0A7RUJ5	Clostridium_phage	1.0e-09	56.2
WP_012705276.1\|3237526_3237676_+	hypothetical protein	NA	NA	NA	NA
WP_003357882.1\|3238743_3240231_+	MBOAT family protein	A0A125RNP0	Pseudomonas_phage	4.3e-66	38.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage