CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP016629	Listeria monocytogenes strain FORC_049 chromosome, complete genome	1 crisprs	DinG,cas3,WYL,casR,DEDDh,csa3	1	0	7	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP016629_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP016629_1

521283-521867

Orphan

Consensus_repeat	Method
GATGTAAGTAATTTTGATACNAGTTCAGTAACTNATATG	CRT

7 spacers

The CRISPR arrays of NZ_CP016629_1

>merge|NZ_CP016629|1|521283-521867|CRT
GATTTGAGTGGTCTTGATACTAGTGCGGTAACTACTATGTGGGATATGTTTAACAGCTGTAGGGCACTTGAGGAACTGGATGTAAGTCATTTTGATACGAGTTCAGTAACTAATATGTCTTACATGTTTTATGATAATAGAAACCTTGAGGTGCTGGATGTAAGCAACCTTGATACCGGTTCAGTAACTAATATGTATGCCATGTTTGAAGATTGTACTAGTCTTGAAGAGCTGGATGTAAGTAATTTTGATACGAGTTCAGTAACTGATATGTATAGAGTGTTTAATGGCTGTGAAAAACTGAAGAAACTAGATGTAAGTAATTTCGATACCAGTTCCGCTACTGCAATGGTTCAAATGTTTAGAAATTGTAGCGCCCTTGAGAAGTTGGATGTAAGTAATTTTAATACGAGCTTAGTAACTGATATGCGTGCCATGTTTGCTGGTTGTACTAGTCTTGAAGCGTTAGATGTAAGTAATTTTGATACGAGTTCAGTAACTAATATGGCTGCCATGTTTTCTGATAATGAAAAACTGGAGAAGCTTGATTTAAGTACCTTTGATACCAGTTCAGTGACTAACATG

>NZ_CP016629|1|1|521283-521867|CRT
GATTTGAGTGGTCTTGATACTAGTGCGGTAACTACTATG	TGGGATATGTTTAACAGCTGTAGGGCACTTGAGGAACTG
GATGTAAGTCATTTTGATACGAGTTCAGTAACTAATATG	TCTTACATGTTTTATGATAATAGAAACCTTGAGGTGCTG
GATGTAAGCAACCTTGATACCGGTTCAGTAACTAATATG	TATGCCATGTTTGAAGATTGTACTAGTCTTGAAGAGCTG
GATGTAAGTAATTTTGATACGAGTTCAGTAACTGATATG	TATAGAGTGTTTAATGGCTGTGAAAAACTGAAGAAACTA
GATGTAAGTAATTTCGATACCAGTTCCGCTACTGCAATG	GTTCAAATGTTTAGAAATTGTAGCGCCCTTGAGAAGTTG
GATGTAAGTAATTTTAATACGAGCTTAGTAACTGATATG	CGTGCCATGTTTGCTGGTTGTACTAGTCTTGAAGCGTTA
GATGTAAGTAATTTTGATACGAGTTCAGTAACTAATATG	GCTGCCATGTTTTCTGATAATGAAAAACTGGAGAAGCTT
GATTTAAGTACCTTTGATACCAGTTCAGTGACTAACATG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP016629.1\|WP_061391596.1\|516681_518220_+\|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl\|CDD\|368260
NZ_CP016629.1\|WP_003728092.1\|514496_516047_+\|hydantoinase/oxoprolinase-family-protein	unknown	unknown	gnl\|CDD\|223223
NZ_CP016629.1\|WP_003734774.1\|526825_527068_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP016629.1\|WP_003725186.1\|505978_508138_-\|transglutaminase	unknown	unknown	gnl\|CDD\|376628
NZ_CP016629.1\|WP_003740428.1\|510390_511986_+\|PucR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|225306
NZ_CP016629.1\|WP_003734775.1\|527150_528128_-\|HD-domain-containing-protein	unknown	unknown	gnl\|CDD\|224004
NZ_CP016629.1\|WP_031644083.1\|508134_509283_-\|DUF58-domain-containing-protein	unknown	unknown	gnl\|CDD\|224635
NZ_CP016629.1\|WP_003727314.1\|529744_530104_+\|DUF3139-domain-containing-protein	unknown	unknown	gnl\|CDD\|371476
NZ_CP016629.1\|WP_003728096.1\|505106_505862_-\|DUF4866-domain-containing-protein	unknown	unknown	gnl\|CDD\|379782
NZ_CP016629.1\|WP_003734772.1\|525147_525765_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP016629.1\|WP_003728093.1\|513403_514504_+\|DUF917-domain-containing-protein	unknown	unknown	gnl\|CDD\|226065
NZ_CP016629.1\|WP_003728455.1\|522760_523234_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP016629.1\|WP_003721253.1\|523253_523739_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP016629.1\|WP_003721242.1\|509287_510235_-\|MoxR-family-ATPase	unknown	unknown	gnl\|CDD\|223786
NZ_CP016629.1\|WP_003728992.1\|512119_513403_+\|cytosine-permease	unknown	unknown	gnl\|CDD\|271377
NZ_CP016629.1\|WP_003727318.1\|523766_524072_+\|LPXTG-cell-wall-anchor-domain-containing-protein	unknown	unknown	gnl\|CDD\|273478
NZ_CP016629.1\|WP_012681110.1\|528889_529717_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|368573
NZ_CP016629.1\|WP_089179989.1\|518483_520490_+\|BspA-family-leucine-rich-repeat-surface-protein	unknown	unknown	gnl\|CDD\|308794
NZ_CP016629.1\|WP_003734773.1\|526074_526422_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP016629.1\|WP_014929391.1\|524147_524414_+\|transposase	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP016629.1\|WP_061391596.1\|516681_518220_+\|helix-turn-helix-domain-containing-protein	gnl\|CDD\|368260	pfam05043, Mga, Mga helix-turn-helix domain. M regulator protein trans-acting positive regulator (Mga) is a DNA-binding protein that activates the expression of several important virulence genes in group A streptococcus in response to changing environmental conditions. This domain is found in the centre of the Mga proteins. This family also contains a number of bacterial RofA transcriptional regulators that seem to be largely restricted to streptococci. These proteins have been shown to regulate the expression of important bacterial adhesins. This is presumably a DNA-binding domain.	9.34931e-20
NZ_CP016629.1\|WP_003728092.1\|514496_516047_+\|hydantoinase/oxoprolinase-family-protein	gnl\|CDD\|223223	COG0145, HyuA, N-methylhydantoinase A/acetone carboxylase, beta subunit [Amino acid transport and metabolism / Secondary metabolites biosynthesis, transport, and catabolism].	1.15698e-106
NZ_CP016629.1\|WP_003725186.1\|505978_508138_-\|transglutaminase	gnl\|CDD\|376628	pfam01841, Transglut_core, Transglutaminase-like superfamily. This family includes animal transglutaminases and other bacterial proteins of unknown function. Sequence conservation in this superfamily primarily involves three motifs that centre around conserved cysteine, histidine, and aspartate residues that form the catalytic triad in the structurally characterized transglutaminase, the human blood clotting factor XIIIa'. On the basis of the experimentally demonstrated activity of the Methanobacterium phage pseudomurein endoisopeptidase, it is proposed that many, if not all, microbial homologs of the transglutaminases are proteases and that the eukaryotic transglutaminases have evolved from an ancestral protease.	4.40206e-28
NZ_CP016629.1\|WP_003740428.1\|510390_511986_+\|PucR-family-transcriptional-regulator	gnl\|CDD\|225306	COG2508, COG2508, Regulator of polyketide synthase expression [Signal transduction mechanisms / Secondary metabolites biosynthesis, transport, and catabolism].	2.42853e-59
NZ_CP016629.1\|WP_003734775.1\|527150_528128_-\|HD-domain-containing-protein	gnl\|CDD\|224004	COG1078, COG1078, HD superfamily phosphohydrolases [General function prediction only].	3.2717e-43
NZ_CP016629.1\|WP_031644083.1\|508134_509283_-\|DUF58-domain-containing-protein	gnl\|CDD\|224635	COG1721, COG1721, Uncharacterized conserved protein (some members contain a von Willebrand factor type A (vWA) domain) [General function prediction only].	8.64158e-53
NZ_CP016629.1\|WP_003728096.1\|505106_505862_-\|DUF4866-domain-containing-protein	gnl\|CDD\|379782	pfam16160, DUF4866, Domain of unknown function (DUF4866). This family consists of uncharacterized proteins around 250 residues in length and is mainly found in various human gut Firmicute species and abundant in human gut metagenomic datasets. The function of this family is unknown.	1.04021e-122
NZ_CP016629.1\|WP_003728093.1\|513403_514504_+\|DUF917-domain-containing-protein	gnl\|CDD\|226065	COG3535, COG3535, Uncharacterized conserved protein [Function unknown].	0
NZ_CP016629.1\|WP_003727314.1\|529744_530104_+\|DUF3139-domain-containing-protein	gnl\|CDD\|371476	pfam11337, DUF3139, Protein of unknown function (DUF3139). This family of proteins with unknown function appears to be restricted to Firmicutes.	1.02958e-22
NZ_CP016629.1\|WP_089179989.1\|518483_520490_+\|BspA-family-leucine-rich-repeat-surface-protein	gnl\|CDD\|308794	pfam03382, DUF285, Mycoplasma protein of unknown function, DUF285. This region appears distantly related to leucine rich repeats.	3.93028e-31
NZ_CP016629.1\|WP_003721242.1\|509287_510235_-\|MoxR-family-ATPase	gnl\|CDD\|223786	COG0714, COG0714, MoxR-like ATPases [General function prediction only].	7.47241e-102
NZ_CP016629.1\|WP_003728992.1\|512119_513403_+\|cytosine-permease	gnl\|CDD\|271377	cd11484, SLC-NCS1sbd_CobB-like, nucleobase-cation-symport-1 (NCS1) transporter CobB-like; solute-binding domain. This NCS1 subfamily includes Escherichia coli CodB (cytosine permease), and the Saccharomyces cerevisiae transporters: Fcy21p (Purine-cytosine permease), and vitamin B6 transporter Tpn1. NCS1s are essential components of salvage pathways for nucleobases and related metabolites; their known substrates include allantoin, uracil, thiamine, and nicotinamide riboside. NCS1s belong to a superfamily which also contains the solute carrier 5 family sodium/glucose transporters (SLC5s), and solute carrier 6 family neurotransmitter transporters (SLC6s).	1.15029e-95
NZ_CP016629.1\|WP_012681110.1\|528889_529717_+\|hypothetical-protein	gnl\|CDD\|368573	pfam05708, Peptidase_C92, Permuted papain-like amidase enzyme, YaeF/YiiX, C92 family. Amidase_YiiX is a family of permuted papain-like amidases. It has amidase specificity for the amide bond between a lipid and an amino acid (or peptide). From the structure, a tetramer, each monomer is made up of a layered alpha-beta fold with a central, 6-stranded, antiparallel beta-sheet that is protected by helices on either side. The catalytic Cys154 in UniProtKB:Q74NK7, Structure 3kw0, is located on the N-terminus of helix alphaF. The two additional helices located above Cys154 contribute to the formation of the active site, where the lysine ligand is bound.	0.00019582
NZ_CP016629.1\|WP_003727318.1\|523766_524072_+\|LPXTG-cell-wall-anchor-domain-containing-protein	gnl\|CDD\|273478	TIGR01167, Zinc_metalloprotease_ZmpB, LPXTG-motif cell wall anchor domain. This model describes the LPXTG motif-containing region found at the C-terminus of many surface proteins of Streptococcus and Streptomyces species. Cleavage between the Thr and Gly by sortase or a related enzyme leads to covalent anchoring at the new C-terminal Thr to the cell wall. Hits that do not lie at the C-terminus or are not found in Gram-positive bacteria are probably false-positive. A common feature of this proteins containing this domain appears to be a high proportion of charged and zwitterionic residues immediatedly upstream of the LPXTG motif. This model differs from other descriptions of the LPXTG region by including a portion of that upstream charged region. [Cell envelope, Other].	4.34504e-05

>NZ_CP016629.1|WP_089179989.1|518483_520490_+|BspA-family-leucine-rich-repeat-surface-protein
MKKFSMRVVLIISVLFIALGNANVSIAQERDTTNKLPEEELGSLDTSNLIAEEVAQEKPAEVEQLEEIPTTDELMQNPDVREQPVADSDDPDLTVVSSGDFWTLYYNSANDEYSLRMFGNVPSSKPSAWNSYLKRIKHIEIEEATLTGNFVYYFRGTVFPVLESVRIEQCNLSGVTSFARAFEGDSESESPLEKVIIRDNDYPETPSLTDISRMFVLCRKLSELDVSGLNTSSVTNMSTVFGNTNSLKELDISNFDTSSVTDMSYMFAACESLEELDLSTLDTSSVTNMYSMFSYCYNFEELDLSNFDTSSVTNMHLMFGACKSLEELDLSTFDTSSVANMRGMFGECNSLEKLDLSTFDTSSVTNMQIMFVECTSLEELDLSTFDTSSVTNMDRMFENSTALKSLYLDNFTDAASMTDMFKGTTSLTYLFVSHNLSTFNRLENTSWYDEKNWVQFSNLSQLQTYHRKQSEPTGYRKGAFLSLTMDAMGGEFEEMEEQKVQNKVSGEYWEEMLPVKEGHYFDGWYLDQNFTNKFDFSLPATVSATIYAKWVENYTVIIPASISLNEASELKVEGINRGGKTLSVGLNYGKTTISESNKLTLSNTADTTVQCLAPLSWDGSETNPKNAILTLAPGSEITEGDAVMAIEAPENIQAGKYTGNLVFSINYE
>NZ_CP016629.1|WP_061391596.1|516681_518220_+|helix-turn-helix-domain-containing-protein
MREYLDSKSQKKVALLEKIFYAENHTSTQEELLNDLNITYPTLISTIKTINFDIERFGYKAFSIVHSAPNLSYTLKISDNCSIQLIINAYIRESPKFQILETLLLSSFPNLQALAKKVHVSYSGIKKEIKELNEELSERNLSISTGNQVEITGDEFSLRIFYAFLFLVAYSGDRWPFSFVRYDEITDLLESCPKEIYRANSIDKAMMIHYYVAMHLLRDRMNCQIDTTRQFKVALYKACTEESKKSESAFIKKVAKQLPNRNYKEMTYTTQIILSTIVAFGSYSSIEKMPSFFYMDEQLEEMGFMKLVDFASERVNDNLSIPFSEKEMELLRYSFASINYRYFLLDNLINKFNNIVPGYTDLDRNIRKIHKVNHLEPLISQLVNLKEMDSLKPFEERLTSDYLIILDKRIDFSIHTLPIKVTILSTISNETAVFDFMRYFSSYYNLEIINQVDPVVDLYISDFSVSPEVLTSLRINQPIIYVNTRWLESDYVKINDNLAKIARKKFIANKKD
>NZ_CP016629.1|WP_003728092.1|514496_516047_+|hydantoinase/oxoprolinase-family-protein
MYKIGIDVGGTNTDAVILDENQQLIHSVKMPTSEDIETGITESLHRVLSETGIDRSKVTHAMLGTTQCTNAIVERKKLAKVGVLRLGYPATASVLPYTAWPEDMVGFLSGKYKLTGGGYEYDGQVLSEINEAEIRETLASWKGEVESVAVVGVFSSLKNDQELAVQKIIEEELGADIPVSISSSVGSVGLIERENATILNAALFKVIAATSDGFEKALEQEGIAHAEIYLCQNDGTLMSLNYARQFPILTIACGPTNSIRGASYLAGLKDAMVLDVGGTTSDIGVLTDGFPRESSLAVDVGGVRTNFRMPDIISIGLGGGSIVREKDGDITIGPDSVGYRISEEALVFGGKTMTTTDIAVRLGRAEIGNPELVAHIPTDFAEKAYKKIAEMTEDAIDKMKTSSGSVSLVLVGGGSVIIPDEISGVAQIFRDKNGPVANAIGASISQISGQYEQIYIYSQIEREEALQDAEQKAREQATLAGAVTDSIEVVEVEEIPLAYHPGNATRLRVKVVGNLV
>NZ_CP016629.1|WP_003728093.1|513403_514504_+|DUF917-domain-containing-protein
MRYLDEQAIENIAIGAAFLGTGGGGDPYIGKMMALTAIKKHGPIKLLSPEEIADDDYFIPAAMMGAPSVLIEKFPKGDEFVRVFEKLGRYVGKEVKGTFPMEAGGVNSMIPIVVAAQLGLPMVDCDGMGRAFPELQMVTFHLDGISATPMAITDEKGNIGIMETIDNKWTERLARVTTVEMGASSLVSLYPCNGAQIKQSSIKNIVTLSEEIGKVIRETSMDEAEKLQKLLDVTHGYHLFEGKITDVVRETRAGFNFGRVKIDGLNKDTGSEVIVHFQNENLVAEKNGEPIAITPDLICMVDLETLMPVTTEALKYGKRVRVMALPADDAWRTDKGIETVGPRYFGYDVDFEPLEELVKKEERRHV
>NZ_CP016629.1|WP_003728992.1|512119_513403_+|cytosine-permease
MTEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFMIYQGMQSSDLGIPAVSVASQVFGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGIYGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVLLFVCIYGLVVSLNDGGLAIVENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITVGAVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYHKVAVAVAGGLGTILAVIGILNHFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVSGVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGIILSLAVYLIGAKVLSGKTNNVENLEGK
>NZ_CP016629.1|WP_003740428.1|510390_511986_+|PucR-family-transcriptional-regulator
MSVKLADLMKLPSLKEAKVVSGKSGLSKLVSSISVLEYTEVALLEDDLFDNDEFYGSEIVVSAFVNIRDDVEAQCRTIERLHKVGEVALILYYVGIFVPKMDQKLIDFANELDFTIIVMPENEMSLRYSEVIYEVVEAIVKQEMTDTNFVTESLEQISSVRPPQRNIDTTLKILSDRTHTSLVLTDNSFQVINSITWPRTRHWDFERIIEASKQIGEHELVQLTLDERDFYTARKPIFQDGMQAMHLFMMKEKGVLTADVVMQITEVVQVFMNLWGRNYVEISTAELMKAILNDESVKMRRLGKILNVDVSSIKWMWLVKTEEIRDNEQVLAELKSFVASHFQVSLIDLFEDNIVVLLDNSVAQRDRTHTANEFAMMMKEHGIDLKITVCQGMEQTSDVQSAYSLVNEYKNAANAIYANKSIYSLQEIDFAAKCVEIVDRGELAIAEQMRPLKPLEDNDELIETLSVFLLEGESNYNQAAELLFLHKNTIKYRIQRINELLQYPATKIPESYNLYLAVAIRRILGGNNNNK
>NZ_CP016629.1|WP_003721242.1|509287_510235_-|MoxR-family-ATPase
MSIEPSIKIEEIINEVEKVIVGKRHVVKLSLTALLAGGHVLLEDVPGVGKTMMVRTLSKTIGVSFKRIQFTPDLLPADVTGVSIFNPETRDFEFRPGPVMGNIVLADEINRTAPRTQAALLEGMAENSVTVDGITRKLANPFFVMATQNPIEYEGTYALPEAQLDRFLLKINIGYPTVEEEMQLLTLKQYQDPLDQTQQVVTLSELLDLKNKAKQVFIDDVLKNYIIRLVDATRKHPSVALGISPRGSLAFMQAAQAFALIEGRDYVIPDDIQYLAPYIFTHRLILKSEAYYNEETAESIIASILKNTSVPVEKR
>NZ_CP016629.1|WP_031644083.1|508134_509283_-|DUF58-domain-containing-protein
MLKKRLLLAFRWIIMLIIYAGLWTYTLFQGDTASWFLTYFFSFILLLLFLSSIYPLKAWKVNRRFVKNTYHDGDLVDMQVSFTRKSRYPVGYLLFQQKIPASFGNVSARQQVVYPLFKKHFTVTLAGFVGVRGIHSFPPVDVETSDAFGLLERSRQLSSEKSLTIYPTYFPDVLEKISEASPENERNAAYWTLKKNSNLHSLREFSSGDRISLIDWKSSAKSDQLMTREFENSATSRLSVIFYGASHPNFELSLRAAYSFIRKISEDNGEIRVTLLGDQTSKFAPAKGTSKLQDISTAFAEIAPVDNLTLQESLDSNLHSGEKILLFTPCIDTMLMQKVTRLTDNKQLQIITFQSEHSKAMDAPAIFLEPASLLSRWEREAK
>NZ_CP016629.1|WP_003725186.1|505978_508138_-|transglutaminase
MKRYLTLVMLFILSVLLISEWIYPFAELTELSTANWIILFITLSLGSFFLRLKYYWTIPLHIVMIIFVSGIYYAKGGILAGNWFVSFFQITFNGFRDMVQFRSSDISMDFILIVFLIALWLLSYITTYSILRKQRVMSVLVLTVAYLAVINTFTDYNSNWAIVRTVLEGFLLLHLALASRIAAPNRISADSLKRNGLFYHIFVILLLAVFVFSSLMLPKKAPIFPDPVPTIKNALGINTTRTVGYSEDDTTLGGALKKDNATVFTARAEKGHYWRVESKTVYTGTGWANANSADVISFSSGDNFPIQLTEQTTGATNTAEISFASSNDYMPYPYGTQTVNGAVDSFSANLTIERVGPVDTIKNYTVQLKTPVYNIEKMQNADFSTLSNAFVSQYTQTPKDLPDRVTKLANKVTKDADSIYDKTKAIERYLSASGTFTYSTEDAKETPRGADYVDQFLFETQIGYCDNFSTSMVVMLRSLGIPARWAKGYTPGEGEKKENDAKTTYTITNNNAHSWPEVFFPGTGWVPFEPTATFSNPENFQEPTTETANKPETPNDSTSTPETPNTADKTPEQDNGSSSTGETPKKEEPKTETAASNFEIPSWVWWIPAGLVVTLLALAIIFRRRIRRYLLLRSLRNSPEFGKTYHRLMKLLASYGYVRESSETLRHFSTRVDAELTTTEFSRLTKMYEAQIYSNNLTEKVITAEETQLVQQVIARLTK
>NZ_CP016629.1|WP_003728096.1|505106_505862_-|DUF4866-domain-containing-protein
MTTIFPKEQNVAPLFEQILANPTACRRFKDVFLDNLESYQETRGFEKDDTLFAKIILSAYRQSDVSALLLGVCGRTLFELLRQAFLIPKKLTVDNPFFLTDKEGNFIAKKDDVSNREQEKFQEIYQSDLHHSETTIFLVDDDDIVHSYEPDFSISTKRINKKRGILVLYSLPDTLKLGMTESEVYAFIWKTFLHIQEIIPSSRIFYGQETSENADELGVFLPIHHFEKKMLQNIEQVNELVDALREQMVNK
>NZ_CP016629.1|WP_003728455.1|522760_523234_+|hypothetical-protein
MKKKRVVIISLLLLLVSVIGISSYFLFKDKINLLDVDHSAVEWNGKKQKDTSGEENTIAIPGFEKVTLYANETTQAVNFHNPEINDCYFKISLIHPDGSVLWISDLIEPGKGMYSIELEKTLAVGEYENAVLKYECFSLNDQSPLNGSEINLKLVVV
>NZ_CP016629.1|WP_003721253.1|523253_523739_+|hypothetical-protein
MNKLVLSTLSVAAMGMVFFSGGTAYAADKEGNTVVEYSVEGDYTLVVPEKVNLSNDNATEMSVKTINRNLEPGKEVEVTLSSGLSADGEIELQRVGAISDVITSSFKSNNSVVPMANPVIGSFSGYAMEETEVSKIQIGNPQGDKKAGAYQTTLTFTAAFK
>NZ_CP016629.1|WP_003727318.1|523766_524072_+|LPXTG-cell-wall-anchor-domain-containing-protein
MKRKLVLAMVLISFCGMFFLSPDRVQASQTVVEVLISEDDLDVSEENIQEIPKKELAASSDKDNKQPILPKAGDTVTNYTLLGLVLVLIWLIMQRKRKKKE
>NZ_CP016629.1|WP_014929391.1|524147_524414_+|transposase
MARIEYGEEIQKSLLVLYSRGSTIDSICREYGIPRYEFHKWMKLHDSDKLETKEVETFLQVRELKQQKNKLEEEILFLNEAIKLLESP
>NZ_CP016629.1|WP_003734772.1|525147_525765_+|hypothetical-protein
MLMSLSVLPLATYASETENTPTESYDGENFIATQTGNTLVIEDKKTGETVKIEMNDEENGVITSDDGTIENVHRDEEGNVYVDNELELEAPPLDIEDGINIATQPRLLKASKWIYVQTTKYNTTTQGNMRSLALGILSFMPITGPIFGIVAIIDAARSMGAKTLYVRVKQYRTSGYQFYKYDSYYYANASLTKLVKKTSQTKRMW
>NZ_CP016629.1|WP_003734773.1|526074_526422_+|hypothetical-protein
MQKDTYSGIRLSVIQLIDSKKENLKENNIKLTIIKDEKDGYVVELDNDKCMAEIVVEEPTYAPYRYISFEVVSLMDGKVKIIYSWYDDETSQWSDIEKELNKGIQFLNNFKTMEQ
>NZ_CP016629.1|WP_003734774.1|526825_527068_+|hypothetical-protein
MKRIILTSTLIVWTIVCIYMSISMVSNNTGIAFPIWLHIILLICFLATGIVNVKKKEYLWSTMLFEGVLVVLLSLIIVLV
>NZ_CP016629.1|WP_003734775.1|527150_528128_-|HD-domain-containing-protein
MKITDPLYGTFEIEPVLAELIQSPLVSRLAYVHQGGSSYLVNPLWDLSRLDHSIGVMLFIRKFGGSLEEQIAGLLHDVSHTAFSHVVDYALDFEEEDYHEQIFEDFVKTSTIPAILEKYGYSFEGIFDDISKWKILEQEAPELCADRIDYTLQDLYRHRKISLAEVEEFLQALVMVDGRLYLANIALAEWFVAQYYSEVIDYFYDPLNVYSYEILAEAMRIAFQQETITTADLRLTDTELLAKLNANSDAREKIQLLASAHLEENEADFTYHHKKKMRLINPSILVDGRLIPADELSEKVRKMNDWASERSKHGIYIKATKKSKQ
>NZ_CP016629.1|WP_012681110.1|528889_529717_+|hypothetical-protein
MKKRLIGIITALLLVSGILLSPQMAQAETKVDYDALYQQGVSEGIINKADVSLETWIKENESEYNQVYQDGLKDGVYDASLSYEEWIKLNNYGQPPVVDENWEEVPQKPMLKGVYKGYNIKKGDILITNGTSSSGLLGHAAIANGNEYILDIPGAGKTTRQVPTSKWMSDYDPEGWVKVYRLKDSSVANAAANWADKNYYSTKGTSKQNIFPKYGMTGSRYSKNPTYCSKIVLQAYYFGTGNKPVVQVFPSLVTVYDLPNYFSKAYKPQQVKYFK
>NZ_CP016629.1|WP_003727314.1|529744_530104_+|DUF3139-domain-containing-protein
MKKYKKWWITIGLVLIICIIGYIYWFAIPKHTANEAVDDYLAAQKINSTQVKTRDIQKDWTRGGYYVTIVFKDDPNLVYEYNYNSKRNTPYHINLLVFKDGSSQEDDQVKHPPLQEQKD

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP016629_1	1.2\|521400\|39\|NZ_CP016629\|CRT	521400-521438	39	NZ_CP016629.1	2130599-2130637	0	1.0

1. spacer 1.2|521400|39|NZ_CP016629|CRT matches to position: 2130599-2130637, mismatch: 0, identity: 1.0

tcttacatgttttatgataatagaaaccttgaggtgctg	CRISPR spacer
tcttacatgttttatgataatagaaaccttgaggtgctg	Protospacer
***************************************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

82714 : 90559

Listeria_phage(87.5%)

holin

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_031643969.1\|82714_83671_+	NAD(P)-binding domain-containing protein	M1H502	Paramecium_bursaria_Chlorella_virus	4.6e-29	29.6
WP_061112480.1\|85204_85993_-	hypothetical protein	A0A1S7FYW2	Listeria_phage	6.1e-35	67.0
WP_102135858.1\|86047_86215_-	hypothetical protein	NA	NA	NA	NA
WP_061112479.1\|86341_86818_-	helix-turn-helix transcriptional regulator	A8ASM2	Listeria_phage	6.2e-59	83.8
WP_060579670.1\|86952_87174_+	helix-turn-helix transcriptional regulator	A8ASM3	Listeria_phage	2.5e-31	95.9
WP_015987418.1\|87208_87445_+	hypothetical protein	A8ATX7	Listeria_phage	1.1e-37	100.0
WP_003733957.1\|89127_89433_+	hypothetical protein	A0A059T5E6	Listeria_phage	1.8e-43	100.0
WP_061112478.1\|89432_89714_+\|holin	phage holin	A8ATW3	Listeria_phage	5.7e-44	97.8
WP_061112477.1\|89713_90559_+	M15 family metallopeptidase	A0A059T7Y8	Listeria_phage	1.7e-136	94.0

DBSCAN-SWA_2

132170 : 138697

Listeria_phage(33.33%)

tail

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003728215.1\|132170_132623_-	ImmA/IrrE family metallo-endopeptidase	A0A059T7S0	Listeria_phage	4.0e-31	47.9
WP_003721740.1\|132628_132964_-	helix-turn-helix transcriptional regulator	A0A2H4JAR9	uncultured_Caudovirales_phage	4.0e-20	52.5
WP_003724946.1\|133180_133609_+	hypothetical protein	NA	NA	NA	NA
WP_003728213.1\|133620_134037_+	hypothetical protein	A8ASQ1	Listeria_phage	3.2e-19	37.9
WP_003728212.1\|134316_134706_+	DUF5072 family protein	NA	NA	NA	NA
WP_003721744.1\|134718_135231_+\|tail	phage major tail protein, TP901-1 family	A0A097PBF4	Streptococcus_pyogenes_phage	1.2e-47	62.7
WP_003721745.1\|135278_135581_+	hypothetical protein	NA	NA	NA	NA
WP_003740367.1\|135622_136027_+	hypothetical protein	NA	NA	NA	NA
WP_003731178.1\|136013_137882_+	membrane protein	A0A097PAU2	Streptococcus_pyogenes_phage	3.5e-20	45.8
WP_003731179.1\|137878_138697_+\|tail	phage tail family protein	A0A060AFE1	Staphylococcus_phage	5.5e-39	35.0

DBSCAN-SWA_3

1132225 : 1161286

Streptococcus_phage(66.67%)

transposase,integrase

attL 1118080:1118094|attR 1143325:1143339

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1118080:1118094	attL	NA	NA	NA	NA
WP_003727003.1\|1132225_1132609_+	glycerol-3-phosphate cytidylyltransferase	A0A1V0SGE7	Hokovirus	8.1e-17	40.7
WP_003727002.1\|1132630_1133614_+	glycosyltransferase family 2 protein	A0A1V0SAH6	Catovirus	2.0e-11	33.8
WP_003727001.1\|1133628_1134642_+	glycosyltransferase	A0A1V0SAH6	Catovirus	1.8e-10	31.2
WP_003721509.1\|1134850_1136341_+	nicotinate phosphoribosyltransferase	G3MA18	Bacillus_virus	9.5e-114	49.7
WP_003727000.1\|1136352_1137177_+	ammonia-dependent NAD(+) synthetase	G3MA24	Bacillus_virus	9.7e-68	52.6
WP_003724634.1\|1137189_1137498_+	antibiotic biosynthesis monooxygenase	NA	NA	NA	NA
WP_009917597.1\|1137557_1137962_+	PTS sugar transporter subunit IIB	NA	NA	NA	NA
WP_012681222.1\|1138090_1139647_+	glutamine-hydrolyzing GMP synthase	A0A1V0SH76	Hokovirus	2.3e-17	28.1
WP_000237798.1\|1139714_1140908_-\|integrase	site-specific integrase	A0A0S2MV79	Bacillus_phage	2.4e-43	34.0
WP_000633909.1\|1140934_1141135_-	DUF3173 domain-containing protein	NA	NA	NA	NA
WP_000845144.1\|1141630_1141861_-	helix-turn-helix domain-containing protein	A0A1S5SEX1	Streptococcus_phage	7.9e-28	78.9
WP_000804878.1\|1141857_1142280_-	sigma-70 family RNA polymerase sigma factor	A0A1S5SEW0	Streptococcus_phage	1.0e-49	70.0
WP_089180001.1\|1142811_1143165_+	helix-turn-helix domain-containing protein	A0A1S5SFA6	Streptococcus_phage	1.4e-52	89.7
WP_032506803.1\|1143222_1143411_-	cysteine-rich KTR domain-containing protein	D0R0F6	Streptococcus_phage	7.4e-16	61.4
1143325:1143339	attR	NA	NA	NA	NA
WP_000691737.1\|1143508_1145428_-	tetracycline resistance ribosomal protection protein Tet(M)	A0A1S5SF82	Streptococcus_phage	0.0e+00	97.2
WP_077941411.1\|1145443_1145560_-	tetracycline resistance determinant leader peptide	NA	NA	NA	NA
WP_000584387.1\|1145804_1146734_-	conjugal transfer protein	A0A1S5SF22	Streptococcus_phage	1.1e-83	56.6
WP_000768374.1\|1146750_1147773_-	lysozyme family protein	A0A1S5SEZ8	Streptococcus_phage	1.4e-132	75.5
WP_000192390.1\|1147769_1149797_-	hypothetical protein	A0A1S5SF30	Streptococcus_phage	1.8e-195	65.7
WP_000941001.1\|1149793_1152247_-	ATP-binding protein	A0A1S5SF64	Streptococcus_phage	0.0e+00	79.1
WP_000723886.1\|1152230_1152626_-	conjugal transfer protein	A0A1S5SEX7	Streptococcus_phage	1.7e-49	75.0
WP_000234705.1\|1152696_1153722_-	hypothetical protein	NA	NA	NA	NA
WP_000195404.1\|1153800_1154967_-\|transposase	IS256 family transposase	A0A0N9STL0	Staphylococcus_phage	1.2e-106	76.0
WP_000421245.1\|1155129_1155630_-	antirestriction protein ArdA	A0A1S5SF25	Streptococcus_phage	5.4e-53	61.1
WP_000678782.1\|1155691_1156471_-	abortive infection family protein	NA	NA	NA	NA
WP_001009054.1\|1156512_1156734_-	hypothetical protein	A0A1S5SEY0	Streptococcus_phage	3.1e-29	93.2
WP_000055367.1\|1156730_1157003_-	hypothetical protein	NA	NA	NA	NA
WP_000426691.1\|1156999_1158184_-	replication initiation factor domain-containing protein	A0A1S5SEX3	Streptococcus_phage	1.0e-158	67.6
WP_001130247.1\|1158365_1159769_-	DNA translocase FtsK	A0A1S5SFB5	Streptococcus_phage	2.3e-178	67.2
WP_000185760.1\|1159790_1160564_-	hypothetical protein	NA	NA	NA	NA
WP_001234189.1\|1160573_1160951_-	YdcP family protein	A0A1S5SF96	Streptococcus_phage	5.6e-47	77.2
WP_000421280.1\|1160971_1161286_-	YdcP family protein	A0A1S5SF38	Streptococcus_phage	7.0e-43	76.7

DBSCAN-SWA_4

1306504 : 1363354

Bacillus_virus(16.67%)

protease,tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_031643879.1\|1306504_1307644_-\|protease	PrsW family intramembrane metalloprotease	NA	NA	NA	NA
WP_003723563.1\|1307723_1308119_-	helix-turn-helix transcriptional regulator	A9D9J6	Lactobacillus_prophage	1.3e-14	57.4
WP_003727524.1\|1308269_1308485_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_003727523.1\|1308608_1309142_+\|protease	matrixin family metalloprotease	NA	NA	NA	NA
WP_003731300.1\|1309157_1309823_+	hypothetical protein	NA	NA	NA	NA
WP_003727521.1\|1310084_1311023_+	hypothetical protein	NA	NA	NA	NA
WP_003723884.1\|1311137_1312421_+	trigger factor	NA	NA	NA	NA
WP_003723886.1\|1312605_1313865_+\|protease	ATP-dependent protease ATP-binding subunit ClpX	G3M9Z9	Bacillus_virus	1.4e-145	63.2
WP_003726393.1\|1313983_1314550_+	type I signal peptidase SipX	NA	NA	NA	NA
WP_003726394.1\|1314584_1315154_+	type I signal peptidase SipY	NA	NA	NA	NA
WP_003727520.1\|1315255_1315798_+	type I signal peptidase SipZ	NA	NA	NA	NA
WP_003726396.1\|1315807_1316671_+	ribosome biogenesis GTPase YlqF	NA	NA	NA	NA
WP_003727519.1\|1316667_1317453_+	ribonuclease HII	G4YAY0	Emiliania_huxleyi_virus	6.7e-26	37.6
WP_003727518.1\|1317586_1318447_+	DNA-protecting protein DprA	NA	NA	NA	NA
WP_003727517.1\|1318719_1320798_+	type I DNA topoisomerase	A0A1V0SB35	Catovirus	5.4e-107	37.5
WP_003726401.1\|1320860_1322165_+\|tRNA	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	NA	NA	NA	NA
WP_023550485.1\|1322447_1323350_+	tyrosine recombinase XerC	A0A142K7N4	Mycobacterium_phage	1.8e-14	29.5
WP_003724001.1\|1323370_1323910_+\|protease	ATP-dependent protease subunit HslV	NA	NA	NA	NA
WP_003727514.1\|1323923_1325333_+\|protease	ATP-dependent protease ATPase subunit HslU	W6AS21	Erwinia_phage	7.5e-44	27.8
WP_003726695.1\|1325353_1326133_+	GTP-sensing pleiotropic transcriptional regulator CodY	NA	NA	NA	NA
WP_003727513.1\|1326235_1326655_+	acyl-CoA thioesterase	NA	NA	NA	NA
WP_003724130.1\|1326677_1326989_+	hypothetical protein	NA	NA	NA	NA
WP_003726697.1\|1326991_1327864_+	aldose 1-epimerase family protein	NA	NA	NA	NA
WP_003726698.1\|1327905_1328502_-	glycerol-3-phosphate 1-O-acyltransferase PlsY	NA	NA	NA	NA
WP_003731304.1\|1328659_1329067_+	CoA-binding protein	NA	NA	NA	NA
WP_003727510.1\|1329247_1331215_+	DNA topoisomerase IV subunit B	G3M9Z3	Bacillus_virus	5.1e-123	42.5
WP_010958880.1\|1331211_1333671_+	DNA topoisomerase IV subunit A	G3M9Z5	Bacillus_virus	1.1e-101	31.8
WP_003726814.1\|1333753_1334221_+	S-ribosylhomocysteine lyase	NA	NA	NA	NA
WP_061112335.1\|1334847_1336668_+	class 1 internalin InlK	NA	NA	NA	NA
WP_003727507.1\|1336700_1338587_-	peptidoglycan O-acetyltransferase OatA	B5WZU0	Pseudomonas_phage	3.8e-43	32.2
WP_003726657.1\|1338799_1339498_+	glycerophosphodiester phosphodiesterase	A0A0S2MYI4	Enterococcus_phage	6.4e-12	31.9
WP_003723738.1\|1339730_1341407_+	glycerol-3-phosphate dehydrogenase/oxidase	NA	NA	NA	NA
WP_003726658.1\|1341533_1342451_+\|tRNA	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	NA	NA	NA	NA
WP_003719566.1\|1342572_1342806_+	RNA chaperone Hfq	NA	NA	NA	NA
WP_012681271.1\|1342916_1344140_+	GTPase HflX	NA	NA	NA	NA
WP_003727505.1\|1344132_1345359_+	methionine gamma-lyase family protein	NA	NA	NA	NA
WP_003719570.1\|1345560_1345929_+	MerR family transcriptional regulator	NA	NA	NA	NA
WP_003723435.1\|1345999_1347334_+	type I glutamate--ammonia ligase	A0A1V0SJ53	Klosneuvirus	2.0e-06	26.4
WP_023550496.1\|1347477_1348773_+	arsenic transporter	A0A2H4PQU3	Staphylococcus_phage	2.2e-146	62.1
WP_003734519.1\|1348806_1349331_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_009928625.1\|1349361_1349976_-	transcriptional repressor LexA	A0A1B2APZ1	Phage_Wrath	9.3e-15	62.1
WP_003734518.1\|1350131_1350461_+	cell division suppressor protein YneA	NA	NA	NA	NA
WP_003727501.1\|1350554_1350782_+	DUF896 domain-containing protein	NA	NA	NA	NA
WP_009928628.1\|1350928_1352923_+	transketolase	NA	NA	NA	NA
WP_003726667.1\|1353143_1353383_+	YneF family protein	NA	NA	NA	NA
WP_003726668.1\|1353431_1354163_-	class I SAM-dependent methyltransferase	A0A1X9I6N4	Streptococcus_phage	2.3e-81	58.0
WP_003726669.1\|1354184_1354691_-	ParB-like nuclease domain-containing protein	A0A220GKT8	Streptococcus_phage	1.4e-56	66.7
WP_021496023.1\|1354700_1356005_-	DUF3440 domain-containing protein	A0A220GKF8	Streptococcus_phage	5.6e-134	52.3
WP_012681273.1\|1355994_1357200_-	helicase SNF2	L0P6E9	Lactobacillus_phage	7.0e-91	46.6
WP_003727498.1\|1357177_1357552_-	hypothetical protein	NA	NA	NA	NA
WP_003723449.1\|1357844_1358573_+	UMP kinase	NA	NA	NA	NA
WP_003723450.1\|1358572_1359130_+	ribosome recycling factor	NA	NA	NA	NA
WP_089180006.1\|1359360_1360119_+	isoprenyl transferase	R9W0U9	Flavobacterium_phage	5.7e-22	42.1
WP_003727496.1\|1360132_1360921_+	phosphatidate cytidylyltransferase	NA	NA	NA	NA
WP_003726414.1\|1360935_1362078_+	1-deoxy-D-xylulose-5-phosphate reductoisomerase	NA	NA	NA	NA
WP_061112334.1\|1362091_1363354_+\|protease	RIP metalloprotease RseP	NA	NA	NA	NA

DBSCAN-SWA_5

1854443 : 1862729

Synechococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003726209.1\|1854443_1855010_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	8.3e-26	36.4
WP_003726210.1\|1855006_1856056_-	phosphoribosylformylglycinamidine cyclo-ligase	Q58MH8	Prochlorococcus_phage	3.2e-63	44.4
WP_003722245.1\|1856074_1857502_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.1e-53	34.6
WP_003731570.1\|1857486_1859706_-	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	6.5e-159	40.8
WP_003726212.1\|1859698_1860382_-	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_003722248.1\|1860385_1860631_-	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
WP_031644139.1\|1860642_1861356_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3F9V5	Synechococcus_phage	3.9e-41	38.3
WP_089180017.1\|1861436_1862729_-	adenylosuccinate lyase	A0A1B3B081	Gordonia_phage	2.5e-17	33.0

DBSCAN-SWA_6

2539456 : 2547298

Streptococcus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003725407.1\|2539456_2540428_-	DNA-binding protein WhiA	Q7AWZ3	Streptococcus_phage	5.2e-52	39.4
WP_003731212.1\|2540435_2541404_-	YvcK family protein	A1IMD5	Streptococcus_phage	1.8e-68	43.6
WP_003722606.1\|2541405_2542281_-	RNase adapter RapZ	A0A0R8VB27	Thermobifida_phage	1.9e-08	29.7
WP_003725409.1\|2542388_2544119_-	phospho-sugar mutase	A0A1X9I671	Streptococcus_phage	9.5e-174	53.1
WP_003741152.1\|2544160_2545222_-	galactose mutarotase	NA	NA	NA	NA
WP_014929803.1\|2545238_2546222_-	UDP-glucose 4-epimerase GalE	A0A2K9L5H6	Tupanvirus	4.0e-52	38.4
WP_003722610.1\|2546338_2547298_-	thioredoxin-disulfide reductase	G3MA85	Bacillus_virus	2.0e-88	53.7

DBSCAN-SWA_7

2828875 : 2838899

Tupanvirus(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_009917426.1\|2828875_2831029_-	DNA topoisomerase III	A0A1X9I6W8	Streptococcus_phage	3.3e-43	33.8
WP_031644014.1\|2831052_2832825_-	DNA helicase RecQ	A0A2K9L3P7	Tupanvirus	5.7e-81	37.5
WP_003722118.1\|2832985_2834452_-	IMP dehydrogenase	A0A1V0SHK8	Klosneuvirus	8.0e-97	40.0
WP_003725312.1\|2834739_2835270_-	ADP-ribose-binding protein	A0A0K1L687	Scale_drop_disease_virus	2.8e-28	49.3
WP_003725313.1\|2835327_2836938_-	ABC-F family ATP-binding cassette domain-containing protein	A0A2K9L3Z8	Tupanvirus	6.4e-47	29.9
WP_003725314.1\|2837444_2838899_+	glycoside hydrolase family 1 protein	A0A0B5JD41	Pandoravirus	1.9e-50	30.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage