CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_LN999830	Bacillus velezensis strain B25 plasmid pBAMMD1, complete sequence	0 crisprs	NA	0	0	0	0
NZ_LN999829	Bacillus velezensis strain B25 chromosome BAMMD1	0 crisprs	csa3,DinG,casR,cas3,DEDDh,WYL	0	0	6	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

630033 : 639924

Synechococcus_phage(50.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_007408896.1\|630033_631326_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	4.5e-19	27.3
WP_003155762.1\|631401_632121_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3G7P8	Synechococcus_phage	5.2e-49	45.2
WP_014417102.1\|632120_632375_+	phosphoribosylformylglycinamidine synthase subunit PurS	M4QPE7	Synechococcus_phage	4.7e-05	33.3
WP_015388591.1\|632371_633055_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_003155755.1\|633038_635267_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	3.2e-158	40.4
WP_003155754.1\|635242_636673_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.6e-54	32.9
WP_015388588.1\|636764_637805_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	1.4e-63	41.1
WP_003155752.1\|637801_638389_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.0e-26	39.3
WP_003155751.1\|638385_639924_+	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	8.7e-78	50.8

DBSCAN-SWA_2

1087451 : 1121062

Planktothrix_phage(16.67%)

tRNA,coat,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_060386866.1\|1087451_1088444_-\|tRNA	tryptophan--tRNA ligase	NA	NA	NA	NA
WP_012117282.1\|1089187_1090822_+	peptide ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_003155043.1\|1090928_1091864_+	ABC transporter permease	NA	NA	NA	NA
WP_003155041.1\|1091867_1092785_+	oligopeptide ABC transporter permease OppC	NA	NA	NA	NA
WP_003155039.1\|1092797_1093874_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	1.7e-16	29.9
WP_003155037.1\|1093866_1094784_+	oligopeptide ABC transporter ATP-binding protein OppF	M1HP82	Acanthocystis_turfacea_Chlorella_virus	1.5e-05	24.6
WP_003155036.1\|1094890_1096078_+	putative glycoside hydrolase	NA	NA	NA	NA
WP_003155035.1\|1096195_1096774_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_003155034.1\|1096952_1097348_+	transcriptional regulator Spx	NA	NA	NA	NA
WP_003155033.1\|1097405_1098062_-	TerC family protein	A0A0S4KZH7	Pseudomonas_phage	3.8e-30	40.4
WP_003155032.1\|1098337_1098994_+	adaptor protein MecA	NA	NA	NA	NA
WP_060386867.1\|1099144_1100305_+	competence protein CoiA	NA	NA	NA	NA
WP_003155028.1\|1100532_1102362_+	oligoendopeptidase F	NA	NA	NA	NA
WP_003155026.1\|1102399_1102567_-	hypothetical protein	NA	NA	NA	NA
WP_024085126.1\|1102852_1103755_-\|protease	protease adaptor protein SpxH	NA	NA	NA	NA
WP_003155023.1\|1103751_1104150_-	thiol management oxidoreductase	NA	NA	NA	NA
WP_003155022.1\|1104378_1105065_-	lytic transglycosylase domain-containing protein	A0A1P8CWQ1	Bacillus_phage	7.9e-39	71.2
WP_015388447.1\|1105069_1105645_-	CYTH domain-containing protein	NA	NA	NA	NA
WP_015388446.1\|1105769_1106135_+	hypothetical protein	NA	NA	NA	NA
WP_003155019.1\|1106162_1106798_+	GTP diphosphokinase	NA	NA	NA	NA
WP_003155018.1\|1106819_1107620_+	NAD kinase	NA	NA	NA	NA
WP_003155017.1\|1107634_1108528_+	RluA family pseudouridine synthase	A0A2H4UV25	Bodo_saltans_virus	1.0e-06	29.3
WP_057080088.1\|1108561_1109311_-	bis(5'-nucleosyl)-tetraphosphatase PrpE	A0A1V0SJW2	Klosneuvirus	1.2e-11	26.7
WP_003155014.1\|1109538_1111383_+	monovalent cation:proton antiporter family protein	NA	NA	NA	NA
WP_060387293.1\|1111632_1112340_+	thiaminase II	NA	NA	NA	NA
WP_043866990.1\|1112317_1112935_+	thiazole tautomerase TenI	NA	NA	NA	NA
WP_060386868.1\|1112918_1114028_+	glycine oxidase ThiO	NA	NA	NA	NA
WP_003155008.1\|1114024_1114228_+	sulfur carrier protein ThiS	NA	NA	NA	NA
WP_088005363.1\|1114224_1114995_+	thiazole synthase	NA	NA	NA	NA
WP_060386869.1\|1114991_1116002_+	thiazole biosynthesis adenylyltransferase ThiF	NA	NA	NA	NA
WP_014304798.1\|1116024_1116837_+	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	NA	NA	NA	NA
WP_003155001.1\|1116967_1117744_+	enoyl-ACP reductase FabI	NA	NA	NA	NA
WP_170936232.1\|1117835_1118450_+\|coat	spore coat protein	NA	NA	NA	NA
WP_003154997.1\|1118508_1118952_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003154995.1\|1119097_1119580_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003154994.1\|1119730_1120231_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003154993.1\|1120323_1120638_-	hypothetical protein	NA	NA	NA	NA
WP_003154992.1\|1120675_1121062_-\|coat	spore coat protein	NA	NA	NA	NA

DBSCAN-SWA_3

1191062 : 1223202

Bacillus_phage(33.33%)

portal,capsid,terminase,holin,plate,tail

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_087920760.1\|1191062_1192199_+	S9 family peptidase	A0A1P8CWN8	Bacillus_phage	1.3e-94	47.9
WP_003154881.1\|1192188_1192323_+	PhrA family phosphatase inhibitor	NA	NA	NA	NA
WP_060386892.1\|1192465_1193419_+	N-acetylmuramoyl-L-alanine amidase	A0A218KC88	Bacillus_phage	1.6e-61	68.7
WP_003154878.1\|1193456_1193834_-	PH domain-containing protein	A5GYQ0	Lactococcus_phage	1.1e-15	40.6
WP_014304840.1\|1193943_1194549_+	poly-gamma-glutamate hydrolase family protein	A0A0Y0AJU6	Bacillus_phage	1.3e-40	46.8
WP_162885922.1\|1194538_1194688_+	hypothetical protein	NA	NA	NA	NA
WP_003154873.1\|1194703_1195294_-	ImmA/IrrE family metallo-endopeptidase	A0A2H4JA43	uncultured_Caudovirales_phage	3.0e-39	51.7
WP_003154871.1\|1195442_1195781_-	helix-turn-helix transcriptional regulator	A0A2H4J6F9	uncultured_Caudovirales_phage	8.4e-18	45.8
WP_003154869.1\|1195972_1196152_+	hypothetical protein	NA	NA	NA	NA
WP_048367154.1\|1196141_1196969_+\|portal	phage portal protein	S6BFM4	Thermus_phage	1.7e-19	49.0
WP_043867013.1\|1196868_1197669_+	ATP-binding protein	A6XMI1	Bacillus_virus	1.2e-59	45.5
WP_003154863.1\|1197668_1197836_+	hypothetical protein	NA	NA	NA	NA
WP_003154861.1\|1197933_1198275_+	hypothetical protein	NA	NA	NA	NA
WP_043867014.1\|1198264_1198468_+	hypothetical protein	A0A2H4J4M6	uncultured_Caudovirales_phage	7.0e-12	47.0
WP_012117362.1\|1198580_1199093_+	sigma-70 family RNA polymerase sigma factor	A0A0K2CNQ1	Brevibacillus_phage	2.9e-22	44.6
WP_003154855.1\|1199205_1200003_+\|terminase	terminase small subunit	A0A0S2MVB6	Bacillus_phage	9.4e-60	50.6
WP_060386893.1\|1199999_1201298_+\|terminase	PBSX family phage terminase large subunit	M4ZRM5	Bacillus_phage	2.5e-150	59.4
WP_099762611.1\|1201346_1202726_+\|portal	phage portal protein	A0A1B1P7C8	Bacillus_phage	5.1e-138	55.4
WP_015417286.1\|1202757_1203603_+	hypothetical protein	A0A1B1P7E4	Bacillus_phage	4.3e-55	57.6
WP_003154848.1\|1203629_1204565_+\|capsid	phage major capsid protein	A0A1B1P7E3	Bacillus_phage	2.5e-104	62.8
WP_015388357.1\|1204581_1204965_+	DUF3199 family protein	NA	NA	NA	NA
WP_003154844.1\|1204961_1205318_+	DUF3599 family protein	NA	NA	NA	NA
WP_014304847.1\|1205314_1205818_+	HK97 gp10 family phage protein	NA	NA	NA	NA
WP_014304848.1\|1205814_1206261_+	hypothetical protein	NA	NA	NA	NA
WP_003154839.1\|1206257_1206467_+	hypothetical protein	NA	NA	NA	NA
WP_044053125.1\|1206466_1207864_+\|tail	phage tail sheath subtilisin-like domain-containing protein	A0A0A7RTT5	Clostridium_phage	9.0e-82	40.4
WP_003154837.1\|1207865_1208309_+\|tail	phage tail tube protein	A0A0K2CNG3	Brevibacillus_phage	8.4e-26	45.2
WP_003154836.1\|1208385_1208832_+\|portal	phage portal protein	A0A0A7RTY8	Clostridium_phage	1.0e-10	35.8
WP_015239684.1\|1208873_1209026_+	hypothetical protein	NA	NA	NA	NA
WP_060386894.1\|1209013_1214380_+	transglycosylase SLT domain-containing protein	A0A1L2JY60	Aeribacillus_phage	2.1e-41	46.6
WP_007610816.1\|1214372_1215032_+	LysM peptidoglycan-binding domain-containing protein	G3MBQ1	Bacillus_virus	1.8e-08	45.3
WP_060386895.1\|1215045_1216023_+	hypothetical protein	A0A1L6BY20	Clostridium_phage	8.3e-34	29.8
WP_007610818.1\|1216022_1216289_+	DUF2577 family protein	S6C459	Thermus_phage	1.2e-06	38.6
WP_003154825.1\|1216392_1216818_+	DUF2634 domain-containing protein	A0A2H4J6K5	uncultured_Caudovirales_phage	1.5e-11	35.9
WP_003154824.1\|1216810_1217857_+\|plate	baseplate J/gp47 family protein	S6AVU3	Thermus_phage	7.7e-70	44.1
WP_003154823.1\|1217840_1218419_+	YmfQ family protein	A0A2H4J717	uncultured_Caudovirales_phage	2.4e-12	31.0
WP_014304854.1\|1218415_1218688_+	hypothetical protein	NA	NA	NA	NA
WP_024085192.1\|1218690_1220313_+	hypothetical protein	A0A1P8CWR7	Bacillus_phage	5.3e-41	39.3
WP_024085193.1\|1220325_1220697_+	hypothetical protein	NA	NA	NA	NA
WP_003154819.1\|1220702_1220900_+	XkdX family protein	A0A2H4JAA1	uncultured_Caudovirales_phage	4.3e-14	61.8
WP_024085194.1\|1220956_1221718_+	hypothetical protein	NA	NA	NA	NA
WP_032866112.1\|1221769_1222033_+	hemolysin XhlA family protein	A0A2H4JD40	uncultured_Caudovirales_phage	5.2e-23	63.1
WP_003154813.1\|1222046_1222310_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	2.3e-23	65.5
WP_024085195.1\|1222323_1223202_+	N-acetylmuramoyl-L-alanine amidase	Q9ZXD7	Bacillus_phage	4.8e-81	60.5

DBSCAN-SWA_4

1771416 : 1777630

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014305044.1\|1771416_1771809_+	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	G3MBF1	Bacillus_virus	6.5e-30	59.1
WP_003154060.1\|1771768_1773871_+	class 1b ribonucleoside-diphosphate reductase subunit alpha	U5PY35	Bacillus_phage	0.0e+00	86.0
WP_003154059.1\|1773888_1774878_+	class 1b ribonucleoside-diphosphate reductase subunit beta	F8WQ21	Bacillus_phage	8.7e-156	83.6
WP_060386952.1\|1774926_1775547_+	hypothetical protein	A0A127AYS1	Bacillus_phage	3.2e-47	48.4
WP_060386953.1\|1775596_1776355_-	N-acetylmuramoyl-L-alanine amidase	E5DV68	Deep-sea_thermophilic_phage	1.4e-52	50.0
WP_015417523.1\|1776661_1777630_+	stage V sporulation protein K	G3MAX6	Bacillus_virus	8.0e-53	40.9

DBSCAN-SWA_5

2195933 : 2202186

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003153378.1\|2195933_2196527_-	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	2.6e-14	36.6
WP_003153377.1\|2196516_2197272_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	6.5e-10	25.3
WP_003153376.1\|2197479_2197569_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_003153375.1\|2197656_2198178_+	DUF309 domain-containing protein	NA	NA	NA	NA
WP_003153373.1\|2198243_2198618_-	GNAT family N-acetyltransferase RibT	NA	NA	NA	NA
WP_003153372.1\|2198734_2199199_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.2e-43	59.3
WP_003153371.1\|2199231_2200428_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	2.1e-116	54.9
WP_003153370.1\|2200442_2201090_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	1.5e-39	44.3
WP_014305330.1\|2201070_2202186_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	1.5e-55	36.6

DBSCAN-SWA_6

2552598 : 2612927

uncultured_Mediterranean_phage(25.0%)