CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_AP018042	Labilibaculum antarcticum strain SPP2	13 crisprs	csa3,DEDDh,Csx27,cas13b,RT,WYL,cas3	11	6	3	0

1. NZ_AP018042

CRISPR-Cas detection and classification

Crispr_ID: NZ_AP018042_2

• CRISPR_ID: NZ_AP018042_2
• CRISPR_location: 338669-338733
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_2

Consensus_repeat	Method
TTTGGACTTTGGACTTTTGGACTTT	CRISPRCasFinder

spacers of NZ_AP018042_2

>2.1|338694|15|NZ_AP018042|CRISPRCasFinder
TTGACTTTTTGACTT

CRISPR arrays and Neighbor proteins around NZ_AP018042_2

CRISPR arrays

The CRISPR arrays of NZ_AP018042_2

>merge|NZ_AP018042|2|338669-338733|CRISPRCasFinder
ACAGGACTTTCGACTTTTCGACTTTTTGACTTTTTGACTTTTTGACTTTTTGACTTTTTGACTTT

>NZ_AP018042|2|2|338669-338733|CRISPRCasFinder
ACAGGACTTTCGACTTTTCGACTTT	TTGACTTTTTGACTT
TTTGACTTTTTGACTTTTTGACTTT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096427582.1|340858_342046_-|SPOR-domain-containing-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase	unknown	unknown	gnl|CDD|133463
NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase	unknown	unknown	gnl|CDD|187564
NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase	unknown	unknown	gnl|CDD|177986
NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein	unknown	unknown	gnl|CDD|274132
NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein	unknown	unknown	gnl|CDD|370595
NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein	unknown	unknown	gnl|CDD|275083
NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase	unknown	unknown	gnl|CDD|234666
NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein	unknown	unknown	gnl|CDD|293742
NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein	unknown	unknown	gnl|CDD|143586
NZ_AP018042.1|WP_145957556.1|345027_345306_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein	unknown	unknown	gnl|CDD|153248
NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein	unknown	unknown	gnl|CDD|132073
NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter	unknown	unknown	gnl|CDD|273358
NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein	unknown	unknown	gnl|CDD|234874
NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ	unknown	unknown	gnl|CDD|213994
NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein	unknown	unknown	gnl|CDD|275083
NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein	unknown	unknown	gnl|CDD|240532
NZ_AP018042.1|WP_096427574.1|326858_327959_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein	unknown	unknown	gnl|CDD|226288

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase	gnl|CDD|133463	cd06853, GT_WecA_like, This subfamily contains Escherichia coli WecA, Bacillus subtilis TagO and related proteins. WecA is an UDP-N-acetylglucosamine (GlcNAc):undecaprenyl-phosphate (Und-P) GlcNAc-1-phosphate transferase that catalyzes the formation of a phosphodiester bond between a membrane-associated undecaprenyl-phosphate molecule and N-acetylglucosamine 1-phosphate, which is usually donated by a soluble UDP-N-acetylglucosamine precursor. WecA participates in the biosynthesis of O antigen LPS in many enteric bacteria and is also involved in the biosynthesis of enterobacterial common antigen. A conserved short sequence motif and a conserved arginine at a cytosolic loop of this integral membrane protein were shown to be critical in recognition of substrate UDP-N-acetylglucosamine.	5.93933e-85
NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase	gnl|CDD|187564	cd05254, dTDP_HR_like_SDR_e, dTDP-6-deoxy-L-lyxo-4-hexulose reductase and related proteins, extended (e) SDRs. dTDP-6-deoxy-L-lyxo-4-hexulose reductase, an extended SDR, synthesizes dTDP-L-rhamnose from alpha-D-glucose-1-phosphate, providing the precursor of L-rhamnose, an essential cell wall component of many pathogenic bacteria. This subgroup has the characteristic active site tetrad and NADP-binding motif. This subgroup also contains human MAT2B, the regulatory subunit of methionine adenosyltransferase (MAT); MAT catalyzes S-adenosylmethionine synthesis. The human gene encoding MAT2B encodes two major splicing variants which are induced in human cell liver cancer and regulate HuR, an mRNA-binding protein which stabilizes the mRNA of several cyclins, to affect cell proliferation. Both MAT2B variants include this extended SDR domain. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	9.05753e-60
NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase	gnl|CDD|177986	PLN02353, PLN02353, probable UDP-glucose 6-dehydrogenase.	0
NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein	gnl|CDD|293742	cd16018, Enpp, Ectonucleotide pyrophosphatase/phosphodiesterase, also called autotaxin. Ecto-nucleotide pyrophosphatases/phosphodiesterases (ENPPs) hydrolyze 5'-phosphodiester bonds in nucleotides and their derivatives, resulting in the release of 5'-nucleotide monophosphates. ENPPs have multiple physiological roles, including nucleotide recycling, modulation of purinergic receptor signaling, regulation of extracellular pyrophosphate levels, stimulation of cell motility, and possible roles in regulation of insulin receptor (IR) signaling and activity of ecto-kinases. The eukaryotic ENPP family contains at least five members that have different tissue distribution and physiological roles.	2.34552e-08
NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein	gnl|CDD|370595	pfam09640, DUF2027, Domain of unknown function (DUF2027). This protein domain is of unknown function. though putatively involved in DNA mismatch repair. It is associated with pfam01713.	3.59492e-28
NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein	gnl|CDD|275083	TIGR04256, conserved_hypothetical_protein, GxxExxY protein. Members of this protein family average about 130 residues in length and include an almost perfectly conserved motif GxxExxY. Members occur in a wide range of prokaryotes, including Proteobacteria, Perrucomicrobia, Cyanobacteria, Bacteriodetes, Archaea, etc.	4.86973e-53
NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase	gnl|CDD|234666	PRK00147, queA, S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Provisional.	2.54275e-133
NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein	gnl|CDD|274132	TIGR02436, S23_ribosomal_protein, four helix bundle protein. This family describes a protein of unknown function whose structure is a bundle of four long alpha helices. Some of the first members of this family were found encoded in the (atypically large) intervening sequence (IVS) of Leptospira 23S RNA, a region often present in the rRNA gene and removed during rRNA processing without re-ligation. However, this location is not conserved, and naming this protein as a 23S RNA protein is both confusing and inaccurate.	2.4894e-23
NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein	gnl|CDD|143586	cd07185, OmpA_C-like, Peptidoglycan binding domains similar to the C-terminal domain of outer-membrane protein OmpA. OmpA-like domains (named after the C-terminal domain of Escherichia coli OmpA protein) have been shown to non-covalently associate with peptidoglycan, a network of glycan chains composed of disaccharides, which are crosslinked via short peptide bridges. Well-studied members of this family include the Escherichia coli outer membrane protein OmpA, the Escherichia coli lipoprotein PAL, Neisseria meningitdis RmpM, which interact with the outer membrane, as well as the Escherichia coli motor protein MotB, and the Vibrio flagellar motor proteins PomB and MotY, which interact with the inner membrane.	1.73983e-18
NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein	gnl|CDD|153248	cd07986, LPLAT_ACT14924-like, Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: Unknown ACT14924. Lysophospholipid acyltransferase (LPLAT) superfamily member: acyltransferases of de novo and remodeling pathways of glycerophospholipid biosynthesis which catalyze the incorporation of an acyl group from either acylCoAs or acyl-acyl carrier proteins (acylACPs) into acceptors such as glycerol 3-phosphate, dihydroxyacetone phosphate or lyso-phosphatidic acid. Included in this subgroup are uncharacterized phospholipid/glycerol acyltransferases such as the Pectobacterium carotovorum subsp. carotovorum PC1 locus ACT14924 putative acyltransferase, and similar proteins.	3.91246e-82
NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein	gnl|CDD|132073	TIGR03028, EpsE, polysaccharide export protein EpsE. Sequences in this family of proteins are members of a polysaccharide export protein family (pfam02563) which includes the wza protein from E.coli. This family of proteins are homologous to the EpsE protein of the methanolan biosynthesis operon of Methylobacillus species strain 12S. The distribution of this protein appears to be restricted to a subset of exopolysaccharide operons containing a syntenic grouping of genes including a variant of the EpsH exosortase protein. Exosortase has been proposed to be involved in the targetting and processing of proteins containing the PEP-CTERM domain to the exopolysaccharide layer.	1.08433e-20
NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter	gnl|CDD|273358	TIGR00949, Uncharacterized_membrane_protein_YahN, The Resistance to Homoserine/Threonine (RhtB) Family protein. [Transport and binding proteins, Amino acids, peptides and amines].	3.75241e-11
NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein	gnl|CDD|234874	PRK00955, PRK00955, YgiQ family radical SAM protein.	0
NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ	gnl|CDD|213994	cd12110, PHP_HisPPase_Hisj_like, Polymerase and Histidinol Phosphatase domain of Histidinol phosphate phosphatase of Hisj like. Bacillus subtilis YtvP HisJ has strong histidinol phosphate phosphatase (HisPPase) activity. The PHP (also called histidinol phosphatase-2/HIS2) domain is associated with several types of DNA polymerases, such as PolIIIA and family X DNA polymerases, stand alone histidinol phosphate phosphatases (HisPPases), and a number of uncharacterized protein families. HisPPase catalyzes the eighth step of histidine biosynthesis, in which L-histidinol phosphate undergoes dephosphorylation to produce histidinol. The PHP domain has four conserved sequence motifs and contains an invariant histidine that is involved in metal ion coordination. The PHP domain of HisPPase is structurally homologous to other members of the PHP family that have a distorted (beta/alpha)7 barrel fold with a trinuclear metal site on the C-terminal side of the barrel.	4.77991e-82
NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein	gnl|CDD|275083	TIGR04256, conserved_hypothetical_protein, GxxExxY protein. Members of this protein family average about 130 residues in length and include an almost perfectly conserved motif GxxExxY. Members occur in a wide range of prokaryotes, including Proteobacteria, Perrucomicrobia, Cyanobacteria, Bacteriodetes, Archaea, etc.	1.64615e-11
NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein	gnl|CDD|240532	cd13127, MATE_tuaB_like, Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins. This family might function as a translocase for lipopolysaccharides and participate in the biosynthesis of cell wall components such as teichuronic acid. The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. A number of family members are involved in the synthesis of peptidoglycan components in bacteria.	3.52653e-95
NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein	gnl|CDD|226288	COG3765, WzzB, Chain length determinant protein [Cell envelope biogenesis, outer membrane].	1.41483e-10

Protein sequences

>NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase
MYPFTHYFYIIASFLFSLLVCYRVIPSVIKLAREKKLYDEPNHRSSHKKVVPSLGGVAIFLGFTLSVLIFSNGYMIHELKYIIAATLIMFGLGIKDDLMDISAKKKLAGQIFAALVVIVMGGIRFTNFHGIFGIHELNYFTSVVFTIFVIIVVTNGFNLIDGIDGLASGISGLCTFSFGLWFYLTGNYQYALLSASLLGALISFFKFNVFGKDLKIFMGDTGSMILGLLISILTIQFNELNINPDFKYAIVAAPAVSIGILAIPLFDTIRVMLIRIKNKKSPFQPDKNHVHHRMVSLGYKHAAATFRIMAVNILFIVTAFLLDWTGNAILLSLVICMGSFFSWFPSYLLNRKKAKEAALQIKKEVLQTKKEPAVQTKRESEMKTKKESAFQTT
>NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein
MIGSIKKMFLTCLFALLVLGAANSQNTNINPASVNVEAMSDAQIEKLIAEMEKNGMSMEGAMALARARGASQLQIDQMVKKISEYKSGKRNGVTKDDELEEILVPEEKLSEKVAFKATDKNKEIFGFQFFNSKNLSFEPTMDVPVSESYALGIGDEVNIAVYGASQQNYQLTVQKNGAISIPDLGPVHVYGISFKDAQQKIKNRLISIYNGMGDDRPNTFSDVSMGNLKGVKVNVIGEVNLPGTYSLPATASAFNALYLAGGPNENGSFRSIDVLRDGKLVCSVDVYAYLIDGKTQNNIQLRDQDVVLVRPYAKRVRVEGEFKRTGLFEAQSDETVADLLRFAGGFTDKAYTHRLELYRNNTRTLTFKGVSSDQYDQVQLMNGDRLVAGMLTERFENRVSIDGAVYRPGNYELTEGLQLSELIQKAEGLKEEAFMERAVITRKQEDLSLKAVSFSVRDVVNGKIDFELQREDRVQISSIFDMREARTVEIVGEVQFAGTFAWAENLRIGDLIFQAGGFKEEAEVAGIEVSRVLDYDETSELTKSLLHTFQFSLDRNLKLNPQDDSFLLQPFDKVYVRRAPGYRPQGVANIQGEVKYAGDFGITRKDEKISDIIRRAGGITPEAYVRGASLRRRIEMSEAEYQAKLAIVAQDTTMMGQEIEKVSYQIVGIDLATILANPGAVQDLQLQGGDQILVPSKLETVMVSGSVLSPVAHTFTQQKKLKDYVNGSGGFSERAKKGKVYVLYANGTTQATKGGLFGRRFPKVEPGCEIIVPEKPEVDKAASASKWLAIASTLAALITAMAVATR
>NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein
MVDLLHKDECYAIVGACMNVHSQLGSGFLESVYAEALGKEFKKRNIPFVKEKKLELYYDGEKMNKYFKADFICFGSIIVELKSKTILLKIDEQQTINYLKATNYQLGLLINFGERSLKYKRFVNTIEKN
>NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein
MKEIQESKHTETTSANREDEIDLIQLAKTLWNGRRTVIRTTLIFMVLGLFIAIFSAKEYTATTTMVPQSAEGGSKLGGLGGLAAMAGINLGSMGGGSSIPPTLYPKIIKSISFQKELMQTLLSIEGQSGEVTFADYYLEIKKPGLLGYIKKYSIGLPGLIIGATIKAIKGNSTDATLASSSDEILSISMEEKQLIEILTAQLSLEVNDKDGYVSLSACMPEAKAAAQLAKKAQKLLQQAIIDFKIQKAKDQLDFVEERFAEKEAVFNAVQVKLARFRDQNKNVSSAVAQTQLERLQSDFSMASSVYTELAKQLETQKIQVKEDTPVFTIIEPVSIPLEKSKPRRTIILVIWTFLGGIVGVGMVFGKEFFVSIKDKWKEEGVNSTNES
>NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein
MLKIDEQQTINYLKASNYELDLLVNFGERSLKYKRFVNTKH
>NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase
MTKVKTITNICCIGAGYVGGPTMAVIAQKCPHIKVTVVDVNEQRIADWNDADLNKLPIYEPGLADVVAEARGRNLFFSTDVDQAIDEAQMIFISVNTPTKTYGVGKGMAADLKYIELCARQIAKVSKDDKIIVEKSTLPVRTAEALSTILNSTGNGANFDILSSPEFLAEGTAISDLQKPDRILIGGDETEKGQSAIKSLVAVYENWVPRENIITTRVWSSELSKLTANAFLAQRVSSINAISALCEKTGADVDEISRAIGADSRIGSKFLKSSVGFGGSCFQKDILNLVYLCRHFNLPEVADYWEQVIKMNDYQKHRFAKNIIDTLFNTVSGKKIAFLGWAFKKDTNDTRESAAIYVAKELLEDRAEIHIYDPKVSKEQIFADLKSVCHPEGSEATKDLFKNVIVHKDPYTAMQSAHAIAILTEWDEFKTYDWNKVYDEMLKPAFLFDGRNVVSNEELEKIGFQVYNIGKG
>NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein
MNLKKKSIDGAKWTTFSTVVVALSAIIKASILTRIIEVSDFGLMALVTFILGFIIIFIDLGLTTAILHRQKITKNEYSSLFWLNLGFSIFLFFILFLVTSFISEFYKESELMTLIPLASIGLIVNAMGCQFRTVEQKELNFKLLALVDIFSSVVSLGVAVILAINDFGVYSLVYSSLAYTTLVSFFYFIKGLIAKRLKFHFMFSDAKPFLKIGVFNIGGQVINYFIKEVDILIVGNVFGTEVLGGYSLAKQLVDRPLRVIRPVVLKVMAPILSLIQGDKISLRENYLKIIKFVSSISIPIYLLFILFAKPIVLLFYGAGYQEIEILVRILSIYMFFISIKIPSGSLIIATGKTNLEFYWFLASFIIMPIGILIGSKFSIEAVASAMVIVMVIYFIPMWKFLINPLIKVEFSDYYKAHIPSLKLLKQVFIKKND
>NZ_AP018042.1|WP_096427574.1|326858_327959_-|hypothetical-protein
MCIIEEVDINNKLNNEIQIIVNESIKLVSDSLLAIVLYGSYGRGEGAFFSDEEGKIRTYNDFDIILVVSDLLEQEIINEISNTLSDKLDVKWIDISQKSKKTLKNLKLSIFNYDFKYGSRVIYGDNLILEDIPKFQNIHLSLKEAETLYFTRIWTFLGSLPKNGFESGVSGEDSRFFKNQMAKAILAIVDIVLLQKKCYDTSYRKRVELINNTCQNKPDLIKWSNWAIKEKCRPSGNKLSPLDVETMYSEIANIFLVEMFHVLSIYYKKKINCSKDIESSLRFSFSEFVVTTKLFLKNKRLKYIKNKKIGLAQSYLLEAYIKEGKQKVDLINKSKKIVKSIDSSIDLRKLDWNELRLLVSELRMYL
>NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein
MKVVFFLLDAFKEEYLSEDITPFLFEKAKEGKHIKKIIPSAGFCERTEIFFGLRPKESGFFTAIGFDYEKGLYKNLWTLNFFGWIENVISYLAIVLLKRKKNIIDKYCHSILLKIYLKFFGTSKKLKPYKIPLSFLSYFNLTEDEFDLYKRDEINSKKSLFKVVEEFKGRTYIDAFTSLGGISNGDDENRIKLAIEASKNVENVFIPIYISISDSAGHYHGPNSERLKSELLKLDSLLQSSVEKFSEIDSSTKFLFLGDHGMTEVVKDIDVKMKISEIAIKHRLKTGTDFIYFLDSTLLRMWFFNDKSRNIFSEEIKNDSSFNENGIVINADVSSKYYLPVGDRKYGDLTWWANEGVLIFPDFFHNDKALKGMHGYRPDTQSTYGTCIVWDEHKVNDEVEKLELFQVYDLINNLLKD
>NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase
MKKIYIAGSGGMLGEGFYKIFNNEYKLKCSDKDVNEDWLTFLDFRDFNAYRKDVLDFNPDYLFHLGAYTDLEYCEEHSDDTYMTNTLAVENAVRIANELNIPLLYISTAGIFDGKKDFYDDWDMPNPLGVYARSKYMGERYVCENANRYLVCRAGWMMGSGPKKDKKFVQKLMMQIKDGKQELFIVNDKDGTPTYTHDFANSVKELIQKEYWGLYNMVCGGQTSRFEVAKTLVKYLQLPYDIKITPVSSDYFKEEYYAQRPASERLVAKKLDLRKVNKMRDWKISLKEYLDSYYQGFLDNK
>NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein
MENNLQKRLFQFSIDIIKEVRNLPNSKEYQVISYQILKSATSVGANYEEAQGAVSKADFANKVGISLKEIRETNYWIRIIIAIDNQKINWAVLEKESNELIRILGTIYNKTSKNKKVVSSRIPDTIS
>NZ_AP018042.1|WP_096427582.1|340858_342046_-|SPOR-domain-containing-protein
MRNIYFLLCFFLTVGTIGFFSPFFDINLTSSTAGGSTYGVEIAKFKYPVYTDYFEEMDDVVELSGKDGEYHYVAGLTNSISSAEKLAEEIKSYGYADAKVLDLNSEFSKEQIARVLDNESIDQDQTKKQNITTQTVPKNKAAEIAIGKLTNIGNAYFYTILLQKSKDILNAESFSPHNSVKILEANGKFQYVLGRFEDVGDAKKYLKEKIIVDFPNAVVAVVNKGKLAEVREVQKVNTKMEIASNQTGSYNMGRKMRGKEYVDYYYELGRLKISEKPLYVIEIGEFNDKALADEAVQKLKDLGFTQARVKTPSSSKVGAVTESLSVNAHFTIQVFASKAEMSVKRLTIKNLTRRFDQEDELYRYFYGDYDNYWVCRRELREIREKGYGDAFIVKL
>NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein
MIHKLKLLVLFLLLYNVLFAQSKFEKKGDKAFLKKEFAEAIIHFKNSKEKNTVIIRKIAECYFILGNYDESEKFFNSIKEEEKISTDLLHLSQIYLAKDNFAAAVLFIERAGELGAEPAEIQNRVGVVRELIEFRNENQDSKIIPIGVQPKGKCLGIFLQPEGIIYSDTGSSTIKSDKNYQLFIASGEGLEFASSKSFAGTLEPKTDLGAVCISPDGNTMYYTRWYVRKGKQQMEIAIAKKQKGEWISKKSLAFCNRKYSCCYPFLSPDGKAMYFSSDMEGGYGGMDLYVSQQEGRKWTEPKNLGVSINSAQNEIYPRMLSNNQLWFSSDGRAGYGQLDLFFTSKNENGAWAQVVNPGAPYNSQYSDYSILDIPDGNIQLFVSDREDKGLRDRIYQLKKETTESVQILVQDKQSKQFIKDVNISVRKAFENKEITLQNKENSEGEISFKIPKSEFDKGILYEIAVKKSGYIDQIIEYYPSNKKMNVEVNLDRIVETNQFNFVSELMPIAYPDKKITFQNIYFEKNVDDFSEDAKKILDRLYRFWKEFPELGIKINGHSDSKGMEQMNLDISLQKAAEAKKYLVGKGMKPSVIELGAWGEEFILNGCFDDSECSEKEHQENRRLELIFVL
>NZ_AP018042.1|WP_145957556.1|345027_345306_+|hypothetical-protein
MRIAFYKYIPSCADPVSHHLITLDIGLLTSVPLDFLDLRLSTIGLLDLSTLKPLYFPAQMRIAFYKYIPSCADPVSHHLIPGWHSLRSFTLG
>NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase
MDAKQIQIADFTYDLPDHRIAKHPLENRDLSKLLIYKKGEISESVFENLPSEIADGTTMVFNNTKVIQARLIFHKETGARIEIFCLEPLKPADYNLAFQACGSSTWKCIVGNARKWKFGVLKMPFYLDEEEYCLTAEQQEVLDGAQEILFTWNHPSLSFSDVLEQTGKIPIPPYLNRDTEATDLVRYQTVYSKYEGSVAAPTAGLHFTEDVFSRLKAKNINREELTLHVGAGTFKPVKSELIGDHEMHTEHIQVTRESLESISKTTGQIVAVGTTSVRSLESLYWMGVKILTQQENPHLLKQWEAYQLPINYSVKESYQAICSHMEKNDLTLFNAATQIIIAPGYDFKVISGLVTNFHQPQSTLLLLISALVGDNWKKIYRYALENNFRFLSYGDSSLLMKE
>NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein
MQINSGDKVRFLNETGGGIVTRIIDAKTVMVLNDEDEFEIPSLKRNLVVIESASTGNSPQLKQSNSTSASFTSTKQSLFVEKEEVYAAFAPKESSTPSESPLELYLINDTNQIILYNFFDEKGNGLEGVTAGTLDPKSKILLAEYEIKELGELTCCQFQIIFYQQGKCTPKSPLNRTLKIQAVKFHKSSSYKDTAYFHQEVILHKLTGEIMEQKLEELSDKDFKQVIREKGKSEKAKPALKKNNSDNNIVEVDLHVNALIDSVTGLSNADILELQMNKFHEVLRQHQNDKGKKIVFIHGIGNGTLKQHVHSELKRKYKKHYEQDASFKEYGWGATMVTIR
>NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter
MVSIPLGPIGVLIIQKTIQKGRLAGFVSGMGAAVADMFYATVAAFGLGMVLSFIETQEFYLQLVGSVFLVYVGLRIFFTNPIKQIRGAKKTGKKGMLGDFVSIFFLTASNPIAVFVFVAVFAGTSIFGTNPTLRIELFLILGVLLGGGLWWYTLSTIINIFRKKFRLKQLFWINKISGIVIAVLGFLAFLACFEPIKSFLHS
>NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein
MKLVDAKDLLKASENMNWLGGESFAKLLMYVLRLNKLNDLYSANCGKEGLEFIDALLEDLGIKFEFDEEELKRIPKEGPFMTISNHPFGGIDGLILIKLVCMVRPEFRVMANFLLKKIEPIKDYFLGVNPFEERKGVASSTGGIKEGLRHLSDGFPLGIFPAGEVSSYQSHTNNITDKQWQPSILKFIKKAEVPVVPIYFQGSNSLIFHLLGMIHPALRTVKLPSELLNKKNKIVRIRIGNPIPVKDQEGFTDINQYGRFLRAKTYMLGTPIEVKKFYQFKRKEKIAKVEDIIPPVNPEIIQREVEGLAQEYLLFKTNNFVVYCAPSAQMPNVLTEIGRLREITFREVGEGTNLGIDIDEYDLYYHQLFIWDEELKKIVGAYRVGKGNEILSEYGLKGFYLQSLFRISKKFQPILHESLELGRSFIVKEYQKKPMPLFLLWKGILYFLLKNPQSRYLIGPVSISNKYSNLSKDLIIKFIMRNYFNYDLGQYIKSRKKFKVKIKGLDVDILLEMAKNDINKLDRLIGDFEITNDKLPVLLKKYISLNAKIVGFNIDPNFNDCLDGLIVVDLFNVPIKVIESLSKEFNDDSIMKRFSSEDVRF
>NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ
MNYFSYHTHTQYCDGKSTAEELVLRAIELGLTAIGFSSHAPLPKKESWSMSMDMLEDYIAEVQELKQKYKSQIEVYVSLEIDYIPGVTRSFDEFKRESNLDYTIGSVHLVKAKYESEFWFLDGPDTNYIHGIEVLFDGDVKKAVSAYYNQVIDLIKTQKPDVIAHVDKVKMNNKGRYFSEDEKWYVALLDKTIEVIKTSGTIVEVNTRGIYKKKSGSFFPDSYFLKECEKRAIPVTVSCDAHHADELVSGFDSAIGLLKGIGFESVRVFADGNWRDQSL
>NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein
MTENNRLHIDKWLPTSAKEVKERGWEELDVILFSGDAYIDHPSFGASVIGRVLEKEGLKVAIVPQPNWRDDLRDFKKLGVPKLFFAVTAGNMDSMVNHYTANKRLRHDDAYTPEGRHGQRPDRPTIVYSQILKKLYPDTPIVIGGIEASLRRVTHYDYWDDELKPSILCESDADLLVYGMGEKPLKEIVRLTRKGVPFYSLTNIPQTSFLVSKDESYPTKKQWQDQELYSHEDCLNDKKKFASNFRYIEMESNAMMAKKLTQDYKDQSIIVNPPYPVMNEKEMDEVYDLPYTRLPHPRYKDKKIPAFDMIKFSVNMHRGCFGGCSFCTISAHQGKFIANRSEKSILKEVENITKMPDFKGYLSDLGGPSANMYQMKGIFESICKTCRRPSCIHPDVCPNLNTNHQAMLDIYKKVDQVPGVKKSFVGSGIRYDLILHQTKDAKINKSNREYATELIKNHVSGRLKVAPEHTSDEVLNVMRKPSFNLFYKLKALFDDINKKEGLNQQLIPYFISSHPGSHGTDMADLAVKTKELDFKLEQVQDFTPTPMTVATVIYYSGYHPYTLKKVFTAKSVKEKLSQRKFLFWYKKEYREAIKTELIKMGKKDMLTKLFK

Crispr_ID: NZ_AP018042_1

• CRISPR_ID: NZ_AP018042_1
• CRISPR_location: 338251-338321
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_1

Consensus_repeat	Method
TTTGGACTTTGGACTTTTGGACTTT	CRISPRCasFinder

spacers of NZ_AP018042_1

>1.1|338276|20|NZ_AP018042|CRISPRCasFinder
GGACTTTTGGACTTTGGACT

CRISPR arrays and Neighbor proteins around NZ_AP018042_1

CRISPR arrays

The CRISPR arrays of NZ_AP018042_1

>merge|NZ_AP018042|1|338251-338321|CRISPRCasFinder
TCAGGACTTTGGACTTTTGGACTTTGGACTTTTGGACTTTGGACTTTTGGACTTTGGACTTTTGGACTTTT

>NZ_AP018042|1|1|338251-338321|CRISPRCasFinder
TCAGGACTTTGGACTTTTGGACTTT	GGACTTTTGGACTTTGGACT
TTTGGACTTTGGACTTTTGGACTTTT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096427582.1|340858_342046_-|SPOR-domain-containing-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase	unknown	unknown	gnl|CDD|133463
NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase	unknown	unknown	gnl|CDD|187564
NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase	unknown	unknown	gnl|CDD|177986
NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein	unknown	unknown	gnl|CDD|274132
NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein	unknown	unknown	gnl|CDD|370595
NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein	unknown	unknown	gnl|CDD|275083
NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase	unknown	unknown	gnl|CDD|234666
NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein	unknown	unknown	gnl|CDD|293742
NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein	unknown	unknown	gnl|CDD|143586
NZ_AP018042.1|WP_145957556.1|345027_345306_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein	unknown	unknown	gnl|CDD|153248
NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein	unknown	unknown	gnl|CDD|132073
NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter	unknown	unknown	gnl|CDD|273358
NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein	unknown	unknown	gnl|CDD|234874
NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ	unknown	unknown	gnl|CDD|213994
NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein	unknown	unknown	gnl|CDD|275083
NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein	unknown	unknown	gnl|CDD|240532
NZ_AP018042.1|WP_096427574.1|326858_327959_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein	unknown	unknown	gnl|CDD|226288

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase	gnl|CDD|133463	cd06853, GT_WecA_like, This subfamily contains Escherichia coli WecA, Bacillus subtilis TagO and related proteins. WecA is an UDP-N-acetylglucosamine (GlcNAc):undecaprenyl-phosphate (Und-P) GlcNAc-1-phosphate transferase that catalyzes the formation of a phosphodiester bond between a membrane-associated undecaprenyl-phosphate molecule and N-acetylglucosamine 1-phosphate, which is usually donated by a soluble UDP-N-acetylglucosamine precursor. WecA participates in the biosynthesis of O antigen LPS in many enteric bacteria and is also involved in the biosynthesis of enterobacterial common antigen. A conserved short sequence motif and a conserved arginine at a cytosolic loop of this integral membrane protein were shown to be critical in recognition of substrate UDP-N-acetylglucosamine.	5.93933e-85
NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase	gnl|CDD|187564	cd05254, dTDP_HR_like_SDR_e, dTDP-6-deoxy-L-lyxo-4-hexulose reductase and related proteins, extended (e) SDRs. dTDP-6-deoxy-L-lyxo-4-hexulose reductase, an extended SDR, synthesizes dTDP-L-rhamnose from alpha-D-glucose-1-phosphate, providing the precursor of L-rhamnose, an essential cell wall component of many pathogenic bacteria. This subgroup has the characteristic active site tetrad and NADP-binding motif. This subgroup also contains human MAT2B, the regulatory subunit of methionine adenosyltransferase (MAT); MAT catalyzes S-adenosylmethionine synthesis. The human gene encoding MAT2B encodes two major splicing variants which are induced in human cell liver cancer and regulate HuR, an mRNA-binding protein which stabilizes the mRNA of several cyclins, to affect cell proliferation. Both MAT2B variants include this extended SDR domain. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	9.05753e-60
NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase	gnl|CDD|177986	PLN02353, PLN02353, probable UDP-glucose 6-dehydrogenase.	0
NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein	gnl|CDD|293742	cd16018, Enpp, Ectonucleotide pyrophosphatase/phosphodiesterase, also called autotaxin. Ecto-nucleotide pyrophosphatases/phosphodiesterases (ENPPs) hydrolyze 5'-phosphodiester bonds in nucleotides and their derivatives, resulting in the release of 5'-nucleotide monophosphates. ENPPs have multiple physiological roles, including nucleotide recycling, modulation of purinergic receptor signaling, regulation of extracellular pyrophosphate levels, stimulation of cell motility, and possible roles in regulation of insulin receptor (IR) signaling and activity of ecto-kinases. The eukaryotic ENPP family contains at least five members that have different tissue distribution and physiological roles.	2.34552e-08
NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein	gnl|CDD|370595	pfam09640, DUF2027, Domain of unknown function (DUF2027). This protein domain is of unknown function. though putatively involved in DNA mismatch repair. It is associated with pfam01713.	3.59492e-28
NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein	gnl|CDD|275083	TIGR04256, conserved_hypothetical_protein, GxxExxY protein. Members of this protein family average about 130 residues in length and include an almost perfectly conserved motif GxxExxY. Members occur in a wide range of prokaryotes, including Proteobacteria, Perrucomicrobia, Cyanobacteria, Bacteriodetes, Archaea, etc.	4.86973e-53
NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase	gnl|CDD|234666	PRK00147, queA, S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Provisional.	2.54275e-133
NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein	gnl|CDD|274132	TIGR02436, S23_ribosomal_protein, four helix bundle protein. This family describes a protein of unknown function whose structure is a bundle of four long alpha helices. Some of the first members of this family were found encoded in the (atypically large) intervening sequence (IVS) of Leptospira 23S RNA, a region often present in the rRNA gene and removed during rRNA processing without re-ligation. However, this location is not conserved, and naming this protein as a 23S RNA protein is both confusing and inaccurate.	2.4894e-23
NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein	gnl|CDD|143586	cd07185, OmpA_C-like, Peptidoglycan binding domains similar to the C-terminal domain of outer-membrane protein OmpA. OmpA-like domains (named after the C-terminal domain of Escherichia coli OmpA protein) have been shown to non-covalently associate with peptidoglycan, a network of glycan chains composed of disaccharides, which are crosslinked via short peptide bridges. Well-studied members of this family include the Escherichia coli outer membrane protein OmpA, the Escherichia coli lipoprotein PAL, Neisseria meningitdis RmpM, which interact with the outer membrane, as well as the Escherichia coli motor protein MotB, and the Vibrio flagellar motor proteins PomB and MotY, which interact with the inner membrane.	1.73983e-18
NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein	gnl|CDD|153248	cd07986, LPLAT_ACT14924-like, Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: Unknown ACT14924. Lysophospholipid acyltransferase (LPLAT) superfamily member: acyltransferases of de novo and remodeling pathways of glycerophospholipid biosynthesis which catalyze the incorporation of an acyl group from either acylCoAs or acyl-acyl carrier proteins (acylACPs) into acceptors such as glycerol 3-phosphate, dihydroxyacetone phosphate or lyso-phosphatidic acid. Included in this subgroup are uncharacterized phospholipid/glycerol acyltransferases such as the Pectobacterium carotovorum subsp. carotovorum PC1 locus ACT14924 putative acyltransferase, and similar proteins.	3.91246e-82
NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein	gnl|CDD|132073	TIGR03028, EpsE, polysaccharide export protein EpsE. Sequences in this family of proteins are members of a polysaccharide export protein family (pfam02563) which includes the wza protein from E.coli. This family of proteins are homologous to the EpsE protein of the methanolan biosynthesis operon of Methylobacillus species strain 12S. The distribution of this protein appears to be restricted to a subset of exopolysaccharide operons containing a syntenic grouping of genes including a variant of the EpsH exosortase protein. Exosortase has been proposed to be involved in the targetting and processing of proteins containing the PEP-CTERM domain to the exopolysaccharide layer.	1.08433e-20
NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter	gnl|CDD|273358	TIGR00949, Uncharacterized_membrane_protein_YahN, The Resistance to Homoserine/Threonine (RhtB) Family protein. [Transport and binding proteins, Amino acids, peptides and amines].	3.75241e-11
NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein	gnl|CDD|234874	PRK00955, PRK00955, YgiQ family radical SAM protein.	0
NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ	gnl|CDD|213994	cd12110, PHP_HisPPase_Hisj_like, Polymerase and Histidinol Phosphatase domain of Histidinol phosphate phosphatase of Hisj like. Bacillus subtilis YtvP HisJ has strong histidinol phosphate phosphatase (HisPPase) activity. The PHP (also called histidinol phosphatase-2/HIS2) domain is associated with several types of DNA polymerases, such as PolIIIA and family X DNA polymerases, stand alone histidinol phosphate phosphatases (HisPPases), and a number of uncharacterized protein families. HisPPase catalyzes the eighth step of histidine biosynthesis, in which L-histidinol phosphate undergoes dephosphorylation to produce histidinol. The PHP domain has four conserved sequence motifs and contains an invariant histidine that is involved in metal ion coordination. The PHP domain of HisPPase is structurally homologous to other members of the PHP family that have a distorted (beta/alpha)7 barrel fold with a trinuclear metal site on the C-terminal side of the barrel.	4.77991e-82
NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein	gnl|CDD|275083	TIGR04256, conserved_hypothetical_protein, GxxExxY protein. Members of this protein family average about 130 residues in length and include an almost perfectly conserved motif GxxExxY. Members occur in a wide range of prokaryotes, including Proteobacteria, Perrucomicrobia, Cyanobacteria, Bacteriodetes, Archaea, etc.	1.64615e-11
NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein	gnl|CDD|240532	cd13127, MATE_tuaB_like, Uncharacterized subfamily of the multidrug and toxic compound extrusion (MATE) proteins. This family might function as a translocase for lipopolysaccharides and participate in the biosynthesis of cell wall components such as teichuronic acid. The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. A number of family members are involved in the synthesis of peptidoglycan components in bacteria.	3.52653e-95
NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein	gnl|CDD|226288	COG3765, WzzB, Chain length determinant protein [Cell envelope biogenesis, outer membrane].	1.41483e-10

Protein sequences

>NZ_AP018042.1|WP_096427579.1|336001_337183_+|undecaprenyl/decaprenyl-phosphate-alpha-N-acetylglucosaminyl-1-phosphate-transferase
MYPFTHYFYIIASFLFSLLVCYRVIPSVIKLAREKKLYDEPNHRSSHKKVVPSLGGVAIFLGFTLSVLIFSNGYMIHELKYIIAATLIMFGLGIKDDLMDISAKKKLAGQIFAALVVIVMGGIRFTNFHGIFGIHELNYFTSVVFTIFVIIVVTNGFNLIDGIDGLASGISGLCTFSFGLWFYLTGNYQYALLSASLLGALISFFKFNVFGKDLKIFMGDTGSMILGLLISILTIQFNELNINPDFKYAIVAAPAVSIGILAIPLFDTIRVMLIRIKNKKSPFQPDKNHVHHRMVSLGYKHAAATFRIMAVNILFIVTAFLLDWTGNAILLSLVICMGSFFSWFPSYLLNRKKAKEAALQIKKEVLQTKKEPAVQTKRESEMKTKKESAFQTT
>NZ_AP018042.1|WP_096427578.1|332924_335345_-|SLBB-domain-containing-protein
MIGSIKKMFLTCLFALLVLGAANSQNTNINPASVNVEAMSDAQIEKLIAEMEKNGMSMEGAMALARARGASQLQIDQMVKKISEYKSGKRNGVTKDDELEEILVPEEKLSEKVAFKATDKNKEIFGFQFFNSKNLSFEPTMDVPVSESYALGIGDEVNIAVYGASQQNYQLTVQKNGAISIPDLGPVHVYGISFKDAQQKIKNRLISIYNGMGDDRPNTFSDVSMGNLKGVKVNVIGEVNLPGTYSLPATASAFNALYLAGGPNENGSFRSIDVLRDGKLVCSVDVYAYLIDGKTQNNIQLRDQDVVLVRPYAKRVRVEGEFKRTGLFEAQSDETVADLLRFAGGFTDKAYTHRLELYRNNTRTLTFKGVSSDQYDQVQLMNGDRLVAGMLTERFENRVSIDGAVYRPGNYELTEGLQLSELIQKAEGLKEEAFMERAVITRKQEDLSLKAVSFSVRDVVNGKIDFELQREDRVQISSIFDMREARTVEIVGEVQFAGTFAWAENLRIGDLIFQAGGFKEEAEVAGIEVSRVLDYDETSELTKSLLHTFQFSLDRNLKLNPQDDSFLLQPFDKVYVRRAPGYRPQGVANIQGEVKYAGDFGITRKDEKISDIIRRAGGITPEAYVRGASLRRRIEMSEAEYQAKLAIVAQDTTMMGQEIEKVSYQIVGIDLATILANPGAVQDLQLQGGDQILVPSKLETVMVSGSVLSPVAHTFTQQKKLKDYVNGSGGFSERAKKGKVYVLYANGTTQATKGGLFGRRFPKVEPGCEIIVPEKPEVDKAASASKWLAIASTLAALITAMAVATR
>NZ_AP018042.1|WP_173803983.1|332444_332834_-|GxxExxY-protein
MVDLLHKDECYAIVGACMNVHSQLGSGFLESVYAEALGKEFKKRNIPFVKEKKLELYYDGEKMNKYFKADFICFGSIIVELKSKTILLKIDEQQTINYLKATNYQLGLLINFGERSLKYKRFVNTIEKN
>NZ_AP018042.1|WP_096427577.1|331206_332370_-|exopolysaccharide-biosynthesis-protein
MKEIQESKHTETTSANREDEIDLIQLAKTLWNGRRTVIRTTLIFMVLGLFIAIFSAKEYTATTTMVPQSAEGGSKLGGLGGLAAMAGINLGSMGGGSSIPPTLYPKIIKSISFQKELMQTLLSIEGQSGEVTFADYYLEIKKPGLLGYIKKYSIGLPGLIIGATIKAIKGNSTDATLASSSDEILSISMEEKQLIEILTAQLSLEVNDKDGYVSLSACMPEAKAAAQLAKKAQKLLQQAIIDFKIQKAKDQLDFVEERFAEKEAVFNAVQVKLARFRDQNKNVSSAVAQTQLERLQSDFSMASSVYTELAKQLETQKIQVKEDTPVFTIIEPVSIPLEKSKPRRTIILVIWTFLGGIVGVGMVFGKEFFVSIKDKWKEEGVNSTNES
>NZ_AP018042.1|WP_145957555.1|331007_331133_-|hypothetical-protein
MLKIDEQQTINYLKASNYELDLLVNFGERSLKYKRFVNTKH
>NZ_AP018042.1|WP_096427576.1|329482_330901_-|UDP-glucose-6-dehydrogenase
MTKVKTITNICCIGAGYVGGPTMAVIAQKCPHIKVTVVDVNEQRIADWNDADLNKLPIYEPGLADVVAEARGRNLFFSTDVDQAIDEAQMIFISVNTPTKTYGVGKGMAADLKYIELCARQIAKVSKDDKIIVEKSTLPVRTAEALSTILNSTGNGANFDILSSPEFLAEGTAISDLQKPDRILIGGDETEKGQSAIKSLVAVYENWVPRENIITTRVWSSELSKLTANAFLAQRVSSINAISALCEKTGADVDEISRAIGADSRIGSKFLKSSVGFGGSCFQKDILNLVYLCRHFNLPEVADYWEQVIKMNDYQKHRFAKNIIDTLFNTVSGKKIAFLGWAFKKDTNDTRESAAIYVAKELLEDRAEIHIYDPKVSKEQIFADLKSVCHPEGSEATKDLFKNVIVHKDPYTAMQSAHAIAILTEWDEFKTYDWNKVYDEMLKPAFLFDGRNVVSNEELEKIGFQVYNIGKG
>NZ_AP018042.1|WP_096427575.1|327969_329271_-|MOP-flippase-family-protein
MNLKKKSIDGAKWTTFSTVVVALSAIIKASILTRIIEVSDFGLMALVTFILGFIIIFIDLGLTTAILHRQKITKNEYSSLFWLNLGFSIFLFFILFLVTSFISEFYKESELMTLIPLASIGLIVNAMGCQFRTVEQKELNFKLLALVDIFSSVVSLGVAVILAINDFGVYSLVYSSLAYTTLVSFFYFIKGLIAKRLKFHFMFSDAKPFLKIGVFNIGGQVINYFIKEVDILIVGNVFGTEVLGGYSLAKQLVDRPLRVIRPVVLKVMAPILSLIQGDKISLRENYLKIIKFVSSISIPIYLLFILFAKPIVLLFYGAGYQEIEILVRILSIYMFFISIKIPSGSLIIATGKTNLEFYWFLASFIIMPIGILIGSKFSIEAVASAMVIVMVIYFIPMWKFLINPLIKVEFSDYYKAHIPSLKLLKQVFIKKND
>NZ_AP018042.1|WP_096427574.1|326858_327959_-|hypothetical-protein
MCIIEEVDINNKLNNEIQIIVNESIKLVSDSLLAIVLYGSYGRGEGAFFSDEEGKIRTYNDFDIILVVSDLLEQEIINEISNTLSDKLDVKWIDISQKSKKTLKNLKLSIFNYDFKYGSRVIYGDNLILEDIPKFQNIHLSLKEAETLYFTRIWTFLGSLPKNGFESGVSGEDSRFFKNQMAKAILAIVDIVLLQKKCYDTSYRKRVELINNTCQNKPDLIKWSNWAIKEKCRPSGNKLSPLDVETMYSEIANIFLVEMFHVLSIYYKKKINCSKDIESSLRFSFSEFVVTTKLFLKNKRLKYIKNKKIGLAQSYLLEAYIKEGKQKVDLINKSKKIVKSIDSSIDLRKLDWNELRLLVSELRMYL
>NZ_AP018042.1|WP_096427573.1|325608_326862_-|alkaline-phosphatase-family-protein
MKVVFFLLDAFKEEYLSEDITPFLFEKAKEGKHIKKIIPSAGFCERTEIFFGLRPKESGFFTAIGFDYEKGLYKNLWTLNFFGWIENVISYLAIVLLKRKKNIIDKYCHSILLKIYLKFFGTSKKLKPYKIPLSFLSYFNLTEDEFDLYKRDEINSKKSLFKVVEEFKGRTYIDAFTSLGGISNGDDENRIKLAIEASKNVENVFIPIYISISDSAGHYHGPNSERLKSELLKLDSLLQSSVEKFSEIDSSTKFLFLGDHGMTEVVKDIDVKMKISEIAIKHRLKTGTDFIYFLDSTLLRMWFFNDKSRNIFSEEIKNDSSFNENGIVINADVSSKYYLPVGDRKYGDLTWWANEGVLIFPDFFHNDKALKGMHGYRPDTQSTYGTCIVWDEHKVNDEVEKLELFQVYDLINNLLKD
>NZ_AP018042.1|WP_096427572.1|324608_325514_-|SDR-family-oxidoreductase
MKKIYIAGSGGMLGEGFYKIFNNEYKLKCSDKDVNEDWLTFLDFRDFNAYRKDVLDFNPDYLFHLGAYTDLEYCEEHSDDTYMTNTLAVENAVRIANELNIPLLYISTAGIFDGKKDFYDDWDMPNPLGVYARSKYMGERYVCENANRYLVCRAGWMMGSGPKKDKKFVQKLMMQIKDGKQELFIVNDKDGTPTYTHDFANSVKELIQKEYWGLYNMVCGGQTSRFEVAKTLVKYLQLPYDIKITPVSSDYFKEEYYAQRPASERLVAKKLDLRKVNKMRDWKISLKEYLDSYYQGFLDNK
>NZ_AP018042.1|WP_096427581.1|339686_340070_-|four-helix-bundle-protein
MENNLQKRLFQFSIDIIKEVRNLPNSKEYQVISYQILKSATSVGANYEEAQGAVSKADFANKVGISLKEIRETNYWIRIIIAIDNQKINWAVLEKESNELIRILGTIYNKTSKNKKVVSSRIPDTIS
>NZ_AP018042.1|WP_096427582.1|340858_342046_-|SPOR-domain-containing-protein
MRNIYFLLCFFLTVGTIGFFSPFFDINLTSSTAGGSTYGVEIAKFKYPVYTDYFEEMDDVVELSGKDGEYHYVAGLTNSISSAEKLAEEIKSYGYADAKVLDLNSEFSKEQIARVLDNESIDQDQTKKQNITTQTVPKNKAAEIAIGKLTNIGNAYFYTILLQKSKDILNAESFSPHNSVKILEANGKFQYVLGRFEDVGDAKKYLKEKIIVDFPNAVVAVVNKGKLAEVREVQKVNTKMEIASNQTGSYNMGRKMRGKEYVDYYYELGRLKISEKPLYVIEIGEFNDKALADEAVQKLKDLGFTQARVKTPSSSKVGAVTESLSVNAHFTIQVFASKAEMSVKRLTIKNLTRRFDQEDELYRYFYGDYDNYWVCRRELREIREKGYGDAFIVKL
>NZ_AP018042.1|WP_096427583.1|342185_344075_-|OmpA-family-protein
MIHKLKLLVLFLLLYNVLFAQSKFEKKGDKAFLKKEFAEAIIHFKNSKEKNTVIIRKIAECYFILGNYDESEKFFNSIKEEEKISTDLLHLSQIYLAKDNFAAAVLFIERAGELGAEPAEIQNRVGVVRELIEFRNENQDSKIIPIGVQPKGKCLGIFLQPEGIIYSDTGSSTIKSDKNYQLFIASGEGLEFASSKSFAGTLEPKTDLGAVCISPDGNTMYYTRWYVRKGKQQMEIAIAKKQKGEWISKKSLAFCNRKYSCCYPFLSPDGKAMYFSSDMEGGYGGMDLYVSQQEGRKWTEPKNLGVSINSAQNEIYPRMLSNNQLWFSSDGRAGYGQLDLFFTSKNENGAWAQVVNPGAPYNSQYSDYSILDIPDGNIQLFVSDREDKGLRDRIYQLKKETTESVQILVQDKQSKQFIKDVNISVRKAFENKEITLQNKENSEGEISFKIPKSEFDKGILYEIAVKKSGYIDQIIEYYPSNKKMNVEVNLDRIVETNQFNFVSELMPIAYPDKKITFQNIYFEKNVDDFSEDAKKILDRLYRFWKEFPELGIKINGHSDSKGMEQMNLDISLQKAAEAKKYLVGKGMKPSVIELGAWGEEFILNGCFDDSECSEKEHQENRRLELIFVL
>NZ_AP018042.1|WP_145957556.1|345027_345306_+|hypothetical-protein
MRIAFYKYIPSCADPVSHHLITLDIGLLTSVPLDFLDLRLSTIGLLDLSTLKPLYFPAQMRIAFYKYIPSCADPVSHHLIPGWHSLRSFTLG
>NZ_AP018042.1|WP_096433316.1|346609_347818_-|S-adenosylmethionine:tRNA-ribosyltransferase-isomerase
MDAKQIQIADFTYDLPDHRIAKHPLENRDLSKLLIYKKGEISESVFENLPSEIADGTTMVFNNTKVIQARLIFHKETGARIEIFCLEPLKPADYNLAFQACGSSTWKCIVGNARKWKFGVLKMPFYLDEEEYCLTAEQQEVLDGAQEILFTWNHPSLSFSDVLEQTGKIPIPPYLNRDTEATDLVRYQTVYSKYEGSVAAPTAGLHFTEDVFSRLKAKNINREELTLHVGAGTFKPVKSELIGDHEMHTEHIQVTRESLESISKTTGQIVAVGTTSVRSLESLYWMGVKILTQQENPHLLKQWEAYQLPINYSVKESYQAICSHMEKNDLTLFNAATQIIIAPGYDFKVISGLVTNFHQPQSTLLLLISALVGDNWKKIYRYALENNFRFLSYGDSSLLMKE
>NZ_AP018042.1|WP_096427584.1|348007_349030_+|DUF2027-domain-containing-protein
MQINSGDKVRFLNETGGGIVTRIIDAKTVMVLNDEDEFEIPSLKRNLVVIESASTGNSPQLKQSNSTSASFTSTKQSLFVEKEEVYAAFAPKESSTPSESPLELYLINDTNQIILYNFFDEKGNGLEGVTAGTLDPKSKILLAEYEIKELGELTCCQFQIIFYQQGKCTPKSPLNRTLKIQAVKFHKSSSYKDTAYFHQEVILHKLTGEIMEQKLEELSDKDFKQVIREKGKSEKAKPALKKNNSDNNIVEVDLHVNALIDSVTGLSNADILELQMNKFHEVLRQHQNDKGKKIVFIHGIGNGTLKQHVHSELKRKYKKHYEQDASFKEYGWGATMVTIR
>NZ_AP018042.1|WP_096433318.1|349197_349806_-|LysE-family-transporter
MVSIPLGPIGVLIIQKTIQKGRLAGFVSGMGAAVADMFYATVAAFGLGMVLSFIETQEFYLQLVGSVFLVYVGLRIFFTNPIKQIRGAKKTGKKGMLGDFVSIFFLTASNPIAVFVFVAVFAGTSIFGTNPTLRIELFLILGVLLGGGLWWYTLSTIINIFRKKFRLKQLFWINKISGIVIAVLGFLAFLACFEPIKSFLHS
>NZ_AP018042.1|WP_096427585.1|349901_351707_-|lysophospholipid-acyltransferase-family-protein
MKLVDAKDLLKASENMNWLGGESFAKLLMYVLRLNKLNDLYSANCGKEGLEFIDALLEDLGIKFEFDEEELKRIPKEGPFMTISNHPFGGIDGLILIKLVCMVRPEFRVMANFLLKKIEPIKDYFLGVNPFEERKGVASSTGGIKEGLRHLSDGFPLGIFPAGEVSSYQSHTNNITDKQWQPSILKFIKKAEVPVVPIYFQGSNSLIFHLLGMIHPALRTVKLPSELLNKKNKIVRIRIGNPIPVKDQEGFTDINQYGRFLRAKTYMLGTPIEVKKFYQFKRKEKIAKVEDIIPPVNPEIIQREVEGLAQEYLLFKTNNFVVYCAPSAQMPNVLTEIGRLREITFREVGEGTNLGIDIDEYDLYYHQLFIWDEELKKIVGAYRVGKGNEILSEYGLKGFYLQSLFRISKKFQPILHESLELGRSFIVKEYQKKPMPLFLLWKGILYFLLKNPQSRYLIGPVSISNKYSNLSKDLIIKFIMRNYFNYDLGQYIKSRKKFKVKIKGLDVDILLEMAKNDINKLDRLIGDFEITNDKLPVLLKKYISLNAKIVGFNIDPNFNDCLDGLIVVDLFNVPIKVIESLSKEFNDDSIMKRFSSEDVRF
>NZ_AP018042.1|WP_162845361.1|351966_352806_-|histidinol-phosphatase-HisJ
MNYFSYHTHTQYCDGKSTAEELVLRAIELGLTAIGFSSHAPLPKKESWSMSMDMLEDYIAEVQELKQKYKSQIEVYVSLEIDYIPGVTRSFDEFKRESNLDYTIGSVHLVKAKYESEFWFLDGPDTNYIHGIEVLFDGDVKKAVSAYYNQVIDLIKTQKPDVIAHVDKVKMNNKGRYFSEDEKWYVALLDKTIEVIKTSGTIVEVNTRGIYKKKSGSFFPDSYFLKECEKRAIPVTVSCDAHHADELVSGFDSAIGLLKGIGFESVRVFADGNWRDQSL
>NZ_AP018042.1|WP_096427587.1|353006_354842_+|YgiQ-family-radical-SAM-protein
MTENNRLHIDKWLPTSAKEVKERGWEELDVILFSGDAYIDHPSFGASVIGRVLEKEGLKVAIVPQPNWRDDLRDFKKLGVPKLFFAVTAGNMDSMVNHYTANKRLRHDDAYTPEGRHGQRPDRPTIVYSQILKKLYPDTPIVIGGIEASLRRVTHYDYWDDELKPSILCESDADLLVYGMGEKPLKEIVRLTRKGVPFYSLTNIPQTSFLVSKDESYPTKKQWQDQELYSHEDCLNDKKKFASNFRYIEMESNAMMAKKLTQDYKDQSIIVNPPYPVMNEKEMDEVYDLPYTRLPHPRYKDKKIPAFDMIKFSVNMHRGCFGGCSFCTISAHQGKFIANRSEKSILKEVENITKMPDFKGYLSDLGGPSANMYQMKGIFESICKTCRRPSCIHPDVCPNLNTNHQAMLDIYKKVDQVPGVKKSFVGSGIRYDLILHQTKDAKINKSNREYATELIKNHVSGRLKVAPEHTSDEVLNVMRKPSFNLFYKLKALFDDINKKEGLNQQLIPYFISSHPGSHGTDMADLAVKTKELDFKLEQVQDFTPTPMTVATVIYYSGYHPYTLKKVFTAKSVKEKLSQRKFLFWYKKEYREAIKTELIKMGKKDMLTKLFK

Crispr_ID: NZ_AP018042_3

• CRISPR_ID: NZ_AP018042_3
• CRISPR_location: 676072-676159
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_3

Consensus_repeat	Method
GCGACTTTTATTTTATTAGCAAA	CRISPRCasFinder

spacers of NZ_AP018042_3

>3.1|676095|42|NZ_AP018042|CRISPRCasFinder
CCTTTCTTCAAATATCTCGGTGCTCGTCATCTTTAGGCACAT

CRISPR arrays and Neighbor proteins around NZ_AP018042_3

CRISPR arrays

The CRISPR arrays of NZ_AP018042_3

>merge|NZ_AP018042|3|676072-676159|CRISPRCasFinder
GCGACTTTTATTTTATTAGCAAACCTTTCTTCAAATATCTCGGTGCTCGTCATCTTTAGGCACATGCGACTTTTATTTTATTAGCAAA

>NZ_AP018042|3|3|676072-676159|CRISPRCasFinder
GCGACTTTTATTTTATTAGCAAA	CCTTTCTTCAAATATCTCGGTGCTCGTCATCTTTAGGCACAT
GCGACTTTTATTTTATTAGCAAA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096433346.1|676315_677338_-|L-threonine-3-dehydrogenase	unknown	unknown	gnl|CDD|180054
NZ_AP018042.1|WP_096427784.1|663540_664506_-|PstS-family-phosphate-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl|CDD|223304
NZ_AP018042.1|WP_188115969.1|680795_681206_-|hydrolase	unknown	unknown	gnl|CDD|223635
NZ_AP018042.1|WP_162845372.1|673300_674371_-|2Fe-2S-iron-sulfur-cluster-binding-domain-containing-protein	unknown	unknown	gnl|CDD|131215
NZ_AP018042.1|WP_096427792.1|677567_679010_-|site-specific-DNA-methyltransferase	unknown	unknown	gnl|CDD|225100
NZ_AP018042.1|WP_096427794.1|681322_683710_-|polysaccharide-biosynthesis-tyrosine-autokinase	unknown	unknown	gnl|CDD|349772
NZ_AP018042.1|WP_096427783.1|661531_662596_-|adenylate/guanylate-cyclase-domain-containing-protein	unknown	unknown	gnl|CDD|143636
NZ_AP018042.1|WP_096427798.1|686927_688130_-|delta-aminolevulinic-acid-dehydratase	unknown	unknown	gnl|CDD|239219
NZ_AP018042.1|WP_096427796.1|684661_686065_-|sugar-transferase	unknown	unknown	gnl|CDD|274398
NZ_AP018042.1|WP_096427787.1|666507_667437_-|mechanosensitive-ion-channel	unknown	unknown	gnl|CDD|366370
NZ_AP018042.1|WP_096427791.1|674482_675886_-|MFS-transporter	unknown	unknown	gnl|CDD|340904
NZ_AP018042.1|WP_096427797.1|686218_686965_-|WecB/TagA/CpsF-family-glycosyltransferase	unknown	unknown	gnl|CDD|224833
NZ_AP018042.1|WP_096427793.1|679463_680786_+|DEAD/DEAH-box-helicase	unknown	unknown	gnl|CDD|223587
NZ_AP018042.1|WP_096427799.1|688250_689330_-|hypothetical-protein	unknown	unknown	gnl|CDD|200558
NZ_AP018042.1|WP_096427789.1|669624_670794_-|MFS-transporter	unknown	unknown	gnl|CDD|341031
NZ_AP018042.1|WP_096433344.1|671197_673288_+|TIGR01666-family-membrane-protein	unknown	unknown	gnl|CDD|130727
NZ_AP018042.1|WP_096427782.1|660713_661094_-|VOC-family-protein	unknown	unknown	gnl|CDD|272886
NZ_AP018042.1|WP_096427785.1|665064_665346_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_162845371.1|667858_669079_-|cellulase-family-glycosylhydrolase	unknown	unknown	gnl|CDD|365907
NZ_AP018042.1|WP_096427795.1|683755_684547_-|polysaccharide-biosynthesis/export-family-protein	unknown	unknown	gnl|CDD|224512

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096433346.1|676315_677338_-|L-threonine-3-dehydrogenase	gnl|CDD|180054	PRK05396, tdh, L-threonine 3-dehydrogenase; Validated.	0
NZ_AP018042.1|WP_096427784.1|663540_664506_-|PstS-family-phosphate-ABC-transporter-substrate-binding-protein	gnl|CDD|223304	COG0226, PstS, ABC-type phosphate transport system, periplasmic component [Inorganic ion transport and metabolism].	1.02794e-29
NZ_AP018042.1|WP_188115969.1|680795_681206_-|hydrolase	gnl|CDD|223635	COG0561, Cof, Predicted hydrolases of the HAD superfamily [General function prediction only].	6.37127e-07
NZ_AP018042.1|WP_162845372.1|673300_674371_-|2Fe-2S-iron-sulfur-cluster-binding-domain-containing-protein	gnl|CDD|131215	TIGR02160, 12-phenylacetyl-CoA_epoxidase_subunit_E, phenylacetate-CoA oxygenase/reductase, PaaK subunit. Phenylacetate-CoA oxygenase is comprised of a five gene complex responsible for the hydroxylation of phenylacetate-CoA (PA-CoA) as the second catabolic step in phenylacetic acid (PA) degradation. Although the exact function of this enzyme has not been determined, it has been shown to be required for phenylacetic acid degradation and has been proposed to function in a multicomponent oxygenase acting on phenylacetate-CoA. [Energy metabolism, Other].	4.26317e-79
NZ_AP018042.1|WP_096427794.1|681322_683710_-|polysaccharide-biosynthesis-tyrosine-autokinase	gnl|CDD|349772	cd05387, BY-kinase, bacterial tyrosine-kinase. Bacterial tyrosine (BY)-kinases catalyze the autophosphorylation on a C-terminal tyrosine cluster and also phosphorylate endogenous protein substrates by using ATP as phosphoryl donor. Besides their capacity to function as tyrosine kinase, most of these proteins are also involved in the production and transport of exopolysaccharides. BY-kinases are involved in a number of physiological processes ranging from stress resistance to pathogenicity.	2.46144e-53
NZ_AP018042.1|WP_096427795.1|683755_684547_-|polysaccharide-biosynthesis/export-family-protein	gnl|CDD|224512	COG1596, Wza, Periplasmic protein involved in polysaccharide export, contains SLBB domain of b-grasp fold [Cell wall/membrane/envelope biogenesis].	3.91081e-20
NZ_AP018042.1|WP_096427783.1|661531_662596_-|adenylate/guanylate-cyclase-domain-containing-protein	gnl|CDD|143636	cd07302, CHD, cyclase homology domain. Catalytic domains of the mononucleotidyl cyclases (MNC's), also called cyclase homology domains (CHDs), are part of the class III nucleotidyl cyclases. This class includes eukaryotic and prokaryotic adenylate cyclases (AC's) and guanylate cyclases (GC's). They seem to share a common catalytic mechanism in their requirement for two magnesium ions to bind the polyphosphate moiety of the nucleotide.	7.66289e-27
NZ_AP018042.1|WP_096427798.1|686927_688130_-|delta-aminolevulinic-acid-dehydratase	gnl|CDD|239219	cd02889, SQCY, Squalene cyclase (SQCY) domain; found in class II terpene cyclases that have an alpha 6 - alpha 6 barrel fold. Squalene cyclase (SQCY) and 2,3-oxidosqualene cyclase (OSQCY) are integral membrane proteins that catalyze a cationic cyclization cascade converting linear triterpenes to fused ring compounds. Bacterial SQCY catalyzes the convertion of squalene to hopene or diplopterol. Eukaryotic OSQCY transforms the 2,3-epoxide of squalene to compounds such as, lanosterol (a metabolic precursor of cholesterol and steroid hormones) in mammals and fungi or, cycloartenol in plants. Deletion of a single glycine residue of Alicyclobacillus acidocaldarius SQCY alters its substrate specificity into that of eukaryotic OSQCY. Both enzymes have a second minor domain, which forms an alpha-alpha barrel that is inserted into the major domain. This group also contains SQCY-like archael sequences and some bacterial SQCY's which lack this minor domain.	3.94891e-05
NZ_AP018042.1|WP_096427796.1|684661_686065_-|sugar-transferase	gnl|CDD|274398	TIGR03025, EPS_sugtrans, exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase. Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.	2.34291e-124
NZ_AP018042.1|WP_096427787.1|666507_667437_-|mechanosensitive-ion-channel	gnl|CDD|366370	pfam00924, MS_channel, Mechanosensitive ion channel. Two members of this protein family of M. jannaschii have been functionally characterized. Both proteins form mechanosensitive (MS) ion channels upon reconstitution into liposomes and functional examination by the patch-clamp technique. Therefore this family are likely to also be MS channel proteins.	2.72099e-18
NZ_AP018042.1|WP_096427791.1|674482_675886_-|MFS-transporter	gnl|CDD|340904	cd17346, MFS_DtpA_like, Dipeptide and tripeptide permease A (DtpA)-like subfamily of the Major Facilitator Superfamily of transporters. The DtpA-like subfamily includes four Escherichia coli proteins: dipeptide and tripeptide permeases A (DtpA, TppB or YdgR), B (DtpB or YhiP), C (DtpC or YjdL), and D (DtpD or YbgH). They are proton-dependent permeases that transport di- and tripeptides. DtpA and DtpB display a preference for di- and tripeptides composed of L-amino acids. DtpC shows higher specificity for dipeptides compared to tripeptides, and prefers dipeptides containing a C-terminal lysine residue. The DtpA-like subfamily belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	6.54916e-54
NZ_AP018042.1|WP_096427793.1|679463_680786_+|DEAD/DEAH-box-helicase	gnl|CDD|223587	COG0513, SrmB, Superfamily II DNA and RNA helicases [DNA replication, recombination, and repair / Transcription / Translation, ribosomal structure and biogenesis].	4.56279e-84
NZ_AP018042.1|WP_096427799.1|688250_689330_-|hypothetical-protein	gnl|CDD|200558	cd10932, CE4_u8, Putative catalytic domain of uncharacterized bacterial proteins from the carbohydrate esterase 4 superfamily. This family corresponds to a group of uncharacterized bacterial proteins with high sequence similarity to the catalytic domain of the six-stranded barrel rhizobial NodB-like proteins, which remove N-linked or O-linked acetyl groups from cell wall polysaccharides and belong to the larger carbohydrate esterase 4 (CE4) superfamily.	4.3655e-78
NZ_AP018042.1|WP_096427789.1|669624_670794_-|MFS-transporter	gnl|CDD|341031	cd17478, MFS_FsR, Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters. Fosmidomycin resistance protein (FsR) confers resistance against fosmidomycin. It shows sequence similarity with the bacterial drug-export proteins that mediate resistance to tetracycline and chloramphenicol. This FsR family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.50334e-79
NZ_AP018042.1|WP_096433344.1|671197_673288_+|TIGR01666-family-membrane-protein	gnl|CDD|130727	TIGR01666, Uncharacterized_protein_HI_1680, TIGR01666 family membrane protein. This model represents a clade of sequences from gamma and beta proteobacteria. These proteins are >700 amino acids long and many have been annotated as putative membrane proteins. The gene from Salmonella has been annotated as a putative efflux transporter. The gene from E. coli has the name yccS. [Cell envelope, Other].	1.12258e-137
NZ_AP018042.1|WP_096427782.1|660713_661094_-|VOC-family-protein	gnl|CDD|272886	TIGR00068, Lactoylglutathione_lyase, lactoylglutathione lyase. Lactoylglutathione lyase is also known as aldoketomutase and glyoxalase I. Glyoxylase I is a homodimer in many species. In some eukaryotes, including yeasts and plants, the orthologous protein carries a tandem duplication, is twice as long, and hits this model twice. [Central intermediary metabolism, Amino sugars, Energy metabolism, Other].	1.75989e-32
NZ_AP018042.1|WP_096427797.1|686218_686965_-|WecB/TagA/CpsF-family-glycosyltransferase	gnl|CDD|224833	COG1922, WecG, Teichoic acid biosynthesis proteins [Cell envelope biogenesis, outer membrane].	5.02601e-90
NZ_AP018042.1|WP_096427792.1|677567_679010_-|site-specific-DNA-methyltransferase	gnl|CDD|225100	COG2189, COG2189, Adenine specific DNA methylase Mod [DNA replication, recombination, and repair].	2.26966e-28
NZ_AP018042.1|WP_162845371.1|667858_669079_-|cellulase-family-glycosylhydrolase	gnl|CDD|365907	pfam00150, Cellulase, Cellulase (glycosyl hydrolase family 5).	1.21687e-26

Protein sequences

>NZ_AP018042.1|WP_096427791.1|674482_675886_-|MFS-transporter
MSSEKEKTIKEKFSKAFWVANSVELLERAAYYGVFIVITIYLSRILSFTDVEAALISGLFAGGLYLLPTFSGALADKMGFKNALLLAFSLLSLGYAGLAIFPTLLESAGLVEYSKITVFTGLETSSHKWFIIPIVILIMIGGSFIKSVITGTVAKETTVANRARGFSIFYTMVNIGAFSGKTIVKPLREAMGNEGLITLNYFSAGATLLGLVAVYFFYKSSSSEGEGKSMSEIWAALLKVLTNGRLVALILIVTGFWMVQHQLYATMPKYVLRMAGEGASPSWYANVNPLIVFLFVGLITQLMRKKTALFSMTVGMFIMPVSALCMASGNLLDVESINLGLFSLHPVAFMMIIGIVFQGFAESFISPRFLEYFSLQAPKGEEGMYLGFSHLHSFISSIIGFGLSGYLLSKYCPDPKLFANHAEWELASAHAHYIWYYFVAIATTSAIALIVYGRITRKLDAQNELAA
>NZ_AP018042.1|WP_162845372.1|673300_674371_-|2Fe-2S-iron-sulfur-cluster-binding-domain-containing-protein
MKQEFYRIPIFSIHKPIKEAVTVSFQIPDELLSIFKFDPGQHLCFRFVVNDKEELRMYSLHNSLYEKGLYQVSVKIQKDGLVSNHVANNLNVGDILEISKPQGDFMLSPNSKVAKTYYFFAAGSGITPIYSMLKSILLAEPKSKVFLLYGNRNLPEILFHEELLEWQAKFPDQLRITQTLSKRFLDFSLAPWNGKRGRIDEDMVEAFILENPSTTQISEYYICGPDGMNQMIENTLIDKGIPTKNIHFEYFSAQEIDYEEGLKSEKNATIEASIRSEYFQVQLAEDETILEGLIRIGAPAPYFCKSGICGTCKAHLIEGEVQMKTSMALSAEEVKNNMILACQSLAKTPKVKIEFE
>NZ_AP018042.1|WP_096433344.1|671197_673288_+|TIGR01666-family-membrane-protein
MIKNLWRYPNRLLAIKAAIAMSLLLIPFVVNGSAFMGVTLALGALAGALSETDDHPKGRVKALAITIISFIISSLSVELLLPHPILFGIGLIGSTITFIVVGGLSERYRGVTFGALLVGIYTMLGASISPDWYWQPMLLPAGALCYGILSLILLILRPNRLIEEQLAQGFEDLSLYMKEKARLFPSDAKSQEKIRGRLATLNIQFINSLERSKNVLNSYSDVLDDHNVLRPYLHQFVLLQSLHERASSSHERYDLLSDEPDNIEILEGLGQLLLQLSLACHEVSKSLLMGTTYHHSISLKWTISALKDKIKKQNSTAKSTNLSLLLQNLTQSHFSLQNMNYNLHSGLLPKVDRDPKSIRQKLREQLSWSHPRFRYAIRLSTCFIIGYILILTFDLEKGYWILLTSLFVCQPSYSETRQRLFQRIIGTLSGVVAGVLIVQILPTIAGQVLLLLVAAYAFFVWLRKNYAISVVFVTILVIAAFNLIAGRGVVVMEARVMDTLIGALLAIIVVRFMWPDWQYKHLPGLLSSALEKNRNYFSSILLEYKESNEDDLDYRVARHRANQADNALALSWRGMKLEPKRHQKFQKHAFSLTYLNHALLSYLSALGAHRGIKNYISDDQWEMFHNIEKELSKAVTSMQKLEACDSTQSLTKFIDDLGHKITELERGTERQKFVILYNIAEVTEQLLQEASQLAKE
>NZ_AP018042.1|WP_096427789.1|669624_670794_-|MFS-transporter
MKEEQSSKKFQSNNVILVAASHMLHDIYSSFLAPLRPLLIEKFGVTLAMASLWDLIMRIPWFLNPIIGIIAERTAARYFIIVTPAITAIAMSLLGLAPSFAIISILLFTMGISSAVFHVPAPTMVKRLSGKLTGRGMSYYMFGGELARTAGPLIITASVSYWGLEGTWRLIPFGLIASFILFLKLKNIKISDEIKTKKAEKKYVLSTLKKYLPFFLVLIGITLFRAVMKSGLSAFLPTFYYSERGESLWFANSALAVFQLAGALGTILAGSISDRIGRRNTLLIASIMSPIMMFLFINSVGWLSFVFLVLLGFFIFAPGPVLIALVQDRSKEFPVFMNSIYMTVSFITAALAVFFAGLMGDWIGLEKTYLISSVLSLGAIPFVLWLNKK
>NZ_AP018042.1|WP_162845371.1|667858_669079_-|cellulase-family-glycosylhydrolase
MKIWMFLILIVSAASCQITEKKYAFVEGENIYSPQGELLHLKGVNLGNWLLPEGYMFKLQDCNSPRKIDQAIRELIGNSATTAFWDAFLENYISEADIKWLSENGVNTIRLPFDYRLLTHDDFLGRDMHGYKYLDKAIDWCEKYDIYVLLDMHGAPGGQTGDNIDNSDGYPWLMVDEGMKQQTCDIWQDLAKRYVNNTTVIGYNLLNEPIPHFFDNDSLKPYLEPLYKRITKAIREVDEHHIVFLGGAVWETDFSVFSEPFDDKLAYTFHKYWMPPVQKEIQQFIDFRDKYNVPILMGESGENEDEWVKDFRELLDLNKIHWTFWPYKKMDNTRGPMNFNKPGAYDNFIRYAESDRRTFSKVRALKDSLEGIKPEEVKELLNQFVENSKFENCYPNKGFCDALGLN
>NZ_AP018042.1|WP_096427787.1|666507_667437_-|mechanosensitive-ion-channel
MRKFTRLILSIVLLTFLALYTQIFSFLNESNFWLSSIVISVKYILILACSSWILIEIVRILKHRLLKRFDISYEDNLKSRKLHTQINLLEKVIVFVIILITIGLILISIESIKEIGIGIFASAGVVGIILGLSAQKIVGAVLAGIQIAITQPFRIDDAVVVENEWGWIEEINLTYIVVRIWDKRRLVLPTSYFLEKPFQNWTRTTADILGTVFIYTDYTISVEALREELARLLKDCELWDKKVNVIQVTDSKENYMELRILVSAKNSPTAWDLRVFIREKMIEFIQKNYPESLPKTRVNIENKECPKIQ
>NZ_AP018042.1|WP_096427785.1|665064_665346_-|hypothetical-protein
MNKEEFKAKANQTIDEVSAKINEFKAKKESTKDDVKSKYEETLKDLETKKADLKAKYSELENATDDKWEDVKNAFSSAADSFKEGFSKIASLF
>NZ_AP018042.1|WP_096427784.1|663540_664506_-|PstS-family-phosphate-ABC-transporter-substrate-binding-protein
MKAIRCVFIFIIFLTIGCVNKTPQQQQEKESLPTHKGEINIKGAYALMPLVQRWITEYQKIHPLVLFNLSPVGSGEVLSEIYSDKTDLLMISSELPDKLESAVWILPVAKLSVVMIVNKKNPYWSQILEAGIKKDDMSKLFTGEITSWGNLFGEPGKQPVSVYFRSDQAGATDILSKFLWLDDQKMSGTGILGENQLIEAIKGDSLAIGYCNFIYSFDPESKEFRDDIGIIPLDLNQNGSLDMKENFYENFTELQRAMWLGKYPCILNRPLQFVASQKPSTKELYDFLKWTIIDGQKIVPEMGYMELRSSEIQRCLSYIEN
>NZ_AP018042.1|WP_096427783.1|661531_662596_-|adenylate/guanylate-cyclase-domain-containing-protein
MRTNLREWLIIILSFILVLYMYAIFSYFGVEDFIQEGVLKEYFDTNIWHLEVIFSGILLGILFILVNQLTEKQIFRRKSFGFNILLKSALYLIGLIIVCLLIFELFLFFELVPQEQMEKFQALLSAKFYISGVLYYASFILILNFILYINQKLGPGLLIDLLTGKYYHPRNEELIFLFVDLKNSTSLAEKLGHIEYSKFIKECVHELTPVIQKFNARVYQYAGDEVILFWSKKEGFRELNCLNAFFEFSDILNRQKEYFKSKYGEIPIFKAGMDVGIVTATEIGDIKREIAYHGDVLNTAARLEKKCNEFNEKLIITQNVIDQIQSANGYNFKLLSDLPLRGKTENIKFYSVKS
>NZ_AP018042.1|WP_096427782.1|660713_661094_-|VOC-family-protein
MKYKLEHACIRVMDLDKSVDFYKKALSLEEVKRLDYPDYKFTLVYLSDENKNFEIEITHNYDTEKPYEIGNGFSHFALTVSDLEGSREFHKNMGIETTDLKGLPGEKPKYYFITDPDGYKIEIIRS
>NZ_AP018042.1|WP_096433346.1|676315_677338_-|L-threonine-3-dehydrogenase
MRALVKSKAEKGLWMEEVDVPKVGVNDVLVKIRKTAICGTDLHIYKWDEWAQNTIKPGMIIGHEYVGEVTEMGAGVTGFKIGDRVTGEGHIACGHCRNCRRGRKHVCEHTVGIGVNIDGIFAEYAAIPSSNLMKMSDEISDELLAIMDPFGNATHTTLSWQIIGEDVLVTGAGLIGSMCVAIAKFAGARYVVATETNPYRIELAKKMGADRVVNPLTESLDDAIKDLGMIGFDIGLECSGAPAAFSDMVSHMYNSGKISLLGILPAQTKVNWNDIIFKGLQLKGIYGREMYETWYQMEQMLMSGLDLSPMISHRFKADEFQKGFDIMEEGNCGKVILDWE
>NZ_AP018042.1|WP_096427792.1|677567_679010_-|site-specific-DNA-methyltransferase
MQGKKLLSFYQKQKKITELKSLLPEVFVGEQLDIQKLKLYLEADQFQDELNYGLSWFGKDTSLQQANEKTTDKLVFDKAKSLRPNKTKNLFVEGDNLDALRLLQKDYVGKIKLIYVDPPYNTGNDFGYNDRFKIRSNQYLEFLNGSGINRVDSFLKNQIESGEVHTNWLNMIYPRLVLSRQLLTDNGAIAISIDESEKPNMQLVCNEIFGEENFVGCFIWVNRSNSSASSNSFAANHEYILIYAKDSKEIKLKGEPKDLSNYSNPDHDLNGDWIPDNPSAASGNSNSRFTITNPYTQEEYLPPQGRYWAFSEQRVREWFDSGKLIFPKDKGKRFLLKKYKSELKSIYNPISSIITDIPTSKGTRELKELYPEGNPFKYPKPTALLVKIFEQLSDEEDLILDLFAGSASAVHAVFKLDEMQSSNRRFICVQNTEAVKEDSDAFRMGFSAISDLTKDRIVRAGKIFHRLDTGFKFYRIEKTI
>NZ_AP018042.1|WP_096427793.1|679463_680786_+|DEAD/DEAH-box-helicase
MQAKNIDNKKILEKLSIQNLNEMQEECAKAYTKNSDLLLVSPTGSGKTLAFLLPMLAELDPEVNAVQTLILTPARELSLQIEGVFKSMGTNFKVNCCYGGHPMSTEKNNLKTPPAVLIGTPGRIADHIRRGNFDCRNIKTLILDEFDKSLELGFQKEMKEVTLALPSKIKKILTSATNNIDIPDFARLNSLIELDFIPNAKPIALAQKKVISEDKDKLATLFQLVCHLGNQPSLVFCNHRDAVERIGKHFLNEGIQCDVFHGGLEQDDRERALLKFRNGSHNLLIVTDLASRGLDIPEIENVIHYQLPRVEEAFTHRNGRTARMNAAGTSYLVLAKDETPPEFIPEDIEILELTDDKPLPAEPEWETLYICLGRKEKINKIDLVGFFCKIANLRKDEIGKIDVLDHSSFVAVKRGMGEKLARKLKNELIKKKKVKIEVAY
>NZ_AP018042.1|WP_188115969.1|680795_681206_-|hydrolase
MIIAVDFDGTIVQHKYPEIGSEIPFAIESLIALQKEGHQIILWTYRTGGMLFEAVDYCEKRGLEFYCVNSNYPEEEFDDTISRKIYADLYIDDRNFGGLPEWEDIFGSISTQNLVPDKESKTKQMFIRFIDKVFGD
>NZ_AP018042.1|WP_096427794.1|681322_683710_-|polysaccharide-biosynthesis-tyrosine-autokinase
MKHVDEVMDYFDQKDSPDVKEFLFRLLSQWKMFAVCGALGILLAYFISKYSNPVYMMRSSLLIHVDADESSAQSMFEGYKIQDKANIQNHVEILKSYTINRKAMRNLGWNQAWYKKQLFSDRGLYRHEPFEVQQLDGGNITNLPISIVQIDQKTYRIKVDGKVDYKGAEVHVKFEATAYFDREFKNKYFHFLIKTKQQKINKEEHNYFVFNNLDGLTLDYMESMTISVAEKKAELIQLRIDNSEPERGVDYLNELNWVFIQFGLIEKNKKSESTVRFIDSQLEGIVDSLQMAGQSVTNFRSKNRIVDLGQEAGVIVAKVGELESLLSRAQISLDYYKNLLRNLGDANQMKQVIAPSITGVTDPSLDALVAKLTDLYGKREVLSYSVQEKNPSMVILNKEIQLIQQSLKENLQSTVANSRIEVQSLNARMEDVKEQLARMPKHEQKLVSMKRNFDLNNELYTFLLKKRAEAAIAKASNVSDSQIIDPARLATIVKIKPKTAINLLIGMFLGIGLPFLIIILKEFFNDNIQNKEELMKRTQLPVLGTIAHNKYSEAVPVFHHSRSALAESFRTLRTNLNYVLPNNPTKIIGIHSTVPEEGKSFATVNLATIVAMNNKRVLLIGADLRKPKIEVAFDLDNKLGLSTYLISYSNCDEIIQETHIENLYCVTSGPVPPNPAELLENGRFEKFLSEVKGKYDYIFIDNAPLSMVTDGIITGRFADANLFVLRQGFSHRSQIDFVNQLAVKGTLRNVSLVLNDVAMNGYQGKSSMNSSGNGYYYEDTPPGVVRRLWGKVSSN
>NZ_AP018042.1|WP_096427795.1|683755_684547_-|polysaccharide-biosynthesis/export-family-protein
MKKKYLLLMSLVLVMSIISCRSKKDLVYLQDTEAEVLPAEFTTSVPEYRIKTNDNLFVSIKSLNPEVNQLYNPAQSIGGSGGVQQLYSQQSDQYLNGYQVDSNGNISLPILGNVAIAGLSQAEAQAKVQERADEYLKDASVKVKLLNYKVSVMGEVAAPGVYYNYNSSLTVLEAISMANGITEYAKIDRVLVMRTSEKQSETFRLDLTKKDFLKSKAFFLQPNDVVYVEPHKIKNTNMNSAVYSLLLSTVSTAVLITSLILTN
>NZ_AP018042.1|WP_096427796.1|684661_686065_-|sugar-transferase
MIKGREQTLARLSTLMQVGLSLGCYWLVTWAVATYLKPLNIDIHDNNTIYLLIIAIWYTLIKELNLGKMLRVKTYSELFAEYFALVVISSGLLHLGSLFLTESVPTLILALFACLNLFMLFGFRMLSYRIFKMLRGKGYNTRNVLIVADDESYLHFIDRMLFIRDWGYHVWAIMSDGKHIKTKYESHVRVLHEHFQLSEIIDANVVDEVIYCKGKFDNDEVRALIYACSEVGVTFRVYSELLSVVSQRSHLTYFKELPFLTFANHTKNYFALKVKELLDFLFSSAIVIFISPVLLAIAVAIKIEDGGSIFFRQRRVGLNGRIFDCLKFRTMVENAEALRVKLENLNEQSGPVFKIKNDPRVTRVGRFLRKTSLDELPQFFNVLKGEMSVVGPRPPIPSEVQQYQRWQRRRLSMKPGITCIWQVSGRNNIPFEEWMKLDMLYIDTWSLKLDLILFLSTIKVVFTGEGQ
>NZ_AP018042.1|WP_096427797.1|686218_686965_-|WecB/TagA/CpsF-family-glycosyltransferase
MRKRITIMNSPVDVLTMKETLHLIGDSIRDKKPIQHIVVNAAKLVHMQSDTELFKSVVASDIINADGMAVVWASELLGNPLPERVSGVDLMQELVIMAAEKQYKIFFFGGKEEVVSEVVRKYTSVFGEEIIAGYRNGYFNKEQEGDIADQIAESKADILFVAISSPTKEIFLNNYKEQLKVPFIMGVGGSFDVVAGKVKRAPVWMQNSGLEWFYRFLQEPGRMWKRYLTTNSLFLYYIFKERIQKFFK
>NZ_AP018042.1|WP_096427798.1|686927_688130_-|delta-aminolevulinic-acid-dehydratase
MYKASFEKLRAYIESQEYKGWDPYDGLNSKLFQSIPFAKNSRLMRLIWIQAFKKSSVNFRGLARVEKDYNPKGLGLFLSGYCKLYQFDRTQEVRSRIHFLSEKLLEMQSKGWSGPCWGYNFDWQARAFFQPKGTPSVVVTTYVGSALLDAYEILGDKNLLETALRIKDFILKDLNRTYDENKDFCFSYSPLDITQVFNASLLGSRMLSRIYHYTQDEEVLNCAKKSVQYCINHQNSDGSWAYGTLPYHRWIDNFHTGFNLEALSTYRMYAKDSSFDKQIQLGLDYYLATFFADDGTPKYYNNSVYPIDIHCPAQLPITLSSLHVLEENQELVDRVLKWTINNLQDQYGYFYFQSGKRSTNKIPYIRWAQAWMFLSLSNYLVETLKIPEHEKENYNNELAG
>NZ_AP018042.1|WP_096427799.1|688250_689330_-|hypothetical-protein
MISLCLTLDYELFGSGRGDVFKHIIEPTDRLLEICKQHSIKLSIFFEVVEYWKIKEAYENGISMGYSSNPATAMKEQIKRAYSLGHDVQLHLHPQWIDAKYENKEWVLNLEYWRLPEVPDLANETISMGLSELIGKGKQTLESILQAENPDYKCNILRAGGYNIDPSDRLLKVLKEHSFIADSSVYYGGKAEGNLSRYDYSDILESKAYWYASNTSLVSANGAGSGFLELPIYARKMKRFLKYDAVRIRSAMQNKANSIEKFKNNSVKKSKWETIQFLWKKEALTWDFCLFSKGKMRSFLKSANKLQKQSAYLFHPFILVGHPKDFYYPDALLYLIQQAEKGEITFLTLSEMLVKIKQQ

Crispr_ID: NZ_AP018042_4

• CRISPR_ID: NZ_AP018042_4
• CRISPR_location: 1529138-1529322
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 4 spacers

Consensus repeat of NZ_AP018042_4

Consensus_repeat	Method
CCATTATAATTTGTTAANG	CRT

spacers of NZ_AP018042_4

>4.1|1529157|22|NZ_AP018042|CRT
GCTCCACTAAATGATAAAACGT
>4.2|1529198|23|NZ_AP018042|CRT
ACTCCACGAAAGGCTTAGGCGAG
>4.3|1529240|22|NZ_AP018042|CRT
GCTTCACTAAATGATAAAACGT
>4.4|1529281|23|NZ_AP018042|CRT
ACTCCACGAAAGGCTTAGGCAAG

CRISPR arrays and Neighbor proteins around NZ_AP018042_4

CRISPR arrays

The CRISPR arrays of NZ_AP018042_4

>merge|NZ_AP018042|4|1529138-1529322|CRT
TCGTTATAATTTGTTAATGGCTCCACTAAATGATAAAACGTCCATTATAATTTGTTAACGACTCCACGAAAGGCTTAGGCGAGCCATTATAATTTGTTAATGGCTTCACTAAATGATAAAACGTCCATTATAATTTGTTAATGACTCCACGAAAGGCTTAGGCAAGCCATTATAATATGTTAACG

>NZ_AP018042|4|1|1529138-1529322|CRT
TCGTTATAATTTGTTAATG	GCTCCACTAAATGATAAAACGT
CCATTATAATTTGTTAACG	ACTCCACGAAAGGCTTAGGCGAG
CCATTATAATTTGTTAATG	GCTTCACTAAATGATAAAACGT
CCATTATAATTTGTTAATG	ACTCCACGAAAGGCTTAGGCAAG
CCATTATAATATGTTAACG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096428421.1|1531991_1533275_-|phosphopyruvate-hydratase	unknown	unknown	gnl|CDD|234617
NZ_AP018042.1|WP_096428410.1|1518481_1519582_-|redox-regulated-ATPase-YchF	unknown	unknown	gnl|CDD|236583
NZ_AP018042.1|WP_054714084.1|1536700_1536922_-|translation-initiation-factor-IF-1	unknown	unknown	gnl|CDD|178954
NZ_AP018042.1|WP_096428422.1|1533470_1534004_-|50S-ribosomal-protein-L17	unknown	unknown	gnl|CDD|235522
NZ_AP018042.1|WP_096428423.1|1534010_1535003_-|DNA-directed-RNA-polymerase-subunit-alpha	unknown	unknown	gnl|CDD|235359
NZ_AP018042.1|WP_054714088.1|1536572_1536689_-|50S-ribosomal-protein-L36	unknown	unknown	gnl|CDD|376334
NZ_AP018042.1|WP_096428424.1|1535023_1535632_-|30S-ribosomal-protein-S4	unknown	unknown	gnl|CDD|235411
NZ_AP018042.1|WP_096428420.1|1531410_1531920_+|response-regulator	unknown	unknown	gnl|CDD|381099
NZ_AP018042.1|WP_096433452.1|1529515_1531204_-|energy-dependent-translational-throttle-protein-EttA	unknown	unknown	gnl|CDD|236992
NZ_AP018042.1|WP_096428416.1|1525798_1526269_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096428414.1|1524787_1525345_+|RNA-polymerase-sigma-factor	unknown	unknown	gnl|CDD|274357
NZ_AP018042.1|WP_096428411.1|1519760_1520804_+|hypothetical-protein	unknown	unknown	gnl|CDD|227604
NZ_AP018042.1|WP_096428419.1|1528350_1528704_-|DUF4870-domain-containing-protein	unknown	unknown	gnl|CDD|378240
NZ_AP018042.1|WP_096428426.1|1536157_1536535_-|30S-ribosomal-protein-S13	unknown	unknown	gnl|CDD|235358
NZ_AP018042.1|WP_096428417.1|1526334_1527702_-|histidine--tRNA-ligase	unknown	unknown	gnl|CDD|234586
NZ_AP018042.1|WP_096428409.1|1517956_1518334_-|fluoride-efflux-transporter-CrcB	unknown	unknown	gnl|CDD|172690
NZ_AP018042.1|WP_145957575.1|1525407_1525788_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_145957574.1|1521100_1522420_+|VWA-domain-containing-protein	unknown	unknown	gnl|CDD|238733
NZ_AP018042.1|WP_096428425.1|1535757_1536147_-|30S-ribosomal-protein-S11	unknown	unknown	gnl|CDD|180007
NZ_AP018042.1|WP_096428413.1|1522576_1524637_+|transketolase	unknown	unknown	gnl|CDD|223100

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096428421.1|1531991_1533275_-|phosphopyruvate-hydratase	gnl|CDD|234617	PRK00077, eno, enolase; Provisional.	0
NZ_AP018042.1|WP_096428410.1|1518481_1519582_-|redox-regulated-ATPase-YchF	gnl|CDD|236583	PRK09601, PRK09601, redox-regulated ATPase YchF.	0
NZ_AP018042.1|WP_054714084.1|1536700_1536922_-|translation-initiation-factor-IF-1	gnl|CDD|178954	PRK00276, infA, translation initiation factor IF-1; Validated.	1.05766e-49
NZ_AP018042.1|WP_096428422.1|1533470_1534004_-|50S-ribosomal-protein-L17	gnl|CDD|235522	PRK05591, rplQ, 50S ribosomal protein L17; Validated.	1.75697e-57
NZ_AP018042.1|WP_054714088.1|1536572_1536689_-|50S-ribosomal-protein-L36	gnl|CDD|376334	pfam00444, Ribosomal_L36, Ribosomal protein L36.	6.77484e-12
NZ_AP018042.1|WP_096428424.1|1535023_1535632_-|30S-ribosomal-protein-S4	gnl|CDD|235411	PRK05327, rpsD, 30S ribosomal protein S4; Validated.	1.33419e-120
NZ_AP018042.1|WP_096428420.1|1531410_1531920_+|response-regulator	gnl|CDD|381099	cd17546, REC_hyHK_CKI1_RcsC-like, phosphoacceptor receiver (REC) domain of hybrid sensor histidine kinases/response regulators similar to Arabidopsis thaliana CKI1 and Escherichia coli RcsC. This family is composed of hybrid sensor histidine kinases/response regulators that are sensor histidine kinases (HKs) fused with a REC domain, similar to the sensor histidine kinase CKI1 from Arabidopsis thaliana, which is involved in multi-step phosphorelay (MSP) signaling that mediates responses to a variety of important stimuli in plants. MSP involves a signal being transferred from HKs via histidine phosphotransfer proteins (AHP1-AHP5) to nuclear response regulators. The CKI1 REC domain specifically interacts with the downstream signaling protein AHP2, AHP3 and AHP5. The plant MSP system has evolved from the prokaryotic two-component system (TCS), which allows organisms to sense and respond to changes in environmental conditions. This family also includes bacterial hybrid sensor HKs such as Escherichia coli RcsC, which is a component of the Rcs signalling pathway that controls a variety of physiological functions like capsule synthesis, cell division, and motility. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.	1.07289e-40
NZ_AP018042.1|WP_096433452.1|1529515_1531204_-|energy-dependent-translational-throttle-protein-EttA	gnl|CDD|236992	PRK11819, PRK11819, putative ABC transporter ATP-binding protein; Reviewed.	0
NZ_AP018042.1|WP_096428414.1|1524787_1525345_+|RNA-polymerase-sigma-factor	gnl|CDD|274357	TIGR02937, RNA_polymerase_sigma_factor, RNA polymerase sigma factor, sigma-70 family. This model encompasses all varieties of the sigma-70 type sigma factors including the ECF subfamily. A number of sigma factors have names with a different number than 70 (i.e. sigma-38), but in fact, all except for the Sigma-54 family (TIGR02395) are included within this family. Several Pfam models hit segments of these sequences including Sigma-70 region 2 (pfam04542) and Sigma-70, region 4 (pfam04545), but not always above their respective trusted cutoffs.	4.76128e-31
NZ_AP018042.1|WP_096428411.1|1519760_1520804_+|hypothetical-protein	gnl|CDD|227604	COG5279, CYK3, Uncharacterized protein involved in cytokinesis, contains TGc (transglutaminase/protease-like) domain [Cell division and chromosome partitioning].	9.09577e-23
NZ_AP018042.1|WP_096428419.1|1528350_1528704_-|DUF4870-domain-containing-protein	gnl|CDD|378240	pfam09685, DUF4870, Domain of unknown function (DUF4870).	9.3567e-40
NZ_AP018042.1|WP_096428426.1|1536157_1536535_-|30S-ribosomal-protein-S13	gnl|CDD|235358	PRK05179, rpsM, 30S ribosomal protein S13; Validated.	6.74761e-73
NZ_AP018042.1|WP_096428417.1|1526334_1527702_-|histidine--tRNA-ligase	gnl|CDD|234586	PRK00037, hisS, histidyl-tRNA synthetase; Reviewed.	6.92513e-146
NZ_AP018042.1|WP_096428409.1|1517956_1518334_-|fluoride-efflux-transporter-CrcB	gnl|CDD|172690	PRK14202, PRK14202, fluoride efflux transporter CrcB.	2.38841e-33
NZ_AP018042.1|WP_096428423.1|1534010_1535003_-|DNA-directed-RNA-polymerase-subunit-alpha	gnl|CDD|235359	PRK05182, PRK05182, DNA-directed RNA polymerase subunit alpha; Provisional.	1.70788e-151
NZ_AP018042.1|WP_145957574.1|1521100_1522420_+|VWA-domain-containing-protein	gnl|CDD|238733	cd01456, vWA_ywmD_type, VWA ywmD type:Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of fold. The vWA domain, since its discovery, has drawn great interest because of its widespread occurrence and its involvement in a wide variety of important cellular functions. These include basal membrane formation, cell migration, cell differentiation, adhesion, haemostasis, signaling, chromosomal stability, malignant transformation and in immune defenses In integrins these domains form heterodimers while in vWF it forms multimers. There are different interaction surfaces of this domain as seen by the various molecules it complexes with. Ligand binding in most cases is mediated by the presence of a metal ion dependent adhesion site termed as the MIDAS motif that is a characteristic feature of most, if not all A domains. Not much is known about the function of the members of this subgroup. All members of this subgroup however have a conserved MIDAS motif. .	1.21129e-16
NZ_AP018042.1|WP_096428425.1|1535757_1536147_-|30S-ribosomal-protein-S11	gnl|CDD|180007	PRK05309, PRK05309, 30S ribosomal protein S11; Validated.	7.81264e-78
NZ_AP018042.1|WP_096428413.1|1522576_1524637_+|transketolase	gnl|CDD|223100	COG0021, TktA, Transketolase [Carbohydrate transport and metabolism].	0

Protein sequences

>NZ_AP018042.1|WP_096428419.1|1528350_1528704_-|DUF4870-domain-containing-protein
MNQFATPTDEKNWAMFCHLSSFAGVIIPFGNVVGPLILWSMKKDYSELVDREGKKSINFQISMSIYIFVSAILVFVGIGILLLIGLALVNLIFVVMAIVKTLNGEDYEYPLSIKFLN
>NZ_AP018042.1|WP_096428417.1|1526334_1527702_-|histidine--tRNA-ligase
MAQKPSIPKGTRDFSPVEMVKRNYIFDTIKEVFQLYGFMPIETPSMENLSTLMGKYGEEGDKLLFKVLNSGDYLSKINEDQIREKNSVKLATKISEKGLRYDLTVPFARYVVQHRNEIDFPFKRYQIQPVWRADRPQKGRYREFFQCDVDVIGSNSLLNEVELIQIVDQVYKRLQLNVVVKLNNRKILAGIAEIIGEAEKIVDITVAIDKLDKIGLENVNKELVDKGIPQEAIDKLQPIILLSGSNSEKLAKLRELLSSSEVGMSGIEELETVFTYLSQIDVKTEVELDLTLARGLNYYTGAIFEVKSKDMEFGSITGGGRYDDLTGIFGLKDVSGVGISFGADRIYDVLEQMDKFPTNTGITTKVLFINFGKKEEAFCLPILAKLRSNGINAEIFPDSAKMKKQMTYANNKNIPFVILVGESEVEEGVISLKNMESGDQEKLTPDQLIDRLVLS
>NZ_AP018042.1|WP_096428416.1|1525798_1526269_+|hypothetical-protein
MNRTLIILTLVLTFSTSGIAQEKEHGMHHQMSERFEAQKISFITQKLELTPDEAQLFWPLYNELGKKKKENREQAKTLFRKLRNNSEELSDKELGDISDELIELKLQEAHLQKEYHLKYKKILSAKKILTLYHSERQFQSMLLRQIKERGKHQHEK
>NZ_AP018042.1|WP_145957575.1|1525407_1525788_+|hypothetical-protein
MKNDQPFKVPEDYFENLSDRVQERITTEENPNKRLLQVLKPYFWMAASIIGIVFIAKIVLTTSVSNDYKIQQISQTESTAIDTQKTSTGLNELDWFSDETTEATSDEIIEYLSDFDIDSESLLANL
>NZ_AP018042.1|WP_096428414.1|1524787_1525345_+|RNA-polymerase-sigma-factor
MNNKSDEEILELFRDKNTQSQAFSFLVKKYQERLYWQIRKIVLLHDDTDDVLQNTFVKIWKGLQNFRSDSQLFTWIFRIATNEALTFLKDKQKKHLGYANSEVEDMLLANLEADPYFDANKAQLELQKAILKLPEKQRLVFNMKYFDDLKYTEMSEILETSVGALKASYHHAVKKIESFLSLNEV
>NZ_AP018042.1|WP_096428413.1|1522576_1524637_+|transketolase
MDLKEKKNRVDLTATDMAANNVRILSAAMVEKAKSGHPGGAMGGADFINVLYSEFLNFDPTDMNWINRDRFFLDPGHMSPMLYSILSLTGNYTMEELANFRQWGSPTPGHPEVEVARGVENTSGPLGQGHTMAVGAAIAERFLAARFGDWMSHKIYTFISDGGIQEEISQGAGRIAGTLGLSNLIMFYDSNNIQLSTKVEEVTTEDTAKKYEAWNWNVITIKGNDATAIREALAAANKETERPTLIIGKTLMGKGAVTADGTSFENMVSTHGQPIGAAGASIGKTIENLGGNPENPFQIFPEVQEYYAEVLKKKQAFADAKKAEQAEWTKANPELAAKLEKFFSNEAPELDYEAIVQKSGDATRSASSVVLGELAKNVDNMIVSSADLSNSDKTDGFLKNTKALAKGDFSGAFLQAGVAELTMASICNGIALHGGVIAACGTFFVFSDYMKPAVRLAALMQVPVKYIWTHDAFRVGEDGPTHQPIEQEAQIRLLEQLKNHSGQMSLMALRPADADEATVAWKMAMENTKTPTALIFSRQNITKLPSKGNRYQEALQAQKGAYIVEKPETTPDVVLLASGSEVATLVEGAKILRAEKGLNVQIVSVPSEGLFRTQSAEYQNEVIPAGIPVLGLTAGLPVTLLGLVGPLGKSIGLDHFGYSAPASVLDEKFGYTAENVAKEVEALLQK
>NZ_AP018042.1|WP_145957574.1|1521100_1522420_+|VWA-domain-containing-protein
MAFSQVSHEKNEKTRILFIFDASQSMNGYWEESKKINIARRFLIQMIDSLEYEDNVELALRVYGHQSVVPPQDCNDTKLEVPFKPGNAGEIRQTLRYITPKGTTPIAHSLELAANDFPEVNSLTRNVIILITDGVEACDGDPCAVSLELQKKGIFLKPFIIGIGLDVDFKSSFECIGNFIEVKKEERFGGTLEYVISQVLNKTSVQINLIDANGSPTETDVPMTFYNSVSGKVRYQFMHTLNAKGNPDTLFLDPLLTYDITIHTIPELHRDSISIVAGKHTAIGFDAPQGMLKIASARTTLYKDLKILIRKNKEIINIQIPNEVTKYLTGKYDLEVLSLPRISIPDVEINQSKTTSIKIPQPGLVTIFKPTSGPCSLFVQKGSKLEWIHNIPEKTLRETLTLQPGTYHLIFRNKNAYLSQSSRKTSFVITSGKSIPVRL
>NZ_AP018042.1|WP_096428411.1|1519760_1520804_+|hypothetical-protein
MYQKLTIIIILALASLELVQAQEPTFGKYGNIDRYVKRTPDSLSQNIVLLHNYLVAPAESNEDKIRAFYMWIITNIKYEDQVELMYDSNLLFYMGSNNCSSPVCVLKKGKAVCEGFSKLFQFFCTYSGLESYTIGGYISKNGALQDRATHSWNVVKINDEWRFFDLSWANAILHHSGIKSKTNEFYMVSPEEFIVSHLPIIPMWQFLNTPISIQIFNAGEEPIKKYMNSAPANYNYTDTLIQFNRMSGANKRLKTAHEIYRTNPNNKFNLAIEYYRYARNMKNFDGEIDPRNYSRLIKARGKMQLAIDLFKSSSDISSQIMLLQVKADIDNIDRLIISAKKQIRYLK
>NZ_AP018042.1|WP_096428410.1|1518481_1519582_-|redox-regulated-ATPase-YchF
MALQCGIVGLPNVGKSTLFNCLSNAKAQSANFPFCTIEPNVGVISIPDDRLIRLTELVKPQRIQPSVMEIVDIAGLVKGASKGEGLGNKFLSNIRETSAIIHVLRCFEDDNIVHVDGSVNPIRDKETIDIELQLKDLENVEARLQKAAKLAKIGKNPDAVRLTDVLGRYKEVLEQGKSARVVELSEADEKVAADLHLLTTKPILYVCNVDEASVVNGNVHVDAVREAVKNENAEVIVVGAGIEADIAELETYEEKQMFLEDLGLKEPGVNKLIKAAFRLLELETYFTAGVKEVRSWTYPKGAKAPQAAGVIHTDFEKGFIRAEVIKYEDFSNLGSEAACKEAGKMNVEGKEYVVQDGDIMHFRFNV
>NZ_AP018042.1|WP_096428409.1|1517956_1518334_-|fluoride-efflux-transporter-CrcB
MLRTVLLVGLGGFLGSISRFLAEQGLHRYFDTVFPIGTMTVNIVGSFIIGIVYAVAERDNLVSPEMRMFLAVGFCGGFTTFSSFAFDNLNLLKDSGFLYLSLYVTGSVFLGLLAVYFGTQVHKLF
>NZ_AP018042.1|WP_096433452.1|1529515_1531204_-|energy-dependent-translational-throttle-protein-EttA
MADDKKIIFSMDRVSKTFSSQKKVLNNINLSFFYGAKIGIIGLNGSGKSTLLKIIAGLESSFDGHVVWSKEHNIGYLAQEPILDDSKTVKEIVEEGVQEIVDTLNAYEAVNLKFGDPEVLENADKMQAVMDEQADLQEKIDHFDAWNLDTKLERAMDALRCPEGDELIKNCSGGERRRVALCRLLLQEPDILLLDEPTNHLDAESVEWLEQHLSQYKGTVISITHDRYFLDNVAGWILELDRGEGIPWKGNYSAWLDQKTQRMAMEEKTASKRRKTLERELEWVRMAPKARQAKSKARLSAYDSMMNEDLKDKEEKLEIFIPNGPRLGNKVIQAQAVSKAFGSKLLYDDLEFELPPAGIVGVIGPNGAGKTTLFRMIMDLEKPDKGTFEVGDTVKVGYVDQQHADIDPEKSVYEVISGGGDLINVGGRTVNARAYVGRFNFNGADQEKKCGVLSGGERNRLHLALTLKEEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHDRWFLDRIATHILAFEGNSHVQFFEGGYSDYEINRKKRMGDEGPKRIRYKKLLAD
>NZ_AP018042.1|WP_096428420.1|1531410_1531920_+|response-regulator
MERTANKKLSELLEQIGIETKKKIYWEETIDSSVVVKTHPNKRNVPIKDAKILLVEDQPSNRKTIEVYLKQKVGSVDIAENGKQAVELFEKEQYDIVLMDLQMPIMGGIEASLRMRKIEEEREFSIPIIAFTANFYSDDKEVSIEVGMDAYLSKPFQCKTLFGLMQEYI
>NZ_AP018042.1|WP_096428421.1|1531991_1533275_-|phosphopyruvate-hydratase
MSEIVKVHGREILDSRGNPTIEVEVTLASGYMGRAGVPSGASTGENEALELRDGDKLRYLGKGVLKAVENVNNVIAPVIMGMDATNQVAIDKAMIALDGTKTKSKLGANAMLGVSLAVAKAAAEYSELPLYRYIGGANANVLPVPMMNIINGGSHSDATIAFQEFMIRPIGAPSFREALRMGAEVFHALAKVLKGKGLSTAVGDEGGFAPMLGGTEEAIECILTAIKNAGYKAGRKEDGGDVSIAMDCAASEFFKEGVYDYSLFEPNGAKRNSDEQAAYLAELISKYPIDSIEDGMDEGDWDGWVALNALIGDKCQIVGDDLFVTNVDYLAKGIKLKAANSILIKVNQIGTLTETLNAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGENAIFGC
>NZ_AP018042.1|WP_096428422.1|1533470_1534004_-|50S-ribosomal-protein-L17
MRHRKKFNHLGRKTAHRKAMLANMASSLILHKRISTTTAKAKALKMYVEPLISKSKVDSTHSRRIVFGYLKQKEAVTELFREVSPKITNRNGGYTRILKTGNRLGDNAEMCMVELVDFNETYLTEKKAVAKKSTRRKRGGSKASEEVATEVSEVKAEEVKADKAPEASTEATKEEEK
>NZ_AP018042.1|WP_096428423.1|1534010_1535003_-|DNA-directed-RNA-polymerase-subunit-alpha
MAILAFQKPDKVIMLDADNKFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITTIKIQGVDHEFSTIPGVIQDVTEIILNLKQVRFKQKVEEVDSELVTITISDQDTFTAGDINKFLTGFEVLNPELVICKMDSSAKLQMDLAINKGRGYVPSVENKPVDVEFGVIPIDSIFTPIKNVKYAVENYRVEQKTDYEKLVFDITTDGSIHPKDALKEAAKILIYHFMLFSDEKITLDSDEKFANEEFDEEVLHMRQLLKTKLVDMDLSVRALNCLKAADVDTLGDLVQFNRNDLLKFRNFGKKSLTELDDLLVSLNLTFGMDISKYKLDKE
>NZ_AP018042.1|WP_096428424.1|1535023_1535632_-|30S-ribosomal-protein-S4
MARYIGPKSRIARKFGEAIFGPDKAFENKNYPPGQHGNSRRRKKMSEYGVQLKEKQKAKYTYGVLEKQFSNLFEKAARSKGITGEVLLQLLECRLDNVIFRLGVAPTRSAARQLVSHKHVTVNGQVCNIPSLSVKSGDIIGIREKSKSLEVITDSLSTARYNQSSWLEWDQTSLSGKFLNTPERTEIPENIKEQLIVELYSK
>NZ_AP018042.1|WP_096428425.1|1535757_1536147_-|30S-ribosomal-protein-S11
MAKKSVSVKKRVVKIEPVGQAHIHSSFNNIIISLTNSTGQVISWSSAGKMGFRGSKKNTPYAAQQAATDCGKVAHDLGLRKVKVYVKGPGNGRESAIRAINSCGIDIAEIVDVTPLPHNGCRPPKRRRV
>NZ_AP018042.1|WP_096428426.1|1536157_1536535_-|30S-ribosomal-protein-S13
MARIVGVDLPQNKRGIISLTYIYGIGRSAARQILDEAEVPHDKQVKDWTDEESNRIRQAINANLKVEGELRSLNQLNIKRLMDIGCYRGIRHRIGLPVRGQRTKNNSRTRKGKRKTVANKKKATK
>NZ_AP018042.1|WP_054714088.1|1536572_1536689_-|50S-ribosomal-protein-L36
MKVRASVKKRSEDCKIVRRKGRLYVINKKNPKFKQRQG
>NZ_AP018042.1|WP_054714084.1|1536700_1536922_-|translation-initiation-factor-IF-1
MAKQPSIEQDGTITEALSNAMFRVELENGHVITAHISGKMRMHYIKILPGDKVKVEMSPYDLTKGRITFRYKN

Crispr_ID: NZ_AP018042_5

• CRISPR_ID: NZ_AP018042_5
• CRISPR_location: 2300886-2301074
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 4 spacers
• Cas_protein_info: csa3

Consensus repeat of NZ_AP018042_5

Consensus_repeat	Method
TTATTATTTGTTAATGGCTCCAC	CRISPRCasFinder
TTATTATTTGTTAATGGCTCCAC	CRT

spacers of NZ_AP018042_5

>5.1|2300909|19|NZ_AP018042|CRISPRCasFinder,CRT
CAAGGGCTTAGGCAAGGCA
>5.2|2300951|18|NZ_AP018042|CRISPRCasFinder,CRT
TAAATGATAATACGTTCA
>5.3|2300992|19|NZ_AP018042|CRISPRCasFinder,CRT
TAAAGGCTTAGGCTAGCCA
>5.4|2301034|18|NZ_AP018042|CRISPRCasFinder,CRT
CAAATGAAAAAACGTCCA

CRISPR arrays and Neighbor proteins around NZ_AP018042_5

CRISPR arrays

The CRISPR arrays of NZ_AP018042_5

>merge|NZ_AP018042|5|2300886-2301074|CRISPRCasFinder,CRT
TTATTATTTGTTAATGGCTCCACCAAGGGCTTAGGCAAGGCATTATTATTTGTTAAAGGCTCCACTAAATGATAATACGTTCATTATTATTTGTTAATGACTCCACTAAAGGCTTAGGCTAGCCATTATTATTTGTTAATGGCTCCACCAAATGAAAAAACGTCCATTATCATTTTATAATAGCTTCTC

>NZ_AP018042|5|4|2300886-2301074|CRISPRCasFinder
TTATTATTTGTTAATGGCTCCAC	CAAGGGCTTAGGCAAGGCA
TTATTATTTGTTAAAGGCTCCAC	TAAATGATAATACGTTCA
TTATTATTTGTTAATGACTCCAC	TAAAGGCTTAGGCTAGCCA
TTATTATTTGTTAATGGCTCCAC	CAAATGAAAAAACGTCCA
TTATCATTTTATAATAGCTTCTC

>NZ_AP018042|5|2|2300886-2301074|CRT
TTATTATTTGTTAATGGCTCCAC	CAAGGGCTTAGGCAAGGCA
TTATTATTTGTTAAAGGCTCCAC	TAAATGATAATACGTTCA
TTATTATTTGTTAATGACTCCAC	TAAAGGCTTAGGCTAGCCA
TTATTATTTGTTAATGGCTCCAC	CAAATGAAAAAACGTCCA
TTATCATTTTATAATAGCTTCTC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096433548.1|2296418_2296856_+|ribonuclease-P-protein-component	unknown	unknown	gnl|CDD|234989
NZ_AP018042.1|WP_096429016.1|2301227_2304002_-|DNA-polymerase-I	unknown	unknown	gnl|CDD|235591
NZ_AP018042.1|WP_096429010.1|2294638_2295547_+|DUF4271-domain-containing-protein	unknown	unknown	gnl|CDD|379465
NZ_AP018042.1|WP_096429022.1|2309236_2309572_-|nucleotide-pyrophosphohydrolase	unknown	unknown	gnl|CDD|212138
NZ_AP018042.1|WP_096429017.1|2304158_2304605_-|6-carboxytetrahydropterin-synthase	unknown	unknown	gnl|CDD|376501
NZ_AP018042.1|WP_096429020.1|2306151_2308056_-|PAS-domain-S-box-protein	unknown	unknown	gnl|CDD|223715
NZ_AP018042.1|WP_096429021.1|2308154_2309054_-|deoxyribose-phosphate-aldolase	unknown	unknown	gnl|CDD|188646
NZ_AP018042.1|WP_096429023.1|2309575_2310028_-|D-tyrosyl-tRNA(Tyr)-deacylase	unknown	unknown	gnl|CDD|376833
NZ_AP018042.1|WP_096429018.1|2304655_2304955_-|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	gnl|CDD|223713
NZ_AP018042.1|WP_096429015.1|2300092_2300488_+|MotA/TolQ/ExbB-proton-channel-family-protein	unknown	unknown	gnl|CDD|376576
NZ_AP018042.1|WP_096429019.1|2305159_2306146_+|polyprenyl-synthetase-family-protein	unknown	unknown	gnl|CDD|223220
NZ_AP018042.1|WP_096429007.1|2291994_2293047_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096429012.1|2296794_2297037_+|membrane-protein-insertion-efficiency-factor-YidD	unknown	unknown	gnl|CDD|376620
NZ_AP018042.1|WP_162845417.1|2293714_2294530_+|5'-nucleotidase,-lipoprotein-e(P4)-family	unknown	unknown	gnl|CDD|319836
NZ_AP018042.1|WP_096429011.1|2295583_2296336_+|uroporphyrinogen-III-synthase	unknown	unknown	gnl|CDD|119440
NZ_AP018042.1|WP_096429024.1|2310210_2311194_+|lipoate--protein-ligase	unknown	unknown	gnl|CDD|161920
NZ_AP018042.1|WP_096429025.1|2311264_2313067_-|excinuclease-ABC-subunit-UvrC	unknown	unknown	gnl|CDD|234792
NZ_AP018042.1|WP_096429008.1|2293058_2293544_-|RNA-polymerase-sigma-factor	unknown	unknown	gnl|CDD|224511
NZ_AP018042.1|WP_096429013.1|2297322_2299008_+|PDZ-domain-containing-protein	unknown	unknown	gnl|CDD|223864
NZ_AP018042.1|WP_096429014.1|2299104_2299872_-|LytTR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|197918

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_AP018042.1|WP_096429018.1|2304655_2304955_-|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	2.2e-16	55.1	0.4

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096433548.1|2296418_2296856_+|ribonuclease-P-protein-component	gnl|CDD|234989	PRK01903, rnpA, ribonuclease P protein component.	2.7739e-18
NZ_AP018042.1|WP_096429016.1|2301227_2304002_-|DNA-polymerase-I	gnl|CDD|235591	PRK05755, PRK05755, DNA polymerase I; Provisional.	0
NZ_AP018042.1|WP_096429010.1|2294638_2295547_+|DUF4271-domain-containing-protein	gnl|CDD|379465	pfam14093, DUF4271, Domain of unknown function (DUF4271). This family of integral membrane proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 221 and 326 amino acids in length.	2.72566e-25
NZ_AP018042.1|WP_096429022.1|2309236_2309572_-|nucleotide-pyrophosphohydrolase	gnl|CDD|212138	cd11531, NTP-PPase_BsYpjD, Nucleoside Triphosphate Pyrophosphohydrolase (EC 3.6.1.8) MazG-like domain putative pyrophosphatase YpjD from Bacillus subtilis and its bacterial homologs. This family includes a putative pyrophosphatase Ypjd from Bacillus subtilis (BsYpjD) and its homologs. Although its biological role has not been described in detail, BsYpjD shows significant sequence similarity to the dimeric 2-deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTP pyrophosphatase or dUTPase) and NTP-PPase MazG proteins. However, unlike typical tandem-domain MazG proteins, BsYpjD contains a single MazG-like domain.	9.17352e-42
NZ_AP018042.1|WP_096429017.1|2304158_2304605_-|6-carboxytetrahydropterin-synthase	gnl|CDD|376501	pfam01242, PTPS, 6-pyruvoyl tetrahydropterin synthase. 6-Pyruvoyl tetrahydrobiopterin synthase catalyzes the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. The functional enzyme is a hexamer of identical subunits.	3.55178e-32
NZ_AP018042.1|WP_096429020.1|2306151_2308056_-|PAS-domain-S-box-protein	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	2.5809e-36
NZ_AP018042.1|WP_096429021.1|2308154_2309054_-|deoxyribose-phosphate-aldolase	gnl|CDD|188646	cd00959, DeoC, 2-deoxyribose-5-phosphate aldolase (DERA) of the DeoC family. 2-deoxyribose-5-phosphate aldolase (DERA) of the DeoC family. DERA belongs to the class I aldolases and catalyzes a reversible aldol reaction between acetaldehyde and glyceraldehyde 3-phosphate to generate 2-deoxyribose 5-phosphate. DERA is unique in catalyzing the aldol reaction between two aldehydes, and its broad substrate specificity confers considerable utility as a biocatalyst, offering an environmentally benign alternative to chiral transition metal catalysis of the asymmetric aldol reaction.	1.20076e-71
NZ_AP018042.1|WP_096429023.1|2309575_2310028_-|D-tyrosyl-tRNA(Tyr)-deacylase	gnl|CDD|376833	pfam02580, Tyr_Deacylase, D-Tyr-tRNA(Tyr) deacylase. This family comprises of several D-Tyr-tRNA(Tyr) deacylase proteins. Cell growth inhibition by several d-amino acids can be explained by an in vivo production of d-aminoacyl-tRNA molecules. Escherichia coli and yeast cells express an enzyme, d-Tyr-tRNA(Tyr) deacylase, capable of recycling such d-aminoacyl-tRNA molecules into free tRNA and d-amino acid. Accordingly, upon inactivation of the genes of the above deacylases, the toxicity of d-amino acids increases. Orthologues of the deacylase are found in many cells.	5.882e-91
NZ_AP018042.1|WP_096429018.1|2304655_2304955_-|helix-turn-helix-transcriptional-regulator	gnl|CDD|223713	COG0640, ArsR, Predicted transcriptional regulators [Transcription].	1.1812e-14
NZ_AP018042.1|WP_096429015.1|2300092_2300488_+|MotA/TolQ/ExbB-proton-channel-family-protein	gnl|CDD|376576	pfam01618, MotA_ExbB, MotA/TolQ/ExbB proton channel family. This family groups together integral membrane proteins that appear to be involved translocation of proteins across a membrane. These proteins are probably proton channels. MotA is an essential component of the flageller motor that uses a proton gradient to generate rotational motion in the flageller. ExbB is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane.	1.08393e-06
NZ_AP018042.1|WP_096429019.1|2305159_2306146_+|polyprenyl-synthetase-family-protein	gnl|CDD|223220	COG0142, IspA, Geranylgeranyl pyrophosphate synthase [Coenzyme metabolism].	2.69052e-82
NZ_AP018042.1|WP_096429012.1|2296794_2297037_+|membrane-protein-insertion-efficiency-factor-YidD	gnl|CDD|376620	pfam01809, Haemolytic, Haemolytic domain. This domain has haemolytic activity. It is found in short (73-103 amino acid) proteins and contains three conserved cysteine residues.	2.02165e-38
NZ_AP018042.1|WP_162845417.1|2293714_2294530_+|5'-nucleotidase,-lipoprotein-e(P4)-family	gnl|CDD|319836	cd07534, HAD_CAP, molecular class C acid phosphatases, similar to Haemophilus influenzae e (P4) acid phosphatase; belongs to the haloacid dehalogenase-like hydrolase superfamily. Molecular class C acid phosphatases (CAPs) are nonspecific acid phosphatases with generally broad substrate specificity and optimum activity at neutral to acidic pH. Members include Haemophilus influenzae lipoprotein e (P4), Elizabethkingia meningosepticum OlpA, Helicobacter pylori HppA, Enterobacter sp. 4 acid phosphatase PhoI, and Streptococcus pyogenes M1 GAS LppC. Lipoprotein e (P4) exhibits phosphomonoesterase activity with aryl phosphate substrates including nicotinamide mononucleotide (NMN), tyrosine phosphate, phenyl phosphate, p-nitrophenyl phosphate, and 4-methylumbelliferyl phosphate. The role of P4 in NAD+ uptake appears to be the dephosphorylation of NMN to nicotinamide riboside, which is then taken up by the organism. Elizabethkingia meningosepticum OlpA is a broad-spectrum nucleotidase with preference for 5'-nucleotides, it efficiently hydrolyzes nucleotide monophosphates, with a strong preference for 5'-nucleotides and for 3'-AMP; OlpA can also hydrolyze sugar phosphates and beta-glycerol phosphate, although with a lower efficiency. Helicobacter pylori HppA is also a 5' nucleotidase. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	1.97314e-77
NZ_AP018042.1|WP_096429011.1|2295583_2296336_+|uroporphyrinogen-III-synthase	gnl|CDD|119440	cd06578, HemD, Uroporphyrinogen-III synthase (HemD) catalyzes the asymmetrical cyclization of tetrapyrrole (linear) to uroporphyrinogen-III, the fourth step in the biosynthesis of heme. This ubiquitous enzyme is present in eukaryotes, bacteria and archaea. Mutations in the human uroporphyrinogen-III synthase gene cause congenital erythropoietic porphyria, a recessive inborn error of metabolism also known as Gunther disease.	2.51523e-39
NZ_AP018042.1|WP_096429024.1|2310210_2311194_+|lipoate--protein-ligase	gnl|CDD|161920	TIGR00545, Probable_lipoate-protein_ligase_A, lipoyltransferase and lipoate-protein ligase. One member of this group of proteins is bovine lipoyltransferase, which transfers the lipoyl group from lipoyl-AMP to the specific Lys of lipoate-dependent enzymes. However, it does not first activate lipoic acid with ATP to create lipoyl-AMP and pyrophosphate. Another member of this group, lipoate-protein ligase A from E. coli, catalyzes both the activation and the transfer of lipoate. Homology between the two is full-length, except for the bovine mitochondrial targeting signal, but is strongest toward the N-terminus. [Protein fate, Protein modification and repair].	2.59219e-79
NZ_AP018042.1|WP_096429025.1|2311264_2313067_-|excinuclease-ABC-subunit-UvrC	gnl|CDD|234792	PRK00558, uvrC, excinuclease ABC subunit UvrC.	0
NZ_AP018042.1|WP_096429008.1|2293058_2293544_-|RNA-polymerase-sigma-factor	gnl|CDD|224511	COG1595, RpoE, DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog [Transcription].	1.27926e-32
NZ_AP018042.1|WP_096429013.1|2297322_2299008_+|PDZ-domain-containing-protein	gnl|CDD|223864	COG0793, Prc, Periplasmic protease [Cell envelope biogenesis, outer membrane].	5.1028e-91
NZ_AP018042.1|WP_096429014.1|2299104_2299872_-|LytTR-family-transcriptional-regulator	gnl|CDD|197918	smart00850, LytTR, LytTr DNA-binding domain. This domain is found in a variety of bacterial transcriptional regulators. The domain binds to a specific DNA sequence pattern.	1.00161e-18

Protein sequences

>NZ_AP018042.1|WP_096429015.1|2300092_2300488_+|MotA/TolQ/ExbB-proton-channel-family-protein
MKDMFFMGGPIFMGILSLLLIAMVSWIAYHFIGVLFSKNTILEQTLRKVGYGKSIGLFALIIGILGQLLGLFNAFSVIEQAGNISPSVIYGGIKVSMIPTFYGIIIYLLSILLWFVASILIEKKLEKQAIL
>NZ_AP018042.1|WP_096429014.1|2299104_2299872_-|LytTR-family-transcriptional-regulator
MIKKSSNPYYHIFYWIFVLLILTLVFGLSWGNNVAAFFFISMLLPIVLGTSYFFNYFLVPRYYMQKKYVRFGLYSFYTAVVSMYLETIVLMFSFIYLGNFNFGNLGPNASNTFLLAALLYLMVFIGSFLLMSRQIREKQQVIQEFLFEKEKLKQAYLEIISNRKTIKIKFSDIKYIESLADYVHIHTSTEKIKTKEKISSLEDILPDSFLRIHRSFIINKDWLKSFSYDHILVDDIQLNIGRTYRKQVKESLVVQ
>NZ_AP018042.1|WP_096429013.1|2297322_2299008_+|PDZ-domain-containing-protein
MKLRKKSGAIWLLAVLLFCSAAFWGFSRDERNFEISKNLNIYYTLFRELNLFYVDEIDPGDLVKKSMDAMLTTLDPYTTYISEAEIEDFKLMTTGEYAGIGSLISKHKDEVIIAEPYEGFPAFKAGLKAGDVLLEINGISVLKKTTDDVSSLLKGQPNVPLTIKIKRPGTDKTMEKKLVRENIQLKAVPYYGMVSDSIGYILLNQFTDKAAGEIKVALKDLKEQNAKSIVLDLRSNPGGLLDQAIEIVNLFVEKGEAIVNTKGKVSQWDKSYKATKTPFDAQIPIAVLVNRGSASASEIVSGAIQDLDRGVVIGQRTFGKGLVQTTRNLTYNAKLKVTTAKYYIPSGRCIQALDYSNRNEDGSVGKIADSLITEFHTKNGRSVFDGGGVLPDLKIESERYSSLTINLIRNFMVFDYATLYANTNDNISGSKEFKISDQEFDKFTKFIKEQKFKYESESTEILDALKKAAKEEKYFDISEDEFEALALKLTPDLDRDLTRFKDEISDLLAHEIIKRYYYQKGGIEYSLQEDPTLLKAIEVLENKTEYLGILDGSIGLHALNK
>NZ_AP018042.1|WP_096429012.1|2296794_2297037_+|membrane-protein-insertion-efficiency-factor-YidD
MKSILNFLKKTILFIMILPIRFYQYVISPLTPSACRYTPTCSSYAIQALKKHGPFKGSYLAIKRIFSCNPWGGHGHDPVP
>NZ_AP018042.1|WP_096433548.1|2296418_2296856_+|ribonuclease-P-protein-component
MNRFTFKKAERLTHKILIGKLFSEGKGFICYPFRIVWKEANLNSEYPAQVAITVSKRSFKKAVTRNLLKRRIREIYRLNKTEFYSELEIINVRLAFMIVYLPKTILKTSEMEDKLIKALKRLPKEYEKHSQLSEKDDSVHHDTSN
>NZ_AP018042.1|WP_096429011.1|2295583_2296336_+|uroporphyrinogen-III-synthase
MKIKTILVSQPKPQTEKSPYFDLAEKYNLKIDFRPFIQVEGISAKEFRQERINVLDHTAVIFTNRTAIDHFFRICQELRITVPDDMKYFCVSESTAFYLQKYIVYRKRKIFFGEKRFEDLIEIILKHKGETYLVPLSDIHKQSIPELLTKNKIKYSKAILYRTVSSDLSDLAHVNYDVLAFFSPSGIASLLQNFPEFKQNNTLIAAFGPSTSEAVTLNNLRLDILAPMPQAPSMTMALDQFIKKYNKENK
>NZ_AP018042.1|WP_096429010.1|2294638_2295547_+|DUF4271-domain-containing-protein
MAQFNSHDALVNKTPLHDSLIISGNEFKDSSTLQITKQITVNKDTRAFISPLIPTWSDCILFPDSIPAPIKNSTVQVIRGEKGIKIASYNSDWMAGVLLFALILLAVLRFSFSKFLFRVFDSMINYQTSSNLFLEKNMRNLRGSIFLNLLFYVNLALFIVQYFVYILSLNKTHNNIVFFLYCFAGLVTLYSSKLIFIRFIGYVFNGIKESKEYLHTVSIYNKNLGVILLPITLSVPYMAQHATPLLLNGGLILTLIFYVFRLIRGVKILFRQHVSIFYMILYLCALEILPLLMIYKLLYLLV
>NZ_AP018042.1|WP_162845417.1|2293714_2294530_+|5'-nucleotidase,-lipoprotein-e(P4)-family
MRKIFFALSAFSILISSCSQNLDSKKQKNNPNEHLVMATLWFQRASECRALYYQAFNMAKLSLDKQLSADSDKSKPKAVVVDIDETVLDNSPFETNSIRTGKSLTSKAWKEWTDMAKASPTPGSLEFLKYAESKGVETFYISNRDTSALLKTIENLKNLGFPFADKNHVLLKSETGIKTIRRNKVLETHDILILAGDNLGDFDEFLQDRSTDFGFSKVDQMKAQFGERFIVLPNPMYGSWEKEVMKSDEKLNPSEKENLRKKQLIGYEELK
>NZ_AP018042.1|WP_096429008.1|2293058_2293544_-|RNA-polymerase-sigma-factor
MNLNEYNISVDQHADGMFRFILKNIRDEDKARDIVQDSFEKLWRNVDNVNFMKVKSYLYTTAYHTMIDLIRREKRKEDFENVDVMDYSHSEHYSDLSEILDEALKRLSDVQRSVILLRDYEGYSYTEIAKITDLTESQVKVYIYRARKHLKNYIGSIESII
>NZ_AP018042.1|WP_096429007.1|2291994_2293047_-|hypothetical-protein
MKEITRHNYEEFFLDYLEGTLSSLQGIVLEDFLTKNPDLKAELEEMECPVLSKDTILADSESLKEIPFRESFDDFCIARLEGDLLVGDESAFDAFLDKNESFKKESEVYNKTILLADTNLVFRDKNKLKRSSRKVAFWWYFSRVGVAALIILVFSLWSILYQFKGDYSNEEKITAKVETVITERKEIPEGSLPVGVLIKPEQKVVQTEAKTSLKDGQKEIIDTPVNEAKIQDLDEVVERITPLDISLSTEFVAIANSANPAMEIDIPKDKKTEVNLQNNGLAQLGMSWKSSIPEKKAQTSVLFAIAKYGVDKLSEIAGKDVQFEQKYDSKTEKTRLDFNTAGIGFSKTIK
>NZ_AP018042.1|WP_096429016.1|2301227_2304002_-|DNA-polymerase-I
MHSSSTNSGKKLFLLDAFALIYRSYYAFIKNPRFTSTGINSSAMLGFTNTLVEVLEKEKPSHIAVVFDPVGPTFRHEMFKEYKAQRPPMPEDLRKSIPYIKSIIQGFNIPEFDVAGFEADDVIGTLAKKAEKEGFTVYMMTPDKDYAQLVSDKIFMYKPKSNGNDVEIWGVPEIKAKFGVEHPDQIVDYLGLMGDVSDNIPGCPGVGPKSAQKLLELYKSIDGLYENTHELKGKQKERIIESEEQVRFSKVLAKISLDVPIDFDEENFKLIDYDEVKLRAIFKELELFTLAERVFKNQGNLFSAESGESAGKDTVPDKDVILRDLKGLACDFFVLDNQASRADLKAELCVQEQFSFRTIISDSDPNTSNLLGLAFSYQKDKSFFVPFPQNFEDAKKVAQEFKFIFEDENILKIAHNCKRDILILRWHGVDVKGPLFDTMIAHYLIQPEFKHEFAFVAHTILDYKVIETEDKTKGKKAQLSLMLEPEVVTHDPFCEETVLNLELKEVLESRMKENNLLDLFYNIEMPLLSILADMEFTGVSIDVPTLNDYALVLNEEVDGIEKEIKDLAGVDFNVASPKQLGEVLFEKMELDSKAKKTKSGQFSTSEAVLSKLKGKHEIIQKILDFRSLKKLISTYVEALPTYVNEKSGKIHTSFNQAEAATGRLSSTNPNLQNIPIRTEQGRYIRKAFVPSSENHTFLSADYSQVELRLMAHMSQDQNMIDAFINSEDFHAATAAKIYKLPIEEVSKEMRSRAKTANFGIIYGISAWGLAERLDMSRTEGKELITGYFESYPGVKLFMDQCVEKAREDEFVTTLLSRRRYLKDINSSNAIVRSVAERNAINAPIQGSAADVIKIAMINIAKRMKLEGMQSKMIIQVHDELNFDCLLSELDQMKAILKEEMENAIQLSVPLTVDMGVGENWLQAH
>NZ_AP018042.1|WP_096429017.1|2304158_2304605_-|6-carboxytetrahydropterin-synthase
MTKVRLTKEFCFEMAHALWNYDGLCKNIHGHSYILFVTVIGEPITDANNSKLGMVMDFGDLKKIVSEEVVDKLDHSLVLSKATPNLEKLNIEQMFDRYFITDYQPTCENMIVDFAEKIGRRLPVDVKLHSLKLHETATSFAEWYAEDN
>NZ_AP018042.1|WP_096429018.1|2304655_2304955_-|helix-turn-helix-transcriptional-regulator
MKIRELEPEKLEQAANMLKAIAHPMRIAILGYLEDGKKLTVTEIHELLKIEQSTTSHHLGILKDKGVLSSKREGKNTYYYLKHCSLSNIVDCISNCTQG
>NZ_AP018042.1|WP_096429019.1|2305159_2306146_+|polyprenyl-synthetase-family-protein
MNLRKITIKDIKSPVKKEMEQFESFFKDSMKSKIRLLDIINRYIIKRKGKEIRPILVFLSAKLTGEINDSTYTAAALTQLLHTATLIHDDIVDEAYERRGFFSINALWKTKAAVLVGDFLLSKGLLIALDNNEFEQLKVVSNAVREMSEGELLQLEKSRKLNITEEVYFDIIYKKTASLMASCTQLGAISAKANYEDQQKLKKFGEYLGIAFQMKDDLFDYEKQGSIGKPTGNDIKEKKLTLPLIYVLREVSEKERRWALKIVRKHNKNSEKVNELIAFVKEKGGIEYTRDKMLEYKQKALDVLNAFPSSEAKEALVNIVDYTISRNK
>NZ_AP018042.1|WP_096429020.1|2306151_2308056_-|PAS-domain-S-box-protein
MYNESHDPLKNKVQELENEIKRLRKLIPQEYLTDEYFQSKSLNSKDSLSVIDTNFTLIDVNAKRLIGTGLKRSELIGEKCHKIFYGLDKPCDECVVPQIIKSKEANSFLKSNLDNNKIRFEEKHLSPVLNKKGNVERIIVQSKDISSYYHLVDRIGQREEFYKNIFESAGDAFLVHDETGRLIEFSSRLSDLLEYTKEELSNLNVSDIDDPSYSMHFDQRQKYIETEGSALFETSLISKSGERIPVEVSANLISFSNTNISFVAIRDIRKRKLAEKKLLESEERFRTLVENAPDLIMRFDVDYKHLFVNSASFNILKMQPEEFIGKTHFEMGFPKDKCEFWEREIDLVFQLKKPHSADFSISFEGEVHHFEWQLIPELNLAGECETILAIARDITVRKRSENAFKEAIKTKDKFFSIIAHDLKNPFNALLPIAKNLYQNCREMSEDQIFEASTIIHSAAKQEYNLLKNLLEWGRSQMGKIVNAPKQINLLELVESNIQLHKTKADRKNIHVRISKGANTMAFADEYMLDTVIRNLYSNAIKFTNPGGNIQIRIGRSGSKSIISVEDDGLGISEANQKRLFRLDTNYTRVGTCEESGTGLGLLLCYEFVIQNNGTISLESSSGKGSIFTVELPSN
>NZ_AP018042.1|WP_096429021.1|2308154_2309054_-|deoxyribose-phosphate-aldolase
MKKDILEILRSYDLKISDAEVKANVKSILDKDFEALYTIENLKKNFSFIDLTTLNSTDTHAKAKSFADNVNNFQNDFDMPNVAAICVYPYQVPTLNENLKAEGVRIASVAGVFPSSQSYVEVKALECKMAVEKGADDIDIVISIGAFLEGHYQTVFDEIVIQKEACGKAHLKVILETGELKTSENIRTASIIAMEAGADFIKTSTGKVPVNATLEAAYVMTQAIKEYFDRTGKMIGFKPAGGISTGKDAIEFFAISKSNLDENWLTNKYLRIGASSLANNLLKEIHFIETGEQKEIKYF
>NZ_AP018042.1|WP_096429022.1|2309236_2309572_-|nucleotide-pyrophosphohydrolase
MNQIGIKEAQEKVDWWIKKYGVRYFNELTNMAILTEEVGEVARIISRKYGEQSFKESDKAVDLADEMADVLWVLMCLANQTGVDLNEAFQKNIAKKTERDKDRHKGNDKLK
>NZ_AP018042.1|WP_096429023.1|2309575_2310028_-|D-tyrosyl-tRNA(Tyr)-deacylase
MRIVIQRVTQATVSVDSKIIGQISKGLMVLVGIETADTEEDSEWLSKKICNLRIFDDEEGVMNKSLMDVDGNILAISQFTLHAKTKKGNRPSYILAAKPDISVPLYENFVACLEKESGKKVETGEFGAEMQVSLVNDGPVTIVMDTKNKE
>NZ_AP018042.1|WP_096429024.1|2310210_2311194_+|lipoate--protein-ligase
MLYFISSSTNPSFNIATEEYLLKNYEDDFFFLYTNTPALIVGKHQNTLAEINLEKAEQENIPVVRRMSGGGTVFHDEGNLNYCFIKNGEKGKLVDFQKYSQPIVDTLQALNVDAKFEGKSDLTIDGKKFSGNASHIYKNKVMQHGTMLFSSDLKQLNQLLKVNPLKFKDRGIRSIRSKVTNISEYLSDSMSLEKFGQLILEDLKTKFLDAKKFELSAIDIEKIEALMAEKYNNWEWNYGYSPNYIFEKLSNFPGGGILELSMKVEKGIIKNLELFGNCLVENQISHFKTKLIGKYHDKTTIRKTLKELDLNLFFHEITDKELLSALF
>NZ_AP018042.1|WP_096429025.1|2311264_2313067_-|excinuclease-ABC-subunit-UvrC
MIKNKNTDHLKSLVSALPEEPGVYQFFDESNTIIYVGKAKRLKRRVASYFNKVHDSAKTNIMVRKIVDIRHIVVESEADALLLENNLIKKYQPRYNVLLKDDKTFPWICIKKEEFPRVLVTRYLLKDGAEYFGPYTSVRMVRVLMDMISSLYQLRTCNLKLSEEGVRDQKFKLCLEFHMGNCKAPCEGKENQEEYNLKIAEIRNILKGNIGTVTSHLKEKMQELSVDFKFEEAHIIKEKLDLLSKYQSRSTIVNPSINNVDVYSIVDDENFAYVNFIKVVNGAIIQAHTIEMKKKLDESKEDLLLLAITDIRQKIFSTAKEILVPIELDLVIENVRFIVPQRGDKKKLLDLSERNVKYYRLEKLKKGDKSIKQSHADRIVETMRTDLRLKDAPVHIECFDNSNIQGTIPVASCVVFRNARPYKKDYRHFNIKTVEGANDFASMEEIIYRRYRRLLDENKSLPQLIVIDGGKGQLGAALNSLDKLELRGKISVIGIAKKLEEIYFPGDPFPLYLDKNSETLKTIQHLRNESHRFAITFHRQKRSKAFIVSELDKIEGIGPKSVQKLIQKFKSVENVRKASVAELETELGNAKALTVHKYFN

Crispr_ID: NZ_AP018042_6

• CRISPR_ID: NZ_AP018042_6
• CRISPR_location: 2925828-2926297
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 7 spacers

Consensus repeat of NZ_AP018042_6

Consensus_repeat	Method
GTTAAGGCAGTATTTTTGCTAAGATCTAA	CRISPRCasFinder

spacers of NZ_AP018042_6

>6.1|2925857|34|NZ_AP018042|CRISPRCasFinder
GGAGTTGATTTTGTTATTGTGACAAGATAAAATT
>6.2|2925920|34|NZ_AP018042|CRISPRCasFinder
TGTCTTGAGTTGATTTTCGGAGCAACCTAAAGTA
>6.3|2925983|34|NZ_AP018042|CRISPRCasFinder
AGTGTTGATTAGATTAGTAGAGCAATATAAAGTA
>6.4|2926046|34|NZ_AP018042|CRISPRCasFinder
AGCCGTAAGTTGGTTAGATATACAATATAATTCA
>6.5|2926109|34|NZ_AP018042|CRISPRCasFinder
TGAGCTGAGTTGGTTTTTAACACAAGAGAAACGA
>6.6|2926172|34|NZ_AP018042|CRISPRCasFinder
TGTGACGAATGGGTTATTACCACAAACTAATTCA
>6.7|2926235|34|NZ_AP018042|CRISPRCasFinder
TTGGGTGAGTTGATTCTGTAGGCAAGATAAAGAA

CRISPR arrays and Neighbor proteins around NZ_AP018042_6

CRISPR arrays

The CRISPR arrays of NZ_AP018042_6

>merge|NZ_AP018042|6|2925828-2926297|CRISPRCasFinder
GTTAAAGCCGTATTCTTGCTAAGATCTAAGGAGTTGATTTTGTTATTGTGACAAGATAAAATTGTTAAGGCAGTATTTTTGCTAAGATCTAATGTCTTGAGTTGATTTTCGGAGCAACCTAAAGTAGTTAAGGCAGTATTTTTGCTAAGATCTAAAGTGTTGATTAGATTAGTAGAGCAATATAAAGTATTTAAAGCCATATTTTGGCTCAGATCTAAAGCCGTAAGTTGGTTAGATATACAATATAATTCAGTTAAAGCTATATTTTTGCTAACATCTAATGAGCTGAGTTGGTTTTTAACACAAGAGAAACGAGTTAAAGCTGTATTATTGCTAACATCTAATGTGACGAATGGGTTATTACCACAAACTAATTCAGTTAAAGTTGTATTTTTGCTAATATCTAATTGGGTGAGTTGATTCTGTAGGCAAGATAAAGAAGTTAAAGCCGTATTTTGGCTAGTCTCTAA

>NZ_AP018042|6|5|2925828-2926297|CRISPRCasFinder
GTTAAAGCCGTATTCTTGCTAAGATCTAA	GGAGTTGATTTTGTTATTGTGACAAGATAAAATT
GTTAAGGCAGTATTTTTGCTAAGATCTAA	TGTCTTGAGTTGATTTTCGGAGCAACCTAAAGTA
GTTAAGGCAGTATTTTTGCTAAGATCTAA	AGTGTTGATTAGATTAGTAGAGCAATATAAAGTA
TTTAAAGCCATATTTTGGCTCAGATCTAA	AGCCGTAAGTTGGTTAGATATACAATATAATTCA
GTTAAAGCTATATTTTTGCTAACATCTAA	TGAGCTGAGTTGGTTTTTAACACAAGAGAAACGA
GTTAAAGCTGTATTATTGCTAACATCTAA	TGTGACGAATGGGTTATTACCACAAACTAATTCA
GTTAAAGTTGTATTTTTGCTAATATCTAA	TTGGGTGAGTTGATTCTGTAGGCAAGATAAAGAA
GTTAAAGCCGTATTTTGGCTAGTCTCTAA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096429436.1|2912063_2913617_+|lysine-5,6-aminomutase-subunit-alpha	unknown	unknown	gnl|CDD|378113
NZ_AP018042.1|WP_096429443.1|2919941_2921060_-|ATP-grasp-domain-containing-protein	unknown	unknown	gnl|CDD|237195
NZ_AP018042.1|WP_096429439.1|2915353_2916559_+|acetyl-CoA-C-acetyltransferase	unknown	unknown	gnl|CDD|180261
NZ_AP018042.1|WP_153244848.1|2929992_2930160_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096429448.1|2932064_2934485_-|S9-family-peptidase	unknown	unknown	gnl|CDD|224423
NZ_AP018042.1|WP_096433602.1|2938490_2939009_-|N-acetyltransferase	unknown	unknown	gnl|CDD|225695
NZ_AP018042.1|WP_096429447.1|2931346_2931928_-|HutD-family-protein	unknown	unknown	gnl|CDD|377578
NZ_AP018042.1|WP_096429450.1|2940869_2941703_+|universal-stress-protein	unknown	unknown	gnl|CDD|238182
NZ_AP018042.1|WP_096429437.1|2913769_2914558_+|cobalamin-B12-binding-domain-containing-protein	unknown	unknown	gnl|CDD|379858
NZ_AP018042.1|WP_096429446.1|2930402_2930879_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096429442.1|2918789_2919788_+|electron-transfer-flavoprotein-subunit-alpha/FixB-family-protein	unknown	unknown	gnl|CDD|224936
NZ_AP018042.1|WP_096429434.1|2909510_2910518_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096429451.1|2941786_2942290_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096429441.1|2917995_2918781_+|electron-transfer-flavoprotein-subunit-beta/FixA-family-protein	unknown	unknown	gnl|CDD|224997
NZ_AP018042.1|WP_096429452.1|2942312_2944214_+|amidophosphoribosyltransferase	unknown	unknown	gnl|CDD|223112
NZ_AP018042.1|WP_096429435.1|2910524_2912057_+|DNA-mismatch-repair-protein-MutS	unknown	unknown	gnl|CDD|213210
NZ_AP018042.1|WP_096429438.1|2914637_2915297_+|3-oxoacid-CoA-transferase-subunit-B	unknown	unknown	gnl|CDD|188219
NZ_AP018042.1|WP_096429449.1|2939109_2940441_-|tRNA-(N6-isopentenyl-adenosine(37)-C2)-methylthiotransferase-MiaB	unknown	unknown	gnl|CDD|237676
NZ_AP018042.1|WP_096429440.1|2916749_2917889_+|acyl-CoA-dehydrogenase	unknown	unknown	gnl|CDD|173847
NZ_AP018042.1|WP_096433600.1|2935017_2938263_-|PD40-domain-containing-protein	unknown	unknown	gnl|CDD|143478

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096429436.1|2912063_2913617_+|lysine-5,6-aminomutase-subunit-alpha	gnl|CDD|378113	pfam09043, Lys-AminoMut_A, D-Lysine 5,6-aminomutase TIM-barrel domain of alpha subunit. Members of his family are involved in the 1,2 rearrangement of the terminal amino group of DL-lysine and of L-beta-lysine, using adenosylcobalamin (AdoCbl) and pyridoxal-5'-phosphate as co-factors. The structure is predominantly a PLP-binding TIM barrel domain, with several additional alpha-helices and beta-strands at the N and C termini. These helices and strands form an intertwined accessory clamp structure that wraps around the sides of the TIM barrel and extends up toward the Ado ligand of the Cbl co-factor, providing most of the interactions observed between the protein and the Ado ligand of the Cbl, suggesting that its role is mainly in stabilizing AdoCbl in the precatalytic resting state. This is a TIM-barrel domain.	0
NZ_AP018042.1|WP_096429443.1|2919941_2921060_-|ATP-grasp-domain-containing-protein	gnl|CDD|237195	PRK12767, PRK12767, carbamoyl phosphate synthase-like protein; Provisional.	1.33867e-18
NZ_AP018042.1|WP_096429439.1|2915353_2916559_+|acetyl-CoA-C-acetyltransferase	gnl|CDD|180261	PRK05790, PRK05790, putative acyltransferase; Provisional.	0
NZ_AP018042.1|WP_096429448.1|2932064_2934485_-|S9-family-peptidase	gnl|CDD|224423	COG1506, DAP2, Dipeptidyl aminopeptidases/acylaminoacyl-peptidases [Amino acid transport and metabolism].	1.72208e-56
NZ_AP018042.1|WP_096433602.1|2938490_2939009_-|N-acetyltransferase	gnl|CDD|225695	COG3153, COG3153, Predicted acetyltransferase [General function prediction only].	1.70601e-60
NZ_AP018042.1|WP_096429447.1|2931346_2931928_-|HutD-family-protein	gnl|CDD|377578	pfam05962, HutD, HutD. HutD from Pseudomonas fluorescens SBW25 is a component of the histidine uptake and utilisation operon. HutD is operonic with the well characterized repressor protein HutC. Genetic analysis using transcriptional fusions (lacZ) and deletion mutants shows that hutD is necessary to maintain fitness in environments replete with histidine. Evidence outlined by Zhang & Rainey (2007) suggests that HutD functions as a governor that sets an upper bound on the level of hut operon transcription. The mechanistic basis is unknown, but in silico molecular docking studies based on the crystal structure of PA5104 (HutD from Pseudomonas aeruginosa) show that urocanate (the first breakdown product of histidine) docks with the active site of HutD.	9.65171e-32
NZ_AP018042.1|WP_096429450.1|2940869_2941703_+|universal-stress-protein	gnl|CDD|238182	cd00293, USP_Like, Usp: Universal stress protein family. The universal stress protein Usp is a small cytoplasmic bacterial protein whose expression is enhanced when the cell is exposed to stress agents. Usp enhances the rate of cell survival during prolonged exposure to such conditions, and may provide a general "stress endurance" activity. The crystal structure of Haemophilus influenzae Usp reveals an alpha/beta fold similar to that of the Methanococcus jannaschii MJ0577 protein, which binds ATP, athough Usp lacks ATP-binding activity.	1.88134e-24
NZ_AP018042.1|WP_096429437.1|2913769_2914558_+|cobalamin-B12-binding-domain-containing-protein	gnl|CDD|379858	pfam16554, OAM_dimer, dimerization domain of d-ornithine 4,5-aminomutase. This family is the short dimerization domain of the enzyme D-ornithine 4,5-aminomutase. It sits between the TIM-barrel pfam09043 and pfam02310. The enzyme is an alpha2-beta2-heterodimer that converts D-ornithine to 2,4-diaminopentanoic acid by way of radical propagation from an adenosylcobalamin to a pyridoxal 5'-phosphate cofactor.	5.0265e-36
NZ_AP018042.1|WP_096429442.1|2918789_2919788_+|electron-transfer-flavoprotein-subunit-alpha/FixB-family-protein	gnl|CDD|224936	COG2025, FixB, Electron transfer flavoprotein, alpha subunit [Energy production and conversion].	1.79042e-128
NZ_AP018042.1|WP_096429449.1|2939109_2940441_-|tRNA-(N6-isopentenyl-adenosine(37)-C2)-methylthiotransferase-MiaB	gnl|CDD|237676	PRK14329, PRK14329, (dimethylallyl)adenosine tRNA methylthiotransferase; Provisional.	0
NZ_AP018042.1|WP_096429441.1|2917995_2918781_+|electron-transfer-flavoprotein-subunit-beta/FixA-family-protein	gnl|CDD|224997	COG2086, FixA, Electron transfer flavoprotein, beta subunit [Energy production and conversion].	4.63446e-96
NZ_AP018042.1|WP_096429452.1|2942312_2944214_+|amidophosphoribosyltransferase	gnl|CDD|223112	COG0034, PurF, Glutamine phosphoribosylpyrophosphate amidotransferase [Nucleotide transport and metabolism].	1.25988e-28
NZ_AP018042.1|WP_096429435.1|2910524_2912057_+|DNA-mismatch-repair-protein-MutS	gnl|CDD|213210	cd03243, ABC_MutS_homologs, ATP-binding cassette domain of MutS homologs. The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family also possess a conserved ATPase activity that belongs to the ATP binding cassette (ABC) superfamily. MutS homologs (MSH) have been identified in most prokaryotic and all eukaryotic organisms examined. Prokaryotes have two homologs (MutS1 and MutS2), whereas seven MSH proteins (MSH1 to MSH7) have been identified in eukaryotes. The homodimer MutS1 and heterodimers MSH2-MSH3 and MSH2-MSH6 are primarily involved in mitotic mismatch repair, whereas MSH4-MSH5 is involved in resolution of Holliday junctions during meiosis. All members of the MutS family contain the highly conserved Walker A/B ATPase domain, and many share a common mechanism of action. MutS1, MSH2-MSH3, MSH2-MSH6, and MSH4-MSH5 dimerize to form sliding clamps, and recognition of specific DNA structures or lesions results in ADP/ATP exchange.	5.61626e-46
NZ_AP018042.1|WP_096429438.1|2914637_2915297_+|3-oxoacid-CoA-transferase-subunit-B	gnl|CDD|188219	TIGR02428, 3-oxoadipate_CoA-transferase_subunit_B, 3-oxoacid CoA-transferase, B subunit. Various members of this family are characterized as the B subunits of succinyl-CoA:3-ketoacid-CoA transferase (EC 2.8.3.5), beta-ketoadipate:succinyl-CoA transferase (EC 2.8.3.6), acetyl-CoA:acetoacetate CoA transferase (EC 2.8.3.8), and butyrate-acetoacetate CoA-transferase (EC 2.8.3.9). This represents a very distinct clade with strong sequence conservation within the larger family defined by pfam01144. The A subunit represents a different clade in pfam01144.	2.65347e-106
NZ_AP018042.1|WP_096429440.1|2916749_2917889_+|acyl-CoA-dehydrogenase	gnl|CDD|173847	cd01158, SCAD_SBCAD, Short chain acyl-CoA dehydrogenases and eukaryotic short/branched chain acyl-CoA dehydrogenases. Short chain acyl-CoA dehydrogenase (SCAD). SCAD is a mitochondrial beta-oxidation enzyme. It catalyzes the alpha,beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD, which becomes reduced. The reduced form of SCAD is reoxidized in the oxidative half-reaction by electron-transferring flavoprotein (ETF), from which the electrons are transferred to the mitochondrial respiratory chain coupled with ATP synthesis. This subgroup also contains the eukaryotic short/branched chain acyl-CoA dehydrogenase(SBCAD), the bacterial butyryl-CoA dehydorgenase(BCAD) and 2-methylbutyryl-CoA dehydrogenase, which is involved in isoleucine catabolism. These enzymes are homotetramers.	0
NZ_AP018042.1|WP_096433600.1|2935017_2938263_-|PD40-domain-containing-protein	gnl|CDD|143478	cd07562, Peptidase_S41_TRI, Tricorn protease; serine protease family S41. The tricorn protease (TRI), a member of the S41 peptidase family and named for its tricorn-like shape, exists only in some archaea and eubacteria. It has been shown to act as a carboxypeptidase, involved in the degradation of proteasomal products to preferentially yield di- and tripeptides, with subsequent and final degradations to free amino acid residues by tricorn interacting factors, F1, F2 and F3. Tricorn is a hexameric D3-symmetric protease of 720kD, and can self-associate further into a giant icosahedral capsid structure containing twenty copies of the complex. Each tricorn peptidase monomer consists of five structural domains: a six-bladed beta-propeller and a seven-bladed beta-propeller that limit access to the active site, the two domains (C1 and C2) that carry the active site residues, and a PDZ-like domain (proposed to be important for substrate recognition) between the C1 and C2 domains. The active site tetrad residues are distributed between the C1 and C2 domains, with serine and histidine on C1 and serine and glutamate on C2.	1.57555e-68

Protein sequences

>NZ_AP018042.1|WP_096429443.1|2919941_2921060_-|ATP-grasp-domain-containing-protein
MNVLLIDDKGSPYLLDTIRCLCNDKGMNIFVVSFERKPYFNTIPYSKHVNKYIYLTAIDEKDKVNKIIEFVKEWKIDTILPIKHRGYQLFSKYREEFNGSPLPPIPNRNDFETISDKWLLAQWLEKHSFASPKSYPVAIANIKKLNLPFLLKPRWDFKDNRIKLLHDINEAMEYIENTDFVSENFIFQEHIQGDDIDISLLAKDGEIIAYTIQQGLVREAFSFATGIEFVADAQLLAQTKAIIHDLNWSGIAHLDFIHQKESNSYYLIDFNPRMWSTLIGSLYAGVNFPLLMLKTAKNEKIEYKGYIETSFYLAKPALLHFRKELIHKRKLTSLKNSSWNYIIKDPLPEIMKGFGKVISIFNRSTKKEPPPA
>NZ_AP018042.1|WP_096429442.1|2918789_2919788_+|electron-transfer-flavoprotein-subunit-alpha/FixB-family-protein
MNLEDYKGIYVFIEQREGIIQNVALELLGQARKLADELNNKVYAMFLGDQITDQAQGLIACGADEVIVVDAPELKEYTTEPYTQAICQIINERKPESLFIGATTLGRDLGPRVSARIGTGLTADCTKIEIGENGEMLMTRPAFGGNLMATIVCKNHRPQMGTVRPGVLFAMDADKSRTGAIVNYQVKFNAAKFRVKLLETVKEDHNLIDITEAKILVSGGRGIGNKEGFEAMDALATTLHAEVSASRAMVDAGYIGHDRQVGQTGKTVRPDLYFAFGISGAIQHVAGMEDSDLIIAVNKDKNAPIFQVSDLGIVGDAKQIIKKLTERLEKQN
>NZ_AP018042.1|WP_096429441.1|2917995_2918781_+|electron-transfer-flavoprotein-subunit-beta/FixA-family-protein
MKIVVCIKQVPDTTEIKIDPVTGTLIRDGVPSIMNPDDKSGLEMALQLKDEKGAHVTVITMGPPQADLILREAYAMGVDRAIHLTDRKFAGADTLATSHALAGALRKIDFDLLITGRQAIDGDTAQVGPQIAEHLDLPQVSYLEDLTFDGDKTFTMKRHIEDGYQMLEVEAPCVVTVLSSANTPRYMNVGGIVDAYKNEVEVWGFNEIEVDEKDLGLKGSPTSVYKAFARGLKPSGELYEVGTDEAVGIIIKKLKEKFILN
>NZ_AP018042.1|WP_096429440.1|2916749_2917889_+|acyl-CoA-dehydrogenase
MNFSLTKEQELFLQMIKDFAEREVKPLAAEVDDLERFPIETVEKMAKIGIMGIPIPKQYGGAGGTNVMYSMAVEELSAVCATTGVIVSAHTSLCAAPILEYGTEAQKQKYLPKLASGEWIGAFGLTEPNAGTDASGQQTTAIEDGDNYIINGSKIFITNAGFAHVYVILAMTDKSQGTRGITAFIIEKGTPGFSIGKKEKKMGIRGSATCELVFENCLVPKENMLGKLSKGFGIAMKTLDGGRIGIAAQALGIAQGAIDETVKYVKERKQFGRPISAFQNTQFQLADMNTKTEASRMLVRKAAFKKDAKLPYSVDAAMAKLYAAETAMEVTNKAVQLHGGYGYTREYPVERMMRDAKITEIYEGTSEVQKMVISANMLK
>NZ_AP018042.1|WP_096429439.1|2915353_2916559_+|acetyl-CoA-C-acetyltransferase
MSKVYIVAAKRTAVGKFLGALTPVKAADLAAIVIKNIISETGIDAAKIDEVVIGNILMAGQGQGIARQASIKGGIPQEVPAYGINMICGSGMKTISLAYANIKAGEANLILAGGTENMSDAGFVMPGTVRGGHKMMDLKAVDHMVLDGLTDAFSGYHMGITAENIADKYNLTREEQDAFSFASQQKAMAAQDNGNFKNEIVPVEIKSRRETIIFDADEFINRGTSVEKLAGLRPAFKKDGTVTAGNASGINDGAAFVMVASEEAVKEFGLTPLAEIVATGQGGVDPAIMGMGPVPAIASALKKANMKLEQMDVLELNEAFAAQSLGVVKQLCEDHAVDANFFAERCNLNGGAIALGHPIGASGTRITVSLIHEMKRSGKEYGLASLCIGGGMGTAIILKNV
>NZ_AP018042.1|WP_096429438.1|2914637_2915297_+|3-oxoacid-CoA-transferase-subunit-B
MDKNEIREVIARRAALELHDGDVVNLGIGLPTIIPNYVPEDVNVILQSENGLLGMGAAPAEGEEDLDFVNAGGGFITCKKGASSFDSSVSFGIIRGGHVDVTVLGALQVDEKGNLANWIIPGKKTPGMGGAMDLIVGAKRVILAMEHTARGSHKIMTDCNLPLTAAGQVDMIITEMGVMDIVPEGILLKEINPLFTIEQIQEATDAKLIIAEDLIEMKK
>NZ_AP018042.1|WP_096429437.1|2913769_2914558_+|cobalamin-B12-binding-domain-containing-protein
MSGGLYSTNSNEFDKTLDLTKIKPYGDTMNDGKTQVSFTLPVEKGDEAIEAAKQMMRKMGFENPQVVYVQELMKGFTFFNCYGDCVHTVDFTGITVPKVEATAMDMHETDDFIKENIGRPIRILGASTGSDAHTVGIDAIMNMKGFAGHYGLERYGMMEALNMGSQVPNEEFIAKAIEFKADVLLVSQTVTQKDIHIKNLVELVELMEAEGLREKVILICGGPRISHELAKELGYDAGFGMNKYADDVASYAAQELSRRLNA
>NZ_AP018042.1|WP_096429436.1|2912063_2913617_+|lysine-5,6-aminomutase-subunit-alpha
MQRSKLGLDFEKVGHAKEVSKRIADDVQKFVENYSTVSVERTLCRLLGIDGVDKNEVPLPNVVIDELQEKGVLSEGVMFFLGNAILETGLNPQEIAEKVAVGELDITKIPVRSTEEIHKAIEPFVTKSIQVIRDRKAKRDNYINTIGEGPKPYLYVIVATGNIYEDVVQAEAAARQGADIIAVIRTTGQSLLDYVPYGATTEGFGGTVATQENFRIMRTHLDKIGEEEGKYIRLCNYCSGLCMPEIAAMGAIEGLDVMLNDALYGILFRDINMQRTIVDQYFSRLLNGFAGVIINTGEDNYLTTADAFDEAHTVLASDFINEQLALVAGLPEEQMGLGHAFEMEPGLENGFLYELGQAQMIREIFPKAPLKYMPPTKFMTGNVFKGHVQDALFNQISIWTGQGIQLLGMLTEAIHTPFMSDRYLSIENAKYIFNNMKSIGDEMEFKEGGIIRQRAAKVLDDAIELVEKIEKEGLFNALEKGIFADIKRPKDGGKGLAGVVEKGSNYFNPFIELLHKK
>NZ_AP018042.1|WP_096429435.1|2910524_2912057_+|DNA-mismatch-repair-protein-MutS
MQDLKSNVIETLRLNTDVEIDENSRQSDAVLSPISQPVLFREVAAHTSGLQFLMEDLDLRSPMGRRYLLDSKLMCCEKEISAELNFIDQLEQALELNSDLISKIQSKLSHLRDIRGSVNNLIGNLVLDDVELFEIKLFALLAQEIVELQKEGKQSFLEISDLSQLIRLLDPQNTKIPSFYIYDNYSEELAEARKELKLAKSKNGESDLDIEVLFAKMQEIEANVREELCTKISQFGGLLANALNQLAHLDLLIAKAVQAKKWDFSKAETSEGETSYTGMFNPMIKNELANQNKEYQAIDIDLERSVCLITGANMAGKTVVLKTLALCQYLFQFGFFVPATSAQISIVDKVMISVGDEQSELNGLSSFASEMLNVSRMVKDSKNQNNVLILIDELARTTNPVEGLAIVNAVADIFYQGKIRSVITTHYSGLKYKFRKLRVKGLDKDLGANVITYKNINSYMDYSLVEDLEGEVPHEALRIASLLGIDKEIIERAQNHLNEETSKENTFIKG
>NZ_AP018042.1|WP_096429434.1|2909510_2910518_+|hypothetical-protein
MPFLNDIENYKSLSIVGLEKNTGKTECLNYVLSRLRDSRKQIALTSIGIDGENRDQVTRTNKPEIELSEGMIFVTSELHYKQRKLVSEIIDVSRQSTSLGRLVTAKALTKGKVLLSGPASNHSIKELIADLKNKGVELTIVDGALSRKSIGSPAVTDAMILATGAALSANITQLVFKTKFVYDLIQLPEIEMNLKTQLSNVDRGIWAIDSEGTFQDTGISSVLLIKKEKDRLFQHGNRFFVSGAVTDQFLEFLKNQKQISEINLIVRDFTRVFASPETYYAFVKRGGSMQVLNKTNLLAITINPVSPDGYRLNSDELKRALQDKVNVPVYDIKKM
>NZ_AP018042.1|WP_153244848.1|2929992_2930160_-|hypothetical-protein
MTRGYFSHPKRPPRDLREPELDPGRCNLARRGTCRDLAGPLVDAKGYLFRKTLPN
>NZ_AP018042.1|WP_096429446.1|2930402_2930879_-|hypothetical-protein
MSADHKVNIQFTDEQKATIEAAIKSLKDTLGPVLMSLTPSEKQSMLILGDKSLSFVNKNLMFSKQNPEFMPSYLNQSEWEIDLRSWNALAPYNSQLAELGSLLNDTIAQCGNEAYRQSLTYYNNVRQAAKDNVPSAKPIYDDLKQQYPNHRKSKEVAD
>NZ_AP018042.1|WP_096429447.1|2931346_2931928_-|HutD-family-protein
MNFEISKAADFKTINWSGGTSTQLFIYPPTSDYQRRDFDFRLSTATVEVEESDFTSLPEVSRKIMILDGAIEIIHENRYRKKLRKFDTDSFEGGWKTSSVGKCIDFNLMTRGTASGEILALSIEKNKATTISIDGKADYLILYVFSGEISLTTNQEIHQLQQGNLLVITQFDSLNLKLSGSKDSEVILSEIIL
>NZ_AP018042.1|WP_096429448.1|2932064_2934485_-|S9-family-peptidase
MKRFIYLALLLGISSANAQENISYQKPSSEILDLVDVKVAPSVLIDESKDFMVLRYRDSYKTIAELSEIELRLGGLRINPKTNIGSRTNYYNDLKLINLKETNASPKPVTGLPENPRLANFKWSPDQKKIAFTHTSQTGVEIWVLDLKKAVVTKLTEAKINANMGDVINWFEDSKSLLVKMISEDKKELIDTENSIPTGPTISVNEGSKAQNRTYQDLLKNKNDEYNFEQLALSDLYKISLNGDKAKWLGAAMFGSISFSPDGKYVMLTTVEKPFSYLVPYSRFPFKTTIYSSKGEMVQTVLEVPLIEELPKGFMAIRTGRRNLNWRDDKPATLIYAEALDGGDPEIKVDFRDEVFELEAPFNGTPKSILKTINRFYTIDWANDNMAIAMDYWWNSRNTKAYVFNPSDASQKAKIISDRNYQDKYNDPGNFVHKRNKFGNYVLALEGDNAFLIGDGYSEKGQYPFVDQLNLKTEKTERLYQSTYEEKIEDIQDFDPVNKELLVRIESKTEYPNYYFRDIDKAKLKQLTSFENPFKSIQNVHKEVLSYKREDGLELSGTLYLPVGYDANKKEKMPMILWAYPEEFKDKASASQNTQNPNEFTYPYWASPIYWVTKGYVVLDDASFPIVGEGETEPNDSFRKQLVSNAKAAIDAVDALGYIDRNKVAVGGHSYGAFMVANLLSHSDLFAAGIARSGAYNRTLTPFGFQSEERSYWDSPEVYYKMSPFMHADKMKTPLLLIHGEADNNSGTYPMQSERYFNALKGLGANVRLVIFPKESHGYRAKESILHMLWEQDQWLDKYVKNRKAE
>NZ_AP018042.1|WP_096433600.1|2935017_2938263_-|PD40-domain-containing-protein
MKKLFFITTFLVALAQSLLAMDTPQWLRYPSISPNGQTIVFEYGGDLFTIPAEGGNATSLTSNQAYDYQPIWSPDSKTIAFASDRYGNFDIFTIPATGGAPNRITFNSKGETPSSFTPDGQNILFSSTISDDSRNAMFPSGVLPELYSVPAKGGKTSQVLTTPALDAKYSKDMNYILYRDSKGYENHWRKHHTSAVTRDICLYDVQNKTHSQFSTFKGEDLYPVFNSANDEIYYLSEKSGSFNVWKAPVSNKTQLTQISTFDKHPVRFLSISENDKLCYFWNGEIYTQEEGKDPIKLNVNITTDRKENEVSFEKLSSGATEMSVSSDGKQVAFIIRGEVFVTSVEYGTTKRITNTPEQERSVSFSPDDEALLYASERNGSWNLYQSKMTREDEAQFSNSTLLKEEAILEIPAETYQPKYSPDGKEIAFLENRVILKVINIESKKVRTILGKEWNYSYSDGDQHYDWSPDGKWFLVNIYPHTLFMPDAAMVDAQGNQKVINLTESGYSDSEAKWSLKGDAMIWFTDKRGYRSHGSWGSQSDVYVQFFNQKAFDDFKLSKEEKEIKEEAEKDKKEEDKDAEKDKKKDKKDKKEKDKVEDLNIEFSGMEDRQVCLTINPSNLSDAILTPDGKKLFYLSKFEDGYDLWVNDLEEKETKKILDLKGGGGAMQFDKEAKNLFLMSGKSIIKVDVATNKRKDISYTAEMYLDKAKEREYLFEHVWRTMLRKFYDPEMHQLDWDFYKSEYKRFLPYINNNYDFAEMLSELLGELNASHTGSGYRAKYKNADKTAELGAFLDWNFEGDGLRIAEVLEKGPLNQADSKIKAGTIIEKIDGNVIPKNESYFPLLNHKAGKKVLLSLYNPYTKERWEETVKPVSSINTLLYERWVKKRQAECERLSNGRIGYVHVKGMDSPSYRKVYSEMLGKYATYEAIVVDTRFNGGGWLHDDLATLLSGEVYSTLSPRGQDFGTEPMNKWKKPSAVLISESNYSDACAFPYVYQTLKIGKLIGMPVPGTMTAVWWETLQDQSLYFGMPMVGVKDTKGHYIENQQIEPDIKVANDYEVVTKGDDQQLKKAVEYLLQEIDGK
>NZ_AP018042.1|WP_096433602.1|2938490_2939009_-|N-acetyltransferase
MKIKIRPENKNDYKVISMINDMAFGQEAEGILVEKLRRNKKYSKDLSLVACMGNEIVGHILFFPVEIKADDNTFEALALAPMSVIPELQGLGIGSQLLENGLAKAKKLGHKSVIVLGHPEFYSKFGFTPASKFKITCPFEVTDDVFMALELEKDSLKSVSGCVAYPKEYKDL
>NZ_AP018042.1|WP_096429449.1|2939109_2940441_-|tRNA-(N6-isopentenyl-adenosine(37)-C2)-methylthiotransferase-MiaB
MSNNSKKLFLETYGCQMNVADSEVVASIMEHDGFTIIKTREEADVILVNTCSIRENAETRIRGRVQGFSELKKKNPKLLIGVIGCMAERLGEKLFDQEKNVNIIVGPDAYMDLPLLVKKAENGEKAINVELSLTETYKDICPSRIDETSISGFVSITRGCNNFCTYCIVPYTRGRERSRNPESILQEVNDLSEKGYKEVTLLGQNVNSFVFKNSISEVSFPELLEMVAISVPNMRIRFATSHPKDMSDDTLRVIAKNDNICNFIHLPVQSGSDSVLKDMRRKYTQEWYKNRIEAIHKIIPGCGISSDIFCGFHNESEEDFNQTIELMKWAKFDLAYMFKYSERPGTFAYKNLADNVPEDVKQRRLEEMIVLQNQISLENNQGDIGKVFEVLIEGISKRSEQDVYGRTSQNKVIVFPKKEYKIGDLANVIVKTCTKATLVGEAV
>NZ_AP018042.1|WP_096429450.1|2940869_2941703_+|universal-stress-protein
MKRILVPVDFSGDSLKALRFGIYLSNKLDCHLRMVHVQKSEKFEIPFHFEELKNEIIHTVQEYFEKLIKLHSADYCVRNGVFDFKIRTGSVYREIVNQAKYGDAYMILMGAYGASGFEEFFIGSNAVKVVTNSTCPVITVQKEFTKGQLKTIVMPIDASTDTRKKIPFVKEIASRCNAKVHILGVHETSDTNVIGKIEHYMKQGEDYLKDANIDYVKSLRKGDNNTYSTLDYAREVNADLIVIMTEQAESSINMFFGSYAQQMINRSEIPILSIPND
>NZ_AP018042.1|WP_096429451.1|2941786_2942290_+|hypothetical-protein
MQYVKIVILSFFILSGISSNAQDVQFGDVGKNDSLGKVNVIQDARILELLKTDEMINSRDPGFPGYRIQIFSGRSTDREKAFRVKKEFRELFPDERVYVVYKAPDFRVTVGNFRSKIESIELNKACLKFFPNSYPVKTYILFSDLTPIQQELDEVVLDEENEQQNAN
>NZ_AP018042.1|WP_096429452.1|2942312_2944214_+|amidophosphoribosyltransferase
MSDQLKHECGIAMIRLLKPLEYYQEKYGSWRYGIQKLYLLMEKQHNRGQEGAGAVGLKLDMPPGNTYIHRERSCSDQPIKDVFDGVYSELAAAEAKNPEKFNDPAWAKANLPFAGELYLGHLRYGTFGRNSIDFVHPVMRENNWKSRNLVLAGNFNLTNVDELFNLLVGLGQCPKNYTDTVTILEKVGHYLDEENQMLFRQYKNDGLSNQEISPQIERNIDIKQVLSNASRDWDGGYAIAGLFGHGDSFAMRDPWGIRPAFYYHDDEIVVVASERPVIQTALNVKADTIKEIDPGNALIIRKNGDVAEVPVRVAQKRRSCSFERIYFSRGSDKDIYKERKKLGQLLTPAILESVGHDMKNTVFSFIPNTAETSFYGMMEGVRHHLTEQKKQQIHDLNGNWTEEKLQEIISVEPRVEKIAIKDAKMRTFISNDEGRDDLVGHVYDVTYGIVKNKEDNLVVIDDSIVRGTTLKQSILRILDRLHPKKIVIVSSAPQIRYPDCYGIDMTRMGEFIAFNAAIAMLKEQGLESIIDETYKKCKAQENLPKEEVVNYVKEIYKPFTAEAISDKIAVLLTPEVMDAEVKIVYQTIENLHIACPDNTGDWYFTGNYPTPGGNKVVNTSFINYCEGNDKRSY

Crispr_ID: NZ_AP018042_7

• CRISPR_ID: NZ_AP018042_7
• CRISPR_location: 3856765-3857977
• CRISPR_type: TypeVI-B1,TypeVI-B2
• Repeat_type: NA
• Spacer_info: 18 spacers
• Cas_protein_info: cas13b,Csx27

Consensus repeat of NZ_AP018042_7

Consensus_repeat	Method
TTGTAANTGNTNTTATTTTGAAGGGTATACACAA	CRT
TTATTTTGAAGGGTATACACAAC	PILER-CR
TTATTTTGAAGGGTATACACAAC	CRISPRCasFinder

spacers of NZ_AP018042_7

>7.1|3856798|33|NZ_AP018042|CRT
ACGCTATATATCTGTATGTTGCAAAACATGTTG
>7.2|3856864|33|NZ_AP018042|CRT
ACACTAACTACACACAAAATAATTGCTAGAAAG
>7.3|3856930|33|NZ_AP018042|CRT
ACGTTGAATACATATACTGACTAAGTATTTTAG
>7.4|3856996|33|NZ_AP018042|CRT
ACTAAGTTGGGTTATTGTGTTGTTTAAGTTCTG
>7.5|3857062|33|NZ_AP018042|CRT
ACATGAAATAAGTTATTGTCACTTGAATTCCAG
>7.6|3857128|33|NZ_AP018042|CRT
ACTTATCAACGGGTTTGCCCGATAAACCTTTTG
>7.7|3857194|33|NZ_AP018042|CRT
ACTTTGATTTTTGCATAAAAGAAATAAAATCTG
>7.8|3857260|33|NZ_AP018042|CRT
ACACGTATAGCAAAGAGTTTTGTGACAAACATG
>7.9|3857326|33|NZ_AP018042|CRT
ACTTGTAGGTTAATACTCCATCAATGAAGAAAG
>7.10|3857392|31|NZ_AP018042|CRT
TTGTAATTTTTTAAATCTCTAATCATATCGG
>7.11|3857456|32|NZ_AP018042|CRT
ACATTCATTGCCCCGTGCTTTAGCTCGGGGTT
>7.12|3857521|33|NZ_AP018042|CRT
CAATTTTCAATGCTCATAATTTTAATTTTGAGT
>7.13|3857587|33|NZ_AP018042|CRT
CAGTTAATGATGCGTTCAAAGAGATTTTATTGT
>7.14|3857653|32|NZ_AP018042|CRT
CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
>7.15|3857718|32|NZ_AP018042|CRT
CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
>7.16|3857783|31|NZ_AP018042|CRT
CTCTTTCCCATGATTTGAAATTTCACATTGT
>7.17|3857847|32|NZ_AP018042|CRT
CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
>7.18|3857912|32|NZ_AP018042|CRT
TATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
>7.19|3856800|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
GCTATATATCTGTATGTTGCAAAACATGTTGTTGTAATTGCTC
>7.20|3856866|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
ACTAACTACACACAAAATAATTGCTAGAAAGTTGTAATTGCTC
>7.21|3856932|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
GTTGAATACATATACTGACTAAGTATTTTAGTTGTAATTGTCT
>7.22|3856998|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
TAAGTTGGGTTATTGTGTTGTTTAAGTTCTGTTGTAAATGCTC
>7.23|3857064|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
ATGAAATAAGTTATTGTCACTTGAATTCCAGTTGTAATTGCTC
>7.24|3857130|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
TTATCAACGGGTTTGCCCGATAAACCTTTTGTTGTACTTGCTC
>7.25|3857196|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
TTTGATTTTTGCATAAAAGAAATAAAATCTGGTGTAACTGCTC
>7.26|3857262|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
ACGTATAGCAAAGAGTTTTGTGACAAACATGTTGTAATTGCTT
>7.27|3857328|43|NZ_AP018042|PILER-CR,CRISPRCasFinder
TTGTAGGTTAATACTCCATCAATGAAGAAAGTTGTAGTTGCTC
>7.28|3857394|41|NZ_AP018042|PILER-CR,CRISPRCasFinder
GTAATTTTTTAAATCTCTAATCATATCGGTTGTAATTGCTC
>7.29|3857458|42|NZ_AP018042|PILER-CR
ATTCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTT
>7.30|3857523|43|NZ_AP018042|PILER-CR
AATTTTCAATGCTCATAATTTTAATTTTGAGTTGTACTTGCTC
>7.31|3857458|41|NZ_AP018042|CRISPRCasFinder
ATTCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTT
>7.32|3857522|43|NZ_AP018042|CRISPRCasFinder
AATTTTCAATGCTCATAATTTTAATTTTGAGTTGTACTTGCTC
>7.33|3857588|43|NZ_AP018042|CRISPRCasFinder
AGTTAATGATGCGTTCAAAGAGATTTTATTGTTGTAACTGCTC
>7.34|3857654|42|NZ_AP018042|CRISPRCasFinder
ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTT
>7.35|3857719|42|NZ_AP018042|CRISPRCasFinder
ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAGGTTT
>7.36|3857784|41|NZ_AP018042|CRISPRCasFinder
TCTTTCCCATGATTTGAAATTTCACATTGTTGCAATTGCTT
>7.37|3857848|42|NZ_AP018042|CRISPRCasFinder
ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAGAGTTT
>7.38|3857913|42|NZ_AP018042|CRISPRCasFinder
ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAATATTT

CRISPR arrays and Neighbor proteins around NZ_AP018042_7

CRISPR arrays

The CRISPR arrays of NZ_AP018042_7

>merge|NZ_AP018042|7|3856765-3857977|CRT,PILER-CR,CRISPRCasFinder
TTGTAATTGCTCTTATTTTGAAGGGTATACACAACGCTATATATCTGTATGTTGCAAAACATGTTGTTGTAATTGCTCTTATTTTGAAGGGTATACACAACACTAACTACACACAAAATAATTGCTAGAAAGTTGTAATTGCTCTTATTTTGGAGGGTATACACAACGTTGAATACATATACTGACTAAGTATTTTAGTTGTAATTGTCTTTATTTTGAAGGGTATACACAACTAAGTTGGGTTATTGTGTTGTTTAAGTTCTGTTGTAAATGCTCTTATTTTGAAGGGTATACACAACATGAAATAAGTTATTGTCACTTGAATTCCAGTTGTAATTGCTCTTATTTTGAAGAGTATGCACAACTTATCAACGGGTTTGCCCGATAAACCTTTTGTTGTACTTGCTCTTATTTTGAAGGGTACACACAACTTTGATTTTTGCATAAAAGAAATAAAATCTGGTGTAACTGCTCTTATTTTGAAGGGTATACACAACACGTATAGCAAAGAGTTTTGTGACAAACATGTTGTAATTGCTTTTATTTAGAAGGGTATACACAACTTGTAGGTTAATACTCCATCAATGAAGAAAGTTGTAGTTGCTCTTATTTTGAAGGGTATACACATTGTAATTTTTTAAATCTCTAATCATATCGGTTGTAATTGCTCTTATTTTGAAGGGTATAAACAACATTCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTTTGATTTTGAAGGGATACACAACAATTTTCAATGCTCATAATTTTAATTTTGAGTTGTACTTGCTCTTATTTAGAAGGGTATACACAACAGTTAATGATGCGTTCAAAGAGATTTTATTGTTGTAACTGCTCTTATTTTGAAGGGTATACACAACATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTTTGATTTTGAAGAGTATGCACAACATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAGGTTTTGATTTTAAAGGGTATGCACAACTCTTTCCCATGATTTGAAATTTCACATTGTTGCAATTGCTTTTATTTTGAAGGGTATACACAACATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAGAGTTTTTATTTTGAAGGGTATACACAATATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAATATTTCCATTTCGAAGGGTACACACAAC

>NZ_AP018042|7|3|3856765-3857976|CRT
TTGTAATTGCTCTTATTTTGAAGGGTATACACA	ACGCTATATATCTGTATGTTGCAAAACATGTTG
TTGTAATTGCTCTTATTTTGAAGGGTATACACA	ACACTAACTACACACAAAATAATTGCTAGAAAG
TTGTAATTGCTCTTATTTTGGAGGGTATACACA	ACGTTGAATACATATACTGACTAAGTATTTTAG
TTGTAATTGTCTTTATTTTGAAGGGTATACACA	ACTAAGTTGGGTTATTGTGTTGTTTAAGTTCTG
TTGTAAATGCTCTTATTTTGAAGGGTATACACA	ACATGAAATAAGTTATTGTCACTTGAATTCCAG
TTGTAATTGCTCTTATTTTGAAGAGTATGCACA	ACTTATCAACGGGTTTGCCCGATAAACCTTTTG
TTGTACTTGCTCTTATTTTGAAGGGTACACACA	ACTTTGATTTTTGCATAAAAGAAATAAAATCTG
GTGTAACTGCTCTTATTTTGAAGGGTATACACA	ACACGTATAGCAAAGAGTTTTGTGACAAACATG
TTGTAATTGCTTTTATTTAGAAGGGTATACACA	ACTTGTAGGTTAATACTCCATCAATGAAGAAAG
TTGTAGTTGCTCTTATTTTGAAGGGTATACACA	TTGTAATTTTTTAAATCTCTAATCATATCGG
TTGTAATTGCTCTTATTTTGAAGGGTATAAACA	ACATTCATTGCCCCGTGCTTTAGCTCGGGGTT
TTGTGAAAGTTTTGATTTTGAAGGGATACACAA	CAATTTTCAATGCTCATAATTTTAATTTTGAGT
TGTACTTGCTCTTATTTAGAAGGGTATACACAA	CAGTTAATGATGCGTTCAAAGAGATTTTATTGT
TGTAACTGCTCTTATTTTGAAGGGTATACACAA	CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
TGTGAAAGTTTTGATTTTGAAGAGTATGCACAA	CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
TGTGAAGGTTTTGATTTTAAAGGGTATGCACAA	CTCTTTCCCATGATTTGAAATTTCACATTGT
TGCAATTGCTTTTATTTTGAAGGGTATACACAA	CATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
TGTGAGAGTTTTTATTTTGAAGGGTATACACAA	TATCCATTGCCCCGTGCTTTAGCTCGGGGTTT
TGTGAATATTTCCATTTCGAAGGGTACACACAA

>NZ_AP018042|7|1|3856777-3857587|PILER-CR
TTATTTTGAAGGGTATACACAAC	GCTATATATCTGTATGTTGCAAAACATGTTGTTGTAATTGCTC
TTATTTTGAAGGGTATACACAAC	ACTAACTACACACAAAATAATTGCTAGAAAGTTGTAATTGCTC
TTATTTTGGAGGGTATACACAAC	GTTGAATACATATACTGACTAAGTATTTTAGTTGTAATTGTCT
TTATTTTGAAGGGTATACACAAC	TAAGTTGGGTTATTGTGTTGTTTAAGTTCTGTTGTAAATGCTC
TTATTTTGAAGGGTATACACAAC	ATGAAATAAGTTATTGTCACTTGAATTCCAGTTGTAATTGCTC
TTATTTTGAAGAGTATGCACAAC	TTATCAACGGGTTTGCCCGATAAACCTTTTGTTGTACTTGCTC
TTATTTTGAAGGGTACACACAAC	TTTGATTTTTGCATAAAAGAAATAAAATCTGGTGTAACTGCTC
TTATTTTGAAGGGTATACACAAC	ACGTATAGCAAAGAGTTTTGTGACAAACATGTTGTAATTGCTT
TTATTTAGAAGGGTATACACAAC	TTGTAGGTTAATACTCCATCAATGAAGAAAGTTGTAGTTGCTC
TTATTTTGAAGGGTATACACATT	GTAATTTTTTAAATCTCTAATCATATCGGTTGTAATTGCTC
TTATTTTGAAGGGTATAAACAAC	ATTCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTT
TGATTTTGAAGGGATACACAACA	ATTTTCAATGCTCATAATTTTAATTTTGAGTTGTACTTGCTCT
TATTTAGAAGGGTATACACAAC

>NZ_AP018042|7|6|3856777-3857977|CRISPRCasFinder
TTATTTTGAAGGGTATACACAAC	GCTATATATCTGTATGTTGCAAAACATGTTGTTGTAATTGCTC
TTATTTTGAAGGGTATACACAAC	ACTAACTACACACAAAATAATTGCTAGAAAGTTGTAATTGCTC
TTATTTTGGAGGGTATACACAAC	GTTGAATACATATACTGACTAAGTATTTTAGTTGTAATTGTCT
TTATTTTGAAGGGTATACACAAC	TAAGTTGGGTTATTGTGTTGTTTAAGTTCTGTTGTAAATGCTC
TTATTTTGAAGGGTATACACAAC	ATGAAATAAGTTATTGTCACTTGAATTCCAGTTGTAATTGCTC
TTATTTTGAAGAGTATGCACAAC	TTATCAACGGGTTTGCCCGATAAACCTTTTGTTGTACTTGCTC
TTATTTTGAAGGGTACACACAAC	TTTGATTTTTGCATAAAAGAAATAAAATCTGGTGTAACTGCTC
TTATTTTGAAGGGTATACACAAC	ACGTATAGCAAAGAGTTTTGTGACAAACATGTTGTAATTGCTT
TTATTTAGAAGGGTATACACAAC	TTGTAGGTTAATACTCCATCAATGAAGAAAGTTGTAGTTGCTC
TTATTTTGAAGGGTATACACATT	GTAATTTTTTAAATCTCTAATCATATCGGTTGTAATTGCTC
TTATTTTGAAGGGTATAAACAAC	ATTCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTT
TTGATTTTGAAGGGATACACAAC	AATTTTCAATGCTCATAATTTTAATTTTGAGTTGTACTTGCTC
TTATTTAGAAGGGTATACACAAC	AGTTAATGATGCGTTCAAAGAGATTTTATTGTTGTAACTGCTC
TTATTTTGAAGGGTATACACAAC	ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAAGTTT
TGATTTTGAAGAGTATGCACAAC	ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAAGGTTT
TGATTTTAAAGGGTATGCACAAC	TCTTTCCCATGATTTGAAATTTCACATTGTTGCAATTGCTT
TTATTTTGAAGGGTATACACAAC	ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAGAGTTT
TTATTTTGAAGGGTATACACAAT	ATCCATTGCCCCGTGCTTTAGCTCGGGGTTTTGTGAATATTT
CCATTTCGAAGGGTACACACAAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096430525.1|3862121_3864326_+|catalase/peroxidase-HPI	unknown	unknown	gnl|CDD|237891
NZ_AP018042.1|WP_096430533.1|3867468_3868020_-|hypothetical-protein	unknown	unknown	gnl|CDD|368317
NZ_AP018042.1|WP_096430491.1|3846562_3847492_+|EamA-family-transporter	unknown	unknown	gnl|CDD|273359
NZ_AP018042.1|WP_096430507.1|3853333_3856690_+|hypothetical-protein	cas13b	cas13b_CAS_V-B1	gnl|CDD|380475
NZ_AP018042.1|WP_096430541.1|3869489_3870350_-|AraC-family-transcriptional-regulator	unknown	unknown	gnl|CDD|380381
NZ_AP018042.1|WP_096430481.1|3842647_3844705_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096430519.1|3859867_3860932_-|zinc-metalloprotease	unknown	unknown	gnl|CDD|239802
NZ_AP018042.1|WP_096430527.1|3865274_3866288_-|UDP-N-acetylmuramate-dehydrogenase	unknown	unknown	gnl|CDD|234593
NZ_AP018042.1|WP_145957647.1|3858865_3859600_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096430499.1|3850159_3851062_+|NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl|CDD|223528
NZ_AP018042.1|WP_096430493.1|3847833_3848718_+|AraC-family-transcriptional-regulator	unknown	unknown	gnl|CDD|378964
NZ_AP018042.1|WP_162845451.1|3844826_3845474_+|ankyrin-repeat-domain-containing-protein	unknown	unknown	gnl|CDD|293786
NZ_AP018042.1|WP_096430538.1|3868597_3869470_+|metallophosphoesterase	unknown	unknown	gnl|CDD|277317
NZ_AP018042.1|WP_096430496.1|3848806_3849796_+|aldo/keto-reductase	unknown	unknown	gnl|CDD|381302
NZ_AP018042.1|WP_096430530.1|3866319_3867345_-|low-specificity-L-threonine-aldolase	unknown	unknown	gnl|CDD|99748
NZ_AP018042.1|WP_096430505.1|3852603_3853329_+|hypothetical-protein	Csx27	-	unknown
NZ_AP018042.1|WP_096430535.1|3868144_3868483_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096430522.1|3861660_3862086_+|transcriptional-repressor	unknown	unknown	gnl|CDD|133478
NZ_AP018042.1|WP_096430488.1|3845547_3846264_+|DUF3592-domain-containing-protein	unknown	unknown	gnl|CDD|378822
NZ_AP018042.1|WP_096430502.1|3851360_3852068_+|ZIP-family-metal-transporter	unknown	unknown	gnl|CDD|223505

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_AP018042.1|WP_096430505.1|3852603_3853329_+|hypothetical-protein	Csx27	-	9.2e-45	147.9	16.3
NZ_AP018042.1|WP_096430507.1|3853333_3856690_+|hypothetical-protein	cas13b	cas13b_CAS_V-B1	2.2e-141	467.6	60.6

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096430525.1|3862121_3864326_+|catalase/peroxidase-HPI	gnl|CDD|237891	PRK15061, PRK15061, catalase/peroxidase.	0
NZ_AP018042.1|WP_096430533.1|3867468_3868020_-|hypothetical-protein	gnl|CDD|368317	pfam05176, ATP-synt_10, ATP10 protein. ATP 10 is essential for the assembly of a functional mitochondrial ATPase complex.	1.20563e-06
NZ_AP018042.1|WP_096430491.1|3846562_3847492_+|EamA-family-transporter	gnl|CDD|273359	TIGR00950, Uncharacterized_inner_membrane_transporter_YicL, Carboxylate/Amino Acid/Amine Transporter. [Transport and binding proteins, Amino acids, peptides and amines].	5.3362e-19
NZ_AP018042.1|WP_096430507.1|3853333_3856690_+|hypothetical-protein	gnl|CDD|380475	cd20477, Cas13b_Pb-like, Class 2 type VI-B CRISPR-associated RNA-guided ribonuclease Cas13b from Prevotella buccae and similar Cas13b proteins. CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) adaptive immune systems defend microbes against foreign nucleic acids via RNA-guided endonucleases. These systems are divided into two classes: class 1 systems utilize multiple Cas proteins and CRISPR RNA (crRNA) to form an effector complex while class 2 systems employ a large, single effector with crRNA to mediate interference. Class 2 type VI CRISPR-Cas13 systems use a single enzyme to target RNA using a programmable crRNA guide and are divided into four subtypes based on the identity of the Cas13 protein (Cas13a-d). The Cas13 proteins are capable of both pre-crRNA processing and target RNA cleavage, which protect the host from phage attacks. Once bound to a target RNA, their non-specific RNase activity is activated. Cas13b has many distinctive features compared to the other Cas13 proteins, including the lack of significant sequence similarity, disparate crRNA repeat region, and double-sided protospacer flanking sequence (PFS)-dependent target RNA cleavage.	0
NZ_AP018042.1|WP_096430541.1|3869489_3870350_-|AraC-family-transcriptional-regulator	gnl|CDD|380381	cd06976, cupin_MtlR-like_N, AraC/XylS family transcriptional regulators similar to MtlR, N-terminal cupin domain. MtlR is a Pseudomonas fluorescens protein that acts as a transcriptional regulator of the mannitol utilization genes. It has an N-terminal cupin domain (represented by this alignment) and a C-terminal AraC/XylS family helix-turn-helix (HTH) DNA-binding domain. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.	5.28131e-35
NZ_AP018042.1|WP_096430527.1|3865274_3866288_-|UDP-N-acetylmuramate-dehydrogenase	gnl|CDD|234593	PRK00046, murB, UDP-N-acetylmuramate dehydrogenase.	0
NZ_AP018042.1|WP_096430519.1|3859867_3860932_-|zinc-metalloprotease	gnl|CDD|239802	cd04275, ZnMc_pappalysin_like, Zinc-dependent metalloprotease, pappalysin_like subfamily. The pregnancy-associated plasma protein A (PAPP-A or pappalysin-1) cleaves insulin-like growth factor-binding proteins 4 and 5, thereby promoting cell growth by releasing bound growth factor. This model includes pappalysins and related metalloprotease domains from all three kingdoms of life. The three-dimensional structure of an archaeal representative, ulilysin, has been solved.	5.84673e-66
NZ_AP018042.1|WP_096430499.1|3850159_3851062_+|NAD(P)-dependent-oxidoreductase	gnl|CDD|223528	COG0451, WcaG, Nucleoside-diphosphate-sugar epimerases [Cell envelope biogenesis, outer membrane / Carbohydrate transport and metabolism].	7.87349e-43
NZ_AP018042.1|WP_096430502.1|3851360_3852068_+|ZIP-family-metal-transporter	gnl|CDD|223505	COG0428, COG0428, Predicted divalent heavy-metal cations transporter [Inorganic ion transport and metabolism].	4.37384e-12
NZ_AP018042.1|WP_096430493.1|3847833_3848718_+|AraC-family-transcriptional-regulator	gnl|CDD|378964	pfam12833, HTH_18, Helix-turn-helix domain.	1.78184e-18
NZ_AP018042.1|WP_162845451.1|3844826_3845474_+|ankyrin-repeat-domain-containing-protein	gnl|CDD|293786	sd00045, ANK, ankyrin repeats. Ankyrin repeats are one of the most abundant repeat motifs, and generally function as scaffolds for protein-protein interactions in processes including cell cycle, transcriptional regulation, signal transduction, vesicular trafficking, and inflammatory response. Although predominantly found in eukaryotic proteins, they are also found in some bacterial and viral proteins. Less is known of their physiological roles in prokaryotes. Some bacterial ANK proteins play key roles in microbial pathogenesis by mimicking or manipulating host function(s). The pathogen Providencia alcalifaciens N-formyltransferase ankyrin repeats function in small molecule binding and allosteric control. Ankyrin-repeat proteins have been associated with a number of human diseases.	4.98581e-13
NZ_AP018042.1|WP_096430538.1|3868597_3869470_+|metallophosphoesterase	gnl|CDD|277317	cd00838, MPP_superfamily, metallophosphatase superfamily, metallophosphatase domain. Metallophosphatases (MPPs), also known as metallophosphoesterases, phosphodiesterases (PDEs), binuclear metallophosphoesterases, and dimetal-containing phosphoesterases (DMPs), represent a diverse superfamily of enzymes with a conserved domain containing an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. This superfamily includes: the phosphoprotein phosphatases (PPPs), Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	1.08937e-08
NZ_AP018042.1|WP_096430496.1|3848806_3849796_+|aldo/keto-reductase	gnl|CDD|381302	cd19076, AKR_AKR13A_13D, AKR13A and AKR13D families of aldo-keto reductase (AKR). Schizosaccharomyces pombe aldo-keto reductase YakC is a founding member of aldo-keto reductase family 13 member A1 (AKR13A1). It catalyzes the reversible reduction of ketones to the respective alcohols using NADP(+) as a hydride donor. Rauvolfia serpentina PR is a founding member of aldo-keto reductase family 13 member D1 (AKR13D1). It catalyzes the NADPH-dependent reduction of the aldehyde perakine to yield the alcohol raucaffrinoline in the biosynthetic pathway of ajmaline in Rauvolfia, a key step in indole alkaloid biosynthesis. This family also includes Arabidopsis thaliana aldo-keto reductases, ALKR1-6.	3.93523e-163
NZ_AP018042.1|WP_096430530.1|3866319_3867345_-|low-specificity-L-threonine-aldolase	gnl|CDD|99748	cd06502, TA_like, Low-specificity threonine aldolase (TA). This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). TA catalyzes the conversion of L-threonine or L-allo-threonine to glycine and acetaldehyde in a secondary glycine biosynthetic pathway.	1.64544e-128
NZ_AP018042.1|WP_096430522.1|3861660_3862086_+|transcriptional-repressor	gnl|CDD|133478	cd07153, Fur_like, Ferric uptake regulator(Fur) and related metalloregulatory proteins; typically iron-dependent, DNA-binding repressors and activators. Ferric uptake regulator (Fur) and related metalloregulatory proteins are iron-dependent, DNA-binding repressors and activators mainly involved in iron metabolism. A general model for Fur repression under iron-rich conditions is that activated Fur (a dimer having one Fe2+ coordinated per monomer) binds to specific DNA sequences (Fur boxes) in the promoter region of iron-responsive genes, hindering access of RNA polymerase, and repressing transcription. Positive regulation by Fur can be direct or indirect, as in the Fur repression of an anti-sense regulatory small RNA. Some members sense metal ions other than Fe2+. For example, the zinc uptake regulator (Zur) responds to Zn2+, the manganese uptake regulator (Mur) responds to Mn2+, and the nickel uptake regulator (Nur) responds to Ni2+. Other members sense signals other than metal ions. For example, PerR, a metal-dependent sensor of hydrogen peroxide. PerR regulates DNA-binding activity through metal-based protein oxidation, and co-ordinates Mn2+ or Fe2+ at its regulatory site. Fur family proteins contain an N-terminal winged-helix DNA-binding domain followed by a dimerization domain; this CD spans both those domains.	3.31617e-28
NZ_AP018042.1|WP_096430488.1|3845547_3846264_+|DUF3592-domain-containing-protein	gnl|CDD|378822	pfam12158, DUF3592, Protein of unknown function (DUF3592). This family of proteins is functionally uncharacterized.This family of proteins is found in bacteria, archaea, eukaryotes and viruses. Proteins in this family are typically between 150 and 242 amino acids in length.	4.53876e-20

Protein sequences

>NZ_AP018042.1|WP_096430507.1|3853333_3856690_+|hypothetical-protein
METIEKAEHKGLNVYKTLKSDPQYFGGYLNMARSNIFSINNSVAHKIKISTLPDEEKILDSFLCNNNRKHLNWNLAHSIAVKFLPIIKVFDFESLPKSERTGDLNNLNTGKDFAAMAASLRCLFREIQEFRNDYSHYYSIANGTKRKTIISGELAEFLRLNFARAIEYTKERFNGVLNDDDFEPVKERVLVNQDNTITTDGFVFLISMFLEREHAFQFIGEIKGLKGTQFNSFIATREVLMAFCAKLPHDRFVSEDKKQAFTLDIINTLNRCPKELYGVITDEERKAFKPCLDKLNLENLLDNSTNDKADVEDYDKYIEALTRKIRHSNRFSYFALKFIDETDIFSKLRFQINLGKLLIDEYEKPINREMYPRSIVQDVKAFGKLSDFEDGAEVLKQIDKDGNSLGFEQYAPFYHTKNNKIGLHTNNAKAIVIHKPNSQSKVKKSLKQALPEAFLSLHELPKIIVLEHLEKGKSEELINDFVLACNSKITNKQFIDEVKRKLPNDWNEFSKRSDSQKESAYKPNALAYLRMRKKIVDEVLAPYNLNHKQIPTRILDYWLCIVDINADRAISDRIKRMKREGMDRLKAYQKFTKTGKGKIPKIGEMATFLAKDIVDMVISTDKKKKITSFYYDKMQECLALFADPEKKSLFVDLISKELHLNEADGHPFLKNIEFSKIRYTQDLYLIYLQEKVNKMIDVKNFRSGKTSTIDKSWMMCTFYKREWNQEAGKQLTEVKLPDNLSSIPFTLRQLKEKISSNLDEWLHNLTKGNEANDGKTPINLPTNLFDEPLIRLLQNDLDAQQVEYSADAKYNELFKIWWRNRGDSTQSFYHAEREYFIFDEKVNFKLQENAMFADFYSLSVKKAFRAKQNARRIEQKTNRRLPDIQFSQVEKVFKRSISNTEKQIRLLQEEDQILLLMLEKLISSDQDLNLKLNQIDILLNETITVKEPVRGNLFFDNNAEITRRIIDQRKRKDHSMLHKYVFDRRLPELFEYFAENEIPLQDLKNELEAYNNAKQMVLATVFKFEEALVANNHADDFMDADSKTGHIPHKAYLQWLKKEGMINENEFQFLNRVRNCFSHNQFPPKNTMSLFVSQWADGNFALQIAELYNEKIDAILAI
>NZ_AP018042.1|WP_096430505.1|3852603_3853329_+|hypothetical-protein
MGKFSLYDLLGLLLPGVLFLFFSGVISHIYGLLPAFLGVLNWQVNIGITLCFALIIGATLYATNFHLVKKSWYNRLLGMYKHVTVLYLKMKFLHQLMNETLNIKSKEWYGKNIFFNKADFDILTAEKQKETEDLQDEFYDRMYYELEYHGKIEHAKTVQSFHFFFRQTALACIILLLLNIVLFALYFIFKPHTRKSDIFATLLLDGLLFSILFVSARLAQWYRKRMVMKMYWAYFTHLKQI
>NZ_AP018042.1|WP_096430502.1|3851360_3852068_+|ZIP-family-metal-transporter
MLQKIILFSGFAGVTVFIGGLLAICFKHHIKESPIKYEIIHTLKSFGAGIILSALALVLIPKGMEELELFPLAISFLAGTIIFMFIDRYLAKKGGKTATLLAMMMDFIPEAIALGALFAIEPDMATLLAVFIGLQNLPESFNAFRDLVQSGYPVKKTLVIFFFLSFFGIIGALIGHFFLSGYPDLTAHLMTFASGGILYLLIQDIIPDSKLKKNYLTSLGASLGFLVGIILAGIV
>NZ_AP018042.1|WP_096430499.1|3850159_3851062_+|NAD(P)-dependent-oxidoreductase
MTKKRIFFTGGSGKAGKHVIPYLLDQGHRVMNVDLVPLDCPGVDNLVADITDSGQIFNAMSSYAGLDELESGKGVPKFDAVVHFAAVPRILLKPDNETFRINTIGTYNVIEAAVKLGIKKIIIASSETTYGICFSDGKTNPTALPLEEDYDVDPMDSYGLSKVVNEKTARSFQRRSGFDIYALRIGNVIEPHEYTELFPHYFEKPEVRRRNAFCYIDARDLGQIVDLCLNKDELGYQVFNAGNDHNGAIIPSKELAKQFFPDVPITRELEEHEALFSNRKIREVLGFKEQHNWQKYLKKD
>NZ_AP018042.1|WP_096430496.1|3848806_3849796_+|aldo/keto-reductase
MKYIKLGKTDDQLSIIGLGCMGMSHVYGKKNNEESIATMHKALELGINFWDTADFYGGGANEQLISKVLKPNRDKIFIATKFGFQYGGEDLLASKFDGSPKYMRKAVELSLQRLGIETIDLYYAHRVDPNIPIEETVGAMAELVKEGKVRHLGLSEASLSNIKRANAVHPISALQSEYSLLTRDVEKKVLPEIAEMGITLVPYSPLGRGMFTENFNVDDIASNDIRMHMPRFQGKHLINNQNLMKALDELAYGKCINTAQLALAWLLAKGDNIIPIPGTKKRKYLGLNAGAADIEFTANEVAAIEAIIAKYPNTGERYPEAAMRLVDQD
>NZ_AP018042.1|WP_096430493.1|3847833_3848718_+|AraC-family-transcriptional-regulator
MEKSEDLNAFYDRIKYKPDEELSLSKPHINVFERSACTGPLTYSRRDYYKLTLILGEGRLDYADKSIHIDRPALLFSNPLIPYNWQNISKEQTGWFCIFNEFFVKQRDELLTDLPMFQLGYDKVYFPDENSVKEITDLYRKMMAENEGSYIYKQDVLRNYLHLIVHYALKMEPAKNDAQTQNAASRIASLFMELLERQFPIDSMQNQLKLKSANDYACQLSVHTNHLNRAVKEVTGKTTTEHIAGRTIIEAKELLVHTDWPVAEIAYCLGFEYPAYFNNFYKKATGMTPKEARL
>NZ_AP018042.1|WP_096430491.1|3846562_3847492_+|EamA-family-transporter
MIGAQTKGTIMVLLSAIFFGLMPFFAIKTYAEGFDVSNLLFYRFSFAFVLIGLFCIYKKISPRINKKQFFLLLIAAFGGTMMTTYTLFLSYQYISSGLASTLHFVYPIITIVFAGIIYKEKFTAKKIIALVLSIIGIALLSVGGGAEINTIGVLWALVSGLFYAIYILCMANPELRKISPYSVALWVFGITALLFFGKCLFVDGIKTNPSAMSLFYILNLSFWSTFLSVVLFYKGMKYTGPGNASLLSTLEPLTGVMVGVFVFNDVMDLKSSLAILLILTSVFIVVQTNKKPKYRMLFRKLTPRRLRRA
>NZ_AP018042.1|WP_096430488.1|3845547_3846264_+|DUF3592-domain-containing-protein
MKKKTIKIISYLFAGVGIIFLLFGMNGTWDNISFIKKSTETLGTVVDMKVKDKTDSEGVSQTYYYPVVEYTDANGAQHVLNSSMGSGRPECEIGEGIQVLYLPENPSDGKIGTFGHMWGGMLIVWILAIVFIITGFLIEFFSKRDKRRKKKAERYSATVDAKVSEVIYNTSVEVNGRSPFQIHAQWHDEQSNKIYIFKSKNFWFDPSEFLHDTIAVKADPANYKKYWMDTSFIPEMAE
>NZ_AP018042.1|WP_162845451.1|3844826_3845474_+|ankyrin-repeat-domain-containing-protein
MIMTTEPWSNSFDDVFEILERGADIEYIDSSKKAYTTPFLNASGALKCLREKDQRRPGEIDSMEIEAVKIVKYLAKKGADIHATTSGNNLNALHLAANGGREKMIPVLVKLGLDINSRNGDPDYGGTVLWHAINAGDLATVKAVVEAGADINLCLLDGNSPLDWAMAYANPKAKEALWLVPYRDQEAIADYMQSIGAKHGKNQFKGMLYFDESKR
>NZ_AP018042.1|WP_096430481.1|3842647_3844705_+|hypothetical-protein
MAYIELEIEGQTQKYEYEESIQGEVVYDINTNEVGIIREDQRSSFKQEVDWMDAFTPYMASFQKKEQDEAKEEDRKYLALATLIIYDEFVNASNDATAADIINHLDAKINEKKEELKADGPIDTSAVNISDKVHSEILWAEGKRMVRVRSEKIKNHNRWFGKKQVQQQIEAGNESENEKTAKTAKSAEIKILEKKFWSEDGAINDKFEAINKDLHKDFFEDNNNINSSLDAKLFRYTTEAAADTKFDWTDKKELKATAKASGSFSLAEATGKFSVALPDQYGFGLMQYCKSIAPDFIDDDYTEIFLKIILGLDGSAFVGICASVSGEIGLSVTEGKENGSAQAGLDIFAGAKGEAKASFEIKMMFVEDYVMDAMRDRLKTVGERGETVVSSKEIEADKLSKWEKLGSAEIGGFVAAGAGISGVFAVGYFDGQLRYQAKIALVVKVGGGTFMKGFVDAKLVGKFILTVAHGLNWKNMSDALDKQTELLYHTIMNNCFYLQRTVAEVYGELEGKFKEIMDTIQDLRDLPEKGLEQFKIADDNWDENVPGYSSFKQYNLAFLVLKSTYYSLKQRNENLSEKEAAIALVEKAQQNKISWNYATWQMKIDLIYDMRHDGSGAFGGFSEEDKEDAVIAVLESSRNSEEFKKIEENLKTPKQGTGREAIFIDDLVDFSQQDRLDYLRNIKYR
>NZ_AP018042.1|WP_145957647.1|3858865_3859600_+|hypothetical-protein
MKKVHANAITNEIAEICKGIIELSKRYPIELDDYFIFIFQKLSGKSVELVGKINAGWLNSELKEKDDARDLDVRAIFYEVKAKCMRRPSADQEKALRIKEVLDRYGMKITEDSYTIESAGVRAMLSDLNAPELEDCVASNADFKILLDNAEQSMAEFDVSASKLIEDKNERENAKSASVVGQELKGIINNELLIYLAAMSKANPTKYKPYADVVNTLIEDSNNKVRDRLAAFKRKKEKINPELN
>NZ_AP018042.1|WP_096430519.1|3859867_3860932_-|zinc-metalloprotease
MKKIIFSMACLALILGSCQKNANDITPEQQEIAVDMSDFYVYTSDDNTVKSGHVHGPKCGSMTVLNRQLKENPGLENRLYAIEKHTRQLIAAKSSSSTNKGKPVKPGDTPDPTPYQGSITIPVIINILEDANHPVTQANIDSQIDILNDDYNDLNPATGAVPSEFNNAIANVDITFVLAGINRKVSTKTSWGTNDAMKLSSQGGIDATDPSFYLNFWICEIGGGILGYAQFPGGALATDGVVIGSDYFGRNTLGGQYGYGRTATHEVGHWLNLRHIWGDGNCRQDDFVTDTPGSDQANYGCPSYPTVNCRSNDMTMNYMDYVDDQCMYMFTNGQNDRIRAIFAAGGERESFVIQ
>NZ_AP018042.1|WP_096430522.1|3861660_3862086_+|transcriptional-repressor
MKVGEISAKLNEKGLKVTPQRIAVFEAISKLSNHPTADNVIDYIRNKHPNIATATVYKVLDALVSNELIKKVKTEKDVMRYDAILEKHHHLYCSESDRIEDFNDEELNNILEMYFKKKEIPDFKIEDIKLQIIGKFTKGDQ
>NZ_AP018042.1|WP_096430525.1|3862121_3864326_+|catalase/peroxidase-HPI
MENKSDHVSKCPVTGAMGKPSSGGGTKIKDWWPNHLNLNILRQHSSLSNPMGEEFNYAEEFKSLDLKAIKNDLHKLMTDSQEWWPADFGHYGPLFIRMAWHSAGTYRTGDGRGGAGGGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNSALESMGFKTFGFGGGRADVWEPEEDVFWGNETKWLEGAERYTGERDLDNPLAAVQMGLIYVNPEGPEGNPDPVAAAKDIRETFARMAMNDEETVALIAGGHTFGKTHGAGDAAHVGAEPEAAGIEEQGLGWKNSFGSGKGGDTIGSGLEVTWTQTPAKWSYYFFENLFNYEWELTKSPAGAHQWAAKNAGKVVPDAHDASKKHLPTMLTTDLALRFDPAYEKISRRFFEHPLEFADVFARAWFKLTHRDMGPKACYLGSEVPSEELIWQDPVPAVNHALIDDKDVAELKAKVLDSGLTVSQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAANNPTQLAKVVKKLEKIQDEFNGSQVGGKKISLADLIVLAGCAAVEKAAKDAGHDVVVPFTPGRMDASQEQTDVESFVVMEPAADGFRNYLKTKYAISTEELLIDKAQLLTVTAPEMTVLVGGMRVLNANFDNSKHGVFTKRPGLLSNDFFINLLDMGTAWMPASEDKEIFDGRDRKTGAVKWTGTRADLIFGSNSELRAIAEVYGSIDANDKFVKDFVAAWNKVMNLDRFDLV
>NZ_AP018042.1|WP_096430527.1|3865274_3866288_-|UDP-N-acetylmuramate-dehydrogenase
MAALRKNVALKNYNTFGIEANARYFFEFDTTEEIQDFLSKNDVINIQYLLLGGGSNLLFTEDFNGLVIHPMVKGIKIIEEDEDSVLVQVGANEDWDAFVGWSVENNLPGIENLSLIPGVVGAAPIQNIGAYGVEVKEVIDSVEAISIESNKKVTFNNAHCEFDYRYSVFKNEYKNQFIITHVNFRFSKQAQFKVHYGAIAKELEAYDKVNLKNIREVIIKIRESKLPDPKVLGNAGSFFKNPVVAKQEADALKAKHEDMPVYELNETQSKLAAGWLIEQCDWKGKQIGDAGVHKDQALVIVNHGNAKGSEILELANEIKKSVLYKFGIKLEMEVNAI
>NZ_AP018042.1|WP_096430530.1|3866319_3867345_-|low-specificity-L-threonine-aldolase
MNKRGFASDNFSGVLPEVMEAIQNANQGHVVAYGGDPYTESAIKKFKSLFGQNIDVYFAFNGTGANVIGLSTLTQSYHSIICPATAHIQVDECGAPDKFTGCKVIPIESPDGKISPELILPHLHGFGFEHHSQPKMISITQCTELGTVYTPEEVKAICDLAHANNMYVHMDGARLANAVVSLDVDVKEFTTHAGVDVLSFGGTKNGMMLGEAVIFFNPELSKEAKYVRKQSMQLFSKMRYIGAQFDAMLTNDLWLKTARHANKMAKLLEQEVQKIDQVKITQKVQANGIWALIPKDKIEKLQEEYFFWVWDEHAGEVRWLCSFDTTEEDILGFVKLLKQVL
>NZ_AP018042.1|WP_096430533.1|3867468_3868020_-|hypothetical-protein
MIKSIAIALLLGIVIISQVTAQNPNKLKSGDLFPEIKGELLSSKMISLPDHCKGKVSVLIIAFKRGTQPQIDEWTKPLMKEFSIHSDFRFIEVPMISNLYNWMSHYIDNGMRSGIAESMHKNVMTYYGPLGSYFDYFDVQDKKLCYLFLLDKEGIIQFIAKGEPNPDNLSDLLQKIKILLETV
>NZ_AP018042.1|WP_096430535.1|3868144_3868483_-|hypothetical-protein
MLEKLLTNPEKYFWAINADGDLVLGNADLGLKAKLTITLTNKWTNLAPLVESSPGIYIGQSETYLKDSKEYEQVSELYKLIKSQLKEPSIIERMKTSKNTYTFLLNHYQEQK
>NZ_AP018042.1|WP_096430538.1|3868597_3869470_+|metallophosphoesterase
MICDIQLGRLMIVSDLHGNGLDFRQVLKVYRKLKSDGKADCLVFLGDLIHAYPGKRKDESLEMVQELIKMGANQEGSDVICLLGNHEFVHIYHIPLQRGNLEFTSWFENRIRKNREDIIRFFMDMPFMLRTKGGVLLNHTGGSNRYSETESFDFNWFKNYQHKGEFAAHIDHIHRYDPQIGSQFMSTAEGDYLWEVLMNGNERQFGEEYLNMVTDLLQFASKDREHDPLKALVSGHIGVDYGAEMIGEYKLRLCSSAGCLRDLEKKYLLIDAEKAYTNSMELLDCCHDLY
>NZ_AP018042.1|WP_096430541.1|3869489_3870350_-|AraC-family-transcriptional-regulator
MSIVLEEISTKNEHSFRSKIDVLPHIEVPWHHHPEFELIHIEKSRGTLIVGDCIDNFNDGDLIFIGPNTPHVMTNDESYFQKKSDLRVIAWVVHFKEDSFGKEFFLLPEMYRIKKFLTKSFQGVRIEGSTKYKIIQYLKTLHSSEYSQRIIILLQILQVLAQSDDLEYLASDNFVETFQQRSNQKLYQIYEFVSKNFQKKIELDEAAKIANMSKTAFCRFFKSKTKKTFSEFLNEMRINYAKKLLAEGRLSVSQIAYECGFNSPSYFNKTFKSATGKSPLQLREKE

Crispr_ID: NZ_AP018042_8

• CRISPR_ID: NZ_AP018042_8
• CRISPR_location: 4415575-4415718
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: csa3

Consensus repeat of NZ_AP018042_8

Consensus_repeat	Method
GTTATTATCTGGTTGAATGGAGCTAGAGGTATTACTTCTGCTTGCATTGTGATC	CRISPRCasFinder

spacers of NZ_AP018042_8

>8.1|4415629|36|NZ_AP018042|CRISPRCasFinder
CTGAGCACGAGTGTTTGTCTTGTCAACTTTATCATT

CRISPR arrays and Neighbor proteins around NZ_AP018042_8

CRISPR arrays

The CRISPR arrays of NZ_AP018042_8

>merge|NZ_AP018042|8|4415575-4415718|CRISPRCasFinder
GTTATTATCTGGTTGAATGGAGCTAGAGGTATTACTTCTGCTTGCATTGTGATCCTGAGCACGAGTGTTTGTCTTGTCAACTTTATCATTGTTATTATCTGGTTGAATGGAGCTAGTGGTATTACTTCTGCTTGCATTGTGATC

>NZ_AP018042|8|7|4415575-4415718|CRISPRCasFinder
GTTATTATCTGGTTGAATGGAGCTAGAGGTATTACTTCTGCTTGCATTGTGATC	CTGAGCACGAGTGTTTGTCTTGTCAACTTTATCATT
GTTATTATCTGGTTGAATGGAGCTAGTGGTATTACTTCTGCTTGCATTGTGATC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096431414.1|4425107_4425476_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431416.1|4426462_4427854_+|MFS-transporter	unknown	unknown	gnl|CDD|129794
NZ_AP018042.1|WP_096431406.1|4416931_4418533_-|hypothetical-protein	unknown	unknown	gnl|CDD|380790
NZ_AP018042.1|WP_145957667.1|4429687_4430863_-|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl|CDD|214866
NZ_AP018042.1|WP_096431396.1|4411604_4411967_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096433733.1|4431109_4433965_+|DUF4982-domain-containing-protein	unknown	unknown	gnl|CDD|225789
NZ_AP018042.1|WP_096431384.1|4405279_4406431_+|iron-containing-alcohol-dehydrogenase	unknown	unknown	gnl|CDD|224890
NZ_AP018042.1|WP_096431408.1|4418623_4420303_-|hypothetical-protein	unknown	unknown	gnl|CDD|378996
NZ_AP018042.1|WP_096431402.1|4414270_4414672_-|VOC-family-protein	unknown	unknown	gnl|CDD|319924
NZ_AP018042.1|WP_096431390.1|4407548_4408622_+|ACR3-family-arsenite-efflux-transporter	unknown	unknown	gnl|CDD|213563
NZ_AP018042.1|WP_096431418.1|4428469_4429657_+|hypothetical-protein	unknown	unknown	gnl|CDD|182912
NZ_AP018042.1|WP_162845480.1|4421995_4425103_-|hypothetical-protein	unknown	unknown	gnl|CDD|379224
NZ_AP018042.1|WP_096431410.1|4420901_4421417_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431386.1|4406591_4406921_+|winged-helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	gnl|CDD|238042
NZ_AP018042.1|WP_162845478.1|4408776_4410327_+|HAMP-domain-containing-histidine-kinase	unknown	unknown	gnl|CDD|223715
NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein	unknown	unknown	gnl|CDD|227807
NZ_AP018042.1|WP_162845479.1|4412038_4413085_+|glycoside-hydrolase	unknown	unknown	gnl|CDD|236673
NZ_AP018042.1|WP_096431394.1|4410419_4411106_+|response-regulator-transcription-factor	unknown	unknown	gnl|CDD|223816
NZ_AP018042.1|WP_096431388.1|4407126_4407549_+|arsenate-reductase-ArsC	unknown	unknown	gnl|CDD|319973
NZ_AP018042.1|WP_145957666.1|4413113_4414142_+|glycoside-hydrolase	unknown	unknown	gnl|CDD|236673

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_AP018042.1|WP_096431386.1|4406591_4406921_+|winged-helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	1.9e-12	42.4	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096431416.1|4426462_4427854_+|MFS-transporter	gnl|CDD|129794	TIGR00711, Uncharacterized_MFS-type_transporter_YhcA, drug resistance transporter, EmrB/QacA subfamily. This subfamily of drug efflux proteins, a part of the major faciliator family, is predicted to have 14 potential membrane-spanning regions. Members with known activities include EmrB (multiple drug resistance efflux pump) in E. coli, FarB (antibacterial fatty acid resistance) in Neisseria gonorrhoeae, TcmA (tetracenomycin C resistance) in Streptomyces glaucescens, etc. In most cases, the efflux pump is described as having a second component encoded in the same operon, such as EmrA of E. coli. [Cellular processes, Toxin production and resistance, Transport and binding proteins, Other].	4.38497e-51
NZ_AP018042.1|WP_096431406.1|4416931_4418533_-|hypothetical-protein	gnl|CDD|380790	cd20220, PFM_natterin-3-like, pore-forming module of Thalassophryne nattereri fish venom natterins 1-4, and similar aerolysin-type beta-barrel pore-forming proteins. This group includes 4 of the 5 Thalassophryne nattereri fish venom natterins: natterin-1, -2, -3, and 4. Natterins have kininogenase activity, kallikrein activity, and are allodynic and edema inducing. They also cleave type I and type IV collagen, resulting in necrosis of the affected cells. Contradictory to their edematic activity, Natterins also have anti-inflammatory effects through inhibition of interactions between leukocytes and the endothelium, and reduction in neutrophil accumulation. Many proteins belonging to this group have an N-terminal DUF3421 domain. They belong to the aerolysin family of beta-pore-forming proteins (beta-PFPs). PFPs are generally secreted as water-soluble monomers, which upon binding to target lipid membranes, oligomerize and form transmembrane pores harmful to cells. Beta-PFPs form pores by transmembrane beta-barrels. Aerolysin-type beta-PFPs are believed to use an amphipathic beta-hairpin to form the beta-barrel, are found in all kingdoms of life and many are bacterial toxins. In addition to having a role in microbial infection, they have potential as biotechnological sensors and delivery systems. They share a similar monomeric architecture, with a variable membrane-binding domain and a structurally conserved pore-forming region. A significant portion of the monomeric subunit structure is re-organized to form the pore. Oligomers formed by members of the aerolysin family include: hepta- (aerolysin), octa- (Dln1), and nonameric oligomers (lysenin and monalysin).	6.22046e-05
NZ_AP018042.1|WP_145957667.1|4429687_4430863_-|helix-turn-helix-domain-containing-protein	gnl|CDD|214866	smart00862, Trans_reg_C, Transcriptional regulatory protein, C terminal. This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding.	0.000407587
NZ_AP018042.1|WP_162845478.1|4408776_4410327_+|HAMP-domain-containing-histidine-kinase	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	4.32456e-50
NZ_AP018042.1|WP_096433733.1|4431109_4433965_+|DUF4982-domain-containing-protein	gnl|CDD|225789	COG3250, LacZ, Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].	1.43559e-56
NZ_AP018042.1|WP_096431384.1|4405279_4406431_+|iron-containing-alcohol-dehydrogenase	gnl|CDD|224890	COG1979, COG1979, Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family [Energy production and conversion].	0
NZ_AP018042.1|WP_096431408.1|4418623_4420303_-|hypothetical-protein	gnl|CDD|378996	pfam12950, TaqI_C, TaqI-like C-terminal specificity domain. This domain is found at the C-terminus of the TaqI protein and is involved in DNA-binding and substrate recognition.	0.00275027
NZ_AP018042.1|WP_096431402.1|4414270_4414672_-|VOC-family-protein	gnl|CDD|319924	cd07263, VOC_like, uncharacterized subfamily of vicinal oxygen chelate (VOC) family. The vicinal oxygen chelate (VOC) superfamily is composed of structurally related proteins with paired beta.alpha.beta.beta.beta motifs that provide a metal coordination environment with two or three open or readily accessible coordination sites to promote direct electrophilic participation of the metal ion in catalysis. VOC domain is found in a variety of structurally related metalloproteins, including the bleomycin resistance protein, glyoxalase I, and type I ring-cleaving dioxygenases. A bound metal ion is required for protein activities for the members of this superfamily. A variety of metal ions have been found in the catalytic centers of these proteins including Fe(II), Mn(II), Zn(II), Ni(II) and Mg(II). The protein superfamily contains members with or without domain swapping. The proteins of this family share three conserved metal binding amino acids with the type I extradiol dioxygenases, which shows no domain swapping.	7.57922e-34
NZ_AP018042.1|WP_096431390.1|4407548_4408622_+|ACR3-family-arsenite-efflux-transporter	gnl|CDD|213563	TIGR00832, Uncharacterized_transporter_slr0944, arsenical-resistance protein. The Arsenical Resistance-3 (ACR3) Family (TC 2.A.59) The first protein of the ACR3 family functionally characterized was the ACR3 protein of Saccharomyces cerevisiae. It is present in the yeast plasma membrane and pumps arsenite out of the cell in response to the pmf. Similar proteins are found in bacteria, often as part of a four gene operon with an regulatory protein ArsR, a protein of unknown function ArsH, and an arsenate reductase that converts arsenate to arsenite to facilitate transport. [Cellular processes, Detoxification, Transport and binding proteins, Anions].	1.53377e-154
NZ_AP018042.1|WP_096431418.1|4428469_4429657_+|hypothetical-protein	gnl|CDD|182912	PRK11028, PRK11028, 6-phosphogluconolactonase; Provisional.	0.00626557
NZ_AP018042.1|WP_162845480.1|4421995_4425103_-|hypothetical-protein	gnl|CDD|379224	pfam13502, AsmA_2, AsmA-like C-terminal region. This family is similar to the C-terminal of the AsmA protein of E. coli.	4.79408e-05
NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein	gnl|CDD|227807	COG5520, COG5520, O-Glycosyl hydrolase [Cell envelope biogenesis, outer membrane].	1.06334e-40
NZ_AP018042.1|WP_096431386.1|4406591_4406921_+|winged-helix-turn-helix-transcriptional-regulator	gnl|CDD|238042	cd00090, HTH_ARSR, Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to dissociate from DNA in the presence of metal ions.	9.24694e-15
NZ_AP018042.1|WP_162845479.1|4412038_4413085_+|glycoside-hydrolase	gnl|CDD|236673	PRK10340, ebgA, cryptic beta-D-galactosidase subunit alpha; Reviewed.	5.82581e-08
NZ_AP018042.1|WP_096431394.1|4410419_4411106_+|response-regulator-transcription-factor	gnl|CDD|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	9.27548e-86
NZ_AP018042.1|WP_096431388.1|4407126_4407549_+|arsenate-reductase-ArsC	gnl|CDD|319973	cd16345, LMWP_ArsC, Arsenate reductase of the LMWP family. Arsenate reductase plays an important role in the reduction of intracellular arsenate to arsenite, an important step in arsenic detoxification. The reduction involves three different thiolate nucleophiles. In arsenate reductases of the LMWP family, reduction can be coupled with thioredoxin (Trx)/thioredoxin reductase (TrxR) or glutathione (GSH)/glutaredoxin (Grx).	1.63376e-61
NZ_AP018042.1|WP_145957666.1|4413113_4414142_+|glycoside-hydrolase	gnl|CDD|236673	PRK10340, ebgA, cryptic beta-D-galactosidase subunit alpha; Reviewed.	1.13763e-05

Protein sequences

>NZ_AP018042.1|WP_096431402.1|4414270_4414672_-|VOC-family-protein
MKQSIAHVTLVVRDYDQAIEFYTNKLQFDLLEDLKMSEDHRWVLIAPKGSSSCSIMLEKAIGKEQESRIGNQTGGSVFMILQTDNFERDYKHLLDNKIRITRESSTEVYGKVCVFADLYGNLWDLIEPVKKED
>NZ_AP018042.1|WP_145957666.1|4413113_4414142_+|glycoside-hydrolase
MNRIGEHTIKWMNLLFVMLLFCGFSNETKAQDYEKVVDLNGRWKFSIGDDSKWANPNYNDSNWERVDVPSAWENQGFHGYDGFAWYRTSFTYSGSLKGQSLFLKLGNIDDVDEVFMNGKSIGRTGTFPPRFSTAYTSNREYNIPSAILRDNNVIAVRVYDDVGEGGIIRGDISIVVDRDAIPVDIDLQGKWKFKIGRSAHEDLQNAENWDDIIVPGFWENQGYKNYDGYACYALDFVADEKLIAERMVLVLGKIDDLDQVIVNGVLVGQSGDFDPSTVQNYSNTYQQLRGYYLPVNLIKKGQKNRIVVRVYDATQGGGIYTGSIGLISQDHYIKYWNKRRRK
>NZ_AP018042.1|WP_162845479.1|4412038_4413085_+|glycoside-hydrolase
MKVSILLLFLIFQVGTIQSENLKSLVNLEGNWKFSIGDDPARAIPDYDDSDWQNIFVPNNWENNGYDGYNGFAWYRKSFQIRESFNREYLYLYMGYIDDVDEVYLNGKLIGASGNFPPRMKTAYNIPRMYPVPANLIRKGGKNVIAVRVYDEIQNGGITQGPVGLFIDKDQERMDVDLTGYWDFECGNSVDKGNLACISYRKGKIFVPGFWETRGYNGVDGKAKYTKKFNYPSDMSTSEKALVLGIIDDEDEVYVNGERIRWMGYKRGGNWSGNSNYNTFRAYQIPKGVLKSSGTNVIEVTVTDHMGPGGIYRGPIGITDIDLAEEMVRKEYKDNRSSFEKFFDYWFD
>NZ_AP018042.1|WP_096431396.1|4411604_4411967_+|hypothetical-protein
MKAIKIAFFALACLLLTANLGMAITPEKQKIEKEKSMLLNKIRKSVTKIDFVDFIEVGKSEVIVLRCIVNENNRVVVSKVIGFDEELKQAVRKAMDKKIITASSGLVGEELALQFKFAMQ
>NZ_AP018042.1|WP_096431394.1|4410419_4411106_+|response-regulator-transcription-factor
MKPILIVEDEPSMLMGLKDNLEFEGYQVDVAEDGGIGLDKILGNEYSLVLLDVMLPKISGFDICKQVRKKGISTPIILLTARGEEIDKVLGLEFGADDYITKPFSLRELLARIRAVLRRTENRIESKTDSSVEIGNLSINFSSFEGFVDQQEVKMSHKEVEILNYLWDNKNTIVSRDDLLDHIWGTEYQPTSRTIDNFILKLRHKIEVDPNHAKIILTVHGVGYRLVV
>NZ_AP018042.1|WP_162845478.1|4408776_4410327_+|HAMP-domain-containing-histidine-kinase
MIKPGLKIGILLTLILVLPSLFFSAYEISNINQNEAVIDSIYSSQLESILFSVNQYSDDVISGWANQLDKGKYGLDQEMESLVTKNYSIDAFFLIDSLKHQLFSKEKIPEDSLTILTEKLEYAFAQNANEIERLQQYIKSGYQKIGVLDFNMKGKSLFAFARTVDSLSVETVLLLIDIEKFIGENLGPKIQGIAQDQFYISVIDKKRNAEIYSNVVYDEGEKNIQQRRKIWLFPAYDLGIQMKGNTIENLVRKRTRTNIIMLIVMDMILLLGAWFVYRNIRQQMKLTQIKSDFISNVSHEIRTPLALINMYSETLEMGRLPSEGKKQEYYKVINTEANRLSNMVNNILNFSKIESGKREYHFKESDINAEIEIILENYGQHLHKKGFEINFESFDDFPKLLIDKEAVSEAIINLIDNAVKYSGNTKRIEIACNTDLEFANINVIDFGIGISKKDQALIFDKFYRVSSGDLANKVKGSGIGLSIVKHIMDAHNGRITVKSSKENGTCFTLSFPLSFK
>NZ_AP018042.1|WP_096431390.1|4407548_4408622_+|ACR3-family-arsenite-efflux-transporter
MGALNKKLSFLDRYLTVWIFAAMAFGVAMGYFFPAMSGFWNKLSVGTTNVPIAIGLVIMMYPPLAKVKYEELKDVFKNRKILILSLVQNWILGPTLMFALAIIFLQDYPEYMTGLILIGLARCIAMVIVWNDLAKGDPEYAAGLVAFNSIFQVLFFSVYAYVFITVLPGFFGLEGSVVDVSMGDIAESVFIYLGIPFIAGMITRYFGIKAKGKDWYEKKFIPKISPLTLFALLFTIFVMFSLKGEYIVQLPFDVVRIAIPLVIYFVIMFMISFFMGHKFKAGYAKTASLSFTAASNNFELAIAVAIAVFGIDSGVAFAAVIGPLVEVPVLIALVNVSLKLKGKYFKGKIDGVKCANE
>NZ_AP018042.1|WP_096431388.1|4407126_4407549_+|arsenate-reductase-ArsC
MKVLVLCTGNSCRSQMGEAILREINPELEVVSAGTKIADQVHPLAVKAMAEIGIDISSLRPKMVDVFLEEGVDFLISVCGGAKDACPAFFGPVGTRLHIGFDDPAYAEGTEEEIFNVFRRVRDEIRRDFAKFNEDYILNK
>NZ_AP018042.1|WP_096431386.1|4406591_4406921_+|winged-helix-turn-helix-transcriptional-regulator
MRKLDLFEEGQQEIAAMAKVLSHPARIAILQFLAATPTCISGDISDFLPLSRTTVSQHLKDLKSAGLIKGEVEGLKIKYCLNKKGIEKLKQSFGDLLEEITPEEEKNCL
>NZ_AP018042.1|WP_096431384.1|4405279_4406431_+|iron-containing-alcohol-dehydrogenase
MENFDFYNPVNILFGKGKIAEINKHIPKDAKILMTYGGGSIKKNGVYNQVMDALKGYKIVEFGGIEPNPRFETLMKAVEVVKSEKIDFLLAVGGGSVVDGTKFIAAATYFEGDAWDILAKRAPITKAVDLGAVLTLAATGSEMNNGGVVTKAATKEKIPFSNPLLFPKFSVLDPETTYSLPKRQLTNGVVDAFVHVMEQYLTYPVNSPVQDRFAEGILLTLLEEGPKELASDTPNYERRANLMWAATMALNGLIGMGVKSDWATHMIGHELTAFHGIDHAVTLAIVLPGLLTHLKEKRGEKLLQYAERVWNITEGTDEEKKTLAIEKTEAFFNQVGIATRLSDHNVGQDSINTIAKRFEEKGYVGMLPDVEVKDVAKILQARL
>NZ_AP018042.1|WP_096431406.1|4416931_4418533_-|hypothetical-protein
MYKIIFLSFIFSIALFASCTFEVSEEPRSADIDLEVENGTTRFEGISDSTLNSIPVDAGEIGVAIDTRKLAVLGYKPSKVSFTIIGDLSTFTQSNIQIDPYTHVALFKLSRKDLTEEQITEFSEGVAIEARVFDAENVTMETMYGSKFIINSTNRTIKIESEKPHVQYPMKLNADMPYYLAYEGFSLTNYLGAEIVGNFSEMSGTFIINSTLYHNDPTNIYSKLQKFYFIEIEENIYAIKTAHKNSYLEINDKRKLAYNIENTDDNPNFVLGDKHKFILEQTAKGTIEIRSLSGGTFSFYNGGAPFTFIAANITWEFNDLGTEYSAAILPPAQMDFAFDQTIHNCSGATGEYYVGNNREETKTVSIDVEETASFYSSTTDSKEATVEAEAGGEIFGIGASVSASGTIGTSRTRAFGSGTGTNKGKTYTETQTVSSNRVITVPPYTSVEVFDVIQKLKNVRIPFAQRVLLRAANDEGIPLSGEEIQSQLGANQFGGVVLEVGSDYIEYSVRGSINVANYFEYKNTVNDIVGICN
>NZ_AP018042.1|WP_096431408.1|4418623_4420303_-|hypothetical-protein
MKIKNNALLILLIFTALLTSCKFELIQEVDEVAELELGIPGEIANGISDETLLSIPIDLGEIGVAVDTRILAIRAYKPSKISFSIEGSLSAFSQVDIPVDEFTHVANFTIKRKDLSDAELKGFSNGVPISVTVFNEVGDYIDGRTVSRYIINATNRTLKIESKLPYILKPLSFNPDIPYFMQTAADTEFKTLGISSDYEGFTGSELGLLSGKYQFYFEKTANYERDFTYKIKIYTYTWDYVDYGYLDADVFNTYGNNGGNNGEKIAVGWGVDDPTSNRMLIGESDFLLEQTNNGTVKIRLFSSESYLSTNGDPFETGYKIFFDTDPTNDLEFNIFADNISWKFDDLGTKYSPAIIPPTKMDFAFDQTIINCSGATGDYEVGIATTVSNTTSISYEESINLFSSKFDFKEATVEASAEASFFGIGASVSASGTLRTETTTEWGKEKKTNQGIEYEGSQEVSASRKVSVLPYSAIEVFDVIQKLENVRIPFVQRFIIRGSVNDEKHLTGPEIEAQLISNQFRGVVTEVGSDFIVISIRGAVNVANYFEYNNNLHDIEGACN
>NZ_AP018042.1|WP_096431410.1|4420901_4421417_+|hypothetical-protein
MAKGKTRFPLQIRIIHRYLGYFLSGIMMVYAVSGIVMIFRETNFLKNEVVLEQQLEPNLTGAELSPKLRMRVKVEKEEGDVLYFKDGNYNQKTGVVIATKMELPFVLEKMERLHKATTNSPLYFLNIFFGVSLLFFVLSAFWMYSPKMPVFKKGMYFAVGGVILTIVLLFV
>NZ_AP018042.1|WP_162845480.1|4421995_4425103_-|hypothetical-protein
MRIVKKLGITLLVLVFVVIIAAAIGLWYVFTPEKLTPIVNNQAKEYLDCTTSIEKVEPTFFSTYPFFGMELTNLCLTENNDSVKTDTLLYAKKCFASLDVLSYLFHDNIRLNPFLVENGYLNFKIDSQGNSNFDIFKSSSDSTEADSEASSFGNIDISNVVFKNFNANYTDETTFRKAAINHLNAALEFKYTDEKQYFDLDLQVNKLLFTTSDSMALCIDAKNCDLKMNSKGQDKNLFNSDLQLVCKEMSVSMAGDTILSKLKVDSHLPFSVNIAENSYDFKEAKLMVNDAQEVAFNGPVKIQSDNSIAIDIHYTTNELDVEKITALVPKVYSAPLKDLVAKGYAQVSGSVTGVYSDTNTPLVTTNLKYYKGEIKYEAYPRVKAIQLSMSAKVDVNKNQTSNITVTSSSAKILNSTVSVTGNISDIMETLQYDLYSKGDIHLADFQSFIPKDQNVIVRGLVNGNLHTRFSQSDLDQEKYHRIYLTGDFNTRNFDAIYNDSIKINLPSAKIKLNLPSQARADKNLLFANIKIDTPNFTTSLSPTMSAAAQNIELSVDINNLAKGASSPISSCNFKLGSFYAVADTLSVSANNANGRFEYTPSLNGKQEIALINSTMNSDKISIAGKDSILFDAKNLYAKTNLKYDDSQNKVLLKWQPEMAINFSDAIYNLSEQLKGEIPNISFTLNQDSMEIKKADLILGNSDFSLTGQLTDISKYLNDQALLKGNFNLVSKNTDVYQLMDIFNGMGSQDSTLAANKAVASEDDPFMVPKGIEIRLKTTIAMAVVNNHIIENIKGGLTVKDGTLILDQMGFTNKAANMQLTAMYRSDRTNHLFSGIDFHLLNIDVAELIALVPSVNTMIPMLKSFKGKGEFHLAGETYLKSDYSLKQSTIRGAAAFQGQKLTLMDTETFGMIAKKLLFKKKTVNVIDSLSVEMTLFKDEVDLYPFLIVMDNYKAVISGRHNMDNSFNYHISVTDTPLPIRLGLNVTGTIDNLKYDLVECKYKYLYNPKKQGALEKQTLRLKKLISESLKENVKEYE
>NZ_AP018042.1|WP_096431414.1|4425107_4425476_-|hypothetical-protein
MKRIALIFLSIALVALFSTSCTEEEDIHPSFDQTLIIGKWKSGTLFERYDSNKSGATWDTFDDVTEDEAQEFTWTITKDQLEQIHIIVNGGLVPKTYTITDLTASSLKYEDAYGNTKSFSKL
>NZ_AP018042.1|WP_096431416.1|4426462_4427854_+|MFS-transporter
MMMEPNRNLNKTILLAVTMASNFLNPLMGAAVNVALPRIGAEFSMSAVGLSWVSMSFLLASSVFLVPFGKISDNWGRVKMFLYSNVLFGFATLFCAFSFSAETLLIGRLLQGIGAAGIFASNMAIVISAFEPHERGKMIGLNVMAVYVGLSIAPVLGGIITESMGWRSLFFLNAGAVLLIILAVITKIKAEWAVPNSAKFDFKGCLLYMFSVSGLMYGFSHLPKTEAILFTLAGIIGLLIFVRYELKHPNPVFEMQLFFKNKLFAYSNLSAFINYAATFAITFVFSLYLQYVKGLSPKEAGFILIAQPIVMAVVASFSGKLSDTKDPRILASLGMGISAAGLLMLCFISKETPYAYIVFALVILGFGFGLFSSPNTNSIMSSVEKRFIGLASATVATMRTTGMMFSMAVAALSVHVFLGDAKIHAGNLDSFMLSMRVIVIAFTFLCVIGIFTSLVGRRKATDK
>NZ_AP018042.1|WP_096431418.1|4428469_4429657_+|hypothetical-protein
MRLKSCPYIVLLFLIVCFSACSNKTNSKKSELHISLIDSTNHSARLVSVQIFNAHSNYFAYVAGETKSTEVYTINEQGKLDFLEQHQVASKAGGIRALTNVKINDSNLLILGNKADNSIEVYRIENNGKLEKINSVFDTDSTFIDETVTIHDITINNRTFIYAGGLDKGLSCFELSENGALRPIQSIEDNDTLFLHGIIGMSSLVIDNKTFLITGAFFDGGISCFQIMENGHLKNVSNLKDDKNMFLNGTFPVNTVQLGDQNFLLVGHRHKIHYSVDNAEEDYHGDGINVFKVDAQGQIKLHSLLKDNEELLLKGSTRIEILKSDENSAFVFIGTRDDKGIQICSLQQDGILKPIKTIDLGYSIYNGMTLKNMYDNWYLLVGSYDKNKLEMYSIK
>NZ_AP018042.1|WP_145957667.1|4429687_4430863_-|helix-turn-helix-domain-containing-protein
MIYEKDQKAIENALKRICSHPLFANSSIYTRLLEYLVEKALSGEELKEFTIGTDLFGKNYLDDKNDGTVRSYMYKLRKKLAAYYAEADIRESVIFEIKKGQYNLSFIPSNKYHQLKKNTDISIKIPLKALKLAGIIAFTVLAASLVIINQMNTPSNIWKAFFEKMATNMVIVSDQYIVDEIYSDGEMHGVAYPEINNADDFFTYTQKHPEKHLKPNDYTLLSKMAPYSVKRLTQWFDSNKTDYTLQLESELSYDDVRENNIVFVGQFKTMNLSKSLFLKNSKAFTTYLDGFKYETKDTVKVFDTKYGENGKIEYAMVSYTSISPSKKAIYFVSNNDIGVMATLRKFTDKKWLSKFCDGLTKDTNHFNALFEVSGLQRTDISCKLIELEIIE
>NZ_AP018042.1|WP_096433733.1|4431109_4433965_+|DUF4982-domain-containing-protein
MNYKILTIFILLSSAVSGANLPTGYPATSRTKQNINLDWKFSKMPKAEIPAAINFDDSGWKMVCIPHNPDPLSLSLDSVTEHWSQKEFLRDHNWYRKQLYIELEANEKAFIEFEGVHNATELWVNGKLVGRYAVNGYIPHHFDITDFVNSGEVNTIAIDADNSFDATIAPDPDETDYVKWAGIYRDVYLVKTNKLHVNFNWEDFDAGVHITTPSVKKENGTVSVKTTVLNEYEKDADCRIETMIVDADGYVLKKIESSSKIKGGHKNTFRQTTEITDNYHLWSPDNPYLYRAVSVIYLNDKPVDFVENKFGFRSIELVDGQGLLLNGKPFFMLGVNRHQNYPHIGDAVPNSMHYEAALRYKQAGINAIRLSHYPQDNSFIEACDELGIVLYEEASSWIQWKQGEWMNKLEQSLRVMVRNHRNHPSIIIWGAGINHRGPVPQLNNAAKEEDPFRLTASASSPWCGTKNAGVTDIYATMDYRRTDWPEGDFCLLMEHGYSNNSLANQHHISRYKKRNNNIGALLWVGADYNHMRPTEKQHDMFTEYGLQTAYRVPRPAYYWYVSELTDAPYVHITDQSVYKNQKIHVYSNASEVALYANDKLIAVQRADNDPLRIRNAHPSFTFHYNWTQEKLTAKALNRGKVEATHFRTKAGEAYALKLAVDNPDIPLESGGSDLKMIRAYVVDKNGEVITDADLKVHFEATGAGTVFYADKPYVQNMQTYHGAASIYLKGNATAGEVKIKASLGKLKTASLALHTIPYQSDEIANYAAAIYDYPIYKIDIQTEGQLSQFGWRVEKGGDKDEFSFQTGKCTFSVQADSNLKWRKGTNTILGNLGYMACDGVYVESGKLKLILSGLPKGKYVLKTYHHSFQDVKKLFPYNLKVERKDAKGEFTYTSDDEAVGMYDANDIGERKPISVTNYIDSDGVNSVTITFETDKENAATWLNGLEFKQIK
>NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein
MRILKTIAIALLLFPNSINAQQKLDVKLWAVQLEQGKASPLYEVKTDASIDKVEWKNHIFIQPEVTFQKIEGIGGAFNEIGGQALLSLNKEQQQEVMHHLFSNDDAGFTFCRTAIGASDFGLDAYSYSELADDYKMEHFSIDRDNKYVLPYIKSALEVNPNLSLFASPWSPPGWMKESGEMEGLRGKPNKLINEPKVYKAYAKYFVKYLQEYEDQGVRINRICIQNENDADTKYPSCVFPAKEMLSFANNYLFPAFEKNKITTKVYAGTFRAVNKMDLLDFAKCQNTQKIEGVGIQYTDSRFIADAKALQPKLKIFHTEGHCYNGENSAEQAMHRLEEVAGYINSGSTTFTYWNMILNETTKSGWDWPQNSLININRETGDVQYNPDFNAMYIISRFIKPGDVRIASVTRGKYPMISVKSPDGSIKVVIQNTNTEEKTFEILIENQEVKVNIPANTITAVVIKEE

Crispr_ID: NZ_AP018042_9

• CRISPR_ID: NZ_AP018042_9
• CRISPR_location: 4451094-4451286
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 2 spacers
• Cas_protein_info: WYL

Consensus repeat of NZ_AP018042_9

Consensus_repeat	Method
GTATCCTGAGCCGAAGCAAGTCATC	CRISPRCasFinder

spacers of NZ_AP018042_9

>9.1|4451119|59|NZ_AP018042|CRISPRCasFinder
GAATGACTGACGAAAGCTTCCCAATGAGAAAAGAGAGTTATCGAATGAAAAAACAAAGA
>9.2|4451203|59|NZ_AP018042|CRISPRCasFinder
AAATGATGGAACCAAGTTGTTCCTGAACCGAACGAAGCTTCCAAATGAAACAACAAAGT

CRISPR arrays and Neighbor proteins around NZ_AP018042_9

CRISPR arrays

The CRISPR arrays of NZ_AP018042_9

>merge|NZ_AP018042|9|4451094-4451286|CRISPRCasFinder
GTATCCTGAGCCGAAGCAAGTCATCGAATGACTGACGAAAGCTTCCCAATGAGAAAAGAGAGTTATCGAATGAAAAAACAAAGAGTATCCTGAACCGAAGCAAGTCATCAAATGATGGAACCAAGTTGTTCCTGAACCGAACGAAGCTTCCAAATGAAACAACAAAGTCGTTCCTGAACCGAAGCAAATCATT

>NZ_AP018042|9|8|4451094-4451286|CRISPRCasFinder
GTATCCTGAGCCGAAGCAAGTCATC	GAATGACTGACGAAAGCTTCCCAATGAGAAAAGAGAGTTATCGAATGAAAAAACAAAGA
GTATCCTGAACCGAAGCAAGTCATC	AAATGATGGAACCAAGTTGTTCCTGAACCGAACGAAGCTTCCAAATGAAACAACAAAGT
CGTTCCTGAACCGAAGCAAATCATT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096431430.1|4447796_4448792_+|WYL-domain-containing-protein	WYL	pfam13280_WYL_CAS-I:CAS-III	gnl|CDD|379100
NZ_AP018042.1|WP_096431440.1|4453596_4453863_+|transporter-suffix-domain-containing-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431434.1|4449164_4450340_+|ThiF-family-adenylyltransferase	unknown	unknown	gnl|CDD|235717
NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein	unknown	unknown	gnl|CDD|380416
NZ_AP018042.1|WP_096431446.1|4455147_4455378_+|nitrate/nitrite-transporter-NrtS	unknown	unknown	unknown
NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein	unknown	unknown	gnl|CDD|377274
NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1	unknown	unknown	gnl|CDD|238089
NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein	unknown	unknown	gnl|CDD|378982
NZ_AP018042.1|WP_096431438.1|4451822_4452398_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431424.1|4435825_4438435_+|response-regulator	unknown	unknown	gnl|CDD|274362
NZ_AP018042.1|WP_096431426.1|4441012_4441453_+|IS200/IS605-family-transposase	unknown	unknown	gnl|CDD|376616
NZ_AP018042.1|WP_096431444.1|4454888_4455158_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431436.1|4450326_4450728_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_162845481.1|4438743_4440000_+|cadherin-like-domain-containing-protein	unknown	unknown	gnl|CDD|185687
NZ_AP018042.1|WP_096431428.1|4445005_4447672_+|AAA-family-ATPase	unknown	unknown	gnl|CDD|213987
NZ_AP018042.1|WP_096433737.1|4442469_4443867_+|class-II-fumarate-hydratase	unknown	unknown	gnl|CDD|234779
NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein	unknown	unknown	gnl|CDD|374374
NZ_AP018042.1|WP_096431442.1|4454332_4454884_+|sigma-70-family-RNA-polymerase-sigma-factor	unknown	unknown	gnl|CDD|274359
NZ_AP018042.1|WP_162845482.1|4448903_4449161_+|multiubiquitin-domain-containing-protein	unknown	unknown	gnl|CDD|373076
NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein	unknown	unknown	gnl|CDD|227807

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_AP018042.1|WP_096431430.1|4447796_4448792_+|WYL-domain-containing-protein	WYL	pfam13280_WYL_CAS-I:CAS-III	4e-35	116.1	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096431430.1|4447796_4448792_+|WYL-domain-containing-protein	gnl|CDD|379100	pfam13280, WYL, WYL domain. WYL is a Sm-like SH3 beta-barrel fold containing domain. It is a member of the WYL-like superfamily, named for three conserved amino acids found in a subset of the superfamily. However, these residues are not strongly conserved throughout the family. Rather, the conservation pattern includes four basic residues and a position often occupied by a cysteine, which are predicted to line a ligand-binding groove typical of the Sm-like SH3 beta-barrels. A WYL domain protein (sll7009) is a negative regulator of the I-D CRISPR-Cas system in Synechocystis sp. It is predicted to be a ligand-sensing domain that could bind negatively charged ligands, such as nucleotides or nucleic acid fragments, to regulate CRISPR-Cas and other defense systems such as the abortive infection AbiG system.	1.76712e-30
NZ_AP018042.1|WP_096431434.1|4449164_4450340_+|ThiF-family-adenylyltransferase	gnl|CDD|235717	PRK06153, PRK06153, hypothetical protein; Provisional.	6.03408e-178
NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein	gnl|CDD|380416	cd10548, cupin_CDO, cysteine dioxygenase, cupin domain. This family contains cysteine dioxygenase (CDO; EC 1.13.11.20), which catalyzes the conversion of cysteine to cysteine sulfinic acid, the first step in the biosynthesis of essential oxidized cysteine metabolites such as sulfate, hypotaurine, and taurine. CDO also plays an important role in the regulation of intracellular cysteine levels in mammals; CDO expression is altered in cancer cells, and abnormal or deficient CDO activity has been linked to Parkinson's disease, Alzheimer's disease, and rheumatoid arthritis. CDO is an iron-dependent thiol dioxygenase that uses molecular oxygen to oxidize the sulfhydryl group of cysteine to generate cysteine sulfinic acid. The CDO active site contains an amino acid-derived cofactor. These enzymes are found in prokaryotes as well as eukaryotes and belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.	1.2743e-22
NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein	gnl|CDD|377274	pfam04264, YceI, YceI-like domain. E. coli YceI is a base-induced periplasmic protein. The recent structure of a member of this family shows that it binds to poly-isoprenoid. The structure consists of an extended, eight-stranded, antiparallel beta-barrel that resembles the lipocalin fold.	8.46698e-17
NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1	gnl|CDD|238089	cd00158, RHOD, Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and certain stress proteins such as senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions (no active site cysteine) are also seen in dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases, where they are believed to play a regulatory role in multidomain proteins.	2.67611e-28
NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein	gnl|CDD|378982	pfam12869, tRNA_anti-like, tRNA_anti-like. This is a family of bacterial, archeael and viral proteins that is related to the tRNA_anti family pfam01336. The major characteristic of families like tRNA_anti is their OB-fold, and many of them bind DNA.	1.47327e-06
NZ_AP018042.1|WP_096431424.1|4435825_4438435_+|response-regulator	gnl|CDD|274362	TIGR02956, sensor_protein_TorS, TMAO reductase sytem sensor TorS. This protein, TorS, is part of a regulatory system for the torCAD operon that encodes the pterin molybdenum cofactor-containing enzyme trimethylamine-N-oxide (TMAO) reductase (TorA), a cognate chaperone (TorD), and a penta-haem cytochrome (TorC). TorS works together with the inducer-binding protein TorT and the response regulator TorR. TorS contains histidine kinase ATPase (pfam02518), HAMP (pfam00672), phosphoacceptor (pfam00512), and phosphotransfer (pfam01627) domains and a response regulator receiver domain (pfam00072). [Signal transduction, Two-component systems].	5.00831e-58
NZ_AP018042.1|WP_096431426.1|4441012_4441453_+|IS200/IS605-family-transposase	gnl|CDD|376616	pfam01797, Y1_Tnp, Transposase IS200 like. Transposases are needed for efficient transposition of the insertion sequence or transposon DNA. This family includes transposases for IS200 from E. coli.	9.08752e-28
NZ_AP018042.1|WP_162845481.1|4438743_4440000_+|cadherin-like-domain-containing-protein	gnl|CDD|185687	cd02178, GH16_beta_agarase, Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.	2.44831e-38
NZ_AP018042.1|WP_096431428.1|4445005_4447672_+|AAA-family-ATPase	gnl|CDD|213987	cd07432, PHP_HisPPase, Polymerase and Histidinol Phosphatase domain of Histidinol phosphate phosphatase. HisPPase catalyzes the eighth step of histidine biosynthesis, in which L-histidinol phosphate undergoes dephosphorylation to produce histidinol. HisPPase can be classified into two types: the bifunctional HisPPase found in proteobacteria that belongs to the DDDD superfamily and the monofunctional Bacillus subtilis type that is a member of the PHP family. The PHP (also called histidinol phosphatase-2/HIS2) domain is associated with several types of DNA polymerases, such as PolIIIA and family X DNA polymerases, stand alone histidinol phosphate phosphatases (HisPPases), and a number of uncharacterized protein families. The PHP domain has four conserved sequence motifs and contains an invariant histidine that is involved in metal ion coordination. The PHP domain of HisPPase is structurally homologous to other members of the PHP family that have a distorted (beta/alpha)7 barrel fold with a trinuclear metal site on the C-terminal side of the barrel.	5.06897e-21
NZ_AP018042.1|WP_096433737.1|4442469_4443867_+|class-II-fumarate-hydratase	gnl|CDD|234779	PRK00485, fumC, fumarate hydratase; Reviewed.	0
NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein	gnl|CDD|374374	pfam16117, DUF4833, Domain of unknown function (DUF4833). This family consists of uncharacterized proteins around 170 residues in length and is mainly found in various Parabacteroides and Bacteroides species. The function of this family is unknown.	7.44201e-41
NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein	gnl|CDD|227807	COG5520, COG5520, O-Glycosyl hydrolase [Cell envelope biogenesis, outer membrane].	1.06334e-40
NZ_AP018042.1|WP_162845482.1|4448903_4449161_+|multiubiquitin-domain-containing-protein	gnl|CDD|373076	pfam14452, Multi_ubiq, Multiubiquitin. A ubiquitin superfamily domain that is often present in multiple tandem copies in the same polypeptide. Members of this family are associated in gene neighborhoods, or on occasions fused to, bacterial homologs of components of ubiquitin-dependent modification system such as the E1, E2 and JAB metallopeptidase enzymes and a distinct metal-binding domain. The E2/UBC fold domain appears to be inactive. The JAB domain in these operons is usually fused to the E1 domain.	2.32159e-14
NZ_AP018042.1|WP_096431442.1|4454332_4454884_+|sigma-70-family-RNA-polymerase-sigma-factor	gnl|CDD|274359	TIGR02943, RNA_polymerase_sigma-24_subunit_RpoE, RNA polymerase sigma-70 factor, TIGR02943 family. This group of sigma factors are members of the sigma-70 family (TIGR02937). They and appear by homology, tree building, bidirectional best hits and one-to-a-genome distribution, to represent a conserved family.	5.3003e-42

Protein sequences

>NZ_AP018042.1|WP_096431436.1|4450326_4450728_+|hypothetical-protein
MERIKLLKVHYLPKELEEGFLYVSKEFGVAGHLCPCGCKNKIITPLDLAEWSFKEVNNKPTLYPSIGNWQLPCKSHYWIIDGKIVWSYQWSEEQIKEGGEAEEEKRKSYYYNLENKYNKQSLFKRFIKFVFGK
>NZ_AP018042.1|WP_096431434.1|4449164_4450340_+|ThiF-family-adenylyltransferase
MATKLAKYNEDIDRLLKKGYALSIDSNHLVVRDIPYLDNKGELNIGAIVSILNSIDQYKVGMVDHQIYFCGSHPHELSGKPIRNLGGGPVQLKLTSDDLIVQRSFSNKPPSGKFENWFDKIESYVTIISGPSMNKFDIHPYTFRVVAENSNSVFKFRDTLSSRSEIGDLSAKLEEDTVAIIGLGGTGAYLLDFLVKTPVKEIRGFDLDWYHVHNAFRSPGKLEADELGKRKSEVYKQRYEGFRDNINIHSNYITSDSEKDLVGVTFAFVCVDSGTSRAEIFELLIKLKIPFIDIGMGLDRNMGAISGMLRTTSFSKESAQNLMEKRLAPLSDIPDDVYKNNIQISELNALNACLAIIKYKQLREFYVDDNSYYHTLFNIDGLNCFGENGKN
>NZ_AP018042.1|WP_162845482.1|4448903_4449161_+|multiubiquitin-domain-containing-protein
MSNSKEKKEVTIFINGEPHEVEKDEITYDEVVTLAFPDFPQHPERTYSVTYERGQGNKPTGILSPGGTVKVKEGMRFKVKHTGQS
>NZ_AP018042.1|WP_096431430.1|4447796_4448792_+|WYL-domain-containing-protein
MPKNKEALKRYRIIHNILRRGGRHKTRRIVEICNDAGIPVKLRTIQTDLKDLAEDTELGFYLPIVKDERTKTYFYSEIPENIFPSLELEPEEITALLFYAKTVSQYKEYPIFKEITNAVQKVFDNSNIDPELKELFEKETLLETEKHPQIGGIELISDILEAISKKKIIEIEYHKFDDVIKCHRLKPILLKEDKQLWYILGINVKHDRLITFALDRIIDVTITEDLFDEVPFDSKEYFKYSFGITVTEEDPIDVIISFNPEQGNYLKTLPIHLSQIVLEDTTSKFVIKVSVKPSYEFYSKIFSYGSSATVLAPESICKIFKQTFVEALSRY
>NZ_AP018042.1|WP_096431428.1|4445005_4447672_+|AAA-family-ATPase
MIDYTTTNSGAKFYKADLHIHSYGEDGSFDVTDTTMTPENIVDISIEKGLSIIAITDHNEVQNSKKAIKHAEGKEILVIPGIEISTLQGHLLVYFEKHEDLKNFRGKLTISDDRETCTQGIVECLNFAKQYNGLGILAHISSNAGFEKTIGRFDPKMSKILEHECLYGLEISDKSEIDLYTERDKSEDGQKRMHFVNQRIDFLDSEKDFEFPKLMSSDAHTTDRLGTNADGEKKLTRIKLDIISFHAFRIALISSNSRVRIENLIPEKLPRFVGVSLNGGLLANQIIHFSSNLTCIIGGRGAGKSTLLEALRVGSGNNSEMSVVDSEVWPDEINLTYEDETGQLISFVREKNSYPLNSSTEEGLSKIPIESYGQGETAKTIQFSEQNPQYLIDFLNSFLDLDELNSDDRTLRDKLHENQSNLRGLRLAVTQIPEVTKMKVNLEGKLKQLQQDKASELVNFHLALVREKEIRTDIKEDLNELFTKYRDILSDKTIFDSFEEIDGNEIKVGKDNFIKVKEIVKMFGDIVSVKQIELSTELKQKIEELKVQLQDWASKETEIQAKINAKKKELEEKGIPFDIGKINQITKDVVTYQNKLKLLKQKEVELKDLLKSRDVLIRQRLDVKSKIYYQHYEFGKTINDNLKNSVDDFFVSVKYKEGKYSPSFEQAIKNLAQWRLNKKSEAFANSITPLEFVMAIRKKNLDFMKDIEDQNGRMFNDEEINSLLSKAQENYNYEDFDTLDYDDRPSIIVTKTVSDENGTMQTVRRSLNQLSLGQQQSILLAILLQSKSKVPLLIDQPEDNLDSEFIYKSIVKNLRRIKEFRQVIIVTHNPNIAVLGDAELIIPLKSTSIKSVIMERGSIDTEQTRKSCCEILEGGKQAFKRRQEIYKI
>NZ_AP018042.1|WP_096433737.1|4442469_4443867_+|class-II-fumarate-hydratase
MEYRVVKDTMGEVKVPVNCYWGAQTERSRNNFKIGHEASMPKEIIHAFAYLKKAAAITNHQLAVLVEDKKELIAKVCDEIIAGKHDKEFPLVIWQTGSGTQSNMNINEVIANRAHVIQGNRLGEGKLYVHPNDDVNKSQSSNDTFPTAMHIAAYKMVVENTIPGIETLADTLFKKSQDFKGVVKIGRTHFMDATPLSLGQEFSGYSQQLTNSIKAIKNALKTVSELAMGGTAVGTGLNTPKGYDVMVAQKIAELTGLPFITAPNKFEAIAAHDGMVELSGALKRCAVSLMKIGNDIRMLSSGPRCGIGELIIPENEPGSSIMPGKVNPTQTEALTMVCAQVMGNDVAVSIGGSNGHFELNAFQPLIAANVLQSARLIGDACASFNKNCIVGILPNFPILKQYLEKSLMLVTALNTHIGYENAAIIVKKAHVENKTLREAALELNLVTNEQFDEWVKPELMCGSME
>NZ_AP018042.1|WP_096431426.1|4441012_4441453_+|IS200/IS605-family-transposase
MSWVRVYVHMVFSTKNREPFLNSSELKKNVFQHIKKNAEEKEIWLDCVNGHQDHVHCLISLGKEQSISKVAQLIKGESSFWINQQKLMAERFIWQDDYWVVGVSESHLESVRKYIHNQEEHHSKQSFTDEINVFMEKYGWSFIKGK
>NZ_AP018042.1|WP_162845481.1|4438743_4440000_+|cadherin-like-domain-containing-protein
MSLLVILSLFIGISSCSSNSEPDTVPEVENDNPTAVNDNLRVDEDSSAGTLNQIIVSVNDKIGEDGGDGDDYSLLSDAGNGTVSEISDGVFEYVPNADFNGTDSFSYAITDRDGDSDTGTVTITVNAVADGPTAEDFNNIDPNYPSFVSIDDTTPEGKQWVKMASMSDEFEAWDADKWFKSTWNYGVPVYMSTSDINSGVADGNLWIKATLNESNPEGRWFQTARIHSKAETTYPMYTESRIKAAYISAFNTYWLNNGNSTNRDEIDIIENNSKPSCDCQPDYPTQMNSQYFHADSDKTPVEIRDEDNFNQTNLSDANPGKGLKWNEDYHTFGVWWKDSKNIQFYLDGELAGSVLVGHDRSGTTYSEREFTRELEIIFDLWTADYDWLGGLALKADLADNSINTMKIDWVRTWKLEDK
>NZ_AP018042.1|WP_096431424.1|4435825_4438435_+|response-regulator
MNTRKFKTNKIISINRYFFVPIALILFFLTFYMIYEDIKSNTINEFNNEQLILAETASQGITSFFDYYESELVFIAQLKEIIDFSEESKAFMANYYETHKSIVTAVTRIDTTGVILYTYPYNQSVIGQDISYQDHIQQVIIDHKPVLSDVFMSAQGFLAIAMHVPVFKNKNYVGSLSVLIPIDRLGKLYLEKIKNRKKGHAWLLSENGIEIYCPISGHTGKSILETTKHDASSVEMLETINNNSTGTGKSIHQEVFDNDKTKLLDLYYAFYRVPLGNTYWTILISYQEEDVYIALSRLRNRLIFVFSLLFIALSFYFYSLTKVRNFLKEEAKRKEVEKILFKSEEKFRKIFDDHAAVKLILDPNTGNIVDANKSAANYYGWSCEELIKMNIGQLNGLSAQEITKNIENILSKNKNQFEFKHQLKDGSIRDVEVLSSTINIDNNDVLHSIIYDITDRKNAEVLLKEKTDKIASQNEEYLCVNEELNQRNQELIIAKEKAEESDHLKTSFLQNISHEIRTPMNAVVGFSSFLNDPDLLPEKRKYFTDIIIQSSDQLLSIINDIVSIASIESGHEKVQGNEININLICKLINEQFFLKDTKPNVTCSLQTSLADDEAIIITDSTKLTQILTNLIGNALKFTKKGFVNFGYTLKDDFLEFYVEDSGIGIPSDMHAEIFKRFRQAEKTTARMFGGSGLGLSISKAYVEMLGGTIWLNSEPGKGSVFYFTLPYKKVNSKTISKLPVNELNLGFTKSKTVLIAEDEDYNFILLEQYLSDMNITIIRAVNGIEAVAICKSNPQVDLVLMDMKMPEMDGFEATKRIKEFRPNLAIIAQSAYSSKADKNKAFACGCSDFISKPFKREMLLSKIKEQLLK
>NZ_AP018042.1|WP_096431422.1|4433984_4435382_+|hypothetical-protein
MRILKTIAIALLLFPNSINAQQKLDVKLWAVQLEQGKASPLYEVKTDASIDKVEWKNHIFIQPEVTFQKIEGIGGAFNEIGGQALLSLNKEQQQEVMHHLFSNDDAGFTFCRTAIGASDFGLDAYSYSELADDYKMEHFSIDRDNKYVLPYIKSALEVNPNLSLFASPWSPPGWMKESGEMEGLRGKPNKLINEPKVYKAYAKYFVKYLQEYEDQGVRINRICIQNENDADTKYPSCVFPAKEMLSFANNYLFPAFEKNKITTKVYAGTFRAVNKMDLLDFAKCQNTQKIEGVGIQYTDSRFIADAKALQPKLKIFHTEGHCYNGENSAEQAMHRLEEVAGYINSGSTTFTYWNMILNETTKSGWDWPQNSLININRETGDVQYNPDFNAMYIISRFIKPGDVRIASVTRGKYPMISVKSPDGSIKVVIQNTNTEEKTFEILIENQEVKVNIPANTITAVVIKEE
>NZ_AP018042.1|WP_096431438.1|4451822_4452398_+|hypothetical-protein
MKNKVAITFLLILLIGSSINGYSQNVVAQRLAVSERSIPKIVSNTFKKQYPDILLKGWYVTHLTYWQNDYSSDWYNGWYGQRTVVVYTYQKPNYFEVEFVNNPGELSRAIYNINGYWYETRTQIKGLTMGIHNALKESKFNAWKISTTKEKLESPAWPIEIYRFGVSKGLQSQIISIDAEGNFIQMKELKK
>NZ_AP018042.1|WP_096431440.1|4453596_4453863_+|transporter-suffix-domain-containing-protein
MKKKNWRIRVGILLIVFCIPFFLFLPAIPFLEMEAKSKITLSTISLIIGEVMFWVGGILVGKELFVKYKSYFNPKNWFKKGDKNKESQ
>NZ_AP018042.1|WP_096431442.1|4454332_4454884_+|sigma-70-family-RNA-polymerase-sigma-factor
MENENLIKWVNEFTNELYKWAYYKTSSAETAEDLVQETFLAAAEKVSTFKGDSSAKTWLFSILNHKIIDYYRKKVNKPVAMESNTIASYFDEDGSWQKEKRPKDWQDEENHLLDDNDFQQILKKCLDALPEKWSTCVKLKYLTEKSGEDICQELEIAPTNYWQIVHRSKLQLRNCIENNWFKN
>NZ_AP018042.1|WP_096431444.1|4454888_4455158_+|hypothetical-protein
MKKLMHLFFLSCLKATELIEKKFHFELSVKEKLQLKMHKMMCSACSNYEKQSSLIEKVISNLEKSKVSTIDVEALKSTITKKIEGLDEN
>NZ_AP018042.1|WP_096431446.1|4455147_4455378_+|nitrate/nitrite-transporter-NrtS
MKTKNVSYLQLATDKTSLFRALKVALFIGIILNLINNPQLFQVSSDTEIHLSRIILTFLVPFCVSLFSSVLANRNK
>NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1
MKRILSLVFPCFLFLTACGQNQTIKEVNSTEFEKLIKDSSGTLLDVRTLGEFKNGHIKDAGQLNYYALGFRKKLLLLPKNQAIYLYCNTGYRSQKAAEILAENGYKNIYNLEHGIMEWNLKNLPVLVDLDAKPDTENKMEVDEFTALINSEQLVFADFYAPWCAPCRKMMPMIDSLKTEYKDKITIVKINSDASKKLMKELTVVSVPYLVLYRKGKIIFTHNGSIGKNELVSLFEENRMKYTQVQGDEK
>NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein
MIPKKKKILKIGVILLIAGLLIGGGTVLYMFNMPHRNVQSASTDFSLSASQLVAEYLENPAESNEKYLANDGESKVLEIKGTVAKISEDFKGQKVVLLKGTSDKAGVSCTFTKETNENISNTRVGEIVTIKGVIRLGASFDSDLEMYENVVLEKSDLVK
>NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein
MKKVKFLVSMSAIVLTLFAFSPPVGKLVSSKTHVKFFSHTGVEDIQANNYTSISTIAPSTGDVVFSVPMQSFEFEKALMQKHFNSKKFLETKAFPKAKLKGKITNLAQINFEKDGMYLANFEGNLTIKGVTKPIKEKGNITVKGNQIEVQSTFNITLADYGITFVKGKPASNIAKTVEITVHAEYQAK
>NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein
MIKFIFKSVLLLSFGLLSTGLRVAPEDKILFSIGRSKDANEIYYSLNLDKYNRLNAENPIQVYWIKRTRKSKIVPLTWIQQKFAYGIVYLSKSENYAKFRLAAYDKRVFELKKNKNNEFRVFTMSNNVEVEVDRVFIYITGGTFWIPKIPKVELYATLSRTDKKIKETIIP
>NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein
MNTGFSKNIENLINDLRNQNSVTNPMVLEMVESYKLTEQDVAQYFSYNHSPDESYGRQLIYDNGNFKILLMSWKPGDFTAIHNHGYTEWGCVYFFGEATHRLYSVTNDELKIIQKDNFQKGQIASVSGNLTHMMGNSSSKNFSTLHIYGSNSQQSDVSKDAIVYLPELDKEVTTMGSAYLNMDKQLILSEKPLINVSNELVADYFSLVKLFYECNGLSPLLRNMEKKLGNRSIN

Crispr_ID: NZ_AP018042_10

• CRISPR_ID: NZ_AP018042_10
• CRISPR_location: 4465477-4465614
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_10

Consensus_repeat	Method
TTTTCCTTTGTGTGTCTTTGTGATTTCCTTATGTGACCTTAGTGGTTA	CRISPRCasFinder

spacers of NZ_AP018042_10

>10.1|4465525|42|NZ_AP018042|CRISPRCasFinder
GTTTTTTTTGGTTGTGGATAAAGTGAGTAACCACAAAGAGCT

CRISPR arrays and Neighbor proteins around NZ_AP018042_10

CRISPR arrays

The CRISPR arrays of NZ_AP018042_10

>merge|NZ_AP018042|10|4465477-4465614|CRISPRCasFinder
TTTTCCTTTGTGTGTCTTTGTGATTTCCTTATGTGACCTTAGTGGTTAGTTTTTTTTGGTTGTGGATAAAGTGAGTAACCACAAAGAGCTTTTTCCTTTGTGTGTCTTTGTGATTTCCTTATGTGTCCTTAGTGGTTA

>NZ_AP018042|10|9|4465477-4465614|CRISPRCasFinder
TTTTCCTTTGTGTGTCTTTGTGATTTCCTTATGTGACCTTAGTGGTTA	GTTTTTTTTGGTTGTGGATAAAGTGAGTAACCACAAAGAGCT
TTTTCCTTTGTGTGTCTTTGTGATTTCCTTATGTGTCCTTAGTGGTTA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096431475.1|4470146_4470890_-|sulfite-exporter-TauE/SafE-family-protein	unknown	unknown	gnl|CDD|376667
NZ_AP018042.1|WP_096431483.1|4473528_4473924_-|response-regulator	unknown	unknown	gnl|CDD|381099
NZ_AP018042.1|WP_096431471.1|4466475_4467321_+|patatin-family-protein	unknown	unknown	gnl|CDD|132847
NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein	unknown	unknown	gnl|CDD|380416
NZ_AP018042.1|WP_096431485.1|4474132_4474759_-|chloramphenicol-acetyltransferase	unknown	unknown	gnl|CDD|215002
NZ_AP018042.1|WP_096431473.1|4467507_4469967_+|TonB-dependent-receptor	unknown	unknown	gnl|CDD|379720
NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1	unknown	unknown	gnl|CDD|238089
NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein	unknown	unknown	gnl|CDD|378982
NZ_AP018042.1|WP_096431465.1|4463726_4464353_+|class-I-SAM-dependent-methyltransferase	unknown	unknown	gnl|CDD|379312
NZ_AP018042.1|WP_096431477.1|4470990_4471596_-|MarC-family-protein	unknown	unknown	gnl|CDD|225006
NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein	unknown	unknown	gnl|CDD|377274
NZ_AP018042.1|WP_096431463.1|4461769_4463185_+|sodium:solute-symporter-family-protein	unknown	unknown	gnl|CDD|271357
NZ_AP018042.1|WP_096431481.1|4472434_4473316_-|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|223669
NZ_AP018042.1|WP_145957668.1|4460302_4461277_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein	unknown	unknown	gnl|CDD|374374
NZ_AP018042.1|WP_096431467.1|4464577_4465273_+|SIMPL-domain-containing-protein	unknown	unknown	gnl|CDD|377344
NZ_AP018042.1|WP_153244858.1|4474824_4474983_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096431469.1|4465720_4466455_+|1-acyl-sn-glycerol-3-phosphate-acyltransferase	unknown	unknown	gnl|CDD|153251
NZ_AP018042.1|WP_096431479.1|4471924_4472380_-|methionine-R-sulfoxide-reductase	unknown	unknown	gnl|CDD|235499
NZ_AP018042.1|WP_162845483.1|4458906_4460322_+|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	unknown	unknown	gnl|CDD|99743

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096431475.1|4470146_4470890_-|sulfite-exporter-TauE/SafE-family-protein	gnl|CDD|376667	pfam01925, TauE, Sulfite exporter TauE/SafE. This is a family of integral membrane proteins where the alignment appears to contain two duplicated modules of three transmembrane helices. The proteins are involved in the transport of anions across the cytoplasmic membrane during taurine metabolism as an exporter of sulfoacetate. This family used to be known as DUF81.	5.83799e-18
NZ_AP018042.1|WP_096431483.1|4473528_4473924_-|response-regulator	gnl|CDD|381099	cd17546, REC_hyHK_CKI1_RcsC-like, phosphoacceptor receiver (REC) domain of hybrid sensor histidine kinases/response regulators similar to Arabidopsis thaliana CKI1 and Escherichia coli RcsC. This family is composed of hybrid sensor histidine kinases/response regulators that are sensor histidine kinases (HKs) fused with a REC domain, similar to the sensor histidine kinase CKI1 from Arabidopsis thaliana, which is involved in multi-step phosphorelay (MSP) signaling that mediates responses to a variety of important stimuli in plants. MSP involves a signal being transferred from HKs via histidine phosphotransfer proteins (AHP1-AHP5) to nuclear response regulators. The CKI1 REC domain specifically interacts with the downstream signaling protein AHP2, AHP3 and AHP5. The plant MSP system has evolved from the prokaryotic two-component system (TCS), which allows organisms to sense and respond to changes in environmental conditions. This family also includes bacterial hybrid sensor HKs such as Escherichia coli RcsC, which is a component of the Rcs signalling pathway that controls a variety of physiological functions like capsule synthesis, cell division, and motility. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.	1.14473e-38
NZ_AP018042.1|WP_096431471.1|4466475_4467321_+|patatin-family-protein	gnl|CDD|132847	cd07208, Pat_hypo_Ecoli_yjju_like, Hypothetical patatin similar to yjju protein of Escherichia coli. Patatin-like phospholipase similar to yjju protein of Escherichia coli. This family predominantly consists of bacterial patatin glycoproteins, and some representatives from eukaryotes and archaea. The patatin protein accounts for up to 40% of the total soluble protein in potato tubers. Patatin is a storage protein, but it also has the enzymatic activity of a lipid acyl hydrolase, catalyzing the cleavage of fatty acids from membrane lipids. Members of this family have also been found in vertebrates.	4.74428e-94
NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein	gnl|CDD|380416	cd10548, cupin_CDO, cysteine dioxygenase, cupin domain. This family contains cysteine dioxygenase (CDO; EC 1.13.11.20), which catalyzes the conversion of cysteine to cysteine sulfinic acid, the first step in the biosynthesis of essential oxidized cysteine metabolites such as sulfate, hypotaurine, and taurine. CDO also plays an important role in the regulation of intracellular cysteine levels in mammals; CDO expression is altered in cancer cells, and abnormal or deficient CDO activity has been linked to Parkinson's disease, Alzheimer's disease, and rheumatoid arthritis. CDO is an iron-dependent thiol dioxygenase that uses molecular oxygen to oxidize the sulfhydryl group of cysteine to generate cysteine sulfinic acid. The CDO active site contains an amino acid-derived cofactor. These enzymes are found in prokaryotes as well as eukaryotes and belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.	1.2743e-22
NZ_AP018042.1|WP_096431485.1|4474132_4474759_-|chloramphenicol-acetyltransferase	gnl|CDD|215002	smart01059, CAT, Chloramphenicol acetyltransferase. Chloramphenicol acetyltransferase (CAT).catalyzes the acetyl-CoA dependent acetylation of chloramphenicol (Cm), an antibiotic which inhibits prokaryotic peptidyltransferase activity. Acetylation of Cm by CAT inactivates the antibiotic. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism. There is a second family of CAT. evolutionary unrelated to the main family described above. These CAT belong to the bacterial hexapeptide-repeat containing-transferases family (see ). The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilised by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilisation is provided by an unusual interaction with a main-chain carbonyl oxygen.	1.76548e-68
NZ_AP018042.1|WP_096431473.1|4467507_4469967_+|TonB-dependent-receptor	gnl|CDD|379720	pfam14905, OMP_b-brl_3, Outer membrane protein beta-barrel family. This family includes proteins annotated as TonB dependent receptors. But it is also likely to contain other membrane beta barrel proteins of other functions.	2.95086e-89
NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1	gnl|CDD|238089	cd00158, RHOD, Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and certain stress proteins such as senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions (no active site cysteine) are also seen in dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases, where they are believed to play a regulatory role in multidomain proteins.	2.67611e-28
NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein	gnl|CDD|378982	pfam12869, tRNA_anti-like, tRNA_anti-like. This is a family of bacterial, archeael and viral proteins that is related to the tRNA_anti family pfam01336. The major characteristic of families like tRNA_anti is their OB-fold, and many of them bind DNA.	1.47327e-06
NZ_AP018042.1|WP_096431465.1|4463726_4464353_+|class-I-SAM-dependent-methyltransferase	gnl|CDD|379312	pfam13649, Methyltransf_25, Methyltransferase domain. This family appears to be a methyltransferase domain.	4.70636e-13
NZ_AP018042.1|WP_096431477.1|4470990_4471596_-|MarC-family-protein	gnl|CDD|225006	COG2095, MarC, Multiple antibiotic transporter [Intracellular trafficking and secretion].	8.00447e-24
NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein	gnl|CDD|377274	pfam04264, YceI, YceI-like domain. E. coli YceI is a base-induced periplasmic protein. The recent structure of a member of this family shows that it binds to poly-isoprenoid. The structure consists of an extended, eight-stranded, antiparallel beta-barrel that resembles the lipocalin fold.	8.46698e-17
NZ_AP018042.1|WP_096431463.1|4461769_4463185_+|sodium:solute-symporter-family-protein	gnl|CDD|271357	cd10322, SLC5sbd, Solute carrier 5 family, sodium/glucose transporters and related proteins; solute-binding domain. This family represents the solute-binding domain of SLC5 proteins (also called the sodium/glucose cotransporter family or solute sodium symporter family) that co-transport Na+ with sugars, amino acids, inorganic ions or vitamins. Family members include: the human glucose (SGLT1, 2, 4, 5), chiro-inositol (SGLT5), myo-inositol (SMIT), choline (CHT), iodide (NIS), multivitamin (SMVT), and monocarboxylate (SMCT) cotransporters, as well as Vibrio parahaemolyticus glucose/galactose (vSGLT), and Escherichia coli proline (PutP) and pantothenate (PutF) cotransporters. Vibrio parahaemolyticus Na(+)/galactose cotransporter (vSGLT) has 13 transmembrane helices (TMs): TM-1, an inverted topology repeat: TMs1-5 and TMs6-10, and TMs 11-12 (TMs numbered to conform to the solute carrier 6 family Aquifex aeolicus LeuT). One member of this family, human SGLT3, has been characterized as a glucose sensor and not a transporter. Members of this family are important in human physiology and disease.	1.08217e-74
NZ_AP018042.1|WP_096431481.1|4472434_4473316_-|alpha/beta-hydrolase	gnl|CDD|223669	COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].	3.6388e-19
NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein	gnl|CDD|374374	pfam16117, DUF4833, Domain of unknown function (DUF4833). This family consists of uncharacterized proteins around 170 residues in length and is mainly found in various Parabacteroides and Bacteroides species. The function of this family is unknown.	7.44201e-41
NZ_AP018042.1|WP_096431467.1|4464577_4465273_+|SIMPL-domain-containing-protein	gnl|CDD|377344	pfam04402, SIMPL, Protein of unknown function (DUF541). Members of this family have so far been found in bacteria and mouse SwissProt or TrEMBL entries. However possible family members have also been identified in translated rat (Genbank:AW144450) and human (Genbank:AI478629) ESTs. A mouse family member has been named SIMPL (signalling molecule that associates with mouse pelle-like kinase). SIMPL appears to facilitate and/or regulate complex formation between IRAK/mPLK (IL-1 receptor-associated kinase) and IKK (inhibitor of kappa-B kinase) containing complexes, and thus regulate NF-kappa-B activity. Separate experiments demonstrate that a mouse family member (named LaXp180) binds the Listeria monocytogenes surface protein ActA, which is a virulence factor that induces actin polymerization. It may also bind stathmin, a protein involved in signal transduction and in the regulation of microtubule dynamics. In bacteria its function is unknown, but it is thought to be located in the periplasm or outer membrane.	6.52979e-09
NZ_AP018042.1|WP_096431469.1|4465720_4466455_+|1-acyl-sn-glycerol-3-phosphate-acyltransferase	gnl|CDD|153251	cd07989, LPLAT_AGPAT-like, Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: AGPAT-like. Lysophospholipid acyltransferase (LPLAT) superfamily member: acyltransferases of de novo and remodeling pathways of glycerophospholipid biosynthesis which catalyze the incorporation of an acyl group from either acylCoAs or acyl-acyl carrier proteins (acylACPs) into acceptors such as glycerol 3-phosphate, dihydroxyacetone phosphate or lyso-phosphatidic acid. Included in this subgroup are such LPLATs as 1-acyl-sn-glycerol-3-phosphate acyltransferase (AGPAT, PlsC), Tafazzin (product of Barth syndrome gene), and similar proteins.	2.37766e-29
NZ_AP018042.1|WP_096431479.1|4471924_4472380_-|methionine-R-sulfoxide-reductase	gnl|CDD|235499	PRK05550, PRK05550, bifunctional methionine sulfoxide reductase B/A protein; Provisional.	4.90989e-78
NZ_AP018042.1|WP_162845483.1|4458906_4460322_+|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	gnl|CDD|99743	cd06450, DOPA_deC_like, DOPA decarboxylase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD correspond to DOPA/tyrosine decarboxylase (DDC), histidine decarboxylase (HDC), and glutamate decarboxylase (GDC). DDC is active as a dimer and catalyzes the decarboxylation of tyrosine. GDC catalyzes the decarboxylation of glutamate and HDC catalyzes the decarboxylation of histidine.	1.94408e-101

Protein sequences

>NZ_AP018042.1|WP_096431467.1|4464577_4465273_+|SIMPL-domain-containing-protein
MKRNVILLLAVFLAWSAMAQAQKNFIDQNYIEVKGLAELEIVPDEIYLKIHLDEEDTKNKESVEVLEKQMFVALKKAGINLEKQLSVSDFASTLQDHFIKRADMKKSKDFQLLVHDSKTLGKVFVELDRIKISNISILRVDHSEIEKFRKQVKINAVVAAKEKAAALAEAIGQKIGKAIYINEISAPYRGQMVNTMMRTKSESYQSNMAMPELDFQKIILEYSVMISFALE
>NZ_AP018042.1|WP_096431465.1|4463726_4464353_+|class-I-SAM-dependent-methyltransferase
MNNFDRKKHWETIYQSKESKDVSWFQSSPETSLDFFKQFNVAPSAKIIDVGGGDSLLVDHLLDLGYQNISVLDISEAAIEKAKQRLGERAKKVSWIVADAATFKPSEKYDFWHDRAAFHFLTGEQEISNYLDTAQKNIKPEGVLVIGTFSEQGPQKCSGIEIKQYSETSMNDLLTNFFSKIKCITVDHKTPSGVVQNFVFCSFRKLHV
>NZ_AP018042.1|WP_096431463.1|4461769_4463185_+|sodium:solute-symporter-family-protein
MHIIDISIFIIYMLGMLGVGIYFMKRNKSQEDYYVGGREMTSFHIGLSVVATDVGGGFSIGLGGLGFLIGLSGSWMLFTGIIGAWISAIFLIPIIYPLAKKHNFLSFPEVLNHFYDRKVAIIAGIISLIGYIGFTSSQVLAGAKLAAATFPSITIVDAVLLMGVVVIGYTVLGGLKAVIFTDTIQWIILMVGLILIGIPLGFIKVGGWEALRIYLPANFQSLTNVGFVQFINWFITIVPIWFVGMTLYQRIYASKDEKTAKKAWFIAGLFEWPVMAFMGITLGLLGRVAFEQGMFTEFGYAPGSAIDPEIGLPLLLTHIFPVGLMGLLMSSYFSAIMSTADSCLMAASGNFTTDILRLSKDNTKGIRYSQLATLVIGILAIFLATKMQNVLDLMLYSYAFMVSGLFVPVLGFMFLKNPSAKAAMYAMLLGGGTTLALILTELALPFGLDANFFGITLSAIVFTSIQSLHKR
>NZ_AP018042.1|WP_145957668.1|4460302_4461277_+|hypothetical-protein
MRFSMPENNRCTDFAIAIAWPETYCKQPGYWYDRFTNLLGVSDNHYYKVGHAALVLINSKTKKCHYFDFGRYVAPFQHGRVRSEITDHGLKVNTLAQISEDGQRIENYNEILTELQLNHECHGEGALHASYSMIEFEKAYQKVLHLQRRGPIQYGPFQYKGSNCSRFVNTSILSGKPQWKYAFRLKYFVPLTPTPLNNVNALPNKVVIPKLLKTKAFCPAPVSDKRKLKLTLVEPPRMNNIPENAMWLSGEGSGSWFVINQKFRNYHISRYGPEGDLECKGIFKIAGNSHFDIILPFQIDHLSHCQRVIINQSENLIALIRIAD
>NZ_AP018042.1|WP_162845483.1|4458906_4460322_+|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme
MKDIEILSGAFEKIRAYIQENQNDSQPVVKFKSPEELKEVFDFKVGENGVSENEFLELLDKYLEYSVRTGNKQFLNQLYSGFNFPAFIGEVFSVLANTSMYTYEVAPVATVIETEMISLMNSYAGYSDGDGIFVSGGSNANLIAMFSARNRIFPDSRFEGYDRNNKLVAFVNEQAHYSFETAANILGIGAKNVIKVKADKNGKLISSELEKEIAKAISRGEKPFFAVATCATTLLGAYDPIEEMAEICKKHNIWLHADGSFGGSLILSDKNRYLMKGIEQTDSFAWNPHKLMNIPLICSALLVKKRGTLQHNITDINTDYIFHDIDAIEDLGKKSIQCGRKVDAVKLWFAWKYFGLEGYQKRIDNLIDMAVYAENIVNENPQLELLSERNSFAVCFRYIPKNESDLNKFNLELRESLRKSGKSIVNYGYIGKTLAIRLITANGELQKSDIDLFFANLLFEVEKLENAIFNA
>NZ_AP018042.1|WP_096431457.1|4458188_4458893_+|cysteine-dioxygenase-family-protein
MNTGFSKNIENLINDLRNQNSVTNPMVLEMVESYKLTEQDVAQYFSYNHSPDESYGRQLIYDNGNFKILLMSWKPGDFTAIHNHGYTEWGCVYFFGEATHRLYSVTNDELKIIQKDNFQKGQIASVSGNLTHMMGNSSSKNFSTLHIYGSNSQQSDVSKDAIVYLPELDKEVTTMGSAYLNMDKQLILSEKPLINVSNELVADYFSLVKLFYECNGLSPLLRNMEKKLGNRSIN
>NZ_AP018042.1|WP_096431455.1|4457661_4458177_+|DUF4833-domain-containing-protein
MIKFIFKSVLLLSFGLLSTGLRVAPEDKILFSIGRSKDANEIYYSLNLDKYNRLNAENPIQVYWIKRTRKSKIVPLTWIQQKFAYGIVYLSKSENYAKFRLAAYDKRVFELKKNKNNEFRVFTMSNNVEVEVDRVFIYITGGTFWIPKIPKVELYATLSRTDKKIKETIIP
>NZ_AP018042.1|WP_096431453.1|4456882_4457449_+|YceI-family-protein
MKKVKFLVSMSAIVLTLFAFSPPVGKLVSSKTHVKFFSHTGVEDIQANNYTSISTIAPSTGDVVFSVPMQSFEFEKALMQKHFNSKKFLETKAFPKAKLKGKITNLAQINFEKDGMYLANFEGNLTIKGVTKPIKEKGNITVKGNQIEVQSTFNITLADYGITFVKGKPASNIAKTVEITVHAEYQAK
>NZ_AP018042.1|WP_096431451.1|4456374_4456854_+|hypothetical-protein
MIPKKKKILKIGVILLIAGLLIGGGTVLYMFNMPHRNVQSASTDFSLSASQLVAEYLENPAESNEKYLANDGESKVLEIKGTVAKISEDFKGQKVVLLKGTSDKAGVSCTFTKETNENISNTRVGEIVTIKGVIRLGASFDSDLEMYENVVLEKSDLVK
>NZ_AP018042.1|WP_096431448.1|4455484_4456234_+|thioredoxin-1
MKRILSLVFPCFLFLTACGQNQTIKEVNSTEFEKLIKDSSGTLLDVRTLGEFKNGHIKDAGQLNYYALGFRKKLLLLPKNQAIYLYCNTGYRSQKAAEILAENGYKNIYNLEHGIMEWNLKNLPVLVDLDAKPDTENKMEVDEFTALINSEQLVFADFYAPWCAPCRKMMPMIDSLKTEYKDKITIVKINSDASKKLMKELTVVSVPYLVLYRKGKIIFTHNGSIGKNELVSLFEENRMKYTQVQGDEK
>NZ_AP018042.1|WP_096431469.1|4465720_4466455_+|1-acyl-sn-glycerol-3-phosphate-acyltransferase
MIKILSYILSSIFYILFLLLLVIFHPIQVICHKLFGYQAHKNSVDILNFFLMRVVVFLGVRIASLPKTNLPTDRPLIIISNHQGVYDIPSIVWIFRKHHPKFISKKELSKNIPSVSYNLRHSGAALIDRKNRAQAIPEIYKLGELIARNNYAASIFPEGTRSKTGIVKEFKAGGIEALLKAAPNALLVPFVIDGNYKLEEKGMFPLCLGAKVKYKVLDPIEPANYTAIELTQMVEALIKKELNQ
>NZ_AP018042.1|WP_096431471.1|4466475_4467321_+|patatin-family-protein
MNEINKTALVVEGGAMRGIFAAGVLDQFIEEEFNPFHTVIGVSAGAINASAYLSKQKRRNFKMFTDYSLRPEYISWMKFLRGGHLMDLDWSWDITSRELPIDMDALFASNADFEIGVTMNSDGRSVFIKPDAENISHVMKASCTVPLFYRNFLEIDNQIVSDGGVSAPIPIQRAVEIGASKIMVIRSRPNSFRMKKGMESKLGRLLFRKHPGLVKALQNRPYNYNSSIDFIQNPPQNLQIVDICPPENFKTKRFTQDYNILVDDYELGRKIGKIAIEKWNL
>NZ_AP018042.1|WP_096431473.1|4467507_4469967_+|TonB-dependent-receptor
MNRLILLICLSFVIFAAKAEEPIVNPVMKGGVIKGIVMDNAMDIPVEYATVSVYTMADSSLIDGTITNEKGVFELTKLKPGKYFVEISFIGYNKAIVRNIPINQDRKLADLKIVSLRQSSEALNEVVVTSERVPVQYQIDKKVIPVSRQITAASGTAVDVLENVPSVSVDIEGNVSLRGNSNFTVLVDGKPSILDAADILQQMPASVIENIEIITNPSAKYDPEGTAGIINIITKKNTENGLNGVANLSVGSQDNRSGDILINYRKKKWNWNFGLDYSNRQYEGTSKTENRTMFNDVTSSVLSDGESKMKRSGYGIRTGFDFDVNDKNSISFGFRYGTRDMERGSVLDYQESNSSSPVITSYDSRDLWKKEMTFVNSNFTYVRKFTGKGHQWASQVSYSHRGSGDESSTNELFDSNGQVSGQIATEDGPGTRWEIRSDYTLPLGEDAKFEFGYQGQIRSSEADTEQEDYVVGSGYKPQPLYSHDVTYDRQVHGLYTTYASKIGKFGYQLGFRSEYTSREIDFKDELETFTIKRWDFFPTIHTQMDLGSDNQVMGSYTRRIQRPRGYYLEPFVTWTDAYNVRQGNPDLDPEYIDSYELGYMKRFGDQAISFETYYKVTHNKIERVRYPWEEQGNVMKSTNVNVGNDYSLGIEATFNLSFSTWLKNDLIGNFYHYKEEGDFTITNSANEDTYQDFSTESYNWSLRNNSTFILDPATRLQFTANYNSDTNWAQGKRKGFFTTSAAVKRDFMKRKLSATFQVRDIFGTAKHETLYEGDTFYNFSQFDHNSPTFNLKLSFKINNYKTKRERGSEGGVDVEEFEM
>NZ_AP018042.1|WP_096431475.1|4470146_4470890_-|sulfite-exporter-TauE/SafE-family-protein
MHDITIIQIVLCSIVILSAAFIKGMTGFGFALLAIPFLSFIFPMQVLVPAMSLFNLITSLVILFKLREKIKAYYFIPMFIASLGGIPLGIYALEYVSSDTLRLITGILIILFSIQMLGKVKLAKRFLNFPIVFAGFISGILGSAISVGGPPLVIAMNRKGYSKERFRGVFAWFSTFSSFFTTVAFLMKGMIEMESVNLALFCLPILFFGSHFGSKFSGRIEPEKFRKIVIGINIITGSIIIITAFIN
>NZ_AP018042.1|WP_096431477.1|4470990_4471596_-|MarC-family-protein
MNFNFLEIITSSMVLFAVIDIIGSIPIIVDLQKKGGKIEALKATLVALGVLIAFTFLGQKLLGLFGVDISSFAIAGSFILFIMGVEMALGIEIIKYDGPSGVSIVPIAFPLVAGAGSFTTVLALRAEYAMENLIAAIILNMIFVYIVLRSTDLVERVIGEGGIHILRKVFGIILLAISVKLFMTNVAIQIHTLFPFLKEAL
>NZ_AP018042.1|WP_096431479.1|4471924_4472380_-|methionine-R-sulfoxide-reductase
MKRYIILIGLILFVSNNLLAQKAMTKDKYNVLTPDEKRVIIEKGTEYPGTGEHLNNKTKGVYTCKQCNAELYRSDSKFESNCGWPSFDDEIDGAVKHITDADGRRTEIVCANCNGHLGHAFTGEGFTQKNTRHCVNSISLNFTPTEEIKKK
>NZ_AP018042.1|WP_096431481.1|4472434_4473316_-|alpha/beta-hydrolase
MRRRIRRVLFISGFAILLVIGIVSVLFQLFRAEFDHQKIMSEFHAQNIFPISEMITYEDDQIHTSYVGDLSKPKVLLIHGSPGYWFDFKNIFADKTLQEDYCIISYDRPGYGQTTVPVKKRLHDQAKVAATVLNHYGEPGEKFIVLGHSYGGAVLEQTILDYPAKISHAIYVAPCLSPEFQKAKWYNLMVSGGLANGVLPNELLNSNKEMMALEEDLGNNETRLHEIHIPTSYIQGKEDILVPYQTQAYYLQFHKNVNHILLDDLNHFVPWSAPELIVEAIKNANSSTYYSKF
>NZ_AP018042.1|WP_096431483.1|4473528_4473924_-|response-regulator
MEQMIIDMNNIQNKLILVVEDEEFNRIYFEELLNQIHCNVLVANNGLEAIDICQKNDKIDLVLMDIKMPLMDGYQATKEIKKIRPNLPIIAQTAFALLGDKNKSLENGCDDYIAKPVKKDVLIQLIRKHLA
>NZ_AP018042.1|WP_096431485.1|4474132_4474759_-|chloramphenicol-acetyltransferase
MIEINIDNWERKAQYNFFKDFDDPFMGTTSNIECSQMYMKAKESNESFFIHYMHKALQVINEIPEFRLRILGEKVVQYPIINGTTTVFKHDKTFNFCYFKYIKNFNDFYIQTTNAIELAQKNELLVSKPMQDLIHVSVIPWRSFTSIKHPRIFKDSDSIPKLVFGKVFKTEEKYFMPVSVDAHHALMDGYHVSEFFNKFEVLLSEKQY
>NZ_AP018042.1|WP_153244858.1|4474824_4474983_-|hypothetical-protein
MIRTLCLAYTTSDLSEKSIELNKEILDNHLGSNTQSGERLLTEAVSDEMIKS

Crispr_ID: NZ_AP018042_11

• CRISPR_ID: NZ_AP018042_11
• CRISPR_location: 4660163-4660271
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_11

Consensus_repeat	Method
AGGCTTCCCCGGCATAAAAAGGGAG	CRISPRCasFinder

spacers of NZ_AP018042_11

>11.1|4660188|59|NZ_AP018042|CRISPRCasFinder
TCATTCGCAGGGAGCGAAGGCTATTTTTTTATCATGAAAGCTAATTCGCTTTGGAGCGA

CRISPR arrays and Neighbor proteins around NZ_AP018042_11

CRISPR arrays

The CRISPR arrays of NZ_AP018042_11

>merge|NZ_AP018042|11|4660163-4660271|CRISPRCasFinder
AGGCTTCCCCGGCATAAAAAGGGAGTCATTCGCAGGGAGCGAAGGCTATTTTTTTATCATGAAAGCTAATTCGCTTTGGAGCGAAGGCTTCCCCGGCATAAAAAAGGAG

>NZ_AP018042|11|10|4660163-4660271|CRISPRCasFinder
AGGCTTCCCCGGCATAAAAAGGGAG	TCATTCGCAGGGAGCGAAGGCTATTTTTTTATCATGAAAGCTAATTCGCTTTGGAGCGA
AGGCTTCCCCGGCATAAAAAAGGAG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096431777.1|4651124_4651553_-|acyl-CoA-thioesterase	unknown	unknown	gnl|CDD|223894
NZ_AP018042.1|WP_096431773.1|4647879_4650393_-|DPP-IV-N-terminal-domain-containing-protein	unknown	unknown	gnl|CDD|366374
NZ_AP018042.1|WP_096431805.1|4668281_4669436_-|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	unknown	unknown	gnl|CDD|181749
NZ_AP018042.1|WP_096433749.1|4651646_4652249_-|tetratricopeptide-repeat-protein	unknown	unknown	gnl|CDD|276809
NZ_AP018042.1|WP_096431781.1|4653747_4655970_-|urea-transporter	unknown	unknown	gnl|CDD|377010
NZ_AP018042.1|WP_096431775.1|4650538_4651084_-|DUF1697-domain-containing-protein	unknown	unknown	gnl|CDD|377954
NZ_AP018042.1|WP_096431806.1|4670374_4671775_+|anthranilate-synthase-component-I-family-protein	unknown	unknown	gnl|CDD|223225
NZ_AP018042.1|WP_096431789.1|4658852_4659449_-|AraC-family-transcriptional-regulator	unknown	unknown	gnl|CDD|378964
NZ_AP018042.1|WP_096431779.1|4652290_4653772_-|tetratricopeptide-repeat-protein	unknown	unknown	gnl|CDD|276809
NZ_AP018042.1|WP_096431799.1|4664976_4666011_-|3-dehydroquinate-synthase	unknown	unknown	gnl|CDD|341474
NZ_AP018042.1|WP_162845489.1|4658423_4658666_-|PqqD-family-protein	unknown	unknown	gnl|CDD|377508
NZ_AP018042.1|WP_096431785.1|4657375_4658422_-|ATP-grasp-domain-containing-protein	unknown	unknown	gnl|CDD|237195
NZ_AP018042.1|WP_096433751.1|4662112_4663093_-|chorismate-synthase	unknown	unknown	gnl|CDD|376510
NZ_AP018042.1|WP_096431795.1|4663160_4663625_-|DUF523-domain-containing-protein	unknown	unknown	gnl|CDD|377367
NZ_AP018042.1|WP_096431797.1|4663678_4664956_-|3-phosphoshikimate-1-carboxyvinyltransferase	unknown	unknown	gnl|CDD|235037
NZ_AP018042.1|WP_096431783.1|4655972_4657319_-|alanine-racemase	unknown	unknown	gnl|CDD|143508
NZ_AP018042.1|WP_096431807.1|4671778_4672348_+|aminodeoxychorismate/anthranilate-synthase-component-II	unknown	unknown	gnl|CDD|235552
NZ_AP018042.1|WP_096431803.1|4667362_4668262_-|prephenate-dehydrogenase	unknown	unknown	gnl|CDD|181452
NZ_AP018042.1|WP_096431801.1|4666090_4667176_-|bifunctional-3-deoxy-7-phosphoheptulonate-synthase/chorismate-mutase-type-II	unknown	unknown	gnl|CDD|273577
NZ_AP018042.1|WP_096431793.1|4661026_4661953_-|hypothetical-protein	unknown	unknown	gnl|CDD|367655

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096431777.1|4651124_4651553_-|acyl-CoA-thioesterase	gnl|CDD|223894	COG0824, FcbC, Predicted thioesterase [General function prediction only].	1.85561e-28
NZ_AP018042.1|WP_096431773.1|4647879_4650393_-|DPP-IV-N-terminal-domain-containing-protein	gnl|CDD|366374	pfam00930, DPPIV_N, Dipeptidyl peptidase IV (DPP IV) N-terminal region. This family is an alignment of the region to the N-terminal side of the active site. The Prosite motif does not correspond to this Pfam entry.	4.86487e-39
NZ_AP018042.1|WP_096431805.1|4668281_4669436_-|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	gnl|CDD|181749	PRK09276, PRK09276, LL-diaminopimelate aminotransferase; Provisional.	7.62678e-136
NZ_AP018042.1|WP_096433749.1|4651646_4652249_-|tetratricopeptide-repeat-protein	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	5.74115e-12
NZ_AP018042.1|WP_096431781.1|4653747_4655970_-|urea-transporter	gnl|CDD|377010	pfam03253, UT, Urea transporter. Members of this family transport urea across membranes. The family includes a bacterial homolog.	2.11136e-63
NZ_AP018042.1|WP_096431775.1|4650538_4651084_-|DUF1697-domain-containing-protein	gnl|CDD|377954	pfam08002, DUF1697, Protein of unknown function (DUF1697). This family contains many hypothetical bacterial proteins.	9.27353e-50
NZ_AP018042.1|WP_096431806.1|4670374_4671775_+|anthranilate-synthase-component-I-family-protein	gnl|CDD|223225	COG0147, TrpE, Anthranilate/para-aminobenzoate synthases component I [Amino acid transport and metabolism / Coenzyme metabolism].	4.53466e-134
NZ_AP018042.1|WP_096431789.1|4658852_4659449_-|AraC-family-transcriptional-regulator	gnl|CDD|378964	pfam12833, HTH_18, Helix-turn-helix domain.	2.40405e-12
NZ_AP018042.1|WP_096431779.1|4652290_4653772_-|tetratricopeptide-repeat-protein	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	6.52304e-07
NZ_AP018042.1|WP_096431799.1|4664976_4666011_-|3-dehydroquinate-synthase	gnl|CDD|341474	cd08195, DHQS, Dehydroquinate synthase (DHQS) catalyzes the conversion of DAHP to DHQ in shikimate pathway for aromatic compounds synthesis. Dehydroquinate synthase (DHQS) catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) to dehydroquinate (DHQ) in the second step of the shikimate pathway. This pathway, which involves seven sequential enzymatic steps in the conversion of erythrose 4-phosphate and phosphoenolpyruvate into chorismate for subsequent synthesis of aromatic compounds, is found in bacteria, microbial eukaryotes, and plants, but not in mammals. Therefore, enzymes of this pathway are attractive targets for the development of non-toxic antimicrobial compounds, herbicides and anti-parasitic agents. The activity of DHQS requires nicotinamide adenine dinucleotide (NAD) as cofactor. A single active site in DHQS catalyzes five sequential reactions involving alcohol oxidation, phosphate elimination, carbonyl reduction, ring opening, and intramolecular aldol condensation. The binding of substrates and ligands induces domain conformational changes. In some fungi and protozoa, this domain is fused with the other four domains in shikimate pathway and forms a penta-domain AROM protein, which catalyzes steps 2-6 in the shikimate pathway.	1.03006e-148
NZ_AP018042.1|WP_162845489.1|4658423_4658666_-|PqqD-family-protein	gnl|CDD|377508	pfam05402, PqqD, Coenzyme PQQ synthesis protein D (PqqD). This family contains several bacterial coenzyme PQQ synthesis protein D (PqqD) sequences. This protein is required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis.	3.78322e-06
NZ_AP018042.1|WP_096431785.1|4657375_4658422_-|ATP-grasp-domain-containing-protein	gnl|CDD|237195	PRK12767, PRK12767, carbamoyl phosphate synthase-like protein; Provisional.	9.70212e-32
NZ_AP018042.1|WP_096433751.1|4662112_4663093_-|chorismate-synthase	gnl|CDD|376510	pfam01264, Chorismate_synt, Chorismate synthase.	8.90372e-159
NZ_AP018042.1|WP_096431795.1|4663160_4663625_-|DUF523-domain-containing-protein	gnl|CDD|377367	pfam04463, DUF523, Protein of unknown function (DUF523). Family of uncharacterized bacterial proteins.	1.62824e-57
NZ_AP018042.1|WP_096431797.1|4663678_4664956_-|3-phosphoshikimate-1-carboxyvinyltransferase	gnl|CDD|235037	PRK02427, PRK02427, 3-phosphoshikimate 1-carboxyvinyltransferase; Provisional.	2.59227e-129
NZ_AP018042.1|WP_096431783.1|4655972_4657319_-|alanine-racemase	gnl|CDD|143508	cd06841, PLPDE_III_MccE_like, Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme MccE. This subfamily is composed of uncharacterized proteins with similarity to Escherichia coli MccE, a hypothetical protein that is homologous to eukaryotic ornithine decarboxylase (ODC) and diaminopimelate decarboxylase (DapDC). ODC and DapDC are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases. ODC participates in the formation of putrescine by catalyzing the decarboxylation of ornithine, the first step in polyamine biosynthesis. DapDC participates in the last step of lysine biosynthesis, the conversion of meso-2,6-diaminoheptanedioate to L-lysine. Most members of this subfamily share the same domain architecture as ODC and DapDC. A few members, including Escherichia coli MccE, contain an additional acetyltransferase domain at the C-terminus.	4.39074e-153
NZ_AP018042.1|WP_096431807.1|4671778_4672348_+|aminodeoxychorismate/anthranilate-synthase-component-II	gnl|CDD|235552	PRK05670, PRK05670, anthranilate synthase component II; Provisional.	8.02051e-92
NZ_AP018042.1|WP_096431803.1|4667362_4668262_-|prephenate-dehydrogenase	gnl|CDD|181452	PRK08507, PRK08507, prephenate dehydrogenase; Validated.	1.43734e-127
NZ_AP018042.1|WP_096431801.1|4666090_4667176_-|bifunctional-3-deoxy-7-phosphoheptulonate-synthase/chorismate-mutase-type-II	gnl|CDD|273577	TIGR01361, DAHP_synth_Bsub, 3-deoxy-7-phosphoheptulonate synthase. This model describes one of at least three types of phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase). This enzyme catalyzes the first of 7 steps in the biosynthesis of chorismate, that last common precursor of all three aromatic amino acids and of PABA, ubiquinone and menaquinone. Some members of this family, including an experimentally characterized member from Bacillus subtilis, are bifunctional, with a chorismate mutase domain N-terminal to this region. The member of this family from Synechocystis PCC 6803, CcmA, was shown to be essential for carboxysome formation. However, no other candidate for this enzyme is present in that species, chorismate biosynthesis does occur, other species having this protein lack carboxysomes but appear to make chorismate, and a requirement of CcmA for carboxysome formation does not prohibit a role in chorismate biosynthesis. [Amino acid biosynthesis, Aromatic amino acid family].	2.70963e-58
NZ_AP018042.1|WP_096431793.1|4661026_4661953_-|hypothetical-protein	gnl|CDD|367655	pfam03783, CsgG, Curli production assembly/transport component CsgG. CsgG is an outer membrane-located lipoprotein that is highly resistant to protease digestion. During curli assembly, an adhesive surface fibre, CsgG is required to maintain the stability of CsgA and CsgB.	1.98461e-36

Protein sequences

>NZ_AP018042.1|WP_096431789.1|4658852_4659449_-|AraC-family-transcriptional-regulator
MHQKDNRLHTETIYIKNMVCNCCVRILSMALVKNGIQVDQIRLGKADISYDPNQHNMDTINSILQENGMELVISHEHKIVEQIKTAVIELIHQMNNVDSIVQKSEYLVGKLGLSYQQLSKIFSRHESITLERFIILNKIERIKELIDQDEFTLSEIAFMMDYSSVQYLSSQFHKITGLSVSEYKRNPENHKNPLHSLY
>NZ_AP018042.1|WP_162845489.1|4658423_4658666_-|PqqD-family-protein
MKLKRNIAISENGFLFNPETGDSFIVNPIAKEILNYLEAGYDNEKINQLILDRYEVDLVTLEKNMEDFFTMLQHNELLDR
>NZ_AP018042.1|WP_096431785.1|4657375_4658422_-|ATP-grasp-domain-containing-protein
MEKPKFNIAVTGLNNTDNPGPGIPVIRGLKESKDFDVRIIGLAYENLEPGIYMRDLVDKTYQIPYPSSGKEAVLERIEYINSKENLDFIIPNLDAELYTFIQSKEKLEKMGIRTFLPSMQQFEERHKANLNEYGDKYGIKVPFSKSITNRRDIEELKEDFEYPLLVKGKFYDAYLAYTPEQVNTHFNKISAKWGLPVIIQEFIRGTEVNVIALGDGHGNTVGAVPMRKQYITDKGKAWGGITIQDNKMLDLTKSLIKQTKWRGGMELELIKTNQNEYFLIEINPRIPAWVYLAVAAGQNLPEAMIKLAFGMDVPNYSEYEVGKMFIRYSYDLIGDIAQFGSLSVTGEM
>NZ_AP018042.1|WP_096431783.1|4655972_4657319_-|alanine-racemase
MKKLDYERPILNKVNAGMPDKFGMKTKTATLSHIEEHSVTDILKEYGSPVYVISEPCIRKTYRTAYKAFSTRYPRVQFAWSYKTNYMNAVCKIFHSEGAWAEVVSGFEYSKAIENGISGDQIIFNGPEKSKEDLERAITDGAMIHLDGLDEFYFLGELAEKLNKKANVAIRINLNAGIYPQWDRFGFNLENGEAWHAINRIMLHDNMELVGLHTHMGTYITAVSAYAIAASKLADMAVRLQRKFNHSIKYLDLGGGFATRNTLKGAYLPGSDTCPSFDDYAEALTSALLGSELPPDKLPLLILETGRALIDESGYALGSVLANKRLVDGRRATVIDIGVNMLFTAFWYEHEVMPTKLNSEQTENTTIYGPLCMNIDIIREAIQFPLLQKGDAIVIPRIGAYNMTQWMQFIKLRPPVLLIDEDRKIHLIRKKESFQEFNAQENVPEHLK
>NZ_AP018042.1|WP_096431781.1|4653747_4655970_-|urea-transporter
MKELKQNILFFGEATLNSYAQVFFSRNKAFAVLLLVVSFFDPWAGTAGLISILTANLVAFKMGFSTYYIKDGSYGFNSLLVGLGIGLYFQPGLEITVLVILSSILTLFICLFLQGVFTKYALPFLSVPFLLGIWMIMLASSDLTSLGISERGIYEANEMFSLGGLKLVNVYHFIQNWELHESVSIFLKSLGAIFFQSNWLAGLFILIGLLFYSRIAFTFAILGFYIAYFFYILIGVNITELGYTFIGFNFILTSIALGAFYLIPSPYTYLWIILLLPIVVLTTLSSMHWFAPYRLSVYALPFNVVTLLFLYILKLRHQPSGRLQEVQVQHNSPEKNLYYFKSNVQRLSSNVFPDFQLPFIGEWSVTQAHDGEITHKNEWRHAWDFAIRNDQDEQYKNDGDFVEDYYCYNKTILCPADGEIVNILDGIPDNTIGQVNLEQNWGNSLVIRHDYGFYSQLSHFKEGGFKVKKGDMIKKGEVLGICGNSGRSPYPHLHFQFQATPYIGSATLDVTLPHYIKRQENDLCLQSYSKPKQNDKLLRGEIHPLLSQTFGFQSGQKFTLSVESELDSKLLLLEKNWDFEVKSDAIGTTYIQCLRTNSKAFFSVEANVLQFHNYLGNRRSLLYYLYLSCYKVYLGYYQDLNVKAEIPPNQVFNSSKLFLQDFIAPFYIYLKSDYQLKYQNKKEGISSEKIDLRSKIECGYSNKKEHLSCEIKIESSASLQIKINRNGKLIEIKCGNMQAY
>NZ_AP018042.1|WP_096431779.1|4652290_4653772_-|tetratricopeptide-repeat-protein
MWKHASILILAILLGLSANCQKTLSVAQVDKESFALFQKGQWEQLILEGEKANKQGIDFYYLQYRMAIAYYELKKYRKAASLFHKLYLLNGEDAIIKEYLYFSYLLGGQEKDAIQLQNKLAQSTSEKYGIGKTKVISSIYSEVKNELLDDYAITSDLLQEQTVRKDFLHYSIGLNHQLGERVSLFHAFSHVDVKNSIVSETYLFDEDITQNQYYLKSSIQVGTGTELVIAGHIMQTKIDGNPQTDILTTALSQQTTFTKAGGNGNGPGGGGDGGNGPGGGTDGSGDGNSDGTGTGNETGNGNDPTNTDTADTEIILSPYHFSQNNFIAYLGLTQDISNWKLGAGVLYYSGDDLTRWQQELKLTFYPLGNTNINLSVGFNNQIEETTDGNSLNRSYWKTAIAFRIAKNTWASAGYTFGDLYKQVENQGYSVFNGVNLVKNRKEFSIYQYLFKGKLNVFLIYQNQNEKNNYTINDVINSQNINVQSITGGIKWNF
>NZ_AP018042.1|WP_096433749.1|4651646_4652249_-|tetratricopeptide-repeat-protein
MLIGNAIAQDFQTTGTAFEESYKYEREGDFSNAINQLKAVYDSDSYEINLRLGWLNYQAGVFNESISHYQRAANLKPYAEEAKFGLVLPKAAIGKWDEVVNIYKEILKVSPNQTVANYRLGLIFYGREDFEKAHQHFKKVVDLYPFGYDALIMLAWTNFRMGKSREALILFQKSLWFMPNDPSALEGLKLLGQAQESPSE
>NZ_AP018042.1|WP_096431777.1|4651124_4651553_-|acyl-CoA-thioesterase
MTTDLMMIDMEIGIKAYDIDAMGIVSNIVYIRWFEDLRHLFLEKYFPYEEMIATNISPILIKTEVHYKVPLTIHDHPNGTCRIKNLGSSKWEMDFEISSGDIIHCKGKQVGCFYNLEKKRPSLVPERLLEAYEEAKLQMHDE
>NZ_AP018042.1|WP_096431775.1|4650538_4651084_-|DUF1697-domain-containing-protein
MSKRVKYISVLRGINVGGKRKILMADLKKMYAELGFENCISYIQSGNLIFESKKLSNTELGGKIEDAISEKFGFAVPVIVRTCEEWEQSVLANPYTNSHEKESLCLTFLNETPKAELTAVISELNFEPDSFRIIDKDVFIYCSRKYHQTKLSNQFFESKLKVKATTRNWNTVLKLLELSAE
>NZ_AP018042.1|WP_096431773.1|4647879_4650393_-|DPP-IV-N-terminal-domain-containing-protein
MKRNLLTLLFLLIGIGLFAQEVPVTKANYELAAKFSPNRLKNMVYSTSLTPHWLKNGEKFWYNYKTSEGNIYYLVNPVSNTKVKLFDAVKMAADMSRLTADPFDAQHMYISKLKFINNEKTIQFEVKSKLVEEEEKDDEEDGDKQVEKKDGEEKKKDKKKMVAKVWHFEYQIASEKLNLLDDFEKSLEQKDWASVAPNQEYVIFAKHHNLYWMDAENYKKAQKSEKDSTIIEHELTTDGVEYYSYGSGTYGKTNKEIEEEKDKRKETYVTFSADSKKFALTREDEREVKDLWVINSVASPRPTLEKYKYHMPGEKEAPQYEILIFDWDSKESVKVKAAAFKDQSLSILRAPKKKMNADDDLKFSKWISTNSDVLYFKRQSRDMKRVDICTANTSTGEVKVLIEERLNTYIETKPIGFVNNGKELIQWSERDGWAHFYLYDGEGNLKNQITSGAWHCEKIQGIDEKNRVLYFTGNARENGEDPYYSHLYSVNFDGSGLKLLNKGDFSHDVSLNDQNHYFVNNSSRVNTAPVSALYNAKGNKLMDLETTDLSRLFEAGYQFPEPFKVKAGDGVTDIYGVMYKPFDFDPNKKYPILTYVYPGPQTEAVYKSFSTRMDRADRMAQLGFIVVTIGNRGGHPARSKWYHNYGYGNLRDYGLEDKKVGLEQLANRFDFIDINKVGIFGHSGGGFMSTAAMLVYPDFFKVAVSAAGNHDNNIYNRWWSETHHGVKEEISEKGDTTFTYKIDTNPSLAKNLKGKLMIVTGDIDNNVHPGNTIRMANALIKANKRFDFFIYPGQRHGFGDMSEYSFWLRGEYFCKYLLGDFRNSADYVEMQNDQPRN
>NZ_AP018042.1|WP_096431793.1|4661026_4661953_-|hypothetical-protein
MRNYGNLLRICGYFVLFCFLATKISAQEPQIKKRIAVFVFEDKTDKSWRWWNNKGVGDGVSDMLTTALVKSGNYRVIERAEIDRILNEQDFGQSGRVTQQSAAQMGSVLGVEIAVIGSVSEFGYKKGETGGAIRGLGVGVSNQSATVGVDVRMVNTTTGEIVTAENVRKQKSAKGLKLRTNKLNFKDRKDFDESLVGKAAREAIEDIVSMIDNNANNIEWQAKVITEKAGVVFINSGDADGLKIGDVLSVYSKGEDLIDPDTGISLGSVDSKIGEIKITDSSIGNGKASKCSIVKGTGFAKGNFVRLK
>NZ_AP018042.1|WP_096433751.1|4662112_4663093_-|chorismate-synthase
MNNFGRIFRLSIFGESHGKGVGITIDGCPSGIDLCDEDLLADISRRKAGAKGTTPRIEADMPSILSGTFEGKTTGAPLIILFHNKNTKSKDYSNLLDSPRPGHADFVAQHKYGRHNDYTGGGHFSGRITLGIVAAGVVAKKILGDVKIDAKLTEVGGQSDFDAAVEDALLQHDSIGGIVECRANNLPIGYGEPFFDSVESMISHLIFAIPATKGIEFGSGFAAAKMKGSEHNDNFIDATGKTETNHAGGINGGITNGNELVFRVAIKPTSSIGINQKTMNFAKGEVEDLLIEGRHDACIALRVPVIIEAATAIALADLKLLDKARR
>NZ_AP018042.1|WP_096431795.1|4663160_4663625_-|DUF523-domain-containing-protein
MIIVSACLAGCECRYDGKSNEVPEIKKLVLENKAIALCPEELGGLSTPRIPCEIVKVNNEVKVIGRDKNDYTAQFTKGAEIFTNVAKALNCKTVILKANSPSCGYGKIYDGSFTGKYRKGNGFTAEKLFDLNISILNETNFNDFFKEESELQSD
>NZ_AP018042.1|WP_096431797.1|4663678_4664956_-|3-phosphoshikimate-1-carboxyvinyltransferase
MRVRIQSSTIKGRLTPPSSKSMMQRALAAAMLANGDSVIHNPCKCEDADAATDIIQRMGSVVTKKENSLEITSKVSEAPKSIHVGESGLSLRMFSPIVALKPEEMEITGTGSILNRPVQNIIEGLENLGIECENPGKGFLPIRIKGGYKSNFAKIDGSLGSQFLTGLLMALPLRKEDTTLKVSNLKSIPYINMTLDLIKHFGIEIEHENYENFQIKGNQIYQPTEYTIEADWSSAAPLLVAGALKGIVTLEHMNPDSYQADKAILDALQKCGASIAWEGNNLTVEKKDLNAFDFDATHSPDLFPPLVALAAHCVGESRITGVHRLEHKECNRGVVLKKEFAKLGVRIRIEGDEMIIPGGDVQGGLAKSNNDHRIAMALAVTALASPSPIEIGQHECVAKSYPEFFTDLRKIGGGVAQFYFDSQFV
>NZ_AP018042.1|WP_096431799.1|4664976_4666011_-|3-dehydroquinate-synthase
MKTIHIEKQNSSIFVGESYLNVNKYLPEKKCFLICDENIYQHYSEFINQFDYIVMGCGEGNKNWQTIEKIIDQLLERGADRNSFLLGMGGGLVSDVTGFVASIFMRGLEFGFISTSLLSQVDASVGGKNGINFGKLKNMIGIFNPPKFVICDPVLLQTLPKREVRSGFGEVIKHAFIKDEDLYNFLNENSEALLNLDPNLMEDLIYRAVSIKTEVVEGDPFEKGERKKLNFGHTIGHAIENNSDLTHGEAVSVGMSLACRISQELCELSDANVKQVNDLLRKFELPLEHDVSADTINQYLIKDKKKNRSTIDFILLKKIGEATIVTLPIEELKTKVIGLCANPK
>NZ_AP018042.1|WP_096431801.1|4666090_4667176_-|bifunctional-3-deoxy-7-phosphoheptulonate-synthase/chorismate-mutase-type-II
MKNLEIKDLSSWPIDVSTRPLVIAGPCSAETEEQVMEAARGVKAAGIQIFRAGIWKPRTRPNSFEGIGAKGLIWLQKVKEEFGLITATEVANARHVELALGAGVDILWIGARTTVNPFAVQEIADALRGHDIPVLVKNPINPDLELWMGAIERLHAVGLTRLAAIHRGFSTYQKLKYRNDPQWQIPIELKRRMPQLPIFCDPSHITGDRDLLDEVAQKSMDLNYEGLIIESHCKPEEAWSDAKQQLTPIALKELLANLVLRDPEAPNELVDSTLGELRHLIDDFDQKLLELLENRMQVSKTIGHYKKENNITVLQNKRWGSILEKSMENGKKKGFSEQFINSLFKAIHQESINQQEEIMNS
>NZ_AP018042.1|WP_096431803.1|4667362_4668262_-|prephenate-dehydrogenase
MILSVIGLGLIGGSIALDLKKRGFVSKVIGVENNTLNAHAAKKLGLVDEIQDLKTAVCSSDLVILAIPVDACLRLLPDIMNLIDHQIVTDLGSTKEQLIESIANHPKRNQFVPAHPMAGTEFSGPQAAHSGLFDGKCSIICNPEDSDKKAVNLITEMFHCLSMRCVFMDAEVHDRQTAYVSHLSHISAFALSLTVLHKEKSEKHIFELASGGFDSTVRLAKSSAEMWTPIFQQNKKNIGKVLQKYINTLQQFKDLIEEDNLTGVNDLIFEANDIRRILDQKEKNEKETIENTEKIKHKA
>NZ_AP018042.1|WP_096431805.1|4668281_4669436_-|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme
MIQPANRTQGVKEYYFSVKLKEIAKLNEGAVKVLNLGIGDPDMSPSEDTWEMLKEACAHPKSHGYQSYVGIPALREGFSKWYKTFFNVDLNPDNEILPLLGSKEGVMITSLAYLNEGDEVLIPNPSYPTYTSVTNLLGGKIVSYPLVEENNYHPDFDALEKRDLSKVKIMWVNYPHMPTGARASKELLEKIVAFGLKHKILIVNDNPYSFILNDTPLSIMSIEGAKDCCIELNSLSKSHNMPGWRVGMICGKAEMLEPVLKVKTNMDSGMFYPVQQAAIKALAEDQSWYDSINKVYKIRRDLVFKLMNLIGCSPREDQGGMFVWAAIPKKYKDGYELSDKILKESKVFITPGGIFGDQGDKYIRISLCSSEAGIEEAIERIKEN
>NZ_AP018042.1|WP_096431806.1|4670374_4671775_+|anthranilate-synthase-component-I-family-protein
MIKFKYLKKELLADVITPVSMYLKLRDRFPGAILLESSDYHSPENASSFIALEPIASITLKDGRLIEEIEGKKEVCDARGKGKRFVSGKLKDFVGQFDLEGKENVPKANALFGYTTFDAVPYFEDLDFNLEKKQSGIPQMCYSLYRFIIEVNHFNNTLKIIELVKNGDDSRIREVSDLLRNRNLPAFTFSLEGEEQSNMDDETYLNMVTQGKKHCKRGDVFQIVLSRQFSHAYKGDDFNLYRALRSVNPSPYLFYFDYGSFRIMGSSPEAQIEIKNKKAYIHPIAGTFRRTGDAVKDAELAKELLKDEKEQAEHVMLVDLARNDLSRDAQNIKVETFCEVQYFSHVIHLVSKVSGELPDDYNPFQLMGDTFPAGTLSGAPKYRAMQLIDEYEPTARGFYGGCIGSLGFNGEVNQAIMIRSFMSKDNHLYFQAGAGVVDKSIEENELKEVSNKLAALRKAMELAQTI
>NZ_AP018042.1|WP_096431807.1|4671778_4672348_+|aminodeoxychorismate/anthranilate-synthase-component-II
MKILVLDNYDSFTYNLVEYLRNLGANDIEVHRNREIDLKEIAKFDKILLSPGPGIPDEAGILKDVIREYAATKSILGVCLGEQAIAEVFGGSIENLDKVYHGVATSVFRTDDDKGVLQGIDQEFTAGRYHSWNVTKENLPDCLIVTCEDAEGQIMGIRHKEYDVQGVQFHPESVLTPNGLKMIENWLNN

Crispr_ID: NZ_AP018042_12

• CRISPR_ID: NZ_AP018042_12
• CRISPR_location: 5049715-5049802
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP018042_12

Consensus_repeat	Method
CTTTCTCTGACAAAGAGTCACTTTTG	CRISPRCasFinder

spacers of NZ_AP018042_12

>12.1|5049741|36|NZ_AP018042|CRISPRCasFinder
ACCATCGGGAATTCATTTTGGGGGTTCGAGAATCTG

CRISPR arrays and Neighbor proteins around NZ_AP018042_12

CRISPR arrays

The CRISPR arrays of NZ_AP018042_12

>merge|NZ_AP018042|12|5049715-5049802|CRISPRCasFinder
CTTTCTCTGACAAAGAGTCACTTTTGACCATCGGGAATTCATTTTGGGGGTTCGAGAATCTGCTTTTTCTGACAAAGAGTCACTTTTG

>NZ_AP018042|12|11|5049715-5049802|CRISPRCasFinder
CTTTCTCTGACAAAGAGTCACTTTTG	ACCATCGGGAATTCATTTTGGGGGTTCGAGAATCTG
CTTTTTCTGACAAAGAGTCACTTTTG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096432373.1|5052964_5053669_-|uroporphyrinogen-III-synthase	unknown	unknown	gnl|CDD|119440
NZ_AP018042.1|WP_096432347.1|5038087_5038393_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096432353.1|5041118_5042045_+|hydroxymethylbilane-synthase	unknown	unknown	gnl|CDD|234612
NZ_AP018042.1|WP_096432377.1|5056394_5058173_+|DNA-polymerase-III-subunit-gamma/tau	unknown	unknown	gnl|CDD|237874
NZ_AP018042.1|WP_096432355.1|5042129_5043122_+|porphobilinogen-synthase	unknown	unknown	gnl|CDD|236450
NZ_AP018042.1|WP_096432380.1|5060753_5062352_-|cation:proton-antiporter	unknown	unknown	gnl|CDD|226998
NZ_AP018042.1|WP_096432365.1|5047775_5048174_-|permease	unknown	unknown	unknown
NZ_AP018042.1|WP_096432375.1|5053978_5056180_+|bifunctional-(p)ppGpp-synthetase/guanosine-3',5'-bis(diphosphate)-3'-pyrophosphohydrolase	unknown	unknown	gnl|CDD|223394
NZ_AP018042.1|WP_096432369.1|5050634_5051573_-|pyridoxal-phosphate-dependent-enzyme	unknown	unknown	gnl|CDD|107205
NZ_AP018042.1|WP_096432359.1|5044340_5045429_+|SUMF1/EgtB/PvdO-family-nonheme-iron-enzyme	unknown	unknown	gnl|CDD|335469
NZ_AP018042.1|WP_096432357.1|5043182_5044214_+|uroporphyrinogen-decarboxylase	unknown	unknown	gnl|CDD|234644
NZ_AP018042.1|WP_096432371.1|5051659_5052952_-|glutamate-1-semialdehyde-2,1-aminomutase	unknown	unknown	gnl|CDD|234607
NZ_AP018042.1|WP_096432363.1|5046974_5047721_+|3-hydroxyacyl-ACP-dehydratase	unknown	unknown	gnl|CDD|224835
NZ_AP018042.1|WP_145957691.1|5063222_5063606_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096432382.1|5062606_5063119_+|ferritin	unknown	unknown	gnl|CDD|153113
NZ_AP018042.1|WP_096432349.1|5038635_5039472_-|NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl|CDD|187576
NZ_AP018042.1|WP_096432367.1|5050032_5050542_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096432361.1|5045615_5046890_+|2-hydroxyacyl-CoA-dehydratase	unknown	unknown	gnl|CDD|377594
NZ_AP018042.1|WP_096432351.1|5039756_5041010_+|glutamyl-tRNA-reductase	unknown	unknown	gnl|CDD|234592
NZ_AP018042.1|WP_096432378.1|5058419_5060645_+|NADP-dependent-isocitrate-dehydrogenase	unknown	unknown	gnl|CDD|377178

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096432375.1|5053978_5056180_+|bifunctional-(p)ppGpp-synthetase/guanosine-3',5'-bis(diphosphate)-3'-pyrophosphohydrolase	gnl|CDD|223394	COG0317, SpoT, Guanosine polyphosphate pyrophosphohydrolases/synthetases [Signal transduction mechanisms / Transcription].	0
NZ_AP018042.1|WP_096432378.1|5058419_5060645_+|NADP-dependent-isocitrate-dehydrogenase	gnl|CDD|377178	pfam03971, IDH, Monomeric isocitrate dehydrogenase. NADP(+)-dependent isocitrate dehydrogenase (ICD) is an important enzyme of the intermediary metabolism, as it controls the carbon flux within the citric acid cycle and supplies the cell with 2-oxoglutarate EC:1.1.1.42 and NADPH for biosynthetic purposes.	0
NZ_AP018042.1|WP_096432353.1|5041118_5042045_+|hydroxymethylbilane-synthase	gnl|CDD|234612	PRK00072, hemC, porphobilinogen deaminase; Reviewed.	4.87999e-137
NZ_AP018042.1|WP_096432369.1|5050634_5051573_-|pyridoxal-phosphate-dependent-enzyme	gnl|CDD|107205	cd01562, Thr-dehyd, Threonine dehydratase: The first step in amino acid degradation is the removal of nitrogen. Although the nitrogen atoms of most amino acids are transferred to alpha-ketoglutarate before removal, the alpha-amino group of threonine can be directly converted into NH4+. The direct deamination is catalyzed by threonine dehydratase, in which pyridoxal phosphate (PLP) is the prosthetic group. Threonine dehydratase is widely distributed in all three major phylogenetic divisions.	1.86268e-141
NZ_AP018042.1|WP_096432380.1|5060753_5062352_-|cation:proton-antiporter	gnl|CDD|226998	COG4651, RosB, Kef-type K+ transport system, predicted NAD-binding component [Inorganic ion transport and metabolism].	4.79825e-69
NZ_AP018042.1|WP_096432373.1|5052964_5053669_-|uroporphyrinogen-III-synthase	gnl|CDD|119440	cd06578, HemD, Uroporphyrinogen-III synthase (HemD) catalyzes the asymmetrical cyclization of tetrapyrrole (linear) to uroporphyrinogen-III, the fourth step in the biosynthesis of heme. This ubiquitous enzyme is present in eukaryotes, bacteria and archaea. Mutations in the human uroporphyrinogen-III synthase gene cause congenital erythropoietic porphyria, a recessive inborn error of metabolism also known as Gunther disease.	3.40593e-32
NZ_AP018042.1|WP_096432361.1|5045615_5046890_+|2-hydroxyacyl-CoA-dehydratase	gnl|CDD|377594	pfam06050, HGD-D, 2-hydroxyglutaryl-CoA dehydratase, D-component. Degradation of glutamate via the hydroxyglutarate pathway involves the syn-elimination of water from 2-hydroxyglutaryl-CoA. This anaerobic process is catalyzed by 2-hydroxyglutaryl-CoA dehydratase, an enzyme with two components (A and D) that reversibly associate during reaction cycles. This component contains one non-reducible [4Fe-4S]2+ cluster and a reduced riboflavin 5'-monophosphate.	6.38216e-133
NZ_AP018042.1|WP_096432377.1|5056394_5058173_+|DNA-polymerase-III-subunit-gamma/tau	gnl|CDD|237874	PRK14971, PRK14971, DNA polymerase III subunit gamma/tau.	0
NZ_AP018042.1|WP_096432359.1|5044340_5045429_+|SUMF1/EgtB/PvdO-family-nonheme-iron-enzyme	gnl|CDD|335469	pfam03781, FGE-sulfatase, Sulfatase-modifying factor enzyme 1. This domain is found in eukaryotic proteins required for post-translational sulfatase modification (SUMF1). These proteins are associated with the rare disorder multiple sulfatase deficiency (MSD). The protein product of the SUMF1 gene is FGE, formylglycine (FGly),-generating enzyme, which is a sulfatase. Sulfatases are enzymes essential for degradation and remodelling of sulfate esters, and formylglycine (FGly), the key catalytic in the active site, is unique to sulfatases. FGE is localized to the endoplasmic reticulum (ER) and interacts with and modifies the unfolded form of newly synthesized sulfatases. FGE is a single-domain monomer with a surprising paucity of secondary structure that adopts a unique fold which is stabilized by two Ca2+ ions. The effect of all mutations found in MSD patients is explained by the FGE structure, providing a molecular basis for MSD. A redox-active disulfide bond is present in the active site of FGE. An oxidized cysteine residue, possibly cysteine sulfenic acid, has been detected that may allow formulation of a structure-based mechanism for FGly formation from cysteine residues in all sulfatases. In Mycobacteria and Treponema denticola this enzyme functions as an iron(II)-dependent oxidoreductase.	4.57023e-86
NZ_AP018042.1|WP_096432357.1|5043182_5044214_+|uroporphyrinogen-decarboxylase	gnl|CDD|234644	PRK00115, hemE, uroporphyrinogen decarboxylase; Validated.	2.34973e-118
NZ_AP018042.1|WP_096432371.1|5051659_5052952_-|glutamate-1-semialdehyde-2,1-aminomutase	gnl|CDD|234607	PRK00062, PRK00062, glutamate-1-semialdehyde 2,1-aminomutase.	0
NZ_AP018042.1|WP_096432363.1|5046974_5047721_+|3-hydroxyacyl-ACP-dehydratase	gnl|CDD|224835	COG1924, COG1924, Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) [Lipid metabolism].	5.72524e-68
NZ_AP018042.1|WP_096432382.1|5062606_5063119_+|ferritin	gnl|CDD|153113	cd01055, Nonheme_Ferritin, nonheme-containing ferritins. Nonheme Ferritin domain, found in archaea and bacteria, is a member of a broad superfamily of ferritin-like diiron-carboxylate proteins. The ferritin protein shell is composed of 24 protein subunits arranged in 432 symmetry. Each protein subunit, a four-helix bundle with a fifth short terminal helix, contains a dinuclear ferroxidase center (H type). Unique to this group of proteins is a third metal site in the ferroxidase center. Iron storage involves the uptake of iron (II) at the protein shell, its oxidation by molecular oxygen at the ferroxidase centers, and the movement of iron (III) into the cavity for deposition as ferrihydrite.	6.72523e-79
NZ_AP018042.1|WP_096432349.1|5038635_5039472_-|NAD(P)-dependent-oxidoreductase	gnl|CDD|187576	cd05266, SDR_a4, atypical (a) SDRs, subgroup 4. Atypical SDRs in this subgroup are poorly defined, one member is identified as a putative NAD-dependent epimerase/dehydratase. Atypical SDRs are distinct from classical SDRs. Members of this subgroup have a glycine-rich NAD(P)-binding motif that is related to, but is different from, the archetypical SDRs, GXGXXG. This subgroup also lacks most of the characteristic active site residues of the SDRs; however, the upstream Ser is present at the usual place, and some potential catalytic residues are present in place of the usual YXXXK active site motif. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Atypical SDRs include biliverdin IX beta reductase (BVR-B,aka flavin reductase), NMRa (a negative transcriptional regulator of various fungi), progesterone 5-beta-reductase like proteins, phenylcoumaran benzylic ether and pinoresinol-lariciresinol reductases, phenylpropene synthases, eugenol synthase, triphenylmethane reductase, isoflavone reductases, and others. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. In addition to the Rossmann fold core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	1.87489e-60
NZ_AP018042.1|WP_096432355.1|5042129_5043122_+|porphobilinogen-synthase	gnl|CDD|236450	PRK09283, PRK09283, porphobilinogen synthase.	0
NZ_AP018042.1|WP_096432351.1|5039756_5041010_+|glutamyl-tRNA-reductase	gnl|CDD|234592	PRK00045, hemA, glutamyl-tRNA reductase; Reviewed.	3.00384e-112

Protein sequences

>NZ_AP018042.1|WP_096432365.1|5047775_5048174_-|permease
MRKEEKLYEVFGELLYALAKADGVIKKEEKEILKQLLKNHALGSEILWSFEFEESHNSSVEEIYNKVINYCHVYGPAPQYDEFINAMKIVADASDGITSKEHNLINSFSEDLMERFQRDIDKLKQFEKKEYW
>NZ_AP018042.1|WP_096432363.1|5046974_5047721_+|3-hydroxyacyl-ACP-dehydratase
MRHAGIDIGSRSIELVVIEEGKVIVRKQAESGYNPVERVEELIADISFDKVMATGYGRASLEIAFDYSTVTEIKAYGIGAAALHPGIRGILDIGGQDTKVISLNENGKVFKFEMNDKCAAGTGKFLEMMAVTLGYKQETFGLEALKGQEGIEINSMCAVFAESEVTSLLAKGCQRADIAMAIHQSVCRRAVGMIKRQDLKAPMLFAGGVANNSCMIHLLQQTFGDDIVIPESPQFVGAYGAAILAGSL
>NZ_AP018042.1|WP_096432361.1|5045615_5046890_+|2-hydroxyacyl-CoA-dehydratase
MADYTKMWTELGLDLDNHDALLENLGGAYQSIFMSQEKRPEGMGYFDFVMSEAHGLRIQELRDEQEAGRKIIGSFCVFVPEELVLAAGCTSVGLCAGADFAQEEVEKVLPRNTCSLIKSFFGFKLGKVCPYMEASDLIVGENTCDGKKKSFETFSQLVPNFYQMDLPQNKAPMARKLLKHEYMAFVAKLEEMTGKSISLEDLKQGIATVNAKRNALARLSKLRSADPSPISGLDALLINQIAFLDNPARFTEKVNVLCDELELFVKENKGVKAVKAPRVVISGCPMAIPNWKVPAIIESTGAVIVGEESCIGERGQRNTTDDSSDTLDGLIDAVVDRYFKIDCAVFTPNQERVEHIKEMAKNTNADGVIHYGLQFCQPYIMESFSVEKILEKDGVSVLRLETDYSQEDMGQLSTRVEAFVEIIK
>NZ_AP018042.1|WP_096432359.1|5044340_5045429_+|SUMF1/EgtB/PvdO-family-nonheme-iron-enzyme
MSKIQIFCIITSMLFVACKSTPKETSITIENKAEKKACCENIPNRFTSEKEVKADEMVWIEGGTFMMGGDDKQARDDEFPKHEVTVSGFYMDAHEVTNAQFMEFAEATGYVTEAEKKPDWEEIKKQLPPGTPEPDDSVLAPASLTFFAPNHSVNLNNNAAWWAWTLGANWRHPQGPDSSIDNKLNFPVVHVSWNDVMAYCKWAGKRLPTEAEWEFAARGGLIDNIYPWGNEDIDLGKIKANSWQGQFPNINEEKDGFYKSAPVKTFAPNEYGLYDMAGNVWEWCSDTYHYDYYKTIKGTSVNPKGPETSYDPNEPGVFKKVTRGGSFLCNTAYCSGYRVAARMKSSIDTGMEHTGFRCVKDK
>NZ_AP018042.1|WP_096432357.1|5043182_5044214_+|uroporphyrinogen-decarboxylase
MSQSIFLDTINGIKRERPPVWFMRQAGRVLPSYMKLREKYGFKELMENPELAAQVTLLPIHDLGVDAAILFSDILVIPEALGMELSFEGKGPSFSTALKDVADPLSFLSDKPEKLEHIYKAIDRILETKPNEIPLIGFCGGPLTTLCYMYQGFSRNQNFPDFVPAIYRDKALMKKVVAKITEMSIHYALKQVEHGVKAFQLFETHAGLIPVELYKEVFLPSVRAILSAVRETGVKTIYLPKGLGTGINMVNYDLCDCVSVDWQMPLHEVRGIVGEEMILQGNFDPRILEATPQAIDQEFEYFLNYGRKDHKWIFNLGHGLLPSIPVENVEYLVKKVKESDWGR
>NZ_AP018042.1|WP_096432355.1|5042129_5043122_+|porphobilinogen-synthase
MSLRPLFPDTRLRRLRYSKVVRNMVAETSLSVDDLIMPLFVCPGENVQNPIKSMPGNDQLSVDMLVKKCGELLESGVKSVLLFGIPESKDEDGTVATHEHSIVQKATRAIKETYPEMFIIADICNCEYTTHGHCGTIVDGDVDNDQTLVTLAAQAVSLAEAGVDMVAPSDMMDGRIGYMRKALDENCFEKMPIMAYSAKYASGFYGPFRDAAGSAPQFGDRSTYQMDPRNSDEAIREVEMDIAEGADIVMVKPAMSYLDVIYRVKNEFNIPVAAYNVSGEFSMVKAAEANDWIDGKRVMMEIMTSIKRAGADIIITYHAKEVADILNGKM
>NZ_AP018042.1|WP_096432353.1|5041118_5042045_+|hydroxymethylbilane-synthase
MISKKKIVVATRPSLLAYTQTMQTVELLRKANPEIEFEVKKFSTQGDRVLNRALTEFGGTGVFVKELEHALLENEADIAIHSLKDVPSAHPAGLQLVAFPEREDSRDVFLTRDGSSIDDMPEGFVLGTGSPRRRVQFANLRKDIVFKEIRGNIDSRFQKLMDGEYDAIILAAAGLNRMSKQYDENMLLDVDLCIPAIGQGALAIECRSNDAETIAILEKVNHRDTEKAVLAERAFMAEIEGGCKFPLAAHAVVSDNTLNMIAIVGDLKTNQFIVEGIEVSVIESVEKSKELAIKMKAICKEKGINFYL
>NZ_AP018042.1|WP_096432351.1|5039756_5041010_+|glutamyl-tRNA-reductase
MSSQIRENLQKLFVLGISYKKTALEVRGKFSLTAEQREDLLKEAKENQIDGIVVLSTCNRTEIYAHSSESEFITNLFLKYSEGDQEDFASNGYTLFGNDCIHHLYRVTSGLDSQIIGDFQIVGQVKNAVQLSEEMSLMNPFLNRLFSFAFQASKKIKTNTEISKGAASVSHAAVQYIKDKVSTLESCNFLLYGTGDIGKDTCGNLLKHMSNRSLTLINRTVSKAEALANKFNIQYKPVANLKEEVDKAEVIIVATGAPQPTLLPEHFNGSEKCKLILDLSVPRNVHPEIDKLAHVLVITVDELSEHISEAIQQRKDCIPQAEGIIYGSIAEFYSWLEIKYLSPVIIALKENLQRVQQKELDYHKNKLTDDELKKVEHITSNIVNKIARACINHLKDHHKKQSSPMETIDMIFKDMDS
>NZ_AP018042.1|WP_096432349.1|5038635_5039472_-|NAD(P)-dependent-oxidoreductase
MNKTLYKPEKTISILGCGWLGLPLAKFFIKNGYSVKGSTPTPEKISILAENGIIPYRILLNPEINTDFDADFFNSEIIVVNFPPKRREDIEEFHPQQFKALIEQVRLSSIKKLIFISSTSVYANLNRIVTEADNQISEKNSGKALRVVENMLLAQDDFKTSIIRFGGLIGYDRKPGRFFSKMKNAVEGDTPVNLIHQDDCIGIISHVIENGLWGETYNACCPEHPTRKEFYIKAAKSGGFELPQFVANKSTFKIISSAKLEKTNYKFKYANPIEALAF
>NZ_AP018042.1|WP_096432347.1|5038087_5038393_-|hypothetical-protein
MIYLLIKQSIKNFVRWKSSFDQFHEYRKVGGELSCEIYATNEKRDEHIILSQWKSEASALEYLESQSFEMIKELEGEEPVSIQFLPIAELANFKSNHQLGS
>NZ_AP018042.1|WP_096432367.1|5050032_5050542_-|hypothetical-protein
MELKKLLDPFPVKEEAKLVAQSIAENPMYMKELWEICISNEKHSWRATWILDKVYDIDPDLVRLYLPQMIKLVPTLSNESKLRQYLKLISLEPLPSNISGDFINYCFDALISSTSAIAIKVYTMQILYNFSLQEPDIQNELALVIEEQMENGSAGLYSRGRRILKAINK
>NZ_AP018042.1|WP_096432369.1|5050634_5051573_-|pyridoxal-phosphate-dependent-enzyme
MITKESLQETQQRIHKFIHRTPIMSSRLINDIVGADIYFKCENFQKMGAFKMRGATNAILQLSETEKEFGVATHSSGNFAQAIALAAKVQGIKAFIVMPSNAPEIKKTAVKGYGGEIIECIPTLEARETTLNEVVNKTGAVFLHPYNDYNVIQGQATAAMELIEEHDDLDAIFAPVGGGGLLSGTALAAHYFSPSTKIVAGEPMGADDAWQSMQKGEIVPQTNPKTIADGLLTSLGDKTFPIIKEHVEKIIRVEENEIIAAMRLIWERMKLIVEPSSAVALAALIKEKKKYKGQKIGVILSGGNVELSKLPF
>NZ_AP018042.1|WP_096432371.1|5051659_5052952_-|glutamate-1-semialdehyde-2,1-aminomutase
MYQYKRSSELFTEANKYIPGGVNSPVRAFNSVGGTPVFIKKAKGSILTDVDGNEYIDYISSWGPMILGHAYPKVIDAIKAKVEDSTSFGAPTELEIDIAKLIVDMVPNIDKVRMVNSGTEACMSAIRVARGYTGRNKFIKFEGCYHGHADSFLIKAGSGASTFGVPNSPGVTPGTANDTLTAKYNNIEEVKQLVTENKGEIAAIIVEAIAGNMGCVLPKKGFLEDLRELCDKEEILLIFDEVMSGFRLGKGGAQEYFGVKADLITFGKVIGGGMPVGAYAGSSKIMDIVSPVGPVYQAGTLSGNPIAMIAGYTLLKELNQNPQIYTELADKTEYLHKGLDKVFTESGFDYKINRCGSMISIFFTDIDVVDFDTAATANNEKFPKFFHEMLKRGIYLPPSSFESYFLSNSLTYEMLDKTIQAAKESLEAMK
>NZ_AP018042.1|WP_096432373.1|5052964_5053669_-|uroporphyrinogen-III-synthase
MPKADNKHILFTRSLNSEHLKYAAELNLSITAKAFIQIDILSLSEAKIELINSKPESNWIFTSQNAVKVISKSLSSFQSLKEKKVFAVGDRTAEELLKIGIKAFLPEQHNSESLIELLEKQTVSSYIHFSGNLRQNIISDFMSEKELDFEEIECYQTKLHQPNINVDDFDAICFCSPSAVTSFFSKYRIKDTVPCIAIGSTTAVKLLDFSEHVVMSERTDVFSLLEICHNYLNS
>NZ_AP018042.1|WP_096432375.1|5053978_5056180_+|bifunctional-(p)ppGpp-synthetase/guanosine-3',5'-bis(diphosphate)-3'-pyrophosphohydrolase
MTEFNKEEEERRQILYRYKHLLVTCKNIITSTDIKLVRKAFELSLQDHDGERRTNGEPAIYKSISIAQIVVEELGLGRTSVICSLLYDIVQKNRITLDEVSNLFGPKVTQIIQGLVKVTNIYNQNPTLQSENFRKLLLSFADDVRVVLIIMADRLFNLRNLENKSKKIQQDLAKEIEFLYIPFAHRLGMYNVKSDMEDLALQYLDPDVYFEIEKKLKDSEEEREKYIKDFVNPIKAELDQFGMKYKIKARTKSIASILHKMKTKQVEFEEVYDIFAIRIILKAIPKKEKSECWKVYSTVSDLYQPNPNRLRDWITIPKSNGYESLHTTVMGPDNRWVEVQIRSKRMNQIAEQGFAAHWKYKGGKGEGGLDTWLQDIREVIESHGSDSIDMMDSFKADLYHKEIYVFTPKGDLKQLSAGATLLDFAYSIHSDVGDKCIGGTVNQKNVSIKQVLNNGDQVSITTSNSQKPKSDWLSFVVSTKAKNKIRQSIKEELLKEADLGKETLKRRMKNWKIEFCDENIRKLMTYYKYKLAVDLYQGIASEKHELSEIKDILTDKKEVEKIVPEHKTDYSTKKVTKGGEDVLIVDENISNVEYSLANCCNPIFGDEIFGFISIGKGIRIHRLGCPNAREMQTRYPYRVVKANWTDKGSASYQAVLHITGDDDLGIVSNISDVISKDLRVQMRSISVDTNAGSFAGNVAVVIASTDHLDTLIVKLKRVKGVRRVTRYDPIGEQ
>NZ_AP018042.1|WP_096432377.1|5056394_5058173_+|DNA-polymerase-III-subunit-gamma/tau
MENYIVSARKYRPSIFSSVVGQQSITVTLKNAIKNNHLAHAYLFCGPRGVGKTSCARIFAKTINCSNISSDFEACDECESCVAFNESRSYNIHELDAASNNSVEDIRTLIDQVRIPPQIGSYSVYIIDEVHMLSASAFNAFLKTLEEPPAHAIFVLATTEKHKILPTILSRCQIFDFNRIKVEDAVKHLKEIAIKESVIIEEEGLNVIAQKADGAMRDALSIFDQIVSFSGKEISFENVIQNLNVLDYDYYFKMVDAFLDGKVTDVLLMLNEIIEKGFDGHHFVAGLSAHIRDVLVGKDPVTIQLLEVSDSVKQKYVDQSKKCPLDFLYEALKILNQCDISFKGSQNQRLLIELSLIQLSQIIELKKKGLKLNLNSKKLQKIIVEGEAISAVVKQPEPQGNNGKATQTVSDTGQTYNSKKEKSKGVTVSPKSNFASSVSIKQAMNSGNTRSTNAAKPIDKKAISFAEVVVVGNEPITQEKVEAKLKEYIIAKGKSDRIRLAITVNKPKILDSNRVVLMVSNPLQEDDVIAVKNEVRNYLKKELNNSEIQIETEIIQIITEKRMYTDSDRFNYLCEKNPALGLLKHKFALDFE
>NZ_AP018042.1|WP_096432378.1|5058419_5060645_+|NADP-dependent-isocitrate-dehydrogenase
MAQKSKIIYTKIDEAPALATYSLLPIIKAFTKTSGIEIETKDISLPGRILANFPENLTEKQRVSDYLAELGELSLTPDANIIKLPNISASIPQLQAAIKELQEKGYNIPQYPEEAKTEEDKALQVRFAKVLGSAVNPVLREGNSDRRAAGSVKQYAKNNPHKMGKWSPDSKSHIAFMTEGDFYGTEKSVTMANPTNVRIEYVNTAGNVSVLKEKLELLAGEVIDSSVMNVKALRAFYAKEIAAAKNEGLLWSLHLKATMMKISDPVMFKHAVEVFYEKAISKHADTIKKLGVNFNNGLGDLYAKIEKLPEAKKKEIEADIMEVYKSRPDIAMVDSDKGITNLHVPNSVIIDASMPVVVRDGGKMWNATGKLQDTKALIPDRCYATIYQVCFDDCRKNGAYDVATMGSVSNVGLMAKKAEEYGSHDKTFIAQGEGTIRIVEFNGTVIMDQRVEEGDIFRMCQAKDAPIQDWVKLAVNRAKATNTPAIFWLDKNRAHDAQLITKVNTYLAEYDTKGLDIRILAPDDAIAFSLERIRKGEDTISVTGNVMRDYLTDLFPILELGSSSKMLSIVPLMNGGGLFETGAGGSAPKHVQQFLTEGHLRWDSLGEYLALGVSLEHLANKFNNEKAALFAETLDLGVSKFLEEKKSPSRKVNELDNRGSNFYLAMYWAEALAAQNKNAELKKTFVKVAADLKANEAKIVAELNDAQGKPMNIGGYFMPNEELVTKAMRPSVTLNTIIDAI
>NZ_AP018042.1|WP_096432380.1|5060753_5062352_-|cation:proton-antiporter
MDPVWLAIAFALGLLVKLIGLPPLVGYLIAGFTLKYFGAESDDLIRTISDMGITLLLFTIGLKLRIKDLLHKEVWGGASIHMLLVTLLMALILFGLSYTSLHIFTDFTWQQALIIGFALSFSSTIYAVKILEEKSELKSAYGVLSIGILIIQDIFAVFYIVLVAGKLPTIWAIGLPVLFVIIRPVLLLILDKIGHGEILVLYGFFLAFVVGAELFTVVGLKADLGALVVGILISNHKKAKELADSLMNFKDIFLIGFFLSIGLIGLPSLQMLTLAIVLALAINFKVILYFWVLTRFRVRARTSLFTSLTLANFSEFGLIVASIAVAKGLIPSDWLVSIAIAVATTFIISSPLNAKAHKIYYSLKDHLRKFETQKRLIYDKAFDIGNAEILVFGMGKLGLSVYDQLNKTYGRKVLGLDYNYDVVQQLKKDGKNIQQDDATDSEFWENIFEKPDSHKVRLVMLCMNDHKSNIFAVERLKATSFKGNIAAIARFDDERKQLEKMNVDAVYDIYSEAGYGFANHVCHQIDIGCTAS
>NZ_AP018042.1|WP_096432382.1|5062606_5063119_+|ferritin
MAISKKVEEILNKQINAEFWSAYFYLSMSNYCNANGMPGVANWMKVQFQEETSHAMKILDYVNERGGRVVLNPITEVPADWNGILHIFEETLKHEEIVTGLINGCVNVAIEEKDHASVNFLQWFVDEQVEEEATVNEILDQLRMFEGKGHGLYMMDKEFKARVFVDSTQA
>NZ_AP018042.1|WP_145957691.1|5063222_5063606_+|hypothetical-protein
MNRYLIVVFCVLTGCAAAVPMANGQEMSVDHHLFAEWYADHIQNLDESYSSTILAHVEQDQLVGKYIPSIENFSLLENLPKFPVADQVIPLESIPISQTLQCFSILPEVILSTIPEGDFHIRAPVLA

Crispr_ID: NZ_AP018042_13

• CRISPR_ID: NZ_AP018042_13
• CRISPR_location: 5644774-5644886
• CRISPR_type: Unclear
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: cas3

Consensus repeat of NZ_AP018042_13

Consensus_repeat	Method
GAGAGTCGCGCTCGAGTCTTTTTGCTCCG	CRISPRCasFinder

spacers of NZ_AP018042_13

>13.1|5644803|55|NZ_AP018042|CRISPRCasFinder
GCCTCGGGTCTTTTTTGTCCGGCGAGCTTATTTTGAAGTACTCCCCCCTACCTCA

CRISPR arrays and Neighbor proteins around NZ_AP018042_13

CRISPR arrays

The CRISPR arrays of NZ_AP018042_13

>merge|NZ_AP018042|13|5644774-5644886|CRISPRCasFinder
GAGAGTCGCGCTCGAGTCTTTTTGCTCCGGCCTCGGGTCTTTTTTGTCCGGCGAGCTTATTTTGAAGTACTCCCCCCTACCTCAGAGAGTCGAGCTCGAGTCTTTTTGCTCCG

>NZ_AP018042|13|12|5644774-5644886|CRISPRCasFinder
GAGAGTCGCGCTCGAGTCTTTTTGCTCCG	GCCTCGGGTCTTTTTTGTCCGGCGAGCTTATTTTGAAGTACTCCCCCCTACCTCA
GAGAGTCGAGCTCGAGTCTTTTTGCTCCG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP018042.1|WP_096433208.1|5649483_5653245_+|efflux-RND-transporter-permease-subunit	unknown	unknown	gnl|CDD|226220
NZ_AP018042.1|WP_096433214.1|5656661_5656997_+|heavy-metal-associated-domain-containing-protein	unknown	unknown	gnl|CDD|225328
NZ_AP018042.1|WP_096433202.1|5645664_5646786_-|DEAD/DEAH-box-helicase	cas3	cd09639_cas3_CAS-I	gnl|CDD|223587
NZ_AP018042.1|WP_096433218.1|5658251_5658635_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096433192.1|5635234_5635810_-|sigma-70-family-RNA-polymerase-sigma-factor	unknown	unknown	gnl|CDD|236608
NZ_AP018042.1|WP_162845518.1|5634224_5634758_-|periplasmic-heavy-metal-sensor	unknown	unknown	gnl|CDD|379377
NZ_AP018042.1|WP_096433194.1|5637086_5639891_+|DNA2/NAM7-family-helicase	unknown	unknown	gnl|CDD|350195
NZ_AP018042.1|WP_096433200.1|5641344_5644185_-|DUF4297-domain-containing-protein	unknown	unknown	gnl|CDD|366375
NZ_AP018042.1|WP_096433184.1|5632916_5633660_-|DUF2202-domain-containing-protein	unknown	unknown	gnl|CDD|313233
NZ_AP018042.1|WP_096433190.1|5634750_5635188_-|zf-HC2-domain-containing-protein	unknown	unknown	gnl|CDD|379219
NZ_AP018042.1|WP_096433212.1|5654796_5656584_+|efflux-RND-transporter-periplasmic-adaptor-subunit	unknown	unknown	gnl|CDD|379862
NZ_AP018042.1|WP_096433196.1|5640129_5640792_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096433186.1|5633696_5634137_-|hypothetical-protein	unknown	unknown	gnl|CDD|197366
NZ_AP018042.1|WP_096433220.1|5658979_5659714_+|peroxiredoxin	unknown	unknown	gnl|CDD|237297
NZ_AP018042.1|WP_096433840.1|5636244_5637042_+|alpha/beta-fold-hydrolase	unknown	unknown	gnl|CDD|378820
NZ_AP018042.1|WP_096433204.1|5647779_5648256_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_096433198.1|5640791_5641268_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP018042.1|WP_162845519.1|5653415_5654651_+|TolC-family-protein	unknown	unknown	gnl|CDD|224455
NZ_AP018042.1|WP_096433216.1|5657317_5658109_+|DUF4382-domain-containing-protein	unknown	unknown	gnl|CDD|379555
NZ_AP018042.1|WP_096433206.1|5648872_5649247_+|hypothetical-protein	unknown	unknown	unknown

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_AP018042.1|WP_096433202.1|5645664_5646786_-|DEAD/DEAH-box-helicase	cas3	cd09639_cas3_CAS-I	2.9e-12	41.0	6.4

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP018042.1|WP_096433214.1|5656661_5656997_+|heavy-metal-associated-domain-containing-protein	gnl|CDD|225328	COG2608, CopZ, Copper chaperone [Inorganic ion transport and metabolism].	8.29259e-09
NZ_AP018042.1|WP_096433202.1|5645664_5646786_-|DEAD/DEAH-box-helicase	gnl|CDD|223587	COG0513, SrmB, Superfamily II DNA and RNA helicases [DNA replication, recombination, and repair / Transcription / Translation, ribosomal structure and biogenesis].	6.78665e-128
NZ_AP018042.1|WP_096433192.1|5635234_5635810_-|sigma-70-family-RNA-polymerase-sigma-factor	gnl|CDD|236608	PRK09652, PRK09652, RNA polymerase sigma factor RpoE; Provisional.	7.96862e-42
NZ_AP018042.1|WP_162845518.1|5634224_5634758_-|periplasmic-heavy-metal-sensor	gnl|CDD|379377	pfam13801, Metal_resist, Heavy-metal resistance. This is a metal-binding protein which is involved in resistance to heavy-metal ions. The protein forms a four-helix hooked hairpin, consisting of two long alpha helices each flanked by a shorter alpha helix. It binds a metal ion in a type-2 like centre. It contains two copies of an LTXXQ motif.	0.000496613
NZ_AP018042.1|WP_096433194.1|5637086_5639891_+|DNA2/NAM7-family-helicase	gnl|CDD|350195	cd18808, SF1_C_Upf1, C-terminal helicase domain of Upf1-like family helicases. The Upf1-like helicase family includes UPF1, HELZ, Mov10L1, Aquarius, IGHMBP2 (SMUBP2), and similar proteins. They are DEAD-like helicases belonging to superfamily (SF)1, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. Similar to SF2 helicases, SF1 helicases do not form toroidal structures like SF3-6 helicases. Their helicase core consists of two similar protein domains that resemble the fold of the recombination protein RecA. This model describes the C-terminal domain, also called HelicC.	1.48407e-29
NZ_AP018042.1|WP_096433200.1|5641344_5644185_-|DUF4297-domain-containing-protein	gnl|CDD|366375	pfam00931, NB-ARC, NB-ARC domain.	0.000128808
NZ_AP018042.1|WP_096433184.1|5632916_5633660_-|DUF2202-domain-containing-protein	gnl|CDD|313233	pfam09968, DUF2202, Uncharacterized protein domain (DUF2202). This domain, found in various hypothetical archaeal proteins, has no known function.	1.39467e-57
NZ_AP018042.1|WP_096433190.1|5634750_5635188_-|zf-HC2-domain-containing-protein	gnl|CDD|379219	pfam13490, zf-HC2, Putative zinc-finger. This is a putative zinc-finger found in some anti-sigma factor proteins.	1.94747e-05
NZ_AP018042.1|WP_096433186.1|5633696_5634137_-|hypothetical-protein	gnl|CDD|197366	cd09916, CpxP_like, CpxP component of the bacterial Cpx-two-component system and related proteins. This family summarizes bacterial proteins related to CpxP, a periplasmic protein that forms part of a two-component system which acts as a global modulator of cell-envelope stress in gram-negative bacteria. CpxP aids in combating extracytoplasmic protein-mediated toxicity, and may also be involved in the response to alkaline pH. Functioning as a dimer, it inhibits activation of the kinase CpxA, but also plays a vital role in the quality control system of P pili. It has been suggested that CpxP directly interacts with CpxA via its concave polar surface. Another member of this family, Spy, is also a periplasmic protein that may be involved in the response to stress. The homology between CpxP and Spy suggests similar functions. A characteristic 5-residue sequence motif LTXXQ is found repeated twice in many members of this family.	8.10111e-06
NZ_AP018042.1|WP_096433220.1|5658979_5659714_+|peroxiredoxin	gnl|CDD|237297	PRK13189, PRK13189, peroxiredoxin; Provisional.	2.69625e-131
NZ_AP018042.1|WP_096433840.1|5636244_5637042_+|alpha/beta-fold-hydrolase	gnl|CDD|378820	pfam12146, Hydrolase_4, Serine aminopeptidase, S33. This domain is found in bacteria and eukaryotes and is approximately 110 amino acids in length. It is found in association with pfam00561. The majority of the members in this family carry the exopeptidase active-site residues of Ser-122, Asp-239 and His-269 as in UniProtKB:Q7ZWC2.	1.47404e-19
NZ_AP018042.1|WP_096433212.1|5654796_5656584_+|efflux-RND-transporter-periplasmic-adaptor-subunit	gnl|CDD|379862	pfam16576, HlyD_D23, Barrel-sandwich domain of CusB or HlyD membrane-fusion. HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.	1.66394e-87
NZ_AP018042.1|WP_096433208.1|5649483_5653245_+|efflux-RND-transporter-permease-subunit	gnl|CDD|226220	COG3696, COG3696, Putative silver efflux pump [Inorganic ion transport and metabolism].	3.01521e-160
NZ_AP018042.1|WP_162845519.1|5653415_5654651_+|TolC-family-protein	gnl|CDD|224455	COG1538, TolC, Outer membrane protein [Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion].	1.64833e-12
NZ_AP018042.1|WP_096433216.1|5657317_5658109_+|DUF4382-domain-containing-protein	gnl|CDD|379555	pfam14321, DUF4382, Domain of unknown function (DUF4382). This family is found in bacteria and archaea, and is typically between 142 and 161 amino acids in length.	9.52667e-41

Protein sequences

>NZ_AP018042.1|WP_096433200.1|5641344_5644185_-|DUF4297-domain-containing-protein
MIDKDYIKIFDINTDAVAVNRGFYYQYLITLKKWIQNYIDKKNTFVYCEVDDDIKEVGNSLIFTQVKCYSRNFSLKSDEIKKTLFNFFIQFLKNKQDIPIGFCFTTNTGLVKSEKLIKAWMANPELTDSELANQILKKVKEILNSEINKNKRKKTEKKTIVRKEIDLIKVAAEQLKKQVAYLSIDSFVKYIKWEFLDYDPILAIDLLFEEVRQLLSHEIFKNKPVSIIENILLSEIFRCSQLQDSNKRVLSNSRIDEILNNTDSDINRFVDDRLISLLGVKFEEIEQNFKNIQRVQNSFEKRLDSFDDRIILNDTSYPKSLTLIPKSLSQDFNDSQESIDYVIKKLLETNHLCINGNGGVGKTFFAKSFVSQINEQYDHIAWINSSPNLTKSILLNAALKQNLKLDFNNQTNDEERIDIVCSELQRIDGKNLLIIDNYENDFRALKKIISLDNWKILITTRERVPNLLNYTLPGIDKEIASKIFCNYSNEEIDSDNSILLEFFEYVNYNPLIIKLCGKTIANSIDLNLESLYSSIKEQKLDNESLEIEIDLSEEDLPHTILAYLYKTFELKNLTKNEEIYLEFLALLPSEEVSIKDIALIGGKESYNKNLKDFTNWTNSLHKKGWIERVNGEVRMYRLVQELIIYKTRKQQNGFISNVLLFNWLFHRIDEVAQSDPTLSFPFLKYAESILKAIKEEYRQSIYQPLLLLENALLNSYTWIENTSALHQRWIDLVKRAELYLPNDDVNLGIMYNNLGYSYARRNDIETAVDYFKKSIFTLTKQEEESINVLINSLNNLTQAYLVLENLPSADKIQKKTRQLLKKYSLTTKQFEAVSYFLRAVFLMKSENFRGAIEQYELAIQTHLKIEKDSRNDFLLLMYYSNLILMLFKAKQSDKVIENIDNIREIIETHKMSYSSVVNEVKIMIDNINDFYQKGVKQQVPTKAYKS
>NZ_AP018042.1|WP_096433198.1|5640791_5641268_-|hypothetical-protein
MKTLISDYINVREKYQDENWDLKEEIAFIPSNLELETNVYFYANSVKSLKTVFRNNDLSFDVVDNKEIGYQQLNSFEWIGPTLFFGASILSENQHLISISLSVIASYLTDFFRGISGDKKVKLDIVVEKEKNKTYKRIQFEGSVDSLKDLVDVIKAVK
>NZ_AP018042.1|WP_096433196.1|5640129_5640792_-|hypothetical-protein
MEKDLEKFQDNFDEVIHSCQRFTPFVRGIEFQKESVDTLDKLIDTIRQNKVIAIEAQKEEIANILLSMEQMAISAKSEIEMVILIKEDKINEAWDKLIDAQMSMRGAMQAHPTGTTHLEPYVDKLIAYEKLLFPNQLFMSTGLIAGAGECSICGSEYGTCNHLKGKAYMGKFCHEIIKEVKQLNEVSIVEDPASKRCRAYTITGDGKNRDIMTWRESKSE
>NZ_AP018042.1|WP_096433194.1|5637086_5639891_+|DNA2/NAM7-family-helicase
MPDTINNLIQYYHDCYQSDNRELIIYNFLDKKVENKIYFEGKEELLTDSNPLIPIDSVKASAICKKTKLFQKEKELLYGTFFICGNYTDFKGDSKDLCAPLFYYPAEIKQKDDFFYLSISASERRLNYPIIQMLSKESSGELLQDPLFEKLPTDFIRFEHIESIVLLFKKYFPNVNIADIYSYPDNTSITTVKKTISKLSKEKQDEYVLLPASILGLVAKSSNTRGVLNELSEMASMTDHSFPLLSLLSKSAPKEKLKKYEKGNLPMILSEAQQAILKSSSINPLTLIVGPPGTGKTYTISAIALEHMSRGESVLIASRTDEAVDVIVEKIKNQLGIDKAVVRTGKKRSYSTPLNRFLKSLLTRVNKLSFLLKEFDLPKTKMDELEKKVSSLSSTIESRSKLIEKLESSFSKEVENELKWGEHLSKEKAGVWNKIKTQYIDIRNKIQIPIWEYNSKLNQNDLCQITDVQLLIRLKYVYQILKVMTADWKNIQRFHEALKMSSDTEKLAKFRDINFDAVLKAFPIWLTKLSEVKDALPFEKELFDVVIIDEATQCDIASCLPLLQRAKRVVFAGDPNQLRHVSFLSKGIQAILRSKYELQDVEYSTLNYRDKSILDLAMSSLQSSDQVAMLDEHFRSLSPIIAFSNEQFYDNQLKIMTSRPDETEEALYFINNHGIRNKNGANEIEANAILKAVHELVKNEKDLNENLCSSVGILSPFRAQVDWMSKLLLEQFEMDEIEKHKIRVGTAYSFQGEERDVMHLSFAVDGNSHHSAINHINKEDVFNVAITRARTRQYIHHSLSKNELKADSLLRSYLSKPALHEFQADKGTTPHDVYLDEVKNVLKNWDINKYWIGYSIAGLTIDLLVKYKDKYLGIDLVGYPGEYADVFGIERYRILNRAGIKVFPLPYSDWHFENEKSKKVLKEFFLNLKGASVN
>NZ_AP018042.1|WP_096433840.1|5636244_5637042_+|alpha/beta-fold-hydrolase
MRKLLLRTLTILCFFTAVGTTLLYFNQEKLIFFPQKLDKEYRYSSDMSFEEITIETEDHKNLNGLLFTVENSKGLVFYLHGNAGSLTNCGDVAKTYTDLNYDIFMLDYRGFGKSEGDIDSQQQIFTDIQTAYTLILKRYQEDKVIVLGYSIGTGLATHLAATNKPRLLVLQAPYYSLTDMMKRRYKYLPAVLLKYKFPTNELITACTMPIVIFHGTQDRIIPYESSLMLQKISKSSDQLITLKDVGHGGINSNPKFKTEIARILN
>NZ_AP018042.1|WP_096433192.1|5635234_5635810_-|sigma-70-family-RNA-polymerase-sigma-factor
MIERELVEQLKSGNETAFRSLVETYQTMVVNTAMGMIHNQADAEDIAQEVFMQVYHSITDFRSESSLKTWLYRITITRSLNAIRDRKRRNFFSRVEDFFQGKKEEVNEKASFVNPDKALEEKDHANAIHTALDALPENQKIAFTLSKYEELSYEEIAELMQLSKSSVESLLFRAKSNLQKKLIDYYKNFTK
>NZ_AP018042.1|WP_096433190.1|5634750_5635188_-|zf-HC2-domain-containing-protein
MECKEVNSKLIFYLEKELLKDEMLQMENHLSNCSSCSDSLVLLQSALGIIEKEKTLQANPYFVSKTMNRFKEEQRKANQPVFTRLLMPVLVSMLFVLAIGSGIFMGSNFATDTENIQQSNLIDPYFNEIDNETIELFFLNDGYHE
>NZ_AP018042.1|WP_162845518.1|5634224_5634758_-|periplasmic-heavy-metal-sensor
MNKANKFWMWLALLLLAVNLSTIGSLWYHMQQETKEVENKVEMPDEFRTRFLKEQLGLDEEMKIKFRSLNRNYNQEANQIMNQLNGLRRDMVQQLGNENCDSLELNNIAGLIGEKHKELKLATVQFYLDMKSELNEEQQAKLYDLFSSLLQKDEEVKTPKGRRKGWGHGNGPGGRNQ
>NZ_AP018042.1|WP_096433186.1|5633696_5634137_-|hypothetical-protein
MKTQNLIKNLGLLLMVIMFAIPAADSFAQNRRRANDCSQISNLTVEQNKQLNDLRTDHLKVMDGLRADRRAAVNESDRANVIEQMRAELTSHTNAVSAVLTPEQQAQYQQNKNSNAQCFSGKGRRGCGNGCGQGRRGGGRNGRGNW
>NZ_AP018042.1|WP_096433184.1|5632916_5633660_-|DUF2202-domain-containing-protein
MRNKITKVLIAFTLMLGFVACDNEDAGTDALANLPLVEVAEDGSTDMLAGNLSAVLDPELTTFTDSEQDGLLLMREEELLAHDVYSLFSDLYDVKTFSNITGAESTHAAAVLTLLDLFEIEDPSTGIAGEYQNETLQALYDELSEKGMVSVEEALKVGALIEEVDIADLEELMAETENENILLVYSNLLRGSRNHLRAFVRVLGTYGVDYLPTVLSEDDFNEIINSDTERGTGCLNEYQKNRYGNNN
>NZ_AP018042.1|WP_096433202.1|5645664_5646786_-|DEAD/DEAH-box-helicase
MENFKELGVDKDIIKGLNDLNIIKPTQIQAEVISVLLQANTDLIGQAQTGTGKTAAFGIPLLHRINTKADKVQGLILCPTRELGQQIAKQLFKFTKYTDQIFTESVYGGEKIEKQISALRRPTHIIVATPGRLIDLVKRKAVDLSHVKTVILDEADEMMSMGFKTQLDEILTHLTSVENRWLFSATMPKEIMQIVKRHFSPRAHKIEVSGKNVVNKNIQHQFLICEDNEKLNILVQFLKSEQDNRGVVFCKTKASAQVLAKQLIAKNIKTDAIHGDLKQIERDKVMRAFKNESLRILVATDVAARGIDIVDLAFVVHYELPDKEEYYTHRSGRTARAGKQGVSLSLVNSKELKNLRYFQKTLNIAFNQIRPRK
>NZ_AP018042.1|WP_096433204.1|5647779_5648256_-|hypothetical-protein
MKNLQQLVQWTRIGLVSAFLLVVIVELALGGLAISGEYSILSIPKSLNISMWKNVLGLSLIIVEIVISEMMYKKKLEYVALFESLEDKLKYFKKAFSFKMLFGALIGIVSLLAYSLTENMIWFLPWIMVLYTLSKSFPFKWTLVHAMQIENEEDRELF
>NZ_AP018042.1|WP_096433206.1|5648872_5649247_+|hypothetical-protein
MRIKKFEHIIMILLLLISTAGVSINKHYSGGELFSTAFFVEAESCCETPCGCCKETSEFVQVKADYIDSVFELQDAAQLDLLFSILPFLINFEVPVSFTNNFIAWDTSPHPLPDLCILNQVFRL
>NZ_AP018042.1|WP_096433208.1|5649483_5653245_+|efflux-RND-transporter-permease-subunit
MLNKIIKFFLENKLVTTILLVVFIAWGVITSPFPWDVSFLPKDPVSVDAIPDIGENQQIVFTKWPGRSPQDIEDQITYPLTTSLLGIPGVKTIRSSSMFGFSSIYIIFNEDIEFYWSRSRILEKLSSLPQNILPDDVQPTLGPDATALGQIFWYTLEGRSPEGEVTGGWDLQELRSIQDFQVKYALAAASGVSEVASIGGYVKEYQVDVNPAAMQAHGVNLMQIMNAVKKSNMDIGARTIEINQAEYFVRGIGYVKNLSDLEEAVVSVNDNTPVRVRDVANVHYGPSDRRGVLDKMGAEVVGGVVVARYGANPLEVINSVKEKIADLSVGLPEKELADGTISKLTIVPFYDRTQLIYETLGTLESALTLEIIITIIVIIIMVMNLRASILISSLIPIAVLMTFIAMRYFHVDANIVALSGIAIAIGTIVDVGIVLSENMIRHLDENKSGRNIREVIYEATTEVAPAVITAVLTTIVSFIPVFAMEAAEGKLFRPLAFTKTFVLLSALFVAIVILPAMAHLLFSIRIKKRNFKLALNSVLILIGCYAIFAYSAWIGITLILLGVINLLAQIYSKYEKKLSLANLLVIALAVSFLLAREWLPLGAANSLFANFVFVILSIAILLSIFLLFMRFYQRMLNWALQHKLIFLSIPTFIVLWGIIIWLGFGNTFGFVARGMDKIGIEISGTSAWKSMNHNYPGIGKEFMPSLDEGSFLLMPTSMPHAGIEENKRVLRKLDMAVANIPEIEMVVGKLGRVESSLDPAPISMYENVINYKSEYKTNQYGKRIRFAVDEDDNFIRDEKGELIPDDSGEYYRQWRDHIKSPDDIWTEIVNATKIPGVTSAPKLQPIETRLIMLQTGMRAPIGIKISGNNLKQIEGFGLQLEKLIKEVPGVNKPSVFAERMVGKPYLEIRIDRKAIARYGIQVEDVQKHLSVAVGGMALSSSVEGRERYPIRVRYPRELRSDPELLQKILIPTAMGNSVPLEELAEIAYVQGPQVIKSENTFQVGYVIFDKLDGFSEVDIVEAAQEYIAGKIESNELKVPAGVSFKFTGNYENQIRAEKRLGIVIPVVLIIIFLILYFQFKGIAPTLMVFSGIAVSFAGGFIMIWMYGQGWFLNFSVFDINLQELFQVHPIYLSVAVWVGFIALFGIASDDGVIMASYLEQVFEKNQPKTKAEIRKAVVEAGSKRIRPCLMTTATTLLALLPILTSTGRGSDVMIPMAIPAFGGMTIELITLFVVPVLYATYKEYQLKKVGGLS
>NZ_AP018042.1|WP_162845519.1|5653415_5654651_+|TolC-family-protein
MRYSILLITALLVFSETVRSQTITKELQDYINFAIANNPELKVMDLKYQQALEIVPQAKALPDPSFSAGVFIQPIETRIGAQKAKLSLSQMFPWFGTLGAKEQQASLQAHAQYLNYLDAKSKLEMNVRLSYLDLILMDRKIFFTQKRVEVLSLLEKQSLSQFENNQSSMVNVLYVQMLKEELISKLGLFQDKKQTLQSTFNKLLNRNLITAVDLPTDDSFLAQAISFDTDFSFDNHTRIDSWETMKEASSFGEKVAKLNGMPKFGIGLDYAIIAERTDMDVANNGNDALMPMITMSIPLFRKKYKSAVKVNQLKQVQFEQLKIEELNRLELEKQKATEEYATGTREIKVYENLLAKARQSFEILTSNYETSSGKYEEVLNMQQKIWIYEQKQIEAQVMIQKSIAKFQYLGM
>NZ_AP018042.1|WP_096433212.1|5654796_5656584_+|efflux-RND-transporter-periplasmic-adaptor-subunit
MKNTIKYISENWKQKLIILVVGLFLGWIIFGGKSESTNEHEGHQHASENAAVAPTIWTCSMHPQIQQPSEGDCPICGMDLIPLDNSSSNSSPAIIEMNETAMKLANVQTTKIEKGKAVKEILLNGKVQVDERKISSQAIHFDGRIEKLMVNFEGEKVREGQTLARVYAPKLVSAQQELLQSLKTKDSYPELVKAAEQKLKFWKLSADQINSIKESGEVLQYFEIKADASGYVIKRNVAEGNYAKAGSILFEIADLSHVWVVFDAYEKDLAFIKKGAEIEFTISAIPGKTFNSKVSNIDPVIDAKKRTLSIRTELNNQDLLVKPEMFASGIIHAEINQMNNALLVPKSAIMWTGKRSVSYVKVDGGFEMREIVLGESLGDFYIVTDGLEENEEVVSKGTFTVDAAAQLNNKYSMMNRPESKKGVPNLQGYASMDFSMKLEQLLSSYFALKDILVETKAKEGKIAAKMVMSNLMKMMPNDAALKGKAVEFWKEKYPVLHDHLVEIMNSDDIKIQRKAFKPFSEALIGTLKSLGAGKNKVFIQFCPMADSDSGAFWLSSEEKIMNPYFGDMMLHCGEVTDTIIHEEPKSNPQQHQHQH
>NZ_AP018042.1|WP_096433214.1|5656661_5656997_+|heavy-metal-associated-domain-containing-protein
MKKGILFTAVIFSMITISFTANAEVKKASFKVSGNCGMCEKTIETAANAVEGVVSADWNKESKEMIVSFDTEKTNLDAVHKAIAKSGYDTDTVKADDKVYSGLNSCCKYRK
>NZ_AP018042.1|WP_096433216.1|5657317_5658109_+|DUF4382-domain-containing-protein
MKILKSLSAITLVILSLIFIQCSDDNVNLTTPVKIRLTDAPAQYDKVNIDIQAIQFHSSNDDTKESGWQEMDLLNAGIYDLLEFNNGLDTLLVDQDMPSGTVSQMRLILGENNSVVIDGVPYVLDTPSAQTSGLKFQIHDDFIAGIEYELWIDFDAARSIVETGNGKYKLKPVIRTFNEATSGVISGSINPAEALPTIHAIIGLDTTSTIAEANGDFMIKGLQSGIYKLDLMPIDGYTEKEIEGIEVNNGAVTDAGTIEILAN
>NZ_AP018042.1|WP_096433218.1|5658251_5658635_+|hypothetical-protein
MKFKYKISPDLKLINEYYYGYFKWNDLINNTLSTRENSLFDETYNVIADFTQAKVILNIKDLDDYIEEYQKYSLIANKCAIIVSESYDTSMAMLFEIQVRIQNAKVFHSHEEACEWLEIDPVLITKE
>NZ_AP018042.1|WP_096433220.1|5658979_5659714_+|peroxiredoxin
MEELKEITLASMPRIGDKAPEFRAVTTQGNIHFPNDYQGSWVILFSHPADFTPVCTSEFMTFASLESKFNEANCELVGLSIDGLYSHIAWLRTIKEKIEYKGMKNVEVKFPLIEDITMEVANKYGMIQPGEDNTKAVRAVFFIDPHGIIRTIIYYPLSLGRNFDEIYRVVIALQTADKFSVATPADWQPGDEVIVPPAGSCGVAKDRMENTDEDVHCYDWFFCTKKIEKEEILNAILKKEELSH

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_AP018042_1	1.1|338276|20|NZ_AP018042|CRISPRCasFinder	338276-338295	20	NZ_AP018042.1	337574-337593	0	1.0
NZ_AP018042_1	1.1|338276|20|NZ_AP018042|CRISPRCasFinder	338276-338295	20	NZ_AP018042.1	338314-338333	0	1.0
NZ_AP018042_2	2.1|338694|15|NZ_AP018042|CRISPRCasFinder	338694-338708	15	NZ_AP018042.1	339069-339083	0	1.0
NZ_AP018042_7	7.14|3857653|32|NZ_AP018042|CRT	3857653-3857684	32	NZ_AP018042.1	3944870-3944901	0	1.0
NZ_AP018042_7	7.15|3857718|32|NZ_AP018042|CRT	3857718-3857749	32	NZ_AP018042.1	3944870-3944901	0	1.0
NZ_AP018042_7	7.17|3857847|32|NZ_AP018042|CRT	3857847-3857878	32	NZ_AP018042.1	3944870-3944901	0	1.0
NZ_AP018042_1	1.1|338276|20|NZ_AP018042|CRISPRCasFinder	338276-338295	20	NZ_AP018042.1	339033-339052	1	0.95
NZ_AP018042_7	7.11|3857456|32|NZ_AP018042|CRT	3857456-3857487	32	NZ_AP018042.1	3944869-3944900	1	0.969
NZ_AP018042_7	7.18|3857912|32|NZ_AP018042|CRT	3857912-3857943	32	NZ_AP018042.1	4411297-4411328	1	0.969
NZ_AP018042_1	1.1|338276|20|NZ_AP018042|CRISPRCasFinder	338276-338295	20	NZ_AP018042.1	345868-345887	2	0.9
NZ_AP018042_1	1.1|338276|20|NZ_AP018042|CRISPRCasFinder	338276-338295	20	NZ_AP018042.1	346522-346541	2	0.9
NZ_AP018042_7	7.34|3857654|42|NZ_AP018042|CRISPRCasFinder	3857654-3857695	42	NZ_AP018042.1	3944871-3944912	2	0.952
NZ_AP018042_7	7.35|3857719|42|NZ_AP018042|CRISPRCasFinder	3857719-3857760	42	NZ_AP018042.1	3944871-3944912	2	0.952
NZ_AP018042_7	7.37|3857848|42|NZ_AP018042|CRISPRCasFinder	3857848-3857889	42	NZ_AP018042.1	3944871-3944912	2	0.952
NZ_AP018042_7	7.38|3857913|42|NZ_AP018042|CRISPRCasFinder	3857913-3857954	42	NZ_AP018042.1	3944871-3944912	2	0.952

1. spacer 1.1|338276|20|NZ_AP018042|CRISPRCasFinder matches to position: 337574-337593, mismatch: 0, identity: 1.0

ggacttttggactttggact	CRISPR spacer
ggacttttggactttggact	Protospacer
********************

2. spacer 1.1|338276|20|NZ_AP018042|CRISPRCasFinder matches to position: 338314-338333, mismatch: 0, identity: 1.0

ggacttttggactttggact	CRISPR spacer
ggacttttggactttggact	Protospacer
********************

3. spacer 2.1|338694|15|NZ_AP018042|CRISPRCasFinder matches to position: 339069-339083, mismatch: 0, identity: 1.0

ttgactttttgactt	CRISPR spacer
ttgactttttgactt	Protospacer
***************

4. spacer 7.14|3857653|32|NZ_AP018042|CRT matches to position: 3944870-3944901, mismatch: 0, identity: 1.0

catccattgccccgtgctttagctcggggttt	CRISPR spacer
catccattgccccgtgctttagctcggggttt	Protospacer
********************************

5. spacer 7.15|3857718|32|NZ_AP018042|CRT matches to position: 3944870-3944901, mismatch: 0, identity: 1.0

catccattgccccgtgctttagctcggggttt	CRISPR spacer
catccattgccccgtgctttagctcggggttt	Protospacer
********************************

6. spacer 7.17|3857847|32|NZ_AP018042|CRT matches to position: 3944870-3944901, mismatch: 0, identity: 1.0

catccattgccccgtgctttagctcggggttt	CRISPR spacer
catccattgccccgtgctttagctcggggttt	Protospacer
********************************

7. spacer 1.1|338276|20|NZ_AP018042|CRISPRCasFinder matches to position: 339033-339052, mismatch: 1, identity: 0.95

ggacttttggactttggact	CRISPR spacer
ggacttttggattttggact	Protospacer
***********.********

8. spacer 7.11|3857456|32|NZ_AP018042|CRT matches to position: 3944869-3944900, mismatch: 1, identity: 0.969

acattcattgccccgtgctttagctcggggtt	CRISPR spacer
acatccattgccccgtgctttagctcggggtt	Protospacer
****.***************************

9. spacer 7.18|3857912|32|NZ_AP018042|CRT matches to position: 4411297-4411328, mismatch: 1, identity: 0.969

tatccattgccccgtgctttagctcggggttt	CRISPR spacer
tatccattgccccgtgctttagctcgaggttt	Protospacer
**************************.*****

10. spacer 1.1|338276|20|NZ_AP018042|CRISPRCasFinder matches to position: 345868-345887, mismatch: 2, identity: 0.9

ggacttttggactttggact	CRISPR spacer
ggacttttcgactttcgact	Protospacer
******** ****** ****

11. spacer 1.1|338276|20|NZ_AP018042|CRISPRCasFinder matches to position: 346522-346541, mismatch: 2, identity: 0.9

ggacttttggactttggact	CRISPR spacer
ggacttttcgactttcgact	Protospacer
******** ****** ****

12. spacer 7.34|3857654|42|NZ_AP018042|CRISPRCasFinder matches to position: 3944871-3944912, mismatch: 2, identity: 0.952

atccattgccccgtgctttagctcggggttttgtgaaagttt	CRISPR spacer
atccattgccccgtgctttagctcggggttttgtgattgttt	Protospacer
************************************  ****

13. spacer 7.35|3857719|42|NZ_AP018042|CRISPRCasFinder matches to position: 3944871-3944912, mismatch: 2, identity: 0.952

atccattgccccgtgctttagctcggggttttgtgaaggttt	CRISPR spacer
atccattgccccgtgctttagctcggggttttgtgattgttt	Protospacer
************************************  ****

14. spacer 7.37|3857848|42|NZ_AP018042|CRISPRCasFinder matches to position: 3944871-3944912, mismatch: 2, identity: 0.952

atccattgccccgtgctttagctcggggttttgtgagagttt	CRISPR spacer
atccattgccccgtgctttagctcggggttttgtgattgttt	Protospacer
************************************  ****

15. spacer 7.38|3857913|42|NZ_AP018042|CRISPRCasFinder matches to position: 3944871-3944912, mismatch: 2, identity: 0.952

atccattgccccgtgctttagctcggggttttgtgaatattt	CRISPR spacer
atccattgccccgtgctttagctcggggttttgtgattgttt	Protospacer
************************************ *.***

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_AP018042_7	7.10|3857392|31|NZ_AP018042|CRT	3857392-3857422	31	ACSJ01000017	Clostridium phage D-1873 CLG.Contig173, whole genome shotgun sequence	44965-44995	6	0.806
NZ_AP018042_7	7.7|3857194|33|NZ_AP018042|CRT	3857194-3857226	33	MT121964	Phage DP SC_6_H4_2017 DNA cytosine methyltransferase and putative helicase genes, complete cds	64252-64284	7	0.788
NZ_AP018042_7	7.7|3857194|33|NZ_AP018042|CRT	3857194-3857226	33	NZ_AP014580	Burkholderia sp. RPE67 plasmid p2, complete sequence	382122-382154	7	0.788
NZ_AP018042_7	7.10|3857392|31|NZ_AP018042|CRT	3857392-3857422	31	MN694732	Marine virus AFVG_250M550, complete genome	20639-20669	7	0.774
NZ_AP018042_7	7.12|3857521|33|NZ_AP018042|CRT	3857521-3857553	33	NZ_CP040335	Bacillus cereus strain DLOU-Changhai plasmid unnamed1, complete sequence	19052-19084	7	0.788
NZ_AP018042_7	7.12|3857521|33|NZ_AP018042|CRT	3857521-3857553	33	NC_004821	Bacillus prophage phBC6A52, complete genome	4232-4264	7	0.788
NZ_AP018042_7	7.9|3857326|33|NZ_AP018042|CRT	3857326-3857358	33	NZ_CP042840	Enterococcus sp. DA9 plasmid unnamed4	56052-56084	8	0.758
NZ_AP018042_7	7.16|3857783|31|NZ_AP018042|CRT	3857783-3857813	31	NC_019777	Cyanobacterium aponinum PCC 10605 plasmid pCYAN10605.01, complete sequence	62584-62614	8	0.742
NZ_AP018042_7	7.7|3857194|33|NZ_AP018042|CRT	3857194-3857226	33	KC821607	Cellulophaga phage phi19:1, complete genome	25917-25949	9	0.727
NZ_AP018042_7	7.10|3857392|31|NZ_AP018042|CRT	3857392-3857422	31	NZ_AP017383	Moraxella osloensis strain KMC41 plasmid pMOSL2, complete sequence	46587-46617	9	0.71
NZ_AP018042_7	7.10|3857392|31|NZ_AP018042|CRT	3857392-3857422	31	NZ_CP024186	Moraxella osloensis strain TT16 plasmid p1, complete sequence	63766-63796	9	0.71
NZ_AP018042_7	7.10|3857392|31|NZ_AP018042|CRT	3857392-3857422	31	NZ_CP024177	Moraxella osloensis strain YHS plasmid pYHS1, complete sequence	63734-63764	9	0.71
NZ_AP018042_7	7.12|3857521|33|NZ_AP018042|CRT	3857521-3857553	33	NZ_LR214939	Mycoplasma salivarium strain NCTC10113 plasmid 2	228884-228916	9	0.727
NZ_AP018042_7	7.12|3857521|33|NZ_AP018042|CRT	3857521-3857553	33	KC465899	Pelagibacter phage HTVC008M, complete genome	98995-99027	9	0.727
NZ_AP018042_7	7.16|3857783|31|NZ_AP018042|CRT	3857783-3857813	31	NC_027643	Microviridae Fen685_11, complete genome	4543-4573	9	0.71
NZ_AP018042_7	7.5|3857062|33|NZ_AP018042|CRT	3857062-3857094	33	MN693292	Marine virus AFVG_25M2, complete genome	34856-34888	10	0.697
NZ_AP018042_7	7.12|3857521|33|NZ_AP018042|CRT	3857521-3857553	33	MT682709	Escherichia phage vB_EcoM_SP1, complete genome	42993-43025	10	0.697

1. spacer 7.10|3857392|31|NZ_AP018042|CRT matches to ACSJ01000017 (Clostridium phage D-1873 CLG.Contig173, whole genome shotgun sequence) position: , mismatch: 6, identity: 0.806

ttgtaattttttaaatctctaatca-tatcgg	CRISPR spacer
atttaattttttaaatctgttatcattatct-	Protospacer
 * *************** * **** ****  

2. spacer 7.7|3857194|33|NZ_AP018042|CRT matches to MT121964 (Phage DP SC_6_H4_2017 DNA cytosine methyltransferase and putative helicase genes, complete cds) position: , mismatch: 7, identity: 0.788

actttgatttttgcataaaagaaataaaatctg	CRISPR spacer
atgtttatatttgcataaaagaaataaatacta	Protospacer
*. ** ** *******************  **.

3. spacer 7.7|3857194|33|NZ_AP018042|CRT matches to NZ_AP014580 (Burkholderia sp. RPE67 plasmid p2, complete sequence) position: , mismatch: 7, identity: 0.788

actttgatttttgcataaaagaaataaaatctg	CRISPR spacer
aatttgattttcgcataaatgaaataaggcttg	Protospacer
* *********.******* *******....**

4. spacer 7.10|3857392|31|NZ_AP018042|CRT matches to MN694732 (Marine virus AFVG_250M550, complete genome) position: , mismatch: 7, identity: 0.774

ttgta-attttttaaatctctaatcatatcgg	CRISPR spacer
-gatacattttttatatctgtaatcatattgt	Protospacer
  .** ******** **** *********.* 

5. spacer 7.12|3857521|33|NZ_AP018042|CRT matches to NZ_CP040335 (Bacillus cereus strain DLOU-Changhai plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.788

caattttcaatgctcataattttaattttgagt	CRISPR spacer
ctcttttctatgctcatacttttaatttctagc	Protospacer
*  ***** ********* *********. **.

6. spacer 7.12|3857521|33|NZ_AP018042|CRT matches to NC_004821 (Bacillus prophage phBC6A52, complete genome) position: , mismatch: 7, identity: 0.788

caattttcaatgctcataattttaattttgagt	CRISPR spacer
ctcttttctatgctcatacttttaatttctagc	Protospacer
*  ***** ********* *********. **.

7. spacer 7.9|3857326|33|NZ_AP018042|CRT matches to NZ_CP042840 (Enterococcus sp. DA9 plasmid unnamed4) position: , mismatch: 8, identity: 0.758

acttgtaggttaatactccatcaatgaagaaag	CRISPR spacer
ccgggatgcttaattctgcatcaatgaagaaag	Protospacer
 *  *  * ***** ** ***************

8. spacer 7.16|3857783|31|NZ_AP018042|CRT matches to NC_019777 (Cyanobacterium aponinum PCC 10605 plasmid pCYAN10605.01, complete sequence) position: , mismatch: 8, identity: 0.742

ctctttcccatgatttgaaatttcacattgt	CRISPR spacer
tcttttcccatgatttgtaatttaactcggt	Protospacer
...************** ***** ** . **

9. spacer 7.7|3857194|33|NZ_AP018042|CRT matches to KC821607 (Cellulophaga phage phi19:1, complete genome) position: , mismatch: 9, identity: 0.727

actttgatttttgcataaaagaaataaaatctg	CRISPR spacer
tctttgctctttgcataaaagaaataccactaa	Protospacer
 ***** *.*****************  *.. .

10. spacer 7.10|3857392|31|NZ_AP018042|CRT matches to NZ_AP017383 (Moraxella osloensis strain KMC41 plasmid pMOSL2, complete sequence) position: , mismatch: 9, identity: 0.71

ttgtaattttttaaatctctaatcatatcgg	CRISPR spacer
ttgtcattttttaattctctaatttttgtaa	Protospacer
**** ********* ********. *  ...

11. spacer 7.10|3857392|31|NZ_AP018042|CRT matches to NZ_CP024186 (Moraxella osloensis strain TT16 plasmid p1, complete sequence) position: , mismatch: 9, identity: 0.71

ttgtaattttttaaatctctaatcatatcgg	CRISPR spacer
ttgtcattttttaattctctaatttttgtaa	Protospacer
**** ********* ********. *  ...

12. spacer 7.10|3857392|31|NZ_AP018042|CRT matches to NZ_CP024177 (Moraxella osloensis strain YHS plasmid pYHS1, complete sequence) position: , mismatch: 9, identity: 0.71

ttgtaattttttaaatctctaatcatatcgg	CRISPR spacer
ttgtcattttttaattctctaatttttgtaa	Protospacer
**** ********* ********. *  ...

13. spacer 7.12|3857521|33|NZ_AP018042|CRT matches to NZ_LR214939 (Mycoplasma salivarium strain NCTC10113 plasmid 2) position: , mismatch: 9, identity: 0.727

caattttcaatgctcataattttaattttgagt	CRISPR spacer
tcaatttcaatgctcatatttttaactttttca	Protospacer
. * ************** ******.***    

14. spacer 7.12|3857521|33|NZ_AP018042|CRT matches to KC465899 (Pelagibacter phage HTVC008M, complete genome) position: , mismatch: 9, identity: 0.727

caattttcaatgctcataattttaattttgagt	CRISPR spacer
actgcttcaatgcccataattttaagtttaggt	Protospacer
    .********.*********** ***..**

15. spacer 7.16|3857783|31|NZ_AP018042|CRT matches to NC_027643 (Microviridae Fen685_11, complete genome) position: , mismatch: 9, identity: 0.71

ctctttcccatgatttgaaatttcacattgt	CRISPR spacer
atctttctcatgattttaaatttcgtttaac	Protospacer
 ******.******** *******.. * ..

16. spacer 7.5|3857062|33|NZ_AP018042|CRT matches to MN693292 (Marine virus AFVG_25M2, complete genome) position: , mismatch: 10, identity: 0.697

acatgaaataagttattgtcacttgaattccag	CRISPR spacer
cttttaaataagttatggtcacttgcattgtca	Protospacer
 . * *********** ******** *** . .

17. spacer 7.12|3857521|33|NZ_AP018042|CRT matches to MT682709 (Escherichia phage vB_EcoM_SP1, complete genome) position: , mismatch: 10, identity: 0.697

caattttcaatgctcataattttaattttgagt	CRISPR spacer
acattttcaatgcttataatttcaatatcagcc	Protospacer
  ************.*******.*** *... .

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site
DBSCAN-SWA_1	302839 : 308670	7	Escherichia_phage(33.33%)	NA	NA
DBSCAN-SWA_2	2309575 : 2347311	36	Synechococcus_phage(20.0%)	bacteriocin,tRNA,transposase	NA
DBSCAN-SWA_3	3953764 : 3962878	8	Bacillus_phage(25.0%)	NA	NA

Sequences of DBSCAN-SWA_1

Nucleotide sequences of DBSCAN-SWA_1

>NZ_AP018042|302839:308670|DBSCAN-SWA
ATTAATCATTCAGAACAGCAGCTCTAAATTCCTTAATTCTCTGTTCTTCCTCCTTTTTGCAGATCAACAAAACATCATTGTCTTCAACCACAATACAATCGTTCAAACCTTGAATCACTACTTTTTTATTCTTTGGCATGTTCACAATACAATTGCTTGATTCAAAAACATGAACTTTTTCGCCAACTACAGTATTTCCCTGTTCACAACGTTCGCGCTTTTCCCAAAGCGATCCCCAGGTACCCAAATCAGACCATCCAAAATCAGCTGGCTGAACATAGATGTTATCCGCCTTTTCCATGATGCCATAATCAATGGAGATCTTCATGCATGTTGGAAACATTTCGTCGATAAAGGCTTGCTCTTTGTTTGTATTGTAAACAGCTGCCCCCTTTTGAAATAGCTCAGCCACTTCAGGCAAGTCCTTTTCGAAAGCTTTTACAATGCTCTTTAGCGACCACAAGAAAATACCAGAGTTCCAATAATAGTTTCCTGCAGCTAAATATTCTTTCGCTGTAACAAGATCAGGTTTTTCTTTAAAAGCGATTACGTTTAACGGATTGTCTGTTATCGCTTGTCTGTTATCGGTTTCAAAATTCTTACTTATTAATTTTTCATTATTAATTGAATCGGCTTGAATGTATCCATAACCTGTTTCGGGACGGTGAGGCTGAATGCCCAAAGTTAGTAGAGTGTCATTGTTCACTGTAAATTCCAAACCATTTTTGATGACACGAATAAACTCCGCCTCATTGGTAATCAAATGATCCGACGGAGCTACCACCATATTGGCATCAGGATTCAATCCTTTTATTTTGTAAGCTGCATAAGCGATACAGGGTGCTGTGTTTCGCATGCATGGCTCCAATAATATGTTGCTCTCCAACAATTCAGGACATTGTTCTGTTACCAATGCTTTGTACTTGGCTGAAGTCACAATCAGAATGTTCTCAGCCGGAGCAATTGTTTTGAAACGAGCTACGGTTTGCTGCAACAGCGTTTTTCCAATTCCCAAAACATCTAAAAACTGCTTTGGCTTTTCTTCTGTACTCATCGGCCAAAAACGGGAACCGACTCCCCCGGCCATGATAACTATGTAGTTGTTGTTCATTTATTTCTAGAATTAGGCGTTAGCAATAGCGCTATTTTACGAATGGTTAAACGGACTAAGGGTCGAAGAATCAAAGAGTCAAACAGTCGAATACTATTTTTTGCATAGATACTTCATCAAATTGCTTATTGCACCTGATAAATTACTGGAACGTACTCTTAGTTCCTGGGCTTTTTCTATAGTAAGGTAATTAAGGTCTTCAGCCAAATAAAGCATGCTACGCACCTCACCTGATGAGGCTTTTGCAATATCCAGAAATCTTTTGAATTCAGCATCTGAGTTCCGTTCAAAACCTTCTGCAATATTGTTCATGACAGATACTGAAGCTCTTTGAATTTGATCCCGAAAACCAAAATCTCTGTTTTCTTTCAATATTAAATAAACGTCACCAACAAGTACTCTGGCATCCTGCCAAATTTTTAAATCTTCAAACTTCTCGACATTTGCCATGCTAACGACTTTTTGACTGTTCGACTCTTGGACTTTTATACTTTACGACTTATACATCTCCGCGTAGTATTTCTCGTATTCTCCACTGGTGATGTTCTCCATCCATTCCTTATTCTCCAGATACCACTTTACGGTTTCCTCAATTCCCTCTTCGAATTGCAAAGATGGCTCCCAACCTAATTCTTCTTTCAATTTGGTCGAATCAATTGCGTAACGCAAATCATGACCGGCACGATCGGTAACATAGGTAATCAGTTTTTCAGATTCGCCTTCTTCACGACCCAATAATCTGTCGACGGTTTTAATCATCACTTTGATCAGATCAATGTTCGTCCATTCGTTGAATCCACCAATGTTATAGGTATCTCCTACTTTTCCTTCATGGAAAATCACATCAATGGCTCTGGCGTGATCTTCCACATACAACCAATCTCTAACATTTAGACCCTTTCCATAAACCGGAAGTGCTTTGTTGTTACAGATGTTGTTGATGAACAAAGGAATCAATTTTTCAGGAAACTGATAAGAACCATAGTTGTTCGAACAGTTGGAAATTACGGTTGGCAATCCAAAGGTATCCTGAAATGATCTCACGAAATGATCCGAAGAAGCTTTGGATGCTGAATAAGGTGAGTGAGGATCGTAAGCTGTTGTTTCCAAAAAGAATCCTTCTTCCCCTAAAGAGCCGTACACTTCATCCGTAGATACATGGTAGAACAATTTCCCTTCGTAATTTCCTTCCCAGGCAAACTTTGCTGCCTGCAATAAACTCAATGTTCCCATTACATTGGTTTGTGCAAAGGAAAATGGATCTTTAATCGAACGATCAACATGAGACTCGGCAGCCAAATGAATCACACTTTCAATTTTATAGTCAAGAAAGATCTTCTGAACCTTTTCGAAATCACAGATATCGCATTTTACGAATTCGTAATTTTCTTTGTCTTCAATGTCTTTTAGGTTGGCCAAATTTCCTGCATAGGTCAAGACATCCATGTTAATAATTTTATAATCAGGATATTTATTGACCAACAAACGAACAAGGTGCGAACCGATAAATCCGGCACCTCCGGTAACTAGGATTGATTTCATATTTTTACAAATTAAAACCACAAATTACACCAATTTCACAAATTATAATATTCTAAAATTTGTATTCATTAGTGTCATTTGCTGTTATTTCATTAATTTCGGTTACAAAATATTTAAACCACTGATTTCACAAATTAAAAAGAAAACATCGCAAACAATACTTACTCCTCTTGAAATCATACTTCTTTTAAACACTCGTAATTCGTGGCTATTCCATTAATTTCTCAATACATATTTTTAAAGAATCTCTCCAATAAGGAACTTCAATTCCAAAAACCGCTTTGATCTTTGCCTTGTTTAGTACTGAATAATGTGGTCGTTGCGCCGGAGTTGGGTAGTCTTTCGTTTCAATTGGATTTACAATACAATCTACATTTCCCATTTCCATGATCGCTTTGGAAAAATCGAACCAGGAAGCAACACCTTCATTCGAGAAATGATATACCTTCCCTTTTTGATCCATTTTATCTTCTTTGGAAAAAATCTCCAGACATACATTAGCTAAATCTCTTGCATAAGTAGGAGTTCCTACCTGATCAAAGATCACACCTAGTTCAGCTCTTTCAGCTCCCAAACGAAGCATGGTTTTCACAAAATTGTTCCCGAACGAAGAATACAACCATGACGTTCGAATTACAATAGATTCAGACGAAGAAGTAAGAACAAACTCCTCCCCTTTTCTTTTTGTCTTTCCGTACACCCCGATTGGTGATACTTCACAATCTTCTGAGTAAGGAAAATGAGCAGTTCCATCAAAAACGTAATCTGTAGAAATGTGAATCAGTTTCAAATTCTGAGCTTCAGCAATAGCCACCAAATTCCCGACAGCAGTTGCATTTACCAATTCAGCAGTTTTAGAATCTTCTTCTGCCTTATCCACAGCCGTATAAGCTGCACAGTTAATGATTCCTTTTATTTGATTTTCGCTTACGAACCGGGCAAGCATTTCTCTATTGCAAATGTCTAATTCCGGTAGATCGGAAAAAACAAATTCCAAATTTGAAAATGATGCACTAAGCTCATTTATTTCTGAACCCAATTGACCATTTGCACCACATATCAATATTCGCATAATTCCATTTTTAAAGTAAAAGGATTTTCAGTCTCACACAATGGCAATAATTGCAGATCTTTTCCCGACAATTGAACTTCTTCTGCTGCAATTTGCCAATCAACAGCAATCTCTTCATCATTCCAGGCAATGCCTGAATCGTGATTAGGAGCATACCAGTTATCAACTTTATAGGCGAAAGTTGCTTTCTCACTCAATACTACAAAGCCATGTGCAAATCCTCTGGGAACAAACAACTGTCGTTTGTTTTCCCCGTTCAATTTCACCGCTACATATTTTTTATACGTTGCTGAATCTTTTCGAAGATCTACCGCTACATCCAATACTTCTCCCTCTATACAACGAACCAATTTGGCTTGATTGAAAGGAGGTTTTTGAAAATGCAAACCTCTCAACACTCCTCTTGATGATTTTGATTCATTGTCCTGAACAAAATCAACTTTCCCTATGTTTTCTTCAAACTCTTTCTGATTGAAAGATTCAAAAAAGTAGCCTCTATCATCCCCAAACACTTTAGGTTCGATGATGACTACTTCGGGTATTTCTGTTTTTATAAAATTCATAGTTTTTTTTTCAACCACTTTTAAATTCAAAATAACAACCACAAATTTCTCAAATTACACAGATTAAATTCATTTGTGCTCATTCGTGTAATTTGTGATTCATTAACTTTTAACTACAAAATGAATCTTTTGTACTGCAAACTTGTTGCTCCAAAATTGATTAATAGCCCTACTCTTTTACCCGTAGCCTTCAAATAATTTAAAACTTGAGCAGTATGAATATCATCCAAAGTTTTTACAGCCTTTAACTCAACAATAACTTCATCAAAACAAAGAAAATCAGCGTAATATTTCTTATCAAGCTGATTTCCTTTATATACAATTCCAAGTTCTACCTCTCTTTCAAAATCAATCTCTTTTTCTACTAATTCTATTTCCAGAGCTTCTTGATAAACCGCTTCCAAAAAACCACATCCGAGTTCTGAATGAACTTCCATACAACTACCAATAATTTTGTAGCATTCTTTCTTCAGAATATAGTCTTGCTTCATTTTGTAAAAAAGTAAATTTCGAACCACAAATTTCTCCAATTTTCACAAATGAAAAAAGCTTTATTTAAATAAGCAAAATGATCAATCCTCCATCATAAGGGAGGCAAGACTCCATTTTGACACTTAAAAATTTGTGACAATCAGTGAAATTTGTGGTTAATATTATTTGAGGTTTTATTTGTCCGCAACAAGATTTAAAAGGTACTGTCCATACCCATTCTTTTTCAATGGCTCGGCCAATGTTTTAACTTGTTCGGCAGTTATGTAACCCATATTGTAGGCTATTTCCTCCAGACAAGCCACTTTTAAGCCCTGGCGGGTTTCGATGGTTTCGATAAAACGACCTGCATCTAGTAAAGATTCATGTGTTCCTGTATCCAACCAGGCATACCCCCTACCCATTAATTCTACTTTTAGTCGACCTTGCTTTAAATATTCCTGATTGACAGTGGTAATTTCCAGCTCGCCACGATGAGAGGGTTTCACATTTTTTGCGACTTGCACCACATCATTGGAATAAAAATACAAACCAATAACAGCATAATTCGATGCAGGATTCTCTGGTTTCTCCTCTATGGAAGTTACTGTTCCATCTTCATCAAAAGCAGCAACGCCATAACGCTCAGGATCTCTTACATAGTATCCAAAAACAGTAGCTTTGCCTTCTTCCTCTACATTTTTACGCGCATTTCTCAACATGCCCGTAAGGTTGTGACCGTAGAAAATATTATCTCCCAATACCAAACAAACCGAATCATCGCCAATAAATTCGTCCCCAATGATAAAAGCCTGAGCCAATCCATCAGGACTTGGTTGTTCTTTGTAAGATAATTTTAATCCCAAATCTTCACCAGATCCTAATAATTTTTCAAAATTAGGTAGATCCTCAGGAGTGGAAATGATAAGTATCTCCTGAATCCCTGCCAGCATTAAAACTGATAATGGATAATAAATCATCGGTTTGTCGTAAACAGGCAAAAGCTGTTTCGAAACTCCTTTGGTTATTGGATACAAACGGGTTCCGCTGCCTCCTGCTAAAATGATTCCTTTCAT

Protein sequences of DBSCAN-SWA_1

>NZ_AP018042|302839:308670|302839_303949_-|WP_096427554.1|DBSCAN-SWA
MNNNYIVIMAGGVGSRFWPMSTEEKPKQFLDVLGIGKTLLQQTVARFKTIAPAENILIVTSAKYKALVTEQCPELLESNILLEPCMRNTAPCIAYAAYKIKGLNPDANMVVAPSDHLITNEAEFIRVIKNGLEFTVNNDTLLTLGIQPHRPETGYGYIQADSINNEKLISKNFETDNRQAITDNPLNVIAFKEKPDLVTAKEYLAAGNYYWNSGIFLWSLKSIVKAFEKDLPEVAELFQKGAAVYNTNKEQAFIDEMFPTCMKISIDYGIMEKADNIYVQPADFGWSDLGTWGSLWEKRERCEQGNTVVGEKVHVFESSNCIVNMPKNKKVVIQGLNDCIVVEDNDVLLICKKEEEQRIKEFRAAVLND
>NZ_AP018042|302839:308670|307239_307617_-|WP_096433312.1|DBSCAN-SWA
MKQDYILKKECYKIIGSCMEVHSELGCGFLEAVYQEALEIELVEKEIDFEREVELGIVYKGNQLDKKYYADFLCFDEVIVELKAVKTLDDIHTAQVLNYLKATGKRVGLLINFGATSLQYKRFIL
>NZ_AP018042|302839:308670|304441_305491_-|WP_096427556.1|DBSCAN-SWA
MKSILVTGGAGFIGSHLVRLLVNKYPDYKIINMDVLTYAGNLANLKDIEDKENYEFVKCDICDFEKVQKIFLDYKIESVIHLAAESHVDRSIKDPFSFAQTNVMGTLSLLQAAKFAWEGNYEGKLFYHVSTDEVYGSLGEEGFFLETTAYDPHSPYSASKASSDHFVRSFQDTFGLPTVISNCSNNYGSYQFPEKLIPLFINNICNNKALPVYGKGLNVRDWLYVEDHARAIDVIFHEGKVGDTYNIGGFNEWTNIDLIKVMIKTVDRLLGREEGESEKLITYVTDRAGHDLRYAIDSTKLKEELGWEPSLQFEEGIEETVKWYLENKEWMENITSGEYEKYYAEMYKS
>NZ_AP018042|302839:308670|305699_306563_-|WP_096427557.1|DBSCAN-SWA
MRILICGANGQLGSEINELSASFSNLEFVFSDLPELDICNREMLARFVSENQIKGIINCAAYTAVDKAEEDSKTAELVNATAVGNLVAIAEAQNLKLIHISTDYVFDGTAHFPYSEDCEVSPIGVYGKTKRKGEEFVLTSSSESIVIRTSWLYSSFGNNFVKTMLRLGAERAELGVIFDQVGTPTYARDLANVCLEIFSKEDKMDQKGKVYHFSNEGVASWFDFSKAIMEMGNVDCIVNPIETKDYPTPAQRPHYSVLNKAKIKAVFGIEVPYWRDSLKICIEKLME
>NZ_AP018042|302839:308670|307791_308670_-|WP_096427558.1|DBSCAN-SWA
MKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIQEILIISTPEDLPNFEKLLGSGEDLGLKLSYKEQPSPDGLAQAFIIGDEFIGDDSVCLVLGDNIFYGHNLTGMLRNARKNVEEEGKATVFGYYVRDPERYGVAAFDEDGTVTSIEEKPENPASNYAVIGLYFYSNDVVQVAKNVKPSHRGELEITTVNQEYLKQGRLKVELMGRGYAWLDTGTHESLLDAGRFIETIETRQGLKVACLEEIAYNMGYITAEQVKTLAEPLKKNGYGQYLLNLVADK
>NZ_AP018042|302839:308670|306550_307126_-|WP_096433310.1|DBSCAN-SWA
MNFIKTEIPEVVIIEPKVFGDDRGYFFESFNQKEFEENIGKVDFVQDNESKSSRGVLRGLHFQKPPFNQAKLVRCIEGEVLDVAVDLRKDSATYKKYVAVKLNGENKRQLFVPRGFAHGFVVLSEKATFAYKVDNWYAPNHDSGIAWNDEEIAVDWQIAAEEVQLSGKDLQLLPLCETENPFTLKMELCEY
>NZ_AP018042|302839:308670|304042_304399_-|WP_096427555.1|DBSCAN-SWA
MANVEKFEDLKIWQDARVLVGDVYLILKENRDFGFRDQIQRASVSVMNNIAEGFERNSDAEFKRFLDIAKASSGEVRSMLYLAEDLNYLTIEKAQELRVRSSNLSGAISNLMKYLCKK

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096427554.1|302839_303949_-	mannose-1-phosphate guanylyltransferase	A0A1V0SH58	Hokovirus	2.1e-33	30.2
WP_096427555.1|304042_304399_-	four helix bundle protein	NA	NA	NA	NA
WP_096427556.1|304441_305491_-	dTDP-glucose 4,6-dehydratase	A0A291LAD7	Escherichia_phage	5.7e-81	47.9
WP_096427557.1|305699_306563_-	dTDP-4-dehydrorhamnose reductase	K7QJU0	Escherichia_phage	2.6e-39	37.1
WP_096433310.1|306550_307126_-	dTDP-4-dehydrorhamnose 3,5-epimerase	I7HJC4	Enterobacteria_phage	1.7e-42	55.0
WP_096433312.1|307239_307617_-	GxxExxY protein	E5EQF3	Micromonas_sp._RCC1109_virus	4.2e-10	38.3
WP_096427558.1|307791_308670_-	glucose-1-phosphate thymidylyltransferase RfbA	I7I009	Enterobacteria_phage	2.5e-106	63.9

Sequences of DBSCAN-SWA_2

Nucleotide sequences of DBSCAN-SWA_2

>NZ_AP018042|2309575:2347311|DBSCAN-SWA
TCTATTCTTTGTTTTTAGTATCCATTACAATGGTAACCGGACCATCGTTTACCAATGAGACTTGCATTTCGGCACCGAATTCTCCTGTTTCAACTTTCTTTCCGGATTCTTTCTCCAGACAAGCCACAAAATTCTCATACAAGGGAACCGAAATATCCGGTTTTGCAGCCAAAATATAAGAGGGTCTATTCCCCTTTTTAGTTTTAGCATGCAAGGTAAACTGACTTATTGCCAGAATGTTTCCATCAACATCCATCAATGATTTATTCATTACGCCCTCTTCATCATCGAAAATTCGAAGATTGCATATTTTTTTACTTAACCATTCCGAGTCCTCTTCAGTATCGGCAGTCTCAATCCCTACCAAAACCATAAGTCCTTTCGAAATTTGTCCTATTATTTTAGAATCTACACTCACCGTAGCTTGCGTAACCCGTTGAATCACAATACGCATATCAATATGTTTATTAAGATTGTAATTTATTAGCCTTTTCAAATATAACACAACAAAAGTAAGGGCAATGAAATAGCTAAAGCTGATTTTCCTTACAAATTCACTATTAAAATTAAGTCTAAATTAAAATATTCCCCAATAAATAATCATATTTGTAGGATCGAAAAATTAACAAACAAAACATGCTGTATTTTATCAGTTCATCTACTAATCCGTCCTTTAACATTGCCACCGAAGAGTATTTGCTAAAAAACTACGAGGACGATTTCTTCTTTTTATACACCAACACTCCTGCCCTAATTGTTGGGAAACATCAGAATACTTTGGCTGAGATAAATTTGGAAAAAGCCGAACAGGAAAACATTCCGGTTGTAAGAAGAATGTCTGGAGGAGGTACTGTTTTTCACGATGAAGGAAATCTAAATTATTGCTTTATTAAGAATGGGGAAAAAGGCAAATTGGTTGACTTTCAGAAATACAGTCAGCCGATTGTTGATACTCTTCAGGCGCTAAACGTAGATGCAAAGTTCGAAGGAAAAAGTGATTTAACCATTGATGGAAAGAAGTTTTCAGGCAATGCTTCTCACATCTACAAAAACAAGGTCATGCAGCATGGCACAATGCTGTTTTCGAGCGATTTAAAGCAACTAAACCAACTACTAAAGGTTAATCCTCTCAAGTTTAAGGATCGAGGCATTAGATCCATTAGAAGTAAGGTTACCAACATCTCAGAATACCTTTCCGATTCAATGAGTTTAGAGAAGTTTGGTCAATTAATCTTAGAAGATTTAAAAACGAAGTTCCTGGATGCCAAAAAATTTGAACTATCAGCAATTGATATTGAGAAGATAGAGGCGTTAATGGCAGAAAAATACAATAACTGGGAATGGAACTATGGCTACTCTCCCAATTACATTTTTGAAAAACTTAGTAATTTCCCTGGAGGTGGCATTCTAGAACTTAGCATGAAGGTAGAGAAAGGAATCATTAAAAATCTTGAATTATTTGGCAATTGTCTTGTAGAAAATCAGATTTCCCATTTTAAAACTAAATTAATTGGTAAATATCATGATAAAACCACTATTAGAAAGACACTTAAAGAACTTGACTTAAATCTATTCTTTCATGAAATTACTGATAAAGAACTCCTATCAGCTCTTTTTTAAGAATCATATTTCAGTAAAATTGAAATTACAGCACTATTAAAATTTCTACGAACACCTCAATCAATAACATCTAATTAAAATATTTATGGACTGTTAAAGCTTTTGCATTACCCAGTTCAGTTTCCAGTTCAGCAACAGAGGCCTTCCTCACATTTTCAACAGACTTGAACTTTTGAATTAATTTTTGAACTGATTTTGGTCCGATTCCCTCAATTTTATCCAATTCAGAAACAATAAATGCCTTTGATCGCTTCTGCCTATGAAAGGTAATTGCAAAGCGATGCGATTCATTTCGCAAGTGCTGAATGGTTTTAAGAGTCTCTGAATTCTTATCCAGATACAAGGGGAAAGGATCACCCGGAAAATAAATTTCTTCCAATTTTTTAGCGATTCCGATTACTGAAATCTTTCCACGCAATTCCAATTTATCCAAACTATTCAAAGCTGCACCCAACTGTCCTTTGCCTCCATCAATAACAATTAGCTGTGGTAAACTTTTGTTTTCATCAAGCAATCTTCTGTAACGTCTATAAATTATTTCTTCCATAGAAGCAAAGTCATTTGCTCCTTCTACCGTTTTTATATTAAAATGACGATAGTCTTTTTTATAAGGTCTTGCATTCCTAAATACAACACAGGAAGCAACGGGAATAGTTCCTTGTATGTTCGAGTTATCGAAACATTCAATATGAACCGGCGCATCTTTCAATCGCAAATCGGTTCTCATTGTTTCAACAATTCGATCGGCATGAGATTGTTTTATTGACTTATCCCCCTTCTTTAACTTCTCCAGACGGTAATATTTGACGTTACGTTCTGATAAATCCAGCAATTTCTTTTTGTCTCCCCGCTGAGGAACAATAAACCGAACATTTTCTATCACCAAATCAAGTTCGATAGGTACCAGTATTTCCTTTGCTGTACTGAAAATCTTTTGTCGAATGTCGGTAATAGCCAATAAAAGTAAATCCTCTTTACTCTCATCCAGCTTTTTCTTCATCTCAATGGTATGGGCTTGAATAATTGCCCCATTAACAACCTTTATGAAATTTACGTAAGCAAAGTTCTCATCATCGACAATAGAATAAACATCAACATTATTAATTGATGGATTTACAATAGTAGATCTACTTTGATATTTTGATAGTAAATCAAGCTTTTCTTTAATGATATGAGCTTCCTCAAATTTAAAATCAACAGACAATTCCTGCATTTTTTCCTTCAAGTGTGAAGTTACCGTACCAATATTTCCTTTTAGAATATTTCGTATTTCTGCAATTTTCAAATTGTATTCTTCTTGATTTTCCTTCCCTTCACATGGTGCTTTACAATTTCCCATATGAAATTCTAAACAAAGCTTAAACTTTTGATCCCTTACACCCTCCTCACTCAATTTTAAATTACAAGTCCGCAATTGATACAAAGAACTAATCATGTCCATTAGAACTCTCACCATTCTAACACTTGTATATGGACCAAAGTATTCGGCTCCATCTTTTAGCAAGTATCGGGTGACAAGTACTCTTGGAAATTCTTCTTTTTTAATGCAAATCCAAGGAAATGTTTTATCGTCTTTTAAGAGAACGTTGTAACGAGGCTGATATTTTTTAATTAAGTTATTTTCCAATAACAAAGCATCAGCTTCCGACTCCACCACAATGTGTCGGATGTCCACAATCTTGCGAACCATTATATTGGTTTTTGCCGAATCGTGAACTTTATTAAAATAGGAAGCAACTCTTCGTTTGAGTCGCTTAGCTTTTCCTACATATATAATAGTATTGCTCTCGTCAAAGAACTGATACACTCCCGGCTCTTCCGGCAATGCCGAAACCAAGGATTTTAAATGATCTGTATTTTTGTTTTTTATCACCTATTCTAATTTTCCTCTAATATAGCTTACTCTTCTTTAAAAAGATTAATCACCTTAAATTCTTTTACATCAAACAGACCTTTATCCGATAATTTAAGTTTTGGAATAACGAGTAAAGACATAAATGCAAGTGTCATAAATGGTGATTCAAAATTTGAACCAAGAGCCGAAGCAAAATCATTTAATTTGGCATACTCCCTAACTAAGTCTTCCCCGGTCTTGTTCGACATCAAACCAGCAACCTCTAATCGAACCGATTCACTTTCCCCATTATTTACAGCAACAATCCCACCCTTCATTTCAATTAAGGTATTAATTGCATGTAACAATTCTTCATCGCTCGTGCCAACAGCAATAATATTGTGACTATCATGAGCAATACTCTCTGCAATGGCTCCTTTTTTATAACCAAAGCCTTTTGCAAAACCCATTACCGGATTACCATTATCGTATCGACTCATCACAACCATTTTCAACAAATCATTATCTACATCCGAAACAACATTTTTATCCACAACCTTCGGTTTGCACAAAACGGAATCCGTTATTAGATCACCATCCTTCACCACCATTGCACGCAATGAACCATTTTCCGCTTTTACAATTAAATCGGATAACTGAATTGGTTTGCGACTAAAATTATTCAAGATAATTTCATTGCTTACATCAAACAAAACCTCATTGTCTTTATAAATGCAAACTCCTTTTCTAAATGCTTGCTCTACAATAAATGATTCAGGATTATCAATCACAATAAAATCTGCCGAATGACCAACTTGAAGCAAACCCAAATCTAAACCGTAGTGATGAATTGGATTGTAACTTGCTGCCCGAAGTAATGAAAATAAATCAAGTCCTTTTTTCAGTCCTTTTTTAATCAGGTAATTTATATGCCCTTTTTCAATTAAATCATCCGGGTGTAGATCGTCAGTACACAGCATGATTTTTTCTGGATGAGTTTCTAATAAAGAACAAAGTGCATCAAAATCTTTAGCGGCGCTTCCCTCTCGTATTTGAATCATCATCCCTAATGAAATTTTTTCAAGAGCTTCCTCTAAAGTAGAACATTCATGATCTGCAGAAATACCTGATGCCACATATTTTTTCAGATCCTGCCCTTTTAATCCAGGAGCATGACCATCTATTTTTTTCCCAAGTTTTTTACAGGCAACTATTTTTTTGAGTACCTCAAGATCATCGTTTATTACCCCTACAAAATCCATAACCTCTGCAAGACAAACCACCTCTTCTCTCTTCAGTAAACTTTCCACAATATTACTGTCCAAAACAAAACCTGAAGTTTCGTGAGGAGTTGCAGGAACACACGAAGGAACACCGAAGCGAATTTCCAACGGAACTTTTTTAGCATCATTAATCATGAAATCGACACCAATCTCGCCCAACACATTTGCTATTTCGTGTGGGTCTGTCACCAATCCCAATGTACCCTGTCGAACTGCTAAATTTGCAAATCGACTTGGTATCAAGAGCGAACTTTCAATGTGCATATGTGCATCAACAAGTCCTGGTAAAATATAATGATCCGGAACAGAATCTCTTTGCGTAACGGAAGTAATTCGCCCCTCTTCAACCACAACTTCAGCTTTGATTATTTCTCGTGAAATTACATCTATCACTTTACCTGATATTGAAAATGTATTACTTGTCATGTCTTGTATATTATCAATTTTAATGATCTTATTAAATTCATAGAATAGCTCAATATTGCCTTCAAAAATGCCCGAAATAAGCCTTCTCAAGTGTGAAAAGCCGATTTCGGGCATTTTAAGGAGTAAACTATTTTAAAATACTCCTTAGTTCCACGTGGAACGTTATCGTCCCATGAGCACTAAAAGAACGTTTATGTCGCTAGGTGAAACACCCGATATACGAGATGCCTGACCGATGGTTTGCGGTTGTATTTTATCCAATTTTTGTCTGGCTTCAGTCGAAATTGATTGTAAACTTGTGTATTCAAAACGACCTTTTATATTTATATTTTCCAAGCGTGTTAGTTTTTCTGCTATCAACTTTTCTCTGTTGATATAGCCTGAATATTTTATAAGAACCTCCGCCGCTTCAATAACTTCTTCTCGCCTGCTTGGAATTGAATCTATCAATTCTTTTAACGGTAATACATCTTCCACTAAATCCAAAATTTGAACTTGAGGTCTTAGAATAATATCATATAATTTCACCCCTTGCTTTAATGGAGCTGTTCCTAATTTCGCGAGTACTGGGTTTAAAAATTTTGGTTTGCAACTAAACTCTTTACAAAAGGTAACCAATTTATCTCTAAACTCAATCTTCTCTTTTAGCAAATTCATTCGTTTTTCTCCAACCAATCCCAACTCAAAAGCCTTCGGACTTAGTCGTAAATCGGCATCATCCTGTCTTAATAAAATACGATATTCTGCTCGAGAGGTAAACATTCGGTAAGGCTCATCAACACCTTTTGTTACCAAATCGTCAATTAGAACTCCAATGTAAGCTTCATCTCTATTTAGTGTAAAAGCGTCCTTTTCTTGTACCGACAAGCCTGCATTTATACCTGCCATCATTCCTTGAGCTGCAGCTTCCTCATAACCGGTTGTTCCATTAATCTGGCCGGCAAAAAATAAATTAGAAACTAATTTGGTTTCCAGTGTATGATAAAGTTGAGTGGGATCGAAATAATCATATTCAATTGCATATCCCGGACGATACATTTTCACCTTTTCCAAACCCGGAACTTGCTGAAGCGCTTTTAACTGAACATCCCAAGGTAGACTGGATGAAAATCCATTGACATAATACTCATGAGTGTACTCTCCTTCAGGCTCCAAAAAAAGCAAATGCTCTTGTTTCTCTGCAAAAGTTGACAGTTTAGACTCTATACTCGGACAATATCTTGGTCCTGTACTCTGAATCGTTCCATTGTACATTGGTGATTCCTCAAATCCTTTGCGTAATTCATCATGAACTTTTGGATTTGTATACGTAATATAACAGGGTCTCTGAGTAAGAGTTCTATCAACATGGTCGGTTAAATAAGAAAACTGATAAAATCCTTCTTCCCCATCTTGCCTCTTCATTAGAGAAAAATCGATACTTCGACCATCTAAACGGGCCGGCGTTCCTGTTTTCATTCTGCCTACCTCAAAACCGACACTTTTCAATTGTTCACTAATACCTACTGAGGCTGGTTCTGAAGTTCGCCCCCCTTTTTCTTGCTTGCTGCCAACATGTATTAATCCATTAAGAAAGGTTCCATTAGTAAGAATTACTGATTTGGCTGGAATATCAATTCCTAATTTGGTTGTCACCCCAACAGCTTTCCCCTCTTCAATAAGTATCCCAACAACGGCATCCTGCCACATGTCAAGATTATCTGTATTTTCAACAATTTTTCTCCATTCTGAAGAAAAAATCATTCTATCGCATTGTGATCTTGGGCTCCACATAGCAGGCCCTTTGGAGCGATTTAGCATTCGGAATTGAATGGACGAACGGTCGGCAACTATTCCTGTAAACCCTCCCAAGGCATCTAATTCACGAACAATTTGCCCTTTTGCAATTCCTCCAATAGCTGGATTACATGACATTTGAGCAATTTTATTCATATCCATGGTAATCAAAAGAACCGAAGATCCCAGATTTGCCGCAGCTACCGCCGCCTCACATCCGGCATGACCGGCACCAACCACTATAACATCGTACTTAGGCAACATATATTATAAGATATTTGTATTCGTTATTCTTTAATATTCTTCCGTGAAAATAATCATTCGCCTACACAGCTGATAACCAGCAACATACAAAGATTAAACCCTGGCTGAAAATCTAGTATTCAGATTAATAAACTCAAAAAATTTTACAAATTTAATCAATATTGGAAAGTAATGCTAATGATTCGTCTTCCTTTTGAGTCATTATTTCCTGATCCTCATCAGTTTTATCGTCAATTTCCATTAAATGAAGGATTCCATGAATCATTACACGATGCAATTCCTGAAAGTAATCGCATCCATATTCTTTTGCGTTATCCAGAACTGTATCTATACTAATAAATAAATCTCCTGATACAATATCAGAAACGGTATAATCAAAAGTAATAATATCCGTAAAATAATCGTGATTCAGGTGTTCACGATTCATTTCAAGCAAGTAATTGTCGGAGCAAAAATAGTAATTTATCTCCCCTATTTCGTAACCATTAGTAACAACAACGCGATTGATCCATTCGCTTAAACGCTCCTGATCAATCGCGGGAATATTAGTTTCACACGAATCGTAAGTAATCACCATTTTTATCCAAAAATAAATTTCATTTCTACTATTGCACCATTTTTAGCGAATTCAATATCATCCGCTAAATGATTCATTAAAAAGATTCCTCTTCCATGTGTTTTTTCCAAATTCTCAGGTAGAGTTGGATCAGGTATATTCGAAAAGTCAAATCCTTTTCCTTCATCCCCCACAATAAAGCTTATCGACTCATCATCGATATTGTATTCAATTTTCACCTTTTTATTAATATCTAACTGGTTGCCATGCAAGATTGCATTATTCACTGCCTCGATAATGGCAACCGAAATTTTCCCGTAGATTTCAGAACTAAGGTTATGAGATGATGAAATTTCATCTATGAGTTTTTCAACTCTACTTATATTTTCTAATTCGGACGGAAAGACTAGCAATTTTTCTGATTTAGTCATTATTAATTTTGATCATGTAATCCAAATACAATTTATTATAGTAATCGAGCATCTTTACATAAGAGTCATCAAAAAGTCCCTCAAAATCTGTTCGTACTCTTTTATACTCAAATATTTCTATAGGGTTACTCAATTTGATAATATTTCCTGAATTACTTTCCCTTTTTTTGTCAAAATCTTTTTCTCTATGGGCGTTTTCAGCTTCTAATAAACGTGAAATTATTCTATTCTGTCTATTAACGGTATTCGAATTTATAGAAAAATTTGATAGATCCAATTCCGTTTGATCAATCATTTTCATTATTTCACGTAATATATTCTCTGTTTCATCTCCTACACCTCCTTTTTGCATCATTTCGCTTAAACTTTGTTTAAACATTTCCTGCTTTTGTAAGAATTTGCCGAGCTTCTCTGATGAACTTTTTTTGCCATTTCCTGATTTCATTTCATTAATCATCTCCTCGAGCATCTTCTTTAATGACTGCTGCTCTGATCGCATACCTGAGAAGGATGGCTTGCCTCCTTTTTTATCGCCTATTTGATCTCCAGGCATGGCATTTGCCATTTGCTCCATTAGCTGTTTCAATGCCTCGGAAAGAAACAATGACAATTCATTAACTTCAGTTAAGACTTTTTGTTGTGTAATGTTGGCTTGAATTTTTCTACGGTTGTTCGCTTCTTCAAGAACCTGATCGAGATAATTACTAATATCAAAAATTCTATTTCCAATTAATGCCGCAACCTGAGGTGAGTGAGCTGAAAGAGAAAGTAAGGAATCCTGTATTAAAAGATACTCTTCCTTTAGTTCTCCCTGTTCCTCAATCACTTTAACAAATAGCGGATTTCGATAATCTACCGTTTTGTAATCAGTTAGTAAGGCTTCCTGTTGAAATGAAAATTCCATCAAGTTATTCAGTAAGCGAATCAAATTATCAATATCAACGGTAAGTTGAGCACCTACACTTTGAGACAAATTATTATTCAATTTTTGGGCAAGTGCTTTTTGTTTCTTCGAAGTGGCTTTCATATTTTTTGAAGCCTTCGCATTTTTTCCTTCTTCTAATAAATTTTCGGATTCTTCCATCATCTCAGAAATCTCCTGAAGATCTTCCGATGGATTTTGAAATTGATACGGATCACTAATGCCCGAATTTTTCTCAAGAAGATTTTCAAGATCTTTTTCTAAATCCTTCCATTTTTGCGTGTCCTTAAGCTGCTGAGAATTCATTTTTTCATCCTGCTTGCGCCCTAGGGTACTAATATCCTCTTGATTGTCGGCTATTTTATCGATCCTTTCAATTATTTTACTCATACGAGTCTCAATATCATAACGTTCCAGCAAACTCAGGTTGCGATCCATTTGCTTCTGGAAATCTTCGAACGACATGTTTAATTGGTTCCCAAGCTTGTTTAAATCATCAGATTTAAAATCTTCGGCTAATTTGTTAAATTCTTCAAATAATTTCTGCAATTCATCATCCATTACATTCTCAAGAAGCTTTTCAATTTGTTTTTGCTTTTCGAGTAGCAAAGCATCTTTCTCTCCTAAAGAATTCATCAATTGATTTTTCTTCAGATTTTCATTTTTGATGGTATTCAGCAATTCATCTAATTCTTTCTTTTTAGATTCTATTTGTTGAAACATTTGTTGTTGCTGCCATTTGTCTCCGGTTCCATCCAAAAAGTTTTTTTGAAGACGTTTTATATCTTCTTGAATGGATTGACTCAAATCAATTCCTTTTTCAATTCGATTAGAAATACTATCCTGAATGTTTGTATTTAAATCGTATAATTGTTTCGAATCTGGTATTAATAATGAAAAATGTTCGGAACGAGTCATCTTCGGTTTATTTACCTCATCGTTATCAAAAACTTCAAAATAATATTGAACCTGAATTCCTTCACTTATATCTACTGTTGCAAAATCGAAAGCAAAATAAAATTCTTGCGAACTGAGTTGACGACTAATCGTAATTGGAATGTAAATTGGTAACTGATCATTAATAAGGTAACAAAAACGCAATTTTGAAAAGCCATAATCATCCTTCAAAATCCCTTTGAAATAGTATCCAGTAGTCAATACTGAATCTTGTATGCTTGAAACTTGAATTTGCGGATAAAGATCAGGAATAACATCAACTGTGTAATTGGCAAAAAGCTGTTTATCAAAATGCTGATTGCTTAGGAGAATTTTGTAGGCATTCTTTTTGAAAAATTTTTTGGAAAAAGAGAATGCATCACCATCCTTTTTTGTATCCAAATGATTGAGTGTATAACTAAAAATAAGTTGAACTTCATTGGTGTCTTCACCTTCAATTGTCCATTGCAGAGATGCTCCTTCGGGAACACTAATATCACCCAAATTTTGCTCCTTTCTTTCTGCTATTTTTGTATAAGACGGAGAGTTAACCGAAAGCTCAAAAGACTTAATCCCAGGCTTTAGCAAGAGCTCCAATTGATAGGTTAACGAAACAACCTTTCCTGCTTTTAAATAGAAAGATACATCGTTATTTACATTCCTGAAAAGATAACTAAAGCCATTGTTTCCTTCCTTTTTTAAAAGAAACTCGTTCCCTTCAGAAACCAGGTAGACATCTGAAGGCACATATTTACCTTCTGTATGTACTTTTAATAAAAAATTAGAGCCTCTAACAACTTTCATGTGTTGTTGGTCTAAAACAAATTTAAAGTCGGCTGGTGCAATAAATTCCTTTCTAAAATGGACCAAACGATTCGCACTGCTGGAATACATATCCGGCAAGAAAATATAGGTGCCGATAATTGCTGTGAGAACAATTAGCATGTATTTTAAGTAAGAAATGTTTGATTTAAATGATATTGCTTTTCGAAAAGGAATTAATCGAATAGATTCCATTCTTTGACTAATACTAGCCATTAAAAGAGAATCCTTAGTTGATTCACTTTTAGCCGAAAGCTCTGTAAGTTCAAGCGTATTTACCAATCGATCCTGAAGATCCGGAAAGTATCGAGTAATAATTTCAATTGCTTGCCGGTAGCTGATTCTTTTCCCAATCTGAAATAATCCAATGGTTGGTAAAATAATCAGTTTTATCAGCATTACTGAAAAAACCAAGATGGAAAAGGCAGCTAAAAATGTCCGAACCGGAACTGAAAAATAAAGCAGGTATTCTGTATACGAAACAATCAGAAAATAGGTCAATATCAGAACAATACTTAAAATCGAGCCCCTTAGGAGTTGATTTTGATAGTATTTTCGAATAAATCGATCTAATTTCCCGATAAAAATTTCCTGACTAGAATTCATACCAACTACTACGTAAAAGAGTTAATTACGATACAACCACAAGGAAGGATCTAATTTTGTGCTCCCTTTCCATAGTTGGAATTTAAGAACTGTTTGGCTTTGCGCCTGATCGGTGTAAACCACACCAATTTTTTCTTTGGAAGTAACGGTATCTCCTTTTTTAACTGCTACCGTCGAGAGATTTGAATAGACTGAGAAAAACTCACCATGGCGAATAATTATCACATTGTTTAGTCCTGGCATCGATAGAATATGAGTGACTTCGCCTTTAAAAATAGAGCGACACTCCGCTTTTGTATCGGTGGTAATATTAACACCATCGTTTCGAACATTAACATGCTTAAGTACGGCATGCTTGTGTTGTCCAAACTTCTCTGAAATAAAACCTGTTTTTGTAGGCCAAGGCAATTTGCCTTTATTTTTATCAAATGAATCAGAGAGAATTTTTTCTTCGGGTGTTAAACCGTATTTTCCTCCACCATTATTCTTTTTCGACGCTAACTTTGCCTGATTTTCAATGATTTTCTGAATTTCATTTTCGAGTTTCCTAGTATAACGCTGTTGTTTATTCAAGTCATCTCGAAGTTGTCTCTCTCGCTGTTTCAATTTTGAAACAATCTCAGATTGCTGAGACTTCTCTTGCGCTAAACTCGTATTCTCCTTTGTTTTTTCTGACAATAAAATAACTTTTTCATTCTTGGCAGCTTTTAAATGCAATAAAGTTGCATCCAGAGTATCTTTTTTAACAGCAATCATCTTCCCTTGTTTCCTAGTATATTCAGAGAATTGCTGTAAGTATTTGTAGCGTTTATATGCTTGATTGAAATCCTTTGAGGACAGCAAAAACATCAATTTTTCGTGTGAATTTCTTTGCTTGTATGCATACTGAACTACCTTCGCATATTGTTGCTTTAGTTTCTTCAAATCTTCCTCTAAGCCCTTTACATCAGCATTAGTTTTGCTGATTTGGGATCCCATATATGAAATTTCATTTTCTATGGATTGGATCAAAGCCTGACGGGCCTTTATCCTTTGATTCAGAAGATTTAATTGCCCAAGACTAACATTTTTGTTCTTTAACGTATTAGTTAGAAGAGTATTGGTATAAGCAATATCTTTAGCATTCTGCTCTTTCCTTTTCTTGAGTGTAGAAATATTATTCTGAGACAGGGCATCTACTGAAAATAAAAACAGAAGACCAATCACTAAATAAAATATTTTACCCTTTGTAATTTTGCTTATTCCCATAGTGGTTTTATGGATAAATTCGATCGTATTTATCAGGAATTGTAAACGAAAACCTCAGCTTTTCATCAAATGTTATCTTATTAAATTTAATGTTACAAGATAATAAATGTTTTGGATCCTTAACTTCAAAATTCAATCGTTGAGGAAACTTCCTGTTCTGAAAAACGGTAAAGTCACGATACTTAATTGATATTTCTCTGGAAGTATCGAACTCTTTCACAAAGATATCAGTAATTCTCATTAGTAAGGGCTCGATATCAATTCTTTGATAATTATACTTATCTAGCTTATCCAGCTTATCCTTTTTTAATTTCCGATCAATTTTCCTCGCTTTTTCTGATATGAAAACATACTTATTCCCAATTATTTTCCCATTGAAAGATCTAATAAAAGCCTTCTCTTTCTCCTCATTATCAAGCTTAAAAATTGAATTGGTAAGAATACTCTGAAATGAATCAAAATCCAAATCCAAATTTAGCTTTTCCGAAAAGAAATCAAAACCATCAATAAGATAGGTCTTTTTAAGACGATCAATCATTTTTACCGAATCGGGGGTAATCAGTATGCGAGCTACTGGTATGCCAACTGTGGCCGAAATATCAATCCAAATGATACTGTCTTTTTCTATTTTAATGGAACCTTTAAAACTTTTTGAGATTCCATCTACAGAAAAATTAGCAGCAAATCGCTTTACGTATAAAGTTTGATAATTTAGGCTACTATCTATTAATCCACTATAAAGTTTATTATCCGACATTGTCTTTGCCTTCTCAACCCCTAACTTATAAAAAGATTTACAAGAAAACTGAAAAAGGGCAATAATGATTACCAGAACCCTCAAAGTAGTTTTTAAATATATTCTATTGCTCATTTTGTTCTATCTCATCAGGAATAATGCCTGTTTTAACTTTTTCAGCCAAATACTCCGAGCCTTCTCCTATTTCAATTGCTTTTTTCCACTCTTCAAGAGCCTTGTCAGAATCACCCAATCGAAAAAGAATATCTCCGTAGTGCTCAACAATTACAGCAGATTCTCCGCCACCAAACTCCAGTGCTTTCTCCAATATTGTCTTGGCTTCTTTAAACTCTCCCAATCGATACAATACCCATCCATGAGTATCAAGATATGTTGAATTTTCGGCCTCCAGATCAACACAAAGTGAGCTCATTTTTTTAGCTTTTTCAAGCTCTTCACCTCTCACTGATAAATAGTAACTGTAGTTATTCAATACAATCACATTTTGTGGCTCAAACGACAATACCTCATCATATGATTTGAATGCTTTTTTGGCCTCACCCATTTGATAGTAGGAATCTCCAAGATAAGTTTGAAACTGAACTCGTAACTTTACATTATCACCAATATAGGAAAAACCCTCTTCCAATGTTTGAATTGCTGTTTTATAATCTTCTTTTTGAGCTGCCGCAACACCTTTAAAAATATAAAGAAGTGGCTGATCTGGAAAATATTTTAATGCTTCCGAACTTTCTTTCAACATGCTTTCAAAATCCAGCAATTCATTGTCAATCAAAAGTAATTCTTCCCAAACCACATAATTGGTCTTTTCTTCTTCCAGAACTTTCCTTAAGTGATATCGTGCACCTTCATTGTCTTTTCTCTTTCTAAGAAAATCAGCATAAAGAGCATGCACTCTAACCTGACCTGGGTGAATTTCAACTAATTTATCCAGTAATCCTTTTAAATATTCATCAGACATACTCTCCTGATCTCCATTCATAAGAATCGAAATGAGAAATTGGATTTTTTGATCAGCCTCTATTTCAGAATTTCCGAATGCTTTGGCAAGAGAAACATTACTCTTTTCTATTTCCCCTTTACGTCGGTAGAAATCAGCCAAATAAAAGTGAACCAACCCATTATCCGGATCAATTTTCAATATTTTTTCATACTGGTCAAATGCTTTACCTTCTTCTTTATCGGCCAGGTATAAATCTGCAAGTATCCCATAAAAATCAGCACGATATGGATGTTTTTTTATCAGTTTTTGAATCTCAGCATAAGCCATTTTTTTATTTCCAGCAGCCAGATACAGGCGTTGCTTTTCCAAAGAAACTCCTTCTTGAATCCCTTCTTTTTTCTCCAAACGATCATATACCTCAATCGCTTTGTCGAATTTTTCAACTGAAGCAAAAATAGCCGCCTGCAAATAGTAAAAGTCCTTCCGTTCCGGATGCAATTCTATTAAATCATCATACACAGCACAAGCCTGATCCATCATGCCCTTTTTCTGATAAATATTTGCCAGTTGAATCTGATACCAAATATTTAATGGTTGCAATGCAACGGCTCCTCTTGAAAGTTCCAATGCACTGCTAAAATCGTCCTGACTCATATAGATATTTGCCAATTCGTATCGAACCACAGCACTGGTTGGATCAATTTTAAGACATGTTGCATACAAAGAAAGTCCTTTATCCAACTCTTGAAGCAAAATAGCCTTTGTCGCCTCGGTAAAAGCAAAATCAAACTCTAATTTTTGTGCTTCTGTCAACTTTACTTCTTTCTGTATTTTATCTTTTTTAGGCACTAAAACAGCTCCTTCTGTTATCACAGGAGTTGTTGCCATACAAGCAAAAAGAATTGCTATCCAAAATTTACATCCCACCTTCATCCGTCTTTATTTTGAGAAGGTCGCAAAATCTCCAACATTCCACTCCGAAACTTCACCTTGTATATCAACATAATTACCTATCATGGAGTTTTCAAGATTCAAGTTTTCAAGATTTGAATTTCGCTGAATAATAGAGTTGCGAACCAAAGTATTACTAACTGTCGTATTGGCACCAACCGAAACATGAGGCCCAATTACCGAATTTTTAATTTTCACATTTTCTCCGATGTAACATGGAGATATTATAATGGAATTTTCTGCTGTTGAAGAGGAACAAACCAATTCATCACCTTTGTGGTATTCCAATATTCTTTGATTTGTATGAATAGTTGCATCCTTATTTCCACAATCCATCCATTCAATTACTTGACCCGGTTTAAATGAAAGCCCTTTATTCTTCATGTTCTCCAGGGCATCTGTAAGCTGATATTCACCATTTACAACCACTTCATTATCCATCAGGTATTTCAATTCATCTCTTAGGTTATCACCATCTTTAAAGTAATAAATACCTATAATTGCTAAATCAGAAACAAAGTCTTTTGGCTTTTCAATAAAGTCAGTGATGTTGTTTTTGCCATCAATTTTAACAACTCCAAAGGCCGAGGGATCTTCTACTTTCTGCACCCAAATCACACTATCCGCTTCAGCATCCAAAACAAAATCGGCTTTAAACAATGTATCTGCGAAAGCAACTACTACCTTACCCGATAAAGATGGTGCCGCGCAATAAATTGCATGAGCTGTACCCAATGCTTCTTCCTGATGATAAATGCTCGCTTTAGCCCCAATATTTTCAGCAATACCACGTAATATATCTTCAACATCCTTGCCAAAATCACCAATAATAAAAGCGATTTCATCAATCGCTTCATCCGAAACTTTTGCAATTTCCTCAACAAGACGCTGAACAATAGGTTTTCCTGCAATTGGAATTAAAGGTTTTGGTACGGTTAAAGTGTGAGGACGCATGCGTTTCCCCATTCCTGCCATTGGAACAATTATTTTCATTTGTTATAATATATTGTAGTAGTGAATTGTAAAATAACTAAAAACAAGATCAAATGTACAGTTTTGCAATTTGTTTTGATCCAAATTAATAATTTTTAACGCATTAAGATTTTCCCGTGTGTCCAAATCCTCCTGCGCCTCTTTCTGATGCTTCCAAGTCTTCAACCGGCTCCCAACTTACTGTTTCATGAGCAGCAACAACCATTTGGCAAATTCTATCTCCATCTTTAATCTCTACAAGGGTATTTGAAAGATTAGCAAGAATCACTCCAATTTCTCCACGGTAATCTGCATCAATGGTGCCTGGTGTATTTAATACTGTCACCCCTTCTTTTAGGGCCATGCCCGAACGCGGACGAATTTGAGCTTCGAATCCTGCCGGTAACTCTATAAATAATCCGGTTTTAATTAATTTACGTTCCAAAGATTTTAAAAATACCGGTTCGTTTAGATTTGCTCTAAGATCCATACCTGCAGATAATTCTGTGCTATATTTAGGCAGATCGTGTTTTGATTTATTAACGATTTTCACTTTCATATGTTTATAATTGTATGTTTTTGCCATATGGAACTTACCATGCTGAGATAATCATCTCCTTTATGTCAAAATCGCTTTGGCATGGACGTTTCATATGAATGACAATTGCTTGAAGAATATTACACTTTTTTTCGAGTGGCTAAGATACATTTTTCAAAACTCTTATAAAAGGTTGAGAAAAGGTCTTGATTATTTTCTACTCTTGCTTACTCCAATCAGTTTTCCCAAAGAACCCCACAATCGCTCTTTTTGAATAACCACAACACCAAACAGCAATAGTAATGGGGTTTTAGCGGCCATTTTTAGCCATTGATTTTCGATACCTATATAAGTGCTAGCGATAAAAACGACCAAAGCAAGAAATAAATAAAACAGAATTCTCTTTAAATCGTACTGTACCGGATAATACTTTTGCCCAGCAAGGTAGGATATTACAGTTATTGTGAAAAAACAGGCGAGAACAGAATAAGCAGCTCCCATATATCCAAACTTGGGGACCAGAAGAATATTTAAAACAACGGTGATTATTGTGCCAATAATTGCAATGTAAGCACCAAAACGCGTATTATCCGTTAGTTTGTACCATAAGGATAAAGAGAAGAATATCCCAAAAAATAAATTTGCCAGAAGTACATATGGAACAATTTTAAGCCCCTCATGGTAACTGGAATCAATCAGAATTTTCACAATATCCAGATAAAACATTACGCTAAGGAAAATAATTAAACCAAATATTACGAAATATTTTAACACATCTGCATACACCTGTTTTGAATTTTTCTCTTTTGCCTGCGAGAAGAAAAAAGGCTCAAAAGAATATCGGAAAGCCTGTATAAACAGTGTCATAATAACAGCAAGCTTGTAATTTGCACCATATATTCCGGTCTGATAAAATGGATTTTGAGCTTCAGGAATTAACTCAGGCATAATCATTTTATCCAAATTGATATTCAGAGTGCCGCAGATACTTACGATTAAAATAGGTGAAGAATAACCAAGTATCTTAAAAAGCAATTTACGATCAAGCCTAAATTGAACCTTTAAAATATCCGGAAGTAATAAAAGTAAATTAATGAAGCTTGAGACAAATATTGCCAGAAAAATATATCCAATTCCAAATTCAGGATTCCATATTTTTGTAATTGGGAATTCAGGAAAATTATTGTAAATCCACGGACATAATAGTAAGAAGAACAGATTGATCCCCAAATTGACTCCTATGTTGATCAGTTTTAAACCGGCATAACGAACAGCCCGGTTCTCCTGACGTAATTTTGCAAATGGTAAAGATGTGATGGCATCGAAAGCCAAGGTTAATGCCAATAAAATAATATAACTCTTGTTTGCCGGAATATCCAAATAAAGTGACAGAGGATTTAAGAAGCTTGCAATTGCAATCAAAAACAATACACTTGTGACTATTAAGGAAATAAAACTTGTCGAGAAAGTCAGATCGCTCTCCTTCTCCTTGCTGGCAAATCGAAAATAGCCCGTCTCCATACCATAAGTCAATACAACTAACAGTATTGCTACATATGACATTAAATTAGTGACAATACCATACTGTTCCGGGATAAACAGATTCGTATACAATGGTACCAATAACCAATTTAGTAATCGGCCGATTATAGAACTGGCGCCATATATCACGGTTTCACCGGCAAGTTTTTTAAGTGGATTCAAATCGAGCGAATTATTGTGTTAATGGTTGGCAAATATATGATAATTTTATTTTCCATTTTTTGAAAGTGTCTGAAAATGAATACATAGATGTTCCACGTGGAGCAATTTTTAATTTTCGATCAAAAAAAAGACCAACCGGTATTTATATCAAAACTAAATAAGGCTACACAAACGTTTTCGGTGGCTGTGTTTGATAATTAAAGTCTTGTAACCGACTCAAACACTTTTTTATACCGATTATTTTTTTCACTTTTGTTGCCATACGCGTTAAGGAACGTTTATGTTAGTGTTTGTTGTTTAAGTATTTTGTGAAAATCCTGGCCTTTGGGTCAGGATTCTTTTTTTATAGAAGTTTGTGTTTTCCAAAGCAACTATCGGATACTTTTACCAAAAGGTGTTAAAGCGAAACCCGCCAACTTAACATGCTGCACTGCAAATGGAATCCCAATTATTGTGATTGCAAATACCAATGCAAATAACAAATGGGTTATCGCAATCCATATTCCTCCGATTACAATCCATATCACATTCAATAAGATACTTAAGCATCCCCCTGCGTGTTCGTTGTACTCAACTTTTTGACCAAACGGAGCCAATCCAAGTACTGACAGCTGAATGATTTTCAATCCGAAAGGAATACCAACGATAGTTAAACACATTAAAAAACCACAGATAATATATTCTAAGAAAATGAATATTCCTCCAAAAATTATCCATATAATATTGCCAATTAAACTCATAACTATATTATTTATATATATTAAAACTGATATGATACTCCTACCTGCAGAACAAAATCAGGTGCTGTATTAACAATGATGTCCTCCACAAAGGCAAAGGATATAGCTAAATCTTTTGCAACAAAATAATCCAGTCCGAGAACCAATTGTGCAGATGCCTTTCCTAATTGTTTGGTATATGACTTTTCGTACAGACTCGTATGAAAATCGAGTTGTGACTTTAAATACCAATTATCGTTCGCATTAAAAGCCAAACCAATACTTCCAAAACCAACGTTACGAGTTACTTTATTTGCTAGCAATGCTCCTGTGCCAATTCGTAAGTAACCTCCCGAATAGTAAAAGGAAAACTCTTTCCTTTCAGGAGTTGAATTAATCATTCCTGTAATCTGAAATCCAAAATCGCTGGTTCCACTACCCACTAAATTTGTTTTATTTCCGGTTGGCAACTTCAAGAAAGAACGAAAAGAAACATCGTGATTTGGATTTTGTACAATGGGTGTTCCCAAAGAAATACTAACATCTCCAAAACTAAGTTTACTTTCACTCATTTGGAAAACGATCTCTTTTTCTTCCATAAAAAAGTAATTTAAACTATAATATGGCATCAAATCCCTTGACTTCCCAGGTAAATTAAAAGCATCGTGCCAATTACTAATAAAAGAATCCATAACCCCACTGGAATGTTTAACCACTGGAATAGCTATACCAATTTCCAACTTCGAAAACAGACCGTAACTTAGAAATAGCTCATTTCTGTACATTTCTCCATCCAGGTATATGGCTTCTTCATTTATTTGAGCACTTGTAGCATTATTAGCAATGCTAAAATAGTTACCGAACCAGAATGCATTTTTCTCGAGAATTGAACCTCCTGGATTGGTTGGAATCCCAAAAAAATGAACCAGAGGACTTTGATTATGAGTTTGAAACGGTTTCACCAATTTCTGACCAAGAGCGTAATTAGTGAGTAATATAAATCCAAGAAAAAGAAGTACCTGTGGCTTTCTCATGTGTGTTTCTAGGCAGATTAATTTGATCAAAGGTATCAAATCTATTAATTATTCCACAACTTTCCATACCAAAGCCGCGGCTCCCATAACACCCGAATTTCCTTTTACTGAAGAAATTTTTAATTGTGTTTTTCCCTGAAAGACAGAGAGTAGATTTTCTTCCATATGGTATTGTGTTGGCTTTAAAACTAAATCACCTGCTCTCGATAAACCTCCTGCCAGAAATATAGCTTCAGGATTATTTAATGCAACAACATTTGCCAATGCAATTCCCAAAATCTTACCTGTAAACTCAAAAGCTTCCAATGCAATTTGATCCCCGGCATAAGCTGCGGCAGCCAATCTTCTAGCACTCAAATTATTGAATGGTATATTTCTCAAATCACTTACACAACAATATTTTGCAAAAAGCTTACTTACAGTTCTTTTAATTCCGGTAGCCGATACATAAGTTTCCAAACAACCCAATCTTCCACATCCGCAATGCCTGCCTTCCGGACGAACAATCATATGTCCCATCTCACCGGCAAAACCAGTATGACCATATACTAATTCACCATTTACAACTATTCCAGATCCTAATCCTGTTCCTAAAGTTAAAACCATAAAATTTTTCATTCCTACAGCAGCACCAAACATCATTTCGGCCATAGCCGATGCATTGGCATCATTTGTAACGAAAACAGGGACATTAATTTTTTGATTGAGAAGTTTACCCAATTCAAATTTACCTTTCCAGGGTAAGTTGGGAGCATTTTGGATGCATTGGTCAAAATAATTTGCATTCGGAGCTCCCACTCCAATTCCAATCAACTCGCAATTTAATTTATTGGTAAGTCCGTTTATCCTGTTGCACAACTCATCAATAAACACATCAAATAATTGGTATCGCTTGGTAAAAATAGAATCCTCGATAAGGATACTTCCATTTTTATCAACCAAACCAAAAACTGTGTTCGTTCCTCCGATGTCTATTCCAACAGCAAGTCTTTGCATAAAAAGTGATTTAATAAAACTACAGACGTTCCTTAATCAACGATCGAATTGGATTAAAAATACGTTGCAACAAGCGATAGTCTTCTGTAATTATTTCGGCCTGACCTTCAATTTGCTGTGCAAAATCCAACTCAATACCATAATTGGTCATTAGGCCGTTTGGAAAATCAACATCCAAACTGTATTTCTGATCTTCAGGTACTTTCGATATGGAGCGAATGGTTCCTTCCAGCATCCCGTATTCAAGATAAGGATAATTATCAAGTTTTATATTCACCCTCTGTCCAATTTTGACCTTGCCGGCTCCCTGCACTCCCAATTCAACTCTGGCAATAATTTTAGTAGAGTCTGAAGGCAATACAGTAAAAACCCTCTCTCCTGTCTTCACATTTTGATTGGAGCTGTAAAAACGATTGAAACTAACCTTTCCATTAATGGGAGTAATTAATACATACTTTTGTTCCCAAATGTTGATTTGAGCTTTTAGGTTATCGAAGGATTGAATGAGCAAATTCTGCAATTCCTTTCGCTCATTTTCCTTATTCAATTCCAAATCTATAATTTGTTGATCCACTTCAGAAATACTAATCTGAGTAGAAGCCAATGAAGAACGGGTACCCTGAAAAGCATAACTCTGTTCCAAGTATTTTTTACGGGCATTTTGAAGGTCTGATTGCGAGATTACACCTCGCGTAAATAAGGATGAATCCCGGGTAAACTGCTTTTTTGAAATTGCCAATTCACTTTCAGAAATTACTTTTTGTTGGTATTGTCGATCGTAGTAAAGGTTGTATTTACTAATTTGATCACGGTAAGAGGCAATTTTTCGATCGTAATAATCCAAATCGAAAAAACGTTGGTAGTCAACATACGATTTTAAGAAACCATTGTAGTACGATTGTATTTCACCCAAACGAAAATTACCTTCCAATAAATTGGATTTAATGCCATCAACACCTGTTAGATGTTCATGAAATTCACTCATTTTCGTTTTCAAAACAAAAACATCATCATTAACGGCCGGATTCTCAATAATAGCCAAAAGATCTCCTTTTTTCACTTCCTGATTATCTGCAACATACAGGTTTTGAATCTGTCCCGACGATCTGGCAACCAATTCGGCAGGTGGATTCAAGGTTGTAAGAACCAATTTGGACTGTATAATATCCGGATAACGGAAAAACCAACTCCCAATTACCAAAGCAAAAATAACGACAAAGAAAAGAGTTGTTCCACTGCGAATGATCCAATTGGGCATTCGTTCCAAAATCTCTTTTACTTCATCTGATCGTAATTCTATGTTACTATCCATCAATTAATAATTAATAATGAGTAATTAATAATTAAAACAGAACTCTCAAGCTCTCATCTATTTATCCTGACTCAAATTACTTTTACTCGTTTCTATAATTTTCGACAAAAGCTTTAAAATCTCTTCGCAATCCAGAATTAAAGATTCAAACATTTTATCGTCAATATATTCGGTATCTTTTAATAATCTTAACCAATAATGTGTTTCTCGTGCTTCCTTAAATGAGATATGTAATTTGGCAATAAAATCTTTTTTAGATATTCCGCCTAAAGCTTCTTCAATATTTGCACCAACCGATGTCCCACATCTTAAAATTTGCTTTGAAAGAACATATTCATGCTTATTACTCTTTAAAAATTTATATAAATTAATAATACGAACACTAAATTGATAAGATTTATCATTAACAGGGTTCGGTTTCATATTAAATTTTTAATTATTAATTCCCAATTATTAATTAATTTCTAGTTTCCCAGCTCAAGCTGATTCTTCACCAATTGATAATAGGCTCCCTTTAATTTTGTAAGATTCTCGTGAGTTCCCTCCTCAACAATTTTTCCCTTATCCAAAACGATAATTTTATCGGCACTTCGAACTGTACTCAATCGATGGGCAACAACAACAACGGTTCTTCCTTTATTAAATTCATCCAAATTATTCATGATTGCCTTTTCGTTGTTTGCATCCAAGGCATTGGTCGCTTCATCGAAGAACAAAAATTTTGGGTTTTTATAGACTGCCCTGGCAATTAAAATCCGTTGCTTTTGCCCCTGACTTAAGCCATGACCGTTGGCACCAATTCTTGTGTTGTACCGCAATGGCAGACTTTCAATGAACATATCGATACAGGCAACCTGAACGGCATACAACAATCTCTTTTTATCAATATTCTCAACTCCAACGGCAACGTTTTTAGCGATGGTATCCGAAAAGATAAAACCATCCTGCATAACCACACCGCAATTCTGCCGCCACATCGATGAACTGTAATTTTCGAGAGTGACTCCACCCAAGGAAATCCTTCCTTTTGTAGTTGGATAAAATCCCAACATCAGCTTAATTAAAGTAGTTTTTCCGCTACCCGATGCACCAACAATTGCAGTGGTTTTTCCATCTTCTATCTTCAAATTAACATCATCGAGAACCATTTCGGAATGTGGACCTTCGTATTGGAAGAATACCTTTTCGATGTCAATGGCCTTTTCTTCGGGAATCTCTGTTAAGCGATCCTCATTTGGCATTTCTTCCTCCTTCATCTCGTGAATTTCACCCAATCGCTCGAGCGATATTTTAGCATCCTGCCACGAATGAATAAACTGCACCAAATTATCAAGCGGGGAATTCAATTGACCCAAAATGTACGACACCGCCAACATCATACCCAAAGTCATGCCGCCATCGAGTACCGACTTGGCCGCTAAAAAGGTGATGATGATATTCTTAGTTTCGTTGAAGAAAATGGAACCTGAAACCTGATACTGATTCAAAGCCAGACCTTTGGTTCTCACTTTAAACAATTCAGCTTGTATTTGCTCCCACTCCCAACGCTTTTGTTTCTCGCAGTTGTTCAGCTTAATTTCCTGCATTCCGGTGATTAACTGCACCAATGAATTCTGATTGTTCGAAAGTTGAGCAAATCGTTTTCCATCCAATTCTCGGCGCTTTCGCAGAAAAAGATTCACCCAAATGATATACAAAAGCGATCCAACTACAAAAACAAAAAAGATTTGCATGTCGTAAATGGCCAAAACAATTCCAAAAATCACCAAGTTAACCATCGAAAACAGGATGCTTAAGGTTGATGATGTGAGGAAATTTTCGATTCGGGTATGATCTTGTATCCGCTGCAGCAAATCTCCAATCATCTTGATATCGAAAAAGCCAATTGGCAATTTCATTAACTTGATTAGGAAATCGGAGATCAATGAAATATTAATTCGGGTTGATATGTGTAAGAGTATCCAGCTTCGAATGAATTCCACAGACATTCGCGAAATGAATAGTACCAATTGGGCAATCAGCACCAAATAGATAAAACTCAGGTTCTGCGTATTGATACCTACATCGACAATGGATTGGGTAAGGAATGGAAAAATTAATTGCAGCATACTGCCAAACAACATTCCCAATACCAACTGCACCAAAAATTTCTTGTAAGGTCTCAGATATTGAAGCAAAAAAGTAAAACTCTTTTTATGGGAAAGATCCTCGTCGCCCTGAGCGTAAAAATCGGGTGTGGTTTCAACCAACAAACACAATCCTTTTAGCTCTCCCTGACTTTGTGTACTTGCCCACGACTTAATAAATTCCTCTTTGGTAAATTTCAACAATCCGGAAGCCGGATCGGCAACATGAACCGTTGTTTTTTTCTTTTTTACCTCTATTTTGTGAACCACCACAAAGTGATTTTGTCCCCAATGGCAAATAGCTGGCAGTGCAGCGTCGTTTTGGAGCTGCTCGTAGCTGATGCGCACCCCCATGGTTCGCAGGCCAATGCTTTCCGCCGCATCGGCAATGCCCATCATCGAAACTCCTTCGCGGGTAATGTGCGATTTATCGCGTAAAGATTGAAGGCTGAAATTCTTACCAAAGTGAGCGGCAATCATACGCAGGCAAGTCGGGCCACAATCCATGGCATCCAATTGGCGGAAATGGGGGAATTTTTTCTTCAAGGCAGAATGGTAATTAGTCAATTTATTATCGTGAAGCTAAAAATAGGGAAAAATGTTGGAACGGACTAAACAGTGAATTTATATTAGTCACTAAAATGTAAATTCAAAAAATTTGTTTTTATACTCACCCCGTTTCCGGGGATTATTATTGATATAATTTGCGATTCGAAGGTATTCCTGATCATCGCGAATGATGTGATCGTGAAATCGTTCCGGCCATTCAAAATCAGAATCTATTTTTCGCGCCTCTTTTCTTACGGCCGATTTATAGGATCGGAGGATGCTAGCCAATGATCCTTTTTTTAGGGGATATGGCCGCCATTTTTTTATTTTTGGAATCCGATCCATCCTTTACAGACGTTGCATGCAACGTCTCTATCACGCATTTTGCAATTATTCATTAAATTTATCATCGTCCCATTTCCGGGGATTATTATTGATGTAATTTTCGATTCGGTGGTATTCCGGCTCATCGCGAATGATGTGATCGTGAAAACGTTCCTGCCATTCAAAATCAGGATCTATTTTTCTCGCTTCTTTTGTTACGGCCGATTTATAGGATCGGAGGATGCTGGCCAATGATCCTTTTTTAGGGGATATGGCCGCAATTTTTTTATTTTTGGAATCCGATCCATCTAGCACAGACGTTGCATGCAACGTCTCTTCTGCAATATTCGATATTTCGATAATGCCATGCACATGATTAGGCATCACTACAAAGGCGTGCAATTTTACAAATGGGAAATGATTGGGAATCCCGAACCAAAATTGATGGGCCAGCTTGCCAATTTCAGATAATTCCATTTTCTGATCAGTAATAGAACCAAAAAACCACTGCCTGTTATGACTACAGATGGTCACGAAATAGCTGCCTTCGTTGGAATAATTCCACCACACAGCACGAGCCGAGGGGATTCGGTATTTGTTTTGGAATTTTTTCATTTTAATTATCTAATGGTAAAGTGCCCCATGAAAGTAAATATATTCCCAGATTCACCCAAATTAAAAATGCACATTAATGTTAATTGAACACAGTGTTATGGGTTAACAGAAATAAAAAGATCAGTGTTAATAATATTCGTAGATAATATTTTAAATTCGAATAGTACCTATTTGATAATTAAAGACTCTATACAATTGATCTGGTTCTTCGAACGTACTCGTTATTAATTGGTAAACTCGAAATCAGTTACTAATTGCTTTGGAAAAAACAATACAAAAAACCCCATCCAGGAAACCAGATGGGGTTGAAATATTTTTTAAAAACAGAGCTTAAACGATACCGCCCACTTTTTTAAGTGCTTTACTGTTTATAATTTTAATTTTACGCCCGTTAAGTTCAATTAACTTGTCGGATTTAAACTCCGACAACAAACGAATTACCGATTCGGTTGCCGTTCCTACCATATTGGCAAGTTCTTCGCGGGTTAGAGCTATTTTAAGCGTACCCTCCGCGCTTAATCCAAAGGTTACTTCCAATTGAATTAAAATCTCGGCTAAGCGTTCGCGTACGGTTTTTTGAGCAATATCAGTAATATAGTCGTTTGCTTCACCCAGTTCTTTGCAAGTGATTTGCATTAATTCCATCGCAAACTCTGAATTTTCCCGTATCAACTTTGTCAATACATTTCCGGGTATAAAACAAACCACTGCATCTTCTATCACTTTTGCTGAAGTGCAAGCACGCTCTTTACTCAACACAGAACGAAAACCGATTAAATCGCATTCTTTTGCAAACCGAATAATTTGTTCCTTCCCTTCCAAACCTGTTTTAAAAACTTTTAAAACACCCGAAAAAAGAAAATAACATCCTTTGGCCGAATCACCTTCCGAATAGATAATACTACCTCTTTTATAAAAATGAACATCTTCATCCCAAAACAATAGATTCTTATCTTCTACCTTCAGATTTTGAAATCTTTCACCGAACTTCTCGGCTAACTGATTGCAATTTGGAAGAATTGGATTCTGCTTCATTAAATTCAAATTAAAACTGAAATCAATACTACTAATTCAATAACAATTCCTTTTCTCGAACTTCCACACACAACTAAATGTTTAAAAAATTATTTGGTTCGAAGGCTTTTTCCTACTTTTATTACAAATCGTTTTCGATTTGTAATAAGGTCAAATATACAAATTTAGTTATACATATAGCACAGTTAAAAGGACAAAAATACAGCAATTATGAAGCACAAAGTAGAACTATCATGGCTGGGAAACCTTGCCTTCGAAACAAATATGGATGGTCATAAAATGATTACCGACGCTGGAATTGAATTTGGCGGTGAGGATAAAGGTCTTCGGCCAAAAAAGCTGATGCTAACCGCATTAGCAGGGTGTACGGGCATCGATACGGCGTTAATTGTAAAGAAAATGAGGCTGGATATTACAGATATTAAAGTATCGGTAGAAGGTGAGTTAACCGAAAGTCAGCCCACTTACTATAAAAATATGCACATTACCTATGAATTCTTTGGCAAGGATCTACCTCATAAAAAATTGGAGCGGGCCGTTAAAATTTCCGAAGAATCGCAATGCGGCGTAAGCGCAATGTACAAGCAGGTAATTCCTGTTACTTCCGAGATTATTTATCATGAAGAGTAAAAAACATCTTGAAAGGTCTGGGAAAGTCGAAGCGTCCAATAGTCGAAAGGTCAAACAGTCCTTAGTTTTCTAATTAAGGTGTAATTGCAAAAGAAAAGCTCATTTATTCTTTTCCCCCTGGCTTTAGACCGGGGATTAATAAAAAAGGAAAACTTGAAAAGTGGAAGAGTTCAACAATCTAAAGGTCAGACAGTCCGTAGTATTCTATTGAAAAACTCGTTTATTCTTTGCTCCGTGGCTTTAGTCCGGGGATTAATAAAAAAAGAAAACTTGAAAAGTCGAAGAGTCCAACAGTCAAAAGGTCAAACAGTCCGCAGCATACTATTGAAAAGCTCATTTATTCTTTGCCCGTGGCTTTAGCCCGGATTATCTAGACAAAAGAGTCTCGAATAGTCCAATAGTCCAATAGTCCAAAAAATAAAAAAGTGCCTCTCCGAATGAAGGGCACTTTTTATTTTTATCGTGTAAGATTATCTCAAGTCTAAAAACTCTATCCGTTTTCTAGAATCCTGCGGCAGAAAATTGTTCCAGGAAGCGAATGTCGTTCTCGAAGAACAAACGCAGATCTTTAATGCCGTATTTCAGCATGGTGATTCTTTCAATTCCCATTCCCAATGCAAAGCCTGTATATTTTTTATTATCAATTCCATTTAATTCCAAAACATTCGGATCAACCATACCGCAACCCAAAATCTCTAACCAACCGGTTCCTTTGCACACATTACAGCCCTTTCCGCCACAAAGTGAACAAGTAACATCAACCTCGGCCGATGGCTCTGTAAACGGGAAGTATGATGGTCGCAATCTAATTTCTGTTTTCTCTCCAAAAAATTCTTTCGCGAAATAAGTCAATGTTTGCTTCAAATCGGCAAATGATACGTTCTCGTCGATATACAAACATTCAATTTGGTGAAAGATACAATGTGCTCGTGCCGAAATAGCTTCGTTACGAAAAACACGACCTGGCGTTAAGCAACGAATTGGCAATTCCATTTCAGACATATCATGCGCCTGAACCGAAGAGGTATGTGTACGCAAAATTACATCCGGATTTTTTTCGATGAAGAAAGTATCTTGCATATCACGTGCAGGATGCTCATCAGGAAAATTTAATGCCGAAAAGTTATGCCAATCATCTTCAATCTCCGGACCTTCGGAAATGGTGAATCCCAGGCGAGAAAATATTTCGGTAATTTCATTTTTCACCAAAGAAAGCGGATGGCGGGAACCCAACTTAACCGCATCTCCCGATAAAGTCAAATCCAAACCTGACTTTCCAAAATCAAGATTGGTGAAACTATCTTTAAGAGAATTTACTATATCCAAAGCAGTTGTCTTCAACTCGTTAAGAGCCTGTCCGACAGCTTTTTTCTGCTCGTTTGGAACATCCTTAAAATCTGCAAACAAAACAGCAATACTACCTTTTTTACTAAGGTGCTTGATTCTAAATTGTTCAACTTCCTCTAGTGTAGTTGCCGAAAATCCATTCAACTCCTCCAATAAATTCTTTATTTTGTCTAACATATTTTTAATCTTTTGAAATCCGATAGTCGGCAAAGTTACAAAAAGTTTTTTCTCCTTGTTACTGAGGATTCTATTTTAGACTGAAGATTCCTAAATTATTTAGTATTCCAGAATTTTCACTTTCATTTTTACATCCTGAAACGGTCGGTCATTTTCATCTGTTTCCAAATCAACAATCTTATCCAAAACATCCAATCCTTCTACTACTTCCCCAAAAACAGTATAATTCCCATCAAGATGCGGAACACCGCCAATCGTTGTGTAGGCTTTCCGACGTGCCTCCGAAAATTCCAGTTTTAAATTGGAACTATTGTATGCAAATTCAATAGACCCCTTAATCGTCTTGGCAATGGCTCTAAACTCGTCAACTTTATCAGCTTTTTTCAAAGAATCCAAAACGGCCCTTTTATCTAAAGTTAAATACCGTTTAACGATTCGCTTTCGCAAAGGAACATTCACTTCTTTCTCCATCAGTGTCAATTCCTCATCAGAAAAAACTCTGCCGTGAGCAATATAAAATTGAGAAATATCCGATTCTTTAAAAATATTCACATTATCGGGCTTTCGAGGTGCAGCAATAGCTCCCTTTTTATGAAAAAACTTTTTATTAAACTCTGATTCAATGGTTCTGTTCGAACTACCATAACCAATCATCTTTCCTGCCGGTGCATTTCTGGAATCCTGCGAGCCACCCTGAGCCACAAAACCCTTTATCACCCGGTAAAACAGCGTTCCATCGAAATGACCGTTTTCTATCAATTGAAGAAAATTCTGCCGATGCTTTGGGGTTTCTTTGTAAAGCATCAATTTGATATTCCCCATATTGGTTTCAATCAAAATAATCGAATTCGTAGCTTGCCCAAATGTTAGCTTAGTGATTAGAATAATTACAATTGCTAAAAATACTTTCTTACTGAAATTCATTGATTTCCTTCTTTTAAAATTTGCACTAAGATAAATATCATGCGATTGAAACCAAAAAAAGCTTTGCATTCATTGCAAAGCTCTTTCTTCTATCATTAAAACAGCAGGTTTATTTTAAAATTTTAATTTTCATTGATATGTCTTTCAATGGGCGATCGAAATCATCTTTCTCAACTCTTGCTATGGAATCAATTAAATCCATTCCCTCGATAACTTCACCAAAAACCGTGTAATTTCCATCCAAATGAGGAATTCCACCAACAGTAGTATAGGCTTCAATTTGCTGTTTACTTAAAACAATTTTCTCCAAATTAAACATTGAATCAACTTCAAATTTAATGTGATCAATTGCCTCTGTCAACTTCTCCAACTCCCCTTCCATTTGACATTTGGAAAGAAATTCAGCTTTTTCCATTTGCACCTTCTTGAAAATAGAACTCCTTAGTTTTCCGTTCAGCTTCGTTTGTAAAGTATCCAATTCACCAACAGTGTAAACCTTTCCTTGTACAATATAAAATTGGGAACCCGCTGATTTCTTTTCCGGATTCACATCATCACCTTCTCGTGCGGCAGCCAAAACCCCCCGCTTGTGAAATAAACCGTTTTTAATTTCTGCAGGTATCTGATAGCCCGGACCGCCTTCGCCCAATCGCTCACCTGATTTTGCACTTTTTGAATCAGGATCCCCACCTTGAATCATGAATCCATCTATCACCCGATGAAATAGAGTACCGTCAAAGAATTTATCATGAGTTAATTTTACAAAATTATCTCGATGAACAGGCGTTTCATCATATAAACGAATTTTCAGATTTCCATATTCCGTTTCGACTTCAACAACTCTATCTTTTTTACCCAGCTCCAATTTTTTCTGGCAGGATATAAAAAGAAAAACAATAAAGATGTAAATGAGATTTTTCATAGGTAATGATCAGTTTTTTTAATGTTTGTATCTAGCGTTTATTTTCAGTTTCTAAATAATCGTTCTCTTTGAAATATTCTACAATCGAAGATTTAAGTAATAACTCTTCCTTTTCCTTGGAAAAAGGGGGAACCATTTTATTCTTTTTGAAAACCGGCCATTGATTTTCATCATACCCATCAATTTGATAGTAATTCCACTTCATCAACAAAGTACAAGTTGCTAAATTGATATGATCCATCTTTTCGTCCTTTTCATACTTCTCCTGAAAGCCTTTTCCTCGCTCCTGTATCCCAATAAGTAGTAAAGTTCCATTTAAATCGGCATCCACATTAAAATCTTCAGAAATTTTATATAAAATTTTGTTCCAGTCGCTTTCTAAATTATTATCCATAGTATCAATTTTTTATGAGTGTTAAAAGTAAAGATTTCTGTTCATAATGGCACCTCTAAAAAATATATTTTCACATTTTTAATTATTCTACTTGGAGATAAAATAATTCCATCCTATATTTGCATCGCTTTTAAGGGCAAGGGCGATTAGCTCAGTTGGTTCAGAGCACTTGGTTTACACCCAAGGGGTCATAGGTTCGAATCCTATATCGCCCACTATAAAAAGCAAACTATTCCCAATATTCGGGCGATTAGCTCAGTTGGTTCAGAGCACTTGGTTTACACCCAAGGGGTCATAGGTTCGAATCCTATATCGCCCACGAAAAGATCAGATTTTCTAATGAAAATCTGATCTTTTTTTTTACCATAAATTGTCCATTCATAAATCTTTTTATGACATCTACCGTATATAAATTCAAATTGATTAAAAAGTCAAACTTTTAGAAACATTTAAGTTTCTAAGAAAATTGTATATTGCGTCGATTTATGTTAAAAAATCATCATTTTTATTTAAAATCATCATTTTTATTTTAATTTTATTAATCACTTGATCAGTCACTTACACAGAAAAGTGCACTTTTTTTACTTTTTTTTACTTTTTGAGGGTTACCTTTTAGTCAATAAGGGAATAAAGGGATAGGAAATATGGATTACACTACGGAAGCGATACTTGAGGGAATCAGCATGAGCAATAATGATGTATTAAATTACATCTACAGCAAATTCTTCCCAAGTATTCGAAATTATATAGAAAACAATAACGGCAACGAAGAAGATGCAAGAGATCTTTTTCAAGAGGCCATTATCGTAATCTACCGTAAAATGAAAAAAGAACCGCTGGTTCTGAATTGTAATTTTAAAACTTATATCTATTCTGTTTGCAGACTTTTATGGTTGAAGCAACTTGAAAAAAGAAAAAATAGCAATGAAATTAGTTCAGAAGCTATTTTGGACAATAGATTGGATGAAGCAACAGAGACCTATGATCAAACCGAAAGGTATAGGCTGTATCAGAAACACTTCAAACTTCTTGGTCCTGATTGCAAGCAATTGCTTCAATTAACTCTTGAAAAAGTTTCCTTAAAGGAAATATCCGAAATTATGGGATATAAGAGTGAGAAGTATGCAAAAAAGAAAAAATATCAGTGCAAACAAACATTGATAGAAAGCATTAAAAGTGATAATGAATTTAAAGAAGTATATGATGGATGACATGAATTTTGACCGTATCGAGGATTTTCTTGACGGTGATCTGACAGAAAACCAATTAAAGGAGTTCGAGAAAGACTTACTCGAAGACTTGGATCTTCAAATGGAGCTTGACCTTCACACGGAGGTTAATGAGGCCATAATAGAACAAGACGTTATGGATCTTAGAAGTAAATTAGAGGCGATTGAAACCCCTCACAATCCTGCTGAAAAGCGAAAGCTGAAATTTTTGACTAAGTGGAATATTACAGCGGCATCTCTGGCCTTAGTAATTGGTTTGGGAAGTTTAATGTATATACTAAACAATCCATCTACTTATTCAAACGATAAAGTTTTTAGCAATTACTATAAGCCTTACAATGTTGTTGTCAACACTCGTTCGTCAGATGTCATGATTGATAATCTATTGGTAACGGCTTTAAAAAGTTATGAAACTAAAGATTATCGAACTGCATTAACTTTATTCAAGAAAATACTGGATAAAGACAGTACGAACATCACCGGTAATTTTTATTCAGGTATCTCGAATATTGAAATTAATGAATATTCAAAAGCGAATAAGAACTTCACCAGAGTGCTAAAGCATAAGAATAACCTGTTTATAGAGCAATCTGAATGGTATCTTGGTTTTTGTTACCTCATGACCAATGAGAAAGAAAAAGCTCTTAAGCAATTCCATGTAATTGCCCAGGGAAATAGTTTCTATAAGACGAAAGCTTTAGAAATAATCAACAGACTGGAATAGATCACTTCATCATTCATCCAAATAAAGTAAGCCATCCAATTAAATTGGGTGGCATTTTTTATGATGTTAAAAGCTTAGCAAAAAGCTAGTAGGATCGAGAAAATATCACAACACAAACTGATAAGTATGACCTTACAGAGTGTTTATTCATATTCCGTAACTTCTATATACTGAAGTAGAATTGAAGATACATAAGAAACAAAAGCAGTCTTAATAAATTTAAATACACTAAGCTTATGTTTTTTATCGTACAAAAAATGGGTTTAATAATATTAGGCGACTGATTTACGTCTTAACTTCTTTCGGAATAGTAGTAGAAAAATGATTCCAACAGATCCAAATGCAAAATACATTAGTTTATTTACCAGGCTATCATCGTATAATGGATCCACATCACCTAATGTCACATATCCCGACCAAAAATAAGGATGGGATTTTAATGAATCGGCAGTTTTTAAATACTCCAATTTTGCTTGACGGAGCGCATCATCTTTGTGCAAGCCTTGAGATAAAAAAGTATAGAAGTTACTCATTAAATTTGATCCTGTCTTGTCCTCTACAGTCCACAAAGTCATGATTACACTTGGACAGCCGGCATAAAAGAAACCTCGCGCCAAACTCATTACTCCTTCTCCTTTCAACAACTTACCATCTCCCGTGTTACATGCACTCAAAACAACCATTCTTGCATTAAAATTCATGTTATAAATTTCATGCGTATTTAAAAGACCGTCTTCAATTGTATCTGAATTTTGCGTAAAAACCAATTTGGAATACATCGGATTTTCATCATCAATTATGGTATGCATTGCCAGATGTAATACATCGTAATTACTTGCATTTGCTTTAAAATTAGCTTCGGTGGCCAAATCATCAACAAACAAATCTCCATGAATCACATTTGAAATATTATTAACCTCTTCCTTAACACCAGGCAATGGATATAATTTATCGCGGTAAGATCTTTCAGTAAATAATATTGAATCATTCACACCTTCGTAAGAAGGAGCAAAAGCCAAAACCGATTTGGAAGAAAAAACACCATAACTAGTAGAATGATTTTTAAAACCCAAAGTAGCCGAATTGTGATATGTAATTGTATTCTCTTTTATTAAATATTCCAGGTTTCTATAATTCATTGTTGTAGAATCGGCTTCCTTAGTTAAAAGAACACCAAACGGAATGTAAGCCATTTTATCATCAGGAACTATAAGAAGATTTTTACCTGAAAGCAGACTATCTTTTCCACTCAATAAATACTGATACAATTGATAAGCCGATCTTGTATATCTTTTATAATAATCAGCACTATTATTAGAAAAATCATTCGTTTTTAAACAATTACGAACTTCCTCAAGATGATAATCGAATGTATCTTTTACTATTTTTTGTCGCTCTAATTCAAAACTTGATTTGGAAATAATAAACGAAAATAAGGCAGAATCGGATATGGAATATTCAATAAGCACATCATTATCATCTAATCTACTTTGTAAGTCCTCAATAGTAATGGTTTTGGTATCATACTTTAAGGAATAATACTCCGGATAATCTTCCTCAAAATTCAAAACCATTTGATTGTATTCCTCATTTAAATTGAAAAGTACATTTTGCCATCCACTAATTGAATCACGATTTGGAGTTTGCTTTCTTTTTTCATCATGAACGAATTCACGGTATTTAGCAATATTCAGTTTTAAATCTCTTTCCTGCTCCTGCAATTCGATAGAAATACCCCCAAAATTTTTAGCATTTACATCATTCAAGGAAGATAGTAAACTAGCCGACTTTGATCGCTCAGCGTATCGAAAAGCTTTTTCTTTGTACTTTACATCTTTAGTAAGTTGATACAATTCAACAGCGATTTCGATAGCTTCGGTATACGTATCTTTTTCGTTTTTAGACAAAGCAAATTTGCTCTCTTCGTCCTGATAGGCAATTCGAATGTTATCAATTATATCCAAACAAAGATCATAGGTCTGTAAACTGTAATCCAAATCAGGAATACTATTAGTCTTGCTATATAATTCCTGTAAAGTATTTGCTTTGGGTTTTAAAATTTCTAAAATGGAAAGCTGCGGATCCAATTCATCCATGCTAGGATTGATATAAACAGTGCTGTTGTTAAAATTATCCGTTTCAGCAATTAAGGCCTTTTGATAATACTTTAAAGCATTTAAATGATCATTTCTTTTAGAATAATAATCCCCAAAATTCTTCAAGCAATGGGCAGTATCCGGATGCTTTCCTCCAAAGTTTTTTACATATACATCATATGCAAGGTTTAGATAAGAAAGAACTTTATCACTATTTCCAATTTGATCCTGAAAAACAGCATAATTCAAATAATAATCGCCTAATAAATAGAAATGAAACCCATTTATTTCTTTTACTTCAGAAATACTTTTTTGATAGTAACTATCAGCTTCTTCGTAATTTCCTAACTTTTTATAACAATTAGCAATAATATTTCTTGTAGTTGAAGTATTTCCTGTTTTTTGCTTTGTTTTTATATTTAATGTTTTCAATAAATACGTCAGAGCTTCAGAATAGAGTTTCTTTTTATAATATATTACTCCTAGGTTATGATATAATTGCGATTCCCATTTTGAACCATTTACTATATCAATCAGAGATAATGATTTCTTATAATAATTTTCCGCCTTAATTAAATCTTCATAATCATTATAAATATTTCCTTTGTTAATGTAAATTGGAATTAATTTATTTGAATTTTGACTATACTCAGCAATAGTAAAAATCTCTGCTTCATTATAAAACTCTATAGCCCTTTTTCTATCTCCAACTCGATTCAGTAAAACACCAAAGTTTACGTAAGTTCTATAAATCAAATTTTTATCAGACAAACCTCCCTTATTAATAGTAAGTATCGCATTCTCGAAAGATTGAATTGAAAAATCAAATTCACCCTTCTTTAAAAATCCAACCGCTCTATCAAACTGCACTTGCAGTAATGAATCAGGGTTCACTTTTTCAACTGGGAAATGCTTTTCTAAGTCTGAATTAGACTTAGAATTAGTGCTCCCACTAAAGAGAAAGAGAAGAAAAAGAGGAAAAAGAGGATAAATAAGAATTTTTTTTAGAAAATTTTTCACGCTAGCGTAGAGTTTTATAATATTCTTATTAACAATTAATTACAAGGAAAACACAGGATTACAGAAGTCCAAATATAGACCTTTTTTTTATTTTTTATTAAAAAAATTCTGTTTTCAGGGTTACCTTTTTCAAAAAAGGGGAATAAAGGGGTGTAATCAAAAAAACTTAAACAATCCAAATCTAACAAAAACAGATAGTAATGAAAAACTTTGAAATAGTATCCCAAGAAGAATTACAAAATATCAAAGGTGGTTATAACGAAATGACAGATACATGTACTCTTGATGACTGCATCGTTTAAATAAACTTATTGACCAAATCCAAATTTCCCAAGTAAACCTAGTATTGACCAATAAGACAAAATAGCATGAAAAATTTTGAAGTAGTATCTCAGGAAGAATTACAAAATATTAAAGGTGGTTATAACGAAATGACAGATACATGTACTCTTGATGATTGCATCGTTTAATAACCTATTTTGACCAAATCCAAATTTCCCAAGTAAACCTAGTATTGACCAATAAGACAAAATAACATGAAAAATTTTGAAGTAGTATCTCAGGAAGAATTACAAAATATCAAAGGTGGTTATAACGAAATGACAGATACATGTACTCTTGATGACTGCATCGTTTAAATAAACTTATTGACCAAATCCAAATTTCCCAAGTAAACCTAGTATTGACCAATAAGACAAAATAGCAATGAAAAATTTTGAAGTAGTATCTCAGGAAGAATTACAAAATATTAAAGGTGGTTATAACGAAATGACAGATACATGTACTCTTGATGACTGCATCGTTTAAATAAACTTATTGACCAAATCCAAATTTCCCAAGTAAACCTAGTATTGACCAATAAGACAAAATAACATGAAAAATTTTGAAGTAGTATCTCAGGAAGAATTAAAAAATATCAAAGGTGGTTATAACGAAATGACAGATACATGTACTCTTGATGACTGCATCGTTTAA

Protein sequences of DBSCAN-SWA_2

>NZ_AP018042|2309575:2347311|2346536_2346638_+|WP_145957603.1|bacteriocin|DBSCAN-SWA
MKNFEIVSQEELQNIKGGYNEMTDTCTLDDCIV
>NZ_AP018042|2309575:2347311|2310210_2311194_+|WP_096429024.1|DBSCAN-SWA
MLYFISSSTNPSFNIATEEYLLKNYEDDFFFLYTNTPALIVGKHQNTLAEINLEKAEQENIPVVRRMSGGGTVFHDEGNLNYCFIKNGEKGKLVDFQKYSQPIVDTLQALNVDAKFEGKSDLTIDGKKFSGNASHIYKNKVMQHGTMLFSSDLKQLNQLLKVNPLKFKDRGIRSIRSKVTNISEYLSDSMSLEKFGQLILEDLKTKFLDAKKFELSAIDIEKIEALMAEKYNNWEWNYGYSPNYIFEKLSNFPGGGILELSMKVEKGIIKNLELFGNCLVENQISHFKTKLIGKYHDKTTIRKTLKELDLNLFFHEITDKELLSALF
>NZ_AP018042|2309575:2347311|2322305_2323169_-|WP_096429031.1|DBSCAN-SWA
MSNRIYLKTTLRVLVIIIALFQFSCKSFYKLGVEKAKTMSDNKLYSGLIDSSLNYQTLYVKRFAANFSVDGISKSFKGSIKIEKDSIIWIDISATVGIPVARILITPDSVKMIDRLKKTYLIDGFDFFSEKLNLDLDFDSFQSILTNSIFKLDNEEKEKAFIRSFNGKIIGNKYVFISEKARKIDRKLKKDKLDKLDKYNYQRIDIEPLLMRITDIFVKEFDTSREISIKYRDFTVFQNRKFPQRLNFEVKDPKHLLSCNIKFNKITFDEKLRFSFTIPDKYDRIYP
>NZ_AP018042|2309575:2347311|2329964_2330912_-|WP_096429037.1|DBSCAN-SWA
MQRLAVGIDIGGTNTVFGLVDKNGSILIEDSIFTKRYQLFDVFIDELCNRINGLTNKLNCELIGIGVGAPNANYFDQCIQNAPNLPWKGKFELGKLLNQKINVPVFVTNDANASAMAEMMFGAAVGMKNFMVLTLGTGLGSGIVVNGELVYGHTGFAGEMGHMIVRPEGRHCGCGRLGCLETYVSATGIKRTVSKLFAKYCCVSDLRNIPFNNLSARRLAAAAYAGDQIALEAFEFTGKILGIALANVVALNNPEAIFLAGGLSRAGDLVLKPTQYHMEENLLSVFQGKTQLKISSVKGNSGVMGAAALVWKVVE
>NZ_AP018042|2309575:2347311|2334986_2335187_-|WP_145957602.1|DBSCAN-SWA
MASILRSYKSAVRKEARKIDSDFEWPERFHDHIIRDDQEYLRIANYINNNPRKRGEYKNKFFEFTF
>NZ_AP018042|2309575:2347311|2316928_2317354_-|WP_096429027.1|DBSCAN-SWA
MVITYDSCETNIPAIDQERLSEWINRVVVTNGYEIGEINYYFCSDNYLLEMNREHLNHDYFTDIITFDYTVSDIVSGDLFISIDTVLDNAKEYGCDYFQELHRVMIHGILHLMEIDDKTDEDQEIMTQKEDESLALLSNID
>NZ_AP018042|2309575:2347311|2309575_2310028_-|WP_096429023.1|tRNA|DBSCAN-SWA
MRIVIQRVTQATVSVDSKIIGQISKGLMVLVGIETADTEEDSEWLSKKICNLRIFDDEEGVMNKSLMDVDGNILAISQFTLHAKTKKGNRPSYILAAKPDISVPLYENFVACLEKESGKKVETGEFGAEMQVSLVNDGPVTIVMDTKNKE
>NZ_AP018042|2309575:2347311|2328923_2329916_-|WP_096429036.1|DBSCAN-SWA
MRKPQVLLFLGFILLTNYALGQKLVKPFQTHNQSPLVHFFGIPTNPGGSILEKNAFWFGNYFSIANNATSAQINEEAIYLDGEMYRNELFLSYGLFSKLEIGIAIPVVKHSSGVMDSFISNWHDAFNLPGKSRDLMPYYSLNYFFMEEKEIVFQMSESKLSFGDVSISLGTPIVQNPNHDVSFRSFLKLPTGNKTNLVGSGTSDFGFQITGMINSTPERKEFSFYYSGGYLRIGTGALLANKVTRNVGFGSIGLAFNANDNWYLKSQLDFHTSLYEKSYTKQLGKASAQLVLGLDYFVAKDLAISFAFVEDIIVNTAPDFVLQVGVSYQF
>NZ_AP018042|2309575:2347311|2326669_2328163_-|WP_096429034.1|DBSCAN-SWA
MNPLKKLAGETVIYGASSIIGRLLNWLLVPLYTNLFIPEQYGIVTNLMSYVAILLVVLTYGMETGYFRFASKEKESDLTFSTSFISLIVTSVLFLIAIASFLNPLSLYLDIPANKSYIILLALTLAFDAITSLPFAKLRQENRAVRYAGLKLINIGVNLGINLFFLLLCPWIYNNFPEFPITKIWNPEFGIGYIFLAIFVSSFINLLLLLPDILKVQFRLDRKLLFKILGYSSPILIVSICGTLNINLDKMIMPELIPEAQNPFYQTGIYGANYKLAVIMTLFIQAFRYSFEPFFFSQAKEKNSKQVYADVLKYFVIFGLIIFLSVMFYLDIVKILIDSSYHEGLKIVPYVLLANLFFGIFFSLSLWYKLTDNTRFGAYIAIIGTIITVVLNILLVPKFGYMGAAYSVLACFFTITVISYLAGQKYYPVQYDLKRILFYLFLALVVFIASTYIGIENQWLKMAAKTPLLLLFGVVVIQKERLWGSLGKLIGVSKSRK
>NZ_AP018042|2309575:2347311|2341911_2342478_+|WP_096429048.1|DBSCAN-SWA
MDYTTEAILEGISMSNNDVLNYIYSKFFPSIRNYIENNNGNEEDARDLFQEAIIVIYRKMKKEPLVLNCNFKTYIYSVCRLLWLKQLEKRKNSNEISSEAILDNRLDEATETYDQTERYRLYQKHFKLLGPDCKQLLQLTLEKVSLKEISEIMGYKSEKYAKKKKYQCKQTLIESIKSDNEFKEVYDG
>NZ_AP018042|2309575:2347311|2330931_2332224_-|WP_096429038.1|DBSCAN-SWA
MDSNIELRSDEVKEILERMPNWIIRSGTTLFFVVIFALVIGSWFFRYPDIIQSKLVLTTLNPPAELVARSSGQIQNLYVADNQEVKKGDLLAIIENPAVNDDVFVLKTKMSEFHEHLTGVDGIKSNLLEGNFRLGEIQSYYNGFLKSYVDYQRFFDLDYYDRKIASYRDQISKYNLYYDRQYQQKVISESELAISKKQFTRDSSLFTRGVISQSDLQNARKKYLEQSYAFQGTRSSLASTQISISEVDQQIIDLELNKENERKELQNLLIQSFDNLKAQINIWEQKYVLITPINGKVSFNRFYSSNQNVKTGERVFTVLPSDSTKIIARVELGVQGAGKVKIGQRVNIKLDNYPYLEYGMLEGTIRSISKVPEDQKYSLDVDFPNGLMTNYGIELDFAQQIEGQAEIITEDYRLLQRIFNPIRSLIKERL
>NZ_AP018042|2309575:2347311|2317356_2317764_-|WP_096429028.1|DBSCAN-SWA
MTKSEKLLVFPSELENISRVEKLIDEISSSHNLSSEIYGKISVAIIEAVNNAILHGNQLDINKKVKIEYNIDDESISFIVGDEGKGFDFSNIPDPTLPENLEKTHGRGIFLMNHLADDIEFAKNGAIVEMKFIFG
>NZ_AP018042|2309575:2347311|2338004_2339027_-|WP_096429044.1|tRNA|DBSCAN-SWA
MLDKIKNLLEELNGFSATTLEEVEQFRIKHLSKKGSIAVLFADFKDVPNEQKKAVGQALNELKTTALDIVNSLKDSFTNLDFGKSGLDLTLSGDAVKLGSRHPLSLVKNEITEIFSRLGFTISEGPEIEDDWHNFSALNFPDEHPARDMQDTFFIEKNPDVILRTHTSSVQAHDMSEMELPIRCLTPGRVFRNEAISARAHCIFHQIECLYIDENVSFADLKQTLTYFAKEFFGEKTEIRLRPSYFPFTEPSAEVDVTCSLCGGKGCNVCKGTGWLEILGCGMVDPNVLELNGIDNKKYTGFALGMGIERITMLKYGIKDLRLFFENDIRFLEQFSAAGF
>NZ_AP018042|2309575:2347311|2336168_2336873_-|WP_096429042.1|DBSCAN-SWA
MKQNPILPNCNQLAEKFGERFQNLKVEDKNLLFWDEDVHFYKRGSIIYSEGDSAKGCYFLFSGVLKVFKTGLEGKEQIIRFAKECDLIGFRSVLSKERACTSAKVIEDAVVCFIPGNVLTKLIRENSEFAMELMQITCKELGEANDYITDIAQKTVRERLAEILIQLEVTFGLSAEGTLKIALTREELANMVGTATESVIRLLSEFKSDKLIELNGRKIKIINSKALKKVGGIV
>NZ_AP018042|2309575:2347311|2342449_2343220_+|WP_096429049.1|DBSCAN-SWA
MNLKKYMMDDMNFDRIEDFLDGDLTENQLKEFEKDLLEDLDLQMELDLHTEVNEAIIEQDVMDLRSKLEAIETPHNPAEKRKLKFLTKWNITAASLALVIGLGSLMYILNNPSTYSNDKVFSNYYKPYNVVVNTRSSDVMIDNLLVTALKSYETKDYRTALTLFKKILDKDSTNITGNFYSGISNIEINEYSKANKNFTRVLKHKNNLFIEQSEWYLGFCYLMTNEKEKALKQFHVIAQGNSFYKTKALEIINRLE
>NZ_AP018042|2309575:2347311|2323158_2324931_-|WP_096429032.1|DBSCAN-SWA
MKVGCKFWIAILFACMATTPVITEGAVLVPKKDKIQKEVKLTEAQKLEFDFAFTEATKAILLQELDKGLSLYATCLKIDPTSAVVRYELANIYMSQDDFSSALELSRGAVALQPLNIWYQIQLANIYQKKGMMDQACAVYDDLIELHPERKDFYYLQAAIFASVEKFDKAIEVYDRLEKKEGIQEGVSLEKQRLYLAAGNKKMAYAEIQKLIKKHPYRADFYGILADLYLADKEEGKAFDQYEKILKIDPDNGLVHFYLADFYRRKGEIEKSNVSLAKAFGNSEIEADQKIQFLISILMNGDQESMSDEYLKGLLDKLVEIHPGQVRVHALYADFLRKRKDNEGARYHLRKVLEEEKTNYVVWEELLLIDNELLDFESMLKESSEALKYFPDQPLLYIFKGVAAAQKEDYKTAIQTLEEGFSYIGDNVKLRVQFQTYLGDSYYQMGEAKKAFKSYDEVLSFEPQNVIVLNNYSYYLSVRGEELEKAKKMSSLCVDLEAENSTYLDTHGWVLYRLGEFKEAKTILEKALEFGGGESAVIVEHYGDILFRLGDSDKALEEWKKAIEIGEGSEYLAEKVKTGIIPDEIEQNEQ
>NZ_AP018042|2309575:2347311|2317756_2321050_-|WP_096429029.1|DBSCAN-SWA
MNSSQEIFIGKLDRFIRKYYQNQLLRGSILSIVLILTYFLIVSYTEYLLYFSVPVRTFLAAFSILVFSVMLIKLIILPTIGLFQIGKRISYRQAIEIITRYFPDLQDRLVNTLELTELSAKSESTKDSLLMASISQRMESIRLIPFRKAISFKSNISYLKYMLIVLTAIIGTYIFLPDMYSSSANRLVHFRKEFIAPADFKFVLDQQHMKVVRGSNFLLKVHTEGKYVPSDVYLVSEGNEFLLKKEGNNGFSYLFRNVNNDVSFYLKAGKVVSLTYQLELLLKPGIKSFELSVNSPSYTKIAERKEQNLGDISVPEGASLQWTIEGEDTNEVQLIFSYTLNHLDTKKDGDAFSFSKKFFKKNAYKILLSNQHFDKQLFANYTVDVIPDLYPQIQVSSIQDSVLTTGYYFKGILKDDYGFSKLRFCYLINDQLPIYIPITISRQLSSQEFYFAFDFATVDISEGIQVQYYFEVFDNDEVNKPKMTRSEHFSLLIPDSKQLYDLNTNIQDSISNRIEKGIDLSQSIQEDIKRLQKNFLDGTGDKWQQQQMFQQIESKKKELDELLNTIKNENLKKNQLMNSLGEKDALLLEKQKQIEKLLENVMDDELQKLFEEFNKLAEDFKSDDLNKLGNQLNMSFEDFQKQMDRNLSLLERYDIETRMSKIIERIDKIADNQEDISTLGRKQDEKMNSQQLKDTQKWKDLEKDLENLLEKNSGISDPYQFQNPSEDLQEISEMMEESENLLEEGKNAKASKNMKATSKKQKALAQKLNNNLSQSVGAQLTVDIDNLIRLLNNLMEFSFQQEALLTDYKTVDYRNPLFVKVIEEQGELKEEYLLIQDSLLSLSAHSPQVAALIGNRIFDISNYLDQVLEEANNRRKIQANITQQKVLTEVNELSLFLSEALKQLMEQMANAMPGDQIGDKKGGKPSFSGMRSEQQSLKKMLEEMINEMKSGNGKKSSSEKLGKFLQKQEMFKQSLSEMMQKGGVGDETENILREIMKMIDQTELDLSNFSINSNTVNRQNRIISRLLEAENAHREKDFDKKRESNSGNIIKLSNPIEIFEYKRVRTDFEGLFDDSYVKMLDYYNKLYLDYMIKINND
>NZ_AP018042|2309575:2347311|2326045_2326480_-|WP_096433552.1|DBSCAN-SWA
MKVKIVNKSKHDLPKYSTELSAGMDLRANLNEPVFLKSLERKLIKTGLFIELPAGFEAQIRPRSGMALKEGVTVLNTPGTIDADYRGEIGVILANLSNTLVEIKDGDRICQMVVAAHETVSWEPVEDLEASERGAGGFGHTGKS
>NZ_AP018042|2309575:2347311|2337083_2337503_+|WP_096429043.1|DBSCAN-SWA
MKHKVELSWLGNLAFETNMDGHKMITDAGIEFGGEDKGLRPKKLMLTALAGCTGIDTALIVKKMRLDITDIKVSVEGELTESQPTYYKNMHITYEFFGKDLPHKKLERAVKISEESQCGVSAMYKQVIPVTSEIIYHEE
>NZ_AP018042|2309575:2347311|2311264_2313067_-|WP_096429025.1|DBSCAN-SWA
MIKNKNTDHLKSLVSALPEEPGVYQFFDESNTIIYVGKAKRLKRRVASYFNKVHDSAKTNIMVRKIVDIRHIVVESEADALLLENNLIKKYQPRYNVLLKDDKTFPWICIKKEEFPRVLVTRYLLKDGAEYFGPYTSVRMVRVLMDMISSLYQLRTCNLKLSEEGVRDQKFKLCLEFHMGNCKAPCEGKENQEEYNLKIAEIRNILKGNIGTVTSHLKEKMQELSVDFKFEEAHIIKEKLDLLSKYQSRSTIVNPSINNVDVYSIVDDENFAYVNFIKVVNGAIIQAHTIEMKKKLDESKEDLLLLAITDIRQKIFSTAKEILVPIELDLVIENVRFIVPQRGDKKKLLDLSERNVKYYRLEKLKKGDKSIKQSHADRIVETMRTDLRLKDAPVHIECFDNSNIQGTIPVASCVVFRNARPYKKDYRHFNIKTVEGANDFASMEEIIYRRYRRLLDENKSLPQLIVIDGGKGQLGAALNSLDKLELRGKISVIGIAKKLEEIYFPGDPFPLYLDKNSETLKTIQHLRNESHRFAITFHRQKRSKAFIVSELDKIEGIGPKSVQKLIQKFKSVENVRKASVAELETELGNAKALTVHKYFN
>NZ_AP018042|2309575:2347311|2313093_2314737_-|WP_096433550.1|DBSCAN-SWA
MTSNTFSISGKVIDVISREIIKAEVVVEEGRITSVTQRDSVPDHYILPGLVDAHMHIESSLLIPSRFANLAVRQGTLGLVTDPHEIANVLGEIGVDFMINDAKKVPLEIRFGVPSCVPATPHETSGFVLDSNIVESLLKREEVVCLAEVMDFVGVINDDLEVLKKIVACKKLGKKIDGHAPGLKGQDLKKYVASGISADHECSTLEEALEKISLGMMIQIREGSAAKDFDALCSLLETHPEKIMLCTDDLHPDDLIEKGHINYLIKKGLKKGLDLFSLLRAASYNPIHHYGLDLGLLQVGHSADFIVIDNPESFIVEQAFRKGVCIYKDNEVLFDVSNEIILNNFSRKPIQLSDLIVKAENGSLRAMVVKDGDLITDSVLCKPKVVDKNVVSDVDNDLLKMVVMSRYDNGNPVMGFAKGFGYKKGAIAESIAHDSHNIIAVGTSDEELLHAINTLIEMKGGIVAVNNGESESVRLEVAGLMSNKTGEDLVREYAKLNDFASALGSNFESPFMTLAFMSLLVIPKLKLSDKGLFDVKEFKVINLFKEE
>NZ_AP018042|2309575:2347311|2339126_2339951_-|WP_162845418.1|DBSCAN-SWA
MNFSKKVFLAIVIILITKLTFGQATNSIILIETNMGNIKLMLYKETPKHRQNFLQLIENGHFDGTLFYRVIKGFVAQGGSQDSRNAPAGKMIGYGSSNRTIESEFNKKFFHKKGAIAAPRKPDNVNIFKESDISQFYIAHGRVFSDEELTLMEKEVNVPLRKRIVKRYLTLDKRAVLDSLKKADKVDEFRAIAKTIKGSIEFAYNSSNLKLEFSEARRKAYTTIGGVPHLDGNYTVFGEVVEGLDVLDKIVDLETDENDRPFQDVKMKVKILEY
>NZ_AP018042|2309575:2347311|2321071_2322298_-|WP_096429030.1|DBSCAN-SWA
MGISKITKGKIFYLVIGLLFLFSVDALSQNNISTLKKRKEQNAKDIAYTNTLLTNTLKNKNVSLGQLNLLNQRIKARQALIQSIENEISYMGSQISKTNADVKGLEEDLKKLKQQYAKVVQYAYKQRNSHEKLMFLLSSKDFNQAYKRYKYLQQFSEYTRKQGKMIAVKKDTLDATLLHLKAAKNEKVILLSEKTKENTSLAQEKSQQSEIVSKLKQRERQLRDDLNKQQRYTRKLENEIQKIIENQAKLASKKNNGGGKYGLTPEEKILSDSFDKNKGKLPWPTKTGFISEKFGQHKHAVLKHVNVRNDGVNITTDTKAECRSIFKGEVTHILSMPGLNNVIIIRHGEFFSVYSNLSTVAVKKGDTVTSKEKIGVVYTDQAQSQTVLKFQLWKGSTKLDPSLWLYRN
>NZ_AP018042|2309575:2347311|2346704_2346806_+|WP_145957604.1|bacteriocin|DBSCAN-SWA
MKNFEVVSQEELQNIKGGYNEMTDTCTLDDCIV
>NZ_AP018042|2309575:2347311|2347209_2347311_+|WP_145957605.1|bacteriocin|DBSCAN-SWA
MKNFEVVSQEELKNIKGGYNEMTDTCTLDDCIV
>NZ_AP018042|2309575:2347311|2343492_2346336_-|WP_096429050.1|DBSCAN-SWA
MKNFLKKILIYPLFPLFLLFLFSGSTNSKSNSDLEKHFPVEKVNPDSLLQVQFDRAVGFLKKGEFDFSIQSFENAILTINKGGLSDKNLIYRTYVNFGVLLNRVGDRKRAIEFYNEAEIFTIAEYSQNSNKLIPIYINKGNIYNDYEDLIKAENYYKKSLSLIDIVNGSKWESQLYHNLGVIYYKKKLYSEALTYLLKTLNIKTKQKTGNTSTTRNIIANCYKKLGNYEEADSYYQKSISEVKEINGFHFYLLGDYYLNYAVFQDQIGNSDKVLSYLNLAYDVYVKNFGGKHPDTAHCLKNFGDYYSKRNDHLNALKYYQKALIAETDNFNNSTVYINPSMDELDPQLSILEILKPKANTLQELYSKTNSIPDLDYSLQTYDLCLDIIDNIRIAYQDEESKFALSKNEKDTYTEAIEIAVELYQLTKDVKYKEKAFRYAERSKSASLLSSLNDVNAKNFGGISIELQEQERDLKLNIAKYREFVHDEKRKQTPNRDSISGWQNVLFNLNEEYNQMVLNFEEDYPEYYSLKYDTKTITIEDLQSRLDDNDVLIEYSISDSALFSFIISKSSFELERQKIVKDTFDYHLEEVRNCLKTNDFSNNSADYYKRYTRSAYQLYQYLLSGKDSLLSGKNLLIVPDDKMAYIPFGVLLTKEADSTTMNYRNLEYLIKENTITYHNSATLGFKNHSTSYGVFSSKSVLAFAPSYEGVNDSILFTERSYRDKLYPLPGVKEEVNNISNVIHGDLFVDDLATEANFKANASNYDVLHLAMHTIIDDENPMYSKLVFTQNSDTIEDGLLNTHEIYNMNFNARMVVLSACNTGDGKLLKGEGVMSLARGFFYAGCPSVIMTLWTVEDKTGSNLMSNFYTFLSQGLHKDDALRQAKLEYLKTADSLKSHPYFWSGYVTLGDVDPLYDDSLVNKLMYFAFGSVGIIFLLLFRKKLRRKSVA
>NZ_AP018042|2309575:2347311|2324937_2325942_-|WP_096429033.1|DBSCAN-SWA
MKIIVPMAGMGKRMRPHTLTVPKPLIPIAGKPIVQRLVEEIAKVSDEAIDEIAFIIGDFGKDVEDILRGIAENIGAKASIYHQEEALGTAHAIYCAAPSLSGKVVVAFADTLFKADFVLDAEADSVIWVQKVEDPSAFGVVKIDGKNNITDFIEKPKDFVSDLAIIGIYYFKDGDNLRDELKYLMDNEVVVNGEYQLTDALENMKNKGLSFKPGQVIEWMDCGNKDATIHTNQRILEYHKGDELVCSSSTAENSIIISPCYIGENVKIKNSVIGPHVSVGANTTVSNTLVRNSIIQRNSNLENLNLENSMIGNYVDIQGEVSEWNVGDFATFSK
>NZ_AP018042|2309575:2347311|2332281_2332647_-|WP_096429039.1|DBSCAN-SWA
MKPNPVNDKSYQFSVRIINLYKFLKSNKHEYVLSKQILRCGTSVGANIEEALGGISKKDFIAKLHISFKEARETHYWLRLLKDTEYIDDKMFESLILDCEEILKLLSKIIETSKSNLSQDK
>NZ_AP018042|2309575:2347311|2346872_2346974_+|WP_145957604.1|bacteriocin|DBSCAN-SWA
MKNFEVVSQEELQNIKGGYNEMTDTCTLDDCIV
>NZ_AP018042|2309575:2347311|2314899_2316777_-|WP_096429026.1|tRNA|DBSCAN-SWA
MLPKYDVIVVGAGHAGCEAAVAAANLGSSVLLITMDMNKIAQMSCNPAIGGIAKGQIVRELDALGGFTGIVADRSSIQFRMLNRSKGPAMWSPRSQCDRMIFSSEWRKIVENTDNLDMWQDAVVGILIEEGKAVGVTTKLGIDIPAKSVILTNGTFLNGLIHVGSKQEKGGRTSEPASVGISEQLKSVGFEVGRMKTGTPARLDGRSIDFSLMKRQDGEEGFYQFSYLTDHVDRTLTQRPCYITYTNPKVHDELRKGFEESPMYNGTIQSTGPRYCPSIESKLSTFAEKQEHLLFLEPEGEYTHEYYVNGFSSSLPWDVQLKALQQVPGLEKVKMYRPGYAIEYDYFDPTQLYHTLETKLVSNLFFAGQINGTTGYEEAAAQGMMAGINAGLSVQEKDAFTLNRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADLRLSPKAFELGLVGEKRMNLLKEKIEFRDKLVTFCKEFSCKPKFLNPVLAKLGTAPLKQGVKLYDIILRPQVQILDLVEDVLPLKELIDSIPSRREEVIEAAEVLIKYSGYINREKLIAEKLTRLENINIKGRFEYTSLQSISTEARQKLDKIQPQTIGQASRISGVSPSDINVLLVLMGR
>NZ_AP018042|2309575:2347311|2347041_2347143_+|WP_145957604.1|bacteriocin|DBSCAN-SWA
MKNFEVVSQEELQNIKGGYNEMTDTCTLDDCIV
>NZ_AP018042|2309575:2347311|2340904_2341267_-|WP_096429047.1|DBSCAN-SWA
MDNNLESDWNKILYKISEDFNVDADLNGTLLLIGIQERGKGFQEKYEKDEKMDHINLATCTLLMKWNYYQIDGYDENQWPVFKKNKMVPPFSKEKEELLLKSSIVEYFKENDYLETENKR
>NZ_AP018042|2309575:2347311|2328534_2328903_-|WP_096429035.1|DBSCAN-SWA
MSLIGNIIWIIFGGIFIFLEYIICGFLMCLTIVGIPFGLKIIQLSVLGLAPFGQKVEYNEHAGGCLSILLNVIWIVIGGIWIAITHLLFALVFAITIIGIPFAVQHVKLAGFALTPFGKSIR
>NZ_AP018042|2309575:2347311|2332688_2334896_-|WP_096433554.1|DBSCAN-SWA
MKKKFPHFRQLDAMDCGPTCLRMIAAHFGKNFSLQSLRDKSHITREGVSMMGIADAAESIGLRTMGVRISYEQLQNDAALPAICHWGQNHFVVVHKIEVKKKKTTVHVADPASGLLKFTKEEFIKSWASTQSQGELKGLCLLVETTPDFYAQGDEDLSHKKSFTFLLQYLRPYKKFLVQLVLGMLFGSMLQLIFPFLTQSIVDVGINTQNLSFIYLVLIAQLVLFISRMSVEFIRSWILLHISTRINISLISDFLIKLMKLPIGFFDIKMIGDLLQRIQDHTRIENFLTSSTLSILFSMVNLVIFGIVLAIYDMQIFFVFVVGSLLYIIWVNLFLRKRRELDGKRFAQLSNNQNSLVQLITGMQEIKLNNCEKQKRWEWEQIQAELFKVRTKGLALNQYQVSGSIFFNETKNIIITFLAAKSVLDGGMTLGMMLAVSYILGQLNSPLDNLVQFIHSWQDAKISLERLGEIHEMKEEEMPNEDRLTEIPEEKAIDIEKVFFQYEGPHSEMVLDDVNLKIEDGKTTAIVGASGSGKTTLIKLMLGFYPTTKGRISLGGVTLENYSSSMWRQNCGVVMQDGFIFSDTIAKNVAVGVENIDKKRLLYAVQVACIDMFIESLPLRYNTRIGANGHGLSQGQKQRILIARAVYKNPKFLFFDEATNALDANNEKAIMNNLDEFNKGRTVVVVAHRLSTVRSADKIIVLDKGKIVEEGTHENLTKLKGAYYQLVKNQLELGN
>NZ_AP018042|2309575:2347311|2340060_2340873_-|WP_096429046.1|DBSCAN-SWA
MKNLIYIFIVFLFISCQKKLELGKKDRVVEVETEYGNLKIRLYDETPVHRDNFVKLTHDKFFDGTLFHRVIDGFMIQGGDPDSKSAKSGERLGEGGPGYQIPAEIKNGLFHKRGVLAAAREGDDVNPEKKSAGSQFYIVQGKVYTVGELDTLQTKLNGKLRSSIFKKVQMEKAEFLSKCQMEGELEKLTEAIDHIKFEVDSMFNLEKIVLSKQQIEAYTTVGGIPHLDGNYTVFGEVIEGMDLIDSIARVEKDDFDRPLKDISMKIKILK
>NZ_AP018042|2309575:2347311|2335289_2335838_-|WP_096429041.1|transposase|DBSCAN-SWA
MKKFQNKYRIPSARAVWWNYSNEGSYFVTICSHNRQWFFGSITDQKMELSEIGKLAHQFWFGIPNHFPFVKLHAFVVMPNHVHGIIEISNIAEETLHATSVLDGSDSKNKKIAAISPKKGSLASILRSYKSAVTKEARKIDPDFEWQERFHDHIIRDEPEYHRIENYINNNPRKWDDDKFNE

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096429023.1|2309575_2310028_-|tRNA	D-tyrosyl-tRNA(Tyr) deacylase	NA	NA	NA	NA
WP_096429024.1|2310210_2311194_+	lipoate--protein ligase	NA	NA	NA	NA
WP_096429025.1|2311264_2313067_-	excinuclease ABC subunit UvrC	NA	NA	NA	NA
WP_096433550.1|2313093_2314737_-	adenine deaminase	NA	NA	NA	NA
WP_096429026.1|2314899_2316777_-|tRNA	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG	NA	NA	NA	NA
WP_096429027.1|2316928_2317354_-	rRNA maturation RNase YbeY	NA	NA	NA	NA
WP_096429028.1|2317356_2317764_-	ATP-binding protein	NA	NA	NA	NA
WP_096429029.1|2317756_2321050_-	hypothetical protein	NA	NA	NA	NA
WP_096429030.1|2321071_2322298_-	peptidoglycan DD-metalloendopeptidase family protein	NA	NA	NA	NA
WP_096429031.1|2322305_2323169_-	DUF4292 domain-containing protein	NA	NA	NA	NA
WP_096429032.1|2323158_2324931_-	tetratricopeptide repeat protein	NA	NA	NA	NA
WP_096429033.1|2324937_2325942_-	NTP transferase domain-containing protein	E3SJ89	Synechococcus_phage	1.6e-08	25.2
WP_096433552.1|2326045_2326480_-	dUTP diphosphatase	V9SFR6	Tunisvirus	1.5e-40	57.0
WP_096429034.1|2326669_2328163_-	polysaccharide biosynthesis protein	NA	NA	NA	NA
WP_096429035.1|2328534_2328903_-	YccF domain-containing protein	NA	NA	NA	NA
WP_096429036.1|2328923_2329916_-	DUF3187 family protein	NA	NA	NA	NA
WP_096429037.1|2329964_2330912_-	ROK family protein	NA	NA	NA	NA
WP_096429038.1|2330931_2332224_-	HlyD family efflux transporter periplasmic adaptor subunit	NA	NA	NA	NA
WP_096429039.1|2332281_2332647_-	four helix bundle protein	NA	NA	NA	NA
WP_096433554.1|2332688_2334896_-	peptidase domain-containing ABC transporter	W8CYL7	Bacillus_phage	3.2e-41	30.5
WP_145957602.1|2334986_2335187_-	hypothetical protein	NA	NA	NA	NA
WP_096429041.1|2335289_2335838_-|transposase	transposase	NA	NA	NA	NA
WP_096429042.1|2336168_2336873_-	Crp/Fnr family transcriptional regulator	NA	NA	NA	NA
WP_096429043.1|2337083_2337503_+	OsmC family protein	NA	NA	NA	NA
WP_096429044.1|2338004_2339027_-|tRNA	phenylalanine--tRNA ligase subunit alpha	A0A2K9L3A8	Tupanvirus	3.8e-29	32.5
WP_162845418.1|2339126_2339951_-	peptidylprolyl isomerase	NA	NA	NA	NA
WP_096429046.1|2340060_2340873_-	peptidylprolyl isomerase	A0A076FI46	Aureococcus_anophage	5.2e-05	30.8
WP_096429047.1|2340904_2341267_-	hypothetical protein	NA	NA	NA	NA
WP_096429048.1|2341911_2342478_+	sigma-70 family RNA polymerase sigma factor	NA	NA	NA	NA
WP_096429049.1|2342449_2343220_+	hypothetical protein	NA	NA	NA	NA
WP_096429050.1|2343492_2346336_-	CHAT domain-containing protein	NA	NA	NA	NA
WP_145957603.1|2346536_2346638_+|bacteriocin	bacteriocin	NA	NA	NA	NA
WP_145957604.1|2346704_2346806_+|bacteriocin	bacteriocin	NA	NA	NA	NA
WP_145957604.1|2346872_2346974_+|bacteriocin	bacteriocin	NA	NA	NA	NA
WP_145957604.1|2347041_2347143_+|bacteriocin	bacteriocin	NA	NA	NA	NA
WP_145957605.1|2347209_2347311_+|bacteriocin	bacteriocin	NA	NA	NA	NA

Sequences of DBSCAN-SWA_3

Nucleotide sequences of DBSCAN-SWA_3

>NZ_AP018042|3953764:3962878|DBSCAN-SWA
AATGTTAAAAAATCATGTAGCAATACTGATTAATGAAAGTTTGAACTTCGATCCAACCGCAGACCAGCACAGGGCAATTGACTGTTTGGCGGATTATGTAACCAATAATGACGAATGTGGAATTTTTTTGTTAAAAGGTTATGCCGGTACAGGAAAGACGACTTTGGTGAGTGCTTTGGTATCTGTTTTGGATAAAATGAAAATTAATTCAGTGCTTCTCGCTCCAACTGGACGAGCGGCAAAAGTTCTTACTTACTATTCAAAAAAATCAGCTTATACAATCCATAAAAAAATTTATCGTCAGAAATCTCTTACTGATGATCTCGGTTTGTTCGCTCTAGATCGAAATATGAACAATAATACTGTTTTTTTGGTGGATGAGGCATCAATGATTTCAAATTATTCTGCAGAGGCATCGGTTTTTGGTTCTGGCAGATTGCTGGATGATTTAATGCAATATGTATTCTCAAGGAAAAATTGTCGGTTGATTTTGATTGGGGATACGGCTCAGTTGCCTCCAATAGGATTGGATATTAGTCCTGCTCTTGATTCTCATGAACTGGAGTCATGCTACAATATGCCGGTAAAGGAGATGATCTTAACTCAGGTTGTTCGTCAAAATCAAGAATCGGGTATTTTAATGAACGCAACGAACTTAAGATGCATGCTGGCCGATGAAAAAAAAGGCTATCCTGTTTTGTTTACAAAAAATTATCCTGATATAAAACGAATTGGAGGAGCCGAATTGATTGAGGAAATTTCGAACTCCCATTCTGAGTTTGGTCTTGAGGATACTATTGTAATTAGTCGTTCTAATAAAAGGGCAAATAAATTCAATCAGGGAATTCGAAATGCGGTATTGTATCGCGAGGAAGAGATTTCCAGTGGAGATTATTTGATGGTTGTGAAGAATAATTATTTCTGGGCACAGGATATAAAGGAAATGGATTTTATAGCCAATGGCGATATTGTAGAAATCGTACGAATACATAAGTATATGGAAATGTATGGATTTCGTTATGCTGAGGTCACTATTCGGTTTCCGGATTACAAGGATATTGAATTAGATACCATGATCATGTTAGACACACTTGATATTGAAACGGCATCTCTTGGATACGAGAAAAATAAAAAACTATTTTTTACCATTGCCGAAGATTATGCGGATATTCGTTCTAAGAAAGCGCGATACGAAAAAGTGCGGGACAATAAGTTTTTTAATGCTTTGCAAGTGAAATTTTCATATGCTGTTACCTGTCACAAAGCTCAGGGAGGACAATGGAAATCAGTATTTGTAGATCAAGGATACTTAATCGATGAGTTGTTAACGCCAGAGTATTACCGTTGGCTCTACACGGCGGTAACTCGTGCTACCGAAAAATTGTTTCTTGTTAATTTTAAAAAAGAATTCTTTCCTGATGAGAATGAGATTTAAAGCTCAATGCCAATAATCAGAATGTCGTCGGTTTGTTCATTGTTTTCTTTCCAACTTTCAAACCAGTTGAAGATATCTGATTTCTGCAGGTTGATGGATTTGTTTTGAATGGATAGTAATTTGTCGTAAAATTGCTTCTTTTTAAGTGTTTTAAGCTGTTGTCCGCCAAGTTGGTCTGTAATCCCATCTGTGTACAAGAAAATCCTGTCACCAGCCTGTGTTGCTATCTTTTTAGTTGTGAATTCAGCAAATTCGGATTCTTTACCAATTGAATTTCGATCACCTTGAATTTCGAAAAGAGAATAGTCCTCATTACTAACTTTTGCTTCAGTCAATTCATTGTCGCATTTTACAGGCTCTGAACTTTTCCTGATCAAAACCAGTGATCTTTTTGCTCCTACAAATTCCAATATCTGGTCTTGTGGGTAAATGGAGCAAAATAACCCATCCATGCCGTCGTACTGAGTATAAGTATCGCCAGATGTGTTCAGAATTCGACTAAATTTTTGACTTAACAGAGGAAAAACATGTTCAGCACTAACTTCGTTTCCATTTTGAGAAAGAGTATGCAGTTCCATTTGTCCCATAACTGTTAACATCGCAGCAGGAACTCCGTGTCCGGTGCAATCAAATAAGCCAATGTTTTTTTTATGGAAATTCTGTTGAATCCAATAAAAATCTCCACTCACAACATGTTTGGGCAGATTAATAATAAAGGATTTGGAGAATAATCTTCTGAAATTTTCAGCATCAGGAAGAATAGATCTTTGAATTCTACCTGCATGAATCATACCTTGATGAATGTCATAGTATGACTTGTATTTTGAAATTTCTTGTTTTTGTTCATTGATTTGAAATGCAATTAATACTTTAGTTTGCAGTTCCGTAAAATCAATTGGCTTTTTAACGTAATCTTGTGCACCTAAACTTAAGGTGGCTTTTATGGACTCCATTTCTGTTTTGGCCGAAACCATTATGACAGGAATGTTTTTTAATCTTTCATTCTCCTGCATTTTTTCGAGCACCTCAATGCCATCCATTACCGGCATCATAATGTCGAGAAGAATAACATCTGGAACGTTGTCGTTTAACCAGTCGAGAGCTTGCTGACCACCCTCCTTAGAAATGACAGTATAATCGCGTCTGAAATAAGCTTCAATTAATTTATTATTAATTGGAGAGTCGTCAACAGATAGAATGGTTCTTTTGTTATCCATAATGAGTTAAAAGATATAGGGCAGCATAAAGCTACCCTAAAAATATTAGTTTAATTTCAAAGAGCTAATAATTCCTTGAATTTTTTTGTTGGCTTTTTCTTCAGTAGCGTAAAAATCTTCAATTGAAGCAAGTGTTTCGCGCACCTCGAAATAGTATTTTATTTTTGGCTCAGTTCCCGATGGACGAACAGAAATCTTTGTTCCGTCTTCCAAATAAAATTGAAGAACATCAGCAGTAGTCTCAAAATTCAATGCCGTTTCTTCACCAGTTACTGGGTTTGTAGCTATTCTTGTTGTGTAATCCTTCACCAAAACTAAGGTCGATCCATTAATTTCTTTCGGACGATTGTAGCGATAGTCGTACATTAATTTTTCAATTTCCTGTGCGCCTTGCAGTCCTTCACGAACGATATAAATCATTTTCTCTTTCGAATAACCATATTCTACATAGATATCTTTAAGAATATCGAATACGTTTTTCCCTTGCTCTTTAGCCCAGGCTGCAATCTCGGCCATAATGCTACAAGAAGATACTGCATCTTTATCACGGGTAAAATCACCAGGCATATAGCCAAAAGATTCCTCGCCACCACCGATATAGTTTTTCTCAGGATTTTTTCTGATTACATAAGCAATCCATTTAAATCCTGTGTAAGCATCAAAACATTGTACATTGTTTTTCTCTGCTACCTCTTTGAATAACTCTGTTGTAACAATAGTTTTTACCACATAATCGTTACCAGTTAAAAGTCCAAGTTCTTTTCTTCTTCTAATTAAGTAGTAGTTAAAAATAAGAGCAGTCTGATTTCCATTTACTATTTCCCATTCTCCTTTATCGTTCGTAGCAGCAATGCCCAAGCGGTCACCATCAGGGTCACAAGCCATTACTATATCAGCATTAACTTCCTTGGCTTTATCAACTGCCATTTTTAACGCTTCTGGTTCTTCAGGATTTGCCGACCAAACAGTAGGGAAGTTACCATCTACTACATCTTGTTCTGGAATATGAATTACGTTCTCAAAACCGTAATTTTTTAGTGAAGCAGGAACCAATCTTACTGTTGTTCCATGTAATGGCGTAAAAACAATTTTTAAATCTTTTTGATTTTTGATTGCTTCAGGAGAAAGACTTAATGTTTTAACTTTATCAAGAAAAAGTTTATCCATATCTTCGCCAAGAACTTCGATTAGAGCAGGATTCCCTTTGAATTTGATTTCTTCAAGTTTTACTGCCTTAACTTCGTCAATTACATTTTTATCGTGAGGAATAACCAATTGAGAACCATCTTCCCAATATGCTTTATATCCATTGTATTCTTTAGGATTGTGTGATGCAGTAATAATAACACCTGATTGACAACCCAAATGACGAATTGCAAAAGACATTTCTGGTGTTGGTCTTAAATCTTCAAAAATATATGCTTTAATACCATTTGCCGAGAAAATATTAGCAACAGTATCCGAGTATAATTTGCTATTGTTTCTACAATCATAGCCAATACAAACAGAAAGATTTTCTTGTCCTGCGAAGGCTTTATTAATATAGTTGGCTAATCCTTGAGTAGCGGCACCAACAGTGTAAATATTCATTCTGTTAGTTCCTGGACCCATTTTACCACGTAAGCCTCCAGTTCCAAATTCAAGATCTTTATAAAATGATTCTATTAAATCAGTTTTGTCTTCCTTTTCTAATAATGATTTTACAGCATTTTTAGTTTCCTGATCATATTTCCCCTCAAGCCATGCTTGGGCTTTTTCATTTACGATTTTAAAAATCTCAGTCTCGTTCATCTTTGATACAATTTCTTTCGGTTAACTATTCAGTAATTCTTTTAATACAATGTTCAATTCTGCTTCTCCAGTGTGGTATTTCGAGTGTAAAAATATGTTTAATTTTCGATTTATTCAATACGCTGTAGTGTGGTCTTTTTGCAAGGCATTGAAAATCTTTGCTTTCGATGCTGTTAATTTTACATTTATTTTTCGATGTGTTTTGTATTTCAGTGGCGAAATCGTACCAACTACATACGCCTTCGTTGGAATAATGATAGGTTCCAAAATCAGTAAAGTGACTCTTTTCAGTGATGCTTTGAATGATATGCAAAATGGTGTAAGCCAGGTCGTGCGCATAGGTGGGAGAGCCAATTTGGTCGCCTACAACATCTAGTGAATCTTTTTCTTTGCCTAAGCCTAGCATTGTCTTAAGAAAATTTTTCCCAAATGGAGAGTATAACCAAGAGGTTCGTATGATAATATGTTGTTGGCAAATGTTTCGAACATTTTCTTCACCTTCAAATTTGGTTCTGCCGTAAACACCCATGGGTTTAGCCTTCTGATCCTCAGTGTATGGTGTTGAATTATCTCCAGAAAACACATAGTCGGTAGATATGTGAACTAAAATAATATTGTTCTGCATGGCTATCGTAGCCAGATTTTTTACCGCATCAGCATTGATTAATTTGGCTGCTTGCTCATTTTCTTCCGCCTGATCAACATTAGTGTATGCTGCGCAATTTACGATGTAGGAAATTTGTTTGTCGGTAATGTAACTGTCTATTGCATCCAAAGCACAGATATCCAGTTCCTCAATATCCGTAAAGAAAAATTGAAGATTATTAAACTGAATCGATAACTCTTTTATTTCGGAGCCTAACTGTCCTTTAGAGCCTGTAATTAGGATGTTTGTCATGCTTTATTTACTGTATTTAAAATTTTTCTCCACCTCTTTAAAGTTTGGAAACACTTTGTCCTTTGGGGAGATAATGGCTTTGTTAGGCTCTACTCTCCAATTAATATTTAAGAATGGATCGTTAAAATTGATCCCTCTTTCGGCTTTCGGATTATACAAGTTGTCACATTTGTAGATGAAGGTTGCAGATTCGCTTAAGACAGAAAAGCCATGAGCAAATCCTTTAGGGATCAGAAACTGAATTTTGTTATCCGCCGACAATTCAATACCACTCCATTGCCCGAAAGTAGGAGAGTCTTTTCTTAGATCAACAGCAACATCGAAAACTTTACCTTCAATTACCCGAACTAATTTCGTTTGTGAGTAAGGTGCCAATTGATAGTGAAGTCCTCTTATTACTCCATATCCCGATTTGGATTCATTATCCTGAACAAATGACAGATCTAGACCTTTTTCGCGGAAAATCTTTTCATTGTAACTCTCGAAAAAATATCCTCGTTCATCTTCAAAAACTTTGGGTTTGATTATTAATAAGTCTGGGATTTTTGTTTTAATAATTTCCATTTCGAATTCCCTTGTTTTTAATATTTTCATTGGCCACCATCATCAAATATTCCCCGTATTGATTTTTAAGTAATGGTTGCGCTAATTCAATTAAGTCTTCTCTGCTTATAAATCCATTACGATAAGCAATCTCTTCCAAACAGGCAACTTTTAATCCTTGCCTTTGTTCTATTGTTGCAATGTAATTAGATGCCTGAAGCATACTGTCGTGAGTTCCTGTATCCAGCCAGGCAATTCCGCGTCCTAAAACCTGAAGTTTTAGATTGTCTTCTTTCAGGTAAATTAGGTTTAGATCTGTTATTTCGAGTTCGCCTCGTTTCGATGGTTTTAATGATTTGGCCTTTTCAATCACAGTGTTGTCGTAAAAATACAAGCCAGTTACTGCGTAGTTGGATTTAGGATGTTCTGGTTTTTCTTCCAAGCTTTTGACTTTGCCATTTTCATCAAAATCAGCAACACCATATCTTTCCGGATCTTTAACATGATATCCAAAAACCAGTGCTCCTTCGGTTAATTTCGCCGAAGCTACCAGTTGCTTGCCAAATTCATATCCGTAGTAAAGATTGTCACCTAAAATCATACAAACTGGGCTGTCTCCAATAAACTCTTCACCAATAATAAATGCTTGTGCTAATCCGTCTGGCGAGGGTTGTTCGGCGTACGAAAGGTTTAGACCAAACTGGTTTCCATTTCCAAAAAGATCTTTGTAAAGATGTAAATCTTTTGGTGTTGAAATGATTAGGATTTCTTTTATTCCTGCAAGCATCAAAACAGAAAGAGGGTAGTATATCATTGGTTTGTCGTAAACCGGGATGATTTGTTTCGAAATGCTTTTTGTGATCGGATAAAGTCTTGTGCCCGAACCCCCAGCTAATATAATTCCTTTCATTTAGTGTGTCGGTTTGATTGATAATAAGTTATCAAAATACGAAATTGTTCGAAGTAAACCTTCATTTAATTGAATTTTTGGTTCCCAGTCGTTTAATTCCATTTTCGCAAGGCTAATATTCGGCTGTCTTTGTGTTGGATCGTCTTTAGGTAAAGGCAAATGAATGATTTTAGAGTTTGAATTGGTTAGTTCAATTACTTTTTGAGCCAATTCAAGAATTGTAAATTCATTAGGGTTGCCAACATTAACCGGACCAATAAATTCCTTTCTTGAGTTCATCATTCGTATCATACCTTCCAGTAGATCGTCAACATACTGAAAACTTCTGGTTTGAAGACCATCGCCAAAAAGAGTAATGTCCTCACCTTTTAGTGCCTGAACAATAAAATTCGATACCACACGACCATCATTTGGATGCATGTTAGGACCGTAGGTGTTGAAAATACGGACAATTTTTATGGCAACATTATTTTGGGTGTGATAATCCATGAACAAAGTCTCGGCACAACGTTTCCCTTCATCGTAACAGGAACGAATTCCAATTGGATTCACATTTCCCCAGTAGTCTTCTGTTTGTGGGTGTATTTCAGGATCACCATAGACTTCACTTGTTGAGGCCTGAAGAATTCGGGCATTTATTCTTTTGGCTAAACCAAGCAGGTTGATTGAGCCCATTACTGAAGTTTTGGTGGTCTTAATTGGATTGTATTGGTAGTGCACAGGAGATGCTGGACATGCCAAATTGTAAATTTGATCGACCTCGGCATAGTACGGTTGCGTAATGTCGTGACGAACAAGTTCAAAATATGGATTATCCATTAAATGAGCAATGTTCTTCTTTGAACCCGTGAAATAATTGTCCAAACAGATCACATCATTGCCTTCTTTTAGTAATCTCTCGCAAAGATGTGAACCAATAAATCCAGCTCCTCCGGTAATTAAAATTCTTTTCATAATTTAAATCCTTGATCCAGTTTTGGTTACCCAATAGATTCTTTTTTAACAATCTCCCGGCCAATGGAATAGTATGTGTAGCCCAGTTCTTTCATTTCGTAAAGATCATAGATGTTTCTTCCGTCGAAAATTAATTTCTCCCTCATTAATTTCTCCATCACTTTAAAATTTGGAAGTTTGAATTCCGACCATTCTGTAACAATTAGTAAAGCGTCAGCATCAATTAGCGCTTCGTATTGGTCTTTAGCATAGCTAATTGTATCGCCAATTTTTCGATGGCATTCTTCTGCTGCAACAGGATCGTAAGCAATTACTTCGGCACCTTCACTTAAAAGTTTGTTGATGATAACCAGTGATGGAGCTTCTCGCATATCATCAGTGTTTGGTTTGAATGATAATCCCCAAATAGCAATTTTTTTGCCTTTCAGATTTCCATCGAAATGTGTCATTATTTTTTTGTAAAGCACACTTTTTTGGCGGTTGTTTACTAATTCTACAGATTTTAAGATTTCAAGAGGATGCCCCCTTTGTTCTGACGTTTTGATAAGTGCCTGTACGTCTTTTGGGAAGCATGATCCACCATATCCAGTGCCTGGGTAAATAAATTTATTTCCGATACGGCTGTCTGATCCAATACCACGGCGAACATTGTTCACATCAGCTCCTACAATCTCGCAAAGATTTGCTATGTCGTTCATGAAAGAGATTTTTGTTGCCAGCATTGAATTCGCCGCATATTTTGTCATTTCGGCAGAAGGGATATCCATGAAAATTACCGGATGATTATTCATTAGAAATGGTTTGTAAAGCTTACTCATTACTTCCTGAGCTCTTTGCGACTCTGTTCCAATTACAACCCTGTCAGGTTTCAGAAAATCATCAATTGCGGCTCCTTCTTTCAAAAATTCCGGATTTGAAGCAACATCAAATTGAATGTCTTCGTTTCGGTTGGTAAGTTCTTCGTTAATAGTGGCTTTAACTTTTTCTGCCGTTCCAATTGGTACTGTGCTTTTGGTAACCACAACAAGGTAGCGGTCAATATATCGTCCTATTTCCTTGGCTACAGCAATTACGTATTGAAGATCTGCGCTCCCATCTTCATCAGGAGGAGTGCCAACAGCAATAAAAACGGCATCGGCATCTAAGATGCTATCTTTTAAGCTATTCGAAAAAAACAAACGGCCTTTTTCCATATTGCGTTTCACCATTGGCTCTAAACCAGGTTCGTAAATTGGAAGTATACCTTTATTTAAATTGTCAATTTTATTTTGATCTACATCAACGCAGGTAACAGTAATTCCCGTTTCTGCAAAGCAAGTTCCGGAAACTAAACCTACATAACCGGTACCAACAATAGTAATGTTCAT

Protein sequences of DBSCAN-SWA_3

>NZ_AP018042|3953764:3962878|3955194_3956415_-|WP_096430679.1|DBSCAN-SWA
MDNKRTILSVDDSPINNKLIEAYFRRDYTVISKEGGQQALDWLNDNVPDVILLDIMMPVMDGIEVLEKMQENERLKNIPVIMVSAKTEMESIKATLSLGAQDYVKKPIDFTELQTKVLIAFQINEQKQEISKYKSYYDIHQGMIHAGRIQRSILPDAENFRRLFSKSFIINLPKHVVSGDFYWIQQNFHKKNIGLFDCTGHGVPAAMLTVMGQMELHTLSQNGNEVSAEHVFPLLSQKFSRILNTSGDTYTQYDGMDGLFCSIYPQDQILEFVGAKRSLVLIRKSSEPVKCDNELTEAKVSNEDYSLFEIQGDRNSIGKESEFAEFTTKKIATQAGDRIFLYTDGITDQLGGQQLKTLKKKQFYDKLLSIQNKSINLQKSDIFNWFESWKENNEQTDDILIIGIEL
>NZ_AP018042|3953764:3962878|3960557_3961511_-|WP_096430687.1|DBSCAN-SWA
MKRILITGGAGFIGSHLCERLLKEGNDVICLDNYFTGSKKNIAHLMDNPYFELVRHDITQPYYAEVDQIYNLACPASPVHYQYNPIKTTKTSVMGSINLLGLAKRINARILQASTSEVYGDPEIHPQTEDYWGNVNPIGIRSCYDEGKRCAETLFMDYHTQNNVAIKIVRIFNTYGPNMHPNDGRVVSNFIVQALKGEDITLFGDGLQTRSFQYVDDLLEGMIRMMNSRKEFIGPVNVGNPNEFTILELAQKVIELTNSNSKIIHLPLPKDDPTQRQPNISLAKMELNDWEPKIQLNEGLLRTISYFDNLLSIKPTH
>NZ_AP018042|3953764:3962878|3958231_3959104_-|WP_096430683.1|DBSCAN-SWA
MTNILITGSKGQLGSEIKELSIQFNNLQFFFTDIEELDICALDAIDSYITDKQISYIVNCAAYTNVDQAEENEQAAKLINADAVKNLATIAMQNNIILVHISTDYVFSGDNSTPYTEDQKAKPMGVYGRTKFEGEENVRNICQQHIIIRTSWLYSPFGKNFLKTMLGLGKEKDSLDVVGDQIGSPTYAHDLAYTILHIIQSITEKSHFTDFGTYHYSNEGVCSWYDFATEIQNTSKNKCKINSIESKDFQCLAKRPHYSVLNKSKIKHIFTLEIPHWRSRIEHCIKRITE
>NZ_AP018042|3953764:3962878|3953764_3955198_+|WP_096430677.1|DBSCAN-SWA
MLKNHVAILINESLNFDPTADQHRAIDCLADYVTNNDECGIFLLKGYAGTGKTTLVSALVSVLDKMKINSVLLAPTGRAAKVLTYYSKKSAYTIHKKIYRQKSLTDDLGLFALDRNMNNNTVFLVDEASMISNYSAEASVFGSGRLLDDLMQYVFSRKNCRLILIGDTAQLPPIGLDISPALDSHELESCYNMPVKEMILTQVVRQNQESGILMNATNLRCMLADEKKGYPVLFTKNYPDIKRIGGAELIEEISNSHSEFGLEDTIVISRSNKRANKFNQGIRNAVLYREEEISSGDYLMVVKNNYFWAQDIKEMDFIANGDIVEIVRIHKYMEMYGFRYAEVTIRFPDYKDIELDTMIMLDTLDIETASLGYEKNKKLFFTIAEDYADIRSKKARYEKVRDNKFFNALQVKFSYAVTCHKAQGGQWKSVFVDQGYLIDELLTPEYYRWLYTAVTRATEKLFLVNFKKEFFPDENEI
>NZ_AP018042|3953764:3962878|3961537_3962878_-|WP_096430689.1|DBSCAN-SWA
MNITIVGTGYVGLVSGTCFAETGITVTCVDVDQNKIDNLNKGILPIYEPGLEPMVKRNMEKGRLFFSNSLKDSILDADAVFIAVGTPPDEDGSADLQYVIAVAKEIGRYIDRYLVVVTKSTVPIGTAEKVKATINEELTNRNEDIQFDVASNPEFLKEGAAIDDFLKPDRVVIGTESQRAQEVMSKLYKPFLMNNHPVIFMDIPSAEMTKYAANSMLATKISFMNDIANLCEIVGADVNNVRRGIGSDSRIGNKFIYPGTGYGGSCFPKDVQALIKTSEQRGHPLEILKSVELVNNRQKSVLYKKIMTHFDGNLKGKKIAIWGLSFKPNTDDMREAPSLVIINKLLSEGAEVIAYDPVAAEECHRKIGDTISYAKDQYEALIDADALLIVTEWSEFKLPNFKVMEKLMREKLIFDGRNIYDLYEMKELGYTYYSIGREIVKKESIG
>NZ_AP018042|3953764:3962878|3959654_3960557_-|WP_096430685.1|DBSCAN-SWA
MKGIILAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSVLMLAGIKEILIISTPKDLHLYKDLFGNGNQFGLNLSYAEQPSPDGLAQAFIIGEEFIGDSPVCMILGDNLYYGYEFGKQLVASAKLTEGALVFGYHVKDPERYGVADFDENGKVKSLEEKPEHPKSNYAVTGLYFYDNTVIEKAKSLKPSKRGELEITDLNLIYLKEDNLKLQVLGRGIAWLDTGTHDSMLQASNYIATIEQRQGLKVACLEEIAYRNGFISREDLIELAQPLLKNQYGEYLMMVANENIKNKGIRNGNY
>NZ_AP018042|3953764:3962878|3956460_3958206_-|WP_096430681.1|DBSCAN-SWA
MNETEIFKIVNEKAQAWLEGKYDQETKNAVKSLLEKEDKTDLIESFYKDLEFGTGGLRGKMGPGTNRMNIYTVGAATQGLANYINKAFAGQENLSVCIGYDCRNNSKLYSDTVANIFSANGIKAYIFEDLRPTPEMSFAIRHLGCQSGVIITASHNPKEYNGYKAYWEDGSQLVIPHDKNVIDEVKAVKLEEIKFKGNPALIEVLGEDMDKLFLDKVKTLSLSPEAIKNQKDLKIVFTPLHGTTVRLVPASLKNYGFENVIHIPEQDVVDGNFPTVWSANPEEPEALKMAVDKAKEVNADIVMACDPDGDRLGIAATNDKGEWEIVNGNQTALIFNYYLIRRRKELGLLTGNDYVVKTIVTTELFKEVAEKNNVQCFDAYTGFKWIAYVIRKNPEKNYIGGGEESFGYMPGDFTRDKDAVSSCSIMAEIAAWAKEQGKNVFDILKDIYVEYGYSKEKMIYIVREGLQGAQEIEKLMYDYRYNRPKEINGSTLVLVKDYTTRIATNPVTGEETALNFETTADVLQFYLEDGTKISVRPSGTEPKIKYYFEVRETLASIEDFYATEEKANKKIQGIISSLKLN
>NZ_AP018042|3953764:3962878|3959107_3959668_-|WP_096433687.1|DBSCAN-SWA
MEIIKTKIPDLLIIKPKVFEDERGYFFESYNEKIFREKGLDLSFVQDNESKSGYGVIRGLHYQLAPYSQTKLVRVIEGKVFDVAVDLRKDSPTFGQWSGIELSADNKIQFLIPKGFAHGFSVLSESATFIYKCDNLYNPKAERGINFNDPFLNINWRVEPNKAIISPKDKVFPNFKEVEKNFKYSK

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096430677.1|3953764_3955198_+	AAA family ATPase	A0A0H3UZA5	Geobacillus_virus	2.6e-15	26.9
WP_096430679.1|3955194_3956415_-	response regulator	W8CYM9	Bacillus_phage	3.4e-08	35.7
WP_096430681.1|3956460_3958206_-	phospho-sugar mutase	A0A1X9I671	Streptococcus_phage	3.7e-109	38.9
WP_096430683.1|3958231_3959104_-	dTDP-4-dehydrorhamnose reductase	A0A1D7XFA3	Escherichia_phage	9.7e-34	35.1
WP_096433687.1|3959107_3959668_-	dTDP-4-dehydrorhamnose 3,5-epimerase	I7HJC4	Enterobacteria_phage	1.4e-41	49.7
WP_096430685.1|3959654_3960557_-	glucose-1-phosphate thymidylyltransferase RfbA	I7I009	Enterobacteria_phage	9.5e-101	60.8
WP_096430687.1|3960557_3961511_-	SDR family oxidoreductase	A0A2K9KZK0	Tupanvirus	6.4e-79	47.6
WP_096430689.1|3961537_3962878_-	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	2.3e-90	42.8