CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP024870	Spiroplasma clarkii strain CN-5 chromosome, complete genome	2 crisprs	cas2,cas1,cas9	0	8	1	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP024870_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP024870_1

957797-958824

TypeII

Consensus_repeat	Method
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	PILER-CR
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	CRISPRCasFinder
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	CRT

15 spacers

cas1,cas2,cas9

The CRISPR arrays of NZ_CP024870_1

>merge|NZ_CP024870|1|957797-958824|PILER-CR,CRISPRCasFinder,CRT
GTCGTCTATTTGTTAGATATGTCAGATAACTAAAACAAATAGTTAGAAAGGAGAAACTACAATGGAGCGCCTATTCCTTAGATATGTCAGATAACTAAAACTGAGCGACTAAGCTGGAAGTTGAGGATGGATGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTTGTAGTAAACTCCTCATATTCGACCATGAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTCTTTTCTCGGTTATATGGCCCTAAGGCCAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACGGACTTGACCCATCACTTCGCTTAAACTCAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTGCACTTGTGTTTAATGCAAGTTTTAATGAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTTTGCTGAAATTTGGATTTTTGATTTTAAAGGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACGCAATTATGAACAAGGTGAGGGAGTGTATGGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACGATGGTAAGGAAGATTTTTATAGATCATCAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTAGACTTAACAGGTACCTTTGGTAGTTTAAAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACCTTTCTATAATAGTCTAGTACAAACTCAGCGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACATGAATGAAGTATGATTAACTACTTAAGTGGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTTCTTTTCTTTTCTCGGAAATGGTGCCTTAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACTCCTGGCAAATTGAGCAGACAAAGCATTTAGTCGCCTATTCCTTAGATATGTCAGATAACTAAAACGCACTTGAGCACAATTGAATTTGATAAAACGTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC

>NZ_CP024870|1|1|957797-958824|PILER-CR
GTCGTCTATTTGTTAGATATGTCAGATAACTAAAAC	AAATAGTTAGAAAGGAGAAACTACAATGG
AGCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGAGCGACTAAGCTGGAAGTTGAGGATGGAT
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTGTAGTAAACTCCTCATATTCGACCATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCTTTTCTCGGTTATATGGCCCTAAGGCCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GGACTTGACCCATCACTTCGCTTAAACTCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGCACTTGTGTTTAATGCAAGTTTTAATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTTGCTGAAATTTGGATTTTTGATTTTAAAG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCAATTATGAACAAGGTGAGGGAGTGTATG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GATGGTAAGGAAGATTTTTATAGATCATCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TAGACTTAACAGGTACCTTTGGTAGTTTAAA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	CTTTCTATAATAGTCTAGTACAAACTCAGC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	ATGAATGAAGTATGATTAACTACTTAAGTG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTCTTTTCTTTTCTCGGAAATGGTGCCTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCCTGGCAAATTGAGCAGACAAAGCATTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCACTTGAGCACAATTGAATTTGATAAAAC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC

>NZ_CP024870|1|1|957797-958824|CRISPRCasFinder
GTCGTCTATTTGTTAGATATGTCAGATAACTAAAAC	AAATAGTTAGAAAGGAGAAACTACAATGG
AGCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGAGCGACTAAGCTGGAAGTTGAGGATGGAT
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTGTAGTAAACTCCTCATATTCGACCATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCTTTTCTCGGTTATATGGCCCTAAGGCCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GGACTTGACCCATCACTTCGCTTAAACTCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGCACTTGTGTTTAATGCAAGTTTTAATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTTGCTGAAATTTGGATTTTTGATTTTAAAG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCAATTATGAACAAGGTGAGGGAGTGTATG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GATGGTAAGGAAGATTTTTATAGATCATCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TAGACTTAACAGGTACCTTTGGTAGTTTAAA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	CTTTCTATAATAGTCTAGTACAAACTCAGC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	ATGAATGAAGTATGATTAACTACTTAAGTG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTCTTTTCTTTTCTCGGAAATGGTGCCTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCCTGGCAAATTGAGCAGACAAAGCATTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCACTTGAGCACAATTGAATTTGATAAAAC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC

>NZ_CP024870|1|1|957797-958824|CRT
GTCGTCTATTTGTTAGATATGTCAGATAACTAAAAC	AAATAGTTAGAAAGGAGAAACTACAATGG
AGCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGAGCGACTAAGCTGGAAGTTGAGGATGGAT
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTGTAGTAAACTCCTCATATTCGACCATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCTTTTCTCGGTTATATGGCCCTAAGGCCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GGACTTGACCCATCACTTCGCTTAAACTCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TGCACTTGTGTTTAATGCAAGTTTTAATGA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTTGCTGAAATTTGGATTTTTGATTTTAAAG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCAATTATGAACAAGGTGAGGGAGTGTATG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GATGGTAAGGAAGATTTTTATAGATCATCA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TAGACTTAACAGGTACCTTTGGTAGTTTAAA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	CTTTCTATAATAGTCTAGTACAAACTCAGC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	ATGAATGAAGTATGATTAACTACTTAAGTG
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TTCTTTTCTTTTCTCGGAAATGGTGCCTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	TCCTGGCAAATTGAGCAGACAAAGCATTTA
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC	GCACTTGAGCACAATTGAATTTGATAAAAC
GTCGCCTATTCCTTAGATATGTCAGATAACTAAAAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP024870.1\|WP_100254698.1\|949155_949851_-\|MurR/RpiR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224651
NZ_CP024870.1\|WP_100254709.1\|965303_965924_-\|bifunctional-4-hydroxy-2-oxoglutarate-aldolase/2-dehydro-3-deoxy-phosphogluconate-aldolase	unknown	unknown	gnl\|CDD\|188632
NZ_CP024870.1\|WP_146637953.1\|950492_951161_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254695.1\|947429_947708_-\|PTS-sugar-transporter-subunit-IIB	unknown	unknown	gnl\|CDD\|99905
NZ_CP024870.1\|WP_100254700.1\|951401_952472_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254702.1\|955198_956386_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_157795148.1\|958833_962358_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|187774
NZ_CP024870.1\|WP_169921852.1\|948162_949005_-\|class-II-fructose-bisphosphate-aldolase	unknown	unknown	gnl\|CDD\|376459
NZ_CP024870.1\|WP_100254710.1\|965952_967986_-\|heparinase-II/III-family-protein	unknown	unknown	gnl\|CDD\|379893
NZ_CP024870.1\|WP_100254701.1\|952719_954696_-\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|226121
NZ_CP024870.1\|WP_100254703.1\|956388_956697_-\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
NZ_CP024870.1\|WP_100254707.1\|963498_964275_-\|2-dehydro-3-deoxy-D-gluconate-5-dehydrogenase-KduD	unknown	unknown	gnl\|CDD\|180761
NZ_CP024870.1\|WP_100254706.1\|962648_963488_-\|5-dehydro-4-deoxy-D-glucuronate-isomerase	unknown	unknown	gnl\|CDD\|179169
NZ_CP024870.1\|WP_100254708.1\|964284_965304_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|238571
NZ_CP024870.1\|WP_100254714.1\|971001_971229_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254704.1\|956707_957646_-\|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|274694
NZ_CP024870.1\|WP_100254713.1\|969958_971002_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254712.1\|969164_969953_-\|MurR/RpiR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224651
NZ_CP024870.1\|WP_100254711.1\|968030_969161_-\|glycoside-hydrolase-family-88-protein	unknown	unknown	gnl\|CDD\|369381
NZ_CP024870.1\|WP_100254696.1\|947720_948161_-\|PTS-sugar-transporter-subunit-IIA	unknown	unknown	gnl\|CDD\|238129

Protein	Function_ID	Function_description	E-value
NZ_CP024870.1\|WP_100254698.1\|949155_949851_-\|MurR/RpiR-family-transcriptional-regulator	gnl\|CDD\|224651	COG1737, RpiR, Transcriptional regulators [Transcription].	1.26111e-29
NZ_CP024870.1\|WP_100254708.1\|964284_965304_-\|hypothetical-protein	gnl\|CDD\|238571	cd01166, KdgK, 2-keto-3-deoxygluconate kinase (KdgK) phosphorylates 2-keto-3-deoxygluconate (KDG) to form 2-keto-3-deoxy-6-phosphogluconate (KDGP). KDG is the common intermediate product, that allows organisms to channel D-glucuronate and/or D-galacturinate into the glycolysis and therefore use polymers, like pectin and xylan as carbon sources.	3.65315e-49
NZ_CP024870.1\|WP_100254695.1\|947429_947708_-\|PTS-sugar-transporter-subunit-IIB	gnl\|CDD\|99905	cd05563, PTS_IIB_ascorbate, PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.	4.1205e-19
NZ_CP024870.1\|WP_100254709.1\|965303_965924_-\|bifunctional-4-hydroxy-2-oxoglutarate-aldolase/2-dehydro-3-deoxy-phosphogluconate-aldolase	gnl\|CDD\|188632	cd00452, KDPG_aldolase, KDPG and KHG aldolase. KDPG and KHG aldolase. This family belongs to the class I adolases whose reaction mechanism involves Schiff base formation between a substrate carbonyl and lysine residue in the active site. 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, is best known for its role in the Entner-Doudoroff pathway of bacteria, where it catalyzes the reversible cleavage of KDPG to pyruvate and glyceraldehyde-3-phosphate. 2-keto-4-hydroxyglutarate (KHG) aldolase, which has enzymatic specificity toward glyoxylate, forming KHG in the presence of pyruvate, and is capable of regulating glyoxylate levels in the glyoxylate bypass, an alternate pathway when bacteria are grown on acetate carbon sources.	3.44453e-63
NZ_CP024870.1\|WP_100254711.1\|968030_969161_-\|glycoside-hydrolase-family-88-protein	gnl\|CDD\|369381	pfam07470, Glyco_hydro_88, Glycosyl Hydrolase Family 88. Unsaturated glucuronyl hydrolase catalyzes the hydrolytic release of unsaturated glucuronic acids from oligosaccharides (EC:3.2.1.-) produced by the reactions of polysaccharide lyases.	2.99798e-17
NZ_CP024870.1\|WP_157795148.1\|958833_962358_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl\|CDD\|187774	cd09643, Csn1, CRISPR/Cas system-associated protein Cas9. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II.	1.91851e-96
NZ_CP024870.1\|WP_100254706.1\|962648_963488_-\|5-dehydro-4-deoxy-D-glucuronate-isomerase	gnl\|CDD\|179169	PRK00924, PRK00924, 5-keto-4-deoxyuronate isomerase; Provisional.	2.24206e-135
NZ_CP024870.1\|WP_100254701.1\|952719_954696_-\|AAA-family-ATPase	gnl\|CDD\|226121	COG3593, COG3593, Predicted ATP-dependent endonuclease of the OLD family [DNA replication, recombination, and repair].	2.41071e-07
NZ_CP024870.1\|WP_100254703.1\|956388_956697_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	8.85751e-25
NZ_CP024870.1\|WP_169921852.1\|948162_949005_-\|class-II-fructose-bisphosphate-aldolase	gnl\|CDD\|376459	pfam01116, F_bP_aldolase, Fructose-bisphosphate aldolase class-II.	3.07935e-113
NZ_CP024870.1\|WP_100254710.1\|965952_967986_-\|heparinase-II/III-family-protein	gnl\|CDD\|379893	pfam16889, Hepar_II_III_N, Heparinase II/III N-terminus. This is the N-terminal domain of heparinase II/III proteins. It is a toroid-like domain.	1.79593e-24
NZ_CP024870.1\|WP_100254704.1\|956707_957646_-\|type-II-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274694	TIGR03639, cas1_NMENI, CRISPR-associated endonuclease Cas1, subtype II/NMENI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is a prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 variant of the NMENI subtype of CRISPR/Cas system.	5.84075e-81
NZ_CP024870.1\|WP_100254696.1\|947720_948161_-\|PTS-sugar-transporter-subunit-IIA	gnl\|CDD\|238129	cd00211, PTS_IIA_fru, PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.	5.9863e-16
NZ_CP024870.1\|WP_100254712.1\|969164_969953_-\|MurR/RpiR-family-transcriptional-regulator	gnl\|CDD\|224651	COG1737, RpiR, Transcriptional regulators [Transcription].	1.23401e-28
NZ_CP024870.1\|WP_100254707.1\|963498_964275_-\|2-dehydro-3-deoxy-D-gluconate-5-dehydrogenase-KduD	gnl\|CDD\|180761	PRK06935, PRK06935, 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD.	6.21426e-137

>NZ_CP024870.1|WP_100254704.1|956707_957646_-|type-II-CRISPR-associated-endonuclease-Cas1
MSWKTLLITNYDKLSLKNGLLMVTNLKAKKELYFAMNDLNCVVLENNYTTLTTQVLSKLVENNVYLIICDDKYEPNGLIMSFHNHWKPLTIFENQIKLQNYQKEQLWKLVVEKKIENSINVMGMLGCSQRSLTMMSNFFNTVKLNDASNREALAAKVFFRELYGSSFLRFSEDIINAALNYGYTILASAISRTLVKYGLHCFLGIFHKGKTNNWNLSYDLVEPFRPMVDLWVSTNIESLLANETLHYQHRLELINLLNQSVVIDEKIHTVTNAIEIVVKSYYTYLKTASLTKLKLPTIVQFEERSSLDETEE
>NZ_CP024870.1|WP_100254703.1|956388_956697_-|CRISPR-associated-endonuclease-Cas2
MRLMVFYDLPNTTKSENKEYTKFRKFLIQNGFYMMQFSIYVKLCMNHDIVLITQKKIEKNKPKIGDVRMLAITEKQYNDIKILVGQKSIQESYQNIDYLVEL
>NZ_CP024870.1|WP_100254702.1|955198_956386_-|hypothetical-protein
MRNYLSLNSKYFGKVKISFFNKLAVLYGPNDSKKSLLLNELSKVLNTKSGKTSGLSFDLFENGASDFQVIYLKEVFELSSELKFSKTGFLKDSILKYLNKKIYEEKVINNFDQQLSDISKNLASAVEEYFAENHLLDEIVGKIDFKTELQLNSVEVILEKLVKLNIYNEDNRTVEEKNYSKFLVRMLLLKIILNDVDLSDTLRPVIILLDLPESCADDLSISKLRNWIKLFAQNQIAWIVTINNSHSLRLLNPNIQSVNFFEKDKINFVVNYKNYVKNITIFSAFLESKYSNLDQFKFDLNKVLTVDDLKIEGQKFQKYYENILYKCFIYKNIVINEDEALNFFWEVNTTNDLTFHLNAKNICIILMMIIELKLNLKVTFNVKTQKLKNLIDLVF
>NZ_CP024870.1|WP_100254701.1|952719_954696_-|AAA-family-ATPase
MSERYLKYKQYRNIAIDVSDTKDEKKVKLILNTSIRNLGELVVLIGPNNVGKSNILRSLNSLNQATLSTKLDKPSFYEYDKAEPEICLVYNENKYKCSYFINEKTNYYEHNLNGENIETKITVPQLVEKIIEFGILILNHYLEELSHENRNHDYQYGRNISQLIINAQNAISFFQNISDFNKYILHIDENDNVMFDLKGEMSTAVPTTYEAYLYLVKSGKYQWIKEKVDSSDEHIIGDEYISIRKMFANLISADVHISNIFTEKYGFNIVPKVYEFIEPNFNINNLTSRIDNDILPTNCFNIFFKNIILDKFKIDKDKINSIYEKEKSGSYPGSLRKLEAEINEKLIEVSKDFNAIYNKNKSKYKFSIEVSESKFSFAITSDNEVISLARQSEGFKWFFSFYFDFLITNNIKKGDIILIDEAGSKLHPQGCVELRKFLKKFALDNEVTIVIATHSPFMISNDHLDELRIIKQNKNSLDIINRFTVDEFDSSDSNVTKNILDALTVQKHVIFNPENTIIFVEGITDYCYLTAFKKLLNQDNVYFLPFQGVAGPEKILSELSNIMKNPVCLVDGDEAGSEFASKAKEKDIKCYSLKEAGSTFKDIEDLFSKEMREKFKLDSKKFNNAVQFKNAVENENIKVDEKTRKNFEKVFSLITRES
>NZ_CP024870.1|WP_100254700.1|951401_952472_-|hypothetical-protein
MILFEREMILDNDKKFSYLKNLLDNDFKLLDKNQIIFGCNGAGKSSISKFIKQNNNAVFYFDQTDEIEDFKNTDDWKKIRKYRRNKELIIKYNTLKIDEKEEEIELLKRENQINTNLIRVVGHSSKENTDVISERISSYWKDELFFDYEIPKEDLEYINSESNLEVIRQLLNYYVWKLFKEIELKDSESKKIRNSIIGYSFEILLKSELDDKTCPICSTQLESLREMLATKTKEFNVGKSKIFELFKNKSVIFSEESFKKFYELYLYLKNREDLLIGLAISECNLEKLKFICDNSKKINKAKRKLDKLIDESKSKFEILSKNKTEFIHNFSSYLKIDKRNINFNSFSYEIEIVLNR
>NZ_CP024870.1|WP_146637953.1|950492_951161_-|hypothetical-protein
MFVYKYLEKFKGVTSIKDIDTKDLNEHILSLDSLKEKCVKGDYYNALSALIKKENPSFNELDNIHKIFHYTIDEKINELNNSKYYINSEKLIDLIENYIELNNEDFFSNTIKKVVLLSSLRAWIESKIYSLISNEEVKSEFINCYTFNEKINIIFEKNGNLKVKLSKKLSRKYLMSKKVFLNHSVHYNSTVIPLQYPLSVSIDDINNDIQDLKEYFKNLQSL
>NZ_CP024870.1|WP_100254698.1|949155_949851_-|MurR/RpiR-family-transcriptional-regulator
MSTLTKREQEAYDYLKKHSDLLNTSVDNICKELKISYGTIYSLIEKLGYKGYKDLIINISSEIIKEKSGLSHNYHELIDANFKIDNEQNLEKFFKIIKDKTVVFIGKSHSHIIAKKFSLDFNRLGIKSYVLENDYDQIILSISNMTADCVLVAITNSGTTDWILSALKMANSQGIKCVTITCVNSKKLTELNELVITMVNRNKLMIKSRITNFIPILIFLDIFWEKAIDKL
>NZ_CP024870.1|WP_169921852.1|948162_949005_-|class-II-fructose-bisphosphate-aldolase
MLTNLKEILVAAYKGGYAIPAFNVDNYEIMKAIVDACEEQKHPVVLMFTQSAIKYYGLNNLIAVANNLANSVSIPVVTHLDHELDVETCKAAINEGIQSIMYDGSHCSLETNINNTNLVYDYAKSTLKEISLEAELGQIAGVEDWKKISKSVYTNPEDALRFWTATNIDCLAISIGTAHGMYQTKPSLNLELLETINKLLQKPLVLHGGSGLAKEDIQKCIKLGIAKINFGTEIKIKFASACVEYIKTNQDCQDVRKILTYAMTELKQLIYEKINICKGK
>NZ_CP024870.1|WP_100254696.1|947720_948161_-|PTS-sugar-transporter-subunit-IIA
MKYDKNLLNEYEGFTCWKEMLKTCGASLIVNNYITASYLEVIIASVIKHGPYFVVAEQVAIAHSDINLDNIKKTGVSWNILKKPCQIGDINPKAVKYLIILATTNGVEHLGFLKKFSQVFLDEAKRTEFYQIQTKEQLIIFLEKHF
>NZ_CP024870.1|WP_100254695.1|947429_947708_-|PTS-sugar-transporter-subunit-IIB
MYKFMSVCGSGLASSFLIEETIQAALKQLNTEGEVSHTSVGSFAPDWDLDFVIVGNDITDSVEFPRKITLDNLFDVEECKQKISQALTNYKK
>NZ_CP024870.1|WP_157795148.1|958833_962358_-|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MKKHILGLDLGISSVGWAVTTFDDEGGYEPWIDDFGVRLFEVPENPKSKVSLATERRMLRSSRRLNRRKKARIKLIKKTFEEYGIISVEEINNGVFDFKNSKLKPTNKVDYDENLFFNPYVLRNRALTQPLSKEQLVIVVTHIAKNRGFANLFDVNKGEDVSTLTDQSKVKEDDSYTNSIFEARKMIGYDLKTKKCQKTIAEAIIANEFGNTRVKNNDNLLDVRNNVKDKKSGEGTKSGDFKFLFPREAYQYELEMILNQQAKYFPEITSEFIKKIIETAFRQRDFEDGFGPKGYTRDQIKKKIQDQLAIMKAKNVKDRNFLALRDSLKAYKLYKPFTELTGKCTFYPDESRYVKSSIIFEIYQMAVEVSKFSSLLETEEKICEFHNKLFEKALKDDKFITGSSKAIDKILIEDFKVEKEEVKNSKAIENAKKTTLFEFTKRFIKVFGMEILKKIDSTTLDKNFIDDLGILLNQNITPKRREEKIRDWAKNNNLEIDNEQVNELLFLPAKVTTTSGLSKKAMLEVIEKFLEGKIAGVYQDEMKANAVEQTLAKPGRFLEPIKDPDLVRNPVVFRAINEARKVIKALFNRYGDFHKINVETSRELGKSAEVRKELNTRNLENRLNNMGISKILEDNGLRANHTNILKYKLWKSQDEKCMYSLETILLEQFDTYELEIDHILPISKFPDDSIENKVLVFNSENQKKKNRTPLEYLNADNPGVVSNFKKNCLNLYRKNKINWNKYENLMVESVMKIETDFAKRNLVDNSYIARYFANWLKQQLTFKYANEGKEYKSNVLMIKGIVTSRFRRKWLFNSPWGLDTKVRDITPYHHAVDAIVLAQFKNQGSIDFASDLLILGDEFRSWNKKIITDEQWNERKTAVFSKWRKENGYIWYHEIPNALERLENYINSGCKQTGMYALVKNLKEVIELRMPVELTIESIEETEKLDEKQIKRKIKETKRYEIFNGLTEFKRKVKTKVPKFVKVKDPKEYLKTLDDANIQGFKHYPFVSYKINKKLKGAVTDSQLIKGKNVAIDKKTGKFNSEKYFTSKNGDIWESTIYYGLKLNKLEQKAKPQWVKKVDIFKDKSILSLKGGEVLLVKNTTFSYINKHSEIEYKVLRGKKGDTSYVHKINTTFISDENKNKEIFKEQNFRATTSQLSNQISILNISILGYSLID
>NZ_CP024870.1|WP_100254706.1|962648_963488_-|5-dehydro-4-deoxy-D-glucuronate-isomerase
MKKIQLSDAVSKKEFSKLTKNDLVANFKFNLFETDEVKAVYTYYDRVLLMGAVPKTKLLEISKFGTELNAKYLLENREIGIINLAAPGIVIVENKEFLLNKNEAIYIGKGNEKIQLKSQNPDTPAMFYMVSTPAHKVLPTKIISINDAKVVNWGKKETLNERTIYQLIHPEVVETCQLSLGVTCLKPGSVWNTYPPHTHERRMECYLYFDLDHESEVYHFMGQKEDVRNIIMRDKDCVVNPPWSVHYGVGTKSYSFVWAMAGENKDWTDMDFIETKDLI
>NZ_CP024870.1|WP_100254707.1|963498_964275_-|2-dehydro-3-deoxy-D-gluconate-5-dehydrogenase-KduD
MKTNYVIETFGLQDKVVIITGGNTGLGQAYSDAMAQSGAKLFITCYDKTGVEEMQNLYDQNGWEVAFAFGDLTNPQIQDEIIAKCLKKYKRIDILVNNSGTISRNPILEGTDAEWNKVIDINMNVVYRLSREVSKIFKKQGGGKIINIASMLSFQGGKFVPSYTASKHGVAGLTKAFANELAEFNINVNAIAPGYIETKNTAPIRADVKRNSEILTRIPANRWGKPSDVAATCVFLASKASDYCNGFVFAVDGGWLAR
>NZ_CP024870.1|WP_100254708.1|964284_965304_-|hypothetical-protein
MSICFLGEPLLRFSTTNCKLLKDSINFNLSVGGTELNVAANISNLAAKPCADFYTVLPKNLLGEIVKRHCFKNRVNLIEGKQTSERMGLYFLEPKQGNKMGNVVFDRKNSAFAQSQATDYEIESKKYKIFHTSGIAIGTNPPTILNLVSYFFKNVNSDLYSFDCNYRSSMWSAQEAFASYQKLLTTAKINILFANSWDICQILKLTNLNIDINDKNQFAELCKLIFKKYKSLKYIICTKRNFKSNTETFGYQAMYFDGKDVYESSERYVATIIDRIGCGDAFVAGVLFKLLENSSIKEAVEYGLCCSWLKHSIYGDTLNLLQAEIEPYLEFDNSKLVNR
>NZ_CP024870.1|WP_100254709.1|965303_965924_-|bifunctional-4-hydroxy-2-oxoglutarate-aldolase/2-dehydro-3-deoxy-phosphogluconate-aldolase
MKNILESINQNKIIAVVRHKNYEEAKKMCLGCIEGGIKIIEITLTVNNAFELISEMVNLFPEIIFGAGTILTLADAKQEIKNGAEFLISSNTNLEILNWTAENQHLYIPGVMTINEIEFARSKGSQIQKLFPGDVLKPGFVKSVLAPMPDVKLIPSGGVDIKNAKEWLNNGVFAISVGGNLVGKINETINQDEVKQRSIKFLEAIN
>NZ_CP024870.1|WP_100254710.1|965952_967986_-|heparinase-II/III-family-protein
MKNNINLYEQFAVENLTELHNWCKNFDNKNNELKYLEEIVDYNLKNNQQTVSNTILEAELVVDNKFHFIDPWDMERCEQLISFTEINWEHKYNGDPEWMYMICRMSFLKKLARAYAYTKNPKFANKIIALVKYFSTKYSDYNGNENTTWRTIDAGTRFSNLIYAFLIIKNCEIISENDIEMFFKVFLKHIEFLTVKYESWKRLSNWGILQSLPLLQFSLIFKEIFLESNNLFELAMERLTQSLKIQILADGGHWEKSPLYHNEVLDTFSKVVNLCLINSIEIEDWILKKIILAHEYNLKILKPDRTQPMQGDSDNTNLTSRLQVAALLFQRQDFKFFTGETVYEENVWDYGFMLTKNYQNLKSKVPAFKSFCFPVLGEGYYRSSWEQDADWLWFSCGSLGSGHGHGNSLHFSLTINGKDFLIDPGRYTYKGTDKNREYLKSQAAHNTIIVDDTPTQKFKGSWEITKTPRYICANMQEYNNEYTFFEGTDLGRVDNGQVINRQILIVDNFATIIVDDLFNYANITNVKNNFILDKNVNVKEFENYFEMTNNGTSIYFYNLNQVKPKILRAVVSYNYNSIEDSKKVVCEISNPKTGMINIFAKSKLNIKKHDVFNKENIKLNDKKFKSFTLTNNNQSYTIYFAFEELVGGSPLVYINHETFYGKVVAKNNNSGQVTIIK
>NZ_CP024870.1|WP_100254711.1|968030_969161_-|glycoside-hydrolase-family-88-protein
MKNIDKAVNHIVEKMEQNYLTLNNLFPDSHTICGKYPKVSNYLIEAGWTSGFWTGMLWMMTMYSKEKKYQLLAEYHLTSFRARLREEILINHHDLGFLFLPSAYSQYKLTNFSKAKDTVVWAADKLCERFVVPGEYIQAWGEIDKNKNGKFIIDCNLNVPLLFKAHDLTGEQKYLDIAVKHLKTASQLLIRNDGSTFHSFNMNAIDGKPLFGETVQGFSDTSCWSRGQAWAVYGFAIAYKRTGLEIFKQPYFKVTSYFIKNLPNDLVPYWDLVFTDADSATQEKDTSAAAIFINGVLTMSETLGEENSEFKDIAIKCLESLTQNYLSDQNSEGILSQGVYNKKLNVGVNESVIWGDFYFMQAIFLLKGLDFYKIWE
>NZ_CP024870.1|WP_100254712.1|969164_969953_-|MurR/RpiR-family-transcriptional-regulator
MEFSILSKIKSACKLDLAENGIYVSIANILLEKLNVLDKISIISLSKEANVSPASITRFARKFGYSGWKDFIEALKREYNMSSTPIVSNSSKSKLLLYAKTITNSITAVAKENANSIDEVVAKLDNFKDCIYLFGIGANMDVIKIFSSELSALGFNVRYDSDYANQIRLSKIAKHEDLVFIFSHSMIGETIEAIFSNIKNQQCEIVLISHNKISDTIFINVPQNEAVHKNSHSKSSFAMLFLAYAIINNIIEIKLKNEKRTN
>NZ_CP024870.1|WP_100254713.1|969958_971002_-|hypothetical-protein
MILKITGYTLFATICLVVIAWLLFDQKKKRTKRAGMMPFLEQQRDRAAELFAELDVKKLSEVLKYCDGKAYQSSWFNNYDWKLFSNLKSQYDSFVLLVLNVEDNSVVPVYYSKCEYKNIYLFMKEIIYSIDIRIKDVDNKKQYEDVYLAIAEYLIFHQVSLDDIIFLPLKNEIDDSELLKISYTFGFNNVEKPRNPFELASKRLSINNSSNDISWNQDQLGERKYDDVLTQTKIKELKNEDDNKNMLLIWLENSSLDYQKNFKIPFLILNKKEIQKYFDEDENLETQIETIFNNSTFEEEVANPIYNLKQFKNKFGGELKWEIVDSKQSQIKKIIKSKSNLIRTLRV
>NZ_CP024870.1|WP_100254714.1|971001_971229_-|hypothetical-protein
MEILGWVLIGLGSLALIIVTIVKLALYVSKSKRELLTGKSKPQTFVSKNINYFLFFIPILIIAAGLFINFGDIKL

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Crispr_ID: NZ_CP024870_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP024870_2

1208080-1208191

Orphan

Consensus_repeat	Method
GCTTTTGGTGCGGCTGCTTTTTTAG	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_CP024870_2

>merge|NZ_CP024870|2|1208080-1208191|CRISPRCasFinder
GCAGCTGGTTCGGCTGCTTTTGCAGTAGCTTTTGGTGCGGCTGCTTTTTTAGTTGCTGCTTTTGGGGCAGCAGCTTTTTTAACTTTTGCTTTTGGTGCAGCTGCTTTTTTAG

>NZ_CP024870|2|2|1208080-1208191|CRISPRCasFinder
GCAGCTGGTTCGGCTGCTTTTGCAG	TAGCTTTTGGTGCGGCT
GCTTTTTTAGTTGCTGCTTTTGGGG	CAGCAGCTTTTTTAACTTTT
GCTTTTGGTGCAGCTGCTTTTTTAG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP024870.1\|WP_100254935.1\|1210513_1211239_-\|UMP-kinase	unknown	unknown	gnl\|CDD\|239787
NZ_CP024870.1\|WP_100254938.1\|1213312_1214185_-\|elongation-factor-Ts	unknown	unknown	gnl\|CDD\|236491
NZ_CP024870.1\|WP_100254928.1\|1207319_1207820_-\|16S-rRNA-processing-protein-RimM	unknown	unknown	gnl\|CDD\|274061
NZ_CP024870.1\|WP_100254939.1\|1214202_1215000_-\|30S-ribosomal-protein-S2	unknown	unknown	gnl\|CDD\|235396
NZ_CP024870.1\|WP_100254926.1\|1206215_1206584_-\|50S-ribosomal-protein-L19	unknown	unknown	gnl\|CDD\|235418
NZ_CP024870.1\|WP_100254929.1\|1207806_1208064_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254920.1\|1199586_1200147_-\|SMC-Scp-complex-subunit-ScpB	unknown	unknown	gnl\|CDD\|234657
NZ_CP024870.1\|WP_100254923.1\|1201920_1203000_+\|flavodoxin-dependent-(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate-synthase	unknown	unknown	gnl\|CDD\|377382
NZ_CP024870.1\|WP_100254933.1\|1209686_1209950_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|236800
NZ_CP024870.1\|WP_100254927.1\|1206588_1207320_-\|tRNA-(guanosine(37)-N1)-methyltransferase-TrmD	unknown	unknown	gnl\|CDD\|234581
NZ_CP024870.1\|WP_100254934.1\|1209961_1210510_-\|ribosome-recycling-factor	unknown	unknown	gnl\|CDD\|178850
NZ_CP024870.1\|WP_100254940.1\|1215482_1216145_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254932.1\|1209426_1209702_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254922.1\|1200912_1201761_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254921.1\|1200136_1200901_-\|segregation/condensation-protein-A	unknown	unknown	gnl\|CDD\|234635
NZ_CP024870.1\|WP_100254924.1\|1202989_1204456_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254931.1\|1208597_1209344_-\|Pr6Pr-family-membrane-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254937.1\|1212508_1213300_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP024870.1\|WP_100254925.1\|1204512_1206042_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|379484
NZ_CP024870.1\|WP_100254936.1\|1211384_1212329_-\|energy-coupled-thiamine-transporter-ThiT	unknown	unknown	gnl\|CDD\|370542

Protein	Function_ID	Function_description	E-value
NZ_CP024870.1\|WP_100254935.1\|1210513_1211239_-\|UMP-kinase	gnl\|CDD\|239787	cd04254, AAK_UMPK-PyrH-Ec, UMP kinase (UMPK)-Ec, the microbial/chloroplast uridine monophosphate kinase (uridylate kinase) enzyme that catalyzes UMP phosphorylation and plays a key role in pyrimidine nucleotide biosynthesis; regulation of this process is via feed-back control and via gene repression of carbamoyl phosphate synthetase (the first enzyme of the pyrimidine biosynthesis pathway). The UMP kinase of E. coli (Ec) is known to function as a homohexamer, with GTP and UTP being allosteric effectors. Like other related enzymes (carbamate kinase, aspartokinase, and N-acetylglutamate kinase) the E. coli and most bacterial and chloroplast UMPKs (this CD) have a conserved, N-terminal, lysine residue proposed to function in the catalysis of the phosphoryl group transfer, whereas most archaeal UMPKs appear to lack this residue and the Pyrococcus furiosus structure has an additional Mg ion bound to the ATP molecule which is proposed to function as the catalysis instead. Members of this CD belong to the Amino Acid Kinase Superfamily (AAK).	1.26081e-108
NZ_CP024870.1\|WP_100254928.1\|1207319_1207820_-\|16S-rRNA-processing-protein-RimM	gnl\|CDD\|274061	TIGR02273, Ribosome_maturation_factor_RimM, 16S rRNA processing protein RimM. This family consists of the bacterial protein RimM (YfjA, 21K), a 30S ribosomal subunit-binding protein implicated in 16S ribsomal RNA processing. It has been partially characterized in Escherichia coli, is found with other translation-associated genes such as trmD. It is broadly distributed among bacteria, including some minimal genomes such the aphid endosymbiont Buchnera aphidicola. The protein contains a PRC-barrel domain that it shares with other protein families (pfam05239) and a unique domain (pfam01782). This model describes the full-length protein. A member from Arabidopsis (plant) has additional N-terminal sequence likely to represent a chloroplast transit peptide. [Transcription, RNA processing].	1.28271e-19
NZ_CP024870.1\|WP_100254939.1\|1214202_1215000_-\|30S-ribosomal-protein-S2	gnl\|CDD\|235396	PRK05299, rpsB, 30S ribosomal protein S2; Provisional.	2.06088e-124
NZ_CP024870.1\|WP_100254926.1\|1206215_1206584_-\|50S-ribosomal-protein-L19	gnl\|CDD\|235418	PRK05338, rplS, 50S ribosomal protein L19; Provisional.	7.11767e-58
NZ_CP024870.1\|WP_100254920.1\|1199586_1200147_-\|SMC-Scp-complex-subunit-ScpB	gnl\|CDD\|234657	PRK00135, scpB, segregation and condensation protein B; Reviewed.	3.12276e-54
NZ_CP024870.1\|WP_100254923.1\|1201920_1203000_+\|flavodoxin-dependent-(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate-synthase	gnl\|CDD\|377382	pfam04551, GcpE, GcpE protein. In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. GcpE gene of Escherichia coli is involved in this pathway.	0
NZ_CP024870.1\|WP_100254936.1\|1211384_1212329_-\|energy-coupled-thiamine-transporter-ThiT	gnl\|CDD\|370542	pfam09515, Thia_YuaJ, Thiamine transporter protein (Thia_YuaJ). Members of this protein family have been assigned as thiamine transporters by a phylogenetic analysis of families of genes regulated by the THI element, a broadly conserved RNA secondary structure element through which thiamine pyrophosphate (TPP) levels can regulate transcription of many genes related to thiamine transport, salvage, and de novo biosynthesis. Species with this protein always lack the ThiBPQ ABC transporter. In some species (e.g. Streptococcus mutans and Streptococcus pyogenes), yuaJ is the only THI-regulated gene. Evidence from Bacillus cereus indicates thiamine uptake is coupled to proton translocation.	9.67219e-13
NZ_CP024870.1\|WP_100254927.1\|1206588_1207320_-\|tRNA-(guanosine(37)-N1)-methyltransferase-TrmD	gnl\|CDD\|234581	PRK00026, trmD, tRNA (guanine-N(1)-)-methyltransferase; Reviewed.	8.28121e-125
NZ_CP024870.1\|WP_100254934.1\|1209961_1210510_-\|ribosome-recycling-factor	gnl\|CDD\|178850	PRK00083, frr, ribosome recycling factor; Reviewed.	9.09096e-74
NZ_CP024870.1\|WP_100254933.1\|1209686_1209950_-\|hypothetical-protein	gnl\|CDD\|236800	PRK10935, PRK10935, nitrate/nitrite two-component system sensor histidine kinase NarQ.	0.0027308
NZ_CP024870.1\|WP_100254921.1\|1200136_1200901_-\|segregation/condensation-protein-A	gnl\|CDD\|234635	PRK00104, scpA, segregation and condensation protein A; Reviewed.	4.63935e-50
NZ_CP024870.1\|WP_100254938.1\|1213312_1214185_-\|elongation-factor-Ts	gnl\|CDD\|236491	PRK09377, tsf, elongation factor Ts; Provisional.	3.09368e-134
NZ_CP024870.1\|WP_100254925.1\|1204512_1206042_-\|hypothetical-protein	gnl\|CDD\|379484	pfam14126, DUF4293, Domain of unknown function (DUF4293). This family of integral membrane proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 136 and 154 amino acids in length.	0.00496071

>NZ_CP024870.1|WP_100254929.1|1207806_1208064_-|hypothetical-protein
MDFAFQTIKQILTDVLPESVNYIFQPKAEFEDYYSFILVIDNNAKSIELINKTPLIPTIQNALNLDISTIGKKVEIEVEIFDESA
>NZ_CP024870.1|WP_100254928.1|1207319_1207820_-|16S-rRNA-processing-protein-RimM
MSLLKQLTEIGVLKATHGIKGEFKLWINDDIIFEHSPIGNQLFLQDKKNNFDVYTVEKFYSVKVKQIVKFKEINTIDQAKGLVGQQVFVKTDQNLITVKTTLLGFAVYCQEKLWGHVIELMNNGAYELIKVKNNQVEFWVPCVDKYIVKIDKATNSIYAIDLEMLQ
>NZ_CP024870.1|WP_100254927.1|1206588_1207320_-|tRNA-(guanosine(37)-N1)-methyltransferase-TrmD
MKFSIITLFPNLISSYISESIIKRAIDKQKIEIDIIDLRQHTTYSHNQVDDYQFGGGKGMVLMVEPVVNAINSCKTKDSVVLLTSPQGKTWSQNLVKTFKNNYQHLIIVCGHYEGFDERILDYIDFEVSVGDYVITGGELASLIVVDSITRLIEGVIANESHQNDSFENNLLDHPVYTKPVEFDGKKVPEVLLSGHHANIEKFRQQGRIKNTYDKRPDLLETNNWSADDLDYLKKLQKKLKGD
>NZ_CP024870.1|WP_100254926.1|1206215_1206584_-|50S-ribosomal-protein-L19
MNMLTKKTKEIVDEQLNQNLPDFSSGDTIKVNVKIQEGDNYRIQVFEGVVIKTQGSGISYSVIVRKNSNGVFVERTFLVHSPLIDSIEVVKRGRVRRARIYYIRKLSGKAARIKEIVTKKAK
>NZ_CP024870.1|WP_100254925.1|1204512_1206042_-|hypothetical-protein
MGFKKGALVIYHDQNWVVNNIEDSTTPSGFLTLLTIKNIITNEIRQVRAQDVQKFVVNEKKVNSSDTRIINDLFNDGVSAKDRFHEDSGDFDHFHLNENETSYGVDEIEVEKAPSFETQPLNQPKTQEVFSNIVQERANKKPDYASDFKPLSIVDETISDMTPIGELSTYEFIPTRLKKNTATQTAPQPELITPKSKQVSMTDPTIVVQKPQNSNTIKTERILTGHMTGNSKVKYLDDNVFETAKTQPSIERPKTAPLQSKPEYKNTTQTDKMMLEGITNKFPENDLTKSKTFGPLNTRRLIEEYENQQEFIKNKGNLIDSEEELTGLVYQDANSLKNNLFKNSKIYKTFKKQSLGLILIFVFMLLVPMIGIFMKLATFWLTAKMFNLTILTIINFSLTEVTFSNIIYLVSDILLIIITPILILTFMIYLVCYLVSTNSRQLQKLAMFNQFVRAKDHVIEGIQDYNSQTSRYFVKIHNEMKEIKKEISDLKKVTTADSTEVPKTVKVAH
>NZ_CP024870.1|WP_100254924.1|1202989_1204456_-|hypothetical-protein
MKNWDLQIASPIFLLIAIINLGWIVHKEKIKSKSLICLVEIALFWMLLGYMFFKITQYTQANLDFLYANTNTNSYWIGSLFLGLIFLIGQPLGVFLTTICKNRKIWICSAYALTIMWFVLDSSVTSSLTLNLVLIALFFLISTAVPILVLDINEQYFYKINTYALVWVLISFCATSLLIAHVYYEFDFVYLKKVRWIFPAITGSIAILLLILGFVRRSNQQSIGPFELVIREQLPQINLKTMLFLYSLGFLVTLAFILSNSVFVKAQIITNLTSIGFAEGQISLLNRIGTGWFLIPTFLLTYILNKTIINSIGQKNLIFIALFTLFGLYAGMSFISNGIALLIFSILAGFCYVQIIFSLFSIALFWNCRTVKQPVVGYFCSASYFAVFLVKLINGLLLASKSDLMQAIGLQENLINYQEQINEIGSIILACAASVVLVAAVVFNFSSKYLLADFRNYKFAIQNLKAIIKNSIKQKTKTQIDFKNQTTD
>NZ_CP024870.1|WP_100254923.1|1201920_1203000_+|flavodoxin-dependent-(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate-synthase
MTHRKNTRAVFVNDIQIGGNNQVIIQSMTTTKTHDVAATLTQINQLHKEGCAIVRVAVLGINDANALSEIVAQSPVPIVADIHFNYKFALIAADAGCAKIRINPGNIGIEENTIAVVEKCKEKQIPIRIGINSGSLPKKLVAKYGWTAECMVEALREHIEILEKYDFYDIIYSLKSTDPLMAIEAYTLASKNWDYPAHLGITEAGSLLNGAIKSSFGIGHLLYQGIGSTIRISLSEDPVMEIKVAKRLLNAMGLYENLVEVIACPTCGRLEFGLAKVVQEIEEFVEDLHFPLKIAILGCVVNGPGEAAQADIGIAGGKDGGIIFKKGKLFKTAKQAELIPELKALILEYFEIWKNQTNQ
>NZ_CP024870.1|WP_100254922.1|1200912_1201761_-|hypothetical-protein
MSVKITLRDIVEINKVLTQKNYASQVEFNAYLDVIGDYLDVTFFENSAITEKLTQYAEQSERNLDMKFFVKTDVDLSVNQLNDYMLNCKRALEKALYGDWTTFKFYIFAEVKSIVRYYLEKTYEYEALMDFETLYGIKTIEFHQQNETFKYLYSVFDKFTYIARYLNDRYVKNQKNDLNELTLKFYNDFVNYINFLTKDEEANNVLKSTLDKITSSKAWHFIRRLRNNLEHDFSNPSRKYNISFSLQLLFIIIGRIMLVLRKTLKTDLEMKQIFEVLKNKEK
>NZ_CP024870.1|WP_100254921.1|1200136_1200901_-|segregation/condensation-protein-A
MERWQELKLHNFTGPLDLLLYMIKEKQLSIFEVNLLELSNQYLAYINSLSQLDIEIASEYLIMAAYLIELKSKLLIPKLEVEIDSNYEADQREELLARLLEYHKIKEVTAFFKEQQLEGLKLYSKPKSVFKITKIDDDSLPLAPAHIDIDKFAQIFLKVIEKNRFKTMQTNTVTSVEISPEEVARDIQQYLEEHKIEKISLEDLIAEKEFSIRMLVATFLAILDLAAKGKIDIFQADDHVMVQNLLKEEITNGI
>NZ_CP024870.1|WP_100254920.1|1199586_1200147_-|SMC-Scp-complex-subunit-ScpB
MEFKKQVAVTEGLLFVNGDEGVSIDDLKFILETNDETLINQIINTLIENYENNEMSGLTIQKFNKVKFRLVTKRENAEYFQKLSNVKTETRLSTASIETVSIIAYRGPITKAQVEELRGVNCDTIFYKLKLRNLIQEVGKSEEVGKPTLYNITEDFLKYFNLSSLDDLPKLKEIIEEDEKDIFNRG
>NZ_CP024870.1|WP_100254931.1|1208597_1209344_-|Pr6Pr-family-membrane-protein
MNKIRMAYKLTFAIWGLASILAAYLFKIIDGTEIKTTYLGNYEIFTIDFLSTFTILSNFLVLFWLLFAAIFYKKEGEAKLLSQTSGLIASVYISVTLVIYNFVLVPLDSAFPKDSFNQYVSIMNHVINPLAFVFYFLVLMPKKEHINLNEFFLKRFYIIFCLILAYCIYAMLRGEMRFQSSGDLYESHNLFYPYFFLNVHKTGPLNLAGGVWFAIAFFAIIACLIGFCYLYNFSSNLIIKTKFYQNLN
>NZ_CP024870.1|WP_100254932.1|1209426_1209702_-|hypothetical-protein
MDTSLKEVCYYCKEVLLENQPTIKIKIIETGPLNLYHLECGKKRKKDYKYTLLATNSKPQSKNWYFKTIIALFAVFVIEMIILITLIVIKS
>NZ_CP024870.1|WP_100254933.1|1209686_1209950_-|hypothetical-protein
MICKICKQVISQEQEFVKDYFDEKVETYHCSCHLEQFGDIKEIEEANTLQYKKTYEQNVGQFSNFVFLIILAVSIILGLVGIIWILV
>NZ_CP024870.1|WP_100254934.1|1209961_1210510_-|ribosome-recycling-factor
MINVLLEQTKKEMQGTVAAFEDYLTKVRTGRANANMLKGVMVDFYGTPTPIDQTSQISAPEPQQLVIKPYDPSQIGVIIAGINKADLGLHSLAEANLIRINIPALTEEVRKDLVKKMSKELESFKVRIRNTRRDVNEKIKKSSDLAEDIKKDGENSVQKLTDEFIAKLDAMAKAKEKDLMTI
>NZ_CP024870.1|WP_100254935.1|1210513_1211239_-|UMP-kinase
MELKFKRVLLKISGEALKSGADDIYEKDKLDDVARQIIELTNEGLQIGLVIGGGNIWRGKLAGSLGLHRIEADYMGMLATIMNALALEASLKKMDFDKVKVYSSLEIKTVTTAYNYREAREKLEQGYVTIFAGGTGFSYFTTDTGASIRAIEIEADALLMAKNGTKGVYDSDPNINPKAKFYKHLSFDDLVSKKLQVMDSTAATLSRDGNLNVVVFDMNGHNNIKKMAYGELEATYINTEE
>NZ_CP024870.1|WP_100254936.1|1211384_1212329_-|energy-coupled-thiamine-transporter-ThiT
MKKNTWNILTITLASLRSILLLGLLTWLAIVVASKNKVDANPLTANEKTLIIFCFIFAFLAFSLLTTNIVLLFIYDLNVFNIKVVILAVITLNIELLVYLVINWAEVFRTIRWSKPQKWSIFDITTMGLLLGIYLIIDYVSSFVPVMPFWITPSFKYVVLFFAAYILTFSCTFSLCIIIGFITIIMPGTAVMSPVQYIFDYFVPIMAFSLASFFRPIEKIDNKVVQILQWNLYVFVPMLIVYLSRVISGIAFWLNPAVYDGVSYEFNWNGAFTYSLIFNAFNSIVDYFVLVILTPMICIPLTVIKHKQEINRQQ
>NZ_CP024870.1|WP_100254937.1|1212508_1213300_-|hypothetical-protein
MIFANYDFIDGALSKDGALFTNFVILLGIGLVVTLIPTLYALYLFVGFKKSEGKKFLSKPWFAGILIAEVLSIIFCCLGWMFFFGEFILGTGSEAGNKAFTLVLTFTILTYVTAVGWYAILLFWAHYIWIQIEENQIVTISSTIKKEKIVKVIKDENKGKVYINFTESKTSLKKLSFRIGSTMTQFIIENLSASGIKVEVGNQEEFYQNKVVELKSVPAEKLRTAASKKDDSKAKPKSESAKTESEPKTKTEPETKTSEDTEK
>NZ_CP024870.1|WP_100254938.1|1213312_1214185_-|elongation-factor-Ts
MAVTPQLIKELREMTSAGMMDCKKALEATNGNIDDAVVWLRENGLAKAAKKADRVAAEGVAFAKSNGKRGIIFEINSETDFVSKNEKFMTLVNEIGEALLESNAKTLEEALKVKLKSGETVEQACISATATIGEKIEVRRFDVVDGNTISFYNHANSRISVLLQFSGNIAEEDAYNLCMHVAAMAPKYLSKDDVPEDFKATEMHIIKETTDLTGKPEKVAEGILQGKLSKKLAEVTLLEQGFVMDEKVKVSEFVKQKGASLVTMYRFEVGEGIEKVVTDFAAEVAAQLKG
>NZ_CP024870.1|WP_100254939.1|1214202_1215000_-|30S-ribosomal-protein-S2
MAKDLTRDQLWDAGAHYGHQTKRWNPKMKPYIYGAKNKNHIIDLQQTLWRLEDVKKFVTSIGEKKGKIIFVGTKRSSKNAVKEAALRSQNFYINSRWLGGTLTNMKTISSRIKTLLDIEIEEKTGKINLRPKKEQIKIKKEKAKLEKTLGGIKHMRKLPNAMFVVDPKTDEIAVKEARKLNIPIIAICDTNVDPDMVDYIIPANDDIQESVNLITNFIVDVYADAAGLKLQPSNLKVVAQKKEERPAGGPRNYTPNSDSKLVDTK
>NZ_CP024870.1|WP_100254940.1|1215482_1216145_+|hypothetical-protein
MKKINQKNILFTVIFILNNLLVTFVLFKILTQHFLDMDFKDEIDYQLAWLGFFTLALLMNFLELAFPKIPRFCFFISYWFFPISLPYLILAYQVAKKKITVDQETIDCYYKCSTKIAFIVSLLIFSFTFVWLFNSWAIIILFLFDEHRPKSLFVYTIISIYFLGNIIALCLNFGNNKTFKKIAWTGFMAGWSYLVFEKLTLTSLKNNVNLVESDAEKNLT

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	MN693496	Marine virus AFVG_25M103, complete genome	43562-43592	5	0.839
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	MK448428	Streptococcus satellite phage Javan322, complete genome	4882-4910	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP053970	Bacillus thuringiensis strain FDAARGOS_796 plasmid unnamed2, complete sequence	269546-269574	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP053979	Bacillus thuringiensis strain FDAARGOS_795 plasmid unnamed3, complete sequence	177758-177786	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP013277	Bacillus thuringiensis serovar israelensis strain AM65-52 plasmid pAM65-52-2-350K, complete sequence	46480-46508	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP039723	Bacillus thuringiensis strain BT-59 plasmid p2, complete sequence	163481-163509	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP009348	Bacillus thuringiensis HD1002 plasmid 2, complete sequence	46480-46508	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP045024	Bacillus thuringiensis strain JW-1 plasmid p2, complete sequence	46480-46508	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NC_018516	Bacillus thuringiensis HD-789 plasmid pBTHD789-1, complete sequence	160903-160931	6	0.793
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	NZ_CP009334	Bacillus thuringiensis strain HD1011 plasmid 2, complete sequence	95100-95128	6	0.793
NZ_CP024870_1	1.6\|958163\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958163-958192	30	MH319759	Marine virus AG-345-O18 Ga0172273_13 genomic sequence	16541-16570	6	0.8
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NC_041878	Pectobacterium phage CBB, complete genome	120458-120488	6	0.806
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NC_019401	Cronobacter phage vB_CsaM_GAP32, complete genome	124178-124208	6	0.806
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	AP013483	Uncultured Mediterranean phage uvMED DNA, complete genome, group G19, isolate: uvMED-CGR-C20A-MedDCM-OCT-S30-C71	19910-19938	7	0.759
NZ_CP024870_1	1.1\|957833\|29\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	957833-957861	29	AP013484	Uncultured Mediterranean phage uvMED DNA, complete genome, group G19, isolate: uvMED-CGR-C20A-MedDCM-OCT-S36-C79	12045-12073	7	0.759
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NZ_CP013139	Pseudoalteromonas sp. Bsw20308 plasmid pPBSW1, complete sequence	324780-324810	7	0.774
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NC_041831	Campylobacter phage vB_CcoM-IBB_35 clone Contig3, complete sequence	12123-12153	7	0.774
NZ_CP024870_1	1.8\|958296\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958296-958325	30	MN840487	Proteus phage 2207-N35, complete genome	34806-34835	7	0.767
NZ_CP024870_1	1.12\|958561\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958561-958590	30	AP014314	Uncultured Mediterranean phage uvMED isolate uvMED-GF-U-MedDCM-OCT-S31-C24, * SEQUENCING IN PROGRESS *	20813-20842	7	0.767
NZ_CP024870_1	1.12\|958561\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958561-958590	30	LT960552	Yersinia phage fHe-Yen9-03 genome assembly, complete genome: monopartite	162675-162704	7	0.767
NZ_CP024870_1	1.12\|958561\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958561-958590	30	LR596615	Yersinia phage fHe-Yen9-04 genome assembly, chromosome: I	162287-162316	7	0.767
NZ_CP024870_1	1.12\|958561\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958561-958590	30	NC_042116	Yersinia phage fHe-Yen9-04 genome assembly, complete genome: monopartite	162287-162316	7	0.767
NZ_CP024870_1	1.13\|958627\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958627-958656	30	NZ_CP020413	Leptospira interrogans serovar Copenhageni strain FDAARGOS_203 plasmid unnamed1, complete sequence	201240-201269	7	0.767
NZ_CP024870_1	1.6\|958163\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958163-958192	30	MN856091	Myoviridae sp. isolate 9, partial genome	17093-17122	8	0.733
NZ_CP024870_1	1.6\|958163\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958163-958192	30	MN856055	Myoviridae sp. isolate 330, complete genome	40578-40607	8	0.733
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NZ_LR214939	Mycoplasma salivarium strain NCTC10113 plasmid 2	552523-552553	8	0.742
NZ_CP024870_1	1.8\|958296\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958296-958325	30	MH707430	Bacillus phage BSP7, complete genome	40528-40557	8	0.733
NZ_CP024870_1	1.10\|958428\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958428-958458	31	AP014501	Uncultured Mediterranean phage uvMED isolate uvMED-GF-U-MedDCM-OCT-S32-C4, * SEQUENCING IN PROGRESS *, 3 ordered pieces	1768-1798	8	0.742
NZ_CP024870_1	1.13\|958627\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958627-958656	30	NZ_CP048789	Roseobacter ponti strain DSM 106830 plasmid p1, complete sequence	52045-52074	8	0.733
NZ_CP024870_1	1.13\|958627\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958627-958656	30	NZ_LR699555	Paraburkholderia sp. Msb3 isolate PDMSB31 plasmid pI	77864-77893	8	0.733
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NZ_LR215032	Mycoplasma gallopavonis strain NCTC10186 plasmid 2	251318-251348	9	0.71
NZ_CP024870_1	1.7\|958229\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958229-958259	31	NZ_CP022991	Paraburkholderia aromaticivorans strain BN5 plasmid pBN1, complete sequence	137044-137074	9	0.71
NZ_CP024870_1	1.10\|958428\|31\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958428-958458	31	MN693453	Marine virus AFVG_25M364, complete genome	3388-3418	9	0.71
NZ_CP024870_1	1.15\|958759\|30\|NZ_CP024870\|PILER-CR,CRISPRCasFinder,CRT	958759-958788	30	NZ_CP022057	Staphylococcus saprophyticus strain FDAARGOS_336 plasmid unnamed2, complete sequence	17500-17529	9	0.7

1. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MN693496 (Marine virus AFVG_25M103, complete genome) position: , mismatch: 5, identity: 0.839

tttgctg-aaatttggatttttgattttaaag	CRISPR spacer
-ttgcagtaaatttagatttatgattttaaac	Protospacer
 **** * ******.***** **********

2. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MK448428 (Streptococcus satellite phage Javan322, complete genome) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
agcaagttagaaaggagaaacttcattgc	Protospacer
*.  ****************** ** **

3. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP053970 (Bacillus thuringiensis strain FDAARGOS_796 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

4. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP053979 (Bacillus thuringiensis strain FDAARGOS_795 plasmid unnamed3, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

5. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013277 (Bacillus thuringiensis serovar israelensis strain AM65-52 plasmid pAM65-52-2-350K, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

6. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP039723 (Bacillus thuringiensis strain BT-59 plasmid p2, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

7. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP009348 (Bacillus thuringiensis HD1002 plasmid 2, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

8. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP045024 (Bacillus thuringiensis strain JW-1 plasmid p2, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

9. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NC_018516 (Bacillus thuringiensis HD-789 plasmid pBTHD789-1, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

10. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP009334 (Bacillus thuringiensis strain HD1011 plasmid 2, complete sequence) position: , mismatch: 6, identity: 0.793

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
aaataagtagaaaggagaaactatagaga	Protospacer
*****. ****************.*. *.

11. spacer 1.6|958163|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MH319759 (Marine virus AG-345-O18 Ga0172273_13 genomic sequence) position: , mismatch: 6, identity: 0.8

tgcacttgtgtttaatgcaagttttaatga	CRISPR spacer
tcctcttttgtttaatacaagttttaatct	Protospacer
* * *** ********.***********

12. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NC_041878 (Pectobacterium phage CBB, complete genome) position: , mismatch: 6, identity: 0.806

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
tgtggcgaaatttggatgttcgattttaaat	Protospacer
* ** .*********** **.*********

13. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NC_019401 (Cronobacter phage vB_CsaM_GAP32, complete genome) position: , mismatch: 6, identity: 0.806

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
tgtggcgaaatttggatgttcgattttaaat	Protospacer
* ** .*********** **.*********

14. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to AP013483 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G19, isolate: uvMED-CGR-C20A-MedDCM-OCT-S30-C71) position: , mismatch: 7, identity: 0.759

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
ataataataaaaaggagaaactacaatgt	Protospacer
* *  . **.******************

15. spacer 1.1|957833|29|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to AP013484 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G19, isolate: uvMED-CGR-C20A-MedDCM-OCT-S36-C79) position: , mismatch: 7, identity: 0.759

aaatagttagaaaggagaaactacaatgg	CRISPR spacer
ataataataaaaaggagaaactacaatgt	Protospacer
* *  . **.******************

16. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013139 (Pseudoalteromonas sp. Bsw20308 plasmid pPBSW1, complete sequence) position: , mismatch: 7, identity: 0.774

tttgctgaaatttggatttttgattttaaag---	CRISPR spacer
tttgctgaaatttggtttttagacc---aagtta	Protospacer
*************** **** **..   ***

17. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NC_041831 (Campylobacter phage vB_CcoM-IBB_35 clone Contig3, complete sequence) position: , mismatch: 7, identity: 0.774

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
ggtgatttaattttgattttagattttaaag	Protospacer
  ** *  ***** ****** **********

18. spacer 1.8|958296|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MN840487 (Proteus phage 2207-N35, complete genome) position: , mismatch: 7, identity: 0.767

gcaattatgaacaaggtgagggagtgtatg	CRISPR spacer
gtaattgtgaccaaggtgagggagtacctt	Protospacer
*.****.*** **************.. *

19. spacer 1.12|958561|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to AP014314 (Uncultured Mediterranean phage uvMED isolate uvMED-GF-U-MedDCM-OCT-S31-C24, *** SEQUENCING IN PROGRESS ***) position: , mismatch: 7, identity: 0.767

atgaatgaagtatgattaactacttaagtg	CRISPR spacer
gtcaatgaacgatgattaactacttagatt	Protospacer
.* ******  ***************..*

20. spacer 1.12|958561|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to LT960552 (Yersinia phage fHe-Yen9-03 genome assembly, complete genome: monopartite) position: , mismatch: 7, identity: 0.767

---atgaatgaagtatgattaactacttaagtg	CRISPR spacer
tacgtga---aagtatggttaactacttaagat	Protospacer
   .***   *******.*************

21. spacer 1.12|958561|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to LR596615 (Yersinia phage fHe-Yen9-04 genome assembly, chromosome: I) position: , mismatch: 7, identity: 0.767

---atgaatgaagtatgattaactacttaagtg	CRISPR spacer
tacgtga---aagtatggttaactacttaagat	Protospacer
   .***   *******.*************

22. spacer 1.12|958561|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NC_042116 (Yersinia phage fHe-Yen9-04 genome assembly, complete genome: monopartite) position: , mismatch: 7, identity: 0.767

---atgaatgaagtatgattaactacttaagtg	CRISPR spacer
tacgtga---aagtatggttaactacttaagat	Protospacer
   .***   *******.*************

23. spacer 1.13|958627|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP020413 (Leptospira interrogans serovar Copenhageni strain FDAARGOS_203 plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.767

ttcttttcttttctcggaaatggtgcctta	CRISPR spacer
ttcttttcttttgtcggacatggtaataga	Protospacer
************ ***** *****. .  *

24. spacer 1.6|958163|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MN856091 (Myoviridae sp. isolate 9, partial genome) position: , mismatch: 8, identity: 0.733

tgcacttgtgtttaatgcaagttttaatga	CRISPR spacer
attatttttgtttaatgcaagttttatttg	Protospacer
  .*.** ****************** * .

25. spacer 1.6|958163|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MN856055 (Myoviridae sp. isolate 330, complete genome) position: , mismatch: 8, identity: 0.733

tgcacttgtgtttaatgcaagttttaatga	CRISPR spacer
attatttttgtttaatgcaagttttatttg	Protospacer
  .*.** ****************** * .

26. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_LR214939 (Mycoplasma salivarium strain NCTC10113 plasmid 2) position: , mismatch: 8, identity: 0.742

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
aatggcaaaagtttgatttttgattttaaaa	Protospacer
  ** ..*** ** ****************.

27. spacer 1.8|958296|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MH707430 (Bacillus phage BSP7, complete genome) position: , mismatch: 8, identity: 0.733

gcaattatgaacaaggtgagggagtgtatg	CRISPR spacer
tcaattatgaacatggtgatggagacattc	Protospacer
 ************ ***** ****    *

28. spacer 1.10|958428|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to AP014501 (Uncultured Mediterranean phage uvMED isolate uvMED-GF-U-MedDCM-OCT-S32-C4, *** SEQUENCING IN PROGRESS ***, 3 ordered pieces) position: , mismatch: 8, identity: 0.742

tagact---taacaggtacctttggtagtttaaa	CRISPR spacer
---attttataacaggtatctctggtagtttatc	Protospacer
   *.*   *********.**.**********

29. spacer 1.13|958627|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP048789 (Roseobacter ponti strain DSM 106830 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

ttcttttcttttctcggaaatggtgcctta	CRISPR spacer
gatggtgcttttctcggaactggcgcctta	Protospacer
  .  * ************ ***.******

30. spacer 1.13|958627|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_LR699555 (Paraburkholderia sp. Msb3 isolate PDMSB31 plasmid pI) position: , mismatch: 8, identity: 0.733

ttcttttcttttctcggaaatggtgcctta	CRISPR spacer
gaatttgctgttctcggaaatggtgcagaa	Protospacer
   *** ** ****************   *

31. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_LR215032 (Mycoplasma gallopavonis strain NCTC10186 plasmid 2) position: , mismatch: 9, identity: 0.71

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
gaacttcaaattttgaattttgattttaaaa	Protospacer
    .* ****** ** *************.

32. spacer 1.7|958229|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP022991 (Paraburkholderia aromaticivorans strain BN5 plasmid pBN1, complete sequence) position: , mismatch: 9, identity: 0.71

tttgctgaaatttggatttttgattttaaag	CRISPR spacer
gcaaatgaaatttggattttagattgtagcg	Protospacer
 . . *************** **** **. *

33. spacer 1.10|958428|31|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to MN693453 (Marine virus AFVG_25M364, complete genome) position: , mismatch: 9, identity: 0.71

tagacttaacaggtacctttggtagtttaaa	CRISPR spacer
gtgagttaacaggtaactttggtagcgagga	Protospacer
  ** ********** *********.  ..*

34. spacer 1.15|958759|30|NZ_CP024870|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP022057 (Staphylococcus saprophyticus strain FDAARGOS_336 plasmid unnamed2, complete sequence) position: , mismatch: 9, identity: 0.7

gcacttgagcacaattgaatttgataaaac	CRISPR spacer
attaccgagcacacttgaatttgctaaaaa	Protospacer
..  ..******* ********* *****

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

786686 : 843199

Bacillus_phage(22.22%)

integrase,tRNA,transposase

attL 787410:787469|attR 843200:843705

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_146637825.1\|786686_787409_-\|integrase,transposase	DDE-type integrase/transposase/recombinase	NA	NA	NA	NA
787410:787469	attL	NA	NA	NA	NA
WP_100254044.1\|787534_787831_-\|transposase	transposase	NA	NA	NA	NA
WP_100254554.1\|787985_788648_-	deoxyribose-phosphate aldolase	NA	NA	NA	NA
WP_100254555.1\|788651_789638_-	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_100254556.1\|789641_790214_-	hypothetical protein	NA	NA	NA	NA
WP_100254557.1\|790302_791538_-	motility-associated protein Scm1	NA	NA	NA	NA
WP_100254558.1\|791566_793798_-	AAA family ATPase	A0A2K9V9X6	Bandra_megavirus	1.8e-36	26.7
WP_100254559.1\|793946_796565_-	DNA topoisomerase IV subunit A	A0A172JHV7	Bacillus_phage	4.3e-101	32.5
WP_100254560.1\|796582_797536_-	hypothetical protein	NA	NA	NA	NA
WP_100254561.1\|797559_799500_-	DNA topoisomerase IV subunit B	G3M9Z3	Bacillus_virus	5.5e-130	43.7
WP_100254562.1\|799614_800241_-	uridine kinase	A0A1V0SD85	Indivirus	1.7e-24	31.1
WP_146637825.1\|800348_801071_-\|integrase,transposase	DDE-type integrase/transposase/recombinase	NA	NA	NA	NA
WP_100254044.1\|801196_801493_-\|transposase	transposase	NA	NA	NA	NA
WP_100254563.1\|801723_802323_+	FMN-dependent NADH-azoreductase	NA	NA	NA	NA
WP_100254564.1\|802404_802599_+	hypothetical protein	NA	NA	NA	NA
WP_100254565.1\|802629_803934_-	hypothetical protein	NA	NA	NA	NA
WP_100254566.1\|804096_804411_-	MarR family transcriptional regulator	NA	NA	NA	NA
WP_100254567.1\|804540_806505_+	ABC transporter ATP-binding protein/permease	W8CYL7	Bacillus_phage	2.8e-44	30.9
WP_100254568.1\|806577_807483_-	carbohydrate kinase	NA	NA	NA	NA
WP_100254569.1\|807507_808938_-	GH32 C-terminal domain-containing protein	NA	NA	NA	NA
WP_100254570.1\|808959_810483_-	PTS transporter subunit EIIB	NA	NA	NA	NA
WP_169921844.1\|810639_811338_-	DeoR/GlpR transcriptional regulator	NA	NA	NA	NA
WP_100254572.1\|811348_812275_-	carbohydrate kinase family protein	NA	NA	NA	NA
WP_100254573.1\|813148_814297_-	hypothetical protein	NA	NA	NA	NA
WP_100254574.1\|814495_815197_-	hypothetical protein	NA	NA	NA	NA
WP_100254575.1\|815260_815956_+	hypothetical protein	NA	NA	NA	NA
WP_100254576.1\|815984_817712_-\|tRNA	aspartate--tRNA ligase	A0A2K9L424	Tupanvirus	2.3e-18	26.1
WP_100254577.1\|817713_818952_-\|tRNA	histidine--tRNA ligase	A0A1V0SLE3	Klosneuvirus	4.2e-22	27.0
WP_100254578.1\|819065_820001_-	hypothetical protein	NA	NA	NA	NA
WP_100254579.1\|820154_821843_-	hypothetical protein	NA	NA	NA	NA
WP_157795138.1\|821889_822027_-	hypothetical protein	NA	NA	NA	NA
WP_100254580.1\|822259_824632_-	PTS sugar transporter subunit IIA	NA	NA	NA	NA
WP_100254581.1\|824644_825583_-	1-phosphofructokinase	NA	NA	NA	NA
WP_100254582.1\|825586_826291_-	DeoR/GlpR transcriptional regulator	NA	NA	NA	NA
WP_100254583.1\|826486_827302_-	hypothetical protein	NA	NA	NA	NA
WP_100254584.1\|827498_829808_-	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	A0A2H4IAY1	Erwinia_phage	4.9e-08	38.1
WP_100254585.1\|829913_830426_-	adenine phosphoribosyltransferase	A0A2K9L0U5	Tupanvirus	1.0e-06	27.4
WP_169921845.1\|830446_834094_-	protein translocase	NA	NA	NA	NA
WP_100254587.1\|834199_834478_-	hypothetical protein	NA	NA	NA	NA
WP_100254164.1\|834640_836041_+\|integrase,transposase	DDE-type integrase/transposase/recombinase	NA	NA	NA	NA
WP_100254588.1\|836164_837853_-	YitT family protein	NA	NA	NA	NA
WP_100254589.1\|837940_838288_-	hypothetical protein	NA	NA	NA	NA
WP_100254590.1\|838410_839949_-	cytoskeletal motor fibril protein Fib	NA	NA	NA	NA
WP_100254591.1\|840290_842300_-	lipoprotein	NA	NA	NA	NA
WP_146637825.1\|842476_843199_-\|integrase,transposase	DDE-type integrase/transposase/recombinase	NA	NA	NA	NA
843200:843705	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP024870

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection