CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP022776	Ralstonia solanacearum strain T11 chromosome, complete genome	0 crisprs	PD-DExK,cas3,WYL,csa3,DEDDh,DinG	0	0	3	0
CP022777	Ralstonia solanacearum strain T11 plasmid unnamed, complete sequence	0 crisprs	csa3,PD-DExK	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

808439 : 818570

Hokovirus(14.29%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXW22794.1\|808439_810401_+	molecular chaperone DnaK	A0A1V0SH73	Hokovirus	8.6e-147	51.0
AXW22795.1\|810535_811678_+	molecular chaperone DnaJ	A0A0P0YMJ9	Yellowstone_lake_phycodnavirus	7.7e-23	32.7
AXW22796.1\|811713_813594_+	aminodeoxychorismate synthase component I	A0A0B5J984	Pandoravirus	1.0e-56	37.3
AXW22797.1\|813549_814236_-	ABC transporter ATP-binding protein	A0A1V0SE00	Indivirus	3.5e-10	22.7
AXW22798.1\|814243_814951_-	ABC transporter permease	NA	NA	NA	NA
AXW22799.1\|814958_815780_-	3-methyl-2-oxobutanoate hydroxymethyltransferase	A0A0N7HVJ8	Ostreococcus_lucimarinus_virus	5.4e-34	31.3
AXW22800.1\|815836_816481_-	deoxynucleoside kinase	A0A249XZR7	Enterococcus_phage	1.1e-05	28.1
AXW22801.1\|816513_817023_-	2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase	NA	NA	NA	NA
AXW22802.1\|817022_818570_-	polynucleotide adenylyltransferase PcnB	H7BUW3	unidentified_phage	2.5e-24	34.5

DBSCAN-SWA_2

1609870 : 1699965

Ralstonia_phage(40.0%)

portal,integrase,tRNA,tail,head,terminase,capsid,plate,holin

attL 1641568:1641585|attR 1676415:1676432

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXW23478.1\|1609870_1611778_+\|tRNA	threonine--tRNA ligase	A0A2K9L297	Tupanvirus	3.8e-123	37.4
AXW23479.1\|1611806_1612343_+	translation initiation factor IF-3	A0A2L0UZ54	Agrobacterium_phage	2.4e-11	29.9
AXW23480.1\|1612539_1612737_+	50S ribosomal protein L35	NA	NA	NA	NA
AXW23481.1\|1612763_1613120_+	50S ribosomal protein L20	NA	NA	NA	NA
AXW23482.1\|1613233_1614268_+\|tRNA	phenylalanine--tRNA ligase subunit alpha	A0A2H4UW22	Bodo_saltans_virus	9.5e-28	39.8
AXW23483.1\|1614334_1616764_+\|tRNA	phenylalanine--tRNA ligase subunit beta	NA	NA	NA	NA
AXW23484.1\|1616831_1617239_+	integration host factor subunit alpha	A0A1P8CWT5	Bacillus_phage	1.3e-12	42.2
AXW23485.1\|1617263_1617698_+	MerR family transcriptional regulator	NA	NA	NA	NA
AXW23486.1\|1618196_1619369_+	acyltransferase	NA	NA	NA	NA
AXW23487.1\|1619550_1620486_-	aldose epimerase	NA	NA	NA	NA
AXW23488.1\|1620544_1620736_+	hypothetical protein	NA	NA	NA	NA
AXW23489.1\|1620841_1621198_+	hypothetical protein	NA	NA	NA	NA
AXW23490.1\|1621331_1622420_+	DNA polymerase IV	NA	NA	NA	NA
AXW23491.1\|1622621_1624052_+	amino acid permease	NA	NA	NA	NA
AXW23492.1\|1624070_1624874_-	DUF1223 domain-containing protein	NA	NA	NA	NA
AXW23493.1\|1625172_1625460_+	DUF1488 domain-containing protein	NA	NA	NA	NA
AXW23494.1\|1625785_1626592_+	2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase	NA	NA	NA	NA
AXW23495.1\|1626617_1627082_-	homoprotocatechuate degradation operon regulator HpaR	NA	NA	NA	NA
AXW23496.1\|1627098_1627908_-	exodeoxyribonuclease III	NA	NA	NA	NA
AXW23497.1\|1628047_1628266_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AXW23498.1\|1628337_1631022_-	bifunctional DNA primase/helicase	A0A077K8T2	Ralstonia_phage	0.0e+00	94.6
AXW23499.1\|1631077_1631401_-	hypothetical protein	NA	NA	NA	NA
AXW23500.1\|1631420_1631624_-	hypothetical protein	A0A077K9T6	Ralstonia_phage	5.0e-18	67.6
AXW23501.1\|1631616_1631859_-	hypothetical protein	A4PE65	Ralstonia_virus	1.5e-29	84.0
AXW23502.1\|1632022_1632262_-	hypothetical protein	NA	NA	NA	NA
AXW23503.1\|1632354_1632603_-	transcriptional regulator	A0A077K829	Ralstonia_phage	2.3e-33	84.1
AXW23504.1\|1632664_1632874_-	hypothetical protein	NA	NA	NA	NA
AXW23505.1\|1632948_1633422_+	transcriptional regulator	A0A077K9Z1	Ralstonia_phage	7.3e-60	78.7
AXW23506.1\|1633545_1633917_+	hypothetical protein	A0A077K8S5	Ralstonia_phage	4.1e-58	87.0
AXW23507.1\|1633945_1634695_-	site-specific DNA-methyltransferase	A0A1S5NPU0	Burkholderia_phage	6.5e-95	68.1
AXW23508.1\|1634645_1634855_-	Com family DNA-binding transcriptional regulator	A0A1S5NPS9	Burkholderia_phage	2.4e-15	70.4
AXW23509.1\|1635172_1636300_-\|tail	phage tail protein	A4PE54	Ralstonia_virus	2.3e-197	94.4
AXW23510.1\|1636296_1636719_-	oxidoreductase	A4PE53	Ralstonia_virus	2.2e-71	96.4
AXW23511.1\|1636721_1639385_-\|tail	phage tail protein	A4PE52	Ralstonia_virus	0.0e+00	84.4
AXW23512.1\|1639381_1639483_-\|tail	GpE family phage tail protein	A0A077K821	Ralstonia_phage	2.7e-09	93.9
AXW23513.1\|1639479_1639806_-\|tail	phage tail assembly protein	A4PE51	Ralstonia_virus	9.8e-48	95.4
AXW23514.1\|1639881_1640391_-\|tail	phage major tail tube protein	A0A077KER7	Ralstonia_phage	9.2e-77	79.3
AXW23515.1\|1640422_1641598_-\|tail	phage tail protein	A0A077K9Y4	Ralstonia_phage	3.2e-221	96.9
1641568:1641585	attL	NA	NA	NA	NA
AXW23516.1\|1641724_1642477_-\|tail	phage tail protein	A4PE47	Ralstonia_virus	5.7e-107	83.2
AXW23517.1\|1642489_1644157_-\|tail	phage tail protein	A0A077K818	Ralstonia_phage	5.1e-265	83.7
AXW23518.1\|1644163_1644781_-\|tail	phage tail protein I	A0A077KER5	Ralstonia_phage	1.5e-100	96.2
AXW23519.1\|1644773_1645682_-\|plate	baseplate assembly protein	A0A077K9X9	Ralstonia_phage	9.8e-154	94.7
AXW23520.1\|1645684_1646032_-\|plate	baseplate assembly protein	A4PE42	Ralstonia_virus	1.0e-55	94.8
AXW23521.1\|1646028_1646646_-\|plate	phage baseplate assembly protein V	A4PE41	Ralstonia_virus	2.4e-103	89.8
AXW23522.1\|1646775_1647513_+	hypothetical protein	NA	NA	NA	NA
AXW23523.1\|1647517_1647964_-	phage virion morphogenesis protein	A4PE39	Ralstonia_virus	5.4e-65	89.7
AXW23524.1\|1647960_1648395_-\|tail	phage tail protein	A0A077K8R3	Ralstonia_phage	2.3e-68	88.2
AXW23525.1\|1648391_1648892_-	hypothetical protein	A0A077K9R7	Ralstonia_phage	4.2e-74	91.0
AXW23526.1\|1648888_1649692_-	peptidoglycan-binding protein	A4PE36	Ralstonia_virus	1.6e-120	83.8
AXW23527.1\|1649688_1650006_-\|holin	phage holin family protein	A4PE35	Ralstonia_virus	1.2e-42	89.1
AXW23528.1\|1650002_1650410_-	hypothetical protein	A0A077K9X1	Ralstonia_phage	9.8e-21	89.6
AXW23529.1\|1650425_1650629_-\|tail	phage tail protein	A0A077K8R0	Ralstonia_phage	4.2e-25	86.8
AXW23530.1\|1650628_1651108_-\|head	head completion/stabilization protein	A4PE32	Ralstonia_virus	6.4e-72	86.8
AXW23531.1\|1651222_1651927_-\|terminase	terminase	A4PE31	Ralstonia_virus	5.3e-91	76.9
AXW23532.1\|1651923_1652934_-\|capsid	phage major capsid protein, P2 family	A4PE30	Ralstonia_virus	2.0e-163	85.8
AXW23533.1\|1652983_1653826_-\|capsid	capsid scaffolding protein	A4PE29	Ralstonia_virus	1.7e-120	77.9
AXW23534.1\|1653962_1655744_+	oxidoreductase	A0A077K8Q7	Ralstonia_phage	0.0e+00	91.7
AXW23535.1\|1655740_1656826_+\|portal	phage portal protein	A0A077K9Q8	Ralstonia_phage	1.6e-195	91.7
AXW23536.1\|1656842_1657919_-	hypothetical protein	NA	NA	NA	NA
AXW23537.1\|1658297_1658561_-	hypothetical protein	NA	NA	NA	NA
AXW23538.1\|1658569_1660270_-	DUF2875 domain-containing protein	NA	NA	NA	NA
AXW23539.1\|1660263_1662564_-	DUF3274 domain-containing protein	A0A077K801	Ralstonia_phage	4.7e-19	26.4
AXW23540.1\|1662575_1665311_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	1.1e-91	33.4
AXW23541.1\|1665681_1666824_-	amino acid-binding protein	NA	NA	NA	NA
AXW23542.1\|1666952_1667204_-	hypothetical protein	NA	NA	NA	NA
AXW23543.1\|1667317_1668076_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
AXW25178.1\|1668378_1668642_-	hypothetical protein	NA	NA	NA	NA
AXW25179.1\|1668700_1669042_-	hypothetical protein	NA	NA	NA	NA
AXW23544.1\|1669474_1670206_-	hypothetical protein	NA	NA	NA	NA
AXW23545.1\|1670811_1672104_-\|integrase	integrase	Q6J1Q2	Burkholderia_virus	5.5e-134	58.7
AXW23546.1\|1672463_1673735_+	cytosine deaminase	NA	NA	NA	NA
AXW23547.1\|1673795_1675916_-	oligopeptidase A	A0A1V0SD92	Indivirus	1.4e-33	21.1
AXW23548.1\|1676035_1676905_-	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	A0A249XZQ2	Enterococcus_phage	1.0e-35	38.4
1676415:1676432	attR	NA	NA	NA	NA
AXW23549.1\|1677009_1677642_-	DNA-binding response regulator	NA	NA	NA	NA
AXW25180.1\|1677683_1680257_-	PAS domain-containing sensor histidine kinase	NA	NA	NA	NA
AXW23550.1\|1680572_1683263_+	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	NA	NA	NA	NA
AXW23551.1\|1683420_1685097_+	dihydrolipoyllysine-residue acetyltransferase	NA	NA	NA	NA
AXW23552.1\|1685187_1685763_+	MFS transporter	NA	NA	NA	NA
AXW23553.1\|1685817_1687599_+	dihydrolipoyl dehydrogenase	A0A2K5B2C5	Erysipelothrix_phage	1.5e-36	25.9
AXW23554.1\|1687695_1688253_-	hypothetical protein	NA	NA	NA	NA
AXW23555.1\|1688615_1689197_-	Phasin (PHA-granule associated protein)	NA	NA	NA	NA
AXW23556.1\|1689587_1690562_+	histone deacetylase	NA	NA	NA	NA
AXW23557.1\|1690558_1691476_+	EamA/RhaT family transporter	NA	NA	NA	NA
AXW23558.1\|1691541_1691985_+	thioesterase	NA	NA	NA	NA
AXW23559.1\|1691995_1692703_+	nitrobenzoate reductase	M1I6Q5	Acanthocystis_turfacea_Chlorella_virus	3.0e-25	34.4
AXW23560.1\|1693132_1694308_+	D-alanyl-D-alanine endopeptidase	NA	NA	NA	NA
AXW23561.1\|1694384_1695185_-	IclR family transcriptional regulator	NA	NA	NA	NA
AXW23562.1\|1695423_1696149_+	Fe-S oxidoreductase	NA	NA	NA	NA
AXW23563.1\|1696188_1697598_+	iron-sulfur cluster-binding protein	NA	NA	NA	NA
AXW23564.1\|1697594_1697918_+	hypothetical protein	NA	NA	NA	NA
AXW23565.1\|1697944_1698259_+	NINE protein	NA	NA	NA	NA
AXW23566.1\|1698458_1699232_-	arginyltransferase	NA	NA	NA	NA
AXW23567.1\|1699218_1699965_-\|tRNA	leucyl/phenylalanyl-tRNA--protein transferase	NA	NA	NA	NA

DBSCAN-SWA_3

2611219 : 2619451

Bacillus_virus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXW24285.1\|2611219_2612269_-	methionine ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	9.9e-25	33.0
AXW24286.1\|2612425_2613349_-	deacetylase	A0A2K9KZC4	Tupanvirus	6.4e-44	36.1
AXW24287.1\|2613466_2614579_+	lytic murein transglycosylase B	NA	NA	NA	NA
AXW24288.1\|2614658_2615561_-	cysteine synthase CysM	A0A1X9I5K7	Streptococcus_phage	6.3e-52	40.8
AXW24289.1\|2615664_2615982_-	competence protein ComE	NA	NA	NA	NA
AXW24290.1\|2616111_2617107_-	ADP-glyceromanno-heptose 6-epimerase	R9S880	Prochlorococcus_phage	6.3e-29	32.3
AXW24291.1\|2617103_2618051_-	D-glycero-beta-D-manno-heptose-7-phosphate kinase	A0A2H4N7X4	Lake_Baikal_phage	4.5e-16	27.3
AXW24292.1\|2618077_2619451_-	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	1.5e-76	38.9

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage