CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
NZ_CP012421	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence	1 crisprs	NA	4	0
NZ_CP012422	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC23, complete sequence	0 crisprs	DEDDh	0	0
NZ_CP012420	Rickettsia amblyommatis strain Ac37 chromosome, complete genome	0 crisprs	cas3,DEDDh	0	2

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP012421_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP012421_1

2587-2809

Orphan

Consensus_repeat	Method
ATAGCACTTGCGAAGATATTGCCGGATACTAAAATTACA	CRISPRCasFinder
ATAGCACTTGCGAAGATATTGCCGGATACTAAAATTACACATCTTAATCTTGAGGGTAAT	PILER-CR

2 spacers

The CRISPR arrays of NZ_CP012421_1

>merge|NZ_CP012421|1|2587-2809|CRISPRCasFinder,PILER-CR
GGACAACTAGCTGCTATATTGCCGGATACTAAAATTACACATCTTAATCTTGGATACAACTGTATAGACGCTGCAGGAATCATAGCACTTGCGAAGATATTGCCGGATACTAAAATTACACATCTTAGTCTTGAGTTTAATAATGTAGACAATATTGGTGCAATAGCACTTGCGAAGATATTGCCGGATACTAAGATTACATATCTTAATCTTGAGGGTAATG

>NZ_CP012421|1|1|2587-2787|CRISPRCasFinder
GGACAACTAGCTGCTATATTGCCGGATACTAAAATTACA	CATCTTAATCTTGGATACAACTGTATAGACGCTGCAGGAATC
ATAGCACTTGCGAAGATATTGCCGGATACTAAAATTACA	CATCTTAGTCTTGAGTTTAATAATGTAGACAATATTGGTGCA
ATAGCACTTGCGAAGATATTGCCGGATACTAAGATTACA

>NZ_CP012421|1|1|2602-2809|PILER-CR
ATATTGCCGGATACTAAAATTACACATCTTAATCTTGGATACAACTGTATAGACGCTGCAG	GAATCATAGCACTTGCGAAGATATTGCCG
GATACTAAAATTACACATCTTAGTCTTGAGTTTAATAATGTAGACAATATTGGTGCAATAG	CACTTGCGAAGATATTGCCGG
ATACTAAGATTACATATCTTAATCTTGAGGGTAATG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP012421.1\|WP_013006747.1\|3999_4434_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|276809
NZ_CP012421.1\|WP_053351156.1\|4631_5117_+\|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl\|CDD\|379188
NZ_CP012421.1\|WP_013006750.1\|5521_6163_-\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|222854
NZ_CP012421.1\|WP_082262808.1\|3261_3882_-\|tetratricopeptide-repeat-protein	unknown	unknown	gnl\|CDD\|276809
NZ_CP012421.1\|WP_013006751.1\|6384_6564_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP012421.1\|WP_053351157.1\|6728_9104_+\|chromosomal-replication-initiator-protein-DnaA	unknown	unknown	gnl\|CDD\|371642
NZ_CP012421.1\|WP_014392852.1\|5279_5510_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP012421.1\|WP_053351158.1\|10448_11681_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP012421.1\|WP_053351159.1\|12135_12357_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP012421.1\|WP_053351160.1\|13407_13767_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP012421.1\|WP_013006747.1\|3999_4434_-\|hypothetical-protein	gnl\|CDD\|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	0.000134664
NZ_CP012421.1\|WP_053351156.1\|4631_5117_+\|helix-turn-helix-domain-containing-protein	gnl\|CDD\|379188	pfam13443, HTH_26, Cro/C1-type HTH DNA-binding domain. This is a helix-turn-helix domain that probably binds to DNA.	0.00149187
NZ_CP012421.1\|WP_013006750.1\|5521_6163_-\|AAA-family-ATPase	gnl\|CDD\|222854	PHA02518, PHA02518, ParA-like protein; Provisional.	6.43652e-56
NZ_CP012421.1\|WP_082262808.1\|3261_3882_-\|tetratricopeptide-repeat-protein	gnl\|CDD\|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	1.36211e-06
NZ_CP012421.1\|WP_053351157.1\|6728_9104_+\|chromosomal-replication-initiator-protein-DnaA	gnl\|CDD\|371642	pfam11638, DnaA_N, DnaA N-terminal domain. This family of proteins represents the N-terminal domain of DnaA, a protein involved in the initiation of bacterial chromosomal replication. The structure of this domain is known. It is also found in three copies in some proteins. The exact function of this domain is uncertain but it has been suggested to play a role in oligomerization.	8.33785e-11

>NZ_CP012421.1|WP_082262808.1|3261_3882_-|tetratricopeptide-repeat-protein
MRVLDKLERKIIKKLQRYKIDGVGKISATNHKQRSPKGKILASAEQEKWGRYFELGMYWHGREEGNHKELIYLRRCLQLNPENEPANCYVGMILQKQGRYHESLAAYRKAISLRPELKELDSKNARLKKMYAEERLRADKTINDCWSMDFIMHDSLENGRRYRYIMLCSTPLAILYVGWILTNCSLRTLLGTLADGLKCTIKNFRL
>NZ_CP012421.1|WP_013006747.1|3999_4434_-|hypothetical-protein
MIKVTQTARLVHISYDPNVTSFVDQACNADNADHDNQSSSKLPTAKTITVLISFLKLSKYDDEWLRVGKQLLLLQRPTDKRRCYRSVLLCFKSVIKTHSDNYVAYYFSGITLCLLGSYDEAIKMFDMSLRYNKEKSTALVHTFI
>NZ_CP012421.1|WP_053351156.1|4631_5117_+|helix-turn-helix-domain-containing-protein
MDIALRIQKFLSQKIKAYKIQQKDLVLGTSLSRSTISKLLNAILLNPNIITILIIATFFECDIDEVLGRTKFIKNTKKYQLYVSLTLNDINNNLFSFLKTKIQQLNITEYILEKNCRVGSSTITHFINTNSDNRILSIKVIVKLADYFQIAIDEMIGRTKI
>NZ_CP012421.1|WP_014392852.1|5279_5510_-|hypothetical-protein
MTRKRIALPKKPVQKNTTQFIDAWVSGNIKHEQQDQKHKRTTIFLPEELHKKLKIEAARREVTMTEIIIEAVEKSI
>NZ_CP012421.1|WP_013006750.1|5521_6163_-|AAA-family-ATPase
MILSFLNQKGGVGKTTLSINVATCLSLKKQKVLLIDADPQNSSLDWVATRRKENLFTVVGLTKPIIHKEVSKLVKNYDHIIIDGPPRIYDVAKSAIVTSDLVVMPVQPSPYDIWAANEVVSLIKEVSQPLEGIKNIKSAFLINRKISNTAIGRDVEQALDHYGMDILQTHICQRVAYAETAAIGSTVMEKHNRDNLAVREITDLTNEILEKYN
>NZ_CP012421.1|WP_013006751.1|6384_6564_+|hypothetical-protein
MLKKINVKRISLDIPEHIIEKIDKICAENFITRRKWFIDAANDKLEKYKLNKIDTIVKQ
>NZ_CP012421.1|WP_053351157.1|6728_9104_+|chromosomal-replication-initiator-protein-DnaA
MQSFYPLATPSDNVGIHQYYDTTKNQSYVPITGCVLSHILSSKELSTLEKMYYILADGLSCINFSKGHNRSISLPAKSWAERLSCSKSEVFVLQKSLEAKGHFIITRDKNDQGQNKRNIITTTIPDKVFNDLKYEPDRFDLGDIDNTKTRHSKSDKLDSIDNIFVPTIEGKRQHLEKTKMFIRVPYEFLKQLNSNCSISATAKTITLYLFTKIYKSSLSSNNDKGNRDTDSKDKDNRITTETYIDASNSNKNDYKDFYDHAYDHDHSITNNLHSIVTSYQELQKELKLHRNAISRALKDLEDNNLIKRDKLVIRNNDDYNSRADKSLWQISLLNISLINNNDQKKQKEQKQQIVIEYNNNKALEIAKEWKCTEYDPPCTDSGQLYSKDFVSKNSIIKNIDYIDVNLKNLSINDSSNIYKSEENFKKEFLGNEQSSFPSEFYNNSSFLNDSEAGCFVSKEEYHETASLKLPCANIHPEIVARTIKDISENKELRHFYPLSEKDVDILNFKANREFSTNFVNQLLLKLYIKYPEKKFKNKFIFLSYMKKVLASEKHQGPLVNHTTFRFSCNIGAEEQNMLEHEQYLSQIENSFDTSKEMQVKKKIAGRFSTSIAYSILTQVEFKISANNSFITVLIPSNLALSERQIEILSEQLETVYGMNGYYVQKVEEVEDTSNERKRRDEEKVMLYPKIITSDVVSDGTHAGILAKSGGLDTIEANTAWHHIRQGLIEELGEAMYTVWFEKAVVKECNDTSTLKFTMPARFIADWVRNNYSHVIQRLGSSAGVKRVEYGY
>NZ_CP012421.1|WP_053351158.1|10448_11681_-|hypothetical-protein
MNEIFPLLLNTFHTIWGHTNTCADSNKTFDVIKLGNMLRSRKYYLMDQQLISSKCFPTNNCSLDKAVECNIFEFLSKSKNSLGLTAYVEHLNGTIFRVVNSFDNTRIFGAEYKIFSPNNTPLLYEHRCFTDESVLIKNVHKVVLQGHDVSIRTPKTGKCGIFLIEYMSDKLKHMLQEVTPDVVNNTTSSILDFFFTTVTENISLNYTSNYEVIDYNSTNSTDISNYDTQHSQDNTMLVGGLVLASAVSITYLLTHMYNKFCKKTPVAQTETADINIGDPTLMGINSRKAKIYYSDSSSADGAEYAIVNISSKRAQYTKATYDTSNPSSSHSDDHERMELMSSTDSSIQDSSNVNSHRLAKLHIEGRDSGIPIYDNCEPVYDHPRLPPRPITVIEQHHDDASLLGLSELLC
>NZ_CP012421.1|WP_053351159.1|12135_12357_-|hypothetical-protein
MTVTVKLSASNDKSQEGKVSKEQIKAAKAFALAAAQKEALKAKLAGWREESKQILKLLQTKYSLCFPIPAKPL
>NZ_CP012421.1|WP_053351160.1|13407_13767_+|hypothetical-protein
MHNMIVYAQKHGILKDHEILNQFNSSDGHNVLKNLSTTCEKHYMEHHGENIKQLLDDEHVMVGAKKYSCPLKYMKHERQNNIPSFVDEKHIASAFAKVKAKVKELRLEKELEHTMTMRL

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP012421_1	1.1\|2626\|42\|NZ_CP012421\|CRISPRCasFinder	2626-2667	42	NZ_CP012421	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence	2626-2667	0	1.0
NZ_CP012421_1	1.1\|2626\|42\|NZ_CP012421\|CRISPRCasFinder	2626-2667	42	NC_017029	Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence	4096-4137	0	1.0
NZ_CP012421_1	1.1\|2626\|42\|NZ_CP012421\|CRISPRCasFinder	2626-2667	42	NC_013938	Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence	2788-2829	0	1.0
NZ_CP012421_1	1.1\|2626\|42\|NZ_CP012421\|CRISPRCasFinder	2626-2667	42	NZ_CP015014	Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence	3215-3256	0	1.0
NZ_CP012421_1	1.2\|2707\|42\|NZ_CP012421\|CRISPRCasFinder	2707-2748	42	NZ_CP012421	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence	2707-2748	0	1.0
NZ_CP012421_1	1.3\|2663\|29\|NZ_CP012421\|PILER-CR	2663-2691	29	NZ_CP012421	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence	2639-2667	0	1.0
NZ_CP012421_1	1.3\|2663\|29\|NZ_CP012421\|PILER-CR	2663-2691	29	NC_017029	Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence	4109-4137	0	1.0
NZ_CP012421_1	1.3\|2663\|29\|NZ_CP012421\|PILER-CR	2663-2691	29	NC_013938	Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence	2801-2829	0	1.0
NZ_CP012421_1	1.3\|2663\|29\|NZ_CP012421\|PILER-CR	2663-2691	29	NZ_CP015014	Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence	3228-3256	0	1.0
NZ_CP012421_1	1.4\|2753\|21\|NZ_CP012421\|PILER-CR	2753-2773	21	NZ_CP012421	Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence	2728-2748	0	1.0
NZ_CP012421_1	1.1\|2626\|42\|NZ_CP012421\|CRISPRCasFinder	2626-2667	42	NC_009897	Rickettsia massiliae MTU5 plasmid pRMA, complete sequence	3573-3614	1	0.976
NZ_CP012421_1	1.3\|2663\|29\|NZ_CP012421\|PILER-CR	2663-2691	29	NC_009897	Rickettsia massiliae MTU5 plasmid pRMA, complete sequence	3573-3601	1	0.966
NZ_CP012421_1	1.4\|2753\|21\|NZ_CP012421\|PILER-CR	2753-2773	21	NC_009897	Rickettsia massiliae MTU5 plasmid pRMA, complete sequence	3492-3512	1	0.952
NZ_CP012421_1	1.2\|2707\|42\|NZ_CP012421\|CRISPRCasFinder	2707-2748	42	NC_017029	Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence	4177-4218	2	0.952
NZ_CP012421_1	1.2\|2707\|42\|NZ_CP012421\|CRISPRCasFinder	2707-2748	42	NC_013938	Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence	2869-2910	2	0.952
NZ_CP012421_1	1.2\|2707\|42\|NZ_CP012421\|CRISPRCasFinder	2707-2748	42	NZ_CP015014	Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence	3296-3337	2	0.952
NZ_CP012421_1	1.2\|2707\|42\|NZ_CP012421\|CRISPRCasFinder	2707-2748	42	NC_009897	Rickettsia massiliae MTU5 plasmid pRMA, complete sequence	3492-3533	3	0.929

1. spacer 1.1|2626|42|NZ_CP012421|CRISPRCasFinder matches to NZ_CP012421 (Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence) position: , mismatch: 0, identity: 1.0

catcttaatcttggatacaactgtatagacgctgcaggaatc	CRISPR spacer
catcttaatcttggatacaactgtatagacgctgcaggaatc	Protospacer
******************************************

2. spacer 1.1|2626|42|NZ_CP012421|CRISPRCasFinder matches to NC_017029 (Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence) position: , mismatch: 0, identity: 1.0

catcttaatcttggatacaactgtatagacgctgcaggaatc	CRISPR spacer
catcttaatcttggatacaactgtatagacgctgcaggaatc	Protospacer
******************************************

3. spacer 1.1|2626|42|NZ_CP012421|CRISPRCasFinder matches to NC_013938 (Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence) position: , mismatch: 0, identity: 1.0

catcttaatcttggatacaactgtatagacgctgcaggaatc	CRISPR spacer
catcttaatcttggatacaactgtatagacgctgcaggaatc	Protospacer
******************************************

4. spacer 1.1|2626|42|NZ_CP012421|CRISPRCasFinder matches to NZ_CP015014 (Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence) position: , mismatch: 0, identity: 1.0

catcttaatcttggatacaactgtatagacgctgcaggaatc	CRISPR spacer
catcttaatcttggatacaactgtatagacgctgcaggaatc	Protospacer
******************************************

5. spacer 1.2|2707|42|NZ_CP012421|CRISPRCasFinder matches to NZ_CP012421 (Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence) position: , mismatch: 0, identity: 1.0

catcttagtcttgagtttaataatgtagacaatattggtgca	CRISPR spacer
catcttagtcttgagtttaataatgtagacaatattggtgca	Protospacer
******************************************

6. spacer 1.3|2663|29|NZ_CP012421|PILER-CR matches to NZ_CP012421 (Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence) position: , mismatch: 0, identity: 1.0

gatacaactgtatagacgctgcaggaatc	CRISPR spacer
gatacaactgtatagacgctgcaggaatc	Protospacer
*****************************

7. spacer 1.3|2663|29|NZ_CP012421|PILER-CR matches to NC_017029 (Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence) position: , mismatch: 0, identity: 1.0

gatacaactgtatagacgctgcaggaatc	CRISPR spacer
gatacaactgtatagacgctgcaggaatc	Protospacer
*****************************

8. spacer 1.3|2663|29|NZ_CP012421|PILER-CR matches to NC_013938 (Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence) position: , mismatch: 0, identity: 1.0

gatacaactgtatagacgctgcaggaatc	CRISPR spacer
gatacaactgtatagacgctgcaggaatc	Protospacer
*****************************

9. spacer 1.3|2663|29|NZ_CP012421|PILER-CR matches to NZ_CP015014 (Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence) position: , mismatch: 0, identity: 1.0

gatacaactgtatagacgctgcaggaatc	CRISPR spacer
gatacaactgtatagacgctgcaggaatc	Protospacer
*****************************

10. spacer 1.4|2753|21|NZ_CP012421|PILER-CR matches to NZ_CP012421 (Rickettsia amblyommatis strain Ac37 plasmid pRAMAC18, complete sequence) position: , mismatch: 0, identity: 1.0

aatgtagacaatattggtgca	CRISPR spacer
aatgtagacaatattggtgca	Protospacer
*********************

11. spacer 1.1|2626|42|NZ_CP012421|CRISPRCasFinder matches to NC_009897 (Rickettsia massiliae MTU5 plasmid pRMA, complete sequence) position: , mismatch: 1, identity: 0.976

catcttaatcttggatacaactgtatagacgctgcaggaatc	CRISPR spacer
catcttaatcttggatacaacagtatagacgctgcaggaatc	Protospacer
********************* ********************

12. spacer 1.3|2663|29|NZ_CP012421|PILER-CR matches to NC_009897 (Rickettsia massiliae MTU5 plasmid pRMA, complete sequence) position: , mismatch: 1, identity: 0.966

gatacaactgtatagacgctgcaggaatc	CRISPR spacer
gatacaacagtatagacgctgcaggaatc	Protospacer
******** ********************

13. spacer 1.4|2753|21|NZ_CP012421|PILER-CR matches to NC_009897 (Rickettsia massiliae MTU5 plasmid pRMA, complete sequence) position: , mismatch: 1, identity: 0.952

aatgtagacaatattggtgca	CRISPR spacer
aatgtagacaatactggtgca	Protospacer
*************.*******

14. spacer 1.2|2707|42|NZ_CP012421|CRISPRCasFinder matches to NC_017029 (Rickettsia amblyommatis str. GAT-30V plasmid pMCE_2, complete sequence) position: , mismatch: 2, identity: 0.952

catcttagtcttgagtttaataatgtagacaatattggtgca	CRISPR spacer
catcttagtcttgagtttaataatgtagacaatgctggtgca	Protospacer
*********************************..*******

15. spacer 1.2|2707|42|NZ_CP012421|CRISPRCasFinder matches to NC_013938 (Candidatus Rickettsia amblyommii AaR/SC plasmid pRAM18, complete sequence) position: , mismatch: 2, identity: 0.952

catcttagtcttgagtttaataatgtagacaatattggtgca	CRISPR spacer
catcttagtcttgagtttaataatgtagacaatgctggtgca	Protospacer
*********************************..*******

16. spacer 1.2|2707|42|NZ_CP012421|CRISPRCasFinder matches to NZ_CP015014 (Rickettsia amblyommatis isolate An13 plasmid unnamed_3, complete sequence) position: , mismatch: 2, identity: 0.952

catcttagtcttgagtttaataatgtagacaatattggtgca	CRISPR spacer
catcttagtcttgagtttaataatgtagacaatgctggtgca	Protospacer
*********************************..*******

17. spacer 1.2|2707|42|NZ_CP012421|CRISPRCasFinder matches to NC_009897 (Rickettsia massiliae MTU5 plasmid pRMA, complete sequence) position: , mismatch: 3, identity: 0.929

catcttagtcttgagtttaataatgtagacaatattggtgca	CRISPR spacer
tatcttaatcttgagtttaataatgtagacaatactggtgca	Protospacer
.******.**************************.*******

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

352710 : 363800

Moumouvirus(14.29%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014391919.1\|352710_354498_-	Fe-S protein assembly chaperone HscA	A0A2P1ELQ7	Moumouvirus	9.8e-73	34.6
WP_053350924.1\|354488_354989_-	co-chaperone HscB	F2Y1Q1	Organic_Lake_phycodnavirus	9.3e-05	32.7
WP_045800202.1\|355055_355637_+	ribonuclease HII	G9E3X7	Emiliania_huxleyi_virus	3.8e-26	43.9
WP_045800203.1\|355795_357781_-	excinuclease ABC subunit UvrB	A7WKH8	Acidianus_filamentous_virus	5.7e-05	27.7
WP_014391923.1\|357851_358160_+	glutaredoxin 3	V9QKN6	Rhizobium_phage	1.8e-11	43.4
WP_097920919.1\|358291_360091_+	ATP-binding cassette domain-containing protein	W8CYL7	Bacillus_phage	3.5e-46	29.3
WP_014391926.1\|361103_363800_+	DNA topoisomerase (ATP-hydrolyzing) subunit A	G3M9Z5	Bacillus_virus	9.5e-96	34.0

DBSCAN-SWA_2

933194 : 1008913

Erysipelothrix_phage(16.67%)

transposase,tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_053351047.1\|933194_934187_+\|tRNA	tryptophan--tRNA ligase	NA	NA	NA	NA
WP_082066713.1\|934396_934816_-	BrnT family toxin	NA	NA	NA	NA
WP_053351048.1\|934987_936139_-	ATP-binding protein	NA	NA	NA	NA
WP_045800397.1\|936179_937256_-	hydroxymethylbilane synthase	NA	NA	NA	NA
WP_045799445.1\|941279_941585_-	hypothetical protein	NA	NA	NA	NA
WP_014392459.1\|941901_942915_-	folate-binding protein YgfZ	NA	NA	NA	NA
WP_014392460.1\|943027_943819_+	RMD1 family protein	NA	NA	NA	NA
WP_045800398.1\|943828_944674_+	ribonuclease D	NA	NA	NA	NA
WP_053351049.1\|945070_945592_-	5-formyltetrahydrofolate cyclo-ligase	NA	NA	NA	NA
WP_144010772.1\|945685_945760_-	palindromic element RPE4 domain-containing protein	NA	NA	NA	NA
WP_053351050.1\|945884_947264_-	dihydrolipoyl dehydrogenase	A0A2K5B2C5	Erysipelothrix_phage	2.9e-48	28.8
WP_082066714.1\|947420_948224_-	methyltransferase domain-containing protein	NA	NA	NA	NA
WP_041860612.1\|948171_948432_-	hypothetical protein	NA	NA	NA	NA
WP_053351051.1\|948847_949087_-	hypothetical protein	NA	NA	NA	NA
WP_157752275.1\|950018_950402_-\|transposase	transposase	NA	NA	NA	NA
WP_014392472.1\|950882_951368_-	DUF2532 domain-containing protein	NA	NA	NA	NA
WP_045800404.1\|951372_952215_-	transglycosylase SLT domain-containing protein	NA	NA	NA	NA
WP_014392474.1\|952315_952792_-	hypothetical protein	NA	NA	NA	NA
WP_045800405.1\|953090_953399_-	hypothetical protein	NA	NA	NA	NA
WP_014120844.1\|953699_953825_+	50S ribosomal protein L36	NA	NA	NA	NA
WP_081120948.1\|953821_953965_-	palindromic element RPE5 domain-containing protein	NA	NA	NA	NA
WP_053351052.1\|954036_954798_+	YebC/PmpR family DNA-binding transcriptional regulator	NA	NA	NA	NA
WP_014392477.1\|954794_955541_+	NTP transferase domain-containing protein	A0A2K9L277	Tupanvirus	1.2e-29	36.3
WP_045800407.1\|956017_956251_-	CopG family transcriptional regulator	NA	NA	NA	NA
WP_156132686.1\|957463_957790_+	hypothetical protein	NA	NA	NA	NA
WP_014392485.1\|958489_958894_-	nucleotide pyrophosphohydrolase	NA	NA	NA	NA
WP_014392486.1\|958926_959214_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_082262790.1\|960934_961231_+	acetyltransferase	NA	NA	NA	NA
WP_014392492.1\|961243_962272_-	type 2 isopentenyl-diphosphate Delta-isomerase	NA	NA	NA	NA
WP_014392493.1\|962485_962731_-	hypothetical protein	NA	NA	NA	NA
WP_014392497.1\|964942_965593_+	conjugal transfer protein TraW	NA	NA	NA	NA
WP_014392498.1\|965656_965845_+	type II toxin-antitoxin system prevent-host-death family antitoxin	NA	NA	NA	NA
WP_041860619.1\|966826_967240_+	type-F conjugative transfer system pilin assembly protein TrbC	NA	NA	NA	NA
WP_045799448.1\|969030_969906_+	conjugal transfer protein TraF	NA	NA	NA	NA
WP_157752276.1\|971565_971721_+	hypothetical protein	NA	NA	NA	NA
WP_082262791.1\|972078_972276_+	conjugal transfer protein TraD	NA	NA	NA	NA
WP_157752277.1\|972822_981462_-	hypothetical protein	NA	NA	NA	NA
WP_014392505.1\|981487_983887_-	hypothetical protein	NA	NA	NA	NA
WP_053351056.1\|984580_985030_-	SDR family oxidoreductase	NA	NA	NA	NA
WP_014392507.1\|985118_985295_-	short-chain dehydrogenase	NA	NA	NA	NA
WP_081120751.1\|986924_987221_+\|transposase	transposase	NA	NA	NA	NA
WP_014392509.1\|987464_987785_+	hypothetical protein	NA	NA	NA	NA
WP_045800412.1\|987756_988080_+	hypothetical protein	NA	NA	NA	NA
WP_053351057.1\|990000_990606_+\|transposase	IS256 family transposase	NA	NA	NA	NA
WP_014392515.1\|991160_991766_-	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	A0A223W000	Agrobacterium_phage	4.2e-60	62.0
WP_045800414.1\|991922_993629_-	30S ribosomal protein S1	NA	NA	NA	NA
WP_014392517.1\|993765_994425_-	(d)CMP kinase	NA	NA	NA	NA
WP_053351058.1\|995475_997176_+	hypothetical protein	NA	NA	NA	NA
WP_053351059.1\|997177_997990_-	Dam family site-specific DNA-(adenine-N6)-methyltransferase	A0A1S5PRR3	Streptococcus_phage	2.4e-34	34.2
WP_053351060.1\|998375_999326_+	HAMP domain-containing histidine kinase	B5LWA6	Feldmannia_species_virus	9.3e-06	28.6
WP_053351061.1\|999476_1000205_+	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	NA	NA	NA	NA
WP_053351062.1\|1001168_1002161_+\|transposase	IS110 family transposase	NA	NA	NA	NA
WP_053351063.1\|1002490_1005442_-	hypothetical protein	NA	NA	NA	NA
WP_053351064.1\|1005558_1006263_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_053351065.1\|1006358_1006973_+	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	A0A1B1IUF0	uncultured_Mediterranean_phage	4.0e-10	34.3
WP_157752278.1\|1007560_1008460_-\|transposase	IS4 family transposase	NA	NA	NA	NA
WP_082262793.1\|1008454_1008913_+\|transposase	transposase	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP012421

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP012420

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection