CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP035175	Lactobacillus plantarum strain SRCM103426 plasmid unnamed1	0 crisprs	NA	0	0	1	0
CP035178	Lactobacillus plantarum strain SRCM103426 plasmid unnamed4	0 crisprs	NA	0	0	0	0
CP035176	Lactobacillus plantarum strain SRCM103426 plasmid unnamed2	0 crisprs	NA	0	0	0	0
CP035174	Lactobacillus plantarum strain SRCM103426 chromosome, complete genome	1 crisprs	csa3,DinG,cas3,DEDDh	1	1	8	2
CP035177	Lactobacillus plantarum strain SRCM103426 plasmid unnamed3	0 crisprs	NA	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

21312 : 75636

Streptococcus_phage(27.27%)

protease,transposase,holin

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS31360.1\|21312_22242_-\|transposase	IS30 family transposase	H7BW47	unidentified_phage	2.6e-24	33.7
QAS31361.1\|22268_22409_+	ABC transporter ATP-binding protein	NA	NA	NA	NA
QAS31362.1\|22425_23817_+	MATE family efflux transporter	NA	NA	NA	NA
QAS31363.1\|26251_27322_+	hypothetical protein	NA	NA	NA	NA
QAS31364.1\|27620_27839_+	hypothetical protein	NA	NA	NA	NA
QAS31365.1\|27839_29651_+	LtrC	NA	NA	NA	NA
QAS31500.1\|29717_30056_+	ribonuclease G	NA	NA	NA	NA
QAS31366.1\|30118_30517_-	hypothetical protein	NA	NA	NA	NA
QAS31367.1\|30509_30830_-	acetyl-CoA carboxylase	NA	NA	NA	NA
QAS31368.1\|30822_32124_-	Y-family DNA polymerase	M1Q231	Streptococcus_phage	7.6e-83	40.0
QAS31369.1\|32237_32546_+	hypothetical protein	NA	NA	NA	NA
QAS31501.1\|32980_33190_-	hypothetical protein	NA	NA	NA	NA
QAS31370.1\|33339_34107_-	ParA family protein	F0PIG8	Enterococcus_phage	6.2e-16	30.7
QAS31371.1\|35043_36576_+	plasmid replication protein	NA	NA	NA	NA
QAS31372.1\|36894_39462_+	hypothetical protein	NA	NA	NA	NA
QAS31373.1\|39727_40111_+	hypothetical protein	NA	NA	NA	NA
QAS31374.1\|40082_40742_+	hypothetical protein	NA	NA	NA	NA
QAS31375.1\|40757_41006_+	hypothetical protein	NA	NA	NA	NA
QAS31376.1\|41033_42710_+	ATPase	NA	NA	NA	NA
QAS31377.1\|42712_42919_+	hypothetical protein	NA	NA	NA	NA
QAS31378.1\|42919_44083_+	CHAP domain-containing protein	A0A0A0RSI6	Bacillus_phage	4.5e-10	35.5
QAS31379.1\|44095_44725_+	hypothetical protein	NA	NA	NA	NA
QAS31380.1\|45541_46501_+	DUF3102 domain-containing protein	A0A2H4JAH5	uncultured_Caudovirales_phage	1.7e-34	48.2
QAS31502.1\|46500_46713_+	hypothetical protein	NA	NA	NA	NA
QAS31381.1\|46714_46933_+	hypothetical protein	NA	NA	NA	NA
QAS31382.1\|46929_47229_+	hypothetical protein	NA	NA	NA	NA
QAS31383.1\|47225_47651_+	thioredoxin	NA	NA	NA	NA
QAS31384.1\|47669_48578_+	hypothetical protein	NA	NA	NA	NA
QAS31385.1\|48903_49194_+	hypothetical protein	NA	NA	NA	NA
QAS31386.1\|49195_49696_+	hypothetical protein	NA	NA	NA	NA
QAS31387.1\|49713_50181_+	hypothetical protein	NA	NA	NA	NA
QAS31388.1\|50164_52342_+	hypothetical protein	NA	NA	NA	NA
QAS31389.1\|52343_52979_+\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
QAS31390.1\|53259_53364_+\|holin	putative holin-like toxin	NA	NA	NA	NA
QAS31391.1\|53931_56094_+	type IA DNA topoisomerase	A0A1X9I6W8	Streptococcus_phage	2.2e-103	46.5
QAS31392.1\|56173_57094_-\|transposase	IS30-like element ISLsa1 family transposase	Q9MBM9	Staphylococcus_prophage	1.6e-50	37.9
QAS31393.1\|57287_57554_+	hypothetical protein	NA	NA	NA	NA
QAS31394.1\|57776_59903_+	muramidase	A0A141HSE6	Bacillus_phage	4.4e-11	28.4
QAS31395.1\|59972_60938_+	DUF3991 domain-containing protein	NA	NA	NA	NA
QAS31396.1\|60934_61129_+	hypothetical protein	NA	NA	NA	NA
QAS31397.1\|61131_61752_+	hypothetical protein	NA	NA	NA	NA
QAS31398.1\|61748_63533_+\|protease	ATP-dependent Clp protease ATP-binding subunit	A0A223W0B1	Agrobacterium_phage	1.7e-80	33.7
QAS31399.1\|63598_63913_+	hypothetical protein	NA	NA	NA	NA
QAS31400.1\|64006_66112_+	hypothetical protein	NA	NA	NA	NA
QAS31401.1\|66121_66901_+	class A sortase	NA	NA	NA	NA
QAS31402.1\|69992_70289_+	hypothetical protein	NA	NA	NA	NA
QAS31403.1\|70390_71314_-	GIY-YIG nuclease family protein	NA	NA	NA	NA
QAS31404.1\|71582_72209_+	LtrC-like protein	NA	NA	NA	NA
QAS31405.1\|72221_73181_+	D-2-hydroxyacid dehydrogenase	A0A2R8FCS0	Cedratvirus	2.2e-26	29.3
QAS31406.1\|73298_73685_-	hypothetical protein	NA	NA	NA	NA
QAS31407.1\|73677_73998_-	acetyl-CoA carboxylase	NA	NA	NA	NA
QAS31408.1\|73990_75292_-	Y-family DNA polymerase	M1Q231	Streptococcus_phage	2.1e-80	39.3
QAS31409.1\|75531_75636_-\|holin	putative holin-like toxin	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP035174

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CRISPR-Cas detection and classification

Crispr_ID: CP035174_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP035174_1

360255-360716

Orphan

Consensus_repeat	Method
AAGTCNNANAAGAANAAN	CRT

12 spacers

The CRISPR arrays of CP035174_1

>merge|CP035174|1|360255-360716|CRT
AAGTCGCACAAGAAGTCACACAAGAAGAAAGCGGCCAAGAAACATAAGAAGTCGCACAAGAAGAAAGCGGCCAAGAAACATAAGAAGCCGCACAAGAAGAAGTCTTCTAAGAAGCATGGCAAGTCGCACAAGAAAAAATCTTCGAAGAAGCACGGCAAGTCCAATAAGAAGAAGTCTTCGAAGAAGCACGGCAAGTCCAATAAGAAAAAGTCTTCCAAGAAACACGGCAAGTCGCACAAGAAGAAGTCTTCGAAGAAGCACGGCAAGTCCAATAAGAAAAAGTCTTCCAAGAAACATGGCAAGTCCAGTAAGAAGAAGTCTTCCAAGAAACATGGCAAGTCGCACAAGAAGAAATCTTCCAAGAAGCACGGCAAGTCCAATAAGAAGAAATCTTCCAAGAAGCATGGTAAATCCAGTAAGAAGAAATCATCTAAGAAGCACGGCAAGTCTAACAAGAAAAAA

>CP035174|1|1|360255-360716|CRT
AAGTCGCACAAGAAGTCA	CACAAGAAGAAAGCGGCCAAGAAACATAAG
AAGTCGCACAAGAAGAAA	GCGGCCAAGAAACATAAG
AAGCCGCACAAGAAGAAG	TCTTCTAAGAAGCATGGC
AAGTCGCACAAGAAAAAA	TCTTCGAAGAAGCACGGC
AAGTCCAATAAGAAGAAG	TCTTCGAAGAAGCACGGC
AAGTCCAATAAGAAAAAG	TCTTCCAAGAAACACGGC
AAGTCGCACAAGAAGAAG	TCTTCGAAGAAGCACGGC
AAGTCCAATAAGAAAAAG	TCTTCCAAGAAACATGGC
AAGTCCAGTAAGAAGAAG	TCTTCCAAGAAACATGGC
AAGTCGCACAAGAAGAAA	TCTTCCAAGAAGCACGGC
AAGTCCAATAAGAAGAAA	TCTTCCAAGAAGCATGGT
AAATCCAGTAAGAAGAAA	TCATCTAAGAAGCACGGC
AAGTCTAACAAGAAAAAA

Protein	Signature genes	Signature genes Name	Protein_function
CP035174.1\|QAS28823.1\|367884_368658_-\|CPBP-family-intramembrane-metalloprotease	unknown	unknown	gnl\|CDD\|376806
CP035174.1\|QAS28817.1\|361726_362119_+\|hypothetical-protein	unknown	unknown	unknown
CP035174.1\|QAS28813.1\|349760_351092_+\|NAD(P)/FAD-dependent-oxidoreductase	unknown	unknown	gnl\|CDD\|224169
CP035174.1\|QAS28824.1\|368756_368915_-\|two-peptide-bacteriocin-plantaricin-EF-subunit-PlnF	unknown	unknown	unknown
CP035174.1\|QAS28812.1\|348711_349683_+\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224050
CP035174.1\|QAS28816.1\|354933_355656_+\|aquaporin-family-protein	unknown	unknown	gnl\|CDD\|223653
CP035174.1\|QAS28811.1\|347184_348711_+\|ABC-transporter-permease/substrate-binding-protein	unknown	unknown	gnl\|CDD\|270328
CP035174.1\|QAS28820.1\|364166_364445_+\|hypothetical-protein	unknown	unknown	unknown
CP035174.1\|QAS28819.1\|363801_364065_+\|hypothetical-protein	unknown	unknown	unknown
CP035174.1\|QAS28821.1\|364706_365482_-\|IS5-like-element-ISLpl3-family-transposase	unknown	unknown	gnl\|CDD\|379129
CP035174.1\|QAS28808.1\|343679_344600_-\|ribonucleoside-hydrolase-RihC	unknown	unknown	gnl\|CDD\|182713
CP035174.1\|QAS28807.1\|342941_343514_+\|acetyl-CoA-carboxylase-biotin-carboxyl-carrier-protein-subunit	unknown	unknown	gnl\|CDD\|235777
CP035174.1\|QAS28825.1\|368939_369110_-\|two-peptide-bacteriocin-plantaricin-EF-subunit-PlnE	unknown	unknown	unknown
CP035174.1\|QAS28809.1\|344736_345648_+\|oxidoreductase	unknown	unknown	gnl\|CDD\|180448
CP035174.1\|QAS28815.1\|353089_354919_+\|type-1-glycerol-3-phosphate-oxidase	unknown	unknown	gnl\|CDD\|380303
CP035174.1\|QAS28814.1\|351557_353075_+\|glycerol-kinase	unknown	unknown	gnl\|CDD\|234594
CP035174.1\|QAS28818.1\|362334_362655_+\|hypothetical-protein	unknown	unknown	unknown
CP035174.1\|QAS28826.1\|371542_372919_+\|HlyD-family-secretion-protein	unknown	unknown	gnl\|CDD\|273387
CP035174.1\|QAS28810.1\|346500_346947_-\|ribonuclease-H	unknown	unknown	gnl\|CDD\|178927
CP035174.1\|QAS28822.1\|366244_367243_+\|IS30-family-transposase	unknown	unknown	gnl\|CDD\|225382

Protein	Function_ID	Function_description	E-value
CP035174.1\|QAS28823.1\|367884_368658_-\|CPBP-family-intramembrane-metalloprotease	gnl\|CDD\|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	8.72071e-10
CP035174.1\|QAS28826.1\|371542_372919_+\|HlyD-family-secretion-protein	gnl\|CDD\|273387	TIGR01000, Mesentericin_Y105_secretion_protein_MesE, bacteriocin secretion accessory protein. This family represents an accessory protein that works with the bacteriocin maturation and ABC transport secretion protein described by TIGR01193. [Transport and binding proteins, Other].	0
CP035174.1\|QAS28813.1\|349760_351092_+\|NAD(P)/FAD-dependent-oxidoreductase	gnl\|CDD\|224169	COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes [Energy production and conversion].	6.51978e-123
CP035174.1\|QAS28812.1\|348711_349683_+\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224050	COG1125, OpuBA, ABC-type proline/glycine betaine transport systems, ATPase components [Amino acid transport and metabolism].	1.08528e-132
CP035174.1\|QAS28816.1\|354933_355656_+\|aquaporin-family-protein	gnl\|CDD\|223653	COG0580, GlpF, Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family) [Carbohydrate transport and metabolism].	4.25252e-82
CP035174.1\|QAS28811.1\|347184_348711_+\|ABC-transporter-permease/substrate-binding-protein	gnl\|CDD\|270328	cd13610, PBP2_ChoS, Substrate-binding domain ChoS of an osmoregulated ABC-type transporter and related proteins; type 2 periplasmic-binding protein fold. Osmoprotectant binding lipoprotein ChoS of Lactococcus lactis is predicted to be involved in uptake of compatible solutes such as choline and glycine betaine, but the relative substrate preference is not known. To counteract the efflux of water, microorganisms accumulate the compatible solutes for a sustained adjustment to high osmolarity surroundings. ChoS belongs to the type 2 periplasmic binding fold protein superfamily (PBP2). The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	1.16862e-155
CP035174.1\|QAS28821.1\|364706_365482_-\|IS5-like-element-ISLpl3-family-transposase	gnl\|CDD\|379129	pfam13340, DUF4096, Putative transposase of IS4/5 family (DUF4096).	1.14622e-32
CP035174.1\|QAS28808.1\|343679_344600_-\|ribonucleoside-hydrolase-RihC	gnl\|CDD\|182713	PRK10768, PRK10768, ribonucleoside hydrolase RihC; Provisional.	0
CP035174.1\|QAS28807.1\|342941_343514_+\|acetyl-CoA-carboxylase-biotin-carboxyl-carrier-protein-subunit	gnl\|CDD\|235777	PRK06302, PRK06302, acetyl-CoA carboxylase biotin carboxyl carrier protein.	3.55302e-28
CP035174.1\|QAS28809.1\|344736_345648_+\|oxidoreductase	gnl\|CDD\|180448	PRK06182, PRK06182, short chain dehydrogenase; Validated.	1.21811e-162
CP035174.1\|QAS28815.1\|353089_354919_+\|type-1-glycerol-3-phosphate-oxidase	gnl\|CDD\|380303	NF033461, glycerol3P_ox_1, type 1 glycerol-3-phosphate oxidase. Glycerol-3-phosphate oxidase, also called alpha-glycerophosphate oxidase (GlpO), is an FAD-dependent enzyme related to the glycerol-3-phosphate dehydrogenase GlpD. Notably, GlpO releases hydrogen peroxide, which can contribute to virulence.	0
CP035174.1\|QAS28814.1\|351557_353075_+\|glycerol-kinase	gnl\|CDD\|234594	PRK00047, glpK, glycerol kinase GlpK.	0
CP035174.1\|QAS28810.1\|346500_346947_-\|ribonuclease-H	gnl\|CDD\|178927	PRK00203, rnhA, ribonuclease H; Reviewed.	0.000124132
CP035174.1\|QAS28822.1\|366244_367243_+\|IS30-family-transposase	gnl\|CDD\|225382	COG2826, Tra8, Transposase and inactivated derivatives, IS30 family [DNA replication, recombination, and repair].	2.24499e-87

>CP035174.1|QAS28816.1|354933_355656_+|aquaporin-family-protein
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYASSFMSPAHLNPAVSLGMAVAGKFPWAYVIPYSTAQIAGGVIGGLVVWLHYYPHWQATKDAGAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKGQFTAGLNPIVVGILIIAIGLSLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP
>CP035174.1|QAS28815.1|353089_354919_+|type-1-glycerol-3-phosphate-oxidase
MAFSATTRQQNIERLQNETLDLLVIGGGITGAGVAIQSTAMNMKTGLIDMQDFAEGTSSRSTKLVHGGIRYLKTFDVGVVADTVKERAVVQGIAPHIPKPDPMLLPIYDETGATFDLFSVKVAMDLYDKLAEVKDPKFTNYTLSREEVLQREPQLNPKHLVGGGVYLDFRNNDARLVIENIKKAHENGGIMASHVKATGLLHDDDGRINGITAEDLLTGASFEIHARIVINTTGPWSDTIRKMDHTEKYQSQMRPTKGVHLVVDAEKLPVPQPTYFDSGDQDGRMIFVVPRENKTYFGTTDTDYHGDLKHPTVTQEDVDYLLEIVNRRFPTAKITLDDIEASWAGLRPLIAANGSSDYNGGNSGKISDKSFKEVIKVVDQFESQEASREDVESVLNDLETSLAEDKLSPSAVSRGSSLTSSPDGLLTLAGGKITDYRKMAAGAMALIAKILADKYDAHFNEINSKNLPVSGGDIDPHNVDATIAFYAKQGTDNGLTNDEALRIANMYGSNAARVFSLIGKVEPTHGLSLAETISLRYALDEEMTLTPVDYLLRRTNHLLFMRDTLDSLKDGVIATMADYLGWDTATVKAQTELLNQTIAESDLTALKQG
>CP035174.1|QAS28814.1|351557_353075_+|glycerol-kinase
MTDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTIANAFIESGIKPAQISGIGITNQRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGYGDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGELLFGTIDTWLLWKLTGGATHVTDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPISGMAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGIRLVKSAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEARGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSGIEIPVLKVDGGAARNDWLMQFQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERTNLYEGWQAAVKATQVFKHTPYHAGK
>CP035174.1|QAS28813.1|349760_351092_+|NAD(P)/FAD-dependent-oxidoreductase
MAEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEARQAAQHLQGQGLIGASKIDWPALMAHKRGYTDGINDGTLNGLKGQDIATLHGQAHFQSDNQLVVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLDLDQMPKRVTFVGGGYVGFELATIANAAGADVHVIHHNDRPLKAFDADLVKDLIAAMTADGITFDLNTDVQAITKTATGLQLTADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVSDTPVPKLTPVAGFEARYLVGELTHPGAAIKYPVVPTQVFAAPKLAQVGISAAAATEHPDEYRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATLLSDVADEMINYFTLLIEKHVTLPDLQRLVLAYPTPASDLQYLY
>CP035174.1|QAS28812.1|348711_349683_+|ABC-transporter-ATP-binding-protein
MTTAIEFQHVQKDFNGQTVIPDLNLTIDQGELFVLVGTSGSGKTTSLKMINCLEPLTAGKILVNGTDTTTIPVRSLRWQMGYVLQQIALFPTMTVAQNIAVIPEMKGTAKKEINQTIDELLAEVGLDPKEYRDRMPSELSGGEQQRIGILRAIAAQPDIVLMDEPFSALDPISRQQLQDLVLRLHARYHNTIVFVTHDMNEALKLGDRIGVMQHGQLIQVDTPAALAQHPVNDFVRNFFGASRAKNVYDVYVGRVGLIQGYLTEKPSVASGRIQSLDVQATLRTAFTALTDHDYVAVTEENRVVGYLDRQRIVAYLSQHEEVS
>CP035174.1|QAS28811.1|347184_348711_+|ABC-transporter-permease/substrate-binding-protein
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSVLQTIPSLALLGLLIPLVGIGTVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGMSRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIGAGGLGTFIMLGIDRNDTSLLLIGAISSALLAILLSAFVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAGKLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLETLVKGNNPAGQTANQTYQLAKQRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISDLTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSMEPALRYEAISKGKINLVDAYATDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMNYEVNVKKQSASTVAHRYLVKHGLLKEGR
>CP035174.1|QAS28810.1|346500_346947_-|ribonuclease-H
MATIHLYLASTNHQSTNITATTPAAWVVILRYADHQKALNDGGYGHTETSLALSALTTALATLKRHDLPVHIHTTTEQVAAVISEQRYLSWRQNNWDVPEADQPDLPQWQTLIPIIEEFPNLTITYHPVDDPVMTTTTTTLTAVMTKL
>CP035174.1|QAS28809.1|344736_345648_+|oxidoreductase
MSQPRVILITGASSGIGKATALKLQAQGNIVYGAARRIEKMTDLAAAGVHVLKVDVTDEATLIAAVKTIQVEQGRIDVLINNAGYGSYGSLEDVPVAEGEYQFKVNVFGVMRLTQLVLPLMRAQHRGRIINVSSIGGKIYQPLSAWYMGTKHAIEGMSDSLRMEVAPFGIDVVIVEPGGIKTEWAGIAEKKLLAVSGETAYHEQAVKVGAMLSLFDQFASNPGVIAKLMVRAVNDVKPKTRYHAGMGAGVSLTARQWLSDRQFDSMMNSAVNGTAKLSKVTRRKDRRTGSRHYRHGKIPADNN
>CP035174.1|QAS28808.1|343679_344600_-|ribonucleoside-hydrolase-RihC
MSTKIIMDTDPGIDDAAAITFALNHPDLDLQLITTVAGNVTVDKTTLNALKLTRFFNSDVPVAGGAAQPLIKPFEDAVRIHGVSGMPGYDFPTDLAKPLPETAVEALRDYIMAAEQPITLVPTGAYTNIALLFKTYPEVMPHIKEIVAMGGALGKGNMTSAAEFNVFTDLHAAEIMYQSGVPITMVGLDVTMKALLTRESIEQLPTLGKTGEMLHALFGHYNDRNQTGVAMHDVNTLFYLLHPEAFTTERMWIDVQTEGPANGETVGDIRGAYHDGKTNATVCVDIDAAAFNKWFLETVAAIDQPK
>CP035174.1|QAS28807.1|342941_343514_+|acetyl-CoA-carboxylase-biotin-carboxyl-carrier-protein-subunit
MELDKIYQLMDKFEQSSLTSFEYRDQDFEIQMGKKGHGKTTVTPQPAPLPNQPVIDPTPVTPTPVSTVSTPVVAEAEPTPVATSTASNPTSVVTPDPEPEPAPIDPKECVTAPVVGIYYPAHSSEEAPYVKLGDHVSVGQQVGLIQAMQMMRPVVAKQAGTVKAFLVKNGEEVNFGQPLIQLVPDTPDDI
>CP035174.1|QAS28817.1|361726_362119_+|hypothetical-protein
MIRVLQVNPPDDPEYRDPFVLLSDGNMQIWVFADEFNNKIGDKYQYPYMTLFPLLEGDVEMQKGKVFAVKALSDIQYSYDLYGQINENWKLKVGEFVIDSGEENYEGFKRGDFVHFRCDRVDLIDFNKFI
>CP035174.1|QAS28818.1|362334_362655_+|hypothetical-protein
MFTKYNDLDLLELFWSESESLTGEIDDGEVLYRREISDFELTLFIYTYEMKCNVFLSYKDEDILSIELDNISELKKNEDQLYFYSNESVKLSVIFGKIFSINFQDK
>CP035174.1|QAS28819.1|363801_364065_+|hypothetical-protein
MATIIIIGVVIIGITLGLGYWSYLLGKRNQSNLVGTIIPVAYFVVRAILAVMTQTTTHGLVSSLVGSLLFSLIYYGVFVWGKHRANK
>CP035174.1|QAS28820.1|364166_364445_+|hypothetical-protein
MLVFIVIGLITAVITIGLGYWSYTLGKRNKSNIVGSVIPAGYFIVRLVFVLVLGEGVRELITSLVGNLVITVIYVLLFVWGRQRFNKQKQTK
>CP035174.1|QAS28821.1|364706_365482_-|IS5-like-element-ISLpl3-family-transposase
MTTPKRYELEDAQWDRIKGYFPPYRTGRPSSLDNRTALNAILWLMRSGAPWRDLPERYGSWKTVYSRFRAWVSSGLFEQVFLELIDDPDMENLSLDSTIVRAHQKATGGKKNAECMVENQAIGLSRGGRTTKIHALVDGLGNPLGFRLTGGQVHDSQVASELLEGFDISQSNIIADKAYGTAKLRQYIEDKAGVYTIPPKENTKDKWTCDYHVYCERHLIENFFNQLKNFRRIATRYDKLAHVYLATVYIASICILLK
>CP035174.1|QAS28822.1|366244_367243_+|IS30-family-transposase
MSPYNHLTLKDRECILLGVTLNDTYQVIAEKIGCSKATVSREIKRNGGRKAYSAVKAQENYQGRRLKSRRPRLLTDLKLRDFVLHCIVQRQWSPEQISGRLVHENSEWHVSYNTIYRGIERDNLGIKRKNHEARGFARKLRHRGKTRKVKGTIKERRGRFNDVPSVHERPVSCENRSWFGHWESDTVRGKTGRSALVTLVDRKSRYLLSQRVPKVNAKNVTQAMIDLLHTVTPKRVRTLTPDRGTEFAGYREVSQELSIPVYFPDPHAPQQRGTNENTNGLIREYFPKGTDLDQLTDQDIDKFVRDLNHRPRKVLGWKSPFEVFFGTKLRLI
>CP035174.1|QAS28823.1|367884_368658_-|CPBP-family-intramembrane-metalloprotease
MPLIKVIITAIYYLIVLFLMNPLTDIMGIKDGPFQFILTESIILIAVIILNRRYVKQPIHWLPVNIMSILRKNSLPLSLAIIFLLIFFRNHMYQFLISLLLSLIVAITEEYAFRGMIFRTLLALNLKKFATLQATIASMMTASLIFAAMHLFNLLSQPVWSVLCQVLYVIGLGILLAAIYLKTGSLLAAISVHWLIDFSSFYSQGIDPTQSPINGPMEALLKGLFLNILFIGIATFILSSKHWKLLSILNIEDKIDE
>CP035174.1|QAS28824.1|368756_368915_-|two-peptide-bacteriocin-plantaricin-EF-subunit-PlnF
MKKFLVLRDRELNAISGGVFHAYSARGVRNNYKSAVGPADWVISAVRGFIHG
>CP035174.1|QAS28825.1|368939_369110_-|two-peptide-bacteriocin-plantaricin-EF-subunit-PlnE
MLQFEKLQYSRLPQKKLAKISGGFNRGGYNFGKSVRHVVDAIGSVAGIRGILKSIR
>CP035174.1|QAS28826.1|371542_372919_+|HlyD-family-secretion-protein
MRKDLLESGEFYGIRFQNFSTLLIIPVTLLLIGTILFSLVAKREIVINGTGTVQPTGTVPVIQATVNSAIKKNYLVEGARVKKGQKLLVYTNVFNRNKLREDNVSKRQLQRQVTALDHFKDGINTDSDVFLTTDEFGYRELLQSYLKQRQIYLTENQMLAAKSSAASTKKATLTKTAQQVVSNTKTSLDAYQALYNAVANGSKYANSAKYGSVFNEYVAKSKEANNANDKKEVKSATLADIQQQIDTLQDSLSSAKVQLEELQDFDDTKYSVETNNTKLAMLKNDQLSEVAEKRMKAEQDLRTVKADIDSLTTQSKSYTIKATKAGVLHVADNYRGAKYVSSGAEIAKILPIIKQQRRVNVKLYISTADISSVKVKQAIRFKVTRNVPKPIILDGVVTKIGVSPVEESKATFYEVTAVVPITADQAQLLRYGMIGQTAIITGKVTWFNYYKDKVLDNN

You can click texts colored in the table to view more detailed information

Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
CP035174_1	1.2\|360321\|18\|CP035174\|CRT	360321-360338	18	CP035174.1	360237-360254	2	0.889

1. spacer 1.2|360321|18|CP035174|CRT matches to position: 360237-360254, mismatch: 2, identity: 0.889

gcggccaagaaacataag	CRISPR spacer
gccgctaagaaacataag	Protospacer
** **.************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP035174_1	1.1\|360273\|30\|CP035174\|CRT	360273-360302	30	NZ_CP006988	Rhizobium sp. IE4771 plasmid pRetIE4771b, complete sequence	31047-31076	7	0.767
CP035174_1	1.1\|360273\|30\|CP035174\|CRT	360273-360302	30	NC_042345	Phage Salvo, complete genome	4721-4750	8	0.733

1. spacer 1.1|360273|30|CP035174|CRT matches to NZ_CP006988 (Rhizobium sp. IE4771 plasmid pRetIE4771b, complete sequence) position: , mismatch: 7, identity: 0.767

cacaagaagaaagcggccaagaaacataag	CRISPR spacer
cacaagaataaagccgccaagaagctccgg	Protospacer
******** ***** ********.* . .*

2. spacer 1.1|360273|30|CP035174|CRT matches to NC_042345 (Phage Salvo, complete genome) position: , mismatch: 8, identity: 0.733

cacaagaagaaagcggccaagaaacataag	CRISPR spacer
ccggggaagtaagcggacaagaaacatact	Protospacer
*  ..**** ****** ***********

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

37665 : 54775

Lactobacillus_phage(30.0%)

tail,portal,capsid,head,terminase,integrase

attL 34236:34249|attR 44364:44377

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
34236:34249	attL	NA	NA	NA	NA
QAS28526.1\|37665_38820_-\|integrase	site-specific integrase	A0A172LN96	Lactococcus_phage	6.1e-60	36.7
QAS28527.1\|38876_39596_-	helix-turn-helix domain-containing protein	Q20DG0	Lactobacillus_phage	2.1e-10	50.6
QAS28528.1\|39687_39876_+	DNA-binding protein	NA	NA	NA	NA
QAS28529.1\|39918_40026_+	hypothetical protein	NA	NA	NA	NA
QAS28530.1\|40141_40396_+	hypothetical protein	NA	NA	NA	NA
QAS28531.1\|40392_41196_+	DNA replication protein	NA	NA	NA	NA
QAS28532.1\|41195_42770_+	hypothetical protein	NA	NA	NA	NA
QAS28533.1\|43031_43388_+	pathogenicity island protein	NA	NA	NA	NA
QAS28534.1\|43387_43579_+	hypothetical protein	NA	NA	NA	NA
QAS28535.1\|43565_43904_+\|head,tail	head-tail adaptor protein	A0A2P0ZLF0	Lactobacillus_phage	3.2e-09	36.6
QAS28536.1\|43896_44286_+	HNH endonuclease	A0A1J0MFT4	Staphylococcus_phage	1.0e-19	45.6
QAS28537.1\|45069_45543_+\|terminase	phage terminase small subunit P27 family	NA	NA	NA	NA
44364:44377	attR	NA	NA	NA	NA
QAS28538.1\|45539_47243_+\|terminase	terminase large subunit	A0A2H4JBN3	uncultured_Caudovirales_phage	2.4e-121	40.5
QAS28539.1\|47196_47397_+	hypothetical protein	NA	NA	NA	NA
QAS28540.1\|47397_48498_+\|portal	phage portal protein	A0A2H4J9Q5	uncultured_Caudovirales_phage	4.6e-49	34.6
QAS28541.1\|48494_50024_+\|capsid	phage major capsid protein	B8R651	Lactobacillus_phage	2.1e-44	26.0
QAS28542.1\|50139_50409_+\|head,tail	phage gp6-like head-tail connector protein	NA	NA	NA	NA
QAS28543.1\|50558_50936_+	hypothetical protein	NA	NA	NA	NA
QAS28544.1\|51059_51260_+	cold-shock protein	Q9AZD3	Lactococcus_phage	1.4e-20	71.4
QAS28545.1\|51346_51577_+	hypothetical protein	NA	NA	NA	NA
QAS28546.1\|52179_52887_+	response regulator transcription factor	W8CYM9	Bacillus_phage	1.3e-44	40.1
QAS28547.1\|52900_54775_+	cell wall metabolism sensor histidine kinase WalK	W8CYF6	Bacillus_phage	5.5e-34	31.8

DBSCAN-SWA_2

324145 : 368915

Staphylococcus_phage(28.57%)

protease,bacteriocin,transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS28789.1\|324145_325798_-\|transposase	IS1182 family transposase	A0ZS58	Staphylococcus_virus	3.5e-93	41.5
QAS28790.1\|325972_327139_-	hypothetical protein	NA	NA	NA	NA
QAS28791.1\|327733_328549_+	ABC transporter permease	NA	NA	NA	NA
QAS28792.1\|328561_329653_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	7.2e-18	29.7
QAS28793.1\|330031_330304_+	hypothetical protein	NA	NA	NA	NA
QAS28794.1\|330530_331073_+	PadR family transcriptional regulator	NA	NA	NA	NA
QAS28795.1\|331069_332515_+	DHA2 family efflux MFS transporter permease subunit	NA	NA	NA	NA
QAS28796.1\|332840_334157_-	ammonium transporter	H8ZJB2	Ostreococcus_tauri_virus	4.3e-33	29.3
QAS28797.1\|334652_335612_+	L-lactate dehydrogenase	NA	NA	NA	NA
QAS28798.1\|335714_335984_+	hypothetical protein	NA	NA	NA	NA
QAS28799.1\|336213_336777_-\|protease	matrixin family metalloprotease	NA	NA	NA	NA
QAS28800.1\|336970_337639_+	ribose 5-phosphate isomerase A	NA	NA	NA	NA
QAS28801.1\|337796_339302_+	copper oxidase	NA	NA	NA	NA
QAS28802.1\|339566_339935_+	DUF1304 domain-containing protein	NA	NA	NA	NA
QAS28803.1\|340047_340557_-	YbhB/YbcL family Raf kinase inhibitor-like protein	NA	NA	NA	NA
QAS28804.1\|340587_341784_-	DUF2075 domain-containing protein	NA	NA	NA	NA
QAS28805.1\|341893_342364_+	transcriptional regulator	NA	NA	NA	NA
QAS28806.1\|342382_342838_+	hypothetical protein	NA	NA	NA	NA
QAS28807.1\|342941_343514_+	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	NA	NA	NA	NA
QAS28808.1\|343679_344600_-	ribonucleoside hydrolase RihC	NA	NA	NA	NA
QAS28809.1\|344736_345648_+	oxidoreductase	NA	NA	NA	NA
QAS28810.1\|346500_346947_-	ribonuclease H	NA	NA	NA	NA
QAS28811.1\|347184_348711_+	ABC transporter permease/substrate-binding protein	NA	NA	NA	NA
QAS28812.1\|348711_349683_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	1.7e-23	31.7
QAS28813.1\|349760_351092_+	NAD(P)/FAD-dependent oxidoreductase	NA	NA	NA	NA
QAS28814.1\|351557_353075_+	glycerol kinase	NA	NA	NA	NA
QAS28815.1\|353089_354919_+	type 1 glycerol-3-phosphate oxidase	NA	NA	NA	NA
QAS28816.1\|354933_355656_+	aquaporin family protein	M1HWZ0	Paramecium_bursaria_Chlorella_virus	4.1e-30	31.9
QAS28817.1\|361726_362119_+	hypothetical protein	NA	NA	NA	NA
QAS28818.1\|362334_362655_+	hypothetical protein	NA	NA	NA	NA
QAS28819.1\|363801_364065_+	hypothetical protein	NA	NA	NA	NA
QAS28820.1\|364166_364445_+	hypothetical protein	NA	NA	NA	NA
QAS28821.1\|364706_365482_-\|transposase	IS5-like element ISLpl3 family transposase	A0A0N9SHJ4	Paenibacillus_phage	2.1e-27	53.2
QAS28822.1\|366244_367243_+\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	2.4e-52	37.6
QAS28823.1\|367884_368658_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
QAS28824.1\|368756_368915_-\|bacteriocin	two-peptide bacteriocin plantaricin EF subunit PlnF	NA	NA	NA	NA

DBSCAN-SWA_3

1018477 : 1025544

Escherichia_phage(50.0%)

integrase,transposase

attL 1022931:1022944|attR 1032279:1032292

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS29372.1\|1018477_1019341_+	glucose-1-phosphate thymidylyltransferase	K7QKA7	Escherichia_phage	4.2e-98	62.1
QAS29373.1\|1019363_1020653_+	hypothetical protein	A0A2K9VCN9	Lactobacillus_phage	4.5e-27	24.1
QAS29374.1\|1020712_1021294_+	dTDP-4-dehydrorhamnose 3,5-epimerase	I7HJC4	Enterobacteria_phage	3.3e-38	45.1
QAS29375.1\|1021303_1022332_+	dTDP-glucose 4,6-dehydratase	A0A1D7XFE8	Escherichia_phage	2.2e-69	42.4
QAS29376.1\|1022401_1023244_+	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	1.9e-34	37.2
1022931:1022944	attL	NA	NA	NA	NA
QAS29377.1\|1024399_1024894_-\|transposase	transposase	NA	NA	NA	NA
QAS29378.1\|1024956_1025544_-\|integrase	integrase	D2XR58	Bacillus_phage	3.6e-32	42.5
1032279:1032292	attR	NA	NA	NA	NA

DBSCAN-SWA_4

1179412 : 1251191

Lactobacillus_phage(89.13%)

tail,portal,transposase,holin,capsid,protease,tRNA,head,terminase,integrase

attL 1181303:1181318|attR 1200639:1200654

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS31275.1\|1179412_1180078_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
QAS29496.1\|1180770_1181250_-	MarR family transcriptional regulator	NA	NA	NA	NA
1181303:1181318	attL	NA	NA	NA	NA
QAS29497.1\|1181363_1181876_-	hypothetical protein	NA	NA	NA	NA
QAS29498.1\|1181990_1182278_+	hypothetical protein	NA	NA	NA	NA
QAS29499.1\|1182339_1184610_+	excinuclease ABC subunit UvrA	A0A1B1IVR3	uncultured_Mediterranean_phage	8.8e-119	31.5
QAS29500.1\|1185251_1186388_-\|integrase	site-specific integrase	E9LUS1	Lactobacillus_phage	1.5e-212	98.9
QAS29501.1\|1186832_1187015_-	hypothetical protein	A0A2P0ZL93	Lactobacillus_phage	4.4e-13	63.2
QAS29502.1\|1187157_1187472_-	hypothetical protein	E9LUS3	Lactobacillus_phage	7.7e-50	100.0
QAS29503.1\|1187464_1187878_-	hypothetical protein	E9LUS4	Lactobacillus_phage	2.8e-71	98.5
QAS29504.1\|1188208_1188409_-	hypothetical protein	E9LUS6	Lactobacillus_phage	3.2e-25	97.0
QAS29505.1\|1188473_1188863_-	ImmA/IrrE family metallo-endopeptidase	D6PSS8	Lactobacillus_phage	4.6e-36	54.3
QAS29506.1\|1188893_1189220_-	XRE family transcriptional regulator	A0A1B0Y2R0	Lactobacillus_phage	6.9e-17	44.1
QAS29507.1\|1189476_1189692_+	hypothetical protein	NA	NA	NA	NA
QAS29508.1\|1189704_1190457_+	phage repressor protein/antirepressor Ant	E9LUT0	Lactobacillus_phage	2.2e-58	96.5
QAS29509.1\|1190641_1190833_-	hypothetical protein	NA	NA	NA	NA
QAS29510.1\|1190925_1191255_+	DUF771 domain-containing protein	E9LUT3	Lactobacillus_phage	5.8e-48	82.6
QAS29511.1\|1191344_1191599_+	hypothetical protein	NA	NA	NA	NA
QAS29512.1\|1191601_1191826_+	hypothetical protein	E9LUT8	Lactobacillus_phage	2.2e-27	82.4
QAS29513.1\|1192020_1192881_+	DUF1351 domain-containing protein	A0A0P0IV40	Lactobacillus_phage	1.2e-36	34.0
QAS29514.1\|1192881_1193544_+	nucleotide-binding protein	E9LUU1	Lactobacillus_phage	2.7e-121	99.5
QAS29515.1\|1193545_1194205_+	DUF669 domain-containing protein	E9LUU2	Lactobacillus_phage	4.5e-92	95.4
QAS29516.1\|1194251_1194944_+	hypothetical protein	E9LUU3	Lactobacillus_phage	4.7e-132	98.7
QAS29517.1\|1195769_1196555_+	ATP-binding protein	E9LUN6	Lactobacillus_phage	3.1e-132	90.8
QAS29518.1\|1196717_1197404_+	AP2 domain-containing protein	A0A060QSU6	Streptococcus_phage	5.3e-11	49.3
QAS29519.1\|1197481_1197790_+	hypothetical protein	E9LUN7	Lactobacillus_phage	3.5e-47	93.1
QAS29520.1\|1197874_1198366_+	methyltransferase domain-containing protein	O03918	Lactobacillus_phage	1.0e-85	91.8
QAS31276.1\|1198607_1198988_+	hypothetical protein	K4I239	Lactobacillus_phage	1.8e-32	53.4
QAS29521.1\|1199499_1199670_+	hypothetical protein	E9LUP4	Lactobacillus_phage	2.9e-19	91.1
QAS29522.1\|1200511_1201387_+	hypothetical protein	NA	NA	NA	NA
1200639:1200654	attR	NA	NA	NA	NA
QAS29523.1\|1201406_1201784_+	hypothetical protein	NA	NA	NA	NA
QAS29524.1\|1201851_1202517_+	hypothetical protein	NA	NA	NA	NA
QAS29525.1\|1202582_1203110_+	HNH endonuclease	E9LUP7	Lactobacillus_phage	9.6e-77	89.7
QAS29526.1\|1203087_1203618_+	endonuclease	U5U4N5	Lactobacillus_phage	8.5e-33	44.3
QAS29527.1\|1203749_1204205_+\|terminase	phage terminase small subunit P27 family	E9LUP8	Lactobacillus_phage	1.0e-79	96.0
QAS29528.1\|1206135_1206330_+	DUF1056 family protein	E9LUQ0	Lactobacillus_phage	1.8e-25	95.3
QAS29529.1\|1206332_1207526_+\|portal	phage portal protein	E9LUQ1	Lactobacillus_phage	3.1e-224	98.7
QAS29530.1\|1207503_1208259_+\|protease	Clp protease ClpP	E9LUQ2	Lactobacillus_phage	1.3e-122	92.4
QAS29531.1\|1208258_1209491_+\|capsid	phage major capsid protein	E9LUQ3	Lactobacillus_phage	3.7e-212	93.4
QAS29532.1\|1209563_1209896_+\|head,tail	phage gp6-like head-tail connector protein	E9LUQ4	Lactobacillus_phage	7.4e-43	80.0
QAS29533.1\|1209885_1210233_+\|head,tail	head-tail adaptor protein	A0A2P0ZLF0	Lactobacillus_phage	7.0e-44	73.9
QAS29534.1\|1210235_1210643_+	HK97 gp10 family phage protein	A0A2P0ZLE6	Lactobacillus_phage	1.8e-62	89.4
QAS29535.1\|1210642_1211023_+	DUF806 family protein	A0A2P0ZLF4	Lactobacillus_phage	1.2e-57	88.9
QAS29536.1\|1211038_1211704_+\|tail	phage tail protein	A0A2P0ZLH1	Lactobacillus_phage	9.5e-106	90.4
QAS29537.1\|1211778_1212153_+	hypothetical protein	A0A2P0ZLH4	Lactobacillus_phage	4.6e-57	94.4
QAS29538.1\|1212197_1212383_+	hypothetical protein	NA	NA	NA	NA
QAS29539.1\|1217412_1219182_+\|tail	phage tail protein	E9LUR2	Lactobacillus_phage	0.0e+00	91.3
QAS29540.1\|1219248_1221606_+	hypothetical protein	E9LUR3	Lactobacillus_phage	0.0e+00	91.5
QAS29541.1\|1221623_1224476_+\|tail	phage tail protein	E9LUR4	Lactobacillus_phage	1.6e-138	53.7
QAS29542.1\|1224468_1224717_+	hypothetical protein	A0A2K9VDF6	Lactobacillus_phage	2.4e-30	95.9
QAS29543.1\|1224865_1225987_+	collagen-like protein	E9LUR7	Lactobacillus_phage	1.7e-54	93.4
QAS29544.1\|1225983_1226229_+	hypothetical protein	A0A0A1ENR9	Lactobacillus_phage	3.6e-18	80.0
QAS29545.1\|1226240_1227413_+	LysM peptidoglycan-binding domain-containing protein	E9LUR8	Lactobacillus_phage	6.6e-195	89.2
QAS29546.1\|1227412_1227676_+\|holin	holin	E9LUR9	Lactobacillus_phage	6.9e-36	94.3
QAS31277.1\|1227689_1228064_+\|holin	holin	A0A2K9VCG4	Lactobacillus_phage	2.4e-18	71.2
QAS29547.1\|1228638_1229064_-	hypothetical protein	NA	NA	NA	NA
QAS29548.1\|1229076_1229196_-	hypothetical protein	NA	NA	NA	NA
QAS29549.1\|1229228_1230299_-	hypothetical protein	NA	NA	NA	NA
QAS31278.1\|1230353_1230869_+	GtrA family protein	NA	NA	NA	NA
QAS29550.1\|1230968_1231328_-	DUF1516 family protein	NA	NA	NA	NA
QAS29551.1\|1231400_1233257_-	bifunctional homocysteine S-methyltransferase/methylenetetrahydrofolate reductase	NA	NA	NA	NA
QAS29552.1\|1233270_1235577_-	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	NA	NA	NA	NA
QAS29553.1\|1236257_1237433_-\|transposase	IS256 family transposase	NA	NA	NA	NA
QAS29554.1\|1237825_1239463_+	hypothetical protein	NA	NA	NA	NA
QAS29555.1\|1239440_1240616_+	sulfate adenylyltransferase	A0A2P1ELS9	Moumouvirus	2.9e-49	31.9
QAS29556.1\|1240618_1241242_+	adenylyl-sulfate kinase	A0A2K9L4R9	Tupanvirus	4.8e-27	40.6
QAS29557.1\|1241231_1242188_+	bifunctional oligoribonuclease/PAP phosphatase NrnA	NA	NA	NA	NA
QAS29558.1\|1242409_1244149_+	aryl-sulfate sulfotransferase	NA	NA	NA	NA
QAS29559.1\|1244581_1246357_+	SLC13 family permease	NA	NA	NA	NA
QAS29560.1\|1246632_1248024_+	MATE family efflux transporter	NA	NA	NA	NA
QAS29561.1\|1248150_1248396_+	DUF2187 domain-containing protein	NA	NA	NA	NA
QAS29562.1\|1248784_1249231_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
QAS29563.1\|1249502_1251191_+\|tRNA	arginine--tRNA ligase	A0A2I2L3K1	Orpheovirus	1.2e-75	32.9

DBSCAN-SWA_5

1277297 : 1291114

Lactobacillus_phage(72.73%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS29584.1\|1277297_1278527_+	hypothetical protein	A0A2P0ZL72	Lactobacillus_phage	1.5e-213	97.2
QAS29585.1\|1278617_1279589_-	peptidase S41	A0A2P0ZL68	Lactobacillus_phage	1.9e-179	97.5
QAS29586.1\|1279774_1280722_-	LysR family transcriptional regulator	A0A2P0ZL89	Lactobacillus_phage	2.9e-177	99.4
QAS29587.1\|1281065_1281680_+	NAD(P)H-dependent oxidoreductase	A0A2P0ZL77	Lactobacillus_phage	3.1e-111	99.0
QAS29588.1\|1281682_1284121_+	flavocytochrome c	A0A2P0ZL82	Lactobacillus_phage	0.0e+00	99.3
QAS29589.1\|1284208_1284769_+	hypothetical protein	A0A2P0ZL75	Lactobacillus_phage	2.2e-100	98.9
QAS29590.1\|1284854_1285823_-\|transposase	IS30-like element ISLpl1 family transposase	H7BW61	unidentified_phage	6.8e-20	29.9
QAS29591.1\|1285794_1286570_-\|transposase	IS5-like element ISLpl3 family transposase	A0A0N9SHJ4	Paenibacillus_phage	2.1e-27	53.2
QAS29592.1\|1286741_1287182_-	nucleoside 2-deoxyribosyltransferase	A0A2P0ZL88	Lactobacillus_phage	4.4e-75	95.9
QAS29593.1\|1287572_1289492_+	hypothetical protein	A0A2P0ZL91	Lactobacillus_phage	1.7e-54	72.2
QAS29594.1\|1290118_1291114_+	glycosyltransferase	S5FKN0	Shigella_phage	3.1e-52	38.8

DBSCAN-SWA_6

1296819 : 1305512

Staphylococcus_phage(50.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS29595.1\|1296819_1297887_+	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A1V0SE20	Indivirus	1.6e-38	33.9
QAS29596.1\|1297887_1298490_+	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	3.1e-23	38.9
QAS29597.1\|1298491_1299706_+	GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	3.1e-86	46.9
QAS29598.1\|1299702_1300182_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.0e-32	46.7
QAS29599.1\|1300212_1300722_-	nucleotidyltransferase family protein	NA	NA	NA	NA
QAS29600.1\|1300762_1301683_-\|transposase	IS30-like element ISLsa1 family transposase	Q9MBM9	Staphylococcus_prophage	7.1e-51	37.9
QAS29601.1\|1301986_1302550_-	hypothetical protein	NA	NA	NA	NA
QAS29602.1\|1302638_1303280_+	Crp/Fnr family transcriptional regulator	NA	NA	NA	NA
QAS29603.1\|1303415_1304042_-	TetR/AcrR family transcriptional regulator	NA	NA	NA	NA
QAS29604.1\|1304195_1305512_+	NADH peroxidase	A0A2K5B2C5	Erysipelothrix_phage	6.6e-10	25.0

DBSCAN-SWA_7

2131546 : 2179593

Lactobacillus_phage(53.12%)

tail,portal,holin,capsid,head,terminase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS31303.1\|2131546_2131924_-\|holin	holin	A0A2K9VCG4	Lactobacillus_phage	6.7e-16	67.5
QAS30354.1\|2131937_2132201_-\|holin	holin	E9LUR9	Lactobacillus_phage	6.9e-36	94.3
QAS30355.1\|2132200_2133379_-	LysM peptidoglycan-binding domain-containing protein	E9LUR8	Lactobacillus_phage	1.9e-194	87.5
QAS30356.1\|2134293_2136480_-	SGNH/GDSL hydrolase family protein	NA	NA	NA	NA
QAS30357.1\|2136469_2136736_-	hypothetical protein	NA	NA	NA	NA
QAS30358.1\|2136732_2137914_-	hypothetical protein	Q9T1A5	Listeria_phage	7.8e-18	24.4
QAS30359.1\|2137910_2138807_-\|tail	phage tail family protein	A8ATA8	Listeria_phage	2.4e-19	28.5
QAS30360.1\|2138816_2142893_-	tape measure protein	E9LUR1	Lactobacillus_phage	2.6e-81	43.9
QAS30361.1\|2142892_2143129_-	hypothetical protein	NA	NA	NA	NA
QAS30362.1\|2143197_2143710_-	hypothetical protein	NA	NA	NA	NA
QAS30363.1\|2143727_2144303_-\|tail	phage tail protein	NA	NA	NA	NA
QAS30364.1\|2144314_2144701_-	DUF3168 domain-containing protein	NA	NA	NA	NA
QAS30365.1\|2144706_2145258_-\|tail	phage tail protein	NA	NA	NA	NA
QAS31304.1\|2145247_2145571_-	hypothetical protein	NA	NA	NA	NA
QAS30366.1\|2145575_2145926_-\|head,tail	phage head-tail connector protein	NA	NA	NA	NA
QAS30367.1\|2145940_2147023_-\|capsid	phage capsid protein	A0A0P0HSG2	Acinetobacter_phage	2.3e-40	33.2
QAS30368.1\|2147036_2147684_-	DUF4355 domain-containing protein	NA	NA	NA	NA
QAS30369.1\|2147792_2148737_-\|head	phage head morphogenesis protein	NA	NA	NA	NA
QAS30370.1\|2148750_2150427_-\|portal	phage portal protein	D2IYW2	Enterococcus_phage	7.8e-64	32.4
QAS30371.1\|2150416_2151715_-\|terminase	PBSX family phage terminase large subunit	B7T0C6	Staphylococcus_virus	1.3e-114	50.4
QAS31305.1\|2151919_2152720_-\|terminase	terminase	V5URT8	Oenococcus_phage	4.9e-48	46.7
QAS30372.1\|2152838_2153159_-	hypothetical protein	A0A1J1J9Q7	Escherichia_phage	1.4e-17	46.2
QAS30373.1\|2153382_2153646_-	hypothetical protein	A0A1S5SBT9	Streptococcus_phage	2.6e-22	58.6
QAS30374.1\|2153614_2153794_-	hypothetical protein	E9LUP6	Lactobacillus_phage	1.0e-06	50.9
QAS30375.1\|2153833_2154592_-	hypothetical protein	NA	NA	NA	NA
QAS30376.1\|2155608_2156070_-	transcriptional regulator	D6PSV8	Lactobacillus_phage	1.1e-39	57.0
QAS30377.1\|2156197_2156365_-	hypothetical protein	E9LUP4	Lactobacillus_phage	2.6e-12	82.6
QAS30378.1\|2156361_2156814_-	DUF1642 domain-containing protein	A0A2K9VCG9	Lactobacillus_phage	3.5e-35	50.0
QAS30379.1\|2156886_2157315_-	hypothetical protein	K4I239	Lactobacillus_phage	3.1e-33	56.6
QAS30380.1\|2157280_2157571_-	hypothetical protein	A0A2K9VCZ2	Lactobacillus_phage	1.5e-28	70.8
QAS30381.1\|2157567_2157969_-	hypothetical protein	A0A2K9VC36	Lactobacillus_phage	1.2e-10	35.2
QAS30382.1\|2158271_2158652_-	hypothetical protein	NA	NA	NA	NA
QAS30383.1\|2158648_2159167_-	hypothetical protein	O03915	Lactobacillus_phage	1.7e-54	68.4
QAS30384.1\|2159163_2159451_-	hypothetical protein	NA	NA	NA	NA
QAS30385.1\|2159447_2160356_-	DnaD domain protein	NA	NA	NA	NA
QAS30386.1\|2160435_2161401_-	hypothetical protein	A6M982	Geobacillus_virus	5.3e-65	54.5
QAS30387.1\|2161412_2161943_-	hypothetical protein	E9LUU0	Lactobacillus_phage	4.6e-55	59.2
QAS30388.1\|2161963_2162077_-	hypothetical protein	NA	NA	NA	NA
QAS30389.1\|2162209_2162380_-	hypothetical protein	NA	NA	NA	NA
QAS30390.1\|2162447_2162960_-	transcriptional regulator	D6PST4	Lactobacillus_phage	3.2e-29	44.7
QAS30391.1\|2163027_2163333_-	DUF771 domain-containing protein	NA	NA	NA	NA
QAS30392.1\|2163527_2163758_-	XRE family transcriptional regulator	NA	NA	NA	NA
QAS30393.1\|2163930_2164293_+	helix-turn-helix domain-containing protein	A0A0U4KKM4	Exiguobacterium_phage	2.6e-09	32.0
QAS30394.1\|2164304_2164718_+	ImmA/IrrE family metallo-endopeptidase	A0A0M4RD70	Bacillus_phage	4.5e-05	31.3
QAS30395.1\|2164748_2166245_+	hypothetical protein	NA	NA	NA	NA
QAS30396.1\|2166767_2167103_+	hypothetical protein	A0A1B0Y4P2	Lactobacillus_phage	4.1e-25	52.2
QAS30397.1\|2167713_2169333_+	hypothetical protein	A0A1S5SA14	Streptococcus_phage	3.1e-94	48.2
QAS30398.1\|2171070_2171277_-	hypothetical protein	NA	NA	NA	NA
QAS30399.1\|2171694_2172000_-	hypothetical protein	NA	NA	NA	NA
QAS30400.1\|2172082_2172463_-	hypothetical protein	NA	NA	NA	NA
QAS30401.1\|2172611_2172881_-\|head,tail	phage gp6-like head-tail connector protein	NA	NA	NA	NA
QAS30402.1\|2173290_2174832_-\|capsid	phage major capsid protein	B8R651	Lactobacillus_phage	3.3e-45	25.8
QAS30403.1\|2174828_2175929_-\|portal	phage portal protein	A0A2H4J9Q5	uncultured_Caudovirales_phage	7.9e-49	34.3
QAS30404.1\|2175929_2176130_-	hypothetical protein	NA	NA	NA	NA
QAS30405.1\|2176083_2177787_-\|terminase	terminase large subunit	A0A2H4JBN3	uncultured_Caudovirales_phage	9.2e-121	40.1
QAS30406.1\|2177783_2178257_-\|terminase	phage terminase small subunit P27 family	NA	NA	NA	NA
QAS30407.1\|2178872_2179262_-	HNH endonuclease	A0A1J0MFT4	Staphylococcus_phage	8.8e-19	45.6
QAS30408.1\|2179254_2179593_-\|head,tail	head-tail adaptor protein	A0A2P0ZLF0	Lactobacillus_phage	1.0e-07	33.6

DBSCAN-SWA_8

2376822 : 2385336

Synechococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAS30575.1\|2376822_2377401_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.3e-21	33.9
QAS30576.1\|2377393_2378419_-	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	3.2e-60	41.0
QAS30577.1\|2378415_2379870_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	2.5e-50	34.0
QAS30578.1\|2379854_2382074_-	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	4.5e-144	39.3
QAS30579.1\|2382066_2382747_-	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
QAS30580.1\|2382746_2383001_-	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
QAS30581.1\|2383002_2383734_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	M4SM18	Cyanophage	1.2e-37	37.3
QAS30582.1\|2383736_2384867_-	5-(carboxyamino)imidazole ribonucleotide synthase	NA	NA	NA	NA
QAS30583.1\|2384850_2385336_-	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	2.3e-16	41.0

Anti-CRISPR protein detection

Acr_ID: CP035174.1|QAS28819.1|363801_364065_+|hypothetical-protein

Click the colored protein region to show detailed information

Acr_ID: CP035174.1|QAS28820.1|364166_364445_+|hypothetical-protein

Click the colored protein region to show detailed information

Acr ID

Acr position

Acr size

Homology with known anti

Neighbor HTH/AcRanker

Neighbor Aca

In prophage

Protospacer in prophage

CP035174.1|QAS28819.1|363801_364065_+|hypothetical-protein

363801_364065_+

87 aa aa

324145-368915

yes

CRISPR ID	CRISPR type	Spacer info	Spacer location	Spacer length	Protospacer location	Mismatch
CP035174_1	Orphan	1.2\|360321\|18\|CP035174\|CRT	360321-360338	18	360237-360254	2

1. spacer 1.2|360321|18|CP035174|CRT matches to CP035174 position: 360237-360254, mismatch: 2

gcggccaagaaacataag	CRISPR spacer
gccgctaagaaacataag	Protospacer
** **.************

CP035174.1|QAS28820.1|364166_364445_+|hypothetical-protein

364166_364445_+

92 aa aa

324145-368915

yes

CRISPR ID	CRISPR type	Spacer info	Spacer location	Spacer length	Protospacer location	Mismatch
CP035174_1	Orphan	1.2\|360321\|18\|CP035174\|CRT	360321-360338	18	360237-360254	2

1. spacer 1.2|360321|18|CP035174|CRT matches to CP035174 position: 360237-360254, mismatch: 2

gcggccaagaaacataag	CRISPR spacer
gccgctaagaaacataag	Protospacer
** **.************

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP035175

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP035174

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information